Updated on 2024/12/21

写真a

 
NISHIDA, Nao
 
Affiliation
Affiliated organization, Waseda Institute for Advanced Study
Job title
Assistant Professor(non-tenure-track)
Degree
PhD(Agriculture) ( 2014.03 Kyoto University )
Mail Address
メールアドレス

Research Experience

  • 2022.04
    -
    Now

    Waseda University   Institue for Advanced Study   Assistant Professor

  • 2021.09
    -
    2022.03

    Tokyo Medical University   Institute of Medical Science   Assistant Professor

  • 2017.04
    -
    2021.08

    Fred Hutchinson Cancer Center   Human Biology Division   Postdoctoral Fellow

  • 2017.04
    -
    2019.03

    Japan Society for the Promotion of Science   Overseas Research Fellow

  • 2014.04
    -
    2017.03

    Japan Society for the Promotion of Science   Research Fellow (PD)

  • 2014.04
    -
    2017.03

    National Cancer Center   Research Institute

  • 2012.04
    -
    2014.03

    Japan Society for the Promotion of Science   Research Fellow (DC2)

▼display all

Education Background

  • 2011.04
    -
    2014.03

    Kyoto University   Graduate School of Agriculture   Division of Applied Life Sciences  

    Doctoral course

  • 2009.04
    -
    2011.03

    Kyoto University   Graduate School of Agriculture   Division of Applied Life Sciences  

    Master's course

  • 2005.04
    -
    2009.03

    Kyoto University   Faculty of Agriculture   Division of Applied Life Sciences  

Committee Memberships

  • 2022.04
    -
    Now

    Japanese Society for Extracellular Vesicles  Director

Research Areas

  • Cell biology   Cell-cell interactions, extracellular vesicles, exosomes / Tumor biology   Tumor microenvironment

Research Interests

  • Tumor microenvironment

  • Cell-cell interaction

  • Organotypic culture

  • Extracellular vesicles

  • Exosome

  • Tumor-associated macrophage

▼display all

Awards

  • 卓越研究員候補

    2021   日本学術振興会  

  • ISEV2017 Junior Member Scholarship

    2017.05   International Society for Extracellular Vesicles  

  • 若手優秀発表賞

    2015.12   第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会  

 

Papers

  • Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progression.

    Tomofumi Yamamoto, Jun Nakayama, Fumihiko Urabe, Kagenori Ito, Nao Nishida-Aoki, Masami Kitagawa, Akira Yokoi, Masahiko Kuroda, Yutaka Hattori, Yusuke Yamamoto, Takahiro Ochiya

    Cell reports   43 ( 8 ) 114517 - 114517  2024.07  [Refereed]  [International journal]

    　View Summary

    Cancer cells secrete extracellular vesicles (EVs) to regulate cells in the tumor microenvironment to benefit their own growth and survive in the patient's body. Although emerging evidence has demonstrated the molecular mechanisms of EV release, regulating cancer-specific EV secretion remains challenging. In this study, we applied a microRNA library to reveal the universal mechanisms of EV secretion from cancer cells. Here, we identified miR-891b and its direct target gene, phosphoserine aminotransferase 1 (PSAT1), which promotes EV secretion through the serine-ceramide synthesis pathway. Inhibition of PSAT1 affected EV secretion in multiple types of cancer, suggesting that the miR-891b/PSAT1 axis shares a common mechanism of EV secretion from cancer cells. Interestingly, aberrant PSAT1 expression also regulated cancer metastasis via EV secretion. Our data link the PSAT1-controlled EV secretion mechanism and cancer metastasis and show the potential of this mechanism as a therapeutic target in multiple types of cancer.

    DOI PubMed

    Scopus

  • Impacts of tissue context on extracellular vesicles‐mediated cancer–host cell communications

    Nao Nishida‐Aoki, Takahiro Ochiya

    Cancer Science    2024.03  [Refereed]  [Invited]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

    2
    Citation
    (Scopus)

  • Polypharmacologic Reprogramming of Tumor-Associated Macrophages toward an Inflammatory Phenotype

    Nao Nishida-Aoki, Taranjit S. Gujral

    Cancer Research   82 ( 3 ) 433 - 446  2022.02  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    12
    Citation
    (Scopus)

  • Measuring real-time drug response in organotypic tumor tissue slices

    Nishida-Aoki, N., Bondesson, A.J., Gujral, T.S.

    Journal of Visualized Experiments   2020 ( 159 )  2020  [Refereed]  [Invited]

    Authorship：Lead author

    DOI

    Scopus

    15
    Citation
    (Scopus)

  • Lipidomic analysis of cells and extracellular vesicles from high-and low-metastatic triple-negative breast cancer

    Nishida-Aoki, N., Izumi, Y., Takeda, H., Takahashi, M., Ochiya, T., Bamba, T.

    Metabolites   10 ( 2 )  2020  [Refereed]  [International journal]

    Authorship：Lead author

    　View Summary

    Extracellular vesicles (EVs) are lipid bilayer nanovesicles secreted from almost all cells including cancer. Cancer-derived EVs contribute to cancer progression and malignancy via educating the surrounding normal cells. In breast cancer, epidemiological and experimental observations indicated that lipids are associated with cancer malignancy. However, lipid compositions of breast cancer EVs and their contributions to cancer progression are unexplored. In this study, we performed a widely targeted quantitative lipidomic analysis in cells and EVs derived from high- and low-metastatic triple-negative breast cancer cell lines, using supercritical fluid chromatography fast-scanning triple-quadrupole mass spectrometry. We demonstrated the differential lipid compositions between EVs and cells of their origin, and between high- and low-metastatic cell lines. Further, we demonstrated EVs from highly metastatic breast cancer accumulated unsaturated diacylglycerols (DGs) compared with EVs from lower-metastatic cells, without increasing the amount in cells. The EVs enriched with DGs could activate the protein kinase D signaling pathway in endothelial cells, which can lead to stimulated angiogenesis. Our results indicate that lipids are selectively loaded into breast cancer EVs to support tumor progression.

    DOI PubMed

    Scopus

    58
    Citation
    (Scopus)

  • Altered biodistribution of deglycosylated extracellular vesicles through enhanced cellular uptake

    Nishida-Aoki, N., Tominaga, N., Kosaka, N., Ochiya, T.

    Journal of Extracellular Vesicles   9 ( 1 )  2020  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    63
    Citation
    (Scopus)

  • Organotypic tumor slice cultures provide a versatile platform for immuno-oncology and drug discovery

    Sivakumar, R., Chan, M., Shin, J.S., Nishida-Aoki, N., Kenerson, H.L., Elemento, O., Beltran, H., Yeung, R., Gujral, T.S.

    OncoImmunology   8 ( 12 )  2019  [Refereed]

    DOI

    Scopus

    58
    Citation
    (Scopus)

  • Emerging approaches to study cell–cell interactions in tumor microenvironment

    Nishida-Aoki, N., Gujral, T.S.

    Oncotarget   10 ( 7 )  2019  [Refereed]

    Authorship：Lead author

  • Disruption of Circulating Extracellular Vesicles as a Novel Therapeutic Strategy against Cancer Metastasis

    Nishida-Aoki, N., Tominaga, N., Takeshita, F., Sonoda, H., Yoshioka, Y., Ochiya, T.

    Molecular Therapy   25 ( 1 )  2017  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    176
    Citation
    (Scopus)

  • Activation of the mitochondrial signaling pathway in response to organic solvent stress in yeast

    Nishida-Aoki, N., Mori, H., Kuroda, K., Ueda, M.

    Current Genetics   61 ( 2 )  2015  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    15
    Citation
    (Scopus)

  • Interactions between cancer cells and normal cells via miRNAs in extracellular vesicles

    Nishida-Aoki, N., Ochiya, T.

    Cellular and Molecular Life Sciences   72 ( 10 )  2015  [Refereed]  [Invited]

    Authorship：Lead author

    DOI

    Scopus

    50
    Citation
    (Scopus)

  • A design for the control of apoptosis in genetically modified Saccharomyces cerevisiae

    Nao Nishida, Misa Noguchi, Kouichi Kuroda, Mitsuyoshi Ueda

    Bioscience, Biotechnology, and Biochemistry   78 ( 2 ) 358 - 362  2014.02  [Refereed]

    Authorship：Lead author

    　View Summary

    <jats:title>Abstract</jats:title>
    <jats:p>We have engineered a system that holds potential for use as a safety switch in genetically modified yeasts. Human apoptotic factor BAX (no homolog in yeast), under the control of the FBP1 (gluconeogenesis enzyme) promoter, was conditionally expressed to induce yeast cell apoptosis after glucose depletion. Such systems might prove useful for the safe use of genetically modified organisms.</jats:p>

    DOI

    Scopus

    3
    Citation
    (Scopus)

  • Activation of signaling pathways related to cell wall integrity and multidrug resistance by organic solvent in Saccharomyces cerevisiae

    Nishida, N., Jing, D., Kuroda, K., Ueda, M.

    Current Genetics   60 ( 3 )  2014  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    26
    Citation
    (Scopus)

  • ABC transporters and cell wall proteins involved in organic solvent tolerance in Saccharomyces cerevisiae

    Nishida, N., Ozato, N., Matsui, K., Kuroda, K., Ueda, M.

    Journal of Biotechnology   165 ( 2 )  2013  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    28
    Citation
    (Scopus)

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Books and Other Publications

  • 「留学する?」から一歩踏み出す研究留学実践ガイド : 人生の選択肢を広げよう : ラボの探し方・応募からその後のキャリア展開まで、57人が語る等身大のアドバイス

    山本, 慎也, 中田, 大介( Part： Contributor, コーヒーと雨のエメラルドシティで新米PIとともに4年間を過ごして)

    羊土社  2024.09 ISBN: 9784758122733

  • 医療を変えるエクソソーム : 生体機能から疾患メカニズム、臨床応用まで

    落谷, 孝広, 吉岡, 祐亮( Part： Contributor, 26章 エクソソームを応用した治療法の開発)

    化学同人  2018.08 ISBN: 9784759819786

  • 直径100nmのメッセンジャーエクソソームは診断・治療に革命をもたらすか? : 特集

    吉岡, 祐亮, 落谷, 孝広( Part： Contributor, 6. エクソソームが築く次世代の医療)

    羊土社  2016.06 ISBN: 9784758101523

Presentations

  • Investigating the impact of the surface and environmental factors on cancer extracellular vesicle delivery in tumor tissues

    Nao Nishida-Aoki  [Invited]

    ISEV-ISCT Joint Satellite Meeting at ISEV2024 

    Presentation date： 2024.05

  • Influence of tissue environment on cancer cell traits and extracellular vesicle transfer

    Nao Nishida-Aoki  [Invited]

    The 9th Annual Meeting Japanese Society for Extracellular Vesicles 

    Presentation date： 2022.10

  • Monitoring cancer extracellular vesicle transfer within tumor tissue context

    Nao Nishida-Aoki

    ISEV2024 

    Presentation date： 2024.05

  • がん組織スライス培養法を用いた薬効評価および細胞間相互作用の理解

    西田 奈央  [Invited]

    武田薬品工業株式会社 GAPセミナー 

    Presentation date： 2024.02

  • 組織スライス培養法を用いたがん組織内における細胞間相互作用の理解

    西田 奈央  [Invited]

    名古屋大学第177 回 創薬科学 セミナー 

    Presentation date： 2024.01

  • Elucidating cancer cell-normal cell interactions within tissue context

    Nao Nishida-Aoki  [Invited]

    Rosalind Franklin University of Medicine and Science (Virtual). 

    Presentation date： 2024.01

  • For healthier and sustainable life: studying cell-cell interactions within tumor microenvironment

    Nao Nishida-Aoki  [Invited]

    ZUKO-WIAS joint workshop “Basic Sciences for Sustainable Development” 

    Presentation date： 2023.12

  • 組織スライスモデルを用いたがん微小環境内におけるがん細胞外小胞の受け渡しの理解

    西田 奈央  [Invited]

    BioMedical Forum 2024 大阪公立大学 

    Presentation date： 2023.12

  • アカデミアパスを進むー医学系への転身、 単身赴任留学に育児、そしてこれから

    西田 奈央  [Invited]

    第75回生物工学会大会 

    Presentation date： 2023.09

  • 細胞外小胞の治療応用のためのストラテジー および現状と課題

    西田 奈央  [Invited]

    第３０回日本遺伝子診療学会大会・第8回クリニカルバイオバンク学会シンポジウム 

    Presentation date： 2023.07

  • Understanding extracellular vesicle transfer within tumor tissue context

    Nao Nishida-Aoki  [Invited]

    2023 Japan-America Frontiers of Engineering (JAFOE) Symposium 

    Presentation date： 2023.07

  • Elucidating cancer cell-normal cell interactions within tissue context

    Nao Nishida-Aoki  [Invited]

    University of Mississippi Medical Center invited seminar (virtual) 

    Presentation date： 2023.06

  • Evaluating stromal contribution to therapeutic responses using organotypic t issue slice culture

    Nao Nishida-Aoki

    The 81st Annual Meeting of the Japanese Cancer Association 

    Presentation date： 2022.10

  • がん組織スライス培養を⽤いた 細胞外マトリクス阻害剤のスクリーニング

    西田 奈央

    第8回Matricellフォーラム 

    Presentation date： 2022.09

  • Toward spatiotemporal understanding of the tumor microenvironment modulated by extracellular vesicles

    Nao Nishida-Aoki  [Invited]

    32nd Forum in DOJIN 

    Presentation date： 2022.09

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Research Projects

  • Mechanisms of inducible secretion of cancer extracellular vesicles upon cell recognition and contribution to cancer malignancy

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research

    Project Year :

    2023.04
    -
    2026.03
     

  • がん組織内における細胞外小胞のヘテロな分布の検出

    公益財団法人 上原記念生命科学財団  上原記念生命科学財団研究奨励金

    Project Year :

    2024.04
    -
    2025.03
     

  • がん由来細胞外小胞を取り込む細胞の組織内分布の解析

    公益財団法人 三菱財団  三菱財団自然科学研究助成

    Project Year :

    2023.10
    -
    2024.09
     

  • Understanding extracellular vesicles modulating extracellular matrix in tumor tissue

    The Noguchi Institute 

    Project Year :

    2023.04
    -
    2024.03
     

    Nao Nishida-Aoki

  • がん組織環境中の細胞外小胞を介した相互作用の時空間的解析

    日本学術振興会  科学研究費助成事業 研究活動スタート支援

    Project Year :

    2022.08
    -
    2024.03
     

    西田 奈央

  • Identifying molecular switch of tumor-associated macrophage polarization

    Project Year :

    2023.01
    -
    2023.12
     

    Nao Nishida-Aoki

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Misc

  • 環境によって良くも悪くもふるまうがん細胞

    西田 奈央

    生物工学会誌   101 ( 2 ) 86  2023.02

    Authorship：Lead author

    DOI

  • Composition and Biological Activities of Exosomal Lipids Bilayers

    Nao NISHIDA-AOKI

    Oleoscience   21 ( 2 ) 69 - 75  2021.02

    Authorship：Lead author

    DOI

  • Multilateral strategies utilizing exosomes for cancer therapy

    Nishida-Aoki, N., Ochiya, T.

    Japanese Journal of Cancer and Chemotherapy   44 ( 5 ) 367 - 372  2017.05

    Authorship：Lead author

  • The mechanism of organic-solvent tolerance in yeast Saccharomyces cerevisiae triggered by a mutated transcription factor

    Kouichi Kuroda, Nao Nishida, Mitsuyoshi Ueda

    NEW BIOTECHNOLOGY   33   S59 - S59  2016.07

    Research paper, summary (international conference)  

    DOI

  • Variety of biological phenomena brought by exosomes

    Nao Nishida-Aoki, Takahiro Ochiya

      16 ( 2 ) 107 - 116  2015.06

    Authorship：Lead author

  • Lipid Bilayer Exosome–mediated Disease Diagnosis and Treatment Utilizing Exosomes

    Nao Nishida, Takahiro Ochiya

    membrane   40 ( 5 ) 235 - 241  2015

    Authorship：Lead author

    DOI

  • Organic solvent-activated signaling pathways via cell surface sensors and transcription factors

    Kouichi Kuroda, Nao Nishida, Mitsuyoshi Ueda

    FASEB JOURNAL   28 ( 1 )  2014.04

    Research paper, summary (international conference)  

  • Relationships between cancer cells and normal cells by transfer of miRNA via extracellular vesicles

    Nao Nishida-Aoki, Takahiro Ochiya

    Antisense   18 ( 2 ) 3 - 12  2014

    Authorship：Lead author

  • Effects of recognition sequence variations on transcription regulation of multidrug resistance regulator Pdr1p in yeast

    Nao Nishida, Kouichi Kuroda, Mitsuyoshi Ueda

    FASEB JOURNAL   27  2013.04

    Research paper, summary (international conference)  

  • ABC transporters and cell wall proteins involved in yeast organic solvent tolerance

    Nao Nishida, Naoki Ozato, Jing Dongyu, Kouichi Kuroda, Mitsuyoshi Ueda

    NEW BIOTECHNOLOGY   29   S104 - S104  2012.09

    Research paper, summary (international conference)  

    DOI

  • Functional analysis of Pdr1p in multidrug resistance and organic-solvent tolerance in yeast

    Nao Nishida, Naoki Ozato, Mitsuaki Sanada, Kouichi Kuroda, Mitsuyoshi Ueda

    FASEB JOURNAL   25  2011.04

    Research paper, summary (international conference)  

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Syllabus

 

Sub-affiliation

  • Faculty of Science and Engineering   School of Advanced Science and Engineering

Internal Special Research Projects

  • 位置情報を保持したがん組織内の細胞外小胞の捕捉基盤の開発

    2023   安井隆雄

    　View Summary

    がん細胞を含むほぼ全ての細胞はエクソソームを含む細胞外小胞を受け渡し、周囲の細胞と情報交換を行っている。本課題では、がん組織内で活発に交換される細胞外小胞の分布を調べる目的で、空間情報を維持したままがん組織の細胞外小胞を直接捕捉し、解析するための手法の開発検討を実施した。学外の共同研究者が開発した基盤の検討を行い、マウスモデル由来のがん組織を用いて、細胞外小胞の捕捉および検出が可能であることを示した。さらなる染色条件の検討が必要であるが、今後この基盤を用いて、がん組織内の不均一な細胞外小胞の偏りを検出し、細胞外小胞の分布によるがん細胞の悪性化への貢献を調べる予定である。

  • がん由来細胞外小胞の組織形成や維持における役割の解明

    2022  

    　View Summary

    本課題では、がん組織内で活発に交換されるエクソソームを含む細胞外小胞(Extracellular vesicles,EV)が、がん組織の形成や機能維持に果たす役割を明らかにする目的で研究を行った。がん細胞株と正常細胞株を用いた共培養系を用いて、当初持っていた仮説を実証するデータを得ることができ、さらに新たな仮説を得た。この予備実験のデータを元に科研費課題を提案し資金を獲得することができた。また、今後研究を進める上で必要となるEVの分泌阻害剤の、最適な種類と添加条件の検討を行った。本課題により、他の研究課題と相補的な位置付けで、がん組織内のEVを介した細胞間相互作用を理解する上で役立つ最初のデータを得ることができた。