Updated on 2024/05/08

写真a

 
HAYASHI, Hiroki
 
Affiliation
Faculty of Science and Engineering, School of Advanced Science and Engineering
Job title
Assistant Professor(non-tenure-track)
Degree
博士（工学） ( 2021.03 早稲田大学 )

Research Experience

  • 2023.04
    -
    Now

    Waseda University   Department of Applied Chemistry, School of Advanced Science and Engineering, Faculty of Science and Engineering   Assistant Professor

  • 2021.04
    -
    2023.03

    Waseda University   Department of Applied Chemistry, School of Advanced Science and Engineering   Assistant Professor

Education Background

  • 2018.04
    -
    2021.03

    Waseda University   Graduate School of Advanced Science and Engineering   Department of Nanoscience and Nanoengineering  

Professional Memberships

  • 2020.02
    -
    Now

    化学センサ研究会

  • 2018.08
    -
    Now

    電気化学会

Research Areas

  • Energy chemistry / Nanobioscience

Awards

  • 水野賞

    2021.03   早稲田大学   生体分子間相互作用に基づく分子認識界面により機能化した電界効果トランジスタバイオセンサ

  • 優秀賞および異分野賞

    2019.09   第2回COI学会   簡便なストレスマーカー検出のための半導体センサ界面の構築とバイオセンシングシステム

  • 優秀ポスター賞

    2018.10   第18回Conference for BioSignal and Medicine (CBSM)   インフルエンザウイルスの宿主域識別に向けた糖鎖固定化半導体センサの評価

  • Best Presentation Award

    2018.06   International Symposium on Biological Material Science for Agriculture and Engineering- Aiming at Future Interdisciplinary Collaborations   Discrimination of influenza virus subtypes in nasal mucus using glycan-immobilized field effect transistor biosensor

 

Papers

  • Field-effect transistor biosensor with signal amplification by ternary initiation complexes for detection of wide-range RNA concentration

    Hiroki Hayashi, Akihiro Enami, Hiroto Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masayasu Kuwahara, Tetsuya Osaka, Toshiyuki Momma

    Talanta   273   125846  2024.06  [Refereed]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

  • Semiconductor-based biosensor exploiting competitive adsorption with charged pseudo-target molecules for monitoring 5-fluorouracil concentration in human serum

    Hiroki Hayashi, Mayuri Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masahisa Okada, Futoshi Shibasaki, Tetsuya Osaka, Toshiyuki Momma

    Sensors and Actuators B: Chemical   395   134495 - 134495  2023.11  [Refereed]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

    1
    Citation
    (Scopus)

  • A Non‐Destructive Electrical Assay of Stem Cell Differentiation Based on Semiconductor Biosensing

    Sho Hideshima, Hiroki Hayashi, Sayoko Saito, Hiroaki Tateno, Toshiyuki Momma, Tetsuya Osaka

    Analysis & Sensing   3 ( 2 ) e202200046  2022.10  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    2
    Citation
    (Scopus)

  • Improvement in long-term stability of field effect transistor biosensor in aqueous environments using a combination of silane and reduced graphene oxide coating

    Sho Hideshima, Hiroki Hayashi, Ryo Takeuchi, Shofarul Wustoni, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Tetsuya Osaka

    Microelectronic Engineering   264   111859 - 111859  2022.08  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    2
    Citation
    (Scopus)

  • Potassium-regulated Immobilization of Cortisol Aptamer for Field-effect Transistor Biosensor to Detect Changes in Charge Distribution with Aptamer Transformation

    S. Kuroiwa, H. Hayashi, R. Toyama, N. Kaneko, K. Horii, K. Ohashi, T. Momma, T. Osaka

    Chemistry Letters   50 ( 5 ) 892 - 895  2021.05  [Refereed]

    Authorship：Lead author

    　View Summary

    Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guanine-quadruplex during the immobilization of the aptamers in a highly concentrated solution of K+.

    DOI

    Scopus

    5
    Citation
    (Scopus)

  • Immobilization of Target-Bound Aptamer on Field Effect Transistor Biosensor to Improve Sensitivity for Detection of Uncharged Cortisol

    Hiroki Hayashi, Ryo Toyama, Ryota Takibuchi, Sho Hideshima, Shigeki Kuroiwa, Naoto Kaneko, Katsunori Horii, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

    ELECTROCHEMISTRY   89 ( 2 ) 134 - 137  2021  [Refereed]

    Authorship：Lead author

    　View Summary

    Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors. (C) The Author(s) 2020. Published by ECSJ.

    DOI

    Scopus

    8
    Citation
    (Scopus)

  • Tetrameric jacalin as a receptor for field effect transistor biosensor to detect secretory IgA in human sweat

    Hiroki Hayashi, Naoki Sakamoto, Sho Hideshima, Yoshitaka Harada, Mika Tsuna, Shigeki Kuroiwa, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

    JOURNAL OF ELECTROANALYTICAL CHEMISTRY   873   114371  2020.09  [Refereed]

    Authorship：Lead author

    　View Summary

    Secretory immunoglobulin A (s-IgA), found in biological fluids, is useful for monitoring condition on mental health to prevent depression. In this study, the non-invasive detection of s-IgA in human sweat was demonstrated using field effect transistor (FFT) bioscnsors modified with a plant lectin, jacalin, as a receptor. The s-IgA molecules were detected with greater sensitivity using the jacalin-immobilized FET biosensors as compared to the sensitivity shown by Fabimmobilized FET bioscnsors. Jacalin, which is a small lectin tetramcr, has four glycan-binding sites and can capture a large number of s-IgA molecules within the charge-detectable region in terms of Dcbye length. Moreover, the jacalin-immobilized FET bioscnsor could detect s-IgA at concentrations ranging from 0.1 mu g/ml. to 100 mu g/mL Additionally, by using a filtration process to eliminate the interference of other components found in human sweat, our FET sensing system could specifically and quantitatively detect s-IgA. Therefore, our results show the utility of this device in monitoring mental stress.

    DOI

    Scopus

    12
    Citation
    (Scopus)

  • Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles

    Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka

    SCIENTIFIC REPORTS   9  2019.08  [Refereed]

    　View Summary

    Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.

    DOI

    Scopus

    32
    Citation
    (Scopus)

  • Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor

    S. Hideshima, S. Wustoni, M. Kobayashi, H. Hayashi, S. Kuroiwa, T. Nakanishi, T. Osaka

    Sensing and Bio-Sensing Research   17   25 - 29  2018.02  [Refereed]

    DOI

    Scopus

    15
    Citation
    (Scopus)

▼display all

Presentations

  • 亜鉛二次電池用正極への適用に向けた NMC111の脱リチウム処理の挙動

    林宏樹, 甲崎孝裕, 奈良洋希, 浅野剛太, 門間聰之

    電気化学会 第91回大会 

    Presentation date： 2024.03

    Event date：
    2024.03
    　
    　

  • 競合法を利用した電界効果トランジスタバイオセンサによる５-フルオロウラシルの検出

    藤田真佑里, 林 宏樹, 石川朱音, 黒岩繁樹, 大橋啓之, 岡田政久, 芝崎 太, 逢坂哲彌, 門間聰之

    2023電気化学秋季大会（第73回化学センサ研究発表会） 

    Presentation date： 2023.09

    Event date：
    2023.09
    　
    　

  • 等温核酸増幅法を適用したアンペロメトリック センサによる RNAの検出

    佐瀬 弘, 林 宏樹, 藤田 博仁, 片岡 由佳, 桒原 正靖, 門間 聰之

    2023電気化学秋季大会（第73回化学センサ研究発表会） 

    Presentation date： 2023.09

    Event date：
    2023.09
    　
    　

  • Highly sensitive RNA detection using field effect transistor biosensor with isothermal nucleic acid amplification

    Hiroki Hayashi, Akihiro Enami, Hiroto Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masayasu Kuwahara, Toshiyuki Momma, Tetsuya Osaka

    33rd Anniversary World Congress on Biosensors 2023 

    Presentation date： 2023.06

    Event date：
    2023.06
    　
    　

  • iPS細胞の分化状態把握に向けたFETバイオセンサによる未分化マーカーの検出

    秀島 翔, 林 宏樹, 舘野 浩章, 門間 聰之, 逢坂 哲彌

    電気化学会第90回大会（第72回化学センサ研究発表会） 

    Presentation date： 2023.03

    Event date：
    2023.03
    　
    　

  • Design of semiconductor-based biosensing system for uncharged molecule detection

    Hiroki Hayashi

    8th DGIST-Waseda Workshop on Electrochemistry 2022 

    Presentation date： 2022.11

  • 水系亜鉛負極電池用正極への三元系層状化合物の適用

    林宏樹, 浅野剛太, 三栗谷仁, 門間聰之, 逢坂哲彌

    第63回電池討論会 

    Presentation date： 2022.11

    Event date：
    2022.11
    　
    　

  • Application of Isothermal Nucleic Acid Amplification to Semiconductor Biosensor toward Highly Sensitive RNA Detection

    Presentation date： 2022.09

    Event date：
    2022.09
    　
    　

  • 電界効果トランジスタバイオセンサを用いた抗がん剤５-フルオロウラシルの検出

    林宏樹, 黒岩繁樹, 大橋啓之, 門間聰之, 逢坂哲彌, 岡田政久, 芝崎太

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第10回研究交流フォーラム 

    Presentation date： 2022.06

  • 人と動物の唾液ストレスセンサ

    大橋啓之, 黒岩繁樹, 林宏樹, 小坂田彩加, 門間聰之, 逢坂哲彌

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第10回研究交流フォーラム 

    Presentation date： 2022.06

  • Detection of 5-Fluorouracil in Serum by Semiconductor Biosensor Using Competition with Pseudoantigen

    Presentation date： 2022.03

    Event date：
    2022.03
    　
    　

  • 受容体分子の変形に伴う電荷分布の変化を利用したコルチゾールアプタマー固定化電界効果トランジスタバイオセンサ

    黒岩繁樹, 林宏樹, 遠山良, 大橋啓之, 門間聰之, 逢坂哲彌

    電気化学会第88回大会（第68回化学センサ研究発表会） 

    Presentation date： 2021.03

    Event date：
    2021.03
    　
    　

  • Detection of Uncharged 5-Fluorouracil Exploiting Sequential Adsorption of 5-Fluorouracil-Modified Bovine Serum Albumin Using Field Effect Transistor Biosensor

    M. Fujita, H. Hayashi, S. Kuroiwa, K. Ohashi, T. Momma, T. Osaka, M. Okada, F. Shibasaki

    Pacific Rim Meeting (PRIME) on Electrochemical and Solid State Science 2020 

    Presentation date： 2020.10

  • Saliva Stress Marker Monitor Using Aptamer Immobilized FET Biosensor

    K. Ohashi, S. Kuroiwa, R. Toyama, H. Hayashi, T. Momma, T. Osaka

    Pacific Rim Meeting (PRIME) on Electrochemical and Solid State Science 2020 

    Presentation date： 2020.10

  • Detection of Stress-Related Secretory IgA in Human Sweat Using Lectin-Immobilized Field Effect Transistor Biosensor

    H. Hayashi, N. Sakamoto, S. Hideshima, Y. Harada, M. Tsuna, S. Kuroiwa, K. Ohashi, T. Momma, T. Osaka

    Pacific Rim Meeting (PRIME) on Electrochemical and Solid State Science 2020 

    Presentation date： 2020.10

  • 非侵襲なストレスセンサに向けた半導体バイオセンサの作製

    黒岩繁樹, 林宏樹, 坂本尚輝, 原田義孝, 綱美香, 大橋啓之, 門間聰之

    第3回COI学術交流会 

    Presentation date： 2020.07

  • FETバイオセンサによる非荷電分子検出の感度向上を目的としたターゲット-アプタマー複合体を用いた認識界面の構築

    遠山良, 黒岩繁樹, 林宏樹, 大橋 啓之, 門間聰之, 逢坂哲彌

    2019年電気化学秋季大会（第66回化学センサ研究発表会）  (山梨) 

    Presentation date： 2019.09

  • 簡便なストレスマーカー検出のための半導体センサ界面の構築とバイオセンシングシステム

    林宏樹

    第2回COI学会  (東京) 

    Presentation date： 2019.09

  • Functionalization of Semiconductor-Based Biosensor by Glycan toward the Detection of Influenza Virus

    H. Hayashi

    NIMS-Waseda Joint Symposium  (東京) 

    Presentation date： 2019.07

  • Semiconductor-Based Portable Biosensor for Food Allergen Detection

    S. Hideshima, S. Kuroiwa, H. Hayashi, Y. Harada, M. Tsuna, T. Momma, T. Osaka

    The IEEE International Symposium on Circuits and Systems 2019 (ISCAS2019)  (北海道) 

    Presentation date： 2019.06

  • ストレスマーカーの低侵襲測定に向けたアプタマー固定化電界効果トランジスタセンサの作製

    遠山良, 林宏樹, 黒岩繁樹, 秀島翔, 大橋啓之, 門間聰之, 逢坂哲彌

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第8回研究交流フォーラム  (東京) 

    Presentation date： 2019.05

  • 小型受容体ジャカリンを用いた電界効果トランジスタバイオセンサによる分泌型IgAの検出

    坂本尚輝, 林宏樹, 佐藤慎, 秀島翔, 原田義孝, 綱美香, 黒岩繁樹, 門間聰之, 逢坂哲彌

    電気化学会第86回大会（第65回化学センサ研究発表会）  (京都) 

    Presentation date： 2019.03

  • Discrimination of Influenza virus subtypes in mucus samples using glycan-immobilized semiconductor-based biosensor

    H. Hayashi, T. Momma, T. Osaka

    6th DGIST-Waseda Workshop on Electrochemistry 2018  (大邱) 

    Presentation date： 2018.11

  • インフルエンザウイルスの宿主域識別に向けた糖鎖固定化半導体センサの評価

    林宏樹, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 門間聰之, 逢坂哲彌

    第18回Conference for BioSignal and Medicine (CBSM)  (神奈川) 

    Presentation date： 2018.10

  • コンゴーレッド固定化FETバイオセンサを用いたヒト血清中アミロイドβ凝集体の検出

    黒岩繁樹, 林宏樹, 秀島翔, 門間聰之, 逢坂哲彌

    2018年電気化学秋季大会（第64回化学センサ研究発表会）  (石川) 

    Presentation date： 2018.09

  • Detection of Whole Influenza Viral Particle in High Ionic Strength Solution by using Glycan-Immobilized Field Effect Transistor Biosensor

    S. Hideshima, H. Hayashi, S. Kuroiwa, T. Osaka

    The 12th International Symposium on Electrochemical Micro & Nano System Technologies (EMNT2018)  (ミラノ) 

    Presentation date： 2018.09

  • Detection of Influenza virus in nasal mucus by viscosity reduction using glycan-immoobilized FET biosensor

    H. Hayashi, S. Hideshima, H. Hinou, SI. Nishimura, Y. Sakoda, S. Kuroiwa, T. Nakanishi, T. Momma, T. Osaka

    28th Anniversary World Congress on Biosensors 2018  (フロリダ) 

    Presentation date： 2018.06

  • Discrimination of influenza virus subtypes in nasal mucus using glycan-immobilized field effect transistor biosensor

    H. Hayashi, S. Hideshima, S. Kuroiwa, T. Momma, T. Osaka

    International Symposium on Biological Material Science for Agriculture and Engineering- Aiming at Future Interdisciplinary Collaborations  (東京) 

    Presentation date： 2018.06

  • 電界効果トランジスタバイオセンサを用いたアミロイドβ凝集体の検出

    林宏樹, 秀島翔, 黒岩繁樹, 大橋啓之, 門間聰之, 逢坂哲彌

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第7回研究交流フォーラム  (東京) 

    Presentation date： 2018.05

  • Development of glycan-immobilized FET biosensor toward the detection of Influenza virus from biological sample

    H. Hayashi, S. Hideshima, S. Kuroiwa, T. Nakanishi, T. Momma, T. Osaka

    5th DGIST-Waseda Workshop on Electrochemistry 2017  (東京) 

    Presentation date： 2017.12

  • 糖鎖固定化電界効果トランジスタバイオセンサによるインフルエンザウイルス粒子の高感度検出

    林宏樹, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 門間聰之, 逢坂哲彌

    Conference for BioSignal and Medicine (CBSM) 第16回大会  (大分) 

    Presentation date： 2016.09

▼display all

Research Projects

  • 核酸増幅反応産物を介した汎用的な RNA 検出半導体バイオセンサの創製

    精密測定技術振興財団  精密測定技術振興のための調査･研究事業

    Project Year :

    2024.01
    -
    2025.03
     

  • 核酸増幅反応を促進する界面を具備したRNA検出電気化学センサ

    日本学術振興会  科学研究費助成事業 若手研究

    Project Year :

    2023.04
    -
    2025.03
     

    林 宏樹

  • 核酸増幅法による人工酵素DNAの多数形成を利用したRNA検出用電気化学バイオセンサの開発

    公益財団法人みずほ学術振興財団  第65回工学研究助成

    Project Year :

    2022.04
    -
    2023.03
     

  • コルチゾール検出に向けた人工酵素DNAアプタマーを利用した電気化学バイオセンサの開発

    公益財団法人里見奨学会  里見賞 研究提案表彰

    Project Year :

    2021.04
    -
    2022.03
     

  • 病原性ウイルスによる感冒の早期診断に向けた通信デバイスの開発に関する調査研究

    国立研究開発法人 科学技術振興機構  COI プログラム「COI 若手連携研究ファンド デジタル分野・FS」

    Project Year :

    2018.11
    -
    2019.03
     

Misc

  • 競合法を利用した電界効果トランジスタバイオセンサによる5-フルオロウラシルの検出

    藤田真佑里, 林宏樹, 石川朱音, 黒岩繁樹, 大橋啓之, 岡田政久, 芝崎太, 逢坂哲彌, 門間聰之

    Proceedings of the 73rd Chemical Sensor Symposium   39 ( Supplement B ) 85 - 87  2023.09

  • 等温核酸増幅法を適用したアンペロメトリックセンサによるRNAの検出

    佐瀬弘, 林宏樹, 藤田博仁, 片岡由佳, 桒原正靖, 門間聰之

    Proceedings of the 73rd Chemical Sensor Symposium   39 ( Supplement B ) 67 - 69  2023.09

  • DETECTION OF BIOMARKER OF UNDIFFERENTIATED HUMAN INDUCED PLURIPOTENT STEM CELLS BY USING FET BIOSENSOR FOR MONITORING OF THEIR DIFFERENTIATION STATUS

    Proceedings of the 72nd Chemical Sensor Symposium   39 ( Supplement A ) 58 - 60  2023.03

  • Application of Isothermal Nucleic Acid Amplification to Semiconductor Biosensor toward Highly Sensitive RNA Detection

    Proceedings of the 71st Chemical Sensor Symposium   38 ( Supplement B ) 25 - 27  2022.09

  • DETECTION OF 5-FLUOROURACIL IN SERUM BY SEMICONDUCTOR BIOSENSOR USING COMPETITION WITH PSEUDOANTIGEN

    Proceedings of the 70th Chemical Sensor Symposium   38 ( Supplement A ) 82 - 84  2022.03

  • Field-Effect Transistor Biosensor with Immobilized Cortisol Aptamer Based on Change of Charge Distribution with Transformation of Receptor Molecules

    黒岩繁樹, 林宏樹, 遠山良, 大橋啓之, 門間聰之, 門間聰之, 逢坂哲彌, 逢坂哲彌

    電気化学会大会講演要旨集(CD-ROM)   37 ( Supplement A ) 10 - 12  2021.03

    J-GLOBAL

  • Fabrication of aptamer-immobilized FET biosensor for sensitive detection of uncharged molecules using target-aptamer complex

    Proceedings of the 66th Chemical Sensor Symposium   66 ( Supplement B ) 97 - 99  2019.09

    CiNii

  • DETECTION OF SECRETORY IgA BY FIELD EFFECT TRANSISTOR BIOSENSOR USING JACALIN AS A SMALL RECEPTOR

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