Updated on 2025/09/03

写真a

 
HAYASHI, Hiroki
 
Affiliation
Faculty of Science and Engineering, School of Advanced Science and Engineering
Job title
Assistant Professor(non-tenure-track)
Degree
博士（工学） ( 2021.03 早稲田大学 )

Research Experience

  • 2023.04
    -
    Now

    Waseda University   Department of Applied Chemistry, School of Advanced Science and Engineering, Faculty of Science and Engineering   Assistant Professor

  • 2021.04
    -
    2023.03

    Waseda University   Department of Applied Chemistry, School of Advanced Science and Engineering   Assistant Professor

Education Background

  • 2018.04
    -
    2021.03

    Waseda University   Graduate School of Advanced Science and Engineering   Department of Nanoscience and Nanoengineering  

Committee Memberships

  • 2025.04
    -
    Now

    電気化学会  関東支部幹事

Professional Memberships

  • 2020.02
    -
    Now

    化学センサ研究会

  • 2018.08
    -
    Now

    電気化学会

Research Areas

  • Energy chemistry / Nanobioscience

Awards

  • 水野賞

    2021.03   早稲田大学   生体分子間相互作用に基づく分子認識界面により機能化した電界効果トランジスタバイオセンサ

  • 優秀賞および異分野賞

    2019.09   第2回COI学会   簡便なストレスマーカー検出のための半導体センサ界面の構築とバイオセンシングシステム

  • 優秀ポスター賞

    2018.10   第18回Conference for BioSignal and Medicine (CBSM)   インフルエンザウイルスの宿主域識別に向けた糖鎖固定化半導体センサの評価

  • Best Presentation Award

    2018.06   International Symposium on Biological Material Science for Agriculture and Engineering- Aiming at Future Interdisciplinary Collaborations   Discrimination of influenza virus subtypes in nasal mucus using glycan-immobilized field effect transistor biosensor

 

Papers

  • Site-specific aptamer immobilization via amine-to-thiol cross-linking on field-effect transistor biosensor for cortisol detection

    Hiroki Hayashi, Akane Ishikawa, Ayaka Osakada, Junichi Uchida, Toshiyuki Momma

    Colloids and Surfaces B: Biointerfaces   254   114842  2025.10  [Refereed]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

  • Mechanism of Lithium-Ion Extraction and Charge-Discharge Processes in LixNi1/3Mn1/3Co1/3O2 as Cathode for Alkaline Zinc Batteries

    Takahiro Kosaki, Hiroki Hayashi, Hiroki Nara, Gota Asano, Toshiyuki Momma

    Journal of The Electrochemical Society   172   060531  2025.06  [Refereed]

    Authorship：Corresponding author

    DOI

    Scopus

  • Artificial solid electrolyte interphase composed of a tris(2-acryloyloxyethyl) isocyanurate-based polymer for lithium metal anode

    Hiroki Nara, Takumi Miyamoto, Takahiro Kosaki, Hiroki Hayashi, Toshiyuki Momma

    EES Batteries    2025  [Refereed]

    　View Summary

    An artificial solid electrolyte interphase based on tris(2-acryloyloxyethyl) isocyanurate-based polymer effectively suppresses lithium dendrite growth and uncontrolled SEI formation, enabling prolonged cycling stability.

    DOI

  • Field-effect transistor biosensor with signal amplification by ternary initiation complexes for detection of wide-range RNA concentration

    Hiroki Hayashi, Akihiro Enami, Hiroto Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masayasu Kuwahara, Tetsuya Osaka, Toshiyuki Momma

    Talanta   273   125846  2024.06  [Refereed]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

    5
    Citation
    (Scopus)

  • Semiconductor-based biosensor exploiting competitive adsorption with charged pseudo-target molecules for monitoring 5-fluorouracil concentration in human serum

    Hiroki Hayashi, Mayuri Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masahisa Okada, Futoshi Shibasaki, Tetsuya Osaka, Toshiyuki Momma

    Sensors and Actuators B: Chemical   395   134495 - 134495  2023.11  [Refereed]

    Authorship：Lead author, Corresponding author

    DOI

    Scopus

    2
    Citation
    (Scopus)

  • A Non‐Destructive Electrical Assay of Stem Cell Differentiation Based on Semiconductor Biosensing

    Sho Hideshima, Hiroki Hayashi, Sayoko Saito, Hiroaki Tateno, Toshiyuki Momma, Tetsuya Osaka

    Analysis & Sensing   3 ( 2 ) e202200046  2022.10  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    3
    Citation
    (Scopus)

  • Improvement in long-term stability of field effect transistor biosensor in aqueous environments using a combination of silane and reduced graphene oxide coating

    Sho Hideshima, Hiroki Hayashi, Ryo Takeuchi, Shofarul Wustoni, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Tetsuya Osaka

    Microelectronic Engineering   264   111859 - 111859  2022.08  [Refereed]

    Authorship：Lead author

    DOI

    Scopus

    10
    Citation
    (Scopus)

  • Potassium-regulated Immobilization of Cortisol Aptamer for Field-effect Transistor Biosensor to Detect Changes in Charge Distribution with Aptamer Transformation

    S. Kuroiwa, H. Hayashi, R. Toyama, N. Kaneko, K. Horii, K. Ohashi, T. Momma, T. Osaka

    Chemistry Letters   50 ( 5 ) 892 - 895  2021.05  [Refereed]

    Authorship：Lead author

    　View Summary

    Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of a solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guanine-quadruplex during the immobilization of the aptamers in a highly concentrated solution of K+.

    DOI

    Scopus

    6
    Citation
    (Scopus)

  • Immobilization of Target-Bound Aptamer on Field Effect Transistor Biosensor to Improve Sensitivity for Detection of Uncharged Cortisol

    Hiroki Hayashi, Ryo Toyama, Ryota Takibuchi, Sho Hideshima, Shigeki Kuroiwa, Naoto Kaneko, Katsunori Horii, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

    ELECTROCHEMISTRY   89 ( 2 ) 134 - 137  2021  [Refereed]

    Authorship：Lead author

    　View Summary

    Field effect transistor (FET) biosensors are capable of detecting various biomolecules, although challenges remain in the detection of uncharged molecules. In this study, the detection of uncharged cortisol was demonstrated by interfacial design using a technique to immobilize target-bound aptamers. The target-bound aptamers, which formed a higher-order structure than target-unbound aptamers, expanded the distance between adjacent aptamers and reduced the steric hindrance to the conformational change. The density-controlled aptamers efficiently induced their conformational changes with the cortisol binding, which resulted in the improvement of the sensitivity of FET biosensors. (C) The Author(s) 2020. Published by ECSJ.

    DOI

    Scopus

    12
    Citation
    (Scopus)

  • Tetrameric jacalin as a receptor for field effect transistor biosensor to detect secretory IgA in human sweat

    Hiroki Hayashi, Naoki Sakamoto, Sho Hideshima, Yoshitaka Harada, Mika Tsuna, Shigeki Kuroiwa, Keishi Ohashi, Toshiyuki Momma, Tetsuya Osaka

    Journal of Electroanalytical Chemistry   873   114371  2020.09  [Refereed]

    Authorship：Lead author

    　View Summary

    Secretory immunoglobulin A (s-IgA), found in biological fluids, is useful for monitoring condition on mental health to prevent depression. In this study, the non-invasive detection of s-IgA in human sweat was demonstrated using field effect transistor (FFT) bioscnsors modified with a plant lectin, jacalin, as a receptor. The s-IgA molecules were detected with greater sensitivity using the jacalin-immobilized FET biosensors as compared to the sensitivity shown by Fabimmobilized FET bioscnsors. Jacalin, which is a small lectin tetramcr, has four glycan-binding sites and can capture a large number of s-IgA molecules within the charge-detectable region in terms of Dcbye length. Moreover, the jacalin-immobilized FET bioscnsor could detect s-IgA at concentrations ranging from 0.1 mu g/ml. to 100 mu g/mL Additionally, by using a filtration process to eliminate the interference of other components found in human sweat, our FET sensing system could specifically and quantitatively detect s-IgA. Therefore, our results show the utility of this device in monitoring mental stress.

    DOI

    Scopus

    13
    Citation
    (Scopus)

  • Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles

    Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka

    Scientific Reports   9   11616  2019.08  [Refereed]

    　View Summary

    Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.

    DOI

    Scopus

    37
    Citation
    (Scopus)

  • Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor

    S. Hideshima, S. Wustoni, M. Kobayashi, H. Hayashi, S. Kuroiwa, T. Nakanishi, T. Osaka

    Sensing and Bio-Sensing Research   17   25 - 29  2018.02  [Refereed]

    DOI

    Scopus

    17
    Citation
    (Scopus)

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Presentations

  • コルチゾール検出に向けた半導体バイオセンサにおけるジスルフィド結合を利用したアプタマー固定化法

    林宏樹, 門間聰之, 内田淳一

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第13回研究交流フォーラム 

    Presentation date： 2025.07

  • 多数のDNAzymを有する増幅核酸鎖によるRNA検出における電気化学測定法比較

    林宏樹, 佐瀬弘, 門間聰之, 桒原正靖

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第13回研究交流フォーラム 

    Presentation date： 2025.07

  • Aptamer Immobilization on Transistor-Based Biosensor Via Crosslinker for Amine-to-Thiol Conjugation Toward Cortisol Detection

    H. Hayashi, A. Ishikawa, T. Momma, J. Uchida

    Pacific Rim Meeting on Electrochemical and Solid-State Science 2024 (PRiME 2024) 

    Presentation date： 2024.10

    Event date：
    2024.10
    　
    　

  • Interfacial Design of Semiconductor-based Biosensor for Biomarker Detection

    Hiroki Hayashi  [Invited]

    13th Jilin-Korea-Waseda Alliance Annual Symposium 

    Presentation date： 2024.08

  • バイオマーカー検出に向けた半導体型バイオセンサの界面設計と開発

    林宏樹

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第12回研究交流フォーラム 

    Presentation date： 2024.07

  • 亜鉛二次電池用正極への適用に向けた NMC111の脱リチウム処理の挙動

    林宏樹, 甲崎孝裕, 奈良洋希, 浅野剛太, 門間聰之

    電気化学会 第91回大会 

    Presentation date： 2024.03

    Event date：
    2024.03
    　
    　

  • Highly sensitive RNA detection using field effect transistor biosensor with isothermal nucleic acid amplification

    Hiroki Hayashi, Akihiro Enami, Hiroto Fujita, Shigeki Kuroiwa, Keishi Ohashi, Masayasu Kuwahara, Toshiyuki Momma, Tetsuya Osaka

    33rd Anniversary World Congress on Biosensors 2023 

    Presentation date： 2023.06

    Event date：
    2023.06
    　
    　

  • Design of semiconductor-based biosensing system for uncharged molecule detection

    Hiroki Hayashi  [Invited]

    8th DGIST-Waseda Workshop on Electrochemistry 2022 

    Presentation date： 2022.11

  • 水系亜鉛負極電池用正極への三元系層状化合物の適用

    林宏樹, 浅野剛太, 三栗谷仁, 門間聰之, 逢坂哲彌

    第63回電池討論会 

    Presentation date： 2022.11

    Event date：
    2022.11
    　
    　

  • 電界効果トランジスタバイオセンサを用いた抗がん剤５-フルオロウラシルの検出

    林宏樹, 黒岩繁樹, 大橋啓之, 門間聰之, 逢坂哲彌, 岡田政久, 芝崎太

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第10回研究交流フォーラム 

    Presentation date： 2022.06

  • Detection of 5-Fluorouracil in Serum by Semiconductor Biosensor Using Competition with Pseudoantigen

    Presentation date： 2022.03

    Event date：
    2022.03
    　
    　

  • Detection of Stress-Related Secretory IgA in Human Sweat Using Lectin-Immobilized Field Effect Transistor Biosensor

    H. Hayashi, N. Sakamoto, S. Hideshima, Y. Harada, M. Tsuna, S. Kuroiwa, K. Ohashi, T. Momma, T. Osaka

    Pacific Rim Meeting on Electrochemical and Solid-State Science 2020 (PRiME 2020) 

    Presentation date： 2020.10

  • 簡便なストレスマーカー検出のための半導体センサ界面の構築とバイオセンシングシステム

    林宏樹

    第2回COI学会  (東京) 

    Presentation date： 2019.09

  • Functionalization of Semiconductor-Based Biosensor by Glycan toward the Detection of Influenza Virus

    H. Hayashi

    NIMS-Waseda Joint Symposium  (東京) 

    Presentation date： 2019.07

  • Semiconductor-Based Portable Biosensor for Food Allergen Detection

    S. Hideshima, S. Kuroiwa, H. Hayashi, Y. Harada, M. Tsuna, T. Momma, T. Osaka

    The IEEE International Symposium on Circuits and Systems 2019 (ISCAS2019)  (北海道) 

    Presentation date： 2019.06

  • Discrimination of Influenza virus subtypes in mucus samples using glycan-immobilized semiconductor-based biosensor

    H. Hayashi, T. Momma, T. Osaka

    6th DGIST-Waseda Workshop on Electrochemistry 2018  (大邱) 

    Presentation date： 2018.11

  • インフルエンザウイルスの宿主域識別に向けた糖鎖固定化半導体センサの評価

    林宏樹, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 門間聰之, 逢坂哲彌

    第18回Conference for BioSignal and Medicine (CBSM)  (神奈川) 

    Presentation date： 2018.10

  • Detection of Whole Influenza Viral Particle in High Ionic Strength Solution by using Glycan-Immobilized Field Effect Transistor Biosensor

    S. Hideshima, H. Hayashi, S. Kuroiwa, T. Osaka

    The 12th International Symposium on Electrochemical Micro & Nano System Technologies (EMNT2018)  (ミラノ) 

    Presentation date： 2018.09

  • Detection of Influenza virus in nasal mucus by viscosity reduction using glycan-immoobilized FET biosensor

    H. Hayashi, S. Hideshima, H. Hinou, SI. Nishimura, Y. Sakoda, S. Kuroiwa, T. Nakanishi, T. Momma, T. Osaka

    28th Anniversary World Congress on Biosensors 2018  (フロリダ) 

    Presentation date： 2018.06

  • Discrimination of influenza virus subtypes in nasal mucus using glycan-immobilized field effect transistor biosensor

    H. Hayashi, S. Hideshima, S. Kuroiwa, T. Momma, T. Osaka

    International Symposium on Biological Material Science for Agriculture and Engineering- Aiming at Future Interdisciplinary Collaborations  (東京) 

    Presentation date： 2018.06

  • 電界効果トランジスタバイオセンサを用いたアミロイドβ凝集体の検出

    林宏樹, 秀島翔, 黒岩繁樹, 大橋啓之, 門間聰之, 逢坂哲彌

    東京バイオマーカー・イノベーション技術研究組合（TOBIRA） 第7回研究交流フォーラム  (東京) 

    Presentation date： 2018.05

  • Development of glycan-immobilized FET biosensor toward the detection of Influenza virus from biological sample

    H. Hayashi, S. Hideshima, S. Kuroiwa, T. Nakanishi, T. Momma, T. Osaka

    5th DGIST-Waseda Workshop on Electrochemistry 2017  (東京) 

    Presentation date： 2017.12

  • 糖鎖固定化電界効果トランジスタバイオセンサによるインフルエンザウイルス粒子の高感度検出

    林宏樹, 秀島翔, 比能洋, 西村紳一郎, 迫田義博, 黒岩繁樹, 中西卓也, 門間聰之, 逢坂哲彌

    Conference for BioSignal and Medicine (CBSM) 第16回大会  (大分) 

    Presentation date： 2016.09

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Industrial Property Rights

  • 小型非荷電分子の検出方法

    特許第7709140号

    逢坂哲彌, 門間聰之, 大橋啓之, 黒岩繁樹, 林宏樹, 芝崎太

    Patent

  • 非荷電分子の検出方法及びプローブ分子固定化半導体センシングデバイス

    門間聰之, 林宏樹, 内田淳一, 萬隆行

    Patent

  • 亜鉛２次電池の正極活物質、亜鉛２次電池、および、亜鉛２次電池の製造方法

    逢坂哲彌, 門間聰之, 三栗谷仁, 林宏樹, 浅野剛太

    Patent

  • アプタマー固定化半導体センシングデバイス及び非荷電分子の検出方法

    特許第7276774号

    逢坂 哲彌, 大橋 啓之, 黒岩 繁樹, 林 宏樹

    Patent

  • レクチン固定化半導体センシングデバイス及び糖化合物の検出方法

    特許第7268411号

    逢坂哲彌, 門間聰之, 秀島翔, 林宏樹, 綱美香, 原田義孝

    Patent

  • アプタマー固定化半導体センシングデバイス及び非荷電分子の検出方法

    逢坂哲彌, 大橋啓之, 黒岩繁樹, 林宏樹

    Patent

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Syllabus

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