Updated on 2024/02/29

写真a

 
SUGIYAMA, Kaori
 
Affiliation
Faculty of Science and Engineering, Waseda Research Institute for Science and Engineering
Job title
Junior Researcher(Assistant Professor)
Degree
博士(人間生物学) ( 筑波大学 )

Research Experience

  • 2020.09
    -
    Now

    Waseda University   Faculty of Science and Engineering   Junior Researcher / Assistant Professor

  • 2020.06
    -
    2020.08

    University of Tsukuba   Life Science Center of Tsukuba Advanced Research Alliance (TARA)   Posdoc

  • 2017.02
    -
    2018.03

    Fraunhofer IGB / University of Tübingen   visiting reesarcher

Education Background

  • 2015.04
    -
    2020.05

    University of Tsukuba   Ph.D. Program in Human Biology   Ph.D. Program in Human Biology  

Professional Memberships

  •  
    -
    Now

    日本分光学会

  •  
     
     

    AHA

  •  
     
     

    日本血管生物医学会

  •  
     
     

    日本分子生物学会

  •  
     
     

    日本化学会

  •  
     
     

    日本結合組織学会

▼display all

Research Areas

  • Medical biochemistry / Applied biochemistry / Biomedical engineering / Pathological biochemistry

Research Interests

  • Photochemistry

  • Vascular biology

  • Cardiovascular disease

  • Multivariate data analysis

  • Label-free imaging

  • Raman microspectroscopy

  • Extracellular matrix

  • Thoracic aortic aneurysm

▼display all

Awards

  • 若手優秀賞YIA

    2023.12   日本血管生物医学会  

  • コニカミノルタ画像科学奨励賞

    2023.03   コニカミノルタ科学技術振興財団  

  • 井上研究奨励賞

    2023.02   井上科学振興財団  

  • Best Speaker Award

    2018.09   Tsukuba Global Science Week 2018  

  • International Travel Award

    2018.07  

  • Young Investigator Award

    2018.07  

  • First Prize

    2018.06   Annual Conference of Advanced Applied Raman Spectroscopy  

  • Poster Award

    2018.03   International Joint Meeting of the German and Swiss Societies of Matrix Biology  

  • Best Improve Award

    2015.09   Edinburgh Summer School  

  • ポスター賞

    2014.12   日本化学会関東支部群馬地区地域懇親会  

▼display all

 

Papers

  • Single-Cell Level Raman Molecular Profiling Reveals the Classification of Growth Phases of Chaetoceros tenuissimus

    Masahiro Ando, Kaori Sugiyama, Koya Kubo, Shumpei Horii, Takeshi Hano, Yuji Tomaru, Haruko Takeyama

    The Journal of Physical Chemistry B   127 ( 22 ) 5027 - 5033  2023.05  [Refereed]

    Authorship:Lead author

    DOI

    Scopus

  • Protective Role of Endothelial Fibulin‐4 in Valvulo‐Arterial Integrity

    Tram Anh Vu Nguyen, Caroline Antunes Lino, Huynh Thuy Hang, Juliano Vilela Alves, Bui Quoc Thang, Seung Jae Shin, Kaori Sugiyama, Hiroko Matsunaga, Haruko Takeyama, Yoshito Yamashiro, Hiromi Yanagisawa

    Journal of the American Heart Association   12 ( 1 )  2023.01  [Refereed]

     View Summary

    Background

    <p lang="en"> Homeostasis of the vessel wall is cooperatively maintained by endothelial cells (ECs), smooth muscle cells, and adventitial fibroblasts. The genetic deletion of fibulin‐4 ( Fbln4 ) in smooth muscle cells ( SMKO ) leads to the formation of thoracic aortic aneurysms with the disruption of elastic fibers. Although Fbln4 is expressed in the entire vessel wall, its function in ECs and relevance to the maintenance of valvulo‐arterial integrity are not fully understood.

    </p> Methods and Results

    <p lang="en"> Gene silencing of FBLN4 was conducted on human aortic ECs to evaluate morphological changes and gene expression profile. Fbln4 double knockout ( DKO ) mice in ECs and smooth muscle cells were generated and subjected to histological analysis, echocardiography, Western blotting, RNA sequencing, and immunostaining. An evaluation of the thoracic aortic aneurysm phenotype and screening of altered signaling pathways were performed. Knockdown of FBLN4 in human aortic ECs induced mesenchymal cell–like changes with the upregulation of mesenchymal genes, including TAGLN and MYL9 . DKO mice showed the exacerbation of thoracic aortic aneurysms when compared with those of SMKO and upregulated Thbs1, a mechanical stress–responsive molecule, throughout the aorta. DKO mice also showed progressive aortic valve thickening with collagen deposition from postnatal day 14, as well as turbulent flow in the ascending aorta. Furthermore, RNA sequencing and immunostaining of the aortic valve revealed the upregulation of genes involved in endothelial‐to‐mesenchymal transition, inflammatory response, and tissue fibrosis in DKO valves and the presence of activated valve interstitial cells.

    </p> Conclusions

    <p lang="en">The current study uncovers the pivotal role of endothelial fibulin‐4 in the maintenance of valvulo‐arterial integrity, which influences thoracic aortic aneurysm progression.

    </p>

    DOI

  • Direct intracellular detection of biomolecule specific bound-water with Raman spectroscopy

    Ashok Zachariah Samuel, Kaori Sugiyama, Haruko Takeyama

    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy     121870 - 121870  2023.01  [Refereed]

    DOI

    Scopus

    3
    Citation
    (Scopus)
  • Direct imaging of intracellular RNA, DNA, and liquid–liquid phase separated membraneless organelles with Raman microspectroscopy

    Ashok Zachariah Samuel, Kaori Sugiyama, Masahiro Ando, Haruko Takeyama

    Communications Biology   5 ( 1 )  2022.12  [Refereed]

     View Summary

    Abstract

    Methodologies for direct intracellular imaging of RNA and DNA are necessary for the advancement of bioimaging. Here we show direct label-free imaging of RNA and DNA in single cells by isolating their accurate Raman spectra. Raman images of DNA from interphase cells show intact nucleus, while those from mitotic cells reveal condensed chromosome. The condensed chromosome images are accurate enough to assign the stage of mitotic cell division (e.g., metaphase). Raman spectral features indicate B-DNA double helical conformational form in all the cell lines investigated here. The Raman images of RNAs, on the other hand, reveal liquid-liquid phase separated (LLPS) membraneless organelles in interphase cells, which disappears during mitosis. Further, the Raman spectrum of proteins from the intracellular LLPS organelles indicates slight enrichment of amyloid-like secondary structural features. Vibrational imaging of intracellular DNA and RNA simultaneously would open myriad of opportunities for examining functional biochemical aspects of cells and organelles.

    DOI

    Scopus

  • Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms

    Kaori Sugiyama, Julia Marzi, Julia Alber, Eva M. Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Cell Reports Medicine     100261 - 100261  2021.04  [Refereed]

    Authorship:Lead author

    DOI

    Scopus

    14
    Citation
    (Scopus)
  • Novel ELN mutation in a Japanese family with a severe form of supravalvular aortic stenosis.

    Kaori Sugiyama, Hitoshi Horigome, Lisheng Lin, Takashi Murakami, Junko Shiono, Yoshito Yamashiro, Hiroyuki Matsuura, Hitoshi Yoda, Hiromi Yanagisawa

    Molecular genetics & genomic medicine   7 ( 11 ) e986  2019.11  [Refereed]  [International journal]

    Authorship:Lead author

     View Summary

    BACKGROUND: Supravalvular aortic stenosis (SVAS) is one of the congenital cardiovascular diseases characterized by stenosis of the aorta. The stenotic lesions occur anywhere above the aortic valve in the aortic tree as well as pulmonary arteries and eventually leads to circulatory failure. The disease gene has been identified on the elastin gene (ELN) and two types of SVAS have been categorized; a familial type and an isolated type with the de novo mutation. METHODS: Fluorescent In situ hybridization (FISH) analysis and gene sequencing were performed in a two-generation family in which severe form of SVAS was diagnosed. RESULTS: None of the patients tested showed microdeletion of ELN, LIMK1, and D7S613. A novel nonsense mutation of ELN (c.160G>T (p.(Gly54*)), RNA not analyzed) was found in exon 3 in three members; two of them died suddenly due to rapid progression of SVAS with possible arrhythmia in early infancy. A point mutation in the 5' untranslated region, which was previously suggested to be associated with SVAS, did not co-segregate with the SVAS phenotype and found to be SNPs. CONCLUSION: Our report shows a broad spectrum of clinical features in family members sharing the identical mutations, suggesting a potential contribution of modifier gene(s) or interactions with environmental factors.

    DOI PubMed

    Scopus

    2
    Citation
    (Scopus)
  • 家族性大動脈弁上狭窄症における新規エラスチン遺伝子変異の同定と解析

    杉山 夏緒里, 堀米 仁志, 村上 卓, 林 立申, 野崎 良寛, 塩野 淳子, 松裏 裕行, 緒方 公平, 柳沢 裕美

    日本小児科学会雑誌   123 ( 2 ) 346 - 346  2019.02

  • Role of Thrombospondin-1 in Mechanotransduction and Development of Thoracic Aortic Aneurysm in Mouse and Humans.

    Yoshito Yamashiro, Bui Quoc Thang, Seung Jae Shin, Caroline Antunes Lino, Tomoyuki Nakamura, Jungsil Kim, Kaori Sugiyama, Chiho Tokunaga, Hiroaki Sakamoto, Motoo Osaka, Elaine C Davis, Jessica E Wagenseil, Yuji Hiramatsu, Hiromi Yanagisawa

    Circulation research   123 ( 6 ) 660 - 672  2018.08  [Refereed]  [International journal]

     View Summary

    RATIONALE: Abnormal mechanosensing of smooth muscle cells (SMCs) resulting from the defective elastin-contractile units has been suggested to drive the formation of thoracic aortic aneurysms; however, the precise molecular mechanism has not been elucidated. OBJECTIVE: The aim of this study was to identify the crucial mediator(s) involved in abnormal mechanosensing and propagation of biochemical signals during the aneurysm formation and to establish a basis for a novel therapeutic strategy. METHODS AND RESULTS: We used a mouse model of postnatal ascending aortic aneurysms ( Fbln4SMKO; termed SMKO [SMC-specific knockout]), in which deletion of Fbln4 (fibulin-4) leads to disruption of the elastin-contractile units caused by a loss of elastic lamina-SMC connections. In this mouse, upregulation of Egr1 (early growth response 1) and angiotensin-converting enzyme leads to activation of Ang II (angiotensin II) signaling. Here, we showed that the matricellular protein, Thbs1 (thrombospondin-1), was highly upregulated in SMKO ascending aortas and in human thoracic aortic aneurysms. Thbs1 was induced by mechanical stretch and Ang II in SMCs, for which Egr1 was required, and reduction of Fbln4 sensitized the cells to these stimuli and led to higher expression of Egr1 and Thbs1. Deletion of Thbs1 in SMKO mice prevented the aneurysm formation in ≈80% of DKO (SMKO;Thbs1 knockout) animals and suppressed Ssh1 (slingshot-1) and cofilin dephosphorylation, leading to the formation of normal actin filaments. Furthermore, elastic lamina-SMC connections were restored in DKO aortas, and mechanical testing showed that structural and material properties of DKO aortas were markedly improved. CONCLUSIONS: Thbs1 is a critical component of mechanotransduction, as well as a modulator of elastic fiber organization. Maladaptive upregulation of Thbs1 results in disruption of elastin-contractile units and dysregulation of actin cytoskeletal remodeling, contributing to the development of ascending aortic aneurysms in vivo. Thbs1 may serve as a potential therapeutic target for treating thoracic aortic aneurysms.

    DOI PubMed

    Scopus

    42
    Citation
    (Scopus)
  • Stimulation of Elastic Fiber Proteins by Mesenchymal Stem Cell-Derived Factors

    Sides RE, Sugiyama K, Ramaswamy AK, Vorp DA, Yanagisawa H, Weinbaum JS

    Ingenium Journal of Undergraduate Research     76 - 81  2018  [Refereed]  [International journal]

  • Investigation of the mechanism of photochemically-induced lysozyme crystallization

    Tetsuo Okutsu, Tohru Taguchi, Jyunya Korenaga, Takashi Kuroiwa, Yu Ishikawa, Shiori Iizuka, Kaori Sugiyama, Hiroaki Horiuchi, Hiroshi Hiratsuka

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY   321   180 - 186  2016.05  [Refereed]

     View Summary

    This work examined the mechanism involved in photoinduced crystallization of a lysozyme, focusing on the structure of the photoproducts that lead to crystal growth. It was determined that dimers formed via linking of tryptophan (Trp) residues are preferentially produced when applying a high-excitation photon fluence. However, these dimers do not lead to crystal growth. A low fluence forms dimers via the combination of tyrosine (Tyr) residues, generating six types of Tyr-Tyr dimers. The dimer that originates from the linking of Tyr(53) and Tyr(53) has a configuration similar to that of the two adjacent, yet non-linked, lysozyme molecules in the crystal unit cell. The Tyr(53)-Tyr(53) dimer hence acts as a template for lysozyme crystal nucleation. (C) 2016 Elsevier B.V. All rights reserved.

    DOI

    Scopus

    1
    Citation
    (Scopus)

▼display all

Presentations

  • ラマンイメージングによる胸部大動脈瘤新規バイオマーカーの同定

    杉山夏緒里  [Invited]

    レーザー学会学術講演会第44回年次大会 

    Presentation date: 2024.01

  • マルファン症候群モデルマウスにおける急性大動脈解離因子の探索

    杉山夏緒里

    第31回日本血管生物医学会学術集会 

    Presentation date: 2023.12

  • Unraveling Raman Biomarkers in Human Skin Stem Cells

    Kaori Sugiyama, Masahiro Ando, Aiko Sada, Haruko Takeyama

    Presentation date: 2023.12

  • ヒト皮膚を用いたラマンイメージングによる表皮幹細胞シート評価を目指す新規バイオマーカーの探索

    杉山夏緒里, 安藤正浩, 佐田亜衣子, 竹山春子

    第3回細胞シート工学イノベーションフォーラム 

    Presentation date: 2023.11

  • ラマンイメージングを用いたヒト表皮幹細胞新規バイオマーカーの探索

    杉山夏緒里, 安藤正浩, 佐田亜衣子, 竹山春子

    第7回エラスチン・関連分子研究会学術集会 

    Presentation date: 2023.11

  • マルファン症候群モデルマウスを用いた急性大動脈解離因子の探索

    杉山夏緒里, 安藤正浩, 松永浩子, 木村健一, 内野春希, 有田誠, Katja Schenke-Layland, 柳沢裕美, 竹山春子

    第17回メタボロームシンポジウム 

    Presentation date: 2023.10

  • Multimodal Imaging to Identify Pre-signals for Acute Aortic Dissection by Marfan Syndrome Model Mouse

    Kaori Sugiyama, Masahiro Ando, Hiroko Matsunaga, Haruki Uchino, Kenichi Kimura, Francesco Ramirez, Katja Schenke-Layland, Makoto Arita, Hiromi Yanagisawa, Haruko Takeyama

    Basic Cardiovascular Science 2023 

    Presentation date: 2023.08

    Event date:
    2023.07
    -
    2023.08
  • Multimodal Imaging to Identify Pre-signals for Acute Aortic Dissection by Marfan Syndrome Model Mouse

    Kaori Sugiyama, Masahiro Ando, Hiroko MAtsunaga, Haruki Uchino, Kenichi Kimura, Francesco Ramirez, Katja Schenke-Layland, Makoto Arita, Hiromi Yanagisawa, Haruko Takeyama

    Elastin, Elastic Fibers, and Microfibrils. Gordon Research Conference, Elastin and 

    Presentation date: 2023.07

    Event date:
    2023.07
     
     
  • Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms

     [Invited]

    Presentation date: 2023.06

  • ラベルフリーラマンイメージングによるマルファン症候群モデルマウスを用いた急性大動脈解離シグナル探索

    杉山夏緒里  [Invited]

    日本分光学会生細胞分光部会 

    Presentation date: 2023.03

  • ラマンイメージングと多変量解析を用いた胸部大動脈瘤に特異的な新規バイオマーカーの同定

    杉山夏緒里

    第30回日本血管生物医学会学術集会 

    Presentation date: 2022.12

  • マルファン症候群モデルマウスを用いた急性大動脈解離シグナル探索とラベルフリーイメージング作製

    杉山夏緒里, 安藤正浩, 我妻竜太, 松永浩子, 柳沢裕美, 竹山春子

    第45回日本分子生物学会年会 

    Presentation date: 2022.12

  • マウス新生児心臓における空間的転写物発現の解析とその機能的役割

    松永浩子, 牧野祐樹, 我妻竜太, 杉山夏緒里, 山崎美輝, 細川正人, 竹山春子

    第45回日本分子生物学会年会 

    Presentation date: 2022.11

  • 歯肉組織に対するヒノキチオールの浸透性評価

    宅見信哉, 倉光祥平, 安藤正浩, 杉山夏緒里, 林忠絋, 竹山春子

    第65回秋季日本歯周病学会学術大会 

    Presentation date: 2022.09

    Event date:
    2022.09
     
     
  • Label-free imaging for acute aortic dissection by using Marfan syndrome model mouse

    Presentation date: 2022.09

  • Label-free imaging for acute aortic dissection by using Marfan syndrome model mouse

    Kaori Sugiyama, Masahiro Ando, Ryota Wagatsuma, Hiroko Matsunaga, Francesco Ramirez, Katja Schenke-Layland, Hiromi Yanagisawa, Haruko Takeyama

    11th International Symposium on Marfan, Loeys-Dietz, and Related Conditions 

    Presentation date: 2022.08

  • A Novel mouse model of aortic dissection caused by a point mutation in the hybrid domain of the fibrillin-1 gene

    Kimura, Kenichi, Motoyama, Eri, Ishii, Ryutaro, Sugiyama, Kaori, Yamashiro, Yoshito, Deleeuw, Violette, Sips, Patrick, Backer, Julie De, Mosquera, Laura Muiño, Sakai, Lynn Y, Kanki, Sachiko, Nakamura, Tomoyuki, Yanagisawa, Hiromi

    11th International Symposium on Marfan, Loeys-Dietz, and Related Conditions 

    Presentation date: 2022.08

  • マルファン症候群モデルマウスを用いた急性大動脈解離シグナル探索とラベルフリーイメージング作製

    杉山夏緒里, 安藤正浩, 我妻竜太, 松永浩子, 柳沢裕美, 竹山春子

    第54回 日本結合組織学会学術大会 

    Presentation date: 2022.06

  • Identification of collagen-derived biomolecular fingerprints specific for thoracic aortic aneurysms by Raman microspectroscopy and imaging

    Kaori Sugiyama, Julia Marzi, Masahiro Ando, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa, Haruko Takeyama

    Presentation date: 2022.05

  • 新規Fbn1遺伝子変異マウスを用いたマルファン症候群の大動脈解離発症機序の解明

    本山 絵理, 木村 健一, 石井 柳太郎, 杉山 夏緒里, 山城 義人, 神吉 佐智子, 柳沢 裕美

    第5回エラスチン・関連分子関連研究会学術集会 

    Presentation date: 2021.12

  • ラマン分光法と多変量解析を用いた胸部大動脈瘤ラベルフリーイメージングと疾患特異的分子指紋の同定

    杉山夏緒里, Julia Marzi, Eva Maria Brauchle, 安藤正浩, 山城義人, Bhama Remkhelawon, Katja Schenke-Layland, 柳沢裕美

    第94回日本生化学大会 

    Presentation date: 2021.11

    Event date:
    2021.11
     
     
  • Analysis of the regulation mechanism in a neonatal mouse cardiac function by tissue spatial transcriptomics

    Presentation date: 2021.11

  • 組織空間的な転写産物解析によるマウス心機能発現制御機構の解析

    松永浩子, 牧野祐樹, 杉山夏緒里, 我妻竜太, 山崎美輝, 鈴木直子, 濱口悠, 浜田道昭, 竹山春子

    第73回日本生物工学会大会 

    Presentation date: 2021.10

  • Identification of biomolecular fingerprints specific for thoracic aortic aneurysms by Raman microspectroscopy and imaging combined with multivariate data analysis

    Presentation date: 2021.09

  • 多くの患者集積が認められた大動脈弁上狭窄症の1家系 ~胎児心エコー診断を含めて~

    林 立申, 矢野悠介, 塩野淳子, 杉山夏緒里, 柳沢裕美, 堀米仁志

    第56回日本小児循環器学会 

    Presentation date: 2020.11

  • Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms

    Sugiyama Kaori, Ph, D, Marzi Julia, Ph, D, Brauchle Eva Maria, Ph, Ando Masahiro, Ph.D, Yamashiro Yoshito, Ph, D, Ramkhelawon Bhama, Ph, D, Schenke-Layla, Katja, Ph.D, Yanagisawa Hiromi, M.D., Ph.D

    2020 GenTAC Aortic Summit 

    Presentation date: 2020.10

  • マウス大動脈のin vivo マルチモーダル非線形光学イメージング

    赤坂駿介, 杉山夏緒里, 柳沢裕美, 加納英明

    第14回 分子科学討論会 

    Presentation date: 2020.09

  • Identification of biomolecular fingerprints specific for thoracic aortic aneurysms by Raman microspectroscopy and imaging combined with multivariate data analysis

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Remkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Presentation date: 2020.03

  • マウス⼤動脈瘤のin vivoマルチモーダル⾮線形⼯学イメージング

    赤坂駿介, 杉山夏緒里, 柳沢裕美, 加納英明

    ⽇本分光学会⽣細胞分光部会シンポジウム 

    Presentation date: 2019.12

  • Label-free Raman imaging for non-invasive diagnosis tool in thoracic aortic aneurysm

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Presentation date: 2019.12

  • ラマン分光法と多変量解析を⽤いた胸部⼤動脈瘤に特異的な⽣体分⼦指紋の同定

    杉山夏緒里, Julia Marzi, Eva Baria Brauchle, 安藤正浩, 山城義人, Bhama Ramkhelawon, Katja Schenke-Layland, 柳沢裕美

    第4回 エラスチン・関連分⼦学術集会 

    Presentation date: 2019.12

  • Label-free Raman imaging for non-invasive diagnosis tool in thoracic aortic aneurysm

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Katja Schenke-Layland, Hiromi Yanagisawa

    The Gordon Research Seminar on Elastin, Elastic Fibers and Microfibrils 

    Presentation date: 2019.07

  • Label-free Raman imaging for non-invasive diagnosis tool in thoracic aortic aneurysm

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Katja Schenke-Layland, Hiromi Yanagisawa

    The Gordon Research Conference on Elastin, Elastic Fibers and Microfibrils 

    Presentation date: 2019.07

  • 家族性⼤動脈弁上狭窄症における新規エラスチン遺伝⼦変異の同定と解析

    杉山夏緒里, 堀米仁志, 村上 卓, 林, 野崎良寛, 塩野淳子, 松裏裕行, 緒方公平, 与田仁志, 柳沢裕美

    第122回⽇本⼩児学会学術集会 

    Presentation date: 2019.04

  • ラベルフリーラマンイメージング法による⼤動脈瘤疾患モデルマウスの弾性線維 と膠原線維の解析

    杉山夏緒里, Julia Marzi, Eva Baria Brauchle, 山城義人, Bhama Ramkhelawon, Katja Schenke-Layland, 柳沢裕美

    第3回 エラスチン・関連分⼦学術集会 

    Presentation date: 2018.12

  • Label-free Raman imaging of elastic fibers and collagen fibers in diseased and healthy mouse aortic tissues

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Yoshito Yamashiro, Katja Schenke-Layland, Hiromi Yanagisawa

    American Society for Matrix Biology Biannual Meeting 2018 

    Presentation date: 2018.10

  • Label-free Raman imaging of diseased and healthy mouse aortic tissues

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Tsukuba Global Science Week 

    Presentation date: 2018.09

  • Label-free Raman imaging of elastic fibers in disease and healthy mouse aortic tissues

    Kaori Sugiyama, Eva Maria Brauchle, Julia Marzi, Yoshito Yamashiro, Hiromi Yanagisawa, Katja Schenke-Layland

    Conference on advanced applied Raman spectroscopy Raman Fest 2018 

    Presentation date: 2018.06

  • Label-free Raman imaging of elastic fibers in disease and healthy mouse aortic tissues

    Kaori Sugiyama, Eva Maria Brauchle, Julia Marzi, Yoshito Yamashiro, Hiromi Yanagisawa, Katja Schenke-Layland

    Presentation date: 2018.06

  • Label-free Raman imaging of elastic fibers in disease and healthy mouse aortic tissues

    Kaori Sugiyama, Eva Maria Brauchle, Julia Marzi, Yoshito Yamashiro, Hiromi Yanagisawa, Katja Schenke-Layland

    International Joint Meeting of the German and Swiss Societies for Matrix Biology 

    Presentation date: 2018.03

  • Label-free Raman imaging of elastic fibers in disease and healthy mouse aortic tissues

    International Joint Meeting of the German and Swiss Societies for Matrix Biology, Stuttgart, Germany 

    Presentation date: 2018.03

  • Stimulation of Elastic Fiber Proteins by Mesenchymal Stem Cell-Derived Factors

    Sides RE, Sugiyama K, Ramaswamy AK, Vorp DA, Yanagisawa H, Weinbaum JS

    Pittsburgh Science 2017 Conference 

    Presentation date: 2017.10

  • Stimulation of Elastic Fiber Proteins by Mesenchymal Stem Cell-Derived Factors.

    Sides RE, Sugiyama K, Ramaswamy AK, Vorp DA, Yanagisawa H, Weinbaum JS

    2017 Meeting of Biomedical Engineering Society 

    Presentation date: 2017.10

  • 大動脈弁上狭窄症におけるエラスチン遺伝子変異の同定と解析

    杉山夏緒里, 山城義人, 堀米仁志, 柳沢裕美

    第1回エラスチン関連分子研究会 

    Presentation date: 2016.12

  • The photochemical reaction of amino acid residues that induces protein crystallization and search for template molecules

    Southern California System Biology Symposium 

    Presentation date: 2016.02

  • タンパク質の結晶化を誘起するアミノ酸残基の光化学反応とテンプレート分子の探求

    杉山夏緒里, 堀内宏明, 奥津哲夫

    第95回日本化学会春季年会 

    Presentation date: 2015.03

  • タンパク質の結晶化を誘起するアミノ酸残基の光化学反応とテンプレート分子の探求

    杉山夏緒里, 田口徹, 内海麻衣子, 高瀬裕太, 堀内宏明, 奥津哲夫

    生体調節研究所生命科学セミナー 

    Presentation date: 2014.12

  • タンパク質の結晶化を誘起するアミノ酸残基の光化学反応とテンプレート分子の探求

    杉山夏緒里, 田口徹, 内海麻衣子, 高瀬裕太, 堀内宏明, 奥津哲夫

    日本化学会関東支部群馬地区地域懇親会 

    Presentation date: 2014.12

  • タンパク質の結晶化を誘起するアミノ酸残基の光化学反応とテンプレート分子の探求

    Presentation date: 2014.11

▼display all

Research Projects

  • 内在性間葉系幹細胞を用いた変形性関節症治療のための再生医療基盤の開発

    日本医療研究開発機構(AMED)  再生・細胞医療・遺伝子治療実現加速化プログラム(再生・細胞医療・遺伝子治療研究開発課題(基礎応用研究課題)

    Project Year :

    2023.07
    -
    2026.03
     

    木村健一, 杉山夏緒里

  • ミクロフィブリル変異による大動脈解離の病態解明

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Project Year :

    2021.10
    -
    2025.03
     

  • マルチオミックス解析による大動脈瘤破裂の血管リモデリング機構解明

    日本学術振興会  科学研究費助成事業 若手研究

    Project Year :

    2021.04
    -
    2025.03
     

    杉山 夏緒里

     View Summary

    本研究は、大動脈瘤破裂モデルマウスにおいて、大動脈瘤破裂直前のシグナル探索のために、血管の位置ごとや継時変化による血管壁の構造変化を解明する。本年度は、野生型マウスと大動脈瘤破裂モデルマウスの薬剤投与群と非投与群について、組織学的な解析を行なった。組織学的解析から、それぞれの条件のマウスにおける血管壁構造の違いを同定した。ラマン顕微鏡による測定と、空間RNAseqを行う切片位置について、組織学的解析結果から検討を行なった。
    また、ラマン顕微鏡と空間RNAeqについてはそれぞれの大動脈組織に対する条件検討を行なった。ラマン顕微鏡における検討事項としては、レーザー強度、露光時間、解像度について行った。ラマンイメージングとスペクトル分解が行えるよなS/Nのよい最適な条件の検討を行なった。血管壁ごとの部位特異性を調べるため、広域条件や測定エリア数の検討を念入りに行なった。ラベルフリーラマンイメージングは、これまでの研究で同定した、弾性線維、膠原線維、アグリカン、ヴァーシカン、核について作製を行なった。空間RNAseqについては、微小組織における1細胞RNAseq解析のため、まずは血管1切片における条件と、レーザーマイクロダイセクションによる微小組織における条件の検討を行なった。レーザーマイクロダイセクションにおいては、どの程度微小なところまで切り出しが可能なのかの検討を行なった。微小組織取得後はRNAseqを行い、遺伝子発現情報が得られるかの検討を行なった。

  • ラマン分光法とリピドミクス解析による大動脈瘤破裂予測因子の探索

    公益財団法人 豊田理化学研究所  豊田理研スカラー

    Project Year :

    2023.04
    -
    2024.03
     

  • 組織の凹凸を保持した三次元皮膚モデルの構築と評価指標の確立

    AMED  再生医療実現拠点ネットワークプログラム(幹細胞・再生医学イノベーション創出プログラム)若手

    Project Year :

    2021.06
    -
    2024.03
     

    佐田亜衣子, 杉山夏緒里

Misc

  • ラマンイメージングと多変量解析による胸部大動脈瘤に特異的な分子指紋の同定

    杉山夏緒里

    分光研究   72 ( 1 ) 40 - 55  2023.04  [Refereed]

    Authorship:Lead author, Last author, Corresponding author

    Article, review, commentary, editorial, etc. (scientific journal)  

 

Syllabus

▼display all

Teaching Experience

  • Laboratory for Advanced Science and Engineering B

    Waseda University  

    2022.04
    -
    Now
     

  • Laboratory for Advanced Science and Engineering A

    Waseda University  

    2022.04
    -
    Now
     

  • Science and Engineering Laboratory

    Waseda University  

    2021.09
    -
    Now
     

  • Fundamental Bioscience Laboratory

    Waseda University  

    2021.04
    -
    Now
     

  • 理工学基礎実験1B Vブロック

    Waseda University  

    2020.09
    -
    Now
     

  • Current Topics in Bioscience

    Waseda University  

    2020.09
    -
    Now
     

  • Molecular Cell Biology A

    Waseda University  

    2020.09
    -
    Now
     

▼display all

 

Sub-affiliation

  • Faculty of Science and Engineering   School of Advanced Science and Engineering