SUGIYAMA, Kaori

写真a

Affiliation

Faculty of Science and Engineering, Waseda Research Institute for Science and Engineering

Job title

Junior Researcher(Assistant Professor)

Concurrent Post 【 display / non-display

  • Faculty of Science and Engineering   School of Advanced Science and Engineering

Research Institute 【 display / non-display

  • 2020
    -
    2022

    リサーチイノベ オープンイノベーション推進セクション   兼任センター員

Education 【 display / non-display

  • 2015.04
    -
    2020.05

    University of Tsukuba   Ph.D. Program in Human Biology   Ph.D. Program in Human Biology  

  • 2011.04
    -
    2015.03

    Gunma University   Faculty of Engineering   Department of Chemistry and Chemical Biology  

Degree 【 display / non-display

  • 筑波大学   博士(人間生物学)

Research Experience 【 display / non-display

  • 2020.09
    -
    Now

    Waseda University   Faculty of Science and Engineering   Junior Researcher / Assistant Professor

  • 2020.06
    -
    2020.08

    University of Tsukuba   Life Science Center of Tsukuba Advanced Research Alliance (TARA)   Posdoc

  • 2017.02
    -
    2018.03

    Fraunhofer IGB / University of Tübingen   visiting reesarcher

Professional Memberships 【 display / non-display

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    日本生化学会

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    日本循環器学会

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    日本化学会

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    American Society for Matrix Biology

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    日本結合組織学会

 

Research Areas 【 display / non-display

  • Medical biochemistry

  • Applied biochemistry

  • Biomedical engineering

  • Pathological biochemistry

Research Interests 【 display / non-display

  • Photochemistry

  • Vascular biology

  • Cardiovascular disease

  • Multivariate data analysis

  • Label-free imaging

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Papers 【 display / non-display

  • Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms

    Kaori Sugiyama, Julia Marzi, Julia Alber, Eva M. Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Cell Reports Medicine     100261 - 100261  2021.04  [Refereed]

    Authorship:Lead author

    DOI

  • Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    SSRN Electronic Journal    2020.06

    Authorship:Lead author

    DOI

  • Novel ELN mutation in a Japanese family with a severe form of supravalvular aortic stenosis.

    Kaori Sugiyama, Hitoshi Horigome, Lisheng Lin, Takashi Murakami, Junko Shiono, Yoshito Yamashiro, Hiroyuki Matsuura, Hitoshi Yoda, Hiromi Yanagisawa

    Molecular genetics & genomic medicine   7 ( 11 ) e986  2019.11  [Refereed]  [International journal]

    Authorship:Lead author

     View Summary

    BACKGROUND: Supravalvular aortic stenosis (SVAS) is one of the congenital cardiovascular diseases characterized by stenosis of the aorta. The stenotic lesions occur anywhere above the aortic valve in the aortic tree as well as pulmonary arteries and eventually leads to circulatory failure. The disease gene has been identified on the elastin gene (ELN) and two types of SVAS have been categorized; a familial type and an isolated type with the de novo mutation. METHODS: Fluorescent In situ hybridization (FISH) analysis and gene sequencing were performed in a two-generation family in which severe form of SVAS was diagnosed. RESULTS: None of the patients tested showed microdeletion of ELN, LIMK1, and D7S613. A novel nonsense mutation of ELN (c.160G>T (p.(Gly54*)), RNA not analyzed) was found in exon 3 in three members; two of them died suddenly due to rapid progression of SVAS with possible arrhythmia in early infancy. A point mutation in the 5' untranslated region, which was previously suggested to be associated with SVAS, did not co-segregate with the SVAS phenotype and found to be SNPs. CONCLUSION: Our report shows a broad spectrum of clinical features in family members sharing the identical mutations, suggesting a potential contribution of modifier gene(s) or interactions with environmental factors.

    DOI PubMed

  • Role of Thrombospondin-1 in Mechanotransduction and Development of Thoracic Aortic Aneurysm in Mouse and Humans.

    Yoshito Yamashiro, Bui Quoc Thang, Seung Jae Shin, Caroline Antunes Lino, Tomoyuki Nakamura, Jungsil Kim, Kaori Sugiyama, Chiho Tokunaga, Hiroaki Sakamoto, Motoo Osaka, Elaine C Davis, Jessica E Wagenseil, Yuji Hiramatsu, Hiromi Yanagisawa

    Circulation research   123 ( 6 ) 660 - 672  2018.08  [Refereed]  [International journal]

     View Summary

    RATIONALE: Abnormal mechanosensing of smooth muscle cells (SMCs) resulting from the defective elastin-contractile units has been suggested to drive the formation of thoracic aortic aneurysms; however, the precise molecular mechanism has not been elucidated. OBJECTIVE: The aim of this study was to identify the crucial mediator(s) involved in abnormal mechanosensing and propagation of biochemical signals during the aneurysm formation and to establish a basis for a novel therapeutic strategy. METHODS AND RESULTS: We used a mouse model of postnatal ascending aortic aneurysms ( Fbln4SMKO; termed SMKO [SMC-specific knockout]), in which deletion of Fbln4 (fibulin-4) leads to disruption of the elastin-contractile units caused by a loss of elastic lamina-SMC connections. In this mouse, upregulation of Egr1 (early growth response 1) and angiotensin-converting enzyme leads to activation of Ang II (angiotensin II) signaling. Here, we showed that the matricellular protein, Thbs1 (thrombospondin-1), was highly upregulated in SMKO ascending aortas and in human thoracic aortic aneurysms. Thbs1 was induced by mechanical stretch and Ang II in SMCs, for which Egr1 was required, and reduction of Fbln4 sensitized the cells to these stimuli and led to higher expression of Egr1 and Thbs1. Deletion of Thbs1 in SMKO mice prevented the aneurysm formation in ≈80% of DKO (SMKO;Thbs1 knockout) animals and suppressed Ssh1 (slingshot-1) and cofilin dephosphorylation, leading to the formation of normal actin filaments. Furthermore, elastic lamina-SMC connections were restored in DKO aortas, and mechanical testing showed that structural and material properties of DKO aortas were markedly improved. CONCLUSIONS: Thbs1 is a critical component of mechanotransduction, as well as a modulator of elastic fiber organization. Maladaptive upregulation of Thbs1 results in disruption of elastin-contractile units and dysregulation of actin cytoskeletal remodeling, contributing to the development of ascending aortic aneurysms in vivo. Thbs1 may serve as a potential therapeutic target for treating thoracic aortic aneurysms.

    DOI PubMed

  • Stimulation of Elastic Fiber Proteins by Mesenchymal Stem Cell-Derived Factors

    Sides RE, Sugiyama K, Ramaswamy AK, Vorp DA, Yanagisawa H, Weinbaum JS

    Ingenium Journal of Undergraduate Research     76 - 81  2018  [Refereed]  [International journal]

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Awards 【 display / non-display

  • Best Speaker Award

    2018.09   Tsukuba Global Science Week 2018  

  • International Travel Award

    2018.07  

  • Young Investigator Award

    2018.07  

  • First Prize

    2018.06   Annual Conference of Advanced Applied Raman Spectroscopy  

  • Poster Award

    2018.03   International Joint Meeting of the German and Swiss Societies of Matrix Biology  

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Research Projects 【 display / non-display

  • マルチオミックス解析による大動脈瘤破裂の血管リモデリング機構解明

    若手研究

    Project Year :

    2021.04
    -
    2024.03
     

    杉山 夏緒里

  • 組織の凹凸を保持した三次元皮膚モデルの構築と評価指標の確立

    Project Year :

    2021.06
    -
    2024.03
     

    佐田亜衣子, 杉山夏緒里

    Authorship: Coinvestigator(s)

Presentations 【 display / non-display

  • Raman Microspectroscopy and Imaging Reveal Novel Biomarkers Specific for Thoracic Aortic Aneurysms

    Sugiyama Kaori, Ph, D, Marzi Julia, Ph, D, Brauchle Eva Maria, Ph, Ando Masahiro, Ph.D, Yamashiro Yoshito, Ph, D, Ramkhelawon Bhama, Ph, D, Schenke-Layla, Katja, Ph.D, Yanagisawa Hiromi, M.D., Ph.D

    2020 GenTAC Aortic Summit 

    Presentation date: 2020.10

  • マウス大動脈のin vivo マルチモーダル非線形光学イメージング

    赤坂駿介, 杉山夏緒里, 柳沢裕美, 加納英明

    第14回 分子科学討論会 

    Presentation date: 2020.09

  • Identification of biomolecular fingerprints specific for thoracic aortic aneurysms by Raman microspectroscopy and imaging combined with multivariate data analysis

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Remkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Presentation date: 2020.03

  • マウス⼤動脈瘤のin vivoマルチモーダル⾮線形⼯学イメージング

    赤坂駿介, 杉山夏緒里, 柳沢裕美, 加納英明

    ⽇本分光学会⽣細胞分光部会シンポジウム 

    Presentation date: 2019.12

  • Label-free Raman imaging for non-invasive diagnosis tool in thoracic aortic aneurysm

    Kaori Sugiyama, Julia Marzi, Eva Maria Brauchle, Masahiro Ando, Yoshito Yamashiro, Bhama Ramkhelawon, Katja Schenke-Layland, Hiromi Yanagisawa

    Presentation date: 2019.12

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Syllabus 【 display / non-display

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Teaching Experience 【 display / non-display

  • Fundamental Bioscience Laboratory

    Waseda University  

    2021.04
    -
    Now
     

  • Current Topics in Bioscience

    Waseda University  

    2020.09
    -
    Now
     

  • Molecular Cell Biology A

    Waseda University  

    2020.09
    -
    Now
     

  • 理工学基礎実験1B Vブロック

    Waseda University  

    2020.09
    -
    2021.03