Updated on 2024/05/22

写真a

 
KATAOKA, Kosuke
 
Affiliation
Research Council (Research Organization), Comprehensive Research Organization
Job title
Researcher(Assistant Professor)
Degree
Doctor of Science ( 2020.07 Waseda University )

Research Experience

  • 2023.04
    -
    Now

    Waseda University   Comprehensive Research Organization

  • 2021.04
    -
    2023.03

    Waseda University   Comprehensive Research Organization   Junior Researcher (Assistant Professor)

  • 2021.10
    -
    2022.03

    Tokyo University of Agriculture and Technology

  • 2021.04
    -
    2021.09

    Nagahama Institute of Bio-Science and Technology   Lecturer (part-time)

  • 2020.09
    -
    2021.03

    Waseda University   Faculty of Science and Engineering   Assistant Professor (without Tenure)

  • 2018.04
    -
    2020.08

    Waseda university   Faculty of Science and Engineering   Research associate

▼display all

Education Background

  • 2016.04
    -
    2020.09

    Waseda university   Graduate School of Advanced Science and Engineering   Department of Life Science and Medical Bioscience  

  • 2014.04
    -
    2016.03

    Waseda university   Graduate School of Advanced Science and Engineering   Department of Life Science and Medical Bioscience  

  • 2010.04
    -
    2014.03

    Waseda university   School of Advanced Science and Engineering   Department of Life Science and Medical Bioscience  

Research Areas

  • Insect science / Genome biology / Neuroscience-general / Cell biology / Molecular biology

Awards

  • Demo Day Best Presentation Award

    2015.09   WASEDA-EDGE Program  

    Winner: Kosuke Kataoka

  • Award in JSN oral educational session for young investigators

    2015.09   The Japanese Society for Neurochemistry   Mitochondrial cereblon suppressed stress-induced cell death

    Winner: Kosuke Kataoka

 

Papers

  • DeepLabCut-based daily behavioural and posture analysis in a cricket

    Shota Hayakawa, Kosuke Kataoka, Masanobu Yamamoto, Toru Asahi, Takeshi Suzuki

    Biology Open    2024.03  [Refereed]

    Authorship:Corresponding author

     View Summary

    Circadian rhythms are indispensable intrinsic programs that regulate the daily rhythmicity of physiological processes, such as feeding and sleep. The cricket has been employed as a model organism for understanding the neural mechanisms underlying circadian rhythms in insects. However, previous studies measuring rhythm-controlled behaviours only analysed locomotive activity using seesaw-type and infrared sensor-based actometers. Meanwhile, advances in deep learning techniques have made it possible to analyse animal behaviour and posture using software that is devoid of human bias and does not require physical tagging of individual animals. Here, we present a system that can simultaneously quantify multiple behaviours in individual crickets, such as locomotor activity, feeding, and sleep-like states, in the long-term, using DeepLabCut, a supervised machine learning-based software for body keypoints labeling. Our system successfully labelled the six body parts of a single cricket with a high level of confidence and produced reliable data showing the diurnal rhythms of multiple behaviours. Our system also enabled the estimation of sleep-like states by focusing on posture, instead of immobility time, which is a conventional parameter. We anticipate that this system will provide an opportunity for simultaneous and automatic prediction of cricket behaviour and posture, facilitating the study of circadian rhythms.

    DOI

    Scopus

  • Complete mitochondrial genome sequences of two ground crickets, Dianemobius fascipes nigrofasciatus and Polionemobius taprobanensis (Orthoptera: Grylloidea: trigonidiidae)

    Kohyoh Murata, Kosuke Kataoka, Ryuto Sanno, Kazuhiro Satomura, Atsushi Ogura, Toru Asahi, Kei Yura, Takeshi Suzuki

    Mitochondrial DNA Part B   8 ( 12 ) 1311 - 1315  2023.12  [Refereed]

    DOI

    Scopus

  • Integrative single-cell RNA-seq analysis of vascularized cerebral organoids

    Yuya Sato, Toru Asahi, Kosuke Kataoka

    BMC Biology   21   245  2023.11  [Refereed]

    Authorship:Last author, Corresponding author

    DOI

    Scopus

  • Covalent Immobilization of Collagen Type I to a Polydimethylsiloxane Surface for Preventing Cell Detachment by Retaining Collagen Molecules under Uniaxial Cyclic Mechanical Stretching Stress

    Kazuaki Mori, Kosuke Kataoka, Yoshikatsu Akiyama, Toru Asahi

    Biomacromolecules    2023.10  [Refereed]

    Authorship:Corresponding author

    DOI

    Scopus

  • Spatial and Sexual Divergence of Gut Bacterial Communities in Field Cricket Teleogryllus occipitalis (Orthoptera: Gryllidae)

    Kazuya Hirata, Toru Asahi, Kosuke Kataoka

    Microbial Ecology    2023.07  [Refereed]

    Authorship:Last author, Corresponding author

     View Summary

    Abstract

    The insect gut is colonized by microbes that confer a myriad of beneficial services to the host, including nutritional support, immune enhancement, and even influence behavior. Insect gut microbes show dynamic changes due to the gut compartments, sex, and seasonal and geographic influences. Crickets are omnivorous hemimetabolous insects that have sex-specific roles, such as males producing chirping sounds for communication and exhibiting fighting behavior. However, limited information is available on their gut bacterial communities, hampering studies on functional compartmentalization of the gut and sex-specific roles of the gut microbiota in omnivorous insects. Here, we report a metagenomic analysis of the gut bacteriome of the field cricket Teleogryllus occipitalis using 16S rRNA V3-V4 amplicon sequencing to identify sex- and compartment-dependent influences on its diversity and function. The structure of the gut microbiota is strongly influenced by their gut compartments rather than sex. The species richness and diversity analyses revealed large difference in the bacterial communities between the gut compartments while minor differences were observed between the sexes. Analysis of relative abundance and predicted functions revealed that nitrogen- and oxygen-dependent metabolism and amino acid turnover were subjected to functional compartmentalization in the gut. Comparisons between the sexes revealed differences in the gut microbiota, reflecting efficiency in energy use, including glycolytic and carbohydrate metabolism, suggesting a possible involvement in egg production in females. This study provides insights into the gut compartment dependent and sex-specific roles of host-gut symbiont interactions in crickets and the industrial production of crickets.

    DOI

    Scopus

    1
    Citation
    (Scopus)
  • Genome and Genetic Engineering of the House Cricket (Acheta domesticus): A Resource for Sustainable Agriculture

    Aaron T. Dossey, Brenda Oppert, Fu-Chyun Chu, Marcé D. Lorenzen, Brian Scheffler, Sheron Simpson, Sergey Koren, J. Spencer Johnston, Kosuke Kataoka, Keigo Ide

    Biomolecules   13 ( 4 ) 589 - 589  2023.03  [Refereed]  [International coauthorship]

     View Summary

    Background: The house cricket, Acheta domesticus, is one of the most farmed insects worldwide and the foundation of an emerging industry using insects as a sustainable food source. Edible insects present a promising alternative for protein production amid a plethora of reports on climate change and biodiversity loss largely driven by agriculture. As with other crops, genetic resources are needed to improve crickets for food and other applications. Methods: We present the first high quality annotated genome assembly of A. domesticus from long read data and scaffolded to chromosome level, providing information needed for genetic manipulation. Results: Gene groups related to immunity were annotated and will be useful for improving value to insect farmers. Metagenome scaffolds in the A. domesticus assembly, including Invertebrate Iridescent Virus 6 (IIV6), were submitted as host-associated sequences. We demonstrate both CRISPR/Cas9-mediated knock-in and knock-out of A. domesticus and discuss implications for the food, pharmaceutical, and other industries. RNAi was demonstrated to disrupt the function of the vermilion eye-color gene producing a useful white-eye biomarker phenotype. Conclusions: We are utilizing these data to develop technologies for downstream commercial applications, including more nutritious and disease-resistant crickets, as well as lines producing valuable bioproducts, such as vaccines and antibiotics.

    DOI

    Scopus

    4
    Citation
    (Scopus)
  • An octopamine receptor involved in feeding behavior of the two-spotted spider mite, Tetranychus urticae Koch: a possible candidate for RNAi-based pest control

    Faten Abdelsalam Hamdi, Kosuke Kataoka, Yuka Arai, Naoki Takeda, Masanobu Yamamoto, Yasser F. O. Mohammad, Noureldin Abuelfadl Ghazy, Takeshi Suzuki

    Entomologia Generalis    2023.02  [Refereed]

    DOI

    Scopus

    5
    Citation
    (Scopus)
  • Dissecting Cricket Genomes for Advancement of Entomology and Entomophagy

    Kosuke Kataoka, Yuki Togawa, Ryuto Sanno, Toru Asahi, Kei Yura

    Biophysical Reviews    2022.01  [Refereed]

    Authorship:Lead author, Corresponding author

  • Comparative Analysis of Mitochondrial Genomes in Gryllidea (Insecta: Orthoptera): Implications for Adaptive Evolution in Ant-loving Crickets

    Ryuto Sanno, Kosuke Kataoka, Shota Hayakawa, Keigo Ide, Chuong N. Nguyen, Thao P. Nguyen, Binh T. N. Le, Oanh T. P. Kim, Katsuhiko Mineta, Haruko Takeyama, Makio Takeda, Toshiyuki Sato, Takeshi Suzuki, Kei Yura, Toru Asahi

    Genome Biology and Evolution   13 ( 10 )  2021.09  [Refereed]  [International journal]  [International coauthorship]

    Authorship:Lead author, Corresponding author

     View Summary

    Species of infraorder Gryllidea, or crickets, are useful invertebrate models for studying developmental biology and neuroscience. They have also attracted attention as alternative protein sources for human food and animal feed. Mitochondrial genomic information on related invertebrates, such as katydids, and locusts, has recently become available in attempt to clarify the controversial classification schemes, although robust phylogenetic relationships with emphasis on crickets remain elusive. Here, we report newly sequenced complete mitochondrial genomes of crickets to study their phylogeny, genomic rearrangements, and adaptive evolution. First, we conducted de novo assembly of mitochondrial genomes from eight cricket species and annotated protein-coding genes (PCGs) and transfer and ribosomal RNAs using automatic annotations and manual curation. Next, by combining newly described PCGs with public data of the complete Gryllidea genomes and gene annotations, we performed phylogenetic analysis and found gene order rearrangements in several branches. We further analyzed genetic signatures of selection in ant-loving crickets (Myrmecophilidae), which are small wingless crickets that inhabit ant nests. Three distinct approaches revealed two positively selected sites in the cox1 gene in these crickets. Protein 3D structural analyses suggested that these selected sites could influence the interaction of respiratory complex proteins, conferring benefits to ant-loving crickets with a unique ecological niche and morphology. These findings enhance our understanding of the genetic basis of cricket evolution without relying on estimates based on a limited number of molecular markers.

    DOI PubMed

    Scopus

    13
    Citation
    (Scopus)
  • Edible Wild Field Cricket (Brachytrupes portentosus) Trading in Bangladesh

    Md. Mehedi Hasan, Md. Mostafizur Rahman, Kosuke Kataoka, Kei Yura, Md. Omar Faruque, Faysal Rabby Shadhen, Md. Fuad Mondal

    Journal of Insects as Food and Feed    2021.06  [Refereed]  [International coauthorship]

  • Age-dependent Alteration in Mitochondrial Dynamics and Autophagy in Hippocampal Neuron of Cannabinoid CB1 Receptor-deficient Mice

    Kosuke Kataoka, Andras Bilkei-Gorzo, Chihiro Nozaki, Akinobu Togo, Keiichiro Nakamura, Keisuke Ohta, Andreas Zimmer, Toru Asahi

    Brain Research Bulletin   160   40 - 49  2020.07  [Refereed]  [International journal]  [International coauthorship]

    Authorship:Lead author, Corresponding author

     View Summary

    Endocannabinoid system activity contributes to the homeostatic defense against aging and thus may counteract the progression of brain aging. The cannabinoid type 1 (CB1) receptor activity declines with aging in the brain, which impairs neuronal network integrity and cognitive functions. However, the underlying mechanisms that link CB1 activity and memory decline remain unknown. Mitochondrial activity profoundly influences neuronal function, and age-dependent mitochondrial activity change is one of the known hallmarks of brain aging. As CB1 receptor is expressed on mitochondria and may regulate neuronal energy metabolism in hippocampus, we hypothesized that CB1 receptors might influence mitochondria in hippocampal neurons. Here, we found that CB1 receptor significantly affected mitochondrial autophagy (mitophagy) and morphology in an age-dependent manner. Serine 65-phosphorylated ubiquitin, a key marker for mitophagy, was reduced in adult CB1-deficient mice (CB1-KO) compared to those in wild type controls, particularly in CA1 pyramidal cell layer. Transmission electron microscopy (TEM) analysis showed reduced mitophagy-like events in hippocampus of adult CB1-KO. TEM analysis also showed that mitochondrial morphology in adult CB1-KO mice was altered shown by an increase in thin and elongated mitochondria in hippocampal neurons. 3D reconstruction of mitochondrial morphology after scanning electron microscopy additionally revealed an enhanced density of interconnected mitochondria. Altogether, these findings suggest that reduced CB1 signaling in CB1-KO mice leads to reduced mitophagy and abnormal mitochondrial morphology in hippocampal neurons during aging. These mitochondrial changes might be due to the impairments in mitochondrial quality control system, which links age-related decline in CB1 activity and impaired memory.

    DOI PubMed

    Scopus

    18
    Citation
    (Scopus)
  • The Draft Genome Dataset of the Asian Cricket Teleogryllus occipitalis for Molecular Research Toward Entomophagy

    Kosuke Kataoka, Ryuhei Minei, Keigo Ide, Atsushi Ogura, Haruko Takeyama, Makio Takeda, Takeshi Suzuki, Kei Yura, Toru Asahi

    Frontiers in Genetics   11  2020.05  [Refereed]

    Authorship:Lead author, Corresponding author

    DOI

    Scopus

    15
    Citation
    (Scopus)
  • Antioxidant activity of Ge-132, a synthetic organic germanium, on cultured mammalian cells

    Takeyoshi Wada, Takashi Hanyu, Kota Nozaki, Kosuke Kataoka, Tomoro Kawatani, Toru Asahi, Naoya Sawamura

    Biological and Pharmaceutical Bulletin   41 ( 5 ) 749 - 753  2018  [Refereed]

     View Summary

    Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

    DOI

    Scopus

    25
    Citation
    (Scopus)
  • Mitochondrial Cereblon Functions as a Lon-type Protease

    Kosuke Kataoka, China Nakamura, Toru Asahi, Naoya Sawamura

    Scientific Reports   6   29986  2016.07  [Refereed]

    Authorship:Lead author

     View Summary

    Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix, and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. However, whether CRBN has Lon protease-like function remains unknown. Here, we determined if CRBN has a protective function against oxidative stress, similar to Lon protease. We report that CRBN partially distributes in mitochondria, suggesting it has a mitochondrial function. To specify the mitochondrial role of CRBN, we mitochondrially expressed CRBN in human neuroblastoma SH-SY5Y cells. The resulting stable SH-SY5Y cell line showed no apparent effect on the mitochondrial functions of fusion, fission, and membrane potential. However, mitochondrially expressed CRBN exhibited protease activity, and was induced by oxidative stress. In addition, stably expressed cells exhibited suppressed neuronal cell death induced by hydrogen peroxide. These results suggest that CRBN functions specifically as a Lon-type protease in mitochondria.

    DOI

    Scopus

    15
    Citation
    (Scopus)

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Books and Other Publications

  • 最先端コオロギ学 ~世界初! 新しい生物学がここにある~

    野地澄晴( Part: Joint editor)

    北隆館  2022.04 ISBN: 4832610120

    ASIN

Research Seeds

Research Projects

  • TBD

    Japan Society for the Promotion of Science (JSPS)  JSPS Grant-in-Aids for Scientific Research (C)

    Project Year :

    2024.04
    -
    2027.03
     

  • Insect-Supported Circular Food Production System toward the Global Food Problem and Human Space Exploration

    The Bio-oriented Technology Research Advancement Institution (BRAIN)  Moonshot Agriculture, Forestry and Fisheries Research and Development Program

    Project Year :

    2020.12
    -
    2025.03
     

  • Molecular basis of cognitive aging caused by perturbed endocannabinoid system

    Japan Society for the Promotion of Science (JSPS)  JSPS Grant-in-Aids for Young Scientists

    Project Year :

    2022.04
    -
    2024.03
     

    Kosuke Kataoka

  • Elucidation of the egg diapause mechanism of the Teleogryllus emma based on comparative transcriptome analysis

    Japan Society for the Promotion of Science (JSPS)  JSPS Grant-in-Aids for Scientific Research (C)

    Project Year :

    2021.04
    -
    2024.03
     

  • コオロギ原料の高機能化による代替タンパク質創出

    生物系特定産業技術研究支援センター(BRAIN)  スタートアップ総合支援プログラム(SBIR支援)

    Project Year :

    2023
     
     
     

    株式会社ECOLOGGIE

  • The Regulatory mechanism of cannabinoid CB1 receptor-mediated mitophagy and its involvement in age-dependent memory deficits

    Japan Society for the Promotion of Science (JSPS)  JSPS Grant-in-Aids for Young Scientists

    Project Year :

    2019.04
    -
    2022.03
     

    Kosuke Kataoka

  • Development of Highly Functional Raw Materials Using Insect Crickets from Discarded Materials

    New Energy and Industrial Technology Development Organization (NEDO)  NEDO Entrepreneurs Program (NEP)

    Project Year :

    2021.04
    -
    2021.09
     

  • Genome-wide Analysis on Agricultural Insect Resources

    Joint Research under the Waseda-Fujitsu Laboratories Collaboration Agreement

    Project Year :

    2020.04
    -
    2021.03
     

  • Molecular mechanism of Cereblon-mediated mitophagy in neurons

    Waseda Research Institute for Science and Engineering  Early Bird - Young Scientists' Community

    Project Year :

    2017.06
    -
    2018.03
     

    Kosuke Kataoka

  • Ultra thin membrane monitoring skin health

    Japan Science and Technology Agency  Program for Creating STart-ups from Advanced Research and Technology

    Project Year :

    2016
     
     
     

    Kosuke Kataoka

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Misc

  • Whole genome sequencing and multi-omics analysis of starvation response in Neoseiulus californicus.

    武田直樹, 新井優香, 鈴木伽奈, 片岡孝介, 由良敬, 由良敬, 白藤(梅宮)梨可, GHAZY N.A., 森光太郎, 刑部正博, 鈴木丈詞

    日本ダニ学会誌   32 ( 1 )  2023

    J-GLOBAL

  • Exploration of the taurine biosynthetic pathway in the cricket superfamily

    鈴木愛菜, 伊倉貞吉, 三野流斗, 片岡孝介, 朝日透, 鈴木丈詞, 森光康次郎, 由良敬, 由良敬, 由良敬, 由良敬

    日本分子生物学会年会プログラム・要旨集(Web)   46th  2023

    J-GLOBAL

Other

  • 早稲田大学「学術的文章の作成」文章指導員

    2015.04
    -
    2016.07
 

Syllabus

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Teaching Experience

  • Molecular Biology 51

    Waseda University  

    2023.10
    -
    Now
     

  • Introduction to Biomaterial Analysis/Advanced Solid State Bioscience

    Waseda University  

    2023.04
    -
    Now
     

  • Molecular Cell Biology B

    Waseda University  

    2023.04
    -
    Now
     

  • Design Thinking 02

    Waseda University  

    2022.10
    -
    Now
     

  • Innovation and Technology Practice alpha

    Waseda University  

    2022.10
    -
    Now
     

  • Design Thinking 01

    Waseda University  

    2022.04
    -
    Now
     

  • Innovation and Technology Start-Up alpha

    Waseda University  

    2022.04
    -
    Now
     

  • Special Lecture in BiologyⅩⅢ

    Ochanomizu University  

    2024.04
    -
    2024.09
     

  • Seminar on Problem Solving

    Waseda University  

    2022.10
    -
    2023.03
     

  • Research Design and Analysis

    Waseda University  

    2022.10
    -
    2023.03
     

  • Advanced Ⅰ・Ⅱ・Ⅲ

    Tokyo University of Agriculture and Technology  

    2021.10
    -
    2022.03
     

  • 分子バイオテクノロジー特論

    長浜バイオ大学  

    2021.04
    -
    2021.09
     

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Sub-affiliation

  • Faculty of Science and Engineering   School of Advanced Science and Engineering

  • Affiliated organization   Global Education Center

  • Faculty of International Research and Education   School of International Liberal Studies

Internal Special Research Projects

  • カンナビノイドCB1受容体が制御するミトコンドリアダイナミクス・オートファジーの分子基盤

    2020  

     View Summary

    カンナビノイド受容体CB1をノックアウトしたCB1-KOマウスは海馬において成熟期特異的にマイトファジーが減少していることが分かった。また、分裂と融合によって制御されるミトコンドリアの2D&3D形態を調べたところ、成熟期のCB1-KOではミトコンドリアの伸長性・相互接続性が顕著に増加していることが示唆された。以上より、CB1受容体は加齢依存的にミトコンドリア品質を管理していることが示唆された。本成果は2020年7月にBrain Research bulletin誌に報告した。さらに、見出された現象の分子メカニズムを調べるため、ヒト神経芽細胞腫細胞SH-SY5Yを用いたin vitro実験系を立ち上げた。その結果、CB1受容体の抑制はミトコンドリアの形態を制御することが明らかとなった。

  • タイワンエンマコオロギの全ゲノム塩基配列解読と水圏生産に向けた応用

    2019  

     View Summary

    今後急増する人口を支える代替動物性タンパク質源として、国連は昆虫の利用を強く推奨している。コオロギは、(1)雑食性であり、(2)逆遺伝学的手法が確立され、(3)多くの動物の嗜好に合い、その成長に重要な栄養素を豊富に含むため、特に有望な生物資源として注目を集めている。本研究では、東南アジアで食用として飼育されているタイワンエンマコオロギ(Teleogryllus occipitalis)に着目し、そのゲノム全塩基配列解読を試みた。次世代シーケンサー(イルミナ社NovaSeq 6000およびナノポア社MinION)を用いて得た配列データをde novoアセンブル後、スキャフォールディングした。その結果、遺伝子の高い網羅性を有するアセンブリゲノムおよび遺伝子セットが完成した。本成果は、DDBJやfigshareなどのデータレポジトリに公開登録され、国際論文誌に査読中である。

  • カンナビノイド受容体CB1のマイトファジーへの関与

    2019  

     View Summary

    先進国で高齢化が加速度的に進む中で、老化研究の重要性は高まりつつある。本研究では、加齢に伴う記憶能力の低下に焦点を当てて、その機構の解明を目指している。脳内で最も発現しているGタンパク質共役型受容体である1型カンナビノイド受容体(CB1受容体)は、脳の老化を抑制することが知られているが、その機構は不明である。また2016年、CB1受容体が海馬神経細胞のミトコンドリアにも発現していることや、ミトコンドリア局在CB1受容体が記憶に重要な役割を果たすことが示された。そこで本研究では、CB1受容体のミトコンドリア品質管理における役割を解析した。その結果、CB1受容体は、成熟期特異的にマウス海馬神経細胞におけるミトコンドリアのオートファジーおよびダイナミクス(分裂と融合による形態調節)を制御していることが明らかとなった。本研究成果は、Brain Research Bulletin誌に採択された(Kataoka et al., in press)。

  • 内在性カンナビノイド受容体CB1によるミトコンドリア品質管理機構の解明

    2018   Andreas Zimmer, Andras Bilkei-Gorzo

     View Summary

    本研究では、加齢に伴い急速に記憶・学習能力が低下するカンナビノイド受容体CB1ノックアウトマウスを用いて、CB1が関与する記憶・学習能力の加齢変化のメカニズムを調べた。CB1は海馬神経細胞で機能不全ミトコンドリアの分解(マイトファジー)を促進することで細胞内のエネルギー恒常性を保っていると仮説を立てているため、マイトファジーに関連するタンパク質を生化学・細胞生物学的手法を用いて解析した。その結果、CB1は海馬の神経細胞でマイトファジーの引き金となるリン酸化ユビキチン量の増加に寄与していることが示唆されたが、詳しい分子メカニズムの解明までには至らなかった。本成果は、国内外の学会・シンポジウムにて発表した。