Updated on 2022/05/17

写真a

 
MIYAKE, Takeo
 
Affiliation
Faculty of Science and Engineering, Graduate School of Information, Production, and Systems
Job title
Professor

Concurrent Post

  • Faculty of Science and Engineering   School of Fundamental Science and Engineering

Research Institute

  • 2020
    -
    2022

    理工学術院総合研究所   兼任研究員

Education

  • 2006.04
    -
    2008.03

    Waseda University   Graduate School   Nanoscience & Nanoengineering  

  • 2004.04
    -
    2006.03

    Waseda University   Graduate School   Nanoscience & Nanoengineering  

  • 2000.04
    -
    2004.03

    Waseda University   Science and Engineering Department   Electrical Engineering  

Degree

  • Waseda University Science and Engneering Department   B.S., Electrical Engineering

  • Waseda University Nanoscience & Nanoengineering   M.S. Nanoscience & Nanoengineering

  • Waseda University Nanoscience & Nanoengineering   PhD, Nanoscience & Nanoengineering

Research Experience

  • 2021.04
    -
    Now

    Waseda University   Graduate School of Information Production and Systems

  • 2016.03
    -
    2021.03

    Waseda University   Graduate School of Information, Production and System   Associate Professor

  • 2014.04
    -
    2016.03

    University of Washington   Material Science and Engineering   Acting Instructor

  • 2008.04
    -
    2016.03

    Waseda University   the Nano Technology Reserch Center   Visiting Resercher

  • 2015.10
    -
    2016.02

    University of California, Santa Cruz   Electrical Engineering   Reserch Associate

  • 2009.04
    -
    2014.03

    Tohoku University   Department of Bioengineering and Robotics   Assistant Professor

  • 2008.04
    -
    2009.03

    Japan Society for the Promotion of Science   Reserch Fellowships for Young Scientists, Postdoc

  • 2006.04
    -
    2008.03

    Japan Society for the Promotion of Science   Reserch Fellowships for Young Scientists, Doctoral course students

▼display all

Professional Memberships

  •  
     
     

    Materials Reserch Society

  •  
     
     

    The Surface Science Society of Japan

  •  
     
     

    The Japan Society of Applied Physics

 

Research Areas

  • Biomedical engineering

Research Interests

  • Bioiontronics

  • 無線給電

  • Biofuel Battery

  • Bioprotonics

  • Bioelectronics

Papers

  • Fiber-crafted biofuel cell bracelet for wearable electronics

    Sijie Yin, Xiaohan Liu, Tatsuya Kaji, Yuta Nishina, Takeo Miyake

    BIOSENSORS & BIOELECTRONICS   179  2021.05

     View Summary

    Wearable devices that generate power using sweat have garnered much attention in the field of skin electronics. These devices require high performance with a small volume and low production rate of sweat by living or-ganisms. Here we demonstrate a high-power biofuel cell bracelet based on the lactate in human sweat. The biofuel cell was developed by using a lactate oxidase/osmium-based mediator/carbon nanotube fiber for lactate oxidation and a bilirubin oxidase/carbon nanotube fiber for oxygen reduction; the fibers were woven into a hydrophilic supportive textile for sweat storage. The storage textile was sandwiched between a hydrophobic textile for sweat absorption from the skin and a hydrophilic textile for water evaporation to improve sweat collection. The performance of the layered cell was 74 ?W at 0.39 V in 20 mM artificial sweat lactate, and its performance was maintained at over 80% for 12 h. Furthermore, we demonstrated a series-connection between anode/cathode fibers by tying them up to wrap the bracelet-type biofuel cell on the wrist. The booster six-cell bracelet generated power at 2.0 V that is sufficient for operating digital wrist watches.

    DOI

  • Hybrid power sources for wearable IoT devices

    Taiki TAKAMATSU, Takeo MIYAKE

    Denki Kagaku   88 ( 4 ) 331 - 336  2020.12

    DOI

  • A needle-type biofuel cell using enzyme/mediator/carbon nanotube composite fibers for wearable electronics

    Sijie Yin, Xiaohan Liu, Yuka Kobayashi, Yuta Nishina, Ryo Nakagawa, Ryoji Yanai, Kazuhiro Kimura, Takeo Miyake

    BIOSENSORS & BIOELECTRONICS   165  2020.10

     View Summary

    To realize direct power generation from biofuels in natural organisms, we demonstrate a needle-type biofuel cell (BFC) using enzyme/mediator/carbon nanotube (CNT) composite fibers with the structure Osmium-based polymer/CNT/glucose oxidase/Os-based polymer/CNT. The composite fibers performed a high current density (10 mA/cm(2)) in 5 mM artificial blood glucose. Owing to their hydrophilicity, they also provided sufficient ionic conductivity between the needle-type anode and the gas-diffusion cathode. When the tip of the anodic needle was inserted into natural specimens of grape, kiwifruit, and apple, the assembled BFC generated powers of 55, 44, and 33 mu W from glucose, respectively. In addition, the power generated from the blood glucose in mouse heart was 16.3 mu W at 0.29 V. The lifetime of the BFC was improved by coating an anti-fouling polymer 2-methacryloyloxyethyl phosphorylcholine (MPC) on the anodic electrode, and sealing the cathodic hydrogel chamber with medical tape to minimize the water evaporation without compromising the oxygen permeability.

    DOI

  • Multifunctional High-Power Sources for Smart Contact Lenses

    Taiki Takamatsu, Yin Sijie, Fang Shujie, Liu Xiaohan, Takeo Miyake

    ADVANCED FUNCTIONAL MATERIALS   30 ( 29 )  2020.07  [Refereed]

     View Summary

    Powering an electrical contact lens is a significant challenge for wearable applications such as augmented reality displays and iontophoretic drug delivery to the eye. Here a hybrid power generation device is developed comprising a wireless power transfer system and a bioabsorbable metal-air primary battery, which provides a multifunctional direct current (DC) and/or alternating current (AC) output. The DC power is generated by Zn loop anode and a bilirubin oxidase (BOD) biocathode in an artificial tear. The Zn-based loop anode is also used as the antenna of a wireless power transfer system that results in high power transfer efficiency of 17.6% at 13.56 MHz. The wireless-powered AC voltage is boosted from 1.5 to 1.5 V + 0.5 V-pp by a DC offset, enabling red light-emitting diode (LED) emission. Furthermore, the hybrid AC and DC offset voltages are boosted to 2.3 V + 0.5 V-pp by a capacitive booster circuit that provides blue LED emission. No hydrogen evolution or pH change is observed in the tear electrolyte. The present hybrid power source can potentially power wearable electronics in body fluids.

    DOI

  • Wearable high-powered biofuel cells using enzyme/carbon nanotube composite fibers on textile cloth

    Sijie Yin, Zewen Jin, Takeo Miyake

    BIOSENSORS & BIOELECTRONICS   141  2019.09  [Refereed]

     View Summary

    Wearable biofuel cells with flexible enzyme/carbon nanotube (CNT) fibers were designed on a cotton textile cloth by integrating two components: bioanode fibers for glucose oxidation and O-2-diffusion biocathode fibers for oxygen reduction. The anode and cathode fibers were prepared through modification with glucose dehydrogenase and bilirubin oxidase, respectively, on multi-walled carbon nanotube-coated carbon fibers. Both biofibers woven on the cloth generated a power density of 48 mu W/cm(2) at 0.24 V from 0.1 mM glucose (human sweat amount), and of 216 mu W/cm(2) at 0.36 V, when glucose was supplied from a hydrogel tank containing 200 mM glucose. Our fiber-based biofuel cell deformed to an S-shape without a significant loss in cell performance. Furthermore, we demonstrated a series-connection involving the tying of biofibers on a cloth with batik-based ionic isolation. The booster four cells generate power at 1.9 V that illuminated an LED on the cloth.

    DOI

  • Nanostraw membrane stamping for direct delivery of molecules into adhesive cells

    Bowen Zhang, Yiming Shi, Daisuke Miyamoto, Koji Nakazawa, Takeo Miyake

    SCIENTIFIC REPORTS   9  2019.05  [Refereed]

     View Summary

    Delivering ions and molecules into living cells has become an important challenge in medical and biological fields. Conventional molecular delivery, however, has several issues such as physical and chemical damage to biological cells. Here, we present a method of directly delivering molecules into adhesive cells with an Au-based nanostraw membrane stamp that can physically inject a target molecule into the cytoplasm through a nanostraw duct. We successfully delivered calcein target molecules into adhesive cells with high efficiency (85%) and viability (90%). Furthermore, we modeled the molecular flow through Au nanostraws and then demonstrated the control of calcein flow by changing the concentration and geometry of Au nanostraws. Our Au membrane stamping provides a new way of accessing the cytoplasm to modulate cellular functions via injected molecules.

    DOI

  • Highly Efficient, Flexible Wireless-Powered Circuit Printed on a Moist, Soft Contact Lens

    Taiki Takamatsu, Yunhan Chen, Toshihiko Yoshimasu, Matsuhiko Nishizawa, Takeo Miyake

    ADVANCED MATERIALS TECHNOLOGIES   4 ( 5 ) 1800671-1 - 1800671-8  2019.05  [Refereed]

     View Summary

    Contact lens with built-in electronics is a next-generation wearable product with potential applications such as biomedical sensing and wearable displays. However, fabricating a wireless-powered circuit on a moist, soft contact lens, via common dry lithography, makes producing smart contact lenses challenging. Here, electrochemically (EC) printing a wireless-powered circuit onto a moist, soft contact lens is demonstrated. EC printing involves adding a conductive polymer at the interface between a metal contact and a hydrogelbased contact lens, resulting in strong adhesion of the circuit to the lens without losing high power transfer efficiency (50%) from an eyeglass transmitter to the printed receiver lens. The energy transfer characteristics during eye movement are modeled using the Neumann equation and Kirchhoff's voltage law for wireless power transfer. The energy transfer efficiency between the eyeglass transmitter and the printed receiver lens is derived, and illumination of a wireless-powered single light-emitting diode display as a function of eye rotation angle is demonstrated. This work opens the door to integrating more complex circuits at soft contact lens interface to produce smart contact lens with increased functionality.

    DOI

  • Accelerated Wound Healing on Skin by Electrical Stimulation with a Bioelectric Plaster

    Hiroyuki Kai, Takeshi Yamauchi, Yudai Ogawa, Ayaka Tsubota, Takahiro Magome, Takeo Miyake, Kenshi Yamasaki, Matsuhiko Nishizawa

    ADVANCED HEALTHCARE MATERIALS   6 ( 22 )  2017.11  [Refereed]

     View Summary

    Wound healing on skin involves cell migration and proliferation in response to endogenous electric current. External electrical stimulation by electrical equipment is used to promote these biological processes for the treatment of chronic wounds and ulcers. Miniaturization of the electrical stimulation device for wound healing on skin will make this technology more widely available. Using flexible enzymatic electrodes and stretchable hydrogel, a stretchable bioelectric plaster is fabricated with a built-in enzymatic biofuel cell (EBFC) that fits to skin and generates ionic current along the surface of the skin by enzymatic electrochemical reactions for more than 12 h. To investigate the efficacy of the fabricated bioelectric plaster, an artificial wound is made on the back skin of a live mouse and the wound healing is observed for 7 d in the presence and absence of the ionic current of the bioelectric plaster. The time course of the wound size as well as the hematoxylin and eosin staining of the skin section reveals that the ionic current of the plaster leads to faster and smoother wound healing. The present work demonstrates a proof of concept for the electrical manipulation of biological functions by EBFCs.

    DOI

  • Electronic control of H+ current in a bioprotonic device with Gramicidin A and Alamethicin

    Zahra Hemmatian, Scott Keene, Erik Josberger, Takeo Miyake, Carina Arboleda, Jessica Soto-Rodriguez, Francois Baneyx, Marco Rolandi

    NATURE COMMUNICATIONS   7  2016.10  [Refereed]

     View Summary

    In biological systems, intercellular communication is mediated by membrane proteins and ion channels that regulate traffic of ions and small molecules across cell membranes. A bioelectronic device with ion channels that control ionic flow across a supported lipid bilayer (SLB) should therefore be ideal for interfacing with biological systems. Here, we demonstrate a biotic-abiotic bioprotonic device with Pd contacts that regulates proton (H+) flow across an SLB incorporating the ion channels Gramicidin A (gA) and Alamethicin (ALM). We model the device characteristics using the Goldman-Hodgkin-Katz (GHK) solution to the Nernst-Planck equation for transport across the membrane. We derive the permeability for an SLB integrating gA and ALM and demonstrate pH control as a function of applied voltage and membrane permeability. This work opens the door to integrating more complex H+ channels at the Pd contact interface to produce responsive biotic-abiotic devices with increased functionality.

    DOI

  • A Disposable paper breathalyzer with an alcohol sensing organic electrochemical transistor

    Eloise Bihar, Yingxin Deng, Takeo Miyake, Mohamed Saadaoui, George G. Malliaras, Marco Rolandi

    SCIENTIFIC REPORTS   6  2016.06  [Refereed]

     View Summary

    Breathalyzers estimate Blood Alcohol Content (BAC) from the concentration of ethanol in the breath. Breathalyzers are easy to use but are limited either by their high price and by environmental concerns, or by a short lifetime and the need for continuous recalibration. Here, we demonstrate a proof-of-concept disposable breathalyzer using an organic electrochemical transistor (OECT) modified with alcohol dehydrogenase (ADH) as the sensor. The OECT is made with the conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), and is printed on paper. ADH and its cofactor nicotinamide adenine dinucleotide (NAD(+)) are immobilized onto the OECT with an electrolyte gel. When the OECT-breathalyzer is exposed to ethanol vapor, the enzymatic reaction of ADH and ethanol transforms NAD(+) into NADH, which causes a decrease in the OECT source drain current. In this fashion, the OECT-breathalyzer easily detects ethanol in the breath equivalent to BAC from 0.01% to 0.2%. The use of a printed OECT may contribute to the development of breathalyzers that are disposable, ecofriendly, and integrated with wearable devices for real-time BAC monitoring.

    DOI

  • Proton mediated control of biochemical reactions with bioelectronic pH modulation

    Yingxin Deng, Takeo Miyake, Scott Keene, Erik E. Josberger, Marco Rolandi

    SCIENTIFIC REPORTS   6  2016.04  [Refereed]

     View Summary

    In Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an "enzymatic flip-flop" circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. The transducer also controls bioluminescence from firefly luciferase by affecting solution pH.

    DOI

  • Grotthuss mechanisms: from proton transport in proton wires to bioprotonic devices

    Takeo Miyake, Marco Rolandi

    JOURNAL OF PHYSICS-CONDENSED MATTER   28 ( 2 )  2016.01  [Refereed]

     View Summary

    In 1804, Theodore von Grotthuss proposed a mechanism for proton (H+) transport between water molecules that involves the exchange of a covalent bond between H and O with a hydrogen bond. This mechanism also supports the transport of OH- as a proton hole and is essential in explaining proton transport in intramembrane proton channels. Inspired by the Grotthuss mechanism and its similarity to electron and hole transport in semiconductors, we have developed semiconductor type devices that are able to control and monitor a current of H+ as well as OH- in hydrated biopolymers. In this topical review, we revisit these devices that include protonic diodes, complementary, transistors, memories and transducers as well as a phenomenological description of their behavior that is analogous to electronic semiconductor devices.

    DOI

  • Squid beak inspired water processable chitosan composites with tunable mechanical properties

    Xiaolin Zhang, Pegah Hassanzadeh, Takeo Miyake, Jungho Jin, Marco Rolandi

    JOURNAL OF MATERIALS CHEMISTRY B   4 ( 13 ) 2273 - 2279  2016  [Refereed]

     View Summary

    Natural biological composites often couple light weight with tunable and spatially controlled mechanical properties including stiffness, toughness, and hardness. Examples include the toughness of seashells, the hardness of the chiton tooth, and the stiffness gradient of the squid beak. While seashells and the chiton tooth have a mineralized inorganic component, the squid beak is entirely organic. The squid beak is known as one of the hardest fully organic materials. The hydrated squid beak has a large stiffness gradient from soft, at the interface with the squid mouth, to hard at the tip. This gradient occurs from the spatially controlled cross-linking of chitin nanofibers with a protein matrix aided by catecholamines. Here, we introduce a water processable deacetylated chitin composite with tunable mechanical properties from spatially controlled cross-linking assisted by catecholamines. Given the natural abundance of chitin and the ease of water processing, this composite can find applications for bridging mechanically mismatched materials.

    DOI

  • Stretchable biofuel cell with enzyme-modified conductive textiles

    Yudai Ogawa, Yuki Takai, Yuto Kato, Hiroyuki Kai, Takeo Miyake, Matsuhiko Nishizawa

    BIOSENSORS & BIOELECTRONICS   74   947 - 952  2015.12  [Refereed]

     View Summary

    A sheet-type, stretchable biofuel cell was developed by laminating three components: a bioanode textile for fructose oxidation, a hydrogel sheet containing fructose as fuel, and a gas-diffusion biocathode textile for oxygen reduction. The anode and cathode textiles were prepared by modifying carbon nanotube (CNT)-decorated stretchable textiles with fructose dehydrogenase (FDH) and bilirubin oxidase (BOD), respectively. Enzymatic reaction currents of anode and cathode textiles were stable for 30 cycles of 50% stretching, with initial loss of 20-30% in the first few cycles due to the partial breaking of the CNT network at the junction of textile fibers. The assembled laminate biofuel cell showed power of similar to 0.2 mW/cm(2) with 1.2 k Omega load, which was stable even at stretched, twisted, and wrapped forms. (C) 2015 Elsevier B.V. All rights reserved.

    DOI

  • Organic Transdermal Iontophoresis Patch with Built-in Biofuel Cell

    Yudai Ogawa, Koichiro Kato, Takeo Miyake, Kuniaki Nagamine, Takuya Ofuji, Syuhei Yoshino, Matsuhiko Nishizawa

    ADVANCED HEALTHCARE MATERIALS   4 ( 4 ) 506 - 510  2015.03  [Refereed]

    DOI

  • An enzyme logic bioprotonic transducer

    Takeo Miyake, Erik E. Josberger, Scott Keene, Yingxin Deng, Marco Rolandi

    APL MATERIALS   3 ( 1 )  2015.01  [Refereed]

     View Summary

    Translating ionic currents into measureable electronic signals is essential for the integration of bioelectronic devices with biological systems. We demonstrate the use of a Pd/PdHx electrode as a bioprotonic transducer that connects H+ currents in solution into an electronic signal. This transducer exploits the reversible formation of PdHx in solution according to PdH <-> Pd + H+ + e(-), and the dependence of this formation on solution pH and applied potential. We integrate the protonic transducer with glucose dehydrogenase as an enzymatic AND gate for glucose and NAD(+). PdHx formation and associated electronic current monitors the output drop in pH, thus transducing a biological function into a measurable electronic output. C 2014 Author(s).

    DOI

  • Taking electrons out of bioelectronics: bioprotonic memories, transistors, and enzyme logic

    Zahra Hemmatian, Takeo Miyake, Yingxin Deng, Erik E. Josberger, Scott Keene, Rylan Kautz, Chao Zhong, Jungho Jin, Marco Rolandi

    JOURNAL OF MATERIALS CHEMISTRY C   3 ( 25 ) 6407 - 6412  2015  [Refereed]

     View Summary

    The ability of bioelectronic devices to conduct protons and other ions opens up opportunities to interface with biology. In this research highlight, we report on our recent efforts in bioprotonic devices. These devices monitor and modulate a current of protons with an applied voltage. Voltage-controlled proton flow mimics semiconductor devices with complementary transistors or biological behaviors such as synaptic-like memories and enzyme logic.

    DOI

  • Miniature Enzymatic Fuel Cells

    Takeo Miyake, Matsuhiko Nishizawa

    Enzymatic Fuel Cells: From Fundamentals to Applications     361 - 373  2014.05  [Refereed]

     View Summary

    Enzymatic fuel cells (EFCs) are power devices in which enzymes are used as electrocatalysts to convert biochemical energy directly into electricity, in contrast to metallic catalysts commonly used in fuel cells. This chapter describes the three types of MEFCs fabricated using a series of microelectromechanical system (MEMS)-related techniques. An insertion miniature enzymatic fuel cells (MEFCs) is a type of cell that generates electricity from sugars in living organisms. A microfluidic MEFC consists of microchannels for continuous fuel supply, in which the power generation and performance depend on several fluidic parameters including the flow velocity, electrode configuration, and channel dimensions. The series and parallel stacking of microfluidic MEFCs increase the level of output voltage and net lifetime, respectively. Finally, a sheet-shaped MEFC is described that could be combined with wearable electronics of the future. Engineering advances focused on miniaturization described to promote early practical applications and commercialization of EFCs.

    DOI

  • Surfactant-assisted direct electron transfer between multi-copper oxidases and carbon nanotube-based porous electrodes

    Yudai Ogawa, Syuhei Yoshino, Takeo Miyake, Matsuhiko Nishizawa

    PHYSICAL CHEMISTRY CHEMICAL PHYSICS   16 ( 26 ) 13059 - 13062  2014  [Refereed]

     View Summary

    The effects of pre-treatment with surfactants on the electrocatalytic reaction of multi-copper oxidases were quantitatively evaluated using a well-structured carbon nanotube forest electrode. It was found that both the charge polarity of the head group and the aromatics in the tail part of the surfactants affect the efficiency of enzymatic electrocatalysis.

    DOI

  • Flexible, layered biofuel cells

    Takeo Miyake, Keigo Haneda, Syuhei Yoshino, Matsuhiko Nishizawa

    BIOSENSORS & BIOELECTRONICS   40 ( 1 ) 45 - 49  2013.02  [Refereed]

     View Summary

    Similar to conventional electrolyte batteries, biofuel cells often need to be stacked in order to boost their single cell voltage (<1 V) up to a practical level. Here, we report a laminated stack of biofuel cells that is composed of bioanode fabrics for fructose oxidation, hydrogel sheets containing electrolyte and fuel (fructose), and O-2-diffusion biocathode fabrics. The anode and cathode fabrics were prepared by modifying fructose dehydrogenase and bilirubin oxidase, respectively, on carbon nanotubes-decorated carbon fiber fabrics. The total thickness of the single set of anode/gel/cathode sheets is just 1.1 mm. The laminated triple-layer stack produces an open-circuit voltage of 2.09 V. which is a 2.8-fold increase over that of a single set cell (0.74 V). The present layered cell (5 mm x 5 mm) produces a maximum power of 0.64 mW at 1.21 V. a level that is sufficient to drive light-emitting diodes. (C) 2012 Elsevier B.V. All rights reserved.

    DOI

  • Self-powered sugar indicator using CNT-enzyme ensemble film

    Y. Ogawa, S. Yoshino, K. Kato, T. Magome, T. Yamada, T. Miyake, K. Hata, M. Nishizawa

    Journal of Physics: Conference Series   476 ( 1 )  2013  [Refereed]

     View Summary

    We report the stepwise modification of Os-complex mediator (polyvinylimidazole-[Os(bipyridine)2Cl] (PVI-[Os(bpy)2Cl]) and glucose oxidase (GOD) within the inner nano-space of a carbon nanotube forest (CNTF) film. Owing to the controlled alignment of enzyme/mediator/ electrode in the ensemble, the prepared film electrode has both a high-efficiency (turnover rate of ca. 650 s-1) and a large net oxidation current (ca. 15 mA cm-2). The previous GOD electrodes developed by monolayer-based and polymer-based approaches have either of the performances (efficiency or net activity). In addition, the present GOD electrode is a flexible film that could be used by winding on needle devices. © Published under licence by IOP Publishing Ltd.

    DOI

  • Molecularly Ordered Bioelectrocatalytic Composite Inside a Film of Aligned Carbon Nanotubes

    Syuhei Yoshino, Takeo Miyake, Takeo Yamada, Kenji Hata, Matsuhiko Nishizawa

    ADVANCED ENERGY MATERIALS   3 ( 1 ) 60 - 64  2013.01  [Refereed]

     View Summary

    Molecularly ordered composites of polyvinylimidazole-[Os(bipyridine)2Cl] (PVI-[Os(bpy)2Cl]) and glucose oxidase (GOD) are assembled inside a film of aligned carbon nanotubes. The structure of the prepared GOD/PVI-[Os(bpy)2Cl]/CNT composite film is entirely uniform and stable; more than 90% bioelectrocatalytic activity could be maintained even after storage for 6 d. Owing to the ideal positional relationship achieved between enzyme, mediator, and electrode, the prepared film shows a high bioelectrocatalytic activity for glucose oxidation (ca. 15 mA cm-2 at 25 degrees C) with an extremely high electron-transfer turnover rate (ca. 650 s-1) comparable to the value for GOD solutions, indicating almost every enzyme molecule entrapped within the ensemble (ca. 3 x 1012 enzymes in a 1 mm x 1 mm film) can work to the fullest extent. This free-standing, flexible composite film can be used by winding on a needle device; as an example, a self-powered sugar monitor is demonstrated.

    DOI

  • Sheet-shaped biofuel cell constructed from enzyme-modified nanoengineered carbon fabric

    Keigo Haneda, Syuhei Yoshino, Takuya Ofuji, Takeo Miyake, Matsuhiko Nishizawa

    ELECTROCHIMICA ACTA   82   175 - 178  2012.11  [Refereed]

     View Summary

    A strip of carbon fabric (CF) electrode modified with multiwalled carbon nanotubes and subsequently fructose dehydrogenase (FDH) showed an oxidation current density of similar to 11 mA cm(-2) in stirred 200 mM fructose solution. Obtaining a sufficient dispersion of the nanotubes during its modification was found to be critical to ensure such a performance of the FDH anode. For use with this anode, a CF strip modified with ketjenblack (KB) and bilirubin oxidase (BOD) served as a gas-diffusion cathode for the reduction of O-2 from air at a current density of similar to 2 mA cm(-2.) The FDH-modified CF strip and the BOD-modified CF strip were stacked with an agarose film that retained an electrolyte solution and fuel (fructose) to construct a totally flexible sheet-shaped biofuel cell. This assembly allowed bending of 44 degrees without affecting the maximum output power density, 550 mu W cm(-2) obtained at 0.4 V. (C) 2012 Published by Elsevier Ltd.

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  • Conducting Polymer Microelectrodes Anchored to Hydrogel Films

    Yuichiro Ido, Daisuke Takahashi, Masato Sasaki, Kuniaki Nagamine, Takeo Miyake, Piotr Jasinski, Matsuhiko Nishizawa

    ACS MACRO LETTERS   1 ( 3 ) 400 - 403  2012.03  [Refereed]

     View Summary

    We report the fabrication of totally organic hydrogel-based microelectrodes of poly(3,4-ethylenedioxythiophene) (PEDOT), which exhibit a lowered sheet resistivity of about 100 Omega/square. The preparation process starts with the electrodeposition of conductive PEDOT (ca. 20 S cm(-1)) on Pt microelectrodes. After laminating hydrogels onto the PEDOT-modified Pt electrode substrates, a second PEDOT (low conductivity) layer was electrodeposited to anchor the first PEDOT film to the hydrogel. Finally, the hydrogel sheet with PEDOT micropatterns was peeled off by taking advantage of the electroactuation property of PEDOT. The process proved to be versatile, allowing the use of most natural and synthetic hydrogels including agarose, collagen, polyacrylamide, and so on.

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  • Enzymatic biofuel cells designed for direct power generation from biofluids in living organisms

    Takeo Miyake, Keigo Haneda, Nobuhiro Nagai, Yohei Yatagawa, Hideyuki Onami, Syuhei Yoshino, Toshiaki Abe, Matsuhiko Nishizawa

    ENERGY & ENVIRONMENTAL SCIENCE   4 ( 12 ) 5008 - 5012  2011.12  [Refereed]

     View Summary

    Enzymatic biofuel cells have attracted much attention for their potential to directly use biochemical energy sources in living organisms such as animals, fruits, etc. However, generally natural organisms have a skin, and the oxygen concentration in the organisms is lower than that of biofuels like sugars. Here, we fabricated a novel miniature assembly that consists of a needle bioanode for accessing biofuels in organisms through their skins and a gas-diffusion biocathode for utilizing the abundant oxygen in air. The performance of the biocathode was fourfold improved by optimizing its hydrophobicity. The assembled device with four needle anodes for fructose oxidation was inserted into a raw grape, producing a maximum power of 26.5 mu W (115 mu W cm(-2)) at 0.34 V. A light-emitting diode (LED) with the cell served as a self-powered indicator of the sugar level in the grape. Power generation from blood sugar was also investigated by inserting a needle anode for glucose oxidation into a blood vessel in a rabbit ear. Prior coating of the tip of the needle anode with an anti-biofouling agent was effective to stabilize the output power.

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  • Self-Regulating Enzyme-Nanotube Ensemble Films and Their Application as Flexible Electrodes for Biofuel Cells

    Takeo Miyake, Syuhei Yoshino, Takeo Yamada, Kenji Hata, Matsuhiko Nishizawa

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   133 ( 13 ) 5129 - 5134  2011.04  [Refereed]

     View Summary

    Nanostructured carbons have been widely used for fabricating enzyme-modified electrodes due to their large specific surface area. However, because they are random aggregates of particular or tubular nanocarbons, the postmodification of enzymes to their intrananospace is generally hard to control. Here, we describe a free-standing film of carbon nanotube forest (CNTF) that can form a hybrid ensemble with enzymes through liquid-induced shrinkage. This provides in situ regulation of its intrananospace (inter-CNT pitch) to the size of enzymes and eventually serves as a highly active electrode. The CNTF ensemble with fructose dehydrogenase (FDH) showed the oxidation current density of 16 mA cm(-2) in stirred 200 mM fructose solution. The power density of a biofuel cell using the FDH-CNTF anode and the Laccase CNTF cathode reached 1.8 mW cm(-2) (at 0.45 V) in the stirred oxygenic fructose solution, more than 80% of which could be maintained after continuous operation for 24 h. Application of the free-standing, flexible character of the enzyme CNTF ensemble electrodes is demonstrated via their use in the patch or wound form.

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  • Conducting Polymer Electrodes Printed on Hydrogel

    Soichiro Sekine, Yuichiro Ido, Takeo Miyake, Kuniaki Nagamine, Matsuhiko Nishizawa

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   132 ( 38 ) 13174 - 13175  2010.09  [Refereed]

     View Summary

    We report herein the micropatterning of poly(3,4-ethylenedioxythiophene) (PEDOT) on a hydrogel, agarose, to provide a fully organic, moist, and flexible electrode. The PEDOT/agarose electrodes were prepared through two electrochemical processes: electropolymerization of PEDOT into the hydrogel and electrochemical-actuation-assisted peeling. We also present a typical application of the PEDOT/agarose electrode to the cultivation of contractile myotubes.

    DOI

  • Electrodes Combined with an Agarose Stamp for Addressable Micropatterning

    Soichiro Sekine, Shinya Nakanishi, Takeo Miyake, Kuniaki Nagamine, Hirokazu Kaji, Matsuhiko Nishizawa

    LANGMUIR   26 ( 13 ) 11526 - 11529  2010.07  [Refereed]

     View Summary

    We have combined a topographically patterned agarose microstamp with an electrode substrate to develop a novel printing device that internally contains an electrochemical system for a controlled supply of reactive ink to the stamp surface. The 10 wt % agarose gel containing 0.1 M PBS + 25 mM K Br showed suitable elasticity for forming stamps and served as the electrolytic medium for the electrochemical oxidation of Br- to generate H BrO. The electrode substrate patched with an agarose stamp having 50-mu m-high bumps was used for the spatially confined detachment of heparin/polyethyleneimine precoated on glass substrates, followed by micropatterned adsorption of fibronectin. Using the microelectrode array, the addressable micropatterning of protein by the controlled delivery of H BrO to each bump was demonstrated.

    DOI

  • Microfluidic biological fuel cells: Automatic series-connection and relay systems

    S. Yoshino, M. Oike, Y. Yatagawa, K. Haneda, T. Miyake, M. Nishizawa

    IFMBE Proceedings   31   1637 - 1639  2010  [Refereed]

     View Summary

    Enzymatic biofuel cells have attracted attention. However, there are drawbacks to be overcome for practical applications: The lower output voltage and shorter life time. In this paper, I will present our recent progress in MEMS techniques-based challenge to these problems. The possible output voltage of a single biofuel cell is generally lower than 1 V. The series connection of biofuel cells requires a system for ionic isolation between each cell. We will report an automatic air-valve to connect biofuel cells in series based on the air-trapping at a lotus leaf-like superhydrophobic structure that is manufactured between fuel cells arrayed in a microfluidic channel. The lifetime of an operating enzymatic fuel cell is limited typically within one week. We have been developing the parallel-connected biological micro fuel cells, each of which is shielded from fuel solutions by using degradable materials such as PLGA. © 2010 International Federation for Medical and Biological Engineering.

    DOI

  • Automatic, sequential power generation for prolonging the net lifetime of a miniature biofuel cell stack

    Takeo Miyake, Masato Oike, Syuhei Yoshino, Yohei Yatagawa, Keigo Haneda, Matsuhiko Nishizawa

    LAB ON A CHIP   10 ( 19 ) 2574 - 2578  2010  [Refereed]

     View Summary

    A sequential power generation system for prolonging the net lifetime of a miniature biofuel cell stack has been developed. The system consists of layered chambers of enzyme fuel cells designed to be exposed sequentially to fuel solution by automatically switched fuel flow. The cell chambers were initially separated by magnetized plastic covers sealed with a degradable glue, poly(lactic-co-glycolic acid) (PLGA). The time that the cover was opened by attraction with an external magnet, thereby activating the following cell, was adjustable from a few hours to a few weeks by controlling the weight ratio of Fe3O4 in the covers and the molecular weight of PLGA. By using sequential power generation in this way, the power output of the system was stable for longer periods, and therefore the net lifetime of the stack has been extended as compared with that of a single biofuel cell.

    DOI

  • Biofuel cell anode: NAD(+)/glucose dehydrogenase-coimmobilized ketjenblack electrode

    T. Miyake, M. Oike, S. Yoshino, Y. Yatagawa, K. Haneda, H. Kaji, M. Nishizawa

    CHEMICAL PHYSICS LETTERS   480 ( 1-3 ) 123 - 126  2009.09

     View Summary

    We have studied the coimmobilization of glucose dehydrogenase (GDH) and its cofactor, oxidized nicotinamide adenine dinucleotide (NAD(+)), on a ketjenblack (KB) electrode as a step toward a biofuel cell anode that works without mediators. A KB electrode was first treated with a sulfuric acid/nitric acid/water mixture to lower the overvoltage for NADH oxidation, and was next chemically modified with NAD(+) and GDH. The improved GDH/NAD(+)/KB electrode is found to oxidize glucose around 0 V vs. Ag/AgCl. A biofuel cell constructed with a bilirubin oxidase-immobilized KB cathode showed a maximum power density of 52 mu/cm(2) at 0.3 V. (C) 2009 Published by Elsevier B.V.

    DOI

  • Real-time imaging of single-molecule fluorescence with a zero-mode waveguide for the analysis of protein-protein interaction

    Takeo Miyake, Takashi Tanii, Hironori Sonobe, Rena Akahori, Naonobu Shimamoto, Taro Ueno, Takashi Funatsu, Iwao Ohdomari

    ANALYTICAL CHEMISTRY   80 ( 15 ) 6018 - 6022  2008.08  [Refereed]

     View Summary

    Real-time imaging of single-molecule fluorescence with a zero-mode waveguide (ZMW) was achieved. With modification of the ZMW geometry, the signal-to-background ratio is twice that obtainable with a conventional ZMW. The improved signal-to-background ratio makes it possible to visualize individual binding-release events between chaperonin GroEL and cochaperonin GroES at a concentration of 5 mu M. Two rate constants representing two-timer kinetics in the release of GroES from GroEL were measured with the ZMW, and the measurements agreed well with those made with a total internal reflection fluorescence microscopy. These results indicate that the novel ZMW makes feasible the direct observation of protein-protein interaction at an intracellular concentration in real time.

    DOI

  • Effect of the C-terminal truncation on the functional cycle of chaperonin GroEL - Implication that the C-terminal region facilitates the transition from the folding-arrested to the folding-competent state

    Mihoko Suzuki, Taro Ueno, Ryo Iizuka, Takahiro Miura, Tamotsu Zako, Rena Akahori, Takeo Miyake, Naonobu Shimamoto, Mutsuko Aoki, Takashi Tanii, Iwao Ohdomari, Takashi Funatsu

    JOURNAL OF BIOLOGICAL CHEMISTRY   283 ( 35 ) 23931 - 23939  2008.08  [Refereed]

     View Summary

    To elucidate the exact role of the C-terminal region of GroEL in its functional cycle, the C-terminal 20-amino acid truncated mutant of GroEL was constructed. The steady-state ATPase rate and duration of GroES binding showed that the functional cycle of the truncated GroEL is extended by similar to 2 s in comparison with that of the wild type, without interfering with the basic functions of GroEL. We have proposed a model for the functional cycle of GroEL, which consists of two rate-limiting steps of similar to 3- and similar to 5-s duration (Ueno, T., Taguchi, H., Tadakuma, H., Yoshida, M., and Funatsu, T. (2004) Mol. Cell 14, 423-434). According to the model, detailed kinetic studies were performed. We found that a 20-residue truncation of the C terminus extends the time until inorganic phosphate is generated and the time for arresting protein folding in the central cavity, i.e. the lifetime of the first rate-limiting step in the functional cycle, to an similar to 5-s duration. These results suggest that the integrity of the C-terminal region facilitates the transition from the first to the second rate-limiting state.

    DOI

  • Protein adsorption on self-assembled monolayers induced by surface water molecule

    Yuzo Kanari, Yusuke Shoji, Hirotaka Ode, Takeo Miyake, Takashi Tanii, Tyuji Hoshin, Iwao Ohdomari

    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS   46 ( 9B ) 6303 - 6308  2007.09  [Refereed]

     View Summary

    Three types of self-assembled monolayers (SAMs) are employed for the investigation of protein adsorption. An octadecylsilane (ODS) SAM, a fluoroalkylsilane (FAS) SAM, and a polyethylene glycol (PEG) SAM are selected as examples. The amount of adsorbed protein is measured by fluorescent microscopy. A molecular dynamics (MD) simulation is carried out for modeling both the molecular structures of the SAMs and the water structure on the SAMs. A hydrophilic PEG SAM prevents protein adsorption, while a large amount of adsorption is observed on a hydrophobic ODS SAM. In spite of the hydrophobicity, an FAS SAM prevents protein adsorption as well as a PEG SAM. MD calculation suggests that the existence of surface-bound water on an ODS SAM induces protein, adsorption. In the case of the FAS SAM, owing to the electrostatic interaction and the flexibility of the precursor, the water molecule is not bound to the surface and protein adsorption is suppressed.

    DOI

  • Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

    Takeo Miyake, Takashi Tanii, Koichi Kato, Tamotsu Zako, Takashi Funatsu, Iwao Ohdomari

    NANOTECHNOLOGY   18 ( 30 )  2007.08  [Refereed]

     View Summary

    Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

    DOI

  • Analysis of electron beam sensitivity of self-assem bled monolayer resist depending on terminal group

    K. Kato, T. Miyake, Y. Beppu, T. Tanii, I. Ohdomari

    Digest of Papers - Microprocesses and Nanotechnology 2007; 20th International Microprocesses and Nanotechnology Conference, MNC     60 - 61  2007  [Refereed]

    DOI

  • Single molecule imaging of Chaperonin functions using zero-mode waveguides

    Taro Ueno, Takashi Tanii, Naonobu Shimamoto, Takeo Miyake, Hironori Sonobe, Iwao Oodomari, Takashi Funatsu

    BIOPHYSICAL JOURNAL     554A - 554A  2007.01  [Refereed]

  • Nanopatterning of hydroxy-terminated self-assembled monolayer taking advantage of terminal group modification

    Takeo Miyake, Takashi Tanii, Koichi Kato, Takumi Hosaka, Yuzo Kanari, Hironori Sonobe, Iwao Ohdomari

    CHEMICAL PHYSICS LETTERS   426 ( 4-6 ) 361 - 364  2006.08  [Refereed]

     View Summary

    The feasibility of using an octenyltrimethoxysilane (OCS) self-assembled monolayer (SAM) as a high-resolution electron beam (EB) resist is investigated. The vinyl groups of the OCS SAM can be modified into hydroxy groups, which are useful for biochip fabrication. The hydroxy-modified OCS SAM exhibits higher sensitivity than a vinyl-terminated one. By using the hydroxy-modified OCS SAM as an EB resist, a miniaturized pattern of 18 nm is achieved. Since the hydroxy-modified OCS SAM is repellent to many proteins, this methodology can be useful for the fabrication of protein-immobilizing templates of a molecular scale. (c) 2006 Elsevier B.V. All rights reserved.

    DOI

  • Nanoscale patterning of protein using electron beam lithography of organosilane self-assembled monolayers

    GJ Zhang, T Tanii, T Zako, T Hosaka, T Miyake, Y Kanari, TW Funatsu, Ohdomari, I

    SMALL   1 ( 8-9 ) 833 - 837  2005.08

    DOI

  • Hybridization of deoxyribonucleic acid and immobilization of green fluorescent protein on nanostructured organosilane templates

    T Tanii, T Hosaka, T Miyake, Y Kanari, GJ Zhang, T Funatsu, Ohdomari, I

    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS   44 ( 7B ) 5851 - 5855  2005.07

     View Summary

    We propose a novel process for preferential immobilization of deoxyribonucleic acid (DNA) and green fluorescent protein (GFP) onto organosilane self-assembled monolayer (SAM) templates. One of the advantages of using the organosilane SAM as the template is that it is electron-beam sensitive and, by utilizing the SAM as an alternative resist film, we can make nanopatterns on a molecular scale because the proximity effect is negligible owing to the film&apos;s thinness. An other advantage is that the organosilane SAM is repellent to both DNA and GFP. Thus, the patterned SAM can be utilized as the passivation film covering the outside of the pattern while DNA and GFP are immobilized within the pattern. We investigate two kinds of organosilane SAMs for the template. One is n-octadecyltrimethoxysilane (ODS) SAM, and the other is 1H,1H,2H,2H-perfluorodecyltriethoxysilane (FDS) SAM. Our results indicate that the ODS SAM is more repellent to DNA than the FDS SAM and is suitable for DNA immobilization, while the FDS SAM template is suitable for GFP immobilization via biotin-avidin coupling because the FDS SAM surface prevents the nonspecific adsorption of streptavidin. Although the nonspecific adsorption of DNA and GFP on a SAM is also recognized, by controlling both the concentration and the incubation time, we can immobilize DNA and GFP preferentially onto nanopatterns of 100 nm diameter.

    DOI

  • Attachment of DNA to microfabricated arrays with self-assembled monolayer

    GJ Zhang, T Tanii, T Miyake, T Funatsu, Ohdomari, I

    THIN SOLID FILMS   464   452 - 455  2004.10

     View Summary

    A novel approach of fabricating sub-10-mum patterns on silicon surfaces by electron beam (EB) lithography for attachment of oligonucleotides was described. The shape of the microfabricated arrays was observed to be regular by optical microscopy. An octadecyltrmiethoxysilane (ODS) monolayer was deposited on the regions outside the patterned areas to minimize the nonspecific binding of biomolecules. Cy 5-labeled target DNA was hybridized to both complementary and noncomplementary oligonucleotides that were covalently anchored to micropatterns. As a result, the micropatterns where specific binding occurred show strong signals, whereas no signals are observed in the case of nonspecific binding. These data indicate that miniature micro- and nano-arrays will find applications in biochips and biosensors. (C) 2004 Elsevier B.V. All rights reserved.

    DOI

  • Preferential immobilization of biomolecules on silicon microstructure array by means of electron beam lithography on organosilane self-assembled monolayer resist

    T Tanii, T Hosaka, T Miyake, GJ Zhang, T Zako, T Funatsu, Ohdomari, I

    APPLIED SURFACE SCIENCE   234 ( 1-4 ) 102 - 106  2004.07

     View Summary

    A novel fabrication process of silicon microstructure array for preferential immobilization of biomolecules is proposed. We perform electron beam lithography on a self-assembled monolayer (SAM), and achieve high-density silicon patterns terminated with both 3-aminopropyltriethoxysilane (APTES) and octadecyltrimethoxysilane (ODS). The amino-terminated surface produces the site-directed covalent immobilization of DNA inside the pattern, while the hydrophobic surface of the ODS-SAM prevents the adsorption. As a result, we have succeeded in immobilizing the DNA within the amino-modified area. By using this methodology, we demonstrate the miniaturization of deoxyribonucleic acid (DNA) chip. After the covalent attachment of the amino-modified oligonucleotides to the microstructures, we hybridize the immobilized DNA with the target DNA labeled with a fluorescent dye. The signals from the DNA chip exhibit the specific binding due to the DNA-DNA interaction. These results show the feasibility of this technique for high-density information storage and biochip miniaturization. (C) 2004 Elsevier B.V. All rights reserved.

    DOI

  • Electron beam lithography on organosilane self-assembled monolayer resist

    T Tanii, T Hosaka, T Miyake, Ohdomari, I

    JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS   43 ( 7A ) 4396 - 4397  2004.07

     View Summary

    We report a result of a feasibility study on the application of an octadecyltrimethoxysilane, self-assembled monolayer to a resist film for electron beam lithography. The self-assembled monolayer deposited on a silicon dioxide surface by chemical vapor deposition is resistant to both sulfuric acid and hydrofluoric acid. By immersing the electron-beam-irradiated surface into both acids, we successfully develop microstructural patterns in the self-assembled monolayer. In particular, we show the effectiveness of immersing the substrate into a sulfuric-acid-based solution for the development of the pattern. The relationship between the required dose and the developing time is estimated by measuring the morphology of the developed patterns by atomic force microscopy. The pattern in the self-assembled monolayer can be transferred into both the underlying silicon dioxide layer and the silicon substrate. These results indicate that the organosilane self-assembled monolayer serves as an alternative resist for electron beam lithography.

    DOI

  • A protonic biotransducer controlling mitochondrial ATP synthesis

    Zhang, Z, Kashiwagi, H, Kimura, S, Kong, S, Ohta, Y, Miyake, T

    Scientific Reports, 8 10423, 2018.  

    DOI

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Books and Other Publications

  • ソフト酵素電極によるオール有機バイオ発電パッチ

    吉田昭太郎, 西澤松彦, 三宅丈雄( Part: Joint author)

    シーエムシー出版、「酵素トランスデューサーと酵素技術展開」pp.1-10,2020  2020

  • Grotthuss Mechanism: From Proton Transport in Ion Channels to Bioprotonic Devices(ch. 8), in Green Materials for Electronics

    Miyake, T, Rolandi, M

    Wiley-VCH, Weinheim, Germany  2017

  • “柔らかい酵素電池”表面科学会誌

    三宅丈雄, 小川雄大, 西澤松彦

    表面科学会  2014

  • ”酵素反応で発電する無害・安全なバイオ電池”「スマート・ヒューマンセンシング ~健康ビッグデータ時代のためのセンサ・情報・エネルギー技術~」

    三宅丈雄, 西澤松彦

    シーエムシー出版  2014

  • Miniature Enzymatic Fuel Cells(ch. 17) in Enzymatic Fuel Cells From Fundamentals to Applications

    Miyake, T, Nishizawa, M

    Wiley-VCH, Weinheim, Germany  2014

  • “酵素を使ったバイオ発電の最新動向” 電気学会論文誌E(センサ・マイクロマシン部門誌)vol.133

    三宅丈雄, 西澤松彦

    電気学会  2013

  • “小型酵素電池の最前線”

    三宅丈雄, 西澤松彦

    化学と工業  2012

  • “電池マテリアルとして期待される「発電酵素」 ~生体や環境にやさしい酵素電池の最前線~” vol.17

    三宅丈雄, 西澤松彦

    マテリアルステージ  2012

  • “環境・生体に優しいバイオ燃料電池”「静電気学会誌」

    三宅丈雄, 吉野修平, 西澤松彦

    静電気学会  2011

  • “医療用マイクロ酵素電池”「バイオ電池の最新動向」

    三宅丈雄, 吉野修平, 西澤松彦

    シーエムシー出版  2011

  • “カーボンナノチューブ電極”「バイオ電池の最新動向」

    吉野修平, 三宅丈雄, 西澤松彦

    シーエムシー出版  2011

  • “バイオ電池の最新開発動向”プレスジャーナル vol.29

    三宅丈雄, 西澤松彦

    プレスジャーナル  2010

  • “バイオ燃料電池”電子情報通信学会「知識ベース」

    三宅丈雄, 西澤松彦

    電子情報通信学会  2010

  • “バイオ燃料電池”「エネルギーハーベスティングの最新動向」

    三宅丈雄, 西澤松彦

    シーエムシー出版  2010

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Misc

  • 細胞穿刺用ナノストロメンブレンの作成と接着細胞への物質導入

    SHI Yiming, ZHANG Bowen, 宮本大輔, 太田裕介, 中澤浩二, 三宅丈雄

    日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM)   2018  2018

    J-GLOBAL

  • 179 Cnducting polymer microelectrodes anchored to hydrogel films and its electrical applications

    SASAKI Masato, Takahashi Daisuke, NAGAMINE Kuniaki, MIYAKE Takeo, KAJI Hirokazu, NISHIZAWA Matsuhiko

      2013 ( 48 ) 160 - 160  2013.03

    CiNii

  • 189 Flexible, miniature biofuel cell

    OFUJI Takuya, YOSHINO Shuhei, KATO Koichiro, MIYAKE Takeo, KAJI Hirokazu, NISHIZAWA Matsuhiko

      2013 ( 48 ) 180 - 180  2013.03

    CiNii

  • Device technologies for electrical power generation from biochemical resources

    Takeo Miyake, Matsuhiko Nishizawa

    IEEJ Transactions on Sensors and Micromachines   133 ( 9 ) 242 - 247  2013

    Book review, literature introduction, etc.  

     View Summary

    Electrical power generation from carbohydrates produced by living systems is one of attractive green energy technologies, and has motivated for decades the development of enzymatic biofuel cells (eBFC) that can directly generate electricity without purification of the biofluids. In this review, we explain resent progress of enzymatic power generation in-vitro and in-vivo, and then describe the perspective of eBFC system with particular emphasis on the performance and stability requirements toward medical applications.

    DOI

  • Biocompatible Biofuel Cell

    MIYAKE Takeo, YOSHINO Syuhei, NISHIZAWA Matsuhiko

    Journal of the Institute of Electrostatics Japan   35 ( 5 ) 214 - 219  2011.09

    CiNii

Awards

  • 文部科学大臣表彰若手科学者賞

    2020   文部科学省  

    Winner: 三宅丈雄

  • Poster award

    2018   Nature Conference on Flexible Electronics -Visions of a Flexible Future-   Wireless-Powered, Biosensing Wearable Smart Soft Contact Lens

    Winner: Takeo Miyake

  • 19th Incentive Prize(Aoba Foundation for the Promotion of Engineering)

    2013.11   Aoba Foundation for the Promotion of Engineering   Self-Powered Sugar Indicator Using enzyme-CNT Ensemble Film

  • Best Presentation Award(The 73rd Autumn Meeting of the Japan Society of Applied Physics)

    2012.09   The Japan Society of Applied Physics   Direct Power Generation from A Biofuel in Living-Organisms

  • Poster Presentation Award(The 10th Annual Meeting of Society of Nano Science and Technology)

    2012.06   The Society of Nano Science and Technology   Enzyme-based biofuel cell and its alpplication to self-powered monitoring of sugar levels in fruits

  • International Symposium on Surface Science(ISSS-6)

    2011.12   The Surface Science Society of Japan   Engineering Enzyme-Nanotube Ensemble Films for Electrical Power Generation from Biofuels in Living-Organisms

  • Best Presentation Award(The 31st Annual Meeting of Surface Science)

    2011.12   The Surface Science Society of Japan   Totally-Organic Microelectrodes for Electrical Stimulation to Muscle cells

  • Poster Presentation Award(The 22nd of Chemistry and Micro-Nano system)

    2010.11   Society for Chemistry and Micro-Nano System   Enzyme Entrapment into Carbon Nanotube Forest and Its Application to Biofuel Cell

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Research Projects

  • 電気化学的pH制御によるミトコンドリア中ATP合成の制御

    Project Year :

    2017.06
    -
    2019.03
     

  • Enzyme-powered patch for wound healing

    Project Year :

    2016.08
    -
    2018.03
     

  • “体液から直接発電するウェアラブル酵素発電デバイスの開発”

    公益財団法人東燃ゼネラル石油研究奨励・奨学財団 

    Project Year :

    2018
    -
     
     

  • “酵素反応駆動による創傷治癒促進デバイスの創出”

    公益財団法人内藤記念科学振興財団 

    Project Year :

    2018
    -
     
     

  • ”電気化学的pH制御によるミトコンドリア中ATP合成の制御”

    Project Year :

    2017
    -
    2018
     

  • “身近な糖を燃料とするバイオ発電デバイスの開発”

    東京医科歯科大学生体材料工学研究所 

    Project Year :

    2016
    -
    2018
     

  • 酵素反応で自立駆動する創傷治癒促進デバイスの創出

    Project Year :

    2014.04
    -
    2017.03
     

  • “生体と調和する無線給電型バイオセンシング素子の開発”

    公益財団法人立石科学技術振興財団 

    Project Year :

    2017
    -
     
     

  • “マイクロ/ナノ空間におけるプロトン濃度の電気化学式精密制御および生体応用”

    公益財団法人精密測定技術振興財団 

    Project Year :

    2017
    -
     
     

  • “酵素発電による創傷治癒促進デバイスの創出”

    Project Year :

    2016
    -
    2017
     

  • “体液から直接発電するユビキタス酵素電池の開発”

    公益財団法人中部電気利用基礎研究振興財団 

    Project Year :

    2016
    -
     
     

  • “生体素子と電子素子をイオンでつなぐ革新的バイオトランスデューサの創出”

    公益財団法人日揮・実吉奨学会研究助成金 

    Project Year :

    2016
    -
     
     

  • “イオン・電子伝導制御に向けたバイオニックFETの創出”

    公益財団法人村田学術振興財団 

    Project Year :

    2016
    -
     
     

  • “ソフトコンタクトレンズ上に印刷される無線バイオセンシング素子の開発”

    公益財団法人大川情報通信基金 

    Project Year :

    2016
    -
     
     

  • “酵素反応で自立駆動する創傷治癒促進デバイスの創出”

    Project Year :

    2014
    -
    2016
     

  • Bionic Transdermal Patch

    Project Year :

    2012.04
    -
    2014.03
     

     View Summary

    We report here a transdermal biofuel cell patch that is composed of bioanode fabrics for fructose oxidation, hydrogel tanks containing fructose fuel and chemical compounds (Rhodamine B and ascorbyl glucoside (AG)), O2-diffusion biocathode fabrics, and an ion-isolating hydrophobic flame. When the patch is mounted on the mammalian skin, the patch generate the ionic current of ca.140 microampere cm-2 between the anode and the cathode across the skin. This ion current could be controlled by setting up the external resistance. The applied current induced a water flow of ca. 7.7 microliter cm-2 for 1 h at small current of 5 microampere cm-2 toward the cathode, and then assisted a chemical administration into the skin (ex. AG delivery of 20 microgram cm-2 within the epidermis and 2.5 microgram cm-2 within the dermis) at the anodic side

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Presentations

  • “酵素/カーボンナノ電極を用いたバイオ発電デバイスと生体応用”

    三宅 丈雄

    第78回秋季応用物理学会「フレキシブル環境発電デバイスの新展開」  (福岡)  応用物理学会

    Presentation date: 2017.09

  • “生体に調和するバイオ発電デバイスの開発と今後の展開”

    三宅 丈雄

    「ウェアラブルデバイス・バイオセンサ応用を目指したバイオ電池の最前線」  (東京)  シーエムシー出版

    Presentation date: 2017.06

  • “バイオイオントロ二クスへの挑戦”

    三宅 丈雄

    第97回春季年会「未来のヘルスケアを支える革新技術;未来医療を支える生体適合性材料」  (東京)  日本化学会

    Presentation date: 2017.03

  • “Organic Biotransducer for Interactive Communication with Biology”

    Miyake, T

    Bioelectronics and Biosensors Congress 2016 

    Presentation date: 2016.11

  • “生体素材と電子素子をイオンでつなぐ革新的バイオイオントロ二クスへの挑戦”

    三宅 丈雄

    「生体エレクトロニクス研究の最前線」  (東京)  有機エレクトロニクス材料研究会

    Presentation date: 2016.09

  • “Bioprotonic devices: bioprotonic transistors, memory and enzyme logic”

    Miyake, T

    14th International Conference on Optical and Electronic Sensors 

    Presentation date: 2016.06

  • “生体と調和するバイオイオントロ二クスへの挑戦”

    三宅 丈雄

    (愛知県名古屋市)  マルチモーダルバイオイメージセンサ研究会

    Presentation date: 2016.05

  • “Biopolymer-based protonics: protonic diode, transistor, synaptic memory and enzymatic logic”

    Miyake, T, Rolandi, M

    Collaborative Conference on 3D and Materials Reserch 

    Presentation date: 2015.06

  • “プロトン濃度勾配で自立駆動する生体模倣エネルギー変換デバイスへの挑戦”

    三宅 丈雄

    第11回ウェットデバイス研究会  (宮城県東北大学) 

    Presentation date: 2015.06

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Specific Research

  • プロトン伝導制御技術の構築とバイオデバイス応用

    2018  

     View Summary

    本研究課題では,これまでのPdと比してプロトンに対する選択性が高く,かつ,酸化還元電位を下げることのできる導電性高分子(スルホン化ポリアニリン)を開発し,本Protode電極を用いることで溶液中のpHを精密に制御(10mM Tris-HCl緩衝液中でpH変化を0.1単位で制御)することに成功した.さらに,本電極と細胞から単離したミトコンドリアを組み合わせることで,電気化学的にミトコンドリア内ATPの合成を制御することに,世界で初めて実現した.

  • 生体と調和する無線給電型バイオセンシングレンズの開発

    2017  

     View Summary

    本研究課題では,水分に影響しない13.56MHzにおける無線給電のためのループアンテナの設計とその電力利得評価に加え,本無線システムとLEDを搭載した電子ソフトコンタクトレンズを実現させた.通常,無線給電はAC電源となるため,直流を必要とするLED用電源には向かない.そこで,AC/DC変換のための整流回路を加えることで,コンタクトレンズ上で負荷なくLEDを点灯させることに成功した.また,眼球の動き(回転)に応じて変化するLED輝度の解析からアイモーションを検出できるシステムも実現した.

  • 電気化学的pH制御による生きたミトコンドリア中ATP合成の制御

    2016  

     View Summary

    本研究では,溶液のプロトン(H+)と親和性の高いprotode電極およびカーボンナノチューブによるスーパーキャパシタ電極を組み合わせることで溶液中のpHを制御できる電気化学的トランスデューサの開発を行い,細胞膜イオンチャネルのイオン伝導性を評価した.

 

Syllabus

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Committee Memberships

  • 2021.11
    -
     

    The 9th International Symposium on Surface Science  Biomaterial interfaces, session chair

  • 2021.11
    -
     

    The 9th International Symposium on Surface Science  Biomaterial interfaces, session chair

  • 2020.12
    -
     

    第30回日本MRS年次大会  シンポジウムオーガナイザー

  • 2020.11
    -
     

    32nd International Microprocesses and Nanotechnology Conference BioMEMS, Lab on a Chip, and Nanobiotechnology  Members

  • 2020.11
    -
     

    32nd International Microprocesses and Nanotechnology Conference BioMEMS, Lab on a Chip, and Nanobiotechnology  Members

  • 2019.12
    -
     

    第28回日本MRS年次大会、シンポジウム「スマート社会・スマートライフのためのバイオセンサ・バイオ燃料電池  共催者

  • 2019.10
    -
     

    32nd International Microprocesses and Nanotechnology Conference BioMEMS, Lab on a Chip, and Nanobiotechnology  Vice chair

  • 2019.10
    -
     

    32nd International Microprocesses and Nanotechnology Conference BioMEMS, Lab on a Chip, and Nanobiotechnology  Vice chair

  • 2019.09
    -
     

    第80回応用物理学会秋季学術講演会「機能材料・萌芽的デバイスセッション」「ナノバイオテクノロジーセッション」  座長、審査員

  • 2018.12
    -
     

    第28回日本MRS年次大会、シンポジウム「スマート社会・スマートライフのためのバイオセンサ・バイオ燃料電池」  共催者

  • 2018.06
    -
     

    ロボティクス・メカトロニクス講演会2018  プログラム委員

  • 2018.03
    -
     

    第65回応用物理学会春季学術講演会「機能材料・萌芽的デバイスセッション」「ナノバイオテクノロジーセッション」  座長および審査

  • 2017
    -
     

    応用物理学会有機分子・バイオエレクトロニクス分科会  幹事

  • 2013.09
    -
     

    第74回応用物理学会秋季学術講演会、「ナノバイオテクノロジーセッション」  座長

  • 2013.05
    -
     

    第27回化学とマイクロ・ナノシステム研究会  実行委員

  • 2013.03
    -
     

    日本機械系学会東北支部第48期総会・講演会、「学生賞Ⅰセッション」  座長および審査委員

  • 2013.03
    -
     

    電気化学会第80回大会  実行委員

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