Updated on 2022/01/24


Affiliated organization, Research Innovation Center
Job title
Associate Professor(without tenure)

Concurrent Post

  • Faculty of Sport Sciences   School of Sport and Sciences



    Shinshu University   Graduate School of Medicine   Major in Physiology  


    Waseda University   Graduate School of Human Sciences   Major in Life Sciences  


    Waseda University   School of Human Sciences   Department of Sport Sciences  




  • Shinshu University   Doctor of Philosophy in Medical Science

Research Experience

  • 2016.04

    Waseda University   Center for Research Strategy

  • 2014.02

    Waseda University   Center for Research Strategy

  • 2008.04


  • 2009.10

    Waseda University   Center for Research Strategy

  • 2007.09

    Waseda University   Advanced Research Center for Human Sciences

  • 2004.09

    Waseda University   School of Human Sciences

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Research Areas

  • Physiology

  • Sports sciences

Research Interests

  • research strategy, research organization management, coaching, softball, sport medical sciences


  • Impact of Intensive High-Fat Ingestion in the Early Stage of Recovery from Exercise Training on Substrate Metabolism during Exercise in Humans

    Takashi Ichinose, Natsuko Arai, Tomoaki Nagasaka, Masaya Asano, Kenji Hashimoto


     View Summary

    Not only increasing body carbohydrate (CHO) stores before exercise but also suppressing CHO oxidation during exercise is important for improving endurance performance. We tested the hypothesis that intensive high-fat ingestion in the early stage of recovery from exercise training (ET) for 2 d would suppress CHO oxidation during exercise by increasing whole body lipolysis and/or fat oxidation. In a randomized crossover design, on days 1 and 2, six male subjects performed cycle ET at 50% peak oxygen consumption (VO2 peak) for 60-90 min, and consumed a control diet (CON: 1,224 kcal, 55% carbohydrate, 30% fat) or the same diet supplemented with high fat (HF: 1.974 kcal, 34% carbohydrate, 56% fat) 1 h after ET. with the diet other than post-ET similar in both trials. On day 3, subjects performed cycle exercise at 65% VO2 peak until exhaustion. Exercise time to exhaustion was longer in the HE trial than in the CON trial (CON: 48.9 +/- 6.7 vs. HF: 55.8 +/- 7.7 min, p<0.05). In the HE trial, total fat oxidation until exhaustion was higher, accompanied by higher post-exercise plasma glycerol concentration, than in the CON trial (CON: 213 54 vs. HF: 286 +/- 63 kcal, p<0.05), whereas total carbohydrate oxidation until exhaustion was not different between trials. These results suggest that intensive high-fat ingestion in the early stage of recovery from ET for a few days until the day before exercise was an effective means of eliciting a CHO-sparing effect during exercise by enhancing fat metabolism.


  • Effect of endurance training supplemented with green tea extract on substrate metabolism during exercise in humans

    T. Ichinose, S. Nomura, Y. Someya, S. Akimoto, K. Tachiyashiki, K. Imaizumi


     View Summary

    Endurance training and ingestion of green tea extract (GTE), composed mainly of tea catechins (TC), are well known to enhance fat metabolism. However, their synergistic effects remain to be fully elucidated. We tested the hypothesis that endurance training supplemented with GTE would further accelerate whole-body fat utilization during exercise, compared with training alone, in humans. Twelve healthy male subjects [peak oxygen consumption ((V) over dotO(2peak)), 50.7 +/- 1.3 (SEM) mL/kg/min] were divided into two groups: GTE and placebo (PLA) groups. Subjects in both groups performed a cycle ergometer exercise at 60% of (V) over dotO(2peak) for 60 min/day, 3 days/week, and daily ingested 572.8 or 0 mg TC in GTE and PLA groups for 10 weeks, respectively. Before and after training, respiratory gas exchange was measured during 90-min exercise at pre-training similar to 55% of (V) over dotO(2peak). After training, the average respiratory exchange ratio during exercise remained unchanged in the PLA group (post-training: 0.834 +/- 0.008 vs pre-training: 0.841 +/- 0.004), whereas it was lower in the GTE group (post-training: 0.816 +/- 0.006 vs pre-training: 0.844 +/- 0.005, P<0.05). These results suggest that habitual GTE ingestion, in combination with moderate-intense exercise, was beneficial to increase the proportion of whole-body fat utilization during exercise.


  • Impact of protein and carbohydrate supplementation on plasma volume expansion and thermoregulatory adaptation by aerobic training in older men

    Kazunobu Okazaki, Takashi Ichinose, Hiroyuki Mitono, Mian Chen, Shizue Masuki, Hiroshi Endoh, Hideki Hayase, Tatsuya Doi, Hiroshi Nose

    JOURNAL OF APPLIED PHYSIOLOGY   107 ( 3 ) 725 - 733  2009.09

     View Summary

    Okazaki K, Ichinose T, Mitono H, Chen M, Masuki S, Endoh H, Hayase H, Doi T, Nose H. Impact of protein and carbohydrate supplementation on plasma volume expansion and thermoregulatory adaptation by aerobic training in older men. J Appl Physiol 107: 725-733, 2009. First published July 16, 2009; doi: 10.1152/japplphysiol.91265.2008.-We examined whether protein-carbohydrate (CHO) supplementation immediately after exercise each day during aerobic training facilitated plasma volume (PV) expansion and thermoregulatory and cardiovascular adaptations in older men. Fourteen moderately active older men [68 +/- 5 (SD) yr] were divided into two groups so as to have no significant differences in anthropometric measures, PV, and peak oxygen consumption rate ((V) over dotO(2peak)). Each group was provided with a mixture of protein and CHO (3.2 kcal, 0.18 g protein/kg body wt, Pro-CHO, n = 7) or a non-protein and low-calorie placebo (0.5 kcal, 0 g protein/kg body wt, CNT, n = 7) immediately after cycling exercise (60-75% (V) over dotO(2peak), 60 min/day, 3 days/wk) each day for 8 wk at similar to 19 degrees C ambient temperature (T(a)) and similar to 43% relative humidity (RH). Before and after training, we measured PV, cardiac stroke volume (SV), and esophageal temperature (T(es)) during 20-min exercise at 60% of pretraining (V) over dotO(2peak) at 30 degrees C T(a) and 50% RH. Moreover, we determined the sensitivity of the chest sweat rate (Delta SR/Delta T(es)) and forearm vascular conductance (Delta FVC/Delta T(es)) in response to increased Tes during exercise. After training, PV increased by similar to 6% in Pro-CHO (P < 0.001), with an similar to 10% increase in SV during exercise (P < 0.001), but not in CNT (P > 0.07). Delta FVC/Delta T(es) increased by 80% and Delta SR/Delta T(es) by 18% in Pro-CHO (both P < 0.01) but not in CNT (P > 0.07). Moreover, we found a significant interactive effect of group X training on PV, SV, and Delta FVC/Delta T(es) (all P < 0.02) but with no significant effect of group (P > 0.4), suggesting that the supplement enhanced these responses to aerobic training. Thus postexercise protein-CHO supplementation during training caused PV expansion and facilitated thermoregulatory and cardiovascular adaptations, possibly providing a new training regimen for older men.


  • Protein and carbohydrate supplementation after exercise increases plasma volume and albumin content in older and young men

    Kazunobu Okazaki, Hideki Hayase, Takashi Ichinose, Hiroyuki Mitono, Tatsuya Doi, Hiroshi Nose

    JOURNAL OF APPLIED PHYSIOLOGY   107 ( 3 ) 770 - 779  2009.09

     View Summary

    Okazaki K, Hayase H, Ichinose T, Mitono H, Doi T, Nose H. Protein and carbohydrate supplementation after exercise increases plasma volume and albumin content in older and young men. J Appl Physiol 107: 770-779, 2009. First published July 9, 2009; doi: 10.1152/japplphysiol.91264.2008.-This study examined whether increased plasma volume (PV) and albumin content (Alb(cont)) in plasma for 23 h after exercise were attenuated in older subjects compared with in young adult subjects, and if this attenuation abated by supplementation with protein and carbohydrate (CHO) immediately after exercise. Eight moderately active older (similar to 68 yr) and 8 young (similar to 21 yr) men performed two trials: control (CNT) and Pro-CHO in which subjects consumed placebo (0.5 kcal, 0 g protein, 0.5 mg Na(+) in 3.2 ml total fluid volume/kg body wt) or protein and CHO mixture (3.2 kcal, 0.18 g protein, 0.5 mg Na(+) in 3.2 ml total fluid volume/kg body wt) supplementations, respectively, immediately after high-intensity interval exercise for 72 min [ 8 sets of 4 min at 70-80% peak oxygen consumption rate ((V) over dotO(2peak)) intermitted by 5 min at 20% (V) over dotO(2peak)]. PV, Albcont, and plasma globulin content (Glb(cont)) were measured before exercise, at the end of exercise, every hour from the 1st to the 5th hour after exercise, and at the 23rd hour after exercise. From 12 h before the start to the end of experiment, food intake was controlled to the age-matched recommended dietary allowances. We found that during the first 4 h after exercise in CNT, Albcont recovered less in the older than the young group by similar to 0.04 g/kg (P < 0.05), while it generally recovered more with Pro-CHO than CNT by similar to 0.09 and similar to 0.04 g/kg in the young and older group, respectively, accompanied by a greater increase in PV by similar to 1 and similar to 2 ml/kg, respectively, during the 23 h after exercise (P < 0.05). Glbcont remained constant throughout the experiment in both trials for both age groups. Thus the attenuated responses of Albcont and PV after exercise in older subjects were restored by protein and CHO supplementation immediately after exercise, similarly to young subjects.


  • Tea catechins enhance the mRNA expression of uncoupling protein 1 in rat brown adipose tissue

    Sachiko Nomura, Takashi Ichinose, Manabu Jinde, Yu Kawashima, Kaoru Tachlyashiki, Kazuhiko Imaizumi

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY   19 ( 12 ) 840 - 847  2008.12

     View Summary

    The aim of the present study was to determine whether the antiobesity effects of tea catechins (TCs) are associated with the expression of uncoupling protein 1 (UCP1) in brown adipose tissue (BAT). Male Sprague-Dawley rats were fed a high-fat (HF; 35% fat) diet for 5 weeks, then divided into four groups and fed an HF, HF with 0.5% TC (HFTC), normal-fat (NF; 5% fat) or NF with 0.5% TC (NFTC) diet for 8 weeks. At the end of the experimental period, perirenal and epididymal white adipose tissues (WATs) and interscapular BAT were isolated. The NFTC group had significantly lower perirenal WAT weights than the NF group (NF: 12.7 +/- 0.53 g; NFTC: 10.2 +/- 0.43 g; P <.01), but the HF and HFTC groups did not differ significantly. TC intake had no effects on epididymal WAT weights. The NFTC and HFTC groups had significantly lower BAT weights than the NF and HF groups, respectively. The NFTC group had significantly higher UCP1 mRNA levels in BAT than the NF group (NF: 0.35 +/- 0.02; NFTC: 0.60 +/- 0.11; P <.05), but the HF and HFTC groups did not differ significantly. Thus, TC intake in the context of the NF diet reduced perirenal WAT weight and up-regulated UCP1 mRNA expression in BAT. These results suggest that the suppressive effect of TC on body fat accumulation is associated with UCP1 expression in BAT. (c) 2008 Elsevier Inc. All rights reserved.


  • Acute hypoosmolality attenuates the suppression of cutaneous vasodilation with increased exercise intensity

    H Mitono, H Endoh, K Okazaki, T Ichinose, S Masuki, A Takamata, H Nose

    JOURNAL OF APPLIED PHYSIOLOGY   99 ( 3 ) 902 - 908  2005.09

     View Summary

    We examined the hypothesis that elevation of the body core temperature threshold for forearm skin vasodilation (THFVC) with increased exercise intensity is partially caused by concomitantly increased plasma osmolality Eight young male subjects, wearing a body suit perfused with warm water to maintain the mean skin temperature at 34 +/- 1 degrees C (ranges), performed 20-min cycle-ergometer exercise at 30% peak aerobic power ((V) over dot o(2 peak)) under isoosmotic conditions (C), and at 65% (V) over dot o(2 pea)k under isoosmotic (HEXIOS) and hypoosmotic (HEXLos) conditions. In HEXLos, hypoosmolality was attained by hypotonic saline infusion with DDAVP, a V-2 agonist, before exercise. P-osmol (mosmol/kgH(2)O) increased after the start of exercise in both H-EX trials (P < 0.01) but not in C. The average P-osmol at 5 and 10 min in HEXIos was higher than in C (P < 0.01), whereas that in HEXLos was lower than in HEXIos (P < 0.01). The change in THFVC was proportional to that in P-osmol in every subject for three trials. The change in THFVC per unit change in P-osmol (Delta THFVC/Delta P-osmol, degrees C center dot mosmol(-1)center dot kgH(2)O(-1)) was 0.064 +/- 0.012 when exercise intensity increased from C to HEXIOS, similar to 0.086 +/- 0.020 when P-osmol decreased from HEXIOS to HEXLOS (P > 0.1). Moreover, there were no significant differences in plasma volume, heart rate, mean arterial pressure, and plasma lactate concentration around THFVC between HEXIOS and HEXLOS (P > 0.1). Thus the increase in THFVC due to increased exercise intensity was at least partially explained by the concomitantly increased P-osmol.


  • Ten-day endurance training attenuates the hyperosmotic suppression of cutaneous vasodilation during exercise but not sweating

    T Ichinose, K Okazaki, S Masuki, H Mitono, M Chen, H Endoh, H Nose

    JOURNAL OF APPLIED PHYSIOLOGY   99 ( 1 ) 237 - 243  2005.07

     View Summary

    It is well known that hyperosmolality suppresses thermoregulatory responses and that plasma osmolality (P-osmol) increases with exercise intensity. We examined whether the decreased esophageal temperature thresholds for cutaneous vasodilation (THFVC) and sweating (THSR) after 10-day endurance training (ET) are caused by either attenuated increase in Posmol at a given exercise intensity or blunted sensitivity of hyperosmotic suppression. Nine young male volunteers exercised on a cycle ergometer at 60% peak oxygen consumption rate ((V) over dotg O-2 peak) for 1 h/day for 10 days at 30 degrees C. Before and after ET, thermoregulatory responses were measured during 20-min exercise at pretraining 70% (V) over dot O-2 peak in the same environment as during ET under isoosmotic or hyperosmotic conditions. Hyperosmolality by similar to 10 mosmol/kgH(2)O was attained by acute hypertonic saline infusion. After ET, (V) over dot O-2 peak and blood volume (BV) both increased by similar to 4% (P < 0.05), followed by a decrease in THFVC ( P < 0.05) but not by that in THSR. Although there was no significant decrease in Posmol at the thresholds after ET, the sensitivity of increase in THFVC at a given increase in P-osmol [Delta THFVC/ Delta P-osmol, degrees C center dot (mosmol/ kgH(2)O)(-1)], determined by hypertonic infusion, was reduced to 0.021 +/- 0.005 from 0.039 +/- 0.004 before ET (P < 0.05). The individual reductions in Delta THFVC/Delta P-osmol after ET were highly correlated with their increases in BV around THFVC ( r = - 0.89, P < 0.005). In contrast, there was no alteration in the sensitivity of the hyperosmotic suppression of sweating after ET. Thus the downward shift of THFVC after ET was partially explained by the blunted sensitivity to hyperosmolality, which occurred in proportion to the increase in BV.


  • ヒト運動時体温調節の特殊性

    能勢博, 岡崎和伸, 増木静江, 一之瀬貴

    臨床体温   21 ( 1 ) 3 - 15  2003

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Books and Other Publications

  • ティーボール基礎編. みんなのスポーツ 第34巻第1号, pp 6-8


    日本体育社, 東京  2012

  • ティーボール実践編. みんなのスポーツ 第34巻第3号, pp 25-27


    日本体育社, 東京  2012

  • ティーボールのすすめ 一之瀬貴 PART I ティーボールに親しもう, 7. ティーボールのためのストレッチング pp 34-37

    日本ティーボール協会, 吉村正, 荒川博, 丸山克俊

    ベースボール・マガジン社, 東京  2001

  • 勝つための野球術 生まれ変わるピッチング 一之瀬貴 Part 5 スポーツ・バイオメカニクスから学ぶ「これがピッチングだ」 pp 136-152

    稲尾和久, 吉村正

    新星出版社, 東京  2001

  • 勝つための野球術 生まれ変わるバッティング 一之瀬貴 Part 5 スポーツ・バイオメカニクスから学ぶ「これがバッティングだ」 pp 178-197

    荒川博, 吉村正

    新星出版社, 東京  2001

Research Projects

  • ヒトの茶カテキン摂取に伴う脂肪分解反応と全身持久力の改善効果に関する運動生理学研究(花王株式会社)

    Project Year :


  • 持久性スポーツの競技力向上を目的としたファットローディング法の開発

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  • 運動・ドーピング薬物・アルコール・香辛料・栄養素欠乏による白血球系細胞の応答

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    SD系雄性ラットに、1)亜鉛欠乏食で4週間飼育した時、2)香辛料の辛味成分(capsaicin: CAP, dihydrocapsaicin: DHC, allyl-isothiocyanate: AIT)の投与及び3)合成副腎皮質ホルモン(dexamethasone: DEX)の投与によって総白血球・リンパ球・単球・好中球・好酸球・好塩基球の各細胞数が如何に変動するかを検討した。総リンパ球はTリンパ球・Bリンパ球・NK細胞に分画・定量した。その結果、亜鉛欠乏によって総リンパ球数を除いて全ての細胞数が欠乏日数にほぼ比例して増加した。それに対し、CAP(3mg/kgBW)を投与すると、総リンパ球・Tリンパ球・Bリンパ球の各細胞数が有意仁低下し、総白血球・好中球の各細胞数が増加したが、好酸球・好塩基球・単級の細胞数は変動しなかった。この現象はDHC(3mg/kgBW)でも認められたが、その影響の強さはDHGがCAPより高かった。これらの現象はAIT(20mg/kgBW)の急性投与でも同様の結果が得られた。DEX(1mg/kgBW)を1回投与すると、投与8時間までは総白血球・総リンパ球・Tリンパ球・Bリンパ球


  • Evaluation System of Research Initiatives in Waseda University

    American Evaluation Association Annual Meeting, Denver CO, USA 

    Presentation date: 2014.10

  • Hyperosmotic inhibition of cutaneous vasodilation was suppressed by hypervolemia after endurance training in a warm environment

    Experimental Biology 2004, Washington DC, USA 

  • Enhanced sensitivity of cutaneous vasodilation and cardiac stroke volume after 10-day exercise training in humans

    Experimental Biology 2005, San Diego, USA 

  • Tea catechin intake enhances fat utilization during prolonged exercise after endurance training in humans

    Experimental Biology 2007, Washington DC, USA 

  • 文理融合プロジェクトの推進に向けた研究者の意識−アンケート調査に基づく分析−

    研究・技術計画学会 第25回年次学術大会, 東京 

  • A single fat loading in the recovery period after exercise training enhanced fat oxidation during prolonged exercise in humans

  • 文理連携プロジェクトの推進に向けた大学内マネジメントのあり方

    研究・技術計画学会 第26回年次学術大会, 宇部 

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