Updated on 2022/05/17

写真a

 
SUZUKI, Katsuhiko
 
Affiliation
Faculty of Sport Sciences, School of Sport and Sciences
Job title
Professor
Mail Address
メールアドレス
Profile
Professor, Cooperative Major in Advanced Health Sciences, Tokyo University of Agriculture and Technology and Waseda University (PhD course only: 2010-2013); Adjunct Professor, Institute of Biomedical Innovation, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Australia (2013-2014); President, International Society of Exercise and Immunology (ISEI) (2015-2017); Guest Editor, "Exercise and Inflammation" of Antioxidants (2017-2018).

Concurrent Post

  • Affiliated organization   Global Education Center

  • Faculty of Sport Sciences   Graduate School of Sport Sciences

Education

  •  
    -
    2002

    Hirosaki University   Graduate School, Division of Medicine   Department of Hygiene  

  •  
    -
    1999

    Hirosaki University   Faculty of Medicine   Medical doctor course  

  •  
    -
    1993

    Waseda University   Graduate School, Division of Human Sciences   Life Sciences  

  •  
    -
    1991

    Waseda University  

Degree

  • Waseda University   M.S. (Human Sciences)

  • Hirosaki University   Ph.D. (Medicine)

Research Experience

  • 2013
    -
     

    Professor, Faculty of Sport Sciences, Waseda University

  • 2008
    -
     

    Associate Professor, Faculty of Sport Sciences, Waseda University

  • 2003
    -
     

    Assistant Professor, Faculty of Human Sciences, Waseda University

  • 2002
    -
     

    Instructor, Hirosaki University School of Medicine

  • 1999
    -
    2001

    Internal Medicine, Hospital, International Medical Center of Japan

Professional Memberships

  • 2011
    -
    Now

    Japanese Society of Clinical Sports Medicine

  • 2008
    -
    Now

    The Japan Geriatrics Society

  • 2008
    -
    Now

    Japanese Society of Clinical Physiology

  • 2003
    -
    Now

    The Japanese Society of Complementary and Alternative Medicine

  • 2000
    -
    Now

    International Society of Exercise and Immunology

  • 1992
    -
    Now

    The Japanese Society of Physical Fitness and Sports Medicine

  •  
     
     

    Japanese Society of Exercise and Immunology

▼display all

 

Research Areas

  • General internal medicine

  • Sports sciences

  • Nutrition science and health science

Research Interests

  • Exercise, Lifestyle-induced diseases, Immunology, Preventive Medicine

Papers

  • Structure-Activity Relationship of Anthocyanidins as an Inhibitory Effect on Osteoclast Differentiation

    Narumi Hirata, Tsukasa Tominari, Ryota Ichimaru, Keita Taniguchi, Chiho Matsumoto, Kenta Watanabe, Michiko Hirata, Sihui Ma, Katsuhiko Suzuki, Florian M.W. Grundler, Chisato Miyaura, Masaki Inada

    BPB Reports   2 ( 1 ) 1 - 6  2019  [Refereed]

    DOI

  • The combination of cardiorespiratory fitness and muscular fitness, and prevalence of diabetes mellitus in middle-aged and older men: WASEDA’S Health Study

    Dong Wang, Susumu S. Sawada, Hiroki Tabata, Ryoko Kawakami, Tomoko Ito, Kumpei Tanisawa, Mitsuru Higuchi, Kaori Ishii, Koichiro Oka, Katsuhiko Suzuki, Shizuo Sakamoto

    BMC Public Health   22 ( 1 )  2022.12  [Refereed]

     View Summary

    Abstract

    Background

    Although the negative relationship between cardiorespiratory fitness (CRF) or muscular fitness and diabetes mellitus were respectively observed in many previous studies, there is still a lack of studies that include CRF and muscular fitness simultaneously. Therefore, this study aimed to investigate the relationship between the combination of CRF and muscular fitness and diabetes through a cross-sectional study. 

    Methods

    This study was part of WASEDA'S Health Study, a cohort study launched in 2014. We used a part of the baseline data collected for this study. Maximal exercise test using a cycle ergometer and leg extension power (LEP) test were respectively used to evaluate CRF and muscular fitness. Since LEP is affected by body weight, relative LEP (rLEP) which is LEP per body weight, was used as an index of muscular fitness. 796 men (56.5 ± 10.4 years old) who completed a medical examination and fitness tests, were divided into two groups based on CRF and rLEP, respectively. The prevalence of diabetes was collected based on a self-reported questionnaire or blood test. Odds ratios and 95% confidence intervals (CIs) for the prevalence of diabetes were obtained using logistic regression models while adjusting for age, body mass index, exercise habits, family history of diabetes, smoking habits, and drinking habits.

    Results

    55 (7%) participants had diabetes. Compared to participants with lower CRF or rLEP, the odds ratio (95% CIs) of diabetes in those with higher CRF or rLEP was 0.46 (0.21–0.98) or 0.34 (0.16–0.74), respectively. Furthermore, using the lower CRF and lower rLEP group as the reference, the odds ratio (95% CIs) for the lower CRF and higher rLEP group was 0.32 (0.12–0.88), and higher CRF and higher rLEP group was 0.21 (0.07–0.63), after adjusting for potential confounding factors.

    Conclusions

    CRF and rLEP have independent and joint inverse associations with diabetes prevalence. In addition, participants with high CRF and high rLEP had a lower prevalence of diabetes compared to those with only high CRF or only high rLEP.

    DOI

  • Protective Roles of Exercise and Nutritional Factors for Immune System During Delta Variant-COVID-19 Outbreaks: Evidence Review and Practical Recommendations

    Behzad Taati, Seyed Mojtaba Paydar Ardakani, Katsuhiko Suzuki, Masoumeh Sadat Modaresi, Majid Taati Moghadam, Behnam Roozbeh

    Iranian Journal of Medical Microbiology   16 ( 3 ) 178 - 185  2022.05  [Refereed]

    DOI

  • Effects of Multicomponent Exercise Training Program on Biochemical and Motor Functions in Patients with Alzheimer’s Dementia

    Oussama Gaied Chortane, Raouf Hammami, Sofiene Amara, Sabri Gaied Chortane, Katsuhiko Suzuki, Rafael Oliveira, Hadi Nobari

    Sustainability   14 ( 7 ) 4112 - 4112  2022.03  [Refereed]

     View Summary

    The aim of this study was to examine the effects of a multicomponent exercise training program on motor function and biochemical markers in patients with Alzheimer’s-type dementia. Twenty patients with Alzheimer disease, divided into the intervention group (IG; aged 84 ± 3.1 years) and the control group (CG; aged 86 ± 2.6 years) were included in this study. The intervention group was enrolled into an exercise training program for three months (two sessions of 60 min per week). The CG was instructed to follow their daily rhythm of life (e.g., rest, reading) without a physical training program. After 3 months of participation in a multicomponent exercise program, gait speed, balance and walking parameters were all improved in the intervention group as measured with the Berg Balance Scale, the Tinetti test, the 6-min walking test and the timed up and go test (p < 0.05 for all; percentage range of improvements: 3.17% to 53.40%), except the walking while talking test, and biochemical parameters were not affected (p > 0.05). Our results demonstrate that exercise improves postural control, aerobic capacity and mobility functions in patients with Alzheimer disease. Physical exercise is a safe and effective method for treating physical disorders in patients with Alzheimer’s disease and can easily be integrated in various programs for the management of Alzheimer disease.

    DOI

  • Oxidative Stress and Inflammatory Status in COVID-19 Outpatients: A Health Center-Based Analytical Cross-Sectional Study

    Sahar Golabi, Sheyda Ghasemi, Maryam Adelipour, Reza Bagheri, Katsuhiko Suzuki, Alexei Wong, Maryam Seyedtabib, Mahshid Naghashpour

    Antioxidants   11 ( 4 ) 606 - 606  2022.03  [Refereed]

     View Summary

    The antioxidant system can be critical in reducing exacerbated inflammation in COVID-19. This study compared the antioxidant and inflammatory responses between COVID-19 outpatients and seemingly healthy individuals. This descriptive-analytical cross-sectional study was conducted on 53 COVID-19 outpatients and 53 healthy individuals as controls. The serum concentrations of amyloid A (SAA), total antioxidant capacity (TAC), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured and compared between COVID-19 patients and controls using the independent sample t-test before and after controlling for dietary supplement use. A generalized estimating equation (GEE) regression model, limited to COVID-19 patients, was used to evaluate the odds ratios (ORs) and 95% confidence intervals (95% CIs) of disease symptoms on days 1, 7, 14, 21, and 28 after the disease onset. Serum concentrations of SOD (p ≤ 0.001) and GPx (p = 0.001) were significantly higher in COVID-19 patients than in controls before adjustment for dietary supplement use. GPx remained significantly higher among COVID-19 patients than in controls after adjustment for all dietary supplements (p = 0.005). Moreover, serum concentrations of GPx (p = 0.003), SOD (p = 0.022), and TAC (p = 0.028) remained significantly higher among COVID-19 patients than in controls after adjustment for vitamin D supplementation. This study showed higher GPx in COVID-19 outpatients than in controls after adjustment for dietary supplement use. Moreover, elevated SOD, GPx, and TAC concentrations were shown in COVID-19 outpatients compared to controls after adjusting for vitamin D supplementation. These results may provide a useful therapeutic target for treating oxidative stress in COVID-19 disease, which may help ameliorate the pandemic.

    DOI

  • Short-Term Effects of Low-Fat Chocolate Milk on Delayed Onset Muscle Soreness and Performance in Players on a Women’s University Badminton Team

    Maryam Molaeikhaletabadi, Reza Bagheri, Mohammad Hemmatinafar, Javad Nemati, Alexei Wong, Michael Nordvall, Maryam Namazifard, Katsuhiko Suzuki

    International Journal of Environmental Research and Public Health   19 ( 6 ) 3677 - 3677  2022.03  [Refereed]

    Authorship:Corresponding author

     View Summary

    This study investigated the short-term effects of low-fat chocolate milk (LFCM) consumption on delayed onset muscle soreness (DOMS) and performance in female badminton players. Seven female badminton players (23 ± 1 years; height: 163.8 ± 4.1 cm; body mass: 58.7 ± 0.9 kg) were randomly assigned to 1 week of LFCM (500 mL) or placebo (water, 500 mL) consumption in a crossover design. Participants consumed LFCM or water immediately after each training session during the 1-week intervention. Performance variables (aerobic power, anaerobic power, agility, explosive power, and maximum handgrip strength) were assessed at two separate time points: pre and post-intervention (after 1 week). In addition, the Visual Analogue Scale (VAS) was used to assess DOMS before, immediately after, and at 24 and 48 h after each training session. There were significant time effects for aerobic power, upper body explosive power, minimum anaerobic power, and time to exhaustion (TTE), which significantly increased after LFCM consumption (p < 0.05). Moreover, relative and maximum lower body power significantly (p < 0.05) increased, while rating of perceived exertion (RPE) as well as DOMS in lower extremity muscles immediately after exercise significantly decreased after LFCM consumption compared to placebo (p < 0.05). There were no significant changes in maximum anaerobic power, agility, and maximum handgrip strength (p > 0.05). LFCM, as a post-exercise beverage, may help speed recovery in female badminton players leading to increased aerobic, anaerobic, and strength performance indices, increased TTE, and decreased muscle soreness and RPE.

    DOI

  • The effects of different temperatures of post-exercise protein-containing drink on gastric motility and energy intake in healthy young men

    Kyoko Fujihira, Yuka Hamada, Miki Haramura, Katsuhiko Suzuki, Masashi Miyashita

    British Journal of Nutrition   127 ( 5 ) 782 - 790  2022.03  [Refereed]  [International journal]

     View Summary

    Abstract

    The present study examined the effects of different temperatures of protein-containing drink after exercise on subsequent gastric motility and energy intake in healthy young men. Twelve healthy young men completed three, 1-d trials in a random order. In all trials, the subjects ran on a treadmill for 30 min at 80% of maximum heart rate. In exercise + cold drink (2°C) and exercise + hot drink (60°C) trials, the subjects consumed 300 ml of protein-containing drink (0·34 MJ) at 2°C or 60°C over a 5-min period after exercise. In the exercise (i.e. no preload) trial, the subjects sat on a chair for 5 min after exercise. Then, the subjects sat on a chair for 30 min to measure their gastric motility with an ultrasound imaging system in all trials. Thereafter, the subjects consumed a test meal until they felt comfortably full. Energy intake in the exercise + hot drink trial was 14 % and 15 % higher than the exercise (P = 0·046, 95% CI 4·010, 482·538) trial and exercise + cold drink (P = 0·001, 95% CI 160·089, 517·111) trial, respectively. The frequency of the gastric contractions in the exercise + hot drink trial was higher than the exercise (P = 0·023) trial and exercise + cold drink (P = 0·007) trial. The total frequency of gastric contractions was positively related to energy intake (r = 0·386, P = 0·022). These findings demonstrate that consuming protein-containing drink after exercise at 60°C increases energy intake and that this increase may be related to the modulation of the gastric motility.

    DOI PubMed

  • Effects of a New Form of Resistance-Type High-Intensity Interval Training on Cardiac Structure, Hemodynamics, and Physiological and Performance Adaptations in Well-Trained Kayak Sprint Athletes

    Mohsen Sheykhlouvand, Hamid Arazi, Todd A. Astorino, Katsuhiko Suzuki

    Frontiers in Physiology   13  2022.03  [Refereed]

     View Summary

    This study examined the effects of a resistance-type high-intensity interval training (RHIIT) matched with the lowest velocity that elicited O2peak (100% vO2peak) in well-trained kayak sprint athletes. Responses in cardiac structure and function, cardiorespiratory fitness, anaerobic power, exercise performance, muscular strength, and hormonal adaptations were examined. Male kayakers (n = 24, age: 27 ± 4 years) were randomly assigned to one of three 8-wk conditions (N = 8): (RHIIT) resistance training using one-armed cable row at 100% vO2peak; paddling-based HIIT (PHIIT) six sets of paddling at 100% vO2peak; or controls (CON) who performed six sessions including 1-h on-water paddling/sessions at 70–80% maximum HR per week. Significant increases (p < 0.05) in O2peak, vO2peak, maximal cardiac output, resting stroke volume, left ventricular end-systolic dimension, 500-m paddling performance were seen pre- to post-training in all groups. Change in O2peak in response to PHIIT was significantly greater (p = 0.03) compared to CON. Also, 500-m paddling performance changes in response to PHIIT and RHIIT were greater (p = 0.02, 0.05, respectively) than that of CON. Compared with pre-training, PHIIT and RHIIT resulted in significant increases in peak and average power output, maximal stroke volume, end-diastolic volume, ejection fraction, total testosterone, testosterone/cortisol ratio, and 1,000-m paddling performance. Also, the change in 1,000-m paddling performance in response to PHIIT was significantly greater (p = 0.02) compared to that of CON. Moreover, maximum strength was significantly enhanced in response to RHIIT pre- to post-training (p < 0.05). Overall, RHIIT and PHIIT similarly improve cardiac structure and hemodynamics, physiological adaptations, and performance of well-trained kayak sprint athletes. Also, RHIIT enhances cardiorespiratory fitness and muscular strength simultaneously.

    DOI

  • Effects of Different Types of Exercise on Kidney Diseases

    Hamid Arazi, Majid Mohabbat, Payam Saidie, Akram Falahati, Katsuhiko Suzuki

    Sports   10 ( 3 ) 42 - 42  2022.03  [Refereed]

    Authorship:Corresponding author

     View Summary

    The effects of exercise on kidney function have been studied for more than three decades. One of the most common health issues among patients with chronic kidney disease (CKD) is a lack of physical activity, which leads to a low exercise capacity in these patients. The majority of maintenance hemodialysis (MHD) patients do not exercise at all. At each stage of dialysis, patients lose 10–12 g of their amino acids through blood sampling. Dialysis also leads to increased cortisol and circadian rhythm sleep disorders in hemodialysis (HD) patients. Studies have also reported higher C-reactive protein levels in HD patients, which causes arterial stiffness. Exercise has a variety of health benefits in these patients, including improved blood pressure control, better sleep, higher physical function, and reduced anxiety and depression. On the other hand, it should be noted that intense exercise has the potential to progress KD, especially when conducted in hot weather with dehydration. This review aimed to investigate the effects of different types of exercise on kidney disease and provide exercise guidelines. In conclusion, moderate-intensity and long-term exercise (for at least a 6-month period), with consideration of the principles of exercise (individualization, intensity, time, etc.), can be used as an adjunctive treatment strategy in patients undergoing dialysis or kidney transplantation.

    DOI

  • Combined Effects of Exercise Training and Nutritional Supplementation in Cancer Patients in the Context of the COVID-19: A Perspective Study

    Mahdieh Molanouri Shamsi, Alieh Vahed, AmirHossin Ahmadi Hekmatikar, Katsuhiko Suzuki

    Frontiers in Nutrition   9  2022.03  [Refereed]

     View Summary

    The 2019 coronavirus (COVID-19) epidemic, has caused unprecedented global social and economic impacts and many deaths. Many risk factors have been identified in the progression of COVID-19 to severe and critical stages, and it is shown that the coronavirus appears more severely in people with cancer. Pro-inflammatory status and weakened immune system due to cancer-related treatments can be determinants in the immune system’s response to the coronavirus in these patients. Higher physical activity levels are associated with lower hospitalization rates and mortality in COVID-19. Also, regular exercise training can improve immune system responses, modulate inflammatory responses, and improve psychological parameters in cancer patients. The interactive effects of nutritional supplements on immune responses and anti-inflammatory status have been shown in some studies. The purpose of this perspective article was to investigate the interaction between dietary supplementation and regular physical exercise in controlling risk factors associated with coronavirus in cancer patients. In addition to appropriate dietary habits, some nutritional supplements, especially vitamin D, have been shown to improve the immune system’s response against COVID-19 and cancer. Using lifestyle strategies such as regular physical activity and intake of functional compounds as supplements can be effective in treatment outcomes, quality of life, and overall survival in cancer patients. We proposed that combining dietary supplements and exercise training in cancer patients can boost immune responses against COVID-19 and probably improve vaccine responses. Angiotensin (ANG)-(1-7) Mas receptor axis can probably activate following exercise training and vitamin D combination. And can prevent pulmonary injury, hematological alterations, and hyperinflammatory state in COVID-19.

    DOI

  • Thermal dysregulation in patients with multiple sclerosis during SARS-CoV-2 infection. The potential therapeutic role of exercise

    Omid Razi, Bakhtyar Tartibian, Ana Maria Teixeira, Nastaran Zamani, Karuppasamy Govindasamy, Katsuhiko Suzuki, Ismail Laher, Hassane Zouhal

    Multiple Sclerosis and Related Disorders   59   103557 - 103557  2022.03  [Refereed]

    DOI

  • Functional and Psychological Changes after Exercise Training in Post-COVID-19 Patients Discharged from the Hospital: A PRISMA-Compliant Systematic Review

    Amir Hossein Ahmadi Hekmatikar, João Batista Ferreira Júnior, Shahnaz Shahrbanian, Katsuhiko Suzuki

    International Journal of Environmental Research and Public Health   19 ( 4 ) 2290 - 2290  2022.02  [Refereed]

    Authorship:Corresponding author

     View Summary

    Millions of people worldwide are infected with COVID-19, and COVID-19 survivors have been found to suffer from functional disabilities and mental disorders such as depression and anxiety. This is a matter of concern because COVID-19 is still not over. Because reinfection is still possible in COVID-19 survivors, decreased physical function and increased stress and anxiety can lower immune function. However, the optimal exercise intensity and volume appear to remain unknown. Therefore, the current systematic review aimed to evaluate the effect of resistance or aerobic exercises in post-COVID-19 patients after hospital discharge. We conducted searches in the Scopus, SciELO, PubMed, Web of Science, Science Direct, and Google Scholar databases. Studies that met the following criteria were included: (i) English language, (ii) patients with COVID-19 involved with resistance or aerobic exercise programs after hospital discharge. Out of 381 studies reviewed, seven studies met the inclusion criteria. Evidence shows that exercise programs composed of resistance exercise (e.g., 1–2 sets of 8–10 repetitions at 30–80% of 1RM) along with aerobic exercise (e.g., 5 to 30 min at moderate intensity) may improve the functional capacity and quality of life (reduce stress and mental disorders) in post-COVID-19 patients. In addition, only one study reported reinfection of three subjects involved with the exercise program, suggesting that exercise programs may be feasible for the rehabilitation of the patients. A meta-analysis was not conducted because the included studies have methodological heterogeneities, and they did not examine a control group. Consequently, the results should be generalized with caution.

    DOI

  • A Guide to Different Intensities of Exercise, Vaccination, and Sports Nutrition in the Course of Preparing Elite Athletes for the Management of Upper Respiratory Infections during the COVID-19 Pandemic: A Narrative Review

    Hamid Agha-Alinejad, Amir Hossein Ahmadi Hekmatikar, Ruheea Taskin Ruhee, Mahdieh Molanouri Shamsi, Masoud Rahmati, Kayvan Khoramipour, Katsuhiko Suzuki

    International Journal of Environmental Research and Public Health   19 ( 3 ) 1888 - 1888  2022.02  [Refereed]

     View Summary

    Elite athletes use high-intensity training to maintain their fitness level. However, intense training can harm the immune system, making athletes suspectable to COVID-19 and negatively affecting their performance. In addition, the diet of athletes should be appreciated more as it is another influencer of the immune system, especially during the COVID 19 pandemic. The other important issue elite athletes face currently is vaccination and its possible intervention with their training. The present study attempts to discuss the impact of different training intensities, nutritional strategies, and vaccination on the immune system function in elite athletes. To this end, Scopus, ISC, PubMed, Web of Science, and Google Scholar databases were searched from 1988 to 2021 using the related keywords. The results of our review showed that although high-intensity exercise can suppress the immune system, elite athletes should not stop training in the time of infection but use low- and moderate-intensity training. Moderate-intensity exercise can improve immune function and maintain physical fitness. In addition, it is also better for athletes not to undertake high-intensity training at the time of vaccination, but instead perform moderate to low-intensity training. Furthermore, nutritional strategies can be employed to improve immune function during high-intensity training periods.

    DOI

  • Effects of oral cystine and glutamine on exercise-induced changes in gastrointestinal permeability and damage markers in young men

    Yusei Tataka, Miki Haramura, Yuka Hamada, Miho Ono, Sakiko Toyoda, Toshiyuki Yamada, Ayano Hiratsu, Katsuhiko Suzuki, Masashi Miyashita

    European Journal of Nutrition    2022.02  [Refereed]

     View Summary

    Abstract

    Purpose

    Although acute prolonged strenuous exercise has been shown to increase markers of gastrointestinal permeability and damage, little is known regarding the efficacy of nutritional supplement interventions on the attenuation of exercise-induced gastrointestinal syndrome. This study addressed the effects of oral amino acid supplementation on markers of gastrointestinal permeability and damage in response to exercise.

    Methods

    Sixteen active men aged 22.7 ± 2.6 years (mean ± standard deviation) completed placebo or cystine and glutamine supplementation trials in random order. Participants received either a placebo or cystine and glutamine supplements, three times a day for 5 days, separated by a 2-week washout period. On day 6, participants took their designated supplements 30 min before running at a speed corresponding to 75% of maximal oxygen uptake for 1 h, followed by a 4-h rest period. Blood samples were collected pre-exercise, immediately post-exercise, 30 min post-exercise, and 1, 2 and 4 h post-exercise on day 6. The plasma lactulose to mannitol ratio (L:M) and plasma intestinal fatty acid-binding protein (I-FABP) were used as markers of gastrointestinal permeability and damage, respectively.

    Results

    Plasma L:M (linear mixed model, coefficient ± standard error: − 0.011 ± 0.004, P = 0.0090) and changes (i.e., from pre-exercise) in plasma I-FABP (linear mixed model, − 195.3 ± 65.7 coefficient ± standard error (pg/mL), P = 0.0035) were lower in the cystine and glutamine supplementation trial than in the placebo trial.

    Conclusion

    Oral cystine and glutamine supplementation attenuated the markers of gastrointestinal permeability and damage after 1 h of strenuous running in young men.

    Trial registration number

    UMIN000026008.

    Date of registration

    13 December 2018.

    DOI

  • Association Between Dietary Patterns and Different Metabolic Phenotypes in Japanese Adults: WASEDA'S Health Study

    Kumpei Tanisawa, Tomoko Ito, Ryoko Kawakami, Chiyoko Usui, Takuji Kawamura, Katsuhiko Suzuki, Shizuo Sakamoto, Kaori Ishii, Isao Muraoka, Koichiro Oka, Mitsuru Higuchi

    Frontiers in Nutrition   9  2022.01  [Refereed]

     View Summary

    Although many studies have reported that a posteriori dietary pattern is associated with metabolic health, there is little evidence of an association between dietary patterns and different metabolic phenotypes. The present study aimed to examine the association between major dietary patterns and different metabolic phenotypes (metabolically healthy non-obese [MHNO], metabolically unhealthy non-obese [MUNO], metabolically healthy obese [MHO], and metabolically unhealthy obese [MUO]) in middle-aged and elderly Japanese adults. This cross-sectional study enrolled 2,170 Japanese adults aged ≥40 years. The four different metabolic phenotypes were determined based on the presence of obesity, abdominal obesity, hypertension, hyperglycemia, and dyslipidemia. The major dietary patterns were determined using principal component analysis based on energy-adjusted food intake. Two dietary patterns were identified: the healthy dietary pattern, which was characterized by a high intake of vegetables, fruits, potatoes, soy products, mushrooms, seaweeds, and fish; and the alcohol dietary pattern, which was characterized by a high intake of alcoholic beverages, liver, chicken, and fish. The healthy dietary pattern was associated with the MHNO and MHO phenotypes (MUNO and MUO as reference groups, respectively), and the multivariate-adjusted odds ratios (ORs) (95% confidence intervals [CIs]) in the highest quartile of healthy dietary pattern score with the lowest quartile as the reference category were 2.10 (1.40–3.15) and 1.86 (1.06–3.25), respectively. Conversely, the alcohol dietary pattern was inversely associated with the MHNO and MHO phenotypes, while the multivariate-adjusted ORs (95% CIs) in the highest quartile of the alcohol dietary pattern score with the lowest quartile as the reference category were 0.63 (0.42–0.94) and 0.45 (0.26–0.76), respectively. There were no significant interactions between sex and healthy/alcohol dietary patterns in the prevalence of the MHNO and MHO phenotypes. In conclusion, the present study's findings suggest that major dietary patterns are associated with different metabolic phenotypes in middle-aged and elderly Japanese adults. These findings provide useful evidence for maintaining metabolic health through diet regardless of obesity status.

    DOI

  • Effects of 8 Weeks of High-Intensity Interval Training and Spirulina Supplementation on Immunoglobin Levels, Cardio-Respiratory Fitness, and Body Composition of Overweight and Obese Women

    Hadi Nobari, Elham Eyni Gandomani, Jalil Reisi, Reyhaneh Vahabidelshad, Katsuhiko Suzuki, Stella Lucia Volpe, Jorge Pérez-Gómez

    Biology   11 ( 2 ) 196 - 196  2022.01  [Refereed]

     View Summary

    Our study examined the effect of 8 weeks of high-intensity interval training (HIIT) and spirulina supplementation on the humoral immunity, cardio-respiratory fitness, and body composition of overweight and obese women. Thirty sedentary women (height: 161.7 ± 2.8 cm, body mass: 75.8 ± 8.4 kg, body mass index [BMI]: 28.8 ± 2.5 kg/m2, age: 25.1 ± 6.7 years) were divided into three groups: placebo with HIIT group, spirulina group (SG), or combined group (CG). Exercise groups performed HIIT for 8 weeks, with three sessions per week and four to seven repetitions in each session of 30 s running and 30 s walking; the intensity was established at 90% of the maximum heart rate. Supplementation groups received 6 g of spirulina powder per day. Fasting blood samples were collected before and after 8 weeks to determine the concentrations of immunoglobulins (IgA and IgG). There was a significant group-by-time interaction for fat free mass (FFM; p = 0.001, f = 8.52, ηp2 = 0.39) and IgA (p = 0.036, f = 3.86, ηp2 = 0.22). The post hoc analysis revealed that CG reduced FFM significantly (p = 0.012, g = −0.55) after training. CG and SG showed significantly greater IgA concentrations after 8 weeks (p = 0.02, g = 0.70 and p = 0.001, g = 0.34, respectively). We conclude that spirulina supplementation with HIIT affects the body composition (lower FFM) but also boosts IgA, which plays an important role in the immune system.

    DOI

  • Effects of exercise training on depression and anxiety with changing neurotransmitters in methamphetamine long term abusers: A narrative review

    Hamid Arazi, Seyedeh Shiva Dadvand, Katsuhiko Suzuki

    Biomedical Human Kinetics   14 ( 1 ) 117 - 126  2022.01  [Refereed]

     View Summary

    Abstract

    Study aim: It is generally accepted that methamphetamine (MA) is a highly addictive psychostimulant which copies functions of certain neurotransmitters in the brain, and emits dopamine and serotonin in the brain. Frequent abuse of methamphetamine damages dopaminergic and serotonergic nerve endings at different sites of brain and also increases the anxiety and depression. Exercise seems to reverse physiological and neurological damages due to previous MA dependents and help to reduce anxiety and depression in this population. The aim is to determine the effect of exercise training on neurotransmitters and rate of depression and anxiety in chronic methamphetamine abusers based on a literature review.

    Materials and methods: The present study investigated published articles in five computerized databases including Magiran, Google scholars, SID, PubMed and Scopus from 2011 until 2020. The searched keywords included sports, neurotransmitter, methamphetamine, depression, anxiety and treatment. The obtained results were described using texts, table and figure.

    Results: According to the results, physical activity and exercise significantly increased blood serotonin and dopamine levels and significantly decreased the depression and anxiety.

    Conclusion: Different evidences suggested that physical activity and exercise as positive responses and adaptations might influence on circulatory levels of two neurotransmitters (serotonin and dopamine) in methamphetamine addicts, decrease anxiety and depression, and improve preparation in previous MA dependents. In the rehabilitation period, the overall improvement in previous MA dependents might significantly increase. Exercise training can improve the physical and mental state of people addicted to methamphetamine as a non-drug therapy to promote health. However, more research is necessary to support this conclusion.

    DOI

  • Ultra-Processed Food Consumption and Adult Mortality Risk: A Systematic Review and Dose–Response Meta-Analysis of 207,291 Participants

    Wanich Suksatan, Sajjad Moradi, Fatemeh Naeini, Reza Bagheri, Hamed Mohammadi, Sepide Talebi, Sanaz Mehrabani, Mohammad ali Hojjati Kermani, Katsuhiko Suzuki

    Nutrients   14 ( 1 ) 174 - 174  2021.12  [Refereed]

    Authorship:Corresponding author

     View Summary

    We performed a systematic review and dose–response meta-analysis of observational studies assessing the association between UPF consumption and adult mortality risk. A systematic search was conducted using ISI Web of Science, PubMed/MEDLINE, and Scopus electronic databases from inception to August 2021. Data were extracted from seven cohort studies (totaling 207,291 adults from four countries). Using a random-effects model, hazard ratios (HR) of pooled outcomes were estimated. Our results showed that UPF consumption was related to an enhanced risk of all-cause mortality (HR = 1.21; 95% CI: 1.13, 1.30; I2 = 21.9%; p < 0.001), cardiovascular diseases (CVDs)-cause mortality (HR = 1.50; 95% CI: 1.37, 1.63; I2 = 0.0%; p < 0.001), and heart-cause mortality (HR = 1.66; 95% CI: 1.50, 1.85; I2 = 0.0%; p = 0.022), but not cancer-cause mortality. Furthermore, our findings revealed that each 10% increase in UPF consumption in daily calorie intake was associated with a 15% higher risk of all-cause mortality (OR = 1.15; 95% CI: 1.09, 1.21; I2 = 0.0%; p < 0.001). The dose–response analysis revealed a positive linear association between UPF consumption and all-cause mortality (Pnonlinearity = 0.879, Pdose–response = p < 0.001), CVDs-cause mortality (Pnonlinearity = 0.868, Pdose–response = p < 0.001), and heart-cause mortality (Pnonlinearity = 0.774, Pdose–response = p < 0.001). It seems that higher consumption of UPF is significantly associated with an enhanced risk of adult mortality. Despite this, further experimental studies are necessary to draw a more definite conclusion.

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  • Pharmacological Inhibition of CCR2 Signaling Exacerbates Exercise-Induced Inflammation Independently of Neutrophil Infiltration and Oxidative Stress

    Takaki Tominaga, Jiapeng Huang, Katsuhiko Suzuki

    Immuno   2 ( 1 ) 26 - 39  2021.12  [Refereed]

     View Summary

    Although exercise-induced humoral factors known as exerkines benefit systemic health, the role of most exerkines has not been investigated. Monocyte chemoattractant protein-1 (MCP-1) is a representative chemokine whose circulating concentrations increase after exercise, and it is one of the exerkines. MCP-1 is a ligand for CC chemokine receptor 2 (CCR2), which is expressed on monocytes, macrophages, and muscle cells. However, there is no information on the role of CCR2 signaling in exercise. Therefore, to investigate the research question, we administrated CCR2 antagonist or PBS to mice to inhibit CCR2 signaling before and after exercise. Our results showed that CCR2 signaling inhibition promoted exercise-induced macrophage infiltration and inflammation 24 h after exercise in muscle. CCR2 signaling inhibition also exacerbated exercise-induced inflammation immediately after exercise in muscle. However, neutrophil infiltration and oxidative stress had no contribution to exercise-induced inflammation by CCR2 signaling inhibition. CCR2 signaling inhibition also exacerbated exercise-induced inflammation immediately after exercise in kidney, liver, and adipose tissues. To summarize, pharmacological inhibition of CCR2 signaling exacerbated exercise-induced inflammation independently of neutrophil infiltration and oxidative stress.

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  • An Overview on How Exercise with Green Tea Consumption Can Prevent the Production of Reactive Oxygen Species and Improve Sports Performance

    Hadi Nobari, Saber Saedmocheshi, Linda H. Chung, Katsuhiko Suzuki, Marcos Maynar-Mariño, Jorge Pérez-Gómez

    International Journal of Environmental Research and Public Health   19 ( 1 ) 218 - 218  2021.12  [Refereed]

     View Summary

    Free radicals are reactive products that have multiple effects on the human body. Endogenous and exogenous antioxidants manage the overproduction of free radicals. However, an imbalance between free radicals and antioxidant factors causes oxidative stress. Exercise and physical activity are factors that increase oxidative stress and disrupts the body’s homeostasis. Intensity and duration of training, training characteristics, and fitness level can have positive or negative effects on oxidative stress. Green tea consumption is recommended for the prevention of a variety of diseases, health maintenance, and weight loss. The effectiveness of green tea is primarily due to the presence of catechins and polyphenols, specifically (–)-epigallocatechin-3-gallate, which has antioxidant and anti-inflammatory properties based on clinical and animal studies. This review investigates the effect of green tea exercise and their interactive effects on free radicals and sports improvement.

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  • Dietary Supplementation for Attenuating Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness in Humans

    Yoko Tanabe, Naoto Fujii, Katsuhiko Suzuki

    Nutrients   14 ( 1 ) 70 - 70  2021.12  [Refereed]

    Authorship:Corresponding author

     View Summary

    Dietary supplements are widely used as a nutritional strategy to improve and maintain performance and achieve faster recovery in sports and exercise. Exercise-induced muscle damage (EIMD) is caused by mechanical stress and subsequent inflammatory responses including reactive oxygen species and cytokine production. Therefore, dietary supplements with anti-inflammatory and antioxidant properties have the potential to prevent and reduce muscle damage and symptoms characterized by loss of muscle strength and delayed-onset muscle soreness (DOMS). However, only a few supplements are considered to be effective at present. This review focuses on the effects of dietary supplements derived from phytochemicals and listed in the International Olympic Committee consensus statement on muscle damage evaluated by blood myofiber damage markers, muscle soreness, performance, and inflammatory and oxidative stress markers. In this review, the effects of dietary supplements are also discussed in terms of study design (i.e., parallel and crossover studies), exercise model, and such subject characteristics as physical fitness level. Future perspectives and considerations for the use of dietary supplements to alleviate EIMD and DOMS are also discussed.

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  • Recent Progress in Applicability of Exercise Immunology and Inflammation Research to Sports Nutrition

    Katsuhiko Suzuki

    Nutrients   13 ( 12 ) 4299 - 4299  2021.11  [Refereed]

    Authorship:Lead author

     View Summary

    This article focuses on how nutrition may help prevent and/or assist with recovery from the harmful effects of strenuous acute exercise and physical training (decreased immunity, organ injury, inflammation, oxidative stress, and fatigue), with a focus on nutritional supplements. First, the effects of ketogenic diets on metabolism and inflammation are considered. Second, the effects of various supplements on immune function are discussed, including antioxidant defense modulators (vitamin C, sulforaphane, taheebo), and inflammation reducers (colostrum and hyperimmunized milk). Third, how 3-hydroxy-3-methyl butyrate monohydrate (HMB) may offset muscle damage is reviewed. Fourth and finally, the relationship between exercise, nutrition and COVID-19 infection is briefly mentioned. While additional verification of the safety and efficacy of these supplements is still necessary, current evidence suggests that these supplements have potential applications for health promotion and disease prevention among athletes and more diverse populations.

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  • Moderate Intensity Aerobic Exercise Potential Favorable Effect Against COVID-19: The Role of Renin-Angiotensin System and Immunomodulatory Effects

    Hamid Arazi, Akram Falahati, Katsuhiko Suzuki

    Frontiers in Physiology   12  2021.11  [Refereed]

     View Summary

    The coronavirus disease (COVID-19) pandemic is caused by a novel coronavirus (CoV) named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). As the angiotensin converting enzyme 2 (ACE2) is the cellular receptor of SARS-CoV-2, it has a strong interaction with the renin angiotensin system (RAS). Experimental studies have shown that the higher levels of ACE2 or increasing ACE2/ACE1 ratio improve COVID-19 outcomes through lowering inflammation and death. Aerobic moderate intensity physical exercise fights off infections by two mechanisms, the inhibition of ACE/Ang II/AT1-R pathway and the stimulation of ACE2/Ang-(1–7)/MasR axis. Exercise can also activate the anti-inflammatory response so that it can be a potential therapeutic strategy against COVID-19. Here, we summarize and focus the relation among COVID-19, RAS, and immune system and describe the potential effect of aerobic moderate intensity physical exercise against CoV as a useful complementary tool for providing immune protection against SARS-CoV-2 virus infection, which is a novel intervention that requires further investigation.

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  • The Effects of TRX Suspension Training Combined with Taurine Supplementation on Body Composition, Glycemic and Lipid Markers in Women with Type 2 Diabetes

    Shohreh Samadpour Masouleh, Reza Bagheri, Damoon Ashtary-Larky, Neda Cheraghloo, Alexei Wong, Omid Yousefi Bilesvar, Katsuhiko Suzuki, Marefat Siahkouhian

    Nutrients   13 ( 11 ) 3958 - 3958  2021.11  [Refereed]

     View Summary

    Background: We aimed to investigate the effects of an 8-week total-body resistance exercise (TRX) suspension training intervention combined with taurine supplementation on body composition, blood glucose, and lipid markers in T2D females. Methods: Forty T2D middle-aged females (age: 53 ± 5 years, body mass = 84.3 ± 5.1 kg) were randomly assigned to four groups, TRX suspension training + placebo (TP; n = 10), TRX suspension training + taurine supplementation (TT; n = 10), taurine supplementation (T; n = 10), or control (C; n = 10). Body composition (body mass, body mass index (BMI), body fat percentage (BFP)), blood glucose (fasting blood sugar (FBS)), hemoglobin A1c (HbA1c), Insulin, and Insulin resistance (HOMA-IR), and lipid markers (low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglyceride (TG), and total cholesterol (TC)) were evaluated prior to and after interventions. Results: All three interventions significantly decreased body mass, BMI, and BFP with no changes between them for body mass and BMI; however, BFP changes in the TT group were significantly greater than all other groups. FBS was significantly reduced in TP and TT. Insulin concentrations’ decrement were significantly greater in all experimental groups compared to C; however, no between group differences were observed between TT, TP, and T. In regards to HOMA-IR, decreases in TT were significantly greater than all other groups TG, HbA1c, and LDL were reduced following all interventions. HDL values significantly increased only in the TT group, while TC significantly decreased in TP and TT groups. Changes in HbA1c, TG, HDL, and TC were significantly greater in the TT compared to all other groups. Conclusions: TRX training improved glycemic and lipid profiles, while taurine supplementation alone failed to show hypoglycemic and hypolipidemic properties. Notably, the synergic effects of TRX training and taurine supplementation were shown in HbA1c, HOMA-IR, TG, TC, HDL, and BFP changes. Our outcomes suggest that TRX training + taurine supplementation may be an effective adjuvant therapy in individuals with T2D.

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  • Effect of Green Tea Supplementation on Antioxidant Status in Adults: A Systematic Review and Meta-Analysis of Randomized Clinical Trials

    Niloufar Rasaei, Omid Asbaghi, Mahsa Samadi, Leila Setayesh, Reza Bagheri, Fatemeh Gholami, Neda Soveid, Krista Casazza, Alexei Wong, Katsuhiko Suzuki, Khadijeh Mirzaei

    Antioxidants   10 ( 11 ) 1731 - 1731  2021.10  [Refereed]

     View Summary

    It is well-established that green tea supplementation has antioxidant properties. However, whether green tea supplementation leads to oxidative stress reduction remains unclear, as clinical investigations on this subject have yielded inconsistent outcomes. Consequently, we aimed to determine the effects of green tea supplementation on oxidative stress in adults. A systematic search of English language publications up to 21 August 2021 was carried out in PubMed, Scopus, Embase, and ISI Web of Science, utilizing pertinent keywords. These searches included randomized controlled trials (RCTs) evaluating the relationship between green tea supplementation, malondialdehyde (MDA), and total antioxidant capacity (TAC) in adults. A random-effects model was used to estimate the weighted mean difference (WMD) and 95% confidence intervals (95% CI). Meta-regression and non-linear dose-response analyses were performed to investigate the association between the dosage of green tea (mg/day) and the duration of the intervention (weeks) with pooled effect size. Sixteen RCTs with seventeen arms including 760 participants met the inclusion criteria. Our results indicated that green tea supplementation had significant effects on TAC (weighted mean difference [WMD]: 0.20 mmol/L; 95% CI: 0.09, 0.30, p < 0.001) and significant heterogeneity between studies (I2 = 98.6%, p < 0.001), which was largely related to gender and body mass index (BMI). Subgroup analysis in TAC identified a significant relationship except with low dose supplementation and obese individuals. No relationship between MDA and green tea supplementation was observed in any subgroups; however, meta-regression analysis revealed a linear inverse association between the dosage and significant change in MDA (r = −2117.18, p = 0.017). Our outcomes suggest that green tea supplementation improves TAC and affects MDA based on the dose of the intervention in adults. Future RCTs with longer durations are needed to expand our findings.

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  • The Combined Effects of 6 Weeks of Jump Rope Interval Exercise and Dark Chocolate Consumption on Antioxidant Markers in Obese Adolescent Boys

    Babak Hooshmand Moghadam, Reza Bagheri, Matin Ghanavati, Fatemeh Khodadadi, Neda Cheraghloo, Alexei Wong, Michael Nordvall, Katsuhiko Suzuki, Fatemeh Shabkhiz

    Antioxidants   10 ( 11 ) 1675 - 1675  2021.10  [Refereed]

     View Summary

    Research has shown that both dark chocolate and exercise training may have favorable effects on antioxidant function in obese cohorts. However, their combined effect has not been established. We assessed the influences of six weeks of dark chocolate consumption combined with jump rope exercise on antioxidant markers in adolescent boys with obesity. Fifty adolescent boys with obesity (age = 15 ± 1 years) were randomly assigned into one of four groups; jump rope exercise + white chocolate consumption (JW; n = 13), jump rope exercise + dark chocolate consumption (JD; n = 13), dark chocolate consumption (DC; n = 12), or control (C; n = 12). Two participants dropped out of the study. Participants in JW and JD groups performed jump rope exercise three times per week for six weeks. Participants in the DC and JD groups consumed 30 g of dark chocolate containing 83% of cocoa during the same period. Serum concentrations of superoxide dismutase (SOD), total antioxidant capacity (TAC), glutathione peroxidase (GPx), and thiobarbituric acid reactive substances (TBARS) were evaluated prior to and after the interventions. All 3 intervention groups noted significant (p < 0.01) increases in serum concentrations of TAC, SOD, and GPx from baseline to post-test. In contrast, all intervention groups showed significantly reduced serum concentrations of TBARS from pre- to post-test (p ≤ 0.01). Bonferroni post hoc analysis revealed that post-test serum concentrations of TAC in the JD group were significantly greater than C (p < 0.001), DC (p = 0.010), and JW (p < 0.001) groups. In addition, post-test serum concentrations of SOD in the JD group were significantly greater than C group (p = 0.001). Post-test serum concentrations of GPx in the JD group were significantly greater than C (p < 0.001), DC (p = 0.021), and JW (p = 0.032) groups. The post-test serum concentrations of TBARS in the JD group was significantly lower than C (p < 0.001). No other significant between-group differences were observed. The current study provides evidence that dark chocolate consumption in combination with jump rope exercise is more efficient in improving antioxidant capacity than dark chocolate consumption or jump rope exercise alone among obese adolescent boys.

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  • Cardiac Oxidative Stress and the Therapeutic Approaches to the Intake of Antioxidant Supplements and Physical Activity

    Kosar Valaei, Shima Taherkhani, Hamid Arazi, Katsuhiko Suzuki

    Nutrients   13 ( 10 ) 3483 - 3483  2021.09  [Refereed]

     View Summary

    Reactive oxygen species (ROS) are strongly reactive chemical entities that include oxygen regulated by enzymatic and non-enzymatic antioxidant defense mechanisms. ROS contribute significantly to cell homeostasis in the heart by regulating cell proliferation, differentiation, and excitation-contraction coupling. When ROS generation surpasses the ability of the antioxidant defense mechanisms to buffer them, oxidative stress develops, resulting in cellular and molecular disorders and eventually in heart failure. Oxidative stress is a critical factor in developing hypoxia- and ischemia-reperfusion-related cardiovascular disorders. This article aimed to discuss the role of oxidative stress in the pathophysiology of cardiac diseases such as hypertension and endothelial dysfunction. This review focuses on the various clinical events and oxidative stress associated with cardiovascular pathophysiology, highlighting the benefits of new experimental treatments such as creatine supplementation, omega-3 fatty acids, microRNAs, and antioxidant supplements in addition to physical exercise

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  • An Overview of Physical Exercise and Antioxidant Supplementation Influences on Skeletal Muscle Oxidative Stress

    Shima Taherkhani, Kosar Valaei, Hamid Arazi, Katsuhiko Suzuki

    Antioxidants   10 ( 10 ) 1528 - 1528  2021.09  [Refereed]

     View Summary

    One of the essential injuries caused by moderate to high-intensity and short-duration physical activities is the overproduction of reactive oxygen species (ROS), damaging various body tissues such as skeletal muscle (SM). However, ROS is easily controlled by antioxidant defense systems during low to moderate intensity and long-term exercises. In stressful situations, antioxidant supplements are recommended to prevent ROS damage. We examined the response of SM to ROS generation during exercise using an antioxidant supplement treatment strategy in this study. The findings of this review research are paradoxical due to variances in antioxidant supplements dose and duration, intensity, length, frequency, types of exercise activities, and, in general, the lack of a regular exercise and nutrition strategy. As such, further research in this area is still being felt.

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  • Effect of Genistein Supplementation on Exercise-Induced Inflammation and Oxidative Stress in Mice Liver and Skeletal Muscle

    Cong Wu, Siyi Zhou, Sihui Ma, Katsuhiko Suzuki

    Medicina   57 ( 10 ) 1028 - 1028  2021.09  [Refereed]

     View Summary

    Background and objectives: The purpose of this study was to investigate the influences of oral high-dose genistein (GE) administration on exercise-induced oxidative stress, inflammatory response and tissue damage. Materials and Methods: Thirty-two mice were randomly divided into control group (Con; sedentary/0.5% CMC-Na), GE administrated group (GE; sedentary/GE dosed), exercise group (Ex; exercise/0.5% CMC-Na), or GE administrated plus exercise group (GE + Ex; exercise/GE dosed), mice in the GE and GE + Ex group were given GE orally at the dose of 200 mg/kg weight. Results: Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT) levels, liver interleukin (IL)-6, IL-1β, superoxide dismutase 1 (SOD1), catalase (CAT), hemeoxygenase-1 (HO-1) gene expression levels and skeletal muscle IL-6, nuclear factor erythroid 2-related factor (Nrf2), and HO-1 gene expression levels increased immediately after exhaustive exercise. GE supplementation increased liver protein carbonyl concentrations. On the other hand, GE supplementation significantly decreased SOD1, CAT gene expression levels in the liver and Nrf2, and HO-1 gene expression levels in the skeletal muscles. Conclusions: Acute exercise induced organ damage, inflammation, and oxidative stress in skeletal muscles and the liver. However, a single dose of GE supplementation before exercise did not lead to favorable antioxidant and anti-inflammatory effects in this study.

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  • The Association between Vitamin D and Zinc Status and the Progression of Clinical Symptoms among Outpatients Infected with SARS-CoV-2 and Potentially Non-Infected Participants: A Cross-Sectional Study

    Sahar Golabi, Maryam Adelipour, Sara Mobarak, Maghsud Piri, Maryam Seyedtabib, Reza Bagheri, Katsuhiko Suzuki, Damoon Ashtary-Larky, Fatemeh Maghsoudi, Mahshid Naghashpour

    Nutrients   13 ( 10 ) 3368 - 3368  2021.09  [Refereed]

     View Summary

    Vitamin D and zinc are important components of nutritional immunity. This study compared the serum concentrations of 25-hydroxyvitamin D (25(OH)D) and zinc in COVID-19 outpatients with those of potentially non-infected participants. The association of clinical symptoms with vitamin D and zinc status was also examined. A checklist and laboratory examination were applied to collect data in a cross-sectional study conducted on 53 infected outpatients with COVID-19 and 53 potentially non-infected participants. Serum concentration of 25(OH)D were not significantly lower in patients with moderate illness (19 ± 12 ng/mL) than patients with asymptomatic or mild illness (29 ± 18 ng/mL), with a trend noted for a lower serum concentration of 25(OH)D in moderate than asymptomatic or mild illness patients (p = 0.054). Infected patients (101 ± 18 µg/dL) showed a lower serum concentration of zinc than potentially non-infected participants (114 ± 13 µg/dL) (p = 0.01). Patients with normal (odds ratio (OR), 0.19; p ≤ 0.001) and insufficient (OR, 0.3; p = 0.007) vitamin D status at the second to seventh days of disease had decreased OR of general symptoms compared to patients with vitamin D deficiency. This study revealed the importance of 25(OH)D measurement to predict the progression of general and pulmonary symptoms and showed that infected patients had significantly lower zinc concentrations than potentially non-infected participants.

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  • Elevated Plasma Concentrations of Vitamin D-Binding Protein Are Associated with Lower High-Density Lipoprotein and Higher Fat Mass Index in Overweight and Obese Women

    Leila Setayesh, Abbas Amini, Reza Bagheri, Nariman Moradi, Habib Yarizadeh, Omid Asbaghi, Krista Casazza, Mir Saeed Yekaninejad, Alexei Wong, Katsuhiko Suzuki, Khadijeh Mirzaei

    Nutrients   13 ( 9 ) 3223 - 3223  2021.09  [Refereed]

     View Summary

    (1) Background: Observational studies have established that vitamin D-binding protein (DBP) and 25-hydroxyvitamin D3 (25(OH)D) concentrations are the major factors affecting the bioavailability of 25(OH)D. It has also been shown that poor 25(OH)D bioavailability elevates the risk of obesity and its related cardio-metabolic disorders. However, the relationship between 25(OH)D and DBP concentrations with cardio-metabolic risk factors in overweight and obese cohorts has not been established. Consequently, we evaluated the association between DBP and 25(OH)D concentrations with lipid profile, blood pressure (BP), and body composition in overweight and obese women. (2) Methods: In this cross-sectional study of 236 overweight and obese women, DBP and 25(OH)D concentrations were measured using an enzyme-linked immunosorbent assay. Body composition was assessed via bioelectrical impedance analysis. Lipid profile and BP were assessed by an auto-analyzer and digital BP monitor, respectively. The associations were examined by multivariate logistic regression. (3) Results: The indicated showed an inverse relationship between DBP and high-density lipoprotein (HDL) (p = 0.010) concentrations (where individuals with higher DBP had lower HDL) which, after adjusting for possible cofounders, remained significant (p = 0.006). Moreover, DBP concentration was positively associated with fat mass index (FMI) after adjustment (p = 0.022). No significant relationships were observed among 25(OH)D and target variables. (4) Conclusions: In conclusion, lower concentrations of HDL and higher values of FMI are associated with higher concentrations of DBP in overweight and obese women. These findings present novel awareness regarding the association of DBP with some metabolic and body composition variables in overweight and obese women. However, a two-way causal relationship between DBP and target variables should be considered.

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  • Development and validation of a simple anthropometric equation to predict appendicular skeletal muscle mass

    Ryoko Kawakami, Motohiko Miyachi, Kumpei Tanisawa, Tomoko Ito, Chiyoko Usui, Taishi Midorikawa, Suguru Torii, Kaori Ishii, Katsuhiko Suzuki, Shizuo Sakamoto, Mitsuru Higuchi, Isao Muraoka, Koichiro Oka

    Clinical Nutrition    2021.09  [Refereed]

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  • The Effects of High-Intensity Interval Training vs. Moderate-Intensity Continuous Training on Inflammatory Markers, Body Composition, and Physical Fitness in Overweight/Obese Survivors of Breast Cancer: A Randomized Controlled Clinical Trial

    Babak Hooshmand Moghadam, Fateme Golestani, Reza Bagheri, Neda Cheraghloo, Mozhgan Eskandari, Alexei Wong, Michael Nordvall, Katsuhiko Suzuki, Parisa Pournemati

    Cancers   13 ( 17 ) 4386 - 4386  2021.08  [Refereed]

     View Summary

    Background: Chronic inflammation associated with breast cancer (BC) poses a major challenge in care management and may be ameliorated by physical activity. This randomized controlled trial assessed the effects of a 12-week high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on inflammatory markers, body composition, and physical fitness in BC survivors (BCS). Methods: Forty BCS (age = 57 ± 1 years; body mass [BM] = 74.8 ± 1.5 kg; VO2peak = 20.8 ± 2.1 mL·kg−1·min−1) were randomly assigned to three groups: HIIT (n = 15), MICT (n = 15), or control (CON; n = 15). The intervention groups (HIIT and MICT) performed their respective exercise protocols on a cycle ergometer 3 days/week for 12 weeks while the CON group maintained their current lifestyle. Baseline and post-intervention assessments included body composition (BM, fat mass (FM), lean mass (LM)), physical fitness (VO2peak, lower body strength (LBS), upper body strength (UBS)), and serum concentrations of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), leptin, and adiponectin. Results: Both intervention groups significantly (p < 0.05) decreased BM (HIIT = −1.8 kg, MICT = −0.91 kg), FM (HIIT = −0.81 kg, MICT = −0.18 kg), TNF-α (HIIT = −1.84 pg/mL, MICT = −0.99 pg/mL), IL-6 (HIIT = −0.71 pg/mL, MICT = −0.36 pg/mL), leptin (HIIT = −0.35 pg/mL, MICT = −0.16 pg/mL) and increased VO2peak (HIIT = 0.95 mL·kg−1·min−1, MICT = 0.67 mL·kg−1·min−1), LBS (HIIT = 2.84 kg, MICT = 1.53 kg), UBS (HIIT = 0.53 kg, MICT = 0.53 kg), IL-10 (HIIT = 0.63 pg/mL, MICT = 0.38 pg/mL), and adiponectin (HIIT = 0.23 ng/mL, MICT = 0.1 ng/mL) compared to baseline. The changes in BM, FM, TNF-α, leptin, and LBS were significantly greater in HIIT compared to all other groups. Conclusions: Our findings indicate that compared to the often-recommended MICT, HIIT may be a more beneficial exercise therapy for the improvement of inflammation, body composition and LBS in BCS; and consequently, merits long-term study

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  • The Effect of Saffron Supplementation on Blood Pressure in Adults: A Systematic Review and Dose-Response Meta-Analysis of Randomized Controlled Trials

    Leila Setayesh, Damoon Ashtary-Larky, Cain C. T. Clark, Mahnaz Rezaei Kelishadi, Pardis Khalili, Reza Bagheri, Omid Asbaghi, Katsuhiko Suzuki

    Nutrients   13 ( 8 ) 2736 - 2736  2021.08  [Refereed]

     View Summary

    Background: The favorable influences of saffron supplementation on metabolic diseases have previously been shown. We aimed to assess the effects of saffron supplementation on blood pressure in adults. Methods: A systematic search was performed in Scopus, Embase, and the Cochrane library databases to find randomized controlled trials (RCTs) related to the effect of saffron supplementation on blood pressure in adults up to March 2021. The primary search yielded 182 publications, of which eight RCTs were eligible. Results: Our results showed that saffron supplementation resulted in a significant decrease in systolic blood pressure (weighted mean difference (WMD): −0.65 mmHg; 95% CI: −1.12 to −0.18, p = 0.006) and diastolic blood pressure (DBP) (WMD: −1.23 mmHg; 95% CI: −1.64 to −0.81, p < 0.001). Moreover, saffron supplementation reduced DBP in a non-linear fashion, based on duration (r = −2.45, p-nonlinearity = 0.008). Conclusions: Saffron supplementation may significantly improve both systolic and diastolic blood pressure in adults. It should be noted that the hypotensive effects of saffron supplementation were small and may not reach clinical importance.

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  • Screening of Neutrophil Activating Factors from a Metagenome Library of Sponge-Associated Bacteria

    Yoshiko Okamura, Hirokazu Takahashi, Atsuyuki Shiida, Yuto Hirata, Haruko Takeyama, Katsuhiko Suzuki

    Marine Drugs   19 ( 8 ) 427 - 427  2021.07

     View Summary

    Marine sponge-associated bacteria are known as bio-active compound produce. We have constructed metagenome libraries of the bacteria and developed a metagenomic screening approach. Activity-based screening successfully identified novel genes and novel enzymes; however, the efficiency was only in 1 out of 104 clones. Therefore, in this study, we thought that bioinformatics could help to reduce screening efforts, and combined activity-based screening with database search. Neutrophils play an important role for the immune system to recognize excreted bacterial by-products as chemotactic factors and are recruited to infection sites to kill pathogens via phagocytosis. These excreted by-products are considered critical triggers that engage the immune system to mount a defense against infection, and identifying these factors may guide developments in medicine and diagnostics. We focused on genes encoding amino acid ligase and peptide synthetase and selected from an in-house sponge metagenome database. Cell-free culture medium of each was used in a neutrophil chemiluminescence assay in luminol reaction. The clone showing maximum activity had a genomic sequence expected to produce a molecule like a phospho-N-acetylmuramyl pentapeptide by the metagenome fragment analysis.

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  • Potential Assessment of Dehydration during High-Intensity Training Using a Capacitance Sensor for Oral Mucosal Moisture: Evaluation of Elite Athletes in a Field-Based Survey

    Gen Tanabe, Tetsuya Hasunuma, Yuto Inai, Yasuo Takeuchi, Hiroaki Kobayashi, Kairi Hayashi, Shintaro Shimizu, Nana S Kamiya, Hiroshi Churei, Yuka I Sumita, Katsuhiko Suzuki, Naoki Moriya, Toshiaki Ueno

    Chemosensors   9 ( 8 ) 196 - 196  2021.07  [Refereed]

     View Summary

    Background: The aim of this clinical study was to reveal the relationship between body dehydration and oral mucosa moisture measured by the use of a capacitance sensor for oral epithelial moisture. Methods: The following clinical parameters were recorded from each one of 19 athletes in a one-week period of high-intensity exercise at the U-23 Triathlon Training Camp in summer and winter; body weight, urine specific gravity, oral mucosa moisture, subjective oral thirst, and subjective throat thirst (within 30 min after waking and before breakfast at 7:00 a.m. on Day2 and Day6). Results: There were no significant differences in the mean values of body weight, urine specific gravity, oral mucosa moisture, oral thirst, and throat thirst between Day2 and Day6 in both measurements in summer and winter. The oral mucosa moisture had a moderate negative correlation with urine specific gravity (p < 0.05, r = −0.45). Conclusions: This study suggests that oral mucosal moisture determined using an oral moisture-checking device could be a potential index for assessing dehydration during sports activities.

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  • Wearable Inertial Measurement Unit to Accelerometer-Based Training Monotony and Strain during a Soccer Season: A within-Group Study for Starters and Non-Starters

    Hadi Nobari, Mustafa Sögüt, Rafael Oliveira, Jorge Pérez-Gómez, Katsuhiko Suzuki, Hassane Zouhal

    International Journal of Environmental Research and Public Health   18 ( 15 ) 8007 - 8007  2021.07  [Refereed]

     View Summary

    The purpose of this study was to analyze the intragroup differences in weekly training monotony (TM) and training strain (TS) between starter and non-starter male professional soccer players at accelerometry based variables throughout the periods of a season. TM and TS of different accelerations and decelerations zones for twenty-one players were followed for forty-eight weeks. Regardless of group, players obtained the highest mean TM (starters = 3.3 ± 0.6, non-starters = 2.2 ± 1.1, in arbitrary unit, AU) and TS (starters = 1288.9 ± 265.2, non-starters = 765.4 ± 547.5, AU) scores in the pre-season for accelerations at Zone 1 (<2 m/s2). The results also indicated that both groups exhibited similar TM and TS scores in accelerations at Zones 2 (2 to 4 m/s2) and 3 (>4 m/s2) across the entire season. While the starters showed the highest TM and TS scores at deceleration Zone 1 (<−2 m/s2) in the end-season, the non-starters exhibited the highest scores at the deceleration Zone 1 in pre-season. It seems that in pre-season, coaches applied higher levels of training with greater emphasis on deceleration for non-starters. This tendency was reduced over time for non-starters, while starters presented higher values of deceleration Zone 1. These results highlight the variations in TM and TS across the different periods of a full season according to match starting status among professional soccer players, and the results suggest that non-starter players should receive higher levels of load to compensate for non-participation in matches throughout a soccer season.

    DOI

  • Association between alcohol dietary pattern and prevalence of dyslipidaemia: WASEDA’S Health Study

    Kumpei Tanisawa, Tomoko Ito, Ryoko Kawakami, Chiyoko Usui, Takuji Kawamura, Katsuhiko Suzuki, Shizuo Sakamoto, Kaori Ishii, Isao Muraoka, Koichiro Oka, Mitsuru Higuchi

    British Journal of Nutrition     1 - 31  2021.07  [Refereed]

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    <title>Abstract</title>
    The association between a dietary pattern characterised by high alcohol intake and dyslipidaemia has not been fully investigated. Therefore, the present study aimed to investigate the association between alcohol dietary patterns and the prevalence of dyslipidaemia and its components. This cross-sectional study enrolled 2,171 men and women aged ≥40 years who were alumni of a Japanese university. To identify dietary patterns, a principal component analysis was performed based on the energy-adjusted food intake estimated by a brief-type self-administered diet history questionnaire. Three dietary patterns were identified, the second of which was named the alcohol dietary pattern and was characterised by a high intake of alcoholic beverages, liver, chicken, and fish. This alcohol dietary pattern was associated with reduced low-density lipoprotein cholesterol (LDL-C) levels. The fully adjusted odds ratios (95% confidence intervals) of high LDL-C for the lowest through highest quartile of alcohol dietary pattern score were 1.00 (reference), 0.83 (0.64–1.08), 0.84 (0.64–1.10), and 0.68 (0.49– 0.94), respectively. Subgroup analysis showed that the alcohol dietary pattern was inversely associated with the prevalence of dyslipidaemia in women, whereas it was positively associated with high triglyceride levels in men. In conclusion, the alcohol dietary pattern, characterised by a high intake of alcoholic beverages, liver, chicken, and fish, was associated with the prevalence of dyslipidaemia and its components. This finding provides useful information for the prevention and treatment of dyslipidaemia by modifying the diet.

    DOI

  • Folic Acid Supplementation Improves Glycemic Control for Diabetes Prevention and Management: A Systematic Review and Dose-Response Meta-Analysis of Randomized Controlled Trials

    Omid Asbaghi, Damoon Ashtary-Larky, Reza Bagheri, Seyedeh Parisa Moosavian, Hadi Pourmirzaei Olyaei, Behzad Nazarian, Mahnaz Rezaei Kelishadi, Alexei Wong, Darren G. Candow, Frédéric Dutheil, Katsuhiko Suzuki, Amirmansour Alavi Naeini

    Nutrients   13 ( 7 ) 2355 - 2355  2021.07  [Refereed]

     View Summary

    Background: There is a growing interest in the considerable benefits of dietary supplementations, such as folic acid, on the glycemic profile. We aimed to investigate the effects of folic acid supplementation on glycemic control markers in adults. Methods: Randomized controlled trials examining the effects of folic acid supplementation on glycemic control markers published up to March 2021 were detected by searching online databases, including Scopus, PubMed, Embase, and ISI web of science, using a combination of related keywords. Mean change and standard deviation (SD) of the outcome measures were used to estimate the mean difference between the intervention and control groups at follow-up. Meta-regression and non-linear dose-response analysis were conducted to evaluate the association between pooled effect size and folic acid dosage (mg/day) and duration of the intervention (week). From 1814 detected studies, twenty-four studies reported fasting blood glucose (FBG), fasting insulin, hemoglobin A1C (HbA1C), and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) as an outcome measure. Results: Results revealed significant reductions in FBG (weighted mean difference (WMD): −2.17 mg/dL, 95% CI: −3.69, −0.65, p = 0.005), fasting insulin (WMD: −1.63 pmol/L, 95% CI: −2.53, −0.73, p &lt; 0.001), and HOMA-IR (WMD: −0.40, 95% CI: −0.70, −0.09, p = 0.011) following folic acid supplementation. No significant effect was detected for HbA1C (WMD: −0.27%, 95% CI: −0.73, 0.18, p = 0.246). The dose-response analysis showed that folic acid supplementation significantly changed HOMA-IR (r = −1.30, p-nonlinearity = 0.045) in non-linear fashion. However, meta-regression analysis did not indicate a linear relationship between dose, duration, and absolute changes in FBG, HOMA-IR, and fasting insulin concentrations. Conclusions: Folic acid supplementation significantly reduces some markers of glycemic control in adults. These reductions were small, which may limit clinical applications for adults with type II diabetes. Further research is necessary to confirm our findings.

    DOI

  • Effects of Folic Acid Supplementation on Inflammatory Markers: A Grade-Assessed Systematic Review and Dose–Response Meta-Analysis of Randomized Controlled Trials

    Omid Asbaghi, Damoon Ashtary-Larky, Reza Bagheri, Seyedeh Parisa Moosavian, Behzad Nazarian, Reza Afrisham, Mahnaz Rezaei Kelishadi, Alexei Wong, Frédéric Dutheil, Katsuhiko Suzuki, Amirmansour Alavi Naeini

    Nutrients   13 ( 7 ) 2327 - 2327  2021.07  [Refereed]

     View Summary

    It has been theorized that folic acid supplementation improves inflammation. However, its proven effects on inflammatory markers are unclear as clinical studies on this topic have produced inconsistent results. To bridge this knowledge gap, this systematic review and meta-analysis of randomized controlled trials (RCTs) aimed to evaluate the effects of folic acid supplementation on serum concentrations of the inflammatory markers C-reactive protein (CRP), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α). Methods: To identify eligible RCTs, a systematic search up to April 2021 was completed in PubMed/Medline, Scopus, Web of Science, EMBASE, Cochrane databases, and Google Scholar using relevant keywords. A fix or random-effects model was utilized to estimate the weighted mean difference (WMD) and 95% confidence interval (95% CI). Results: Twelve RCTs were included in the present meta-analysis. The pooled analysis revealed that serum concentrations of CRP (WMD: −0.59 mg/L, 95% CI −0.85 to −0.33, p &lt; 0.001) were significantly reduced following folic acid supplementation compared to placebo, but did not affect serum concentrations of IL-6 (WMD: −0.12, 95% CI −0.95 to 0.72 pg/mL, p = 0.780) or TNF-α (WMD: −0.18, 95% CI −0.86 to 0.49 pg/mL, p = 0.594). The dose–response analysis demonstrated a significant relationship between an elevated dosage of folic acid supplementation and lower CRP concentrations (p = 0.002). Conclusions: We found that folic acid supplementation may improve inflammation by attenuating serum concentrations of CRP but without significant effects on IL-6 and TNF-α. Future RCTs including a larger number of participants and more diverse populations are needed to confirm and expand our findings.

    DOI

  • Acute influence of caffeinated commercially available energy drink on performance, perceived exertion and blood lactate in youth female water polo players

    H Arazi, S Rakhshanfar, E Eghbali, K Suzuki

    Progress in Nutrition   23 ( 2 ) e2021220  2021.07  [Refereed]

    DOI

  • Comparison of the effect of post-exercise cooling with ice slurry ingestion between males and females

    Risa Iwata, Takuji Kawamura, Yuri Hosokawa, Lili Chang, Katsuhiko Suzuki, Isao Muraoka

    Journal of Thermal Biology   99   102979 - 102979  2021.07  [Refereed]

    DOI

  • Regular exercise stimulates endothelium autophagy via IL‐1 signaling in ApoE deficient mice

    Mitsuharu Okutsu, Mami Yamada, Ken Tokizawa, Shuri Marui, Katsuhiko Suzuki, Vitor A. Lira, Kei Nagashima

    The FASEB Journal   35 ( 7 )  2021.07  [Refereed]

    DOI

  • The Effects of Nano-Curcumin Supplementation on Risk Factors for Cardiovascular Disease: A GRADE-Assessed Systematic Review and Meta-Analysis of Clinical Trials

    Damoon Ashtary-Larky, Mahnaz Rezaei Kelishadi, Reza Bagheri, Seyedeh Parisa Moosavian, Alexei Wong, Sayed Hossein Davoodi, Pardis Khalili, Frédéric Dutheil, Katsuhiko Suzuki, Omid Asbaghi

    Antioxidants   10 ( 7 ) 1015 - 1015  2021.06  [Refereed]

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    Previous studies have indicated that curcumin supplementation may be beneficial for cardiometabolic health; however, current evidence regarding the effects of its nanorange formulations, popularly known as “nano-curcumin”, remains unclear. This systematic review and meta-analysis aimed to determine the impact of nano-curcumin supplementation on risk factors for cardiovascular disease. PubMed, Scopus, Embase, and ISI web of science were systematically searched up to May 2021 using relevant keywords. All randomized controlled trials (RCTs) investigating the effects of nano-curcumin supplementation on cardiovascular disease risk factors were included. Meta-analysis was performed using random-effects models, and subgroup analysis was performed to explore variations by dose and baseline risk profiles. According to the results of this study, nano-curcumin supplementation was associated with improvements in the glycemic profile by decreasing fasting blood glucose (FBG) (WMD: −18.14 mg/dL; 95% CI: −29.31 to −6.97; p = 0.001), insulin (WMD: −1.21 mg/dL; 95% CI: −1.43 to −1.00; p &lt; 0.001), and HOMA-IR (WMD: −0.28 mg/dL; 95% CI: −0.33 to −0.23; p &lt; 0.001). Interestingly, nano-curcumin supplementation resulted in increases in high-density lipoprotein (HDL) (WMD: 5.77 mg/dL; 95% CI: 2.90 to 8.64; p &lt; 0.001). In terms of other lipid profile markers (triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL)), subgroup analyses showed that nano-curcumin supplementation had more favorable effects on lipid profiles in individuals with dyslipidemia at baseline. Nano-curcumin supplementation also showed favorable anti-inflammatory effects by decreasing C-reactive protein (CRP) (WMD: −1.29 mg/L; 95% CI: −2.15 to −0.44; p = 0.003) and interleukin-6 (IL-6) (WMD: −2.78 mg/dL; 95% CI: −3.76 to −1.79; p &lt; 0.001). Moreover, our results showed the hypotensive effect of nano-curcumin, evidenced by a decrease in systolic blood pressure (SBP). In conclusion, our meta-analysis suggests that nano-curcumin supplementation may decline cardiovascular disease risk by improving glycemic and lipid profiles, inflammation, and SBP. Future large-scale investigations with longer durations are needed to expand on our findings.

    DOI

  • Potential Improvement in Rehabilitation Quality of 2019 Novel Coronavirus by Isometric Training System; Is There “Muscle-Lung Cross-Talk”?

    Hadi Nobari, Mohamad Fashi, Arezoo Eskandari, Jorge Pérez-Gómez, Katsuhiko Suzuki

    International Journal of Environmental Research and Public Health   18 ( 12 ) 6304 - 6304  2021.06  [Refereed]

     View Summary

    The novel Coronavirus Disease 2019 (COVID-19) crisis is now present in more than 200 countries. It started in December 2019 and has, so far, led to more than 149, 470,968 cases, 3,152,121 deaths, and 127,133,013 survivors recovered by 28 April 2021. COVID-19 has a high morbidity, and mortality of 2%, on average, whereas most people are treated after a period of time. Some people who recover from COVID-19 are left with 20 to 30% decreased lung function. In this context, exercise focused on skeletal muscle with minimal lung involvement could potentially play an important role. Regular exercise protects against diseases associated with chronic low-grade systemic inflammation. This long-term effect of exercise may be ascribed to the anti-inflammatory response elicited by an acute bout of exercise, which is partly mediated by muscle-derived myokines. The isometric training system seems to have this feature, because this system is involved with the skeletal muscle as the target tissue. However, no studies have examined the effect of exercise on the treatment and recovery of COVID-19, and, more importantly, “muscle–lung cross-talk” as a mechanism for COVID-19 treatment. It is suggested that this theoretical construct be examined by researchers.

    DOI

  • Determinants of Resting Oxidative Stress in Middle-Aged and Elderly Men and Women: WASEDA’S Health Study

    Takuji Kawamura, Kumpei Tanisawa, Ryoko Kawakami, Chiyoko Usui, Tomoko Ito, Hiroki Tabata, Nobuhiro Nakamura, Sayaka Kurosawa, Wonjun Choi, Sihui Ma, Zsolt Radak, Susumu S. Sawada, Katsuhiko Suzuki, Kaori Ishii, Shizuo Sakamoto, Koichiro Oka, Mitsuru Higuchi, Isao Muraoka

    Oxidative Medicine and Cellular Longevity   2021   1 - 11  2021.06  [Refereed]

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    Previous studies have not investigated the determinants of resting oxidative stress, including physical fitness, as it relates to redox regulation. The present study therefore was aimed at identifying lifestyle and biological factors that determine resting oxidative stress, including objectively measured physical fitness. In 873 middle-aged and elderly men and women, age and anthropometric parameters, lifestyle-related parameters, medication and supplementation status, physical fitness, biochemical parameters, and nutritional intake status, as well as three plasma oxidative stress markers: protein carbonyl (PC), F2-isoprostane (F2-IsoP), and thiobarbituric acid reactive substances (TBARS), were surveyed and measured. The determinants of PC, F2-IsoP, and TBARS in all participants were investigated using stepwise multiple regression analysis. In PC, age (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M1">
    <mi>β</mi>
    <mo>=</mo>
    <mo>−</mo>
    <mn>0.11</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M2">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.002</mn>
    </math>
    </inline-formula>), leg extension power (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M3">
    <mi>β</mi>
    <mo>=</mo>
    <mo>−</mo>
    <mn>0.12</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M4">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.008</mn>
    </math>
    </inline-formula>), BMI (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M5">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.12</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M6">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.004</mn>
    </math>
    </inline-formula>), and HDL-C (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M7">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.08</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M8">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.040</mn>
    </math>
    </inline-formula>) were included in the regression model (adjusted <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M9">
    <msup>
    <mrow>
    <mi>R</mi>
    </mrow>
    <mrow>
    <mn>2</mn>
    </mrow>
    </msup>
    <mo>=</mo>
    <mn>0.018</mn>
    </math>
    </inline-formula>). In the F2-IsoP, smoking status (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M10">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.07</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M11">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.060</mn>
    </math>
    </inline-formula>), BMI (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M12">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.07</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M13">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.054</mn>
    </math>
    </inline-formula>), and HbA1c (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M14">
    <mi>β</mi>
    <mo>=</mo>
    <mo>−</mo>
    <mn>0.06</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M15">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.089</mn>
    </math>
    </inline-formula>) were included in the regression model (adjusted <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M16">
    <msup>
    <mrow>
    <mi>R</mi>
    </mrow>
    <mrow>
    <mn>2</mn>
    </mrow>
    </msup>
    <mo>=</mo>
    <mn>0.006</mn>
    </math>
    </inline-formula>). In TBARS, glucose (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M17">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.18</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M18">
    <mi>P</mi>
    <mo>&lt;</mo>
    <mn>0.001</mn>
    </math>
    </inline-formula>), CRF (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M19">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.16</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M20">
    <mi>P</mi>
    <mo>&lt;</mo>
    <mn>0.001</mn>
    </math>
    </inline-formula>), age (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M21">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.15</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M22">
    <mi>P</mi>
    <mo>&lt;</mo>
    <mn>0.001</mn>
    </math>
    </inline-formula>), TG (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M23">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.11</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M24">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.001</mn>
    </math>
    </inline-formula>), antioxidant supplementation (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M25">
    <mi>β</mi>
    <mo>=</mo>
    <mn>0.10</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M26">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.002</mn>
    </math>
    </inline-formula>), and HbA1c (<inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M27">
    <mi>β</mi>
    <mo>=</mo>
    <mo>−</mo>
    <mn>0.13</mn>
    </math>
    </inline-formula>, <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M28">
    <mi>P</mi>
    <mo>=</mo>
    <mn>0.004</mn>
    </math>
    </inline-formula>) were included in the regression model (adjusted <inline-formula>
    <math xmlns="http://www.w3.org/1998/Math/MathML" id="M29">
    <msup>
    <mrow>
    <mi>R</mi>
    </mrow>
    <mrow>
    <mn>2</mn>
    </mrow>
    </msup>
    <mo>=</mo>
    <mn>0.071</mn>
    </math>
    </inline-formula>). In conclusion, the present study showed that age, anthropometric index, lifestyle-related parameters, medication and supplementation status, objectively measured physical fitness, biochemical parameters, and nutritional intake status explain less than 10% of oxidative stress at rest.

    DOI

  • Exercise in an Overweight Patient with Covid-19: A Case Study

    Amir Hossein Ahmadi Hekmatikar, Mahdieh Molanouri Shamsi, Zahra Sadat Zabhi Ashkazari, Katsuhiko Suzuki

    International Journal of Environmental Research and Public Health   18 ( 11 ) 5882 - 5882  2021.05  [Refereed]

     View Summary

    Coronavirus (COVID-19) is a dangerous infectious disease that is easily transmitted and which is called an acute respiratory syndrome. With the spread of the coronavirus around the world and its epidemic among humans, we are losing many humans. The long process of treatment in hospitalized patients who are receiving intensive care and medication is associated with physical weakness. It has been suggested that lifelong exercise can create a safe margin for a person that allows them to avoid becoming infected with the virus. The current study was conducted to assess the effects of low-intensity exercise and breathing exercises on cardiorespiratory responses and physical status in an overweight 20-year-old woman infected with COVID-19. The patient was referred to Hazrat Ali Ibn Abitaleb Hospital in Rafsanjan. The patient had initial symptoms of coronavirus including weakness, shortness of breath, fever, and chills, and the initial tests confirmed that the person was infected with the coronavirus. Although COVID-19 reduces respiration and blood oxygen and severely reduces movement and physical activity, low-intensity rehabilitation and breathing exercises along with medication can improve blood oxygen status, resting heart rate, blood pressure, and hand power status in patients and possibly speeding up the healing process. The results of the present study show that low-intensity exercise and breathing exercises in patients with COVID-19, whose disease severity is mild to moderate, can be performed safely under the supervision of their physicians to prevent the disease process.

    DOI

  • Effects of Folic Acid Supplementation on Oxidative Stress Markers: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

    Omid Asbaghi, Matin Ghanavati, Damoon Ashtary-Larky, Reza Bagheri, Mahnaz Rezaei Kelishadi, Behzad Nazarian, Michael Nordvall, Alexei Wong, Frédéric Dutheil, Katsuhiko Suzuki, Amirmansour Alavi Naeini

    Antioxidants   10 ( 6 ) 871 - 871  2021.05  [Refereed]

     View Summary

    (1) Background: This systematic review and meta-analysis aimed to assess the effects of folic acid supplementation on oxidative stress markers. (2) Methods: Online database including PubMed, Scopus, Web of Science, and Cochrane were searched up to January 2021, to retrieve randomized controlled trials (RCTs) which examined the effect of folic acid supplementation on markers of oxidative stress. Meta-analyses were carried out using a random-effects model. I2 index was used to evaluate the heterogeneity of RCTs. (3) Results: Among the initial 2322 studies that were identified from electronic databases search, 13 studies involving 1013 participants were eligible. Pooled effect size from 13 studies indicated that folic acid supplementation elicits a significant rise in serum concentrations of glutathione (GSH) (WMD: 219.01 umol/L, 95% CI 59.30 to 378.71, p = 0.007) and total antioxidant capacity (TAC) (WMD: 91.70 umol/L, 95% CI 40.52 to 142.88, p &lt; 0.001) but has no effect on serum concentrations of nitric oxide (NO) (WMD: 2.61 umol/L, 95% CI −3.48 to 8.72, p = 0.400). In addition, folic acid supplementation significantly reduced serum concentrations of malondialdehyde (MDA) (WMD: −0.13 umol/L, 95% CI −0.24 to −0.02, p = 0.020). (4) Conclusions: This meta-analysis study suggests that folic acid supplementation may significantly improve markers within the antioxidative defense system by increasing serum concentrations of GSH and TAC and decreasing serum concentrations of MDA.

    DOI

  • The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males

    Takaki Tominaga, Tsukasa Ikemura, Koichi Yada, Kazue Kanda, Kaoru Sugama, Sihui Ma, Wonjun Choi, Mayu Araya, Jiapeng Huang, Nobuhiro Nakamura, Katsuhiko Suzuki

    Antioxidants   10 ( 6 ) 866 - 866  2021.05  [Refereed]

     View Summary

    Strenuous exercise induces organ damage, inflammation and oxidative stress. To prevent exercise-induced organ damage, inflammation and oxidative stress, rehydrating may be an effective strategy. In the present study, we aimed to examine whether beverage intake after exhaustive exercise to recover from dehydration prevents such disorders. Thirteen male volunteers performed incremental cycling exercise until exhaustion. Immediately after exercise, the subjects drank an electrolyte containing water (rehydrate trial: REH) or did not drink any beverage (control trial: CON). Blood samples were collected before (Pre), immediately (Post), 1 h and 2 h after exercise. Urine samples were also collected before (Pre) and 2 h after exercise. We measured biomarkers of organ damage, inflammation and oxidative stress in blood and urine. Biomarkers of muscle, renal and intestinal damage and inflammation increased in the blood and urine after exercise. However, changes in biomarkers of organ damage and inflammation did not differ between trials (p &gt; 0.05). The biomarker of oxidative stress, thiobarbituric acid reactive substances (TBARS), in plasma, showed different changes between trials (p = 0.027). One hour after exercise, plasma TBARS concentration in REH had a higher trend than that in CON (p = 0.052), but there were no significant differences between Pre and the other time points in each trial. These results suggest that beverage intake after exercise does not attenuate exercise-induced organ damage, inflammation or oxidative stress in healthy males. However, rehydration restores exercise-induced oxidative stress more quickly.

    DOI

  • A Low-Protein High-Fat Diet Leads to Loss of Body Weight and White Adipose Tissue Weight via Enhancing Energy Expenditure in Mice

    Yifeng Rang, Sihui Ma, Jiao Yang, Huan Liu, Katsuhiko Suzuki, Chunhong Liu

    Metabolites   11 ( 5 ) 301 - 301  2021.05  [Refereed]

     View Summary

    Obesity has become a worldwide health problem over the past three decades. During obesity, metabolic dysfunction of white adipose tissue (WAT) is a key factor increasing the risk of type 2 diabetes. A variety of diet approaches have been proposed for the prevention and treatment of obesity. The low-protein high-fat diet (LPHF) is a special kind of high-fat diet, characterized by the intake of a low amount of protein, while compared to typical high-fat diet, may induce weight loss and browning of WAT. Physical activity is another effective intervention to treat obesity by reducing WAT mass, inducing browning of WAT. In order to determine whether an LPHF, along with exercise enhanced body weight loss and body fat loss as well as the synergistic effect of an LPHF and exercise on energy expenditure in a mice model, we combined a 10-week LPHF with an 8-week forced treadmill training. Meanwhile, a traditional high-fat diet (HPHF) containing the same fat and relatively more protein was introduced as a comparison. In the current study, we further analyzed energy metabolism-related gene expression, plasma biomarkers, and related physiological changes. When comparing to HPHF, which induced a dramatic increase in body weight and WAT weight, the LPHF led to considerable loss of body weight and WAT, without muscle mass and strength decline, while it exhibited a risk of liver and pancreas damage. The mechanism underlying the LPHF-induced loss of body weight and WAT may be attributed to the synergistically upregulated expression of Ucp1 in WAT and Fgf21 in the liver, which may enhance energy expenditure. The 8-week training did not further enhance weight loss and increased plasma biomarkers of muscle damage when combined with LPHF. Furthermore, LPHF reduced the expression of fatty acid oxidation-related genes in adipose tissues, muscle tissues, and liver. Our results indicated that an LPHF has potential for obesity treatment, while the physiological condition should be monitored during application.

    DOI

  • A Brief Overview of Oxidative Stress in Adipose Tissue with a Therapeutic Approach to Taking Antioxidant Supplements

    Shima Taherkhani, Katsuhiko Suzuki, Ruheea Taskin Ruhee

    Antioxidants   10 ( 4 ) 594 - 594  2021.04  [Refereed]

     View Summary

    One of the leading causes of obesity associated with oxidative stress (OS) is excessive consumption of nutrients, especially fast-foods, and a sedentary lifestyle, characterized by the ample accumulation of lipid in adipose tissue (AT). When the body needs energy, the lipid is broken down into glycerol (G) and free fatty acids (FFA) during the lipolysis process and transferred to various tissues in the body. Materials secreted from AT, especially adipocytokines (interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α)) and reactive oxygen species (ROS), are impressive in causing inflammation and OS of AT. There are several ways to improve obesity, but researchers have highly regarded the use of antioxidant supplements due to their neutralizing properties in removing ROS. In this review, we have examined the AT response to OS to antioxidant supplements focusing on animal studies. The results are inconsistent due to differences in the study duration and diversity in animals (strain, age, and sex). Therefore, there is a need for different studies, especially in humans.

    DOI

  • Does regular resistance exercise improve resting and intradialytic pain and haemodynamic measures in sedentary chronic haemodialysis women?

    H. Arazi, M. Poursardar, B. Taati, K. Suzuki

    Comparative Exercise Physiology   17 ( 3 ) 235 - 241  2021.04  [Refereed]

     View Summary

    Pain is one of the major problems in haemodialysis patients. Regular resistance exercise (RE), as an effective lifestyle modification, may play a role in reducing pain through changes in blood pressure (BP). Therefore, we aimed to evaluate the effect of RE training on pain, BP, and heart rate. In a randomised controlled trial, eighteen sedentary women completed the study in RE (n=10) or control (n=8) groups. In the exercise group, the patients had a circuit RE program in non-dialysis days, two times a week, for six weeks. The RE protocol consisted of six exercises performing with 10 repetitions at 50-60% of 1-repetition maximum. Resting and intradialytic pain threshold (algometer using a 1 kg pressure load), BP, and heart rate were measured 48 h before and after the intervention. There were no significant differences from pre- to post-intervention, or between the groups regarding pain threshold, systolic and diastolic BP, and heart rate at rest and during the haemodialysis process (P&gt;0.05). Although the 6-week low-intensity RE training in non-dialysis days was safe for chronic haemodialysis women, it could not change resting and intradialytic pain and hemodynamic measures.

    DOI

  • Endurance Training Regulates Expression of Some Angiogenesis-Related Genes in Cardiac Tissue of Experimentally Induced Diabetic Rats

    Mojdeh Khajehlandi, Lotfali Bolboli, Marefat Siahkuhian, Mohammad Rami, Mohammadreza Tabandeh, Kayvan Khoramipour, Katsuhiko Suzuki

    Biomolecules   11 ( 4 ) 498 - 498  2021.03  [Refereed]

     View Summary

    Exercise can ameliorate cardiovascular dysfunctions in the diabetes condition, but its precise molecular mechanisms have not been entirely understood. The aim of the present study was to determine the impact of endurance training on expression of angiogenesis-related genes in cardiac tissue of diabetic rats. Thirty adults male Wistar rats were randomly divided into three groups (N = 10) including diabetic training (DT), sedentary diabetes (SD), and sedentary healthy (SH), in which diabetes was induced by a single dose of streptozotocin (50 mg/kg). Endurance training (ET) with moderate-intensity was performed on a motorized treadmill for six weeks. Training duration and treadmill speed were increased during five weeks, but they were kept constant at the final week, and slope was zero at all stages. Real-time polymerase chain reaction (RT-PCR) analysis was used to measure the expression of myocyte enhancer factor-2C (MEF2C), histone deacetylase-4 (HDAC4) and Calmodulin-dependent protein kinase II (CaMKII) in cardiac tissues of the rats. Our results demonstrated that six weeks of ET increased gene expression of MEF2C significantly (p &lt; 0.05), and caused a significant reduction in HDAC4 and CaMKII gene expression in the DT rats compared to the SD rats (p &lt; 0.05). We concluded that moderate-intensity ET could play a critical role in ameliorating cardiovascular dysfunction in a diabetes condition by regulating the expression of some angiogenesis-related genes in cardiac tissues.

    DOI

  • Icing after eccentric contraction-induced muscle damage perturbs the disappearance of necrotic muscle fibers and phenotypic dynamics of macrophages in mice

    Masato Kawashima, Noriaki Kawanishi, Takaki Tominaga, Katsuhiko Suzuki, Anna Miyazaki, Itsuki Nagata, Makoto Miyoshi, Motoi Miyakawa, Tohma Sakuraya, Takahiro Sonomura, Takamitsu Arakawa

    Journal of Applied Physiology   130 ( 5 ) 1410 - 1420  2021.03  [Refereed]

     View Summary

    Icing is still one of the most common treatments to acute skeletal muscle damage in sports medicine. However, previous studies using rodents reported the detrimental effect of icing on muscle regeneration following injury. This study aimed to elucidate the critical factors governing the impairment of muscle regeneration by icing with a murine model of eccentric contraction-induced muscle damage by electrical stimulation. Because of icing after muscle injury, the infiltration of polynuclear and mononuclear cells into necrotic muscle fibers was retarded and attenuated, leading to the persistent presence of necrotic cellular debris. These phenomena coincided with the delayed emergence and sustained accumulation of Pax7+ myogenic cells within the regenerating area. Additionally, due to icing, delayed and/or sustained infiltration of M1 macrophages was noted in accordance with the perturbed expression patterns of inflammation-related factors, including tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10). The key myogenic regulatory factors (i.e., MyoD and myogenin) involved in the activation/proliferation and differentiation of myogenic precursor cells were not altered by icing during the regenerative process. A detailed analysis of regenerating myofibers by size distribution at day 14 after muscle damage showed that the ratio of small regenerating fibers to total regenerating fibers was higher in icing-treated animals than in untreated animals. These findings suggest that icing following muscle damage blunts the efficiency of muscle regeneration by perturbing the removal of necrotic myofibers and phenotypic dynamics of macrophages rather than affecting myogenic factors.

    DOI

  • Creatine Supplementation, Physical Exercise and Oxidative Stress Markers: A Review of the Mechanisms and Effectiveness

    Hamid Arazi, Ehsan Eghbali, Katsuhiko Suzuki

    Nutrients   13 ( 3 ) 869 - 869  2021.03  [Refereed]

     View Summary

    Oxidative stress is the result of an imbalance between the generation of reactive oxygen species (ROS) and their elimination by antioxidant mechanisms. ROS degrade biogenic substances such as deoxyribonucleic acid, lipids, and proteins, which in turn may lead to oxidative tissue damage. One of the physiological conditions currently associated with enhanced oxidative stress is exercise. Although a period of intense training may cause oxidative damage to muscle fibers, regular exercise helps increase the cells’ ability to reduce the ROS over-accumulation. Regular moderate-intensity exercise has been shown to increase antioxidant defense. Endogenous antioxidants cannot completely prevent oxidative damage under the physiological and pathological conditions (intense exercise and exercise at altitude). These conditions may disturb the endogenous antioxidant balance and increase oxidative stress. In this case, the use of antioxidant supplements such as creatine can have positive effects on the antioxidant system. Creatine is made up of two essential amino acids, arginine and methionine, and one non-essential amino acid, glycine. The exact action mechanism of creatine as an antioxidant is not known. However, it has been shown to increase the activity of antioxidant enzymes and the capability to eliminate ROS and reactive nitrogen species (RNS). It seems that the antioxidant effects of creatine may be due to various mechanisms such as its indirect (i.e., increased or normalized cell energy status) and direct (i.e., maintaining mitochondrial integrity) mechanisms. Creatine supplement consumption may have a synergistic effect with training, but the intensity and duration of training can play an important role in the antioxidant activity. In this study, the researchers attempted to review the literature on the effects of creatine supplementation and physical exercise on oxidative stress.

    DOI

  • Anti-Inflammatory and Antioxidant Effects of Dietary Supplementation and Lifestyle Factors

    Llion Arwyn Roberts, Katsuhiko Suzuki

    Antioxidants   10 ( 3 ) 371 - 371  2021.03  [Refereed]

     View Summary

    Trends relating to specific diets and lifestyle factors like physical (in) activity have formed in recent times [...]

    DOI

  • HMB Supplementation and Resistance Training: Current Overview on Inflammation, Oxidative Stress and Cardiovascular Risk Factors

    Hamid Arazi, Behzad Taati, Katsuhiko Suzuki

    Recent Research Advances in Biology Vol. 5     155 - 168  2021.02  [Refereed]

    DOI

  • A Low-Carbohydrate Ketogenic Diet and Treadmill Training Enhanced Fatty Acid Oxidation Capacity but Did Not Enhance Maximal Exercise Capacity in Mice

    Sihui Ma, Jiao Yang, Takaki Tominaga, Chunhong Liu, Katsuhiko Suzuki

    Nutrients   13 ( 2 ) 611 - 611  2021.02  [Refereed]

     View Summary

    The low-carbohydrate ketogenic diet (LCKD) is a dietary approach characterized by the intake of high amounts of fat, a balanced amount of protein, and low carbohydrates, which is insufficient for metabolic demands. Previous studies have shown that an LCKD alone may contribute to fatty acid oxidation capacity, along with endurance. In the present study, we combined a 10-week LCKD with an 8-week forced treadmill running program to determine whether training in conjunction with LCKD enhanced fatty acid oxidation capacity, as well as whether the maximal exercise capacity would be affected by an LCKD or training in a mice model. We found that the lipid pool and fatty acid oxidation capacity were both enhanced following the 10-week LCKD. Further, key fatty acid oxidation related genes were upregulated. In contrast, the 8-week training regimen had no effect on fatty acid and ketone body oxidation. Key genes involved in carbohydrate utilization were downregulated in the LCKD groups. However, the improved fatty acid oxidation capacity did not translate into an enhanced maximal exercise capacity. In summary, while favoring the fatty acid oxidation system, an LCKD, alone or combined with training, had no beneficial effects in our intensive exercise-evaluation model. Therefore, an LCKD may be promising to improve endurance in low- to moderate-intensity exercise, and may not be an optimal choice for those partaking in high-intensity exercise.

    DOI

  • The Effect of Acute Intense Exercise on Activity of Antioxidant Enzymes in Smokers and Non-Smokers

    Hadi Nobari, Hamzeh Abdi Nejad, Mehdi Kargarfard, Soghra Mohseni, Katsuhiko Suzuki, José Carmelo Adsuar, Jorge Pérez-Gómez

    Biomolecules   11 ( 2 ) 171 - 171  2021.01  [Refereed]

     View Summary

    Acute intense exercise causes significant oxidative stress and consequently an increase in total antioxidant capacity; however, the mechanisms and combined effects of intense exercise and smoking on oxidative stress among active and non-active smokers are not clear. The aim of this study was to investigate the effect of acute intense exercise on antioxidant enzyme activity responses in active and non-active individuals exposed to cigarette smoke. The study included 40 subjects who were equally classified as: smokers that did exercise (SE), smokers that did not do exercise (SnE), non-smokers that did exercise (NSE), and non-smokers that did not do exercise (NSnE). The adjusted Astrand test was used to exhaust the subjects. Salivary enzymes of peroxidase (POX), catalase (CAT), and superoxide dismutase (SOD) were measured, by spectrophotometry methods, at 3 different time points: pre-test (TP1), post-test (TP2), and one hour after finishing the test (TP3). Significant (p &lt; 0.05) group x time interactions were found for the three enzymes. Salivary POX, CAT and SOD increased in all groups from TP1 to TP2 and decreased from TP2 to TP3. Only the NSE showed a significant difference between TP1 to TP3 in POX and SOD by +0.011 ± 0.007 and +0.075 ± 0.020 (U/mL), respectively. The NSE showed significantly higher activity of POX, CAT and SOD in TP2 compared to the other groups. Furthermore, NSE and NSnE had higher activity of POX, CAT and SOD in TP1 and TP3 (p &lt; 0.05) compared with SE and SnE. Only in the NSnE, were no differences observed in CAT compared with SE and SnE in TP3. These results showed that the antioxidant activity at rest and in the recovery time after the acute intense exercise was lower in SE and SnE compared with NSE and NSnE, suggesting that smoking habit may reduce the ameliorating effect of regular physical activity on acute exercise-induced oxidative stress.

    DOI

  • Ramadan Fasting During the COVID-19 Pandemic; Observance of Health, Nutrition and Exercise Criteria for Improving the Immune System

    Majid Taati Moghadam, Behzad Taati, Seyed Mojtaba Paydar Ardakani, Katsuhiko Suzuki

    Frontiers in Nutrition   7   349  2021.01  [Refereed]

     View Summary

    Fasting is one of the religious rituals of Muslims worldwide who refrain from eating foods and liquids every year during Ramadan. This year (2020), Ramadan is very different from previous years due to the outbreak of a terrible microscopic giant called coronavirus disease 2019 (COVID-19). The pandemic COVID-19 has made Ramadan very important this year because the virus has infected millions of people around the world and killed thousands, especially people with immunodeficiency. In dealing with COVID-19, maintaining good hygiene and supporting the immune system are effective, preventive approaches. Moderate exercise training and proper nutrition are the most important factors to support immune function. Lack of facilities, poor health and many traditions that lead to public community gatherings have made many Islamic countries susceptible to this dangerous virus. In such an unprecedented situation, there are many Muslims who doubt whether they can fast or not. Therefore, the proposal of usable exercise programs and effective nutritional strategies is imperative. In this study, we will look at the proposed health effects of fasting and its impact on the immune system, the effects of Ramadan intermittent fasting on resting values and responses of immunological/antioxidant biomarkers in elite and recreational athletes, together with the important health, nutrition, and exercise advice that fasting people need to follow in the event of a COVID-19 outbreak.

    DOI

  • Effect of Exercise Intensity on Cell-Mediated Immunity

    Katsuhiko Suzuki, Harumi Hayashida

    Sports   9 ( 1 ) 8 - 8  2021.01  [Refereed]

     View Summary

    Moderate-intensity exercise is considered to enhance immune function and to be useful for preventing acute upper respiratory infections and similar conditions. Many people practice low-intensity short-duration exercise with the expectation of a beneficial effect on immunocompetency. However, it is difficult to affirm the existence of definite evidence of such a benefit. In this article, we discuss the effects of low-intensity short-duration exercise on cell-mediated immunity, and contrast them to the effects of high-intensity and long-duration exercise. Whereas high-intensity exercise induces inflammation and reduces cell-mediated immune system function, low-intensity exercise does not appear to have a large effect on either inflammation or cell-mediated immune function. Low-intensity exercises such as walking and yoga, which are helpful to relieve stress, cannot be considered as harmful to the immune system. Although yoga was shown to impose fewer restrictions on breathing and physical strain, the evidence that yoga enhances cell-mediated immunity remains insufficient. Therefore, further studies are needed to examine the exercise mode that may be most effective for improvement of immune functions.

    DOI

  • Changes in Urinary Biomarkers of Organ Damage, Inflammation, Oxidative Stress, and Bone Turnover Following a 3000-m Time Trial

    Takaki Tominaga, Sihui Ma, Kaoru Sugama, Kazue Kanda, Chiaki Omae, Wonjun Choi, Shunsuke Hashimoto, Katsuhiko Aoyama, Yasunobu Yoshikai, Katsuhiko Suzuki

    Antioxidants   10 ( 1 ) 79 - 79  2021.01  [Refereed]

     View Summary

    Strenuous exercise induces organ damage, inflammation, and oxidative stress. Currently, to monitor or investigate physiological conditions, blood biomarkers are frequently used. However, blood sampling is perceived to be an invasive method and may induce stress. Therefore, it is necessary to establish a non-invasive assessment method that reflects physiological conditions. In the present study, we aimed to search for useful biomarkers of organ damage, inflammation, oxidative stress, and bone turnover in urine following exercise. Ten male runners participated in this study and performed a 3000-m time trial. We measured biomarkers in urine collected before and immediately after exercise. Renal damage markers such as urea protein, albumin, N-acetyl-β-D-glucosaminidase (NAG), and liver-fatty acid binding protein (L-FABP), and an intestinal damage marker, intestine-fatty acid binding protein (I-FABP), increased following exercise (p &lt; 0.05). However, a muscle damage marker, titin N-terminal fragments, did not change (p &gt; 0.05). Inflammation-related factors (IRFs), such as interleukin (IL)-1β, IL-1 receptor antagonist (IL-1ra), IL-6, complement (C) 5a, myeloperoxidase (MPO), calprotectin, monocyte chemoattractant protein (MCP)-1, and macrophage colony-stimulating factor (M-CSF), increased whereas IRFs such as IL-4 and IL-10 decreased following exercise (p &lt; 0.05). IRFs such as tumor necrosis factor (TNF)-α, IL-2, IL-8, IL-12p40, and interferon (IFN)-γ did not change (p &gt; 0.05). Oxidative stress markers, such as thiobarbituric acid reactive substances (TBARS) and nitrotyrosine, did not change following exercise (p &gt; 0.05) whereas 8-hydroxy-2′-deoxyguanosine (8-OHdG) decreased (p &lt; 0.05). Bone resorption markers, such as cross-linked N-telopeptide of type I collagen (NTX) and deoxypyridinoline (DPD), did not change following exercise (p &gt; 0.05). These results suggest that organ damage markers and IRFs in urine have the potential to act as non-invasive indicators to evaluate the effects of exercise on organ functions.

    DOI

  • Physical activity and nutrition guidelines to help with the fight against COVID-19

    Kayvan Khoramipour, Aref Basereh, Amirhoseein Ahmadi Hekmatikar, Lindy Castell, Ruheea Taskin Ruhee, Katsuhiko Suzuki

    Journal of Sports Sciences   39 ( 1 ) 101 - 107  2021.01  [Refereed]

    DOI

  • An acute eccentric exercise increases circulating myomesin 3 fragments

    Minjung Lee, Jaehoon Shin, Tatsuya Kato, Kazue Kanda, Satoshi Oikawa, Jun Sakuma, Kaoru Sugama, Yasuo Kawakami, Katsuhiko Suzuki, Takayuki Akimoto

    The Journal of Physiological Sciences   71 ( 1 ) 1 - 6  2021.01  [Refereed]

     View Summary

    <title>Abstract</title>Discovery of blood biomarkers to evaluate exercise-induced muscle damage have attracted many researchers and coaches. This study aimed to determine changes in circulating myomesin 3 fragments as a novel biomarker for exercise-induced muscle damage. Nine healthy males performed 10 sets of 40 repetitions of one-leg calf-raise exercise by the load corresponding to the half of their body weight. Muscle symptoms were evaluated by a visual analog scale (VAS). Blood samples were collected before and 2, 4, 24, 48, 72, and 96 h post-exercise. Plasma myomesin 3 fragments levels were significantly increased at 96 h after the eccentric exercise. The myomesin 3 fragments levels were correlated with other biomarkers of muscle damage and the muscle symptoms. These results suggest that the circulating myomesin 3 fragments levels are potential biomarkers reflecting eccentric exercise-induced muscle damage.

    DOI

  • 運動と免疫・炎症研究のスポーツ栄養への応用可能性:最近の知見

    鈴木克彦

    日本スポーツ栄養研究誌   14   3 - 9  2021.01  [Refereed]

    Authorship:Lead author

  • Metallomics Analysis for Assessment of Toxic Metal Burdens in Infants/Children and Their Mothers: Early Assessment and Intervention Are Essential

    Hiroshi Yasuda, Toyoharu Tsutsui, Katsuhiko Suzuki

    Biomolecules   11 ( 1 ) 6 - 6  2020.12  [Refereed]

     View Summary

    Accumulation of toxic metals in infants/children is of serious concern worldwide, from the viewpoint of their harmful effects on the normal growth and development. This metallomics study investigates the extent of toxic metal burdens in infants/children and the relationship to those in their mothers for 77 child/mother pair subjects. For mercury, its geometric mean concentration in infants/children was of similar level to that in their mothers, and a high-significant close correlation was observed between infants/children and their mothers (β = 0.758, r = 0.539, p &lt; 0.0001). A significant but less intimate mother/child relationship was observed for arsenic (β = 0.301, r = 0.433), lead (β = 0.444, r = 0.471) and aluminum (β = 0.379, r = 0.451). Remarkably, the burden levels of lead, cadmium and aluminum in infants/children were approximately three times higher than those in their mothers (p &lt; 0.0001), and the burden levels in some individuals were several tens of times higher than in the mothers. In contrast, some essential metal levels such as zinc, magnesium and calcium in infants/children were significantly lower than those in their mothers, and 29 individuals (37.7%) in the child subjects were estimated to be zinc-deficient. In addition, significant inverse correlations were observed between zinc and lead (r = −0.267, p = 0.019), and magnesium and arsenic (r = −0.514, p &lt; 0.0001). These findings suggest that these toxic metal burdens and essential metal deficiencies in infants/children are of serious concern for their neurodevelopment, indicating that the early assessment and intervention are crucial. It is expected that larger epidemiological and intervention studies will provide a reasonable and essential pathway for intervention of neurodevelopment disorders.

    DOI

  • microRNAs slow translating ribosomes to prevent protein misfolding

    Hiroaki Sako, Takayuki Akimoto, Katsuhiko Suzuki, Takashi Ushida, Tadashi Yamamoto

       2020.12  [Refereed]

     View Summary

    <title>Abstract</title>An evolutionarily conserved mechanism, use of non-optimal codons, slows ribosomes during translation to allow proper folding of nascent polypeptides. However, until now, it was unknown whether any eukaryote-specific mechanisms exist for this purpose. Here, we propose that miRNAs slow translating ribosomes to prevent protein misfolding, with little negative effect on protein abundance. To prove this, we bioinformatically analyze ribosome profiling and miRNA binding sites and biochemically confirm that miRNA deficiency causes severe misfolding, which is rescued by slowing translating ribosomes. We demonstrate that non-cleaving shRNAs, targeting regions where elongation rates become faster in miRNA-deficient cells, improve protein folding with minimal effects on protein abundance. These results reveal broader functionality of miRNAs and a previously unknown mechanism to prevent protein misfolding.

    <sec><title>One Sentence Summary</title>Eukaryote use of miRNAs prevents protein misfolding in a target-specific manner.

    </sec>

    DOI

  • Differences between sexes in thermoregulatory responses and exercise time during endurance exercise in a hot environment following pre-cooling with ice slurry ingestion

    Risa Iwata, Takuji Kawamura, Yuri Hosokawa, Lili Chang, Katsuhiko Suzuki, Isao Muraoka

    Journal of Thermal Biology   94   102746 - 102746  2020.12  [Refereed]

     View Summary

    This study aimed to examine differences between sexes in thermoregulatory responses and exercise time after ice slurry ingestion in a hot environment. Twenty-four healthy adults (male n = 12, body weight (BW) = 65.8 ± 10.3; female n = 12, BW = 58.2 ± 10.0) ingested 7.5 g/kg of either ice slurry at −1 °C (ICE) or control water at 20 °C (CON) before cycling at 55%VO max in a hot environment (controlled at 38 °C, 40% relative humidity). Rectal (Tre) and skin (Tsk) temperature, heart rate, sweat rate, respiratory gases, ratings of thermal sensation (TS), thermal comfort (TC), and rating of perceived exertion (RPE) were measured. Ice slurry did not improve exercise time in both sexes despite Tre was significantly lower in ICE than CON in both sexes. Tre, Tsk, HR, sweat rate and TS did not differ between sexes. TC and RPE in ICE were significantly higher during exercise in males than in females. In conclusion, there were no sex differences in the effects of pre-cooling with ice slurry ingestion; however, pre-cooling with ice slurry may be more effective in mitigating ratings of TC and RPE in females than males. 2

    DOI PubMed

  • Visceral fat and cardiorespiratory fitness with prevalence of pre-diabetes/diabetes mellitus among middle-aged and elderly Japanese people: WASEDA’S Health Study

    Chiyoko Usui, Ryoko Kawakami, Kumpei Tanisawa, Tomoko Ito, Hiroki Tabata, Satoshi Iizuka, Takuji Kawamura, Taishi Midorikawa, Susumu S. Sawada, Suguru Torii, Shizuo Sakamoto, Katsuhiko Suzuki, Kaori Ishii, Koichiro Oka, Isao Muraoka, Mitsuru Higuchi

    PLOS ONE   15 ( 10 ) e0241018 - e0241018  2020.10  [Refereed]  [International journal]

     View Summary

    The relationships between cardiorespiratory fitness (CRF) measurements not confounded by adiposity and the prevalence of pre-diabetes mellitus (pre-DM) and diabetes mellitus (DM) are not well known. Thus, we aimed to investigate the associations of visceral fat (VF) and CRF with the prevalence of pre-DM/DM among Japanese adults. The study included 970 individuals (327 women and 643 men) who were 40-87 years old and had complete health examinations, abdominal fat area, and fitness data from WASEDA'S Health Study during 2015-2018. The VF area was measured using magnetic resonance imaging. CRF was measured using a cycle ergometer and was defined as VO2peak divided by fat free mass. The pre-DM/DM was identified based on the questionnaire and fasting blood tests. The odds ratios (ORs) and 95% confidence intervals (CIs) for prevalence of pre-DM/DM were calculated. Seventy-three participants had pre-DM and 48 participants had DM. Compared to the low VF group, the high VF group had a higher prevalence of pre-DM/DM (OR: 1.87, 95% CI: 1.18-2.96), although no significant relationship was observed between CRF and pre-DM/DM prevalence (P for trend = 0.239). The sub-group analyses also revealed no significant relationship between CRF and pre-DM/DM prevalence in the low VF group (P for trend = 0.979), although CRF values were inversely related to the prevalence of pre-DM/DM in the high VF group (P for trend = 0.024). Although CRF was not independently related to the prevalence of pre-DM/DM after adjusting for adiposity, higher VF values were related to a higher prevalence of pre-DM/DM. In addition, CRF levels were inversely associated with the prevalence of pre-DM/DM only among high VF individuals.

    DOI PubMed

  • Exercise, redox system and neurodegenerative diseases

    Helong Quan, Erika Koltai, Katsuhiko Suzuki, Aderbal S. Aguiar, Ricardo Pinho, Istvan Boldogh, Istvan Berkes, Zsolt Radak

    Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease   1866 ( 10 ) 165778 - 165778  2020.10  [Refereed]

    DOI

  • Cut‐offs for calf circumference as a screening tool for low muscle mass: WASEDA'S Health Study

    Ryoko Kawakami, Motohiko Miyachi, Susumu S. Sawada, Suguru Torii, Taishi Midorikawa, Kumpei Tanisawa, Tomoko Ito, Chiyoko Usui, Kaori Ishii, Katsuhiko Suzuki, Shizuo Sakamoto, Mitsuru Higuchi, Isao Muraoka, Koichiro Oka

    Geriatrics & Gerontology International   20 ( 10 ) 943 - 950  2020.10  [Refereed]

    DOI

  • A Short Overview of Changes in Inflammatory Cytokines and Oxidative Stress in Response to Physical Activity and Antioxidant Supplementation

    Shima Taherkhani, Katsuhiko Suzuki, Lindy Castell

    Antioxidants   9 ( 9 ) 886 - 886  2020.09  [Refereed]

     View Summary

    Excessive release of inflammatory cytokines and oxidative stress (OS) are triggering factors in the onset of chronic diseases. One of the factors that can ensure health in humans is regular physical activity. This type of activity can enhance immune function and dramatically prevent the spread of the cytokine response and OS. However, if physical activity is done intensely at irregular intervals, it is not only unhealthy but can also lead to muscle damage, OS, and inflammation. In this review, the response of cytokines and OS to exercise is described. In addition, it is focused predominantly on the role of reactive oxygen and nitrogen species (RONS) generated from muscle metabolism and damage during exercise and on the modulatory effects of antioxidant supplements. Furthermore, the influence of factors such as age, sex, and type of exercise protocol (volume, duration, and intensity of training) is analyzed. The effect of antioxidant supplements on improving OS and inflammatory cytokines is somewhat ambiguous. More research is needed to understand this issue, considering in greater detail factors such as level of training, health status, age, sex, disease, and type of exercise protocol.

    DOI

  • 全身持久力と下腿周の組合せと糖尿病有病率の関係 WASEDA'S Health Study

    王 棟, 澤田 亨, 佐藤 孝太郎, 郡山 さくら, 田端 宏樹, 谷澤 薫平, 川上 諒子, 石井 香織, 鈴木 克彦, 樋口 満, 岡 浩一朗, 坂本 静男

    日本臨床運動療法学会学術集会プログラム・抄録集   39回   75 - 75  2020.09

  • 最大歩行速度と生活習慣病有病率の関係 横断研究 WASEDA'S Health Study

    佐藤 孝太朗, 澤田 亨, 王 棟, 郡山 さくら, 田端 宏樹, 谷澤 薫平, 川上 諒子, 伊藤 智子, 薄井 澄誉子, 石井 香織, 鈴木 克彦, 樋口 満, 岡 浩一朗, 坂本 静男

    日本臨床運動療法学会学術集会プログラム・抄録集   39回   83 - 83  2020.09

  • Oxidative Stress and Inflammation Induced by Waterpipe Tobacco Smoking Despite Possible Protective Effects of Exercise Training: A Review of the Literature

    Behzad Taati, Hamid Arazi, Katsuhiko Suzuki

    Antioxidants   9 ( 9 ) 777 - 777  2020.08  [Refereed]

     View Summary

    The prevalence of waterpipe tobacco smoking (WTS), which is also known as ghalyan, shisha or hookah, is increasing rapidly around the world, especially among youth. Growing interest in this form of tobacco smoking can be traced, in part, to the use of flavored tobacco products, social acceptability as a safer option than cigarettes, and its consideration as a relaxation method or entertainment. However, there is a well-established association between WTS and oxidative stress that causes irreversible chronic pathological conditions such as cardiovascular and respiratory problems, as well as different types of cancers, and thus increases the risk of mortality. Clearly, induction of inflammation status through increased reactive oxygen species (ROS), which in turn leads to oxidative stress and harm to lipids, DNA, and proteins, is the most plausible mechanism to explain the potential harmful effects of WTS. Unlike WTS, well-designed exercise training programs increase ROS to the extent that it is beneficial to the body. In this study, we aimed to review available evidence on the impact of exercise training on oxidative stress and inflammation status. We also summarize the effect of acute and chronic WTS on different exercise capacities.

    DOI

  • Effects of Upper and Lower Limb Plyometric Training Program on Components of Physical Performance in Young Female Handball Players

    Mehrez Hammami, Nawel Gaamouri, Katsuhiko Suzuki, Roy J. Shephard, Mohamed Souhaiel Chelly

    Frontiers in Physiology   11   1028  2020.08  [Refereed]

    DOI

  • Effects of Ramadan Intermittent Fasting on Gut Hormones and Body Composition in Males with Obesity

    Hassane Zouhal, Reza Bagheri, Raoua Triki, Ayoub Saeidi, Alexei Wong, Anthony C. Hackney, Ismail Laher, Katsuhiko Suzuki, Abderraouf Ben Abderrahman

    International Journal of Environmental Research and Public Health   17 ( 15 ) 5600 - 5600  2020.08  [Refereed]

     View Summary

    We studied the effects of Ramadan intermittent fasting (RIF) on gut hormones (leptin, glucagon-like peptide-1 (GLP-1), peptide YY (PYY), cholecystokinin (CCK), and ghrelin) in males with obesity. Thirty sedentary males were randomly allocated to either an experimental group (EG, n = 15) or a control group (CG, n = 15). The EG group completed their Ramadan fasting rituals (30 days), whereas the CG continued with their normal daily habits. Blood samples were collected at four time points: 24 h before the start of Ramadan (T0), on the 15th day of Ramadan (T1), the day after the end of Ramadan (T2) and 21 days after Ramadan (T3). There were significant pre-to-post improvements for leptin (p = 0.01, d = 1.52), GLP-1 (p = 0.022, d = 0.75), PYY (p = 0.031, d = 0.69) and CCK (p = 0.027, d = 0.81) in the EG, with no interaction effect for ghrelin (p = 0.74; d = 0.008). No significant changes (p &gt; 0.05) occurred in plasma volume variations (ΔPV) after RIF in both EG (−0.03 ± 0.01%) and CG (0.06 ± 0.07%). RIF represents an effective strategy to modify appetite-regulating hormones, leading to improved body composition indices and reduced obesity.

    DOI

  • The systemic role of SIRT1 in exercise mediated adaptation

    Zsolt Radak, Katsuhiko Suzuki, Aniko Posa, Zita Petrovszky, Erika Koltai, Istvan Boldogh

    Redox Biology   35   101467 - 101467  2020.08  [Refereed]

    DOI

  • Acute Responses of Cardiac Biomarkers to Intermittent and Continuous Exercise Are Related to Age Difference but Not I/D Polymorphism in the ACE Gene

    Akram Falahati, Hamid Arazi, Katsuhiko Suzuki

    Frontiers in Physiology   11  2020.07  [Refereed]

    DOI

  • Influence of different preceding exercises on fat metabolism during subsequent endurance exercise

    Ryosuke Fujii, Nobuhiro Nakamura, Takuji Kawamura, Tsukasa Ikemura, Masayuki Konishi, Katsuhiko Suzuki, Shizuo Sakamoto, Isao Muraoka

    Gazzetta Medica Italiana Archivio per le Scienze Mediche   179 ( 6 ) 393 - 403  2020.07  [Refereed]

    DOI

  • The Integrative Role of Sulforaphane in Preventing Inflammation, Oxidative Stress and Fatigue: A Review of a Potential Protective Phytochemical

    Ruheea Taskin Ruhee, Katsuhiko Suzuki

    Antioxidants   9 ( 6 ) 521 - 521  2020.06  [Refereed]

     View Summary

    Cruciferous vegetables hold a myriad of bioactive molecules that are renowned for possessing unique medicinal benefits. Sulforaphane (SFN) is one of the potential nutraceuticals contained within cruciferous vegetables that is useful for improving health and diseased conditions. The objective of this review is to discuss the mechanistic role for SFN in preventing oxidative stress, fatigue, and inflammation. Direct and indirect research evidence is reported to identify the nontoxic dose of SFN for human trials, and effectiveness of SFN to attenuate inflammation and/or oxidative stress. SFN treatment modulates redox balance via activating redox regulator nuclear factor E2 factor-related factor (Nrf2). SFN may play a crucial role in altering the Keap1/Nrf2/ARE pathway (an intricate response to many stimuli or stress), which induces Nrf2 target gene activation to reduce oxidative stress. In addition, SFN reduces inflammation by suppressing centrally involved inflammatory regulator nuclear factor-kappa B (NF-κB), which in turn downregulates the expression of proinflammatory cytokines and mediators. Exercise may induce a significant range of fatigue, inflammation, oxidative stress, and/or organ damage due to producing excessive reactive oxygen species (ROS) and inflammatory cytokines. SFN may play an effective role in preventing such damage via inducing phase 2 enzymes, activating the Nrf2/ARE signaling pathway or suppressing nuclear translocation of NF-κB. In this review, we summarize the integrative role of SFN in preventing fatigue, inflammation, and oxidative stress, and briefly introduce the history of cruciferous vegetables and the bioavailability and pharmacokinetics of SFN reported in previous research. To date, very limited research has been conducted on SFN’s effectiveness in improving exercise endurance or performance. Therefore, more research needs to be carried out to determine the effectiveness of SFN in the field of exercise and lifestyle factors.

    DOI

  • Organosulfur Compounds: A Review of Their Anti-inflammatory Effects in Human Health

    Ruheea Taskin Ruhee, Llion Arwyn Roberts, Sihui Ma, Katsuhiko Suzuki

    Frontiers in Nutrition   7   64  2020.06  [Refereed]

    DOI

  • Characterization and Modulation of Systemic Inflammatory Response to Exhaustive Exercise in Relation to Oxidative Stress

    Katsuhiko Suzuki, Takaki Tominaga, Ruheea Taskin Ruhee, Sihui Ma

    Antioxidants   9 ( 5 ) 401 - 401  2020.05  [Refereed]

     View Summary

    Exhaustive exercise induces systemic inflammatory responses, which are associated with exercise-induced tissue/organ damage, but the sources and triggers are not fully understood. Herein, the basics of inflammatory mediator cytokines and research findings on the effects of exercise on systemic inflammation are introduced. Subsequently, the association between inflammatory responses and tissue damage is examined in exercised and overloaded skeletal muscle and other internal organs. Furthermore, an overview of the interactions between oxidative stress and inflammatory mediator cytokines is provided. Particularly, the transcriptional regulation of redox signaling and pro-inflammatory cytokines is described, as the activation of the master regulatory factor nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is involved directly or indirectly in controlling pro-inflammatory genes and antioxidant enzymes expression, whilst nuclear factor-kappa B (NF-κB) regulates the pro-inflammatory gene expression. Additionally, preventive countermeasures against the pathogenesis along with the possibility of interventions such as direct and indirect antioxidants and anti-inflammatory agents are described. The aim of this review is to give an overview of studies on the systematic inflammatory responses to exercise, including our own group as well as others. Moreover, the challenges and future directions in understanding the role of exercise and functional foods in relation to inflammation and oxidative stress are discussed.

    DOI

  • Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study

    Sihui Ma, Takaki Tominaga, Kazue Kanda, Kaoru Sugama, Chiaki Omae, Shunsuke Hashimoto, Katsuhiko Aoyama, Yasunobu Yoshikai, Katsuhiko Suzuki

    Biomedicines   8 ( 3 ) 51 - 51  2020.03  [Refereed]

     View Summary

    Prolonged strenuous exercise may induce inflammation, cause changes in gastrointestinal permeability, and lead to other unfavorable biological changes and diseases. Nutritional approaches have been used to prevent exercise-induced inflammatory responses and gastrointestinal disorders. Hyperimmunized milk, obtained by immunizing cows against specific antigens, promotes the development of immunity against pathogens, promotes anti-inflammatory effects, and protects intestinal function. Immune protein (IMP) is a concentrated product of hyperimmunized milk and is a more promising means of supplementation to protect against acute infections and inflammation. To determine whether IMP has protective properties against exercise-induced gastrointestinal dysfunction and inflammation, we examined biochemical markers, intestinal damage markers, and pro-/anti-inflammatory profiles of young male runners using a randomized, placebo controlled, cross-over design. Urine samples were collected and used for measurements of creatinine, N-acetyl-β-d-glucosaminidase, osmotic pressure, and specific gravity. Titin was measured as a muscle damage marker. Further, urine concentrations of complement 5a, calprotectin, fractalkine, myeloperoxidase, macrophage colony-stimulating factor, monocyte chemotactic protein-1, intestinal fatty acid binding protein (I-FABP), interferon (IFN)-γ, interleukin (IL)-1β, IL-1 receptor antagonist, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p40, and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assays. We demonstrated that urine osmotic pressure, urine specific gravity, I-FABP, IFN-γ, IL-1β, and TNF-α were reduced by 8 weeks of IMP supplementation, indicating that IMP may have potential in preventing strenuous exercise-induced renal dysfunction, increased intestinal permeability, and inflammation. Thus, IMP supplementation may be a feasible nutritional approach for the prevention of unfavorable exercise-induced symptoms.

    DOI

  • Changes in Urinary Titin N-terminal Fragment Concentration after Concentric and Eccentric Exercise

    Shota Yamaguchi, Katsuhiko Suzuki, Takayuki Inami, Kazue Kanda, Zhao Hanye, Junichi Okada

    Journal of Sports Science & Medicine   19 ( 1 ) 121 - 129  2020.03  [Refereed]

  • Protective Effects of Sulforaphane on Exercise-Induced Organ Damage via Inducing Antioxidant Defense Responses

    Ruheea Taskin Ruhee, Sihui Ma, Katsuhiko Suzuki

    Antioxidants   9 ( 2 ) 136 - 136  2020.02  [Refereed]

     View Summary

    Regular exercise is beneficial to maintain a healthy lifestyle, but the beneficial effects are lost in the case of acute exhaustive exercise; this causes significant inflammation, oxidative stress along with organ damage. Recently, sulforaphane (SFN), an indirect antioxidant, has drawn special attention for its potential protective effect against inflammation and oxidative stress. However, no studies have been performed regarding acute exhaustive exercise-induced organ damage in association with SFN administration. Therefore, the aim of this study was to investigate the effects of SFN on acute exhaustive exercise-induced organ damage and the mechanisms involved. To perform the study, we divided mice into four groups: Control, SFN, exercise, and SFN plus exercise. The SFN group was administered orally (50 mg/kg body wt) 2 h before the running test. We measured plasma levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), and acute exhaustive exercise significantly increased these biomarkers. In addition, the mRNA expression of pro-inflammatory cytokines, IL-6, IL-1β, and TNF-α, were significantly increased in the liver of exercise group. However, the SFN plus exercise group showed a significant reduction in the expression of cytokines and blood biomarkers of tissue damage or cell death. Furthermore, we measured mRNA expression of Nrf2, heme oxygenase (HO)-1, and antioxidant defense enzymes expression, i.e., superoxide dismutase (SOD1), catalase (CAT), and glutathione peroxidase (GPx1) in the liver. The expression of all these biomarkers was significantly upregulated in the SFN plus exercise group. Collectively, SFN may protect the liver from exhaustive exercise-induced inflammation via inducing antioxidant defense response through the activation of Nrf2/HO-1 signal transduction pathway.

    DOI

  • The effects of water temperature on gastric motility and energy intake in healthy young men

    Kyoko Fujihira, Yuka Hamada, Takuma Yanaoka, Ryo Yamamoto, Katsuhiko Suzuki, Masashi Miyashita

    European Journal of Nutrition   59 ( 1 ) 103 - 109  2020.02  [Refereed]

    DOI

  • N-terminal fragments of titin in urine as a biomarker for eccentric exercise-induced muscle damage

    Shota Yamaguchi, Katsuhiko Suzuki, Kazue Kanda, Junichi Okada

    The Journal of Physical Fitness and Sports Medicine   9 ( 1 ) 21 - 29  2020.01  [Refereed]

    DOI

  • The effects of pre-meal drink volume on gastric motility and energy intake in healthy men

    Kyoko Fujihira, Yuka Hamada, Katsuhiko Suzuki, Masashi Miyashita

    Physiology & Behavior   213   112726 - 112726  2020.01  [Refereed]

    DOI

  • Effect of Acacia Polyphenol Supplementation on Exercise-Induced Oxidative Stress in Mice Liver and Skeletal Muscle

    Koichi Yada, Llion Arwyn Roberts, Natsumi Oginome, Katsuhiko Suzuki

    Antioxidants   9 ( 1 ) 29 - 29  2019.12  [Refereed]

     View Summary

    The purpose of this study was to investigate the effects of acacia polyphenol (AP) supplementation on exercise-induced oxidative stress in mouse liver and skeletal muscle. Plasma aspartate aminotransferase (AST), liver and skeletal muscle levels of thiobarbituric acid reactive substance (TBARS), and levels of skeletal muscle protein carbonyls increased immediately after exhaustive exercise. Exhaustive exercise also decreased liver glutathione (GSH). These results suggest that the exhaustive exercise used in this study induced tissue damage and oxidative stress. Contrary to our expectations, AP supplementation increased plasma AST and alanine aminotransferase activities, liver levels of TBARS, and protein carbonyls. Furthermore, AP supplementation decreased glutathione and glutathione peroxidase activity in the liver. On the other hand, AP supplementation decreased TBARS levels in skeletal muscle. These results suggest that oral high-dose AP administration decreased oxidative stress in skeletal muscle but induced oxidative stress in the liver and increased hepatotoxicity.

    DOI

  • Negative Mood Is Associated with Diet and Dietary Antioxidants in University Students During the Menstrual Cycle: A Cross-Sectional Study from Guangzhou, China

    Lingling Bu, Yuting Lai, Yingyan Deng, Chenlu Xiong, Fengying Li, Li Li, Katsuhiko Suzuki, Sihui Ma, Chunhong Liu

    Antioxidants   9 ( 1 ) 23 - 23  2019.12  [Refereed]

     View Summary

    Postpubescent females may have negative mood or premenstrual syndrome during the menstrual cycle; with the emotional and physical symptoms interfering with their quality of life. Little is known about the relationship of dietary behaviors and dietary antioxidant intake with negative mood or premenstrual syndrome in university students in China; so we explored the relationship between negative mood and dietary behavior in female university students during the three menstrual cycle phases. Random sampling was used to enroll 88 individuals from a university in Guangzhou; China in the study. Data were collected using self-administered questionnaires. Descriptive statistics and multiple logistic regression analyses were performed. During the menstrual phase, tea, black coffee and carbonated beverage intake was higher in the group with a high negative affect scale score than in the low score group (p &lt; 0.05). Likewise; during the premenstrual phase, fresh fruit (banana and red Chinese dates) intake was higher in the group with a high negative affect scale score than in the low-score group (p &lt; 0.05). The logistic regression analysis results showed that negative mood was positively associated with tea, coffee, and carbonated beverage intake during the menstrual phase (β = 0.21, p = 0.0453, odds ratio = 1.23), and negative mood was positively associated with banana and red Chinese dates intake during the premenstrual phase (β = 0.59, p = 0.0172, odds ratio = 1.81). Our results suggest that negative mood may be associated with diet and specific food in university postpubescent females.

    DOI

  • Micronutrient Intake Adequacy in Men and Women with a Healthy Japanese Dietary Pattern.

    Tomoko Ito, Kumpei Tanisawa, Ryoko Kawakami, Chiyoko Usui, Kaori Ishii, Katsuhiko Suzuki, Shizuo Sakamoto, Isao Muraoka, Koichiro Oka, Mitsuru Higuchi

    Nutrients   12 ( 1 )  2019.12  [Refereed]  [International journal]

     View Summary

    This study examined the relationship between a healthy Japanese dietary pattern and micronutrient intake adequacy based on the Dietary Reference Intakes for Japanese 2015 (DRIs-J 2015) in men and women. A cross-sectional study was conducted in 1418 men and 795 women aged 40-87 years, who participated in the Waseda Alumni's Sports, Exercise, Daily Activity, Sedentariness, and Health Study. Dietary patterns were derived from principal component analysis of the consumption of 52 food and beverage items, which were assessed by a validated brief-type self-administered diet history questionnaire. Micronutrient intakes were quantified using the dietary reference intakes score (DRIs-score) for 21 micronutrients (based on DRIs-J 2015). The healthy dietary pattern score was significantly and positively correlated with the intakes of all 21 micronutrients used for constructing the DRIs-score in men and in women (each, p < 0.001). In both sexes, the healthy dietary pattern scores were strongly and positively associated with DRIs-scores (in men: ρ = 0.806, p < 0.001; in women: ρ = 0.868, p < 0.001), and the DRIs-scores reached a plateau around the highest tertile of the healthy dietary pattern score. These results indicate that a healthy Japanese dietary pattern is associated with adequate micronutrient intakes based on the DRIs-J 2015 in both men and women.

    DOI PubMed

  • Loss of microRNA-23–27–24 clusters in skeletal muscle is not influential in skeletal muscle development and exercise-induced muscle adaptation

    Minjung Lee, Shogo Wada, Satoshi Oikawa, Katsuhiko Suzuki, Takashi Ushida, Takayuki Akimoto

    Scientific Reports   9 ( 1 ) 1092  2019.12  [Refereed]

     View Summary

    MicroRNAs are small regulatory noncoding RNAs that repress gene expression at the posttranscriptional level. Previous studies have reported that the expression of miR-23, miR-27, and miR-24, driven from two miR-23-27-24 clusters, is altered by various pathophysiological conditions. However, their functions in skeletal muscle have not been clarified. To define the roles of the miR-23-27-24 clusters in skeletal muscle, we generated double-knockout (dKO) mice muscle-specifically lacking the miR-23-27-24 clusters. The dKO mice were viable and showed normal growth. The contractile and metabolic features of the muscles, represented by the expression of the myosin heavy chain and the oxidative markers PGC1-alpha and COX IV, were not altered in the dKO mice compared with wild-type mice. The dKO mice showed increased cross-sectional areas of the oxidative fibers. However, this dKO did not induce functional changes in the muscles. The dKO mice also showed normal adaptation to voluntary wheel running for 4 weeks, including the glycolytic-to-oxidative fiber type switch, and increases in mitochondrial markers, succinate dehydrogenase activity, and angiogenesis. In conclusion, our data demonstrate that the miR-23-27-24 clusters have subtle effects on skeletal muscle development and endurance-exercise-induced muscle adaptation.

    DOI PubMed

  • Acute Effects of Resistance Exercise and the Use of GH or IGF-1 Hormones on Oxidative Stress and Antioxidant Markers in Bodybuilders

    Heidar Mohammadjafari, Hamid Arazi, Nematollah Nemati, Tahereh Bagherpoor, Katsuhiko Suzuki

    Antioxidants   8 ( 12 ) 587 - 587  2019.11  [Refereed]

     View Summary

    The aim of this study was to examine the influence of peptide hormone use on oxidative stress and antioxidant responses to a single session of resistance exercise in male bodybuilders. Forty-five male bodybuilders were divided into three groups: bodybuilders using growth hormone for at least 1 year (i.e., 3 to 4 times in the year) (GH-user, n = 15), bodybuilders using insulin-like growth factor-1 for at least 1 year (i.e., 3 to 4 times in the year) (IGF-1-user, n = 15), and peptide hormone-free bodybuilders (Non-user, n = 15). The heavy resistance exercise protocol consisted of five sets with 80% of one repetition maximum for six exercises. Blood samples were obtained pre and post heavy resistance exercise (HRE) in order to evaluate changes in oxidative stress (8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO)) and antioxidant biomarkers (glutathione peroxidase (GPx), catalase (CAT) and glutamate (GLU)) level. All the experimental groups showed increases in MDA (p = 0.038), NO (p = 0.028), GPx (p = 0.012), and GLU (p = 0.003) concentrations after resistance exercise. For 8-OHdG, the Non-user and IGF-1-user groups indicated increases at post-exercise (p = 0.001), without any significant changes in the GH-user group (p = 0.87). In addition, the changes in serum GPx and GLU levels were greater for the GH-user group than the Non-user and IGF-1-user groups (p = 0.001). In conclusion, HRE induced significant increases in 8-OHdG (except to GH-user group), MDA, NO, GPx, and GLU levels with greater changes in GPx and GLU for the GH-user group.

    DOI

  • Sulforaphane Protects Cells against Lipopolysaccharide-Stimulated Inflammation in Murine Macrophages

    Ruheea Taskin Ruhee, Sihui Ma, Katsuhiko Suzuki

    Antioxidants   8 ( 12 ) 577 - 577  2019.11  [Refereed]

     View Summary

    Inflammation is an essential part for the general or innate immune defenses to defend against tissue damage and accelerate the curing process by providing protection against pathogens. Sulforaphane (SFN) is a natural isothiocyanate that has potential properties against inflammation, along with other protective functions. The purpose of this study was to examine the mechanism of its protective effect on lipopolysaccharide (LPS)-induced inflammation in Raw 264.7 macrophages. Here, we compared LPS-challenged macrophages with or without SFN pretreatment. Macrophages were pre-incubated for 6 h with a wide range of concentrations of SFN (0 to 50 µM), and then treated with LPS for 24 h. Nitric oxide (NO) concentration and gene expression of different inflammatory mediators, i.e., interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β, were measured. SFN neither directly reacted with cytokines, nor with NO. To understand the mechanisms, we performed analyses of the expression of regulatory enzyme inducible nitic oxide synthase (iNOS), the transcription factor NF-E2-related factor 2 (Nrf2), and its enzyme heme-oxygenase (HO)-1. Our results revealed that LPS increased significantly the expression of inflammatory cytokines and concentration of NO in non-treated cells. SFN was able to prevent the expression of NO and cytokines through regulating inflammatory enzyme iNOS and activation of Nrf2/HO-1 signal transduction pathway.

    DOI

  • 中高年者における最大脂質酸化量の関連要因(Related factors of maximal fat oxidation in middle-aged and older people)

    Tabata Hiroki, Tanisawa Kumpei, Konishi Masayuki, Ito Tomoko, Kawakami Ryoko, Usui Chiyoko, Sawada Susumu, Ishii Kaori, Midorikawa Taishi, Torii Suguru, Suzuki Katsuhiko, Oka Koichiro, Higuchi Mitsuru, Sakamoto Shizuo

    The Journal of Physical Fitness and Sports Medicine   8 ( 6 ) 317 - 317  2019.11

  • Resistance exercise on two consecutive days induces cortisol, CK, IgA responses in active young males

    Hamid Arazi, Ehsan Eghbali, Katsuhiko Suzuki, Meysam Mahdavi

    Apunts. Medicina de l'Esport   54 ( 204 ) 131 - 138  2019.10  [Refereed]

    DOI

  • Muscle-derived SDF-1α/CXCL12 modulates endothelial cell proliferation but not exercise training-induced angiogenesis.

    Yamada M, Hokazono C, Tokizawa K, Marui S, Iwata M, Lira VA, Suzuki K, Miura S, Nagashima K, Okutsu M

    American journal of physiology. Regulatory, integrative and comparative physiology   317 ( 6 ) R770-R779  2019.10  [Refereed]  [International journal]

     View Summary

    Chemokines are critical mediators of angiogenesis in several physiological and pathological conditions; however, a potential role for muscle-derived chemokines in exercise-stimulated angiogenesis in skeletal muscle remains poorly understood. Here, we postulated that the chemokine stromal cell-derived factor-1 (SDF-1α/C-X-C motif chemokine ligand 12: CXCL12), shown to promote neovascularization in several organs, contributes to angiogenesis in skeletal muscle. We found that CXCL12 is abundantly expressed in capillary-rich oxidative soleus and exercise-trained plantaris muscles. CXCL12 mRNA and protein were also abundantly expressed in muscle-specific peroxisome proliferator-activated receptor γ coactivator 1α transgenic mice, which have a high proportion of oxidative muscle fibers and capillaries when compared with wild-type littermates. We then generated CXCL12 muscle-specific knockout mice but observed normal baseline capillary density and normal angiogenesis in these mice when they were exercise trained. To get further insight into a potential CXCL12 role in a myofiber-endothelial cell crosstalk, we first mechanically stretched C2C12 myotubes, a model known to induce stretch-related chemokine release, and observed increased CXCL12 mRNA and protein. Human umbilical vein endothelial cells (HUVECs) exposed to conditioned medium from cyclically stretched C2C12 myotubes displayed increased proliferation, which was dependent on CXCL12-mediated signaling through the CXCR4 receptor. However, HUVEC migration and tube formation were unaltered under these conditions. Collectively, our findings indicate that increased muscle contractile activity enhances CXCL12 production and release from muscle, potentially contributing to endothelial cell proliferation. However, redundant signals from other angiogenic factors are likely sufficient to sustain normal endothelial cell migration and tube formation activity, thereby preserving baseline capillary density and exercise training-mediated angiogenesis in muscles lacking CXCL12.

    DOI PubMed

  • Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes

    Naoyoshi Sakitani, Takahiro Maekawa, Kumiko Saitou, Katsuhiko Suzuki, Shuhei Murase, Masakuni Tokunaga, Daisuke Yoshino, Keisuke Sawada, Atsushi Takashima, Motoshi Nagao, Toru Ogata, Yasuhiro Sawada

    Journal of Visualized Experiments   ( 151 ) e59475  2019.09  [Refereed]

    DOI

  • Salivary Antioxidants Status Following Progressive Aerobic Exercise: What Are the Differences between Waterpipe Smokers and Non-Smokers?

    Arazi, Taati, Rafati Sajedi, Suzuki

    Antioxidants   8 ( 10 ) 418 - 418  2019.09  [Refereed]

     View Summary

    Waterpipe tobacco (WPT) smoking is a public health problem with similar or even stronger effects than cigarette smoking. Although it appears to be associated with extensive oxidative stress, there is a limited number of studies on the oxidative effects of WPT smoking in stressful conditions. We, therefore, compared the responses of salivary flow rate (SFR), uric acid (UA) concentration, and peroxidase (POX) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) activities between WPT smokers and non-smokers following a bout of exhaustive aerobic exercise (AE). Twenty-three sedentary young women (age: 22.95 ± 2.83 years) participated in this study, including 11 smokers (7.00 ± 1.41 uses/week) and 12 non-smokers. All participants were required to perform the Bruce protocol treadmill test at an initial gradient of 10% at 1.7 mph, with increases of these parameters every 3 min until exhaustion. Unstimulated saliva samples were collected before, immediately after, and 1 hour after AE. WPT smokers showed lower SFR compared with non-smokers at all time points (p &lt; 0.05). In comparison to WPT smokers, a larger increase in POX activity (approximately 23% vs 14%; p = 0.009) and a smaller decline in DPPH activity (approximately −8% vs −15%; p = 0.004) were found in non-smokers compared with WPT smokers. While these changes were slowly compensated within 1 hour after exhaustion, the activity of both markers was different from the pre-exercise values (p &lt; 0.001). There was also a trend for UA concentration in non-smokers to be higher during the recovery period, with no significant difference between the groups (p &gt; 0.05). It seems that WPT smoking is associated with negative effects on important human antioxidants and a diminished antioxidative response following acute exercise.

    DOI

  • Acute Effects of Transdermal Administration of Jojoba Oil on Lipid Metabolism in Mice

    Yutaka Matsumoto, Sihui Ma, Takaki Tominaga, Keiko Yokoyama, Kanae Kitatani, Kazumasa Horikawa, Katsuhiko Suzuki

    Medicina   55 ( 9 ) 594 - 594  2019.09  [Refereed]

     View Summary

    Background and objectives: Aroma therapy is a complementary therapy using essential oils diluted with carrier oils. Jojoba oils have been widely used as carrier oils. However, limited information is available regarding their effects on blood biochemical parameters. This study aimed to investigate the effect of transdermal administration of jojoba oil on blood biochemical parameters in mice. Materials and Methods: Eight-week-old male hairless mice were randomly divided into naïve control and treatment groups. In the treatment group, mice were topically administered 4 μL of jojoba oil, per gram of body weight, on the dorsa 30 min before euthanasia. Thereafter, serum biochemical parameters were assayed, and gene expression was analyzed in various tissues via a real-time polymerase chain reaction. Results: Serum non-esterified fatty acid (NEFA) levels increased significantly 30 min after topical application of jojoba oil (p &lt; 0.05). Atgl was significantly upregulated in the liver (p &lt; 0.05), and Atgl upregulation in the liver was positively correlated with serum NEFA levels (r = 0.592, p &lt; 0.05). Furthermore, a trend of decreasing fatty acid trafficking-related gene (FABPpm, FATP-1, FATP-3, and FATP-4) expression in the skin after topical application of jojoba oil (p = 0.067, 0.074, 0.076, and 0.082, respectively) was observed. Conclusions: Serum NEFA levels were elevated 30 min after transdermal administration of jojoba oil. The mechanisms of elevated serum NEFA levels might be related to both enhanced lipolysis in the liver and reduced fatty acid trafficking in the skin.

    DOI

  • Linking What We Eat to Our Mood: A Review of Diet, Dietary Antioxidants, and Depression

    Qingyi Huang, Huan Liu, Katsuhiko Suzuki, Sihui Ma, Chunhong Liu

    Antioxidants   8 ( 9 ) 376 - 376  2019.09  [Refereed]

     View Summary

    Studies have shown that diet and nutrition play significant roles in the prevention of depression and its clinical treatment. The present review aims to provide a clear understanding of the associations between diet patterns, specific foods, nutrients such as antioxidants, and depression. As a result, balanced dietary patterns such as the Mediterranean diet and certain foods such as fish, fresh vegetables, and fruits have been associated with a lower risk of depression or depressive symptoms, while high-fat Western diets and sugar-sweetened beverages have been associated with higher risk of depression or depressive symptoms. Dietary antioxidants such as green tea polyphenols or isoflavonoid intake have been negatively associated with depression or depressive symptoms. It is concluded that diet patterns, specific foods, and antioxidants play important roles in the prevention and clinical treatment of depression.

    DOI

  • 年代別・性別によるウエスト身長比と生活習慣病有病率の関連 WASEDA'S Health Study

    田端 宏樹, 小西 真幸, 谷澤 薫平, 伊藤 智子, 川上 諒子, 薄井 澄誉子, 澤田 亨, 石井 香織, 緑川 泰史, 鳥居 俊, 鈴木 克彦, 岡 浩一朗, 樋口 満, 坂本 静男

    日本臨床運動療法学会学術集会プログラム・抄録集   38回   53 - 53  2019.08

  • Glucose Ingestion Inhibits Endurance Exercise-Induced IL-6 Producing Macrophage Infiltration in Mice Muscle

    Takaki Tominaga, Sihui Ma, Kumiko Saitou, Katsuhiko Suzuki

    Nutrients   11 ( 7 ) 1496 - 1496  2019.06  [Refereed]

     View Summary

    Background: Carbohydrate (CHO) supplementation during exercise attenuates exercise-induced increases in plasma Interleukin (IL)-6 concentration. However, the effects of CHO supplementation on muscle IL-6 production during endurance exercise is controversial. The purpose of this study was to investigate the effects of CHO supplementation on muscle IL-6 production during endurance exercise with a special focus on the IL-6 producing cells. Methods: C57BL/6J mice were divided into three groups—sedentary with water ingestion group as the control (Con; n = 10), exercise with water ingestion group (Ex; n = 10), and exercise with 6% glucose ingestion group (Ex + glucose; n = 10). The Ex and Ex + glucose groups completed 3 h of treadmill running (24 m/min, 7% incline) and were sacrificed immediately after exercise. Results: The exercise-induced increases of plasma IL-6 concentration and gastrocnemius IL-6 gene expression were attenuated by glucose ingestion. However, the increases of soleus IL-6 gene expression and gastrocnemius and soleus IL-6 protein expression were not attenuated by glucose ingestion. Furthermore, we observed that macrophages that infiltrated muscle produce IL-6 and glucose ingestion attenuated the infiltration of IL-6-producing macrophages. Conclusion: This study revealed that infiltrating macrophages may be one type of IL-6-producing cells during endurance exercise, and the infiltration of these cells in muscle was attenuated by glucose ingestion. However, the effects of glucose ingestion on muscle IL-6 production were limited.

    DOI

  • β-Hydroxy-β-Methylbutyrate Free Acid Attenuates Oxidative Stress Induced by a Single Bout of Plyometric Exercise

    Hamid Arazi, Zahra Hosseini, Abbas Asadi, Rodrigo Ramirez-Campillo, Katsuhiko Suzuki

    Frontiers in Physiology   10  2019.06  [Refereed]

    DOI

  • Chronic Inflammation as an Immunological Abnormality and Effectiveness of Exercise

    Katsuhiko Suzuki

    Biomolecules   9 ( 6 ) 223 - 223  2019.06  [Refereed]

     View Summary

    Reduced levels of physical activity in people’s daily lives cause the development of metabolic syndromes or age-related disorders. Chronic inflammation is now understood to be an underlying pathological condition in which inflammatory cells such as neutrophils and monocyte/macrophages infiltrate into fat and other tissues and accumulate when people become obese due to overeating and/or physical inactivity. Pro-inflammatory mediators such as cytokines that are secreted in excess from inflammatory cells will not only lead to the development of arteriosclerosis when they chronically affect blood vessels but also bring tissue degeneration and/or dysfunction to various organs. Chronic inflammation is also involved in sarcopenia that brings hypofunction in the elderly, dementia, osteoporosis, or cancer and negatively affects many chronic diseases and people’s healthy life expectancy. In this paper, outlines of such studies are introduced in terms of homeostatic inflammation, which occurs chronically due to the innate immune system and its abnormalities, while focusing on the efficacy of exercise from aspects of immunology and oxidative stress. The preventative effects of functional food ingredients in combination with exercise are also introduced and described. The challenges and future directions in understanding the role of exercise in the control of chronic inflammation are discussed.

    DOI

  • Exercise and Inflammation

    Llion Roberts, Katsuhiko Suzuki

    Antioxidants   8 ( 6 ) 155 - 155  2019.06  [Refereed]

     View Summary

    Exercise and inflammation induce multi-faceted physiological responses in their own right, let alone when considered together [...]

    DOI

  • Bavachin attenuates LPS-induced inflammatory response and inhibits the activation of NLRP3 inflammasome in macrophages

    Yung-Li Hung, Shu-Chi Wang, Katsuhiko Suzuki, Shih-Hua Fang, Chi-Shuo Chen, Wei-Chung Cheng, Chia-Cheng Su, Hsin-Chih Yeh, Hung-Pin Tu, Po-Len Liu, Ming-Yii Huang, Chia-Yang Li

    Phytomedicine   59   152785 - 152785  2019.06  [Refereed]

    DOI

  • 免疫機能の立場からみた疾病構造の変化と運動の有用性

    鈴木克彦

    介護予防・健康づくり   6 ( 1 ) 21 - 25  2019.06  [Refereed]

  • 自然免疫・炎症に及ぼす運動の影響-そのメカニズム.

    鈴木克彦

    医学のあゆみ   269 ( 11 ) 883 - 888  2019.06  [Invited]

  • Acute Effects of Mental Activity on Response of Serum BDNF and IGF-1 Levels in Elite and Novice Chess Players

    Hamid Arazi, Hanieh Aliakbari, Abbas Asadi, Katsuhiko Suzuki

    Medicina   55 ( 5 ) 189 - 189  2019.05  [Refereed]

     View Summary

    Background and Objectives: Although the effects of physical exercise on brain functions are well studied, the influence of mental activity is unknown. The aim of this study was to assess the influence of a session of mental activity on brain neurobiological factors in chess players. Materials and Methods: Ten elite and novice chess players were recruited to participate in this study as volunteers. The subjects performed a session of standard chess matches as a mental activity. Before and after each chess match, blood samples were drawn to analyze changes in serum brain-derived neurotrophic factor (BDNF) and insulin-like growth factor 1 (IGF-1). Results: After each chess match, both the elite and novice groups showed significant increases in serum BDNF and IGF-1 concentrations. The elite group also showed significantly greater changes in BDNF and IGF-1 levels (p ≤ 0.05) than the novice group. Conclusions: Our findings indicate that a session of standard chess matches as a mental activity is effective for elevating BDNF and IGF-1 levels, and that their elevation in elite players seems to be more pronounced than those in novice players.

    DOI

  • Effects of increased daily physical activity on mental health and depression biomarkers in postmenopausal women.

    Takahashi M, Lim PJ, Tsubosaka M, Kim HK, Miyashita M, Suzuki K, Tan EL, Shibata S

    Journal of physical therapy science   31 ( 4 ) 408 - 413  2019.04  [Refereed]  [Domestic journal]

     View Summary

    [Purpose] Little is known about the effectiveness of daily physical activity on depression biomarkers in older adults. This study aimed to investigate the effects of increased daily physical activity for 8 weeks on depression biomarkers in postmenopausal women. [Participants and Methods] Thirty-eight postmenopausal females were randomly assigned into a control or an active group and were asked to wear a uniaxial accelerometer for 8 weeks. Blood samples were obtained at baseline and at the end of the intervention. During the intervention, the active group was asked to increase their physical activity level above their usual lifestyle whereas those in the control group maintained their daily lifestyle. [Results] After the 8-week intervention, the step counts of the participants in the active group increased. The serum concentration of the brain-derived neurotrophic factor and serotonin increased significantly in the active group, but not in the control group, as compared with baseline values. The serum concentration of derivatives of reactive oxygen metabolites and biological antioxidant potential did not change after the intervention in either group. [Conclusion] These findings may suggest that promotion of daily physical activity in postmenopausal women has a positive impact on depression without any change in oxidative stress.

    DOI PubMed

  • Characterization of Exercise-Induced Cytokine Release, the Impacts on the Body, the Mechanisms and Modulations

    Katsuhiko Suzuki

    International Journal of Sports and Exercise Medicine   5 ( 3 )  2019.03  [Refereed]

    DOI

  • The Effects of Exercise and Massage on Disuse Muscle Atrophy in Special Focus on Mechanical Stress

    Kumiko Saitou, Katsuhiko Suzuki

    International Journal of Musculoskeletal Disorders   3   115  2019.03  [Refereed]

  • Keto-Adaptation and Endurance Exercise Capacity, Fatigue Recovery, and Exercise-Induced Muscle and Organ Damage Prevention: A Narrative Review

    Sihui Ma, Katsuhiko Suzuki

    Sports   7 ( 2 ) 40 - 40  2019.02  [Refereed]

     View Summary

    A ketogenic diet (KD) could induce nutritional ketosis. Over time, the body will acclimate to use ketone bodies as a primary fuel to achieve keto-adaptation. Keto-adaptation may provide a consistent and fast energy supply, thus improving exercise performance and capacity. With its anti-inflammatory and anti-oxidative properties, a KD may contribute to muscle health, thus preventing exercise-induced fatigue and damage. Given the solid basis of its potential to improve exercise capacity, numerous investigations into KD and exercise have been carried out in recent years. This narrative review aims to summarize recent research about the potential of a KD as a nutritional approach during endurance exercise, focusing on endurance capacity, recovery from fatigue, and the prevention of exhaustive exercise-induced muscle and organ damage.

    DOI

  • Beta-Cryptoxanthin Inhibits Lipopolysaccharide-Induced Osteoclast Differentiation and Bone Resorption via the Suppression of Inhibitor of NF-κB Kinase Activity

    Narumi Hirata, Ryota Ichimaru, Tsukasa Tominari, Chiho Matsumoto, Kenta Watanabe, Keita Taniguchi, Michiko Hirata, Sihui Ma, Katsuhiko Suzuki, Florian Grundler, Chisato Miyaura, Masaki Inada

    Nutrients   11 ( 2 ) 368 - 368  2019.02  [Refereed]

     View Summary

    Beta-cryptoxanthin (β-cry) is a typical carotenoid found abundantly in fruit and vegetables such as the Japanese mandarin orange, persimmon, papaya, paprika, and carrot, and exerts various biological activities (e.g., antioxidant effects). We previously reported that β-cry suppressed lipopolysaccharide (LPS)-induced osteoclast differentiation via the inhibition of prostaglandin (PG) E2 production in gingival fibroblasts and restored the alveolar bone loss in a mouse model for periodontitis in vivo. In this study, we investigated the molecular mechanism underlying the inhibitory effects of β-cry on osteoclast differentiation. In mouse calvarial organ cultures, LPS-induced bone resorption was suppressed by β-cry. In osteoblasts, β-cry inhibited PGE2 production via the downregulation of the LPS-induced mRNA expression of cyclooxygenase (COX)-2 and membrane-bound PGE synthase (mPGES)-1, which are PGE synthesis-related enzymes, leading to the suppression of receptor activator of NF-κB ligand (RANKL) mRNA transcriptional activation. In an in vitro assay, β-cry directly suppressed the activity of the inhibitor of NF-κB kinase (IKK) β, and adding ATP canceled this IKKβ inhibition. Molecular docking simulation further suggested that β-cry binds to the ATP-binding pocket of IKKβ. In Raw264.7 cells, β-cry suppressed RANKL-mediated osteoclastogenesis. The molecular mechanism underlying the involvement of β-cry in LPS-induced bone resorption may involve the ATP-competing inhibition of IKK activity, resulting in the suppression of NF-κB signaling.

    DOI

  • Exercise effects on physiological function during aging

    Zsolt Radak, Ferenc Torma, Istvan Berkes, Sataro Goto, Tatsuya Mimura, Aniko Posa, Laszlo Balogh, Istvan Boldogh, Katsuhiko Suzuki, Mitsuru Higuchi, Erika Koltai

    Free Radical Biology and Medicine   132   33 - 41  2019.02  [Refereed]

    DOI

  • Dietary patterns and abdominal obesity in middle-aged and elderly Japanese adults: Waseda Alumni's Sports, Exercise, Daily Activity, Sedentariness and Health Study (WASEDA'S Health Study).

    Tomoko Ito, Ryoko Kawakami, Kumpei Tanisawa, Rina Miyawaki, Kaori Ishii, Suguru Torii, Katsuhiko Suzuki, Shizuo Sakamoto, Isao Muraoka, Koichiro Oka, Mitsuru Higuchi

    Nutrition (Burbank, Los Angeles County, Calif.)   58   149 - 155  2019.02  [Refereed]  [International journal]

     View Summary

    OBJECTIVE: The aim of this study was to investigate the associations between dietary patterns and abdominal obesity among middle-aged and elderly Japanese people, using both waist circumference (WC) and visceral fat (VF) as indices. METHODS: A cross-sectional study was conducted with 829 adults (534 men and 295 women), 40 to 79 y of age, who participated in the Waseda Alumni's Sports, Exercise, Daily Activity, Sedentariness and Health Study (WASEDA'S Health Study). Dietary patterns were derived from principal component analysis. VF was measured using magnetic resonance imaging. To examine the associations of each dietary pattern with WC and VF, we calculated multivariate-adjusted means and 95% confidence intervals (CIs) of WC and VF for the tertile of each dietary pattern score. RESULTS: Two main dietary patterns were identified: "healthy Japanese" and "seafood and alcohol." The healthy Japanese dietary pattern score was inversely associated with WC and VF in men only. WC measurements were 84.9 cm (95% CI, 83.7-86.1), 83.9 cm (95% CI, 82.7-85.1), and 82.4 cm (95% CI, 81.2-83.6); Ptrend = 0.006, and VF measurements were 94.0 cm2 (95% CI, 85.6-102.4), 89.4 cm2 (95% CI, 81.1-97.7), and 80.4 cm2 (95% CI, 72.5-88.4); Ptrend = 0.027 for the lowest through the highest tertile of healthy Japanese dietary pattern scores in men. The seafood and alcohol dietary pattern was not associated with WC and VF. CONCLUSION: The healthy Japanese dietary pattern was negatively associated with WC and VF in middle-aged and elderly Japanese men.

    DOI PubMed

  • Exercise and probiotics attenuate the development of Alzheimer's disease in transgenic mice: Role of microbiome

    Dora Abraham, Janos Feher, Gian Luca Scuderi, Dora Szabo, Arpad Dobolyi, Melinda Cservenak, Janos Juhasz, Balazs Ligeti, Sandor Pongor, Mari Carmen Gomez-Cabrera, Jose Vina, Mitsuru Higuchi, Katsuhiro Suzuki, Istvan Boldogh, Zsolt Radak

    Experimental Gerontology   115   122 - 131  2019.01  [Refereed]

    DOI

  • 運動誘発性低血糖時の血糖変化量の再評価 安静時との比較

    近藤 早希, 谷澤 薫平, 鈴木 克彦, 寺田 新, 樋口 満

    日本スポーツ栄養研究誌   12   77 - 85  2019.01  [Refereed]

     View Summary

    【目的】運動開始30〜45分前の糖質摂取は、運動開始直後に急激な血糖値の低下を生じさせる。一方、糖質摂取後に安静状態を保った場合に比べ、血糖値がどの程度低下するのかは明らかでない。本研究では、運動誘発性の血糖値の低下が、糖質摂取後に安静状態を保った場合に比べてどの程度であるのか、実際のスポーツ現場に近い状況である朝食摂取条件下にて検討した。【方法】男子大学生15名に対し、実験開始3時間前に朝食を摂取させた状態で、運動試行、安静試行の2試行を行わせた。運動試行では、150gの糖質を摂取してから30分間安静状態を保った後、最大酸素摂取量の75%の強度で60分間自転車運動を行った。安静試行では、糖質摂取後に90分間安静状態を維持した。それぞれの試行において採血を行い、血糖値を測定した。【結果】運動試行では、運動開始15分後に急激に血糖値が低下し、安静試行に比べて28±29mg/dl低い値を示した。また、安静試行から運動試行への血糖低下量が大きい被験者は、血糖低下量が小さい被験者に比べて、最大酸素摂取量が有意に高い値を示した。【結論】朝食摂取条件下においても、糖質摂取後に運動を行うことで、同量の糖質を摂取して安静状態を保った場合に比べて、約30mg/dl程度の血糖値の低下が生じることが明らかとなった。また、血糖低下量が大きい被験者では、最大酸素摂取量が高い傾向が認められた。(著者抄録)

  • Effects of Exercise Training on Growth and Differentiation Factor 11 Expression in Aged Mice.

    Lee M, Oikawa S, Ushida T, Suzuki K, Akimoto T

    Frontiers in physiology   10   970  2019  [Refereed]

     View Summary

    Exercise training is considered an effective way to prevent age-related skeletal muscle loss. However, the molecular mechanism has not been clarified. Growth and differentiation factor 11 (GDF11) has been controversially considered a regulator of skeletal muscle aging. In this study, we examined whether GDF11 is associated with skeletal muscle aging and the effects of exercise training on age-related skeletal muscle loss. First, we observed that Gdf11 mRNA and protein expression levels in young (5-month-old, n = 6) and aged (22-to 26-month-old, n = 5) mice were not significantly different. Aged mice were then divided into sedentary (n = 5) and exercise (n = 6) groups. The exercise group performed moderate-intensity treadmill running for 6 weeks. Treadmill exercise training increased Gdf11 mRNA expression in the soleus muscle, but its protein expression was not altered. In contrast, the GDF11 level in the plantaris muscle was not changed at either the mRNA or protein level. Collectively, our data demonstrate that GDF11 levels do not change during aging, and that treadmill exercise training increased Gdf11 mRNA expression in a predominantly slow-twitch muscle.

    DOI PubMed

  • Monocyte Subsets in Atherosclerosis and Modification with Exercise in Humans

    Ning Aw, Elisa Canetti, Katsuhiko Suzuki, Jorming Goh

    Antioxidants   7 ( 12 ) 196 - 196  2018.12  [Refereed]

     View Summary

    Atherosclerosis is a progressive pathological remodeling of the arteries and one of its hallmarks is the presence of chronic inflammation. Notably, there is an increased proportion and activation state of specific monocyte subsets in systemic blood circulation. Monocyte subsets have distinct contributions to the formation, progression, and destabilization of the atherosclerotic plaque. Strong clinical and epidemiological studies show that regular aerobic exercise mitigates the progression of cardiovascular disease. In fact, aerobic fitness is a powerful predictor of cardiovascular mortality in adults, independent of traditional risk factors such as hypertension and hyperlipidemia. Acute bouts and chronic exercise training modulate monocyte behavior, ranging from their recruitment from the bone marrow or marginal pool, to tissue margination and functional changes in cytokine and chemokine production. Such modulation could reflect a potential mechanism for the cardio-protective effect of exercise on atherosclerosis. This review summarizes the current knowledge of monocyte subsets and highlights what is known about their responses to exercise.

    DOI

  • Exercise training suppresses scavenger receptor CD36 expression in kupffer cells of nonalcoholic steatohepatitis model mice

    Noriaki Kawanishi, Tsubasa Mizokami, Koichi Yada, Katsuhiko Suzuki

    Physiological Reports   6 ( 23 ) e13902 - e13902  2018.12  [Refereed]

    DOI

  • An 8-Week Ketogenic Diet Alternated Interleukin-6, Ketolytic and Lipolytic Gene Expression, and Enhanced Exercise Capacity in Mice

    Sihui Ma, Qingyi Huang, Takaki Tominaga, Chunhong Liu, Katsuhiko Suzuki

    Nutrients   10 ( 11 ) 1696 - 1696  2018.11  [Refereed]

     View Summary

    Adjusting dietary fat intake is reported to affect mitochondrial biogenesis and fatty acid oxidation (FAO), and thus may enhance exercise capacity. However, a high-fat diet where carbohydrate intake is not limited enough also makes it difficult for athletes to maintain weight, and may fail to force the body to utilize fat. As such, a low-carbohydrate, high-fat, ketogenic diet (KD) may be viable. We have previously reported that an eight-week KD enhances exercise capacity, and suggested the mechanism to be enhanced lipolysis and ketolysis. In the present study, we investigated how an eight-week KD alters mRNA expression during fatty acid mobilization, FAO and ketolysis. We found that an eight-week KD may remodel the lipid metabolism profile, thus contributing to influence exercise capacity. We also found that ketolysis, lipolysis and FAO adaptations may contribute to enhanced exhaustive exercise performance. Along with enhanced FAO capacity during exhaustive exercise, a KD may also alter IL-6 synthesis and secretion profile, thus contribute to fatty acid mobilization, ketolysis, lipolysis and preventing muscle damage. Both the lipid metabolism response and IL-6 secretion appeared to be muscle fiber specific. Taken together, the previous and present results reveal that an eight-week KD may enhance exercise performance by up-regulating ketolysis and FAO ability. Therefore, a KD may have the potential to prevent muscle damage by altering IL-6 secretion profile, indicating that a KD may be a promising dietary approach in endurance athletes, sports, and for injury prevention.

    DOI

  • DXAで推定した成人の内臓脂肪面積の妥当性 WASEDA'S Health Study(Validity of visceral fat area estimated from DXA in adults: WASEDA'S Health Study)

    Usui Chiyoko, Midorikawa Taishi, Tanisawa Kumpei, Kawakami Ryoko, Ito Tomoko, Torii Suguru, Sakamoto Shizuo, Suzuki Katsuhiko, Oka Koichiro, Higuchi Mitsuru

    The Journal of Physical Fitness and Sports Medicine   7 ( 6 ) 390 - 390  2018.11

  • A Review of the Effects of Leucine Metabolite (β-Hydroxy-β-methylbutyrate) Supplementation and Resistance Training on Inflammatory Markers: A New Approach to Oxidative Stress and Cardiovascular Risk Factors

    Hamid Arazi, Behzad Taati, Katsuhiko Suzuki

    Antioxidants   7 ( 10 ) 148 - 148  2018.10  [Refereed]

     View Summary

    β-hydroxy β-methylbutyrate (HMB) is a bioactive metabolite formed from the breakdown of the branched-chain amino acid, leucine. Given the popularity of HMB supplements among different athletes, specifically, those who participate in regular resistance training, this review was performed to summarize current literature on some aspects of HMB supplementation that have received less attention. Because of the small number of published studies, it has not been possible to conclude the exact effects of HMB on cardiovascular parameters, oxidative stress, and inflammatory markers. Thus, the interpretation of outcomes should be taken cautiously. However, the data presented here suggest that acute HMB supplementation may attenuate the pro-inflammatory response following an intense bout of resistance exercise in athletes. Also, the available findings collectively indicate that chronic HMB consumption with resistance training does not improve cardiovascular risk factors and oxidative stress markers greater than resistance training alone. Taken together, there is clearly a need for further well-designed, long-term studies to support these findings and determine whether HMB supplementation affects the adaptations induced by resistance training associated with the body’s inflammatory condition, antioxidative defense system, and cardiovascular risk factors in humans.

    DOI

  • Local cyclical compression modulates macrophage function in situ and alleviates immobilization-induced muscle atrophy

    Kumiko Saitou, Masakuni Tokunaga, Daisuke Yoshino, Naoyoshi Sakitani, Takahiro Maekawa, Youngjae Ryu, Motoshi Nagao, Hideki Nakamoto, Taku Saito, Noriaki Kawanishi, Katsuhiko Suzuki, Toru Ogata, Michiru Makuuchi, Atsushi Takashima, Keisuke Sawada, Shunsuke Kawamura, Koichi Nakazato, Karina Kouzaki, Ichiro Harada, Yoshinori Ichihara, Yasuhiro Sawada

    Clinical Science   132 ( 19 ) 2147 - 2161  2018.10  [Refereed]  [International journal]

     View Summary

    Physical inactivity gives rise to numerous diseases and organismal dysfunctions, particularly those related to aging. Musculoskeletal disorders including muscle atrophy, which can result from a sedentary lifestyle, aggravate locomotive malfunction and evoke a vicious circle leading to severe functional disruptions of vital organs such as the brain and cardiovascular system. Although the significance of physical activity is evident, molecular mechanisms behind its beneficial effects are poorly understood. Here, we show that massage-like mechanical interventions modulate immobilization-induced pro-inflammatory responses of macrophages in situ and alleviate muscle atrophy. Local cyclical compression (LCC) on mouse calves, which generates intramuscular pressure waves with amplitude of 50 mmHg, partially restores the myofiber thickness and contracting forces of calf muscles that are decreased by hindlimb immobilization. LCC tempers the increase in the number of cells expressing pro-inflammatory proteins, tumor necrosis factor-α and monocyte chemoattractant protein-1 (MCP-1), including macrophages in situ. The reversing effect of LCC on immobilization-induced thinning of myofibers is almost completely nullified when macrophages recruited from circulating blood are depleted by administration of clodronate liposomes. Furthermore, application of pulsatile fluid shear stress, but not hydrostatic pressure, reduces the expression of MCP-1 in macrophages in vitro. Together with the LCC-induced movement of intramuscular interstitial fluid detected by µCT analysis, these results suggest that mechanical modulation of macrophage function is involved in physical inactivity-induced muscle atrophy and inflammation. Our findings uncover the implication of mechanosensory function of macrophages in disuse muscle atrophy, thereby opening a new path to develop a novel therapeutic strategy utilizing mechanical interventions.

    DOI PubMed

  • Single Dose Administration of Taheebo Polyphenol Enhances Endurance Capacity in Mice

    Koichi Yada, Katsuhiko Suzuki, Natsumi Oginome, Sihui Ma, Youichi Fukuda, Akira Iida, Zsolt Radak

    Scientific Reports   8 ( 1 ) 14625 - 14625  2018.10  [Refereed]  [International journal]

     View Summary

    Endurance capacity is important for maintenance of quality of life as well as performance of endurance athletes. In order to improve endurance, intake of nutritional supplements as well as exercise training is also important. Indeed, polyphenolic extracts from plants are known to improve endurance capacity via increase of fatty acid utilization, mitochondrial biogenesis or inhibition of oxidative stress. Taheebo, the extract obtained from inner bark of Tabebuia avellanedae has been reported to have beneficial effects for treatment of inflammation, oxidative stress and obesity. Here, we investigated the effects and mechanisms of polyphenol fraction of taheebo (taheebo polyphenol; TP) on endurance capacity of mice. Single dose administration of TP significantly increased running time until exhaustion. Acute TP administration increased blood glucose and muscle glycogen levels (p < 0.05) through alteration on expression level of genes involved with glycogen metabolism and gluconeogenesis. Furthermore, TP administration decreased exercise-induced increase of protein carbonyls in skeletal muscle. These results suggest that TP administration improve endurance capacity via up-regulation of skeletal muscle glycogen levels and maintenance of blood glucose by acceleration of gluconeogenesis as well as inhibition of exercise-induced oxidative stress. Single administration of TP also increased phosphorylation of AMP-activated protein kinase (AMPK) and gene expression level of sirtuin 1 (SIRT1) but did not change the marker of mitochondrial biogenesis.

    DOI PubMed

  • An 8-Week, Low Carbohydrate, High Fat, Ketogenic Diet Enhanced Exhaustive Exercise Capacity in Mice Part 2: Effect on Fatigue Recovery, Post-Exercise Biomarkers and Anti-Oxidation Capacity

    Qingyi Huang, Sihui Ma, Takaki Tominaga, Katsuhiko Suzuki, Chunhong Liu

    Nutrients   10 ( 10 ) 1339 - 1339  2018.09  [Refereed]

     View Summary

    A low-carbohydrate, high-fat ketogenic diet (KD) is a nutritional approach ensuring that the body utilizes lipids. In our previous study, we found that an eight-week ketogenic high-fat, low-carbohydrate diet increased the capacity of endurance exercise in mice without aggravated muscle injury, despite the decrease of absolute muscle volume. The potential mechanism is most possibly to be enhanced capacity to mobilize and utilize fat. In the present study, we investigated whether a ketogenic diet influences post-exercise recovery by measuring blood biomarkers, muscle and liver oxidative state as well as fatigue recovery 24 h post exercise by employing an open-field locomotion test. Several biochemistry markers indicating exercise-induced injury after exhaustive exercise were improved by KD, followed by a 24-h rest with free feed access, including lactate. No aggravated hepatic oxidative damage was observed, whereas muscular oxidative stress was increased by KD. Accelerated recovery induced by exhaustive exercise was also observed from blood biomarkers of injury. For fatigue recovery, lactate concentration, a marker often employed as exhaustion index was lowered by KD, whereas an open field test showed that KD application contributed to increased locomotion after exhaustive exercise, followed by a 24-h rest. These results suggest that KD has the potential to be used as a fatigue-preventing and/or recovery-promoting diet approach in endurance athletes.

    DOI

  • Dietary Fiber and its Effect on Obesity: A Review Article

    Ruheea Taskin Ruhee, Katsuhiko Suzuki

    Advances in Medical Research   1  2018.09  [Refereed]

  • Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

    Takuji Kawamura, Katsuhiko Suzuki, Masaki Takahashi, Miki Tomari, Reira Hara, Yuko Gando, Isao Muraoka

    Antioxidants   7 ( 10 ) 127  2018.09  [Refereed]

    DOI

  • Preexercise Carbohydrate Ingestion and Transient Hypoglycemia: Fasting vs. Feeding.

    S. Kondo, K. Tanisawa, K. Suzuki, S. Terada, M. Higuchi

      (in press)  2018.09  [Refereed]

  • Toll-like receptor 4: Target of lipotoxicity and exercise-induced anti-inflammatory effect

    S Ma, K Suzuki

    Annals of Nutrition & Food Science   2 ( 4 ) 1027  2018.07  [Refereed]

  • Effects of aging on serum levels of lipid molecular species as determined by lipidomics analysis in Japanese men and women.

    Noriaki Kawanishi, Yuki Kato, Kyosuke Yokozeki, Shuji Sawada, Ryota Sakurai, Yoshinori Fujiwara, Shoji Shinkai, Nobuhito Goda, Katsuhiko Suzuki

    Lipids in health and disease   17 ( 1 ) 135 - 135  2018.06  [Refereed]  [International journal]

     View Summary

    BACKGROUND: Aging is known to be associated with increased risk of lipid disorders related to the development of type 2 diabetes. Recent evidence revealed that change of lipid molecule species in blood is associated with the risk of type 2 diabetes. However, changes in lipid molecular species induced by aging are still unknown. We assessed the effects of age on the serum levels of lipid molecular species as determined by lipidomics analysis. METHODS: Serum samples were collected from ten elderly men (71.7 ± 0.5 years old) and women (70.2 ± 1.0 years old), ten young men (23.9 ± 0.4 years old), and women (23.9 ± 0.7 years old). Serum levels of lipid molecular species were determined by liquid chromatography mass spectrometry-based lipidomics analysis. RESULTS: Our mass spectrometry analysis revealed increases in the levels of multiple triacylglycerol molecular species in the serum of elderly men and women. Moreover, serum levels of total ester-linked phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were increased by aging. In contrast, serum levels of specific ether-linked PC and PE molecular species were lower in elderly individuals than in young individuals. CONCLUSIONS: Our finding indicates that specific lipid molecular species, such as ether- and ester- linked phospholipids, may be selectively altered by aging.

    DOI PubMed

  • Potential Application of Ketogenic Diet to Metabolic Status and Exercise Performance: A Review

    Sihui Ma, Katsuhiko Suzuki

    EC Nutrition   13   469 - 499  2018.06  [Refereed]

  • Reactive Oxygen and Nitrogen Species Regulate Key Metabolic, Anabolic, and Catabolic Pathways in Skeletal Muscle

    Roland Nemes, Erika Koltai, Albert W. Taylor, Katsuhiko Suzuki, Ferenc Gyori, Zsolt Radak

    Antioxidants   7 ( 7 ) 85  2018.06  [Refereed]

    DOI

  • The Effects of Beta-Hydroxy-Beta-Methylbutyrate-Free Acid Supplementation and Resistance Training on Oxidative Stress Markers: A Randomized, Double-Blind, Placebo-Controlled Study

    Hamid Arazi, Abbas Asadi, Katsuhiko Suzuki

    Antioxidants   7 ( 6 ) 76  2018.06  [Refereed]

    DOI

  • Fluid intake restores retinal blood flow early after exhaustive exercise in healthy subjects.

    Tsukasa Ikemura, Katsuhiko Suzuki, Nobuhiro Nakamura, Koichi Yada, Naoyuki Hayashi

    European journal of applied physiology   118 ( 5 ) 1053 - 1061  2018.05  [Refereed]  [International journal]

     View Summary

    PURPOSE: It remains unclear whether rehydration restores retinal blood flow reduced by exhaustive exercise. We investigated the effect of fluid intake on retinal blood flow after exhaustive exercise. METHODS: Blood flow in the inferior (ITRA) and superior temporal retinal arterioles (STRA) was measured before and after incremental cycling exercise until exhaustion in 13 healthy males. After the exercise, the subjects rested without drinking (control condition: CON) or with drinking an electrolyte containing water (rehydrate condition: REH) and were followed up for a period of 120 min. To assess the hydration state, the body mass was measured, and venous blood samples were collected and plasma volume (PV) was calculated. RESULTS: Body mass decreased in CON after the trial [- 1.1 ± 0.1% (mean ± SE), p < 0.05]. PV was lower in CON than in REH during recovery. The ITRA and STRA blood flows decreased immediately after exercise from the resting baseline (ITRA; - 23 ± 4% in REH and - 30 ± 4% in CON, p < 0.05). The ITRA blood flow recovered baseline level at 15 min of recovery in REH (- 9 ± 3%, p = 0.5), but it remained reduced in CON (-14 ± 3%, p < 0.05). The STRA blood flow was higher in REH than in CON at 15 min (2 ± 3 vs. - 5 ± 3%, p < 0.05). CONCLUSIONS: The results of this study suggest that the reduction in retinal blood flow induced by exhaustive exercise can be recovered early by rehydration.

    DOI PubMed

  • The Structural Characteristics of Green Tea Polyphenols on Lipopolysaccharide-Stimulated RAW Cells

    Yung-Li Hung, Hiromi Miyazaki, Shih-Hua Fang, Chia-Yang Li, Katsuhiko Suzuki

    Journal of Nutritional Biology   4 ( 2 ) 151 - 157  2018.04  [Refereed]

     View Summary

    The inflammatory response of macrophages is involved in pathogenesis of lifestyle-related diseases. Green tea consumption reduces the incidence of lifestyle-related diseases. This study investigated the anti-inflammatory effect of polyphenols of green tea including gallic acid, (+)-catechin, (-)-catechin, (-)-epicatechin and (-) epigallocatechin-3-gallate (EGCG) in vitro. The macrophage cell line RAW264 cells were pre-treated with different concentrations of polyphenols (gallic acid, (+)-catechin, (-)-catechin, (-)-epicatechin and EGCG) for 4 h, and were stimulated with LPS for 45 min, 2 h and 24 h. After 24 h LPS challenge, cell lysates and supernatants were harvested. The protein concentration of whole cell lysate was used for determination of cell growth/viability by the BCA assay. The production of TNF-? and IL-6 was measured by ELISA. The total expression and phosphorylation of p38 MAPK was detected by Western blotting. Our results showed that the total protein content of cells was decreased after LPS challenge, while this effect was attenuated when cells were pre-treated with 10 ?M gallic acid and EGCG. Pre-treatment with 1 and 10 ?M EGCG and (-)-catechin significantly decreased the production of TNF-? and IL-6. Furthermore, pre-treatment with 10 ?M gallic acid significantly reduced the production of TNF-? and IL-6. Pre-treatment with 10 ?M (+)-catechin, (-)-catechin, (-) epicatechin, and EGCG enhanced the expression and phosphorylation of p38 MAPK after stimulation with LPS for 45 min and 2 h. By contrast, pre-treatment with gallic acid did not affect the production and phosphorylation of p38 MAPK. These results demonstrated that polyphenols with pyrogallol-type structures in green tea attenuate the activation of macrophages.

    DOI

  • Effects of Ingestion of Different Amounts of Carbohydrate after Endurance Exercise on Circulating Cytokines and Markers of Neutrophil Activation.

    Kumpei Tanisawa, Katsuhiko Suzuki, Sihui Ma, Saki Kondo, Susumu Okugawa, Mitsuru Higuchi

    Antioxidants (Basel, Switzerland)   7 ( 4 ) 51  2018.04  [Refereed]  [International journal]

     View Summary

    We aimed to examine the effects of ingestion of different amounts of carbohydrate (CHO) after endurance exercise on neutrophil count, circulating cytokine levels, and the markers of neutrophil activation and muscle damage. Nine participants completed three separate experimental trials consisting of 1 h of cycling exercise at 70% V · O₂ max, followed by ingestion of 1.2 g CHO·kg body mass-1·h-1 (HCHO trial), 0.2 g CHO·kg body mass-1·h-1 (LCHO trial), or placebo (PLA trial) during the 2 h recovery phase in random order. Circulating glucose, insulin, and cytokine levels, blood cell counts, and the markers of neutrophil activation and muscle damage were measured. The concentrations of plasma glucose and serum insulin at 1 h after exercise were higher in the HCHO trial than in the LCHO and PLA trials. Although there were significant main effects of time on several variables, including neutrophil count, cytokine levels, and the markers of neutrophil activation and muscle damage, significant time × trial interactions were not observed for any variables. These results suggest that CHO ingestion after endurance exercise does not enhance exercise-induced increase in circulating neutrophil and cytokine levels and markers of neutrophil activation and muscle damage, regardless of the amount of CHO ingested.

    DOI PubMed

  • 低強度短時間運動負荷の細胞性免疫能への影響

    鈴木克彦

    体育の科学 68 (3), 191-194, 2018   68 ( 3 ) 191 - 194  2018.04  [Invited]

  • Cytokine response to exercise and its modulation

    Katsuhiko Suzuki

    Antioxidants   7 ( 1 )  2018.03  [Refereed]

     View Summary

    Strenuous exercise induces such inflammatory responses as leukocytosis (neutrophilia) and symptoms as delayed-onset muscle soreness and swelling. However, the association between inflammatory mediator cytokines and oxidative stress is not fully delineated. Herein, in addition to basic background information on cytokines, research findings on exertional effects on cytokine release and the underlying mechanisms and triggers are introduced. Then, the associations among cytokine responses, oxidative stress, and tissue damage are described not only in overloaded skeletal muscle, but also in other internal organs. Furthermore, we introduce preventive countermeasures against the exhaustive exercise-induced pathogenesis together with the possibility of antioxidant interventions.

    DOI

  • Corrigendum: Taheebo Polyphenols Attenuate FFA-Induced Inflammation in Murine and Human Macrophage Cell Lines As Inhibitor of COX-2.

    Sihui Ma, Koichi Yada, Hyunjin Lee, Youichi Fukuda, Akira Iida, Katsuhiko Suzuki

    Frontiers in nutrition   5   2 - 2  2018  [International journal]

     View Summary

    [This corrects the article on p. 63 in vol. 4, PMID: 29312947.].

    DOI PubMed

  • Involvement of neutrophils in exercise-induced muscle damage and its modulation

    Katsuhiko Suzuki

    General Internal Medicine and Clinical Innovations   3 ( 3 ) 1 - 8  2018  [Refereed]

    DOI

  • Inflammatory Response to Exercise and Its Prevention

    Katsuhiko Suzuki

    Current Topics in Biochemical Research   19   37 - 42  2018  [Refereed]

  • Dietary patterns and abdominal obesity in middle-aged and elderly Japanese adults: The WASEDA'S Health Study

    Ito T, Kawakami R, Tanisawa K, Miyawaki R, Ishii K, Torii S, Suzuki K, Sakamoto S, Muraoka I, Oka K, Higuchi M, WASEDA'S Health, Study Group

    Nutrition   in press  2018  [Refereed]

  • Prevention of dementia onset with targeting at physical activity and social participation among Japanese community-dwelling older adults,

    Y. Nemoto, K. Suzuki

    Archives of Physical Health and Sports Medicine   1   39 - 43  2018  [Refereed]  [Invited]

  • An 8-Week Ketogenic Low Carbohydrate, High Fat Diet Enhanced Exhaustive Exercise Capacity in Mice.

    S. Ma, Q. Huang, K. Yada, C. Liu, K. Suzuki

    Nutrients   10 ( 6 ) 673  2018  [Refereed]

  • Taheebo Polyphenols Attenuate Free Fatty Acid-Induced Inflammation in Murine and Human Macrophage Cell Lines As Inhibitor of Cyclooxygenase-2

    Sihui Ma, Koichi Yada, Hyunjin Lee, Youichi Fukuda, Akira Iida, Katsuhiko Suzuki

    Frontiers in Nutrition   4   63 - 63  2017.12  [Refereed]  [International journal]

     View Summary

    Aim of study: Taheebo polyphenols (TP) are water extracts of Tabebuia spp. (Bignoniaceae), taken from the inner bark of the Tabebuia avellanedae tree, used extensively as folk medicine in Central and South America. Some anti-inflammatory drugs act by inhibiting both cyclooxygenase-2 (COX-2) and COX-1 enzymes. COX-2 syntheses prostaglandin (PG) E2, which is a species of endogenous pain-producing substance, whereas COX-1 acts as a house-keeping enzyme. Inhibiting both COX-1 and -2 simultaneously can have side effects such as gastrointestinal bleeding and renal dysfunction. Some polyphenols have been reported for its selective inhibiting activity toward COX-2 expression. Our study aimed to demonstrate the potential and mechanisms of TP as an anti-inflammation action without the side effects of COX-1 inhibition. Materials and methods: Free fatty acid-stimulated macrophage cell lines were employed to mimic macrophage behaviors during lifestyle-related diseases such as atherosclerosis and non-alcoholic steatohepatitis. Real-time polymerase chain reaction was used to detect expression of inflammatory cytokine mRNA. Griess assay was used to measure the production of nitric oxide (NO). ELISA was used to measure PG E2 production. Molecular docking was adopted to analyze the interactions between compounds from T. avellanedae and COX-2. Results: TP significantly suppressed the production of NO production, blocked the mRNA expression of iNOS, and COX-2 in both cell lines, blocked the mRNA expression of TNF-α, IL-1β, IL-6, and PGE2 in the murine cell line. However, there was no inhibitory effect on COX-1. Molecular docking result indicated that the inhibitory effects of TP on COX-2 and PGE2 could be attributed to acteoside, which is the main compound of TP that could bind to the catalytic zone of COX-2. After the interaction, catalytic ability of COX-2 is possibly inhibited, followed by which PGE2 production is attenuated. COX inhibitor screening assay showed TP as a selective inhibitor of COX-2 enzyme. Conclusion: The anti-inflammatory effects of TP can possibly regulate macrophages due to the targeted inhibition of COX-2 activity, without affecting COX-1 activity with other anti-inflammatory effects including suppression of iNOS and inflammatory cytokines. As such, TP is potentially useful in prevention and treatment of lifestyle-related disease by attenuating inflammation caused by macrophages infiltration.

    DOI PubMed

  • Effects of ?-Hydroxy-?-methylbutyrate-free Acid Supplementation on Strength, Power and Hormonal Adaptations Following Resistance Training

    Abbas Asadi, Hamid Arazi, Katsuhiko Suzuki

    NUTRIENTS   9 ( 12 )  2017.12  [Refereed]

     View Summary

    Background: -Hydroxy--methylbutyrate-free acid (HMB-FA) has been ingested prior to exercise to reduce muscle damage, however the effects of HMB-FA supplementation on hormonal, strength and power adaptation are unclear. Methods: Sixteen healthy men were matched and randomized into two groups and performed six-week resistance training while supplementing with either HMB-FA or placebo (3 g per day). The subjects were evaluated for 1 repetition maximum (1RM) bench press and leg press and vertical jump (VJ) prior to and after training intervention. In addition, blood samples were obtained before and after resistance training to evaluate resting growth hormone (GH), insulin like growth factor 1 (IGF-1), testosterone (TEST), cortisol (CORT), and adrenocorticotropic hormone (ACTH) responses. The HMB-FA supplementation group showed greater gains compared with the placebo group in peak power (effect size ES = 0.26 vs. 0.01) and 1RM leg press (ES = 1.52 vs. 0.96). In addition, the HMB-FA supplementation group indicated greater decrements in ACTH and CORT responses to training in comparison to the placebo group (p &lt; 0.05). Likewise, in GH (ES = 1.41 vs. 0.12) and IGF-1 (ES = 0.83 vs. 0.41), the HMB-FA indicated greater training effects when compared with the placebo group. Conclusions: These findings provide further support for the potential anabolic benefits associated with HMB-FA supplementation.

    DOI

  • The pattern recognition receptors and lipopolysaccharides (LPS)-induced systemic inflammation

    YL Hung, K. Suzuki

    International Journal of Research Studies in Medical and Health Sciences   2 ( 7 ) 1 - 7  2017.09  [Refereed]

  • Detection of titin fragments in urine in response to exercise-induced muscle damage

    Kazue Kanda, Jun Sakuma, Takayuki Akimoto, Yasuo Kawakami, Katsuhiko Suzuki

    PLOS ONE   12 ( 7 )  2017.07  [Refereed]

     View Summary

    Many studies have attempted to determine the associations between blood biomarkers and exercise-induced muscle damage. However, poor correlations between the changes in biomarker levels and the magnitude of muscle symptoms have been reported. Recent advances in proteomic tools offer a strategy for the comprehensive analysis of protein expression, which can be used to identify biomarkers. Here, we used a proteomic analysis to identify urinary proteins that appear in response to a calf-raise exercise, including repetitive eccentric muscle contractions, and found that a titin (also known as connectin) N-terminal fragment molecule appears in the urine after eccentric exercise. We measured the titin fragment in urine samples from nine individuals before and after eccentric exercise using a newly-established enzyme-linked immunosorbent assay and found that the titin fragment excretion rate increased 96 h after the exercise (5.1 to 77.6 pg/min, p &lt; 0.01). The changes in the titin fragment excretion rate were correlated strongly with blood markers of muscle damage and with muscle symptoms. These findings suggest that the urinary titin fragment is potentially a noninvasive biomarker of muscle damage.

    DOI

  • Corylin protects LPS-induced sepsis and attenuates LPS-induced inflammatory response.

    Yung-Li Hung, Shih-Hua Fang, Shu-Chi Wang, Wei-Chung Cheng, Po-Len Liu, Chia-Cheng Su, Chi-Shuo Chen, Ming-Yii Huang, Kuo-Feng Hua, Kun-Hung Shen, Yu-Ting Wang, Katsuhiko Suzuki, Chia-Yang Li

    Scientific reports   7   46299 - 46299  2017.04  [Refereed]  [International journal]

     View Summary

    Corylin is a main compound isolated from Psoralea corylifolia L. (Fabaceae). A variety of pharmacological effects such as antioxidant, anti-proliferation, and anti-inflammatory properties of corylin have been reported. Nevertheless, the effect of corylin in microbial infection and sepsis remains unclear. In the present study, we investigated the anti-inflammatory effects of corylin. Our experimental results demonstrated that corylin inhibited the production of TNF-α, IL-6 and NO by both LPS-activated RAW 264.7 cells and LPS-activated murine peritoneal macrophages. Moreover, corylin suppressed the expression levels of iNOS and COX-2, reduced the production of PGE2 and HMGB1, blocked the translocation of HMGB1 from the nucleus to cytosol, and decreased the phosphorylation of MAPKs in LPS-activated RAW 264.7 cells as well as suppressed the activity of NF-κB in LPS-activated J-Blue cells. In addition, the administration of corylin reduced the production of NO and TNF-α, decreased LPS-induced liver damage markers (AST and ALT) and kidney damage markers (BUN and CRE), attenuated infiltration of inflammatory cells and tissue damage of lung, liver and kidney, and enhanced the survival rate of LPS-challenged mice. Taken together, these results show the anti-inflammatory properties of corylin on LPS-induced inflammation and sepsis. Corylin could potentially be a novel anti-inflammatory and immunosuppressive drug candidate in the treatment of sepsis and septic shock.

    DOI PubMed

  • The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise

    Jonathan M. Peake, Llion A. Roberts, Vandre C. Figueiredo, Ingrid Egner, Simone Krog, Sigve N. Aas, Katsuhiko Suzuki, James F. Markworth, Jeff S. Coombes, David Cameron-Smith, Truls Raastad

    JOURNAL OF PHYSIOLOGY-LONDON   595 ( 3 ) 695 - 711  2017.02  [Refereed]

     View Summary

    Cold water immersion and active recovery are common post-exercise recovery treatments. However, little is known about whether these treatments influence inflammation and cellular stress in human skeletal muscle after exercise. We compared the effects of cold water immersion versus active recovery on inflammatory cells, pro-inflammatory cytokines, neurotrophins and heat shock proteins (HSPs) in skeletal muscle after intense resistance exercise. Nine active men performed unilateral lower-body resistance exercise on separate days, at least 1 week apart. On one day, they immersed their lower body in cold water (10 degrees C) for 10 min after exercise. On the other day, they cycled at a low intensity for 10 min after exercise. Muscle biopsies were collected from the exercised leg before, 2, 24 and 48 h after exercise in both trials. Exercise increased intramuscular neutrophil and macrophage counts, MAC1 and CD163 mRNA expression (P &lt; 0.05). Exercise also increased IL1 beta, TNF, IL6, CCL2, CCL4, CXCL2, IL8 and LIF mRNA expression (P &lt; 0.05). As evidence of hyperalgesia, the expression of NGF and GDNF mRNA increased after exercise (P &lt; 0.05). The cytosolic protein content of alpha B-crystallin and HSP70 decreased after exercise (P &lt; 0.05). This response was accompanied by increases in the cytoskeletal protein content of alpha B-crystallin and the percentage of type II fibres stained for alpha B-crystallin. Changes in inflammatory cells, cytokines, neurotrophins and HSPs did not differ significantly between the recovery treatments. These findings indicate that cold water immersion is no more effective than active recovery for reducing inflammation or cellular stress in muscle after a bout of resistance exercise.

    DOI

  • Systemic Inflammation Mediates the Effects of Endotoxemia in the Mechanisms of Heat Stroke

    Chin Leong Lim, Katsuhiko Suzuki

    Biology and Medicine   09 ( 01 )  2017  [Refereed]

    DOI

  • Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

    Suzuki K

    Journal of Nanomedicine & Biotherapeutic Discovery   07 ( 02 )  2017  [Refereed]

    DOI

  • Aloe Metabolites Prevent LPS-Induced Sepsis and Inflammatory Response by Inhibiting Mitogen-Activated Protein Kinase Activation.

    Chia-Yang Li, Katsuhiko Suzuki, Yung-Li Hung, Meng-Syuan Yang, Chung-Ping Yu, Shiuan-Pey Lin, Yu-Chi Hou, Shih-Hua Fang

    The American journal of Chinese medicine   45 ( 4 ) 847 - 861  2017  [Refereed]  [International journal]

     View Summary

    Aloe, a polyphenolic anthranoid-containing Aloe vera leaves, is a Chinese medicine and a popular dietary supplement worldwide. In in vivo situations, polyphenolic anthranoids are extensively broken down into glucuronides and sulfate metabolites by the gut and the liver. The anti-inflammatory potential of aloe metabolites has not been examined. The aim of this study was to investigate the anti-inflammatory effects of aloe metabolites from in vitro (lipopolysaccharides (LPS)-activated RAW264.7 macrophages) and ex vivo (LPS-activated peritoneal macrophages) to in vivo (LPS-induced septic mice). The production of proinflammatory cytokines (TNF-[Formula: see text] and IL-12) and NO was determined by ELISA and Griess reagents, respectively. The expression levels of iNOS and MAPKs were analyzed by Western blot. Our results showed that aloe metabolites inhibited the expression of iNOS, decreased the production of TNF-[Formula: see text], IL-12, and NO, and suppressed the phosphorylation of MAPKs by LPS-activated RAW264.7 macrophages. In addition, aloe metabolites reduced the production of NO, TNF-[Formula: see text] and IL-12 by murine peritoneal macrophages. Furthermore, aloe administration significantly reduced the NO level and exhibited protective effects against sepsis-related death in LPS-induced septic mice. These results suggest that aloe metabolites exerted anti-inflammatory effects in vivo, and that these effects were associated with the inhibition of inflammatory mediators. Therefore, aloe could be considered an effective therapeutic agent for the treatment of sepsis.

    DOI PubMed

  • Associations between sleep quality and inflammatory markers in patients with schizophrenia

    Shih-Hua Fang, Katsuhiko Suzuki, Chin Leong Lim, Ming -Shun Chung, Po-Wen Ku, Li-Jung Chen

    PSYCHIATRY RESEARCH   246   154 - 160  2016.12  [Refereed]

     View Summary

    Sleep disorder is a risk factor for several systemic inflammation-related diseases and there are extensive data showing that schizophrenia is associated with chronic low-grade systemic inflammation. This study investigated the associations between sleep quality and inflammatory markers in patients with schizophrenia, which has not been examined before. Sleep quality (total sleep time, sleep efficiency, sleep onset latency, total activity counts, wake after sleep onset, number of awakening, and average length of awakening) was measured using actigraphy in 199 schizophrenia inpatients. The state of inflammation was measured using blood concentration of white blood cells (WBC) and neutrophils, together with neutrophil lymphocyte ratio (NLR), and platelet lymphocyte ratio (PLR). The results showed that total sleep time was negatively associated with NLR and PLR, and sleep efficiency was negatively associated with neutrophil counts and NLR. Sleep onset latency, total activity counts, wake after sleep onset, and number of awakening were positively associated with WBC and neutrophil counts. The average length of awakening was positively associated with NLR and PLR. This is the first report to suggest that improving sleep quality may modulate the state of inflammation in patients with schizophrenia.

    DOI

  • Neutrophil Depletion Attenuates Muscle Injury after Exhaustive Exercise

    Noriaki Kawanishi, Tsubasa Mizokami, Hiroyuki Niihara, Koichi Yada, Katsuhiko Suzuki

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   48 ( 10 ) 1917 - 1924  2016.10  [Refereed]

     View Summary

    Purpose The infiltration of macrophages in skeletal muscle during exhaustive exercise promotes inflammation, myofiber lesion, and muscle injury. Although neutrophils upregulate macrophage infiltration in skeletal muscles during exercise, the role of neutrophils in promoting muscle injury after exhaustive exercise remains unclear. In this study, we investigated the effects of preexercise neutrophil depletion with antineutrophil antibody treatment on muscle injury, inflammation, and macrophage infiltration after exhaustive exercise.
    Methods Male C57BL/6J mice were randomly assigned to four groups, namely, sedentary with control antibody (n = 10), sedentary with antineutrophil antibody (n = 10), exhaustive exercise with control antibody (n = 10), and exhaustive exercise with antineutrophil antibody (n = 10). The mice were given intraperitoneal injection of the antineutrophil antibody (anti-Ly-6G, clone 1A8) or the control antibody (anti-Ly-6G, clone 2A3), and remained inactive or performed exhaustive exercise on a treadmill 48 h after the injection. Twenty-four hours after the exhaustive exercise, the gastrocnemius muscles were removed for histological and polymerase chain reaction (PCR) analyses. Infiltration of neutrophils and macrophages was evaluated with Ly-6G and F4/80 immunohistochemistry staining procedures. Muscle fiber injury was detected based on the number of IgG staining fiber. The mRNA expression levels of proinflammatory cytokines and chemokines were evaluated with real-time reverse transcription PCR.
    Results Exhaustive exercise increased neutrophil infiltration into the gastrocnemius muscle substantially by 3.1-fold and caused muscle injury, but these effects were markedly suppressed by preexercise treatment with antineutrophil antibody (neutrophil infiltration, 0.42-fold, and muscle injury, 0.18-fold). Treatment with antineutrophil antibody also decreased macrophage infiltration (0.44-fold) and mRNA expression of tumor necrosis factor- (0.55-fold) and interleukin-6 (0.51-fold) in the skeletal muscle after exhaustive exercise.
    Conclusion These results suggest that neutrophils contribute to exacerbating muscle injury by regulating inflammation through the induction of macrophage infiltration.

    DOI

  • NEW MOUSE MODEL OF SKELETAL MUSCLE ATROPHY USING SPIRAL WIRE IMMOBILIZATION

    Akiko Onda, Hajime Kono, Qibin Jiao, Takayuki Akimoto, Toshikazu Miyamoto, Yasuhiro Sawada, Katsuhiko Suzuki, Yoichiro Kusakari, Susumu Minamisawa, Toru Fukubayashi

    MUSCLE & NERVE   54 ( 4 ) 788 - 791  2016.10  [Refereed]

     View Summary

    Introduction: Disuse-induced skeletal muscle atrophy is a serious concern; however, there is not an effective mouse model to elucidate the molecular mechanisms. We developed a noninvasive atrophy model in mice. Methods: After the ankle joints of mice were bandaged into a bilateral plantar flexed position, either bilateral or unilateral hindlimbs were immobilized by wrapping in bonsai steel wire. Results: After 3, 5, or 10 days of immobilization of the hip, knee, and ankle, the weight of the soleus and plantaris muscles decreased significantly in both bilateral and unilateral immobilization. MAFbx/atrogin-1 and MuRF1 mRNA was found to have significantly increased in both muscles, consistent with disuse-induced atrophy. Notably, the procedure did not result in either edema or necrosis in the fixed hindlimbs. Conclusions: This method allows repeated, direct access to the immobilized muscle, making it a useful procedure for concurrent application and assessment of various therapeutic interventions.

    DOI PubMed

  • Neutrophil Depletion Attenuates Muscle Injury after Exhaustive Exercise

    Noriaki Kawanishi, Tsubasa Mizokami, Hiroyuki Niihara, Koichi Yada, Katsuhiko Suzuki

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   48 ( 10 ) 1917 - 1924  2016.10  [Refereed]

     View Summary

    Purpose The infiltration of macrophages in skeletal muscle during exhaustive exercise promotes inflammation, myofiber lesion, and muscle injury. Although neutrophils upregulate macrophage infiltration in skeletal muscles during exercise, the role of neutrophils in promoting muscle injury after exhaustive exercise remains unclear. In this study, we investigated the effects of preexercise neutrophil depletion with antineutrophil antibody treatment on muscle injury, inflammation, and macrophage infiltration after exhaustive exercise.
    Methods Male C57BL/6J mice were randomly assigned to four groups, namely, sedentary with control antibody (n = 10), sedentary with antineutrophil antibody (n = 10), exhaustive exercise with control antibody (n = 10), and exhaustive exercise with antineutrophil antibody (n = 10). The mice were given intraperitoneal injection of the antineutrophil antibody (anti-Ly-6G, clone 1A8) or the control antibody (anti-Ly-6G, clone 2A3), and remained inactive or performed exhaustive exercise on a treadmill 48 h after the injection. Twenty-four hours after the exhaustive exercise, the gastrocnemius muscles were removed for histological and polymerase chain reaction (PCR) analyses. Infiltration of neutrophils and macrophages was evaluated with Ly-6G and F4/80 immunohistochemistry staining procedures. Muscle fiber injury was detected based on the number of IgG staining fiber. The mRNA expression levels of proinflammatory cytokines and chemokines were evaluated with real-time reverse transcription PCR.
    Results Exhaustive exercise increased neutrophil infiltration into the gastrocnemius muscle substantially by 3.1-fold and caused muscle injury, but these effects were markedly suppressed by preexercise treatment with antineutrophil antibody (neutrophil infiltration, 0.42-fold, and muscle injury, 0.18-fold). Treatment with antineutrophil antibody also decreased macrophage infiltration (0.44-fold) and mRNA expression of tumor necrosis factor- (0.55-fold) and interleukin-6 (0.51-fold) in the skeletal muscle after exhaustive exercise.
    Conclusion These results suggest that neutrophils contribute to exacerbating muscle injury by regulating inflammation through the induction of macrophage infiltration.

    DOI

  • Physical exercise, reactive oxygen species and neuroprotection

    Zsolt Radak, Katsuhiko Suzuki, Mitsuru Higuchi, Laszlo Balogh, Istvan Boldogh, Erika Koltai

    FREE RADICAL BIOLOGY AND MEDICINE   98   187 - 196  2016.09  [Refereed]

     View Summary

    Regular exercise has systemic beneficial effects, including the promotion of brain function. The adaptive response to regular exercise involves the up-regulation of the enzymatic antioxidant system and modulation of oxidative damage. Reactive oxygen species (ROS) are important regulators of cell signaling. Exercise, via intensity-dependent modulation of metabolism and/or directly activated ROS generating enzymes, regulates the cellular redox state of the brain. ROS are also involved in the self-renewal and differentiation of neuronal stem cells and the exercise-mediated neurogenesis could be partly associated with ROS production. Exercise has strong effects on the immune system and readily alters the production of cytokines. Certain cytokines, especially IL-6, IL-1, TNF-alpha, IL-18 and IFN gamma, are actively involved in the modulation of synaptic plasticity and neurogenesis. Cytokines can also contribute to ROS production. ROS-mediated alteration of lipids, protein, and DNA could directly affect brain function, while exercise modulates the accumulation of oxidative damage. Oxidative alteration of macromolecules can activate signaling processes, membrane remodeling, and gene transcription. The well known neuroprotective effects of exercise are partly due to redox-associated adaptation. (C) 2016 Elsevier Inc. All rights reserved.

    DOI

  • The effect of the menstrual cycle and water consumption on physiological responses during prolonged exercise at moderate intensity in hot conditions

    Hideki Hashimoto, Toshimichi Ishijima, Katsuhiko Suzuki, Mitsuru Higuchi

    JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS   56 ( 9 ) 951 - 960  2016.09  [Refereed]

     View Summary

    BACKGROUND: Reproductive hormones are likely to be involved in thermoregulation through body fluid dynamics. In the present study, we aimed to investigate the effect of the menstrual cycle and water consumption on physiological responses to prolonged exercise at moderate intensity in hot conditions.
    METHODS: Eight healthy young women with regular menstrual cycles performed cycling exercise for 90 minutes at 50% VO2peak intensity during the low progesterone (LP) level phase and high progesterone (HP) level phase, with or without water consumption, under hot conditions (30 degrees C, 50% relative humidity). For the water consumption trials, subjects ingested water equivalent to the loss in body weight that occurred in the earlier non-consumption trial. For all four trials, rectal temperature, cardiorespiratory responses, and ratings of perceived exertion (RPE) were measured.
    RESULTS: Throughout the 90-minute exercise period, rectal temperatures during HP were higher than during LP by an average of 0.4 degrees C in the non-consumption trial (P&lt; 0.01) and 0.2 degrees C in the water consumption trial (P&lt; 0.05). During exercise, water consumption affected the changes in rectal temperature and heat rate (HR) during HP, but it did not exert these effects during LP. Furthermore, we found a negative correlation between estradiol levels and rectal temperature during LP.
    CONCLUSIONS: During prolonged exercise at moderate intensity under hot conditions, water consumption is likely to be useful for suppressing the associated increase in body temperature and HR, particularly during HP, whereas estradiol appears to be useful for suppressing the increase in rectal temperature during LP.

  • Macrophage depletion by clodronate liposome attenuates muscle injury and inflammation following exhaustive exercise

    Noriaki Kawanishi, Tsubasa Mizokami, Hiroyuki Niihara, Koichi Yada, Katsuhiko Suzuki

    Biochemistry and Biophysics Reports   5   146 - 151  2016.03  [Refereed]  [International journal]

     View Summary

    Exhaustive exercise promotes muscle injury, including myofiber lesions; however, its exact mechanism has not yet been elucidated. In this study, we tested the hypothesis that macrophage depletion by pretreatment with clodronate liposomes alters muscle injury and inflammation following exhaustive exercise. Male C57BL/6J mice were divided into four groups: rest plus control liposome (n=8), rest plus clodronate liposome (n=8), exhaustive exercise plus control liposome (n=8), and exhaustive exercise plus clodronate liposome (n=8). Mice were treated with clodronate liposome or control liposome for 48 h before undergoing exhaustive exercise on a treadmill. Twenty-four hours after exhaustive exercise, the gastrocnemius muscles were removed for histological and PCR analyses. Exhaustive exercise increased the number of macrophages in the muscle; however, clodronate liposome treatment reduced this infiltration. Although exhaustive exercise resulted in an increase in injured myofibers, clodronate liposome treatment following exhaustive exercise reduced the injured myofibers. Clodronate liposome treatment also decreased the mRNA expression levels of inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the skeletal muscle after exhaustive exercise. These results suggest that macrophages play a critical role in increasing muscle injury by regulating inflammation.

    DOI PubMed

  • Genome-Wide Analysis of Acute Endurance Exercise-Induced Translational Regulation in Mouse Skeletal Muscle

    Hiroaki Sako, Koichi Yada, Katsuhiko Suzuki

    PLOS ONE   11 ( 2 )  2016.02  [Refereed]

     View Summary

    Exercise dynamically changes skeletal muscle protein synthesis to respond and adapt to the external and internal stimuli. Many studies have focused on overall protein synthesis to understand how exercise regulates the muscular adaptation. However, despite the probability that each gene transcript may have its own unique translational characteristics and would be differentially regulated at translational level, little attention has been paid to how exercise affects translational regulation of individual genes at a genome-wide scale. Here, we conducted a genome-wide translational analysis using ribosome profiling to investigate the effect of a single bout of treadmill running (20 m/min for 60 min) on mouse gastrocnemius. Global translational profiles largely differed from those in transcription even at a basal resting condition as well as immediately after exercise. As for individual gene, Slc25a25 (Solute carrier family 25, member 25), localized in mitochondrial inner membrane and maintaining ATP homeostasis and endurance performance, showed significant up-regulation at translational level. However, multiple regression analysis suggests that Slc25a25 protein degradation may also have a role in mediating Slc25a25 protein abundance in the basal and early stages after acute endurance exercise.

    DOI

  • Exercise-Induced Inflammation during Different Phases of the Menstrual Cycle

    Harumi Hayashida, Mayura Shimura, Kaoru Sugama, Kazue Kanda, Katsuhiko Suzuki

    Journal of Physiotherapy & Physical Rehabilitation   01 ( 04 )  2016  [Refereed]

    DOI

  • Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness

    Takuji Kawamura, Yuko Gando, Masaki Takahashi, Reira Hara, Katsuhiko Suzuki, Isao Muraoka

    Japanese Journal of Physical Fitness and Sports Medicine   65 ( 3 ) 297 - 305  2016  [Refereed]

     View Summary

    Several studies have reported that molecular hydrogen (H2) acts as a therapeutic medical gas owing to scavenging reactive oxygen species (ROS). However, little is known about effects of H2 on exercise-induced oxidative stress. The purpose of this study was to investigate the effects of weekly hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness (DOMS). Nine healthy and active young men participated in this study, and each subject performed hydrogen bathing trial and placebo bathing trial in a crossover design. The subjects performed downhill running (8 % decline) at 75 % peak oxygen uptake (VO2peak) for 30 min, and each subjects conducted hydrogen or placebo bathing for 20 min, respectively, 1-6 days after downhill running. Before and after exercise, we measured visual analogue scale (VAS) and collected blood samples (Pre-and 5 min, 60 min after the end of bathing, 1day, 2days, 3days, 7days after downhill running). Blood sample analyses include creatine kinase (CK), myoglobin (Mb), malondialdehyde (MDA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), myeloperoxidase (MPO), interleukin-6 (IL-6), interleukin-17a (IL-17a) and lactate concentrations. Weekly hydrogen bathing had no effects of exercise-induced oxidative stress and muscle damage. On the other hand, hydrogen bathing significantly reduced DOMS (VAS) 1 and 2days after downhill running (p=0.033). These findings suggest that hydrogen bath after downhill exercise can be effective for reduction of DOMS.

    DOI CiNii

  • 運動時の酸化ストレスおよび遅発性筋痛に及ぼす水素の影響

    河村 拓史, 丸藤 祐子, 高橋 将記, 原 怜来, 鈴木 克彦, 村岡 功

    体力科学   64 ( 6 ) 600 - 600  2015.12

  • The acute effects of green tea and carbohydrate coingestion on systemic inflammation and oxidative stress during sprint cycling

    Katsuhiko Suzuki, Masaki Takahashi, Chia-Yang Li, Shiuan-Pey Lin, Miki Tomari, Cecilia M. Shing, Shih-Hua Fang

    APPLIED PHYSIOLOGY NUTRITION AND METABOLISM   40 ( 10 ) 997 - 1003  2015.10  [Refereed]

     View Summary

    Green tea (Camellia sinensis) has anti-oxidative and anti-inflammatory effects, which may be beneficial to athletes performing high-intensity exercise. This study investigated the effects of carbohydrate and green tea coingestion on sprint cycling performance and associated oxidative stress and immunoendocrine responses to exercise. In a crossover design, 9 well-trained male cyclists completed 3 sets of 8 repetitions of 100-m uphill sprint cycling while ingesting green tea and carbohydrate (TEA) (22 mg/kg body mass catechins, 6 mg/kg body mass caffeine, 230 mg/kg glucose, and 110 mg/kg fructose) or carbohydrate only (CHO) (230 mg/kg body mass glucose and 110 mg/kg body mass fructose) during each 10-min recovery period between sets. Blood samples were collected before exercise, 10 min after exercise, and 14 h after exercise. There was no effect of acute TEA ingestion on cycling sprint performance (p = 0.29), although TEA maintained postexercise testosterone and lymphocyte concentrations, which decreased significantly in the CHO group (p &lt; 0.001). While there was a trend for lower postexercise neutrophil count with TEA (p = 0.05), there were no significant differences between TEA and CHO for circulating cytokines (p &gt; 0.20), markers of oxidative stress and antioxidant capacity (p &gt; 0.17), adiponectin concentration (p = 0.60), or muscle damage markers (p &gt; 0.64). While acute green tea ingestion prevents the postexercise decrease in testosterone and lymphocytes, it does not appear to benefit cycling sprint performance or reduce markers of oxidation and inflammation when compared with carbohydrate alone.

    DOI

  • Salivary Immuno Factors, Cortisol and Testosterone Responses in Athletes of a Competitive 5,000 m Race (vol 58, pg 263, 2015)

    C. Y. Li, G. S. Hsu, K. Suzuki

    CHINESE JOURNAL OF PHYSIOLOGY   58 ( 5 ) 357 - 357  2015.10  [Refereed]

    DOI

  • Effects of vitamin C supplementation on low-intensity prolonged exercise training-induced changes of metabolic and antioxidant enzyme activities in rat skeletal muscle

    YADA Koichi, SUZUKI Katsuhiko, MATOBA Hideki

    Journal of Exercise and Sports Physiology   22 ( 2 ) 71 - 79  2015.10  [Refereed]

     View Summary

    The purpose of this study was to determine the effects of vitamin C supplementation on low-intensity prolonged exercise training-induced changes of metabolic and antioxidant enzyme activities in rat skeletal muscle. Male Sprague-Dawley rats were assigned to one of four groups: a control group, exercise training group, vitamin C supplemented group, and vitamin C supplemented exercise training group. Rats in the vitamin C supplemented groups were given vitamin C (500 mg/kg/day). The vitamin C supplemented animals were given vitamin C in their drinking water during a 2-week pretraining period. The rats in the vitamin C supplemented groups were administered vitamin C with a feeding needle 1h before each training session in the training period. Rats in the exercise training groups swam without a load for 6 h in two 3 h bouts, separated by 45 min of rest. The rats performed the training once a day for 10 days. Vitamin C supplementation attenuated oxidative stress and increased glutathione level and catalase activity. Exercise training increased citrate synthase and hexokinase activities. However, vitamin C did not change these enzyme activities. These results suggest that vitamin C does not prevent the training-induced activation of metabolic enzymes, but attenuates oxidative stress via induction of antioxidants in skeletal muscle.

    CiNii

  • Exercise training attenuates neutrophil infiltration and elastase expression in adipose tissue of high-fat-diet-induced obese mice.

    Noriaki Kawanishi, Hiroyuki Niihara, Tsubasa Mizokami, Koichi Yada, Katsuhiko Suzuki

    Physiological reports   3 ( 9 )  2015.09  [International journal]

     View Summary

    The innate immune system is associated with the development of local inflammation. Neutrophils play an essential role in the development of the adipose tissue (AT) inflammation associated with obesity by producing elastase, which can promote the activation and infiltration of macrophages. Exercise training attenuates AT inflammation via suppression of macrophage infiltration. However, the mechanisms driving this phenomenon remains to be elucidated. Here, we evaluated the effects of exercise training on the infiltration of neutrophils and elastase expression in an obese mouse model. Four-week-old male C57BL/6J mice were randomly assigned to one of three groups that either received a normal diet (ND) plus sedentary activity (n = 15), a high-fat diet (HFD) plus sedentary activity (n = 15), or a HFD plus exercise training (n = 15). Mice were fed the ND or HFD from the age of 4 weeks until 20 weeks. Mice in the exercise group ran on a treadmill for 60 min/day, 5 days/week over the same experimental period. Mice fed with the HFD had increased content of macrophages in the AT and increased inflammatory cytokine mRNA levels, which were reduced by exercise training. Similarly, AT from the HFD sedentary mice contained more neutrophils than AT from the ND mice, and the amount of neutrophils in this tissue in HFD-fed mice was lowered by exercise training. The mRNA levels of neutrophil elastase in AT were lower in the HFD exercise-trained mice than those in the HFD sedentary mice. These results suggest that exercise training plays a critical role in reducing macrophage infiltration and AT inflammation by regulating the infiltration of neutrophils.

    DOI PubMed

  • Effects of Acute Endurance Exercise Performed in the Morning and Evening on Inflammatory Cytokine and Metabolic Hormone Responses

    Hyeon-Ki Kim, Masayuki Konishi, Masaki Takahashi, Hiroki Tabata, Naoya Endo, Shigeharu Numao, Sun-Kyoung Lee, Young-Hak Kim, Katsuhiko Suzuki, Shizuo Sakamoto

    PLOS ONE   10 ( 9 )  2015.09  [Refereed]

     View Summary

    Purpose
    To compare the effects of endurance exercise performed in the morning and evening on inflammatory cytokine responses in young men.
    Methods
    Fourteen healthy male participants aged 24.3 +/- 0.8 years (mean +/- standard error) performed endurance exercise in the morning (0900-1000 h) on one day and then in the evening (1700-1800 h) on another day with an interval of at least 1 week between each trial. In both the morning and evening trials, the participants walked for 60 minutes at approximately 60% of the maximal oxygen uptake ((V) over dotO(2max)) on a treadmill. Blood samples were collected to determine hormones and inflammatory cytokines at pre-exercise, immediately post exercise, and 2 h post exercise.
    Results
    Plasma interleukin (IL)-6 and adrenaline concentrations were significantly higher immediately after exercise in the evening trial than in the morning trial (P &lt; 0.01, both). Serum free fatty acids concentrations were significantly higher in the evening trial than in the morning trial at 2 h after exercise (P &lt; 0.05). Furthermore, a significant correlation was observed between the levels of IL-6 immediately post-exercise and free fatty acids 2 h post-exercise in the evening (r = 0.68, P &lt; 0.01).
    Conclusions
    These findings suggest that the effect of acute endurance exercise in the evening enhances the plasma IL-6 and adrenaline concentrations compared to that in the morning. In addition, IL-6 was involved in increasing free fatty acids, suggesting that the evening is more effective for exercise-induced lipolysis compared with the morning.

    DOI

  • Relationship of Cardiorespiratory Fitness and Obesity Genes to Metabolic Syndrome in Adult Japanese Men

    Sanada K, Iemitsu M, Murakami H, Kawakami R, Gando Y, Kawano H, Suzuki K, Higuchi M, Miyachi M

    Physical Activity, Exercise, Sedentary Behavior and Health     171 - 192  2015.08

    DOI

  • Effect of exercise-induced muscle damage on muscle hardness evaluated by ultrasound real-time tissue elastography

    Osamu Yanagisawa, Jun Sakuma, Yasuo Kawakami, Katsuhiko Suzuki, Toru Fukubayashi

    SPRINGERPLUS   4   1 - 9  2015.07  [Refereed]

     View Summary

    Purpose: To assess the effect of exercise-induced muscle damage on muscle hardness and evaluate the relationship between muscle hardness and muscle damage indicators.
    Methods: Seven men (mean 25.3 years; 172.7 cm; 66.8 kg) performed the single-leg ankle plantar flexion exercise involving both concentric and eccentric contractions (10 sets of 40 repetitions). The hardness of the medial gastrocnemius (MG) was evaluated using ultrasound real-time tissue elastography before, from day 1 to 4, and day 7 after exercise. The strain ratio between the MG and a reference material was calculated. Simultaneously, we evaluated the magnetic resonance T2 value (an index of edema) of the triceps surae, the ankle dorsiflexion range of motion (ROM), and calf muscle soreness. Serum creatine kinase activity was assessed before, 2 and 4 h, and from day 1 to 4 after exercise.
    Results: The MG showed lower strain ratio, indicating increased muscle hardness, on day 4 post-exercise (P &lt; 0.01) and higher T2 values on days 1-7 post-exercise (P &lt; 0.01) relative to each pre-exercise value. The ankle dorsiflexion ROM was lower on days 2-4 post-exercise (P &lt; 0.01). The serum creatine kinase markedly increased on days 3 and 4 post-exercise (not significant). The degree of muscle soreness among the post-exercise time points was similar. The decreased strain ratio did not correlate with the increased T2, the decreased joint ROM or muscle soreness.
    Conclusion: Muscle hardness increased after strenuous resistance exercise, but the change was not related with muscle edema, decreased joint ROM, or muscle soreness resulting from muscle damage.

    DOI

  • Effects of breaking sitting by standing and acute exercise.

    Takahashi M, Miyashita M, Park JH, Sakamoto S, Suzuki K

    Asian Journal of Sports Medicine   in press ( 3 )  2015  [Refereed]

     View Summary

    Background: Sedentary behavior, which includes sitting and TV viewing, has been identified as an independent risk factor for type 2 diabetes and cardiovascular disease. Breaking sedentary behavior improves metabolic health such as postprandial glycaemia and insulinemia. However, the mechanisms underlying this effect are unclear. Objectives: Here, we examined whether breaking sitting by standing and acute exercise reduces postprandial oxidative stress. Patient and Methods: Fifteen participants performed 3 trials (sitting, standing, and exercise), each lasting 2 days, in a randomised order. On day one of sitting trial, participants sat in a chair. For the standing trial, the participants stood 6 times, for a 45-minute period each time. For the exercise trial, the participants walked or ran at approximately 60% of age-predicted maximum heart rate for 30 minutes. On day two of each trial, participants rested and consumed the standardised breakfast and lunch. Blood samples were collected in the morning and afternoon on day one, and fasting and at 2, 4, and 6 hours postprandially on day two. Results: The concentrations of serum derivatives of reactive oxygen metabolites (d-ROMs) measured at 4 hours (P = 0.064) and 6 hours (P = 0.071) tended to be higher than that in the fasting state in the sitting trial, but not standing and exercise trial (two-factor analysis of variance (ANOVA), trial × time interaction, P = 0.006). Conclusions: Our results indicate the importance of reducing sitting time for improving postprandial oxidative stress status.

    DOI

  • Changes of thioredoxin, oxidative stress markers, inflammation and muscle/renal damage following intensive endurance exercise

    Kaoru Sugama, Katsuhiko Suzuki, Kayo Yoshitani, Koso Shiraishi, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, Takashi Kometani

    EXERCISE IMMUNOLOGY REVIEW   21   130 - 142  2015

     View Summary

    Thioredoxin (TRX) is a 12 kDa protein that is induced by oxidative stress, scavenges reactive oxygen species (ROS) and modulates chemotaxis. Furthermore it is thought to play a protective role in renal ischemia/reperfusion injury Complement 5a (C5a) is a chemotactic factor of neutrophils and is produced after ischemia/reperfusion injury in the kidney. Both TRX and C5a increase after endurance exercise. Therefore, it may be possible that TRX has an association with C5a in renal disorders and/or renal protection caused by endurance exercise. Accordingly, the aim of this study was to investigate relationships among the changes of urine levels of TRX, C5a and acute kidney injury (AKI) caused by ischemia/repetfusion, inflammatory responses, and oxidative stress following intensive endurance exercise. Also, we applied a newly-developed measurement system of neutrophil migratory activity and ROS-production by use of ex vivo hydrogel methodology with an extracellular matrix to investigate the mechanisms of muscle damage. Fourteen male triathletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling and 5 km of running were recruited to the study Venous blood and urine samples were collected before. immediately following, 1.5 It and 3 It after the race. Plasma, serum and urine were analyzed using enzyme-linked immunosorbent assays, a free radical analytical system, and the ex vivo neutrophil functional measurement system. These data were analyzed by assigning participants to damaged and minor-damage groups by the presence and absence of renal tubular epithelial cells in the urinary sediments. We found strong associations among urinary TRX C5a. interleukin (IL)-2, IL-4, IL-8. IL-10, interferon (IFN)-gamma and monocyte chemotactic protein (MCP)-1. From the data it might be inferred that urinary TRX, MCP-1 and beta-N-acetyl-D-glucosaminidase (NAG) were associated with renal tubular injury. Furthermore. TRX may be influenced by levels of IL-10, regulate chemotactic activity of C5a and IL-8, and control inflammatory progress by C5a and In the longer duration group (minor-damage group), circulating neutrophil count, plasma concentration of myeloperoxidase (MPO) and serum concentration of myoglobin were markedly increased. In the higher intensity group (damaged group), neutrophil activation and degranulation of MPO might be inhibited, because not only was ROS production observed to be higher. hut also antioxidant capacity and an inflammatory cytokines were increased. Critically, the newly-developed ex vivo methodology corroborated the neutrophil activation levels in the two groups of participants.

  • Cytokine expression and secretion by skeletal muscle cells: regulatory mechanisms and exercise effects

    Jonathan M. Peake, Paul Della Gatta, Katsuhiko Suzuki, David C. Nieman

    EXERCISE IMMUNOLOGY REVIEW   21   8 - 25  2015

     View Summary

    Cytokines are important mediators of various aspects of health and disease, including appetite, glucose and lipid metabolism, insulin sensitivity skeletal muscle hypertrophy and atrophy Over the past decade or so, considerable attention has focused on the potentiallar regular exercise to counteract a range of disease states by modulating cylokine production. Exercise stimulates moderate to large increases in the circulating concentrations of interleukin IL-8, IL-10, IL-1 receptor antagonist. granulocyte-colony stimulating factor, and smaller increases in tumor necrosis factor-alpha, monocyte chentotactic protein-I, IL-1 beta, brain-derived neurotrophic factor; IL-12p35/p40 and IL-15. Although many of these cytokines are also expressed in skeletal muscle, not all are released from skeletal muscle into the circulation during exercise. Conversely some cytokines that are present in the circulation are not expressed in skeletal muscle after exercise. The reasons for these discrepant cytokine responses to exercise are unclear In this review, we address these uncertainties by summarizing the capacity of skeletal muscle cells to produce cytokines, analyzing other potential cellular sources of circulating cytokines during exercise. and discussing the soluble factors and intracellular signaling pathways that regulate cytokine synthesis (e.g., RNA-binding proteins, micaoRNAs, suppressor of cytokine signaling proteins, soluble receptors).

  • Acute ingestion of catechin-rich green tea improves postprandial glucose status and increases serum thioredoxin concentrations in postmenopausal women

    Masaki Takahashi, Masashi Miyashita, Katsuhiko Suzuki, Seong-ryu Bae, Hyeon-Ki Kim, Takuya Wakisaka, Yuji Matsui, Masao Takeshita, Koichi Yasunaga

    BRITISH JOURNAL OF NUTRITION   112 ( 9 ) 1542 - 1550  2014.11  [Refereed]

     View Summary

    Elevated postprandial hyperglycaemia and oxidative stress increase the risks of type 2 diabetes and CVD. Green tea catechin possesses antidiabetic properties and antioxidant capacity. In the present study, we examined the acute and continuous effects of ingestion of catechin-rich green tea on postprandial hyperglycaemia and oxidative stress in healthy postmenopausal women. Participants were randomly assigned into the placebo (P, n 11) or green tea (GT, n 11) group. The GT group consumed a catechin-rich green tea (catechins 615 mg/350 ml) beverage per d for 4 weeks. The P group consumed a placebo (catechins 92 mg/350 ml) beverage per d for 4 weeks. At baseline and after 4 weeks, participants of each group consumed their designated beverages with breakfast and consumed lunch 3 h after breakfast. Venous blood samples were collected in the fasted state (0 h) and at 2, 4 and 6 h after breakfast. Postprandial glucose concentrations were 3% lower in the GT group than in the P group (three-factor ANOVA, group x time interaction, P&lt;0.05). Serum concentrations of the derivatives of reactive oxygen metabolites increased after meals (P&lt;0.05), but no effect of catechin-rich green tea intake was observed. Conversely, serum postprandial thioredoxin concentrations were 5% higher in the GT group than in the P group (three-factor ANOVA, group x time interaction, P&lt;0.05). These findings indicate that an acute ingestion of catechin-rich green tea has beneficial effects on postprandial glucose and redox homeostasis in postmenopausal women.

    DOI

  • Low-Volume Walking Program Improves Cardiovascular-Related Health in Older Adults

    Jong-Hwan Park, Masashi Miyashita, Masaki Takahashi, Noriaki Kawanishi, Harumi Hayashida, Hyun-Shik Kim, Katsuhiko Suzuki, Yoshio Nakamura

    JOURNAL OF SPORTS SCIENCE AND MEDICINE   13 ( 3 ) 624 - 631  2014.09

     View Summary

    Although numerous sources of evidence show that regular physical activity is beneficial to health, most individuals do not engage in a sufficient amount of physical activity to meet the guidelines set out by expert panels. In addition, the minimum amount of physical activity associated with reduced cardiovascular disease risk markers is not clear in older adults. The purpose of this study was to determine the effects of a 12-week walking program involving an exercise volume below the current minimum physical activity recommendation on cardiovascular disease risk markers in older adults. The participants were recruited from the following two groups separately: a walking group (n = 14) and a control group (n = 14). In the walking group, participants walked 30 to 60 minutes per session on 2 days per week for 12 weeks (average walking time, 49.4 +/- 8.8 min/session). Plasma oxidised low-density lipoprotein concentrations tended to be lower than baseline values in the walking group after 12 weeks (paired t-test, p = 0.127). The ratio of oxidised low-density lipoprotein to high-density lipoprotein cholesterol was significantly lower than the baseline ratio in the walking group after 12 weeks (paired t-test, p = 0.035). Resting systolic blood pressure and diastolic blood pressure were significantly lower than baseline values in the walking group after 12 weeks (paired t-tests, p = 0.002, p < 0.0005, respectively). Our findings demonstrate that a 12-week walking program comprising a low volume of physical activity confers a benefit to cardiovascular-related health in older adults.

  • 運動実施時間帯の違いが一過性持久性運動時における代謝関連指標ならびにホルモン応答に及ぼす影響

    金 鉉基, 高橋将記, 小西真幸, 田端宏樹, 遠藤直哉, 沼尾成晴, 鈴木克彦, 坂本静男

    臨床スポーツ医学   22 ( 3 ) 497 - 505  2014.08

  • Menstrual cycle phase and carbohydrate ingestion alter immune response following endurance exercise and high intensity time trial performance test under hot conditions

    Hideki Hashimoto, Toshimichi Ishijima, Harumi Hayashida, Katsuhiko Suzuki, Mitsuru Higuchi

    JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION   11 ( 11 ) 39  2014.08  [Refereed]

     View Summary

    Background: Sex hormones are known to regulate some responses during exercise. Evaluation of the differences in exercise response with regard to menstrual cycle will help understand the menstrual cycle phase specific adaptations to exercise and athletic performance.Methods: We investigated the effects of menstrual cycle phase and carbohydrate (CHO) ingestion on immune response during endurance exercise at 30 degrees C. Six healthy women completed 4 trials comprising 90 min of cycling at 50% peak aerobic power ((V) over dotO(2peak)) and a high intensity time trial performance test (POST). They ingested a placebo- or CHO-containing beverage during the trials, which were performed during both the follicular and luteal phases of the menstrual cycle. In all trials, thermoregulatory, cardiorespiratory, and immune responses were measured during exercise and after POST.Results: Although the thermoregulatory responses differed between the menstrual cycle phases, the cardiorespiratory responses were not different. After placebo ingestion, leukocyte concentration (cells/mu L) at POST (15.9 x 10(3)) in the luteal phase was significantly higher than that in the follicular phase (12.9 x 10(3)). The rise in leukocyte concentration was attenuated upon CHO ingestion, and the difference between menstrual cycle phases disappeared. A significant positive correlation was found between leukocyte concentration and serum free fatty acid concentrations. Interleukin-6, calprotectin, and myeloperoxidase concentrations significantly increased at POST in all trials, but no significant differences were observed between menstrual cycle phase or beverage type. Concentrations of other cytokines did not change during exercise in any of the 4 trials. Menstrual cycle phase and beverage type had no significant effect on the POST outcome. Thus, differences in leukocyte mobilization between menstrual cycle phases could result from the effect of sex hormones on substrate utilization.Conclusions: The menstrual cycle affected circulating leukocyte concentrations during endurance exercise with POST when a placebo was ingested. Therefore, we recommend ingesting CHO beverages to attenuate immune disturbances, especially in the luteal phase, even though they are unlikely to enhance test performance.

    DOI

  • Effects of Curcumin Supplementation on Exercise-Induced Oxidative Stress in Humans

    M. Takahashi, K. Suzuki, H. K. Kim, Y. Otsuka, A. Imaizumi, M. Miyashita, S. Sakamoto

    INTERNATIONAL JOURNAL OF SPORTS MEDICINE   35 ( 6 ) 469 - 475  2014.06  [Refereed]

     View Summary

    The purpose of this study was to investigate the effects of curcumin supplementation on exercise-induced oxidative stress in humans. 10 male participants, ages 26.8 +/- 2.0 years (mean +/- SE), completed 3 trials in a random order: (1) placebo (control), (2) single (only before exercise) and (3) double (before and immediately after exercise) curcumin supplementation trials. Each participant received oral administration of 90 mg of curcumin or the placebo 2 h before exercise and immediately after exercise. Each participant walked or ran at 65 % of (V) over dotO(2max) on a treadmill for 60 min. Blood samples were collected pre-exercise, immediately after exercise and 2 h after exercise. The concentrations of serum derivatives of reactive oxygen metabolites measured immediately after exercise were significantly higher than pre-exercise values in the placebo trial (308.8 +/- 12.9 U. CARR, P &lt; 0.05), but not in the single (259.9 +/- 17.1 U. CARR) or double (273.6 +/- 19.7 U. CARR) curcumin supplementation trials. Serum biological antioxidant potential concentrations measured immediately after exercise were significantly elevated in the single and double curcumin supplementation trials compared with pre-exercise values (P &lt; 0.05). These findings indicate that curcumin supplementation can attenuate exercise-induced oxidative stress by increasing blood antioxidant capacity.

    DOI

  • Corticosterone accelerates atherosclerosis in the apolipoprotein E-deficient mouse

    Mitsuharu Okutsu, Vitor A. Lira, Kazuhiko Higashida, Jonathan Peake, Mitsuru Higuchi, Katsuhiko Suzuki

    ATHEROSCLEROSIS   232 ( 2 ) 414 - 419  2014.02

     View Summary

    Chronic stress is an important risk factor for atherosclerosis, which is a chief process in the development of cardiovascular disease. Increased circulating levels of corticosterone have been documented in several animal models of chronic stress. However, it remains to be established whether corticosterone is sufficient to exacerbate atherosclerosis. To test this hypothesis, apolipoprotein E (ApoE)-deficient mice were fed a high-fat diet for 13 weeks with exposure to either corticosterone or vehicle in the drinking water (CORT and Con). Corticosterone treatment significantly increased atherosclerotic plaque area at the aortic root. Such exacerbation of atherosclerosis was accompanied by significantly lower levels of circulating white blood cells and serum interleukin-1 beta (IL-1 beta), and significantly elevated serum concentrations of total cholesterol, low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and small dense low-density lipoprotein (sd-LDL) in CORT mice when compared to Con mice. These findings demonstrate that corticosterone is sufficient to exacerbate atherosclerosis in vivo despite its anti-inflammatory properties and that this marked pro-atherogenic phenotype is primarily associated with increased dyslipidaemia. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

    DOI

  • PHYSIOLOGICAL AND LEUKOCYTE SUBSET RESPONSES TO EXERCISE AND COLD EXPOSURE IN COLD-ACCLIMATIZED SKATERS

    K. Kim, K. Suzuki, J. Peake, N. Ahn, K. Ogawa, Ch Hong, S. Kim, Lee, I, J. Park

    BIOLOGY OF SPORT   31 ( 1 ) 39 - 48  2014  [Refereed]

     View Summary

    We investigated physiological responses and changes in circulating immune cells following exercise in cold and thermoneutral conditions. Participants were short track skaters (n=9) who were acclimatized to cold conditions, and inline skaters (n=10) who were not acclimatized. All skaters were young, and skating at a recreational level three days per week for at least one year. Using a cross-over design, study variables were measured during 60 min of submaximal cycling (65% VO(2)max) in cold (ambient temperature: 5 +/- 1 degrees C, relative humidity: 41 +/- 9%) and thermoneutral conditions (ambient temperature: 21 +/- 1 degrees C, relative humidity: 35 +/- 5%). Heart rate, blood lactate and tympanic temperature were measured at rest, during exercise and recovery. Plasma cortisol, calprotectin and circulating blood cell numbers were measured before and after 60 min of cold or thermoneutral conditions, and during recovery from exercise. Heart rate was lower in both groups during exercise in cold versus thermoneutral conditions (P&lt;0.05). The increase in total leukocytes during recovery was primarily due to an increase in neutrophils in both groups. The cold-acclimatized group activated neutrophils after exercise in cold exposure, whereas the non-acclimatized group activated lymphocyte and cortisol after exercise in cold exposure. Lymphocyte subsets significantly changed in both groups over time during recovery as compared to rest. Immediately after exercise in both groups, CD16+ and CD69+ cells were elevated compared to rest or before exercise in both conditions. Acclimatization to exercise in the cold does not appear to influence exercise-induced immune changes in cold conditions, with the possible exception of neutrophils, lymphocytes and cortisol concentration.

    DOI

  • Green Tea Consumption after Intense Taekwondo Training Enhances Salivary Defense Factors and Antibacterial Capacity

    Shiuan-Pey Lin, Chia-Yang Li, Katsuhiko Suzuki, Chen-Kang Chang, Kuei-Ming Chou, Shih-Hua Fang

    PLOS ONE   9 ( 1 ) e87580  2014.01  [Refereed]

     View Summary

    The aim of this study was to investigate the short-term effects of green tea consumption on selected salivary defense proteins, antibacterial capacity and anti-oxidation activity in taekwondo (TKD) athletes, following intensive training. Twenty-two TKD athletes performed a 2-hr TKD training session. After training, participants ingested green tea (T, caffeine 6 mg/kg and catechins 22 mg/kg) or an equal volume of water (W). Saliva samples were collected at three time points: before training (BT-T; BT-W), immediately after training (AT-T; AT-W), and 30 min after drinking green tea or water (Rec-T; Rec-W). Salivary total protein, immunoglobulin A (SIgA), lactoferrin, a-amylase activity, free radical scavenger activity (FRSA) and antibacterial capacity were measured. Salivary total protein, lactoferrin, SIgA concentrations and a-amylase activity increased significantly immediately after intensive TKD training. After tea drinking and 30 min rest, a-amylase activity and the ratio of a-amylase to total protein were significantly higher than before and after training. In addition, salivary antibacterial capacity was not affected by intense training, but green tea consumption after training enhanced salivary antibacterial capacity. Additionally, we observed that salivary FRSA was markedly suppressed immediately after training and quickly returned to pre-exercise values, regardless of which fluid was consumed. Our results show that green tea consumption significantly enhances the activity of a-amylase and salivary antibacterial capacity.

    DOI

  • Effect of aging and sex on circulating microRNAs in humans.

    Sawada S, Akimoto T, Takahashi M, Sakurai R, Shinkai S, Ushida T, Fujiwara Y, Suzuki K

    Adv. Aging Res.   3   152 - 159  2014

  • Exploring the importance of translational regulation in the inflammatory responses by a genome-wide approach

    Hiroaki Sako, Katsuhiko Suzuki

    EXERCISE IMMUNOLOGY REVIEW   20   55 - 67  2014  [Refereed]

     View Summary

    It is widely recognized that exercise has an important role in inflammation regulation. To understand inflammatory mechanisms, extensive studies on the transcriptome and proteome have been conducted. However, interpreting these results is difficult, partly due to technical difficulties that impose some restriction on the accuracy and comprehensiveness of measurements. Here we first mention some limitations of studies involving large scale proteomics and high-throughput transcriptomics and further introduce a newly developed genome-wide translational analysis which may overcome some of the limitations and discover novel cellular dynamics. We then show preliminary results obtained by conducting a genome-wide translational analysis of the early inflammatory response of macrophages in response to lipopolysaccharide (LPS), and discuss the potential to identin) novel factors by employing a genome-wide translational analysis.

  • Evaluation of serum leaking enzymes and investigation into new biomarkers for exercise-induced muscle damage

    Kazue Kanda, Kaoru Sugama, Jun Sakuma, Yasuo Kawakami, Katsuhiko Suzuki

    EXERCISE IMMUNOLOGY REVIEW   20   39 - 54  2014  [Refereed]

     View Summary

    This investigation determined whether existing muscle damage markers and organ damage markers respond to an acute eccentric exercise protocol and are associated with affected muscle symptoms. Nine healthy-young men completed one-leg calf-raise exercise with their right leg on a force plate. They performed 10 sets of 40 repetitions of exercise at 0.5 Hz with a load corresponding to half of their body weight, with 3 mm rest between sets. The tenderness of medial gastrocnemius, lateral gastrocnemius and soleus, and the ankle active range of motion (ROM) were assessed before, immediately after, 24 h and 48 h, 72 h, 96 h and 168 h after exercise. Blood and urine were collected pre-exercise and 2 h, 4 h, 24 h, 48 h, 72 h and 96 h post-exercise. Serum was analyzed for creatine kinase (CK), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and aldolase (ALD) activities. We also determined hearttype fatty acid-binding protein (H-FABP), intestinal-type fatty acid-binding protein (I-FABP) and liver-type fatty acid-binding protein (L-FABP), neutrophil gelatinase-associated lipocalin (NGAL), interleukin (IL)-17A, IL-23, nerve growth factor (NGF), soluble-Endothelial (sE)-selectin, s-Leukocyte (L)-selectin, 5Platelets (P)-selectin, and 8-isoprostane in plasma and urine. The tenderness of proximal and middle gastrocnemius increased significantly 72 h (p&lt;0.05, p&lt;0.01) after exercise. Ankle active ROM in dorsal flexion decreased significantly 48 h (p&lt; 0.05) and 72 h (p&lt;0.01) after exercise. CK and ALD activities significantly increased at 72 h (p&lt;0.05) and remained elevated at 96 h (p&lt; 0.01) postexercise compared to pre-exercise values. Also, ALD which showed relatively lower interindividual variability was significantly correlated with tenderness of middle gastrocnemius at 72 h. LDH activity significantly increased 96 h postexercise (p&lt; 0.01), whereas the increase in AST and ALT activities 96 h post-exercise was not significantly different from pre-exercise values. There were no significant changes in FABPs, NGAL, IL-17A, IL-23, NGF, selectins and 8-isoprostanes in plasma and urine. In conclusion, calf-raise exercise induced severe local muscle damage symptoms which were accompanied by increases in both serum CK and ALD activities, but we could not detect any changes in examined markers of organ damage, inflammation and oxidative stress. Further research is needed to determine other more sensitive biomarkers and the underlying mechanisms of exercise-induced muscle damage.

  • The effect of a sports drink based on highly branched cyclic dextrin on cytokine responses to exhaustive endurance exercise.

    Suzuki K, Shiraishi K, Yoshitani K, Sugama K, Kometani T

    J.Sports Med. Phys.Fitness   54 ( 5 ) 622 - 630  2014

  • Curcumin attenuates oxidative stress following downhill running-induced muscle damage

    Noriaki Kawanishi, Kouki Kato, Masaki Takahashi, Tsubasa Mizokami, Yoshihiko Otsuka, Atsushi Imaizumi, Daisuke Shiva, Hiromi Yano, Katsuhiko Suzuki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   441 ( 3 ) 573 - 578  2013.11  [Refereed]

     View Summary

    Downhill running causes muscle damage, and induces oxidative stress and inflammatory reaction. Recently, it is shown that curcumin possesses anti-oxidant and anti-inflammatory potentials. Interestingly, curcumin reduces inflammatory cytokine concentrations in skeletal muscle after downhill running of mice. However, it is not known whether curcumin affects oxidative stress after downhill running-induced muscle damage. Therefore, the purpose of this study was to investigate the effects of curcumin on oxidative stress following downhill running induced-muscle damage. We also investigated whether curcumin affects macrophage infiltration via chemokines such as MCP-1 and CXCL14. Male C57BL/6 mice were divided into four groups; rest, rest plus curcumin, downhill running, or downhill running plus curcumin. Downhill running mice ran at 22 m/min, -15% grade on the treadmill for 150 min. Curcumin (3 mg) was administered in oral administration immediately after downhill running. Hydrogen peroxide concentration and NADPH-oxidase mRNA expression in the downhill running mice were significantly higher than those in the rest mice, but these variables were significantly attenuated by curcumin administration in downhill running mice. In addition, mRNA expression levels of MCP-1, CXCL14 and F4/80 reflecting presence of macrophages in the downhill running mice were significantly higher than those in the rest mice. However, MCP-1 and F4/80 mRNA expression levels were significantly attenuated by curcumin administration in downhill running mice. Curcumin may attenuate oxidative stress following downhill running-induced muscle damage. (C) 2013 Elsevier Inc. All rights reserved.

    DOI

  • Exercise training attenuates adipose tissue fibrosis in diet-induced obese mice

    Noriaki Kawanishi, Hiroyuki Niihara, Tsubasa Mizokami, Hiromi Yano, Katsuhiko Suzuki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   440 ( 4 ) 774 - 779  2013.11  [Refereed]

     View Summary

    Tissue fibrosis, such as that which occurs in obesity, is associated with chronic inflammatory diseases. Although regular exercise reduces adipose tissue inflammation, the mechanisms regulating the effects of exercise on adipose tissue fibrosis are unclear. This study aimed to clarify whether exercise training attenuates adipose tissue fibrosis with consequent reduction of extracellular matrix including collagens. Male C57BL/6J (4-week old) mice were randomly assigned to four groups that received a normal diet (ND) plus sedentary (n = 8), an ND plus exercise training (n = 8), a high-fat diet (HFD) plus sedentary (n = 12), and an HFD plus exercise training (n = 12). Mice were fed the ND or HFD from 4 to 20 weeks of age. The exercise groups were trained on a motorized treadmill for 60 min/day, 5 times/week over the same period. Histological hepatic fibrosis detected by Sirius red and alpha-smooth muscle actin staining were attenuated in HFD exercise mice compared with HFD sedentary mice. mRNA levels of transforming growth factor-beta and tissue inhibitors of metalloproteinase-1, major regulators of tissue fibrosis, were increased in HFD sedentary mice but were attenuated in HFD exercise mice. Similarly, adipose tissue from the HFD sedentary mice contained higher macrophages than adipose tissue from the ND mice, and this was also lowered by exercise training. These findings suggest that exercise training may be effective for attenuating adipose tissue inflammation in obesity. (C) 2013 Elsevier Inc. All rights reserved.

    DOI

  • A pilot study: Bovine colostrum supplementation and hormonal and autonomic responses to competitive cycling

    C. M. Shing, J. M. Peake, K. Suzuki, D. G. Jenkins, J. S. Coombes

    Journal of Sports Medicine and Physical Fitness   53 ( 5 ) 490 - 501  2013.10

     View Summary

    Aim. The aim of this pilot investigation was to examine the influence of bovine colostrum protein concentrate (CPC) supplementation on salivary hormones, salivary IgA and heart rate variability over consecutive days of competitive cycling. Methods. Ten highly-trained male road cyclists (mean±SEM
    age=22.2±4.7 yr
    mass=70.5±4.5 kg
    VO2max=72. 9±3.8 mL.kg-1.min-1) were randomly assigned to a control (N.=6, 10g whey protein concentrate/day) or bovine CPC group (N.=4, 10 g bovine CPC/day). Cyclists provided a baseline saliva sample before commencing eight weeks of supplementation, and competing in a five day cycle race. Cyclists provided saliva samples and measured heart rate variability (HRV) each day of the race. Saliva samples were analysed for cortisol, testosterone and IgA concentrations. Results. Bovine CPC supplementation was associated with increased morning cortisol concentration on the first day of racing when compared to the control group (P=0.004) and significantly prevented a decrease in testosterone concentration over the race period (P≤0.05). Across the race period parasympathetic indices of HRV were elevated in the bovine CPC group and reduced in the control group (P≤0.05), while there were no significant differences in salivary IgA between groups. Conclusion. Bovine CPC supplementation maintained salivary testosterone concentration and modulated autonomic activity over consecutive days of competitive cycling. This pilot study provides justification to explore the effects of bovine CPC on recovery in endurance athletes further.

    PubMed

  • The association between physical activity and sex-specific oxidative stress in older adults.

    Takahashi M, Miyashita M, Park JH, Kim HS, Nakamura Y, Sakamoto S, Suzuki K

    J. Sports Sci. Med.   12 ( 3 ) 571 - 578  2013.09

     View Summary

    Oxidative stress increases with advancing age and is a mediator of several diseases including cancer, cardiovascular disease, and diabetes. Moreover, postmenopausal women have a lower estrogen concentration, which is associated with elevated oxidative stress. However, there is no definitive evidence regarding the relationship between daily physical activity and oxidative stress status in older adults, including postmenopausal women. Twenty- nine adults (age, 70.1 ± 1.0 years, mean ± SE; 12 women and 17 men) were examined in this cross-sectional study. Prior to blood collection, the participants were asked to wear a uniaxial accelerometer for 4 consecutive weeks to determine their level of physical activity. After a 48-h period of physical activity avoidance and a 10-h overnight fast, venous blood samples were obtained from each participant. Fasting plasma derivatives of reactive oxygen metabolites (d-ROMs) and malondialdehyde (MDA) concentrations of oxidative stress markers were negatively correlated with the amount of physical activity in women (d-ROMs; r = -0.708, p = 0.002) (MDA; r = -0.549, p = 0.028), but not in men. Fasting plasma biological antioxidant potential of antioxidant capacity marker was positively correlated with the amount of physical activity in women (BAP; r = 0.657, p = 0.006) (GSH; r = 0.549, p = 0.028), but not in men. Moreover, superoxide dismutase activity of antioxidant capacity marker was positively correlated with the amount of physical activity in men (r = 0.627, p = 0.039), but not in women. There were no associations between physical activity and other oxidative stress markers (reduced and oxidized glutathione, glutathione peroxidise, thioredoxin). These findings suggest that regular physical activity may have a protective effect against oxidative stress by increasing total antioxidant capacity, especially in postmenopausal women. © Journal of Sports Science and Medicine.

  • The Effects of Sports Drink Osmolality on Fluid Intake and Immunoendocrine Responses to Cycling in Hot Conditions

    Katsuhiko Suzuki, Hideki Hashimoto, Taewoong Oh, Toshimichi Ishijima, Hiromichi Mitsuda, Jonathan M. Peake, Shizuo Sakamoto, Isao Muraoka, Mitsuru Higuchi

    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY   59 ( 3 ) 206 - 212  2013.06

     View Summary

    We investigated the effects of two carbohydrate-based sports drinks on fluid intake and immunoendocrine responses to cycling. Six well-trained male cyclists completed trials on three separate days that involved cycling at 60% V(O) over dot(2peak) for 90 min in hot conditions (28.1 +/- 1.5 degrees C and 52.6 +/- 3.1% relative humidity). During each trial, the subjects consumed ad libitum (1) an isotonic sports drink (osmolality 317 mOsm/kg), (2) a hypotonic sports drink (osmolality 193 mOsm/kg) or (3) plain water. The cyclists consumed significantly (p&lt;0.05) more of the isotonic drink (1.23 +/- 0.35 L) and hypotonic drink (1.44 +/- 0.55 L) compared with water (0.73 +/- 0.26 L). Compared with water ( 0.96 +/- 0.26 kg), body mass decreased significantly less after consuming the hypotonic drink (-0.50 +/- 0.38 kg) but not the isotonic drink (-0.51 +/- 0.41 kg). Blood glucose concentration was significantly higher at the end of the isotonic and hypotonic drink trials compared with the water trial. Neutrophil count and the plasma concentrations of catecholamines, interleukin 6 (IL-6), myeloperoxidase, calprotectin and myoglobin increased significantly during all three trials. IL-6 and calprotectin were significantly lower following the hypotonic drink trial compared with the water trial. In conclusion, hypotonic sports drinks are appealing for athletes to drink during exercise, and may help to offset fluid losses and attenuate some inflammatory responses to exercise.

  • Effects of low-volume walking programme and vitamin E supplementation on oxidative damage and health-related variables in healthy older adults.

    Park JH, Miyashita M, Takahashi M, Kawanishi N, Bae SR, Kim HS, Suzuki K, Nakamura Y

    Nutrition & metabolism   10 ( 1 ) 38  2013.05  [Refereed]

     View Summary

    Background: Both exercise and vitamin E supplementation have been shown to reduce oxidative stress and cardiovascular disease risk in older adults, and when combined there is evidence suggesting that they act synergistically. The currently recommended amount of exercise for older adults is 150 min/week of moderate-intensity exercise; however, the minimum amount of exercise necessary to achieve health benefits is not known. The purpose of this study was to investigate the effects of 12 weeks of participation in a low-volume walking exercise programme (i.e. 90 min/week) combined with daily vitamin E supplementation on thiobarbituric acid reactive substances (TBARS) and oxidised low-density lipoprotein (LDL) concentrations in older adults. Methods. The participants were recruited from the following four groups separately: 1) control (CG, n = 14), 2) vitamin E supplementation (SG, n = 10), 3) walking (WG, n = 7), or 4) walking + supplementation (WSG, n = 7). In the CG, participants were advised to maintain their normal lifestyle during the study. Participants in both the SG and WSG received 450 IU (300 mg) /day of α-tocopherol for 12 weeks. The exercise programme for the WG and WSG consisted of two 30-60 minute sessions weekly for 12 weeks (average walking time was 44.5 ± 1.6 min/session). Blood samples were collected at baseline and at 12 weeks. Results: Delta plasma oxidised LDL concentrations did not differ among four groups (One-factor ANOVA, P = 0.116). However, negative delta plasma TBARS, a marker of oxidative damage, concentrations were observed in the WG, WSG and SG relative to the CG at the end of the study period (One-factor ANOVA, P = 0.001; post hoc tests; CG compared with WG, WSG and SG, P = 0.005; P = 0.021; P = 0.024, respectively). Conclusion: These findings suggest that a low-volume of physical activity and/or vitamin E supplementation may be an effective intervention strategy for reducing TBARS concentrations of older adults. Trial registration. UMIN000008304. © 2013 Park et al.; licensee BioMed Central Ltd.

    DOI PubMed

  • Low-volume exercise training attenuate oxidative stress and neutrophils activation in older adults

    Masaki Takahashi, Masashi Miyashita, Noriaki Kawanishi, Jong-Hwan Park, Harumi Hayashida, Huan-Shik Kim, Yoshio Nakamura, Shizuo Sakamoto, Katsuhiko Suzuki

    Eur J Appl Physiol   113 ( 5 ) 1117 - 1126  2013.05  [Refereed]

     View Summary

    The purpose of this study was to investigate the effects of low-volume exercise training (100 min/week) on oxidative stress and neutrophils activation markers in older adults. Twenty-eight older adults (age range 65-78 years) were assigned into control (n = 14) or exercise (n = 14) groups. The exercise program consisted of walking 30-60 min/session, 2 days each week for 12 weeks. Blood samples were taken before starting the sessions (baseline) and when they ended. Fasting plasma and serum oxidative stress and inflammatory markers were measured using commercial kits. Cell surface expression of adhesion molecules on circulating leukocytes (CD66b and CD62L) was determined using flow cytometry. The concentrations of derivatives of reactive oxygen metabolites tended to be lower than the baseline values only in the exercise group (P = 0.05). The biological antioxidant potential, thioredoxin concentrations, and glutathione peroxidase activities significantly increased only in the exercise group (P < 0.05 for all). While CD66b expression tended to decrease only in the exercise group, CD62L expression significantly increased (P < 0.05). Our findings indicate that exercise training below the current recommended level of at least 150 min/week attenuates basal oxidative stress and neutrophil activation in older adults. Thus, our findings may encourage more people to incorporate a small amount of physical activity into their lives. © 2012 Springer-Verlag Berlin Heidelberg.

    DOI PubMed

  • 新規好中球機能検査法によるオリゴノールの機能解析

    鈴木克彦, 泊美樹, 菅間薫, 北舘健太郎, 三浦茂樹, 吉岡浩, 森有一

    日本補完代替医療学会誌   10 ( 1 ) 59 - 62  2013.03

  • リボソームプロファイリングのバイオマーカー開発への応用

    佐古博皓, 鈴木克彦, 竹山春子

    日本補完代替医療学会誌   10 ( 1 ) 1 - 7  2013.03

  • Effects of one night of sleep deprivation on maximal fat oxidation during graded exercise.

    Konishi M, Takahashi M, Endo N, Numao S, Takagi S, Miyashita M, Midorikawa T, Suzuki K, Sakamoto S

    The Journal of Physical Fitness and Sports Medicine   2 ( 1 ) 121 - 126  2013  [Refereed]

  • Effects of low-volume physical activity and vitamin E supplementation on thiobarbituric acid reactive substances and oxidised low-density lipoprotein concentrations in older adults.

    Park JH, Miyashita M, Takahashi M, Kawanishi N, Bae S, Kim H, Suzuki K, Nakamura Y

    Nutr. Metab (Lond)   10   38  2013

  • Effects of a comprehensive intervention program, including hot bathing, on overweight adults: A randomized controlled trial.

    Sakurai R, Fujiwara Y, Saito K, Fukaya T, Kim MJ, Yasunaga M, Kim H, Ogawa K, Tanaka C, Tsunoda N, Muraki E, Suzuki K, Shinkai S, Watanabe S

    Geriatr Gerontol Int 2012 Oct 24.   1 ( 3 ) 638 - 645  2013

  • Profiling of circulating microRNAs after a bout of acute resistance exercise in humans.

    Sawada S, Kon M, Wada S, Ushida T, Suzuki K, Akimoto T

    PLOS ONE   8 ( 7 ) e70823  2013

  • Mechanisms of chronic inflammation improvement by exercise: Focus on immune response of local tissue.

    Kawanishi N, Yano H, Mizokami T, Suzuki K

    J. Phys. Fitness Sports Med.   2   487 - 492  2013

  • Effects of sleep deprivation on autonomic and endocrine functions in sleep deprivation throughout the day and on exercise tolerance in the evening.

    Konishi M, Takahashi M, Endo N, Numao S, Takagi S, Miyashita M, Midorikawa T, Suzuki K, Sakamoto S

    J Sports Sci   31 ( 3 ) 248 - 255  2013

  • Postprandial Lipaemia: Effects of sitting, standing and walking in healthy normolipidaemic humans.

    Miyashita M, Park JH, Takahashi M, Suzuki K, Stensel DJ, Nakamura Y

    Int J Sports Med   34 ( 1 ) 21 - 27  2013

  • Takahashi M, Miyashita M, Kawanishi N, Park JH, Hayashida H, Kim HS, Nakamura Y, Sakamoto S, Suzuki K.

    Low-volume exercise training, attenuates oxidative stress, neutrophils activation in, older adults

    Eur. J. Appl. Physiol.   113   1117 - 1126  2013

  • Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation.

    Kazue Kanda, Kaoru Sugama, Harumi Hayashida, Jun Sakuma, Yasuo Kawakami, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, Katsuhiko Suzuki

    Exercise immunology review   19   72 - 85  2013  [International journal]

     View Summary

    The purpose of this study was to determine the relationships among delayed-onset muscle soreness (DOMS), muscle damage and inflammatory responses to eccentric exercise and investigate the underlying mechanisms. Nine healthy males performed one-leg calf-raise exercise with their right leg on a force plate. They performed 10 sets of 40 repetitions of exercise at 0.5 Hz by the load corresponding to the half of their body weight, with a rest for 3 min between sets. DOMS was evaluated by a visual analogue scale (VAS). Blood and urine samples were collected before and 2, 4, 24, 48, 72 and 96 h post-exercise. Blood samples were analyzed for leucocyte differential counts and neutrophil functions (migratory activity and oxidative burst activity). We also determined a serum marker of muscle damage, myoglobin (Mb), and plasma and urinary prostaglandin E2 as an algesic substance. As for the inflammatory mediators, plasma and urine were analyzed for cytokines (interleukin (IL)-1beta, IL-1 receptor antagonist, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p40, IL-12p70, tumour necrosis factor-alpha, interferon-gamma, monocyte chemotactic protein-1, granulocyte colony-stimulating factor, macrophage colony-stimulating factor, and granulocyte macrophage colony-stimulating factor), leucocyte activation markers (calprotectin and myeloperoxidase), and neutrophil chemotactic factor complement 5a. All subjects reported muscle soreness on subsequent days and VAS peaked at 72 h after exercise. Serum Mb concentration significantly increased (p < 0.05) at 72 h after exercise as compared with the pre-exercise values which was correlated with the increases in VAS at 72 h (r = 0.73, p < 0.05). Circulating neutrophil count and migratory activity increased significantly (p < 0.01, and p < 0.05, respectively) at 4 h after exercise, whereas there were no significant changes in the other plasma and urinary inflammatory mediators. These results suggest that neutrophils can be mobilized into the circulation and migrate to the muscle tissue several hours after the eccentric exercise. There were also positive correlations between the exercise-induced increases in neutrophil migratory activity at 4 h and the increases in Mb at 48 h (r = 0.67, p < 0.05). These findings suggest that neutrophil mobilization and migration after exercise may be involved in the muscle damage and inflammatory processes.

    PubMed

  • Urinary excretion of cytokines versus their plasma levels after endurance exercise

    Kaoru Sugama, Katsuhiko Suzuki, Kayo Yoshitani, Koso Shiraishi, Takashi Kometani

    EXERCISE IMMUNOLOGY REVIEW   19   29 - 48  2013  [Refereed]

     View Summary

    It has been consistently shown that circulating levels of interleukin (IL)-6, IL-8, IL-1 receptor antagonist (IL-1ra) and IL-10 increase remarkably following endurance exercise longer than 2 h such as marathon and triathlon races. However, no studies have compared changes in the plasma and urinary levels of these cytokines after endurance exercise, including the recovery period. In the present study, we investigated kinetic changes in the urinary excretion of cytokines following endurance exercise up to 3 h after exercise to evaluate the magnitude of change in comparison to the plasma levels and to explore the possible biological significance and the mechanisms of cytokine dynamics following exercise. Fourteen male athletes participated in a duathlon race consisting of 5 km of running, 40 km of cycling, and 5 km of running. Venous blood and urine samples were collected before, immediately after, 1.5 h and 3 h after the race. Plasma and urine were analyzed using enzyme-linked immunosorbent assays (ELISA). Plasma concentrations of IL-1 beta, IL-1ra, IL-6, IL-8, IL-10 and monocyte chemotactic protein (MCP)-1 increased significantly after the race, whereas tumour necrosis factor (TNF)-alpha, IL-2, IL-4, IL-12 and interferon (IFN)-gamma did not change significantly. Urinary concentrations of IL-1 beta, IL-1ra, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-gamma and MCP-1 increased significantly after the race. When the urine concentrations were adjusted by creatinine concentration, urine volume and sampling time, the increases of IL-2, IL-4, IL-8, IL-10, IFN-gamma and MCP-1 were evident and these were notably present in urine of the stressed athletes suffering from renal tubular epithelial damage. The present study provides new evidence that the kinetics and magnitude of changes in urinary cytokine concentrations differ from plasma cytokine concentrations following endurance exercise, especially, in the recovery period several hours after exercise, and that the damaged kidney might be responsible at least in part for the kinetics of some cytokines. Urinary cytokines may be sensitive biomarkers of the impact of exhaustive exercise workload on renal damage and inflammation in the recovery period after endurance exercise.

  • Exercise attenuates M1 macrophages and CD8+ T cells in adipose tissue of obese mice.

    Kawanishi N, Mizokami T, Yano H, Suzuki K

    Med. Sci. Sports Exer. (MSSE)   45 ( 9 ) 1684 - 1693  2013

  • 急性完全断眠の糖質・脂質代謝および漸増運動中の最大脂質酸化量に及ぼす影響

    小西 真幸, 高橋 将記, 遠藤 直哉, 沼尾 成晴, 高木 俊, 宮下 政司, 緑川 泰史, 鈴木 克彦, 坂本 静男

    体力科学   61 ( 6 ) 633 - 633  2012.12

  • 新規好中球機能検査法によるクルクミンの抗酸化・抗炎症作用の評価

    鈴木克彦, 泊美樹, 菅間薫, 高橋将記, 三浦茂樹, 吉岡浩, 森有一

    臨床化学   41 ( 4 ) 353 - 358  2012.10

  • 運動ストレスに対する新規バイオマーカーとしての血中microRNA

    鈴木克彦, 秋本崇之

    日本補完代替医療学会誌   9 ( 2 ) 69 - 74  2012.09

  • 新規好中球機能検査法を応用した植物抽出物の機能性評価

    鈴木克彦, 駒場優太, 泊美樹, 鈴木洋子, 菅間薫, 高橋将記, 三浦茂樹, 吉岡浩, 森有一

    日本補完代替医療学会誌   9 ( 2 ) 89 - 95  2012.09

  • Exercise training attenuates hepatic inflammation, fibrosis and macrophage infiltration during diet induced-obesity in mice.

    Kawanishi N, Yano H, Mizokami T, Takahashi M, Oyanagi E, Suzuki K

    Brain, Behavior, and Immunity (BBI)   26 ( 6 ) 931 - 941  2012.08

  • Effects of Different Intensities of Endurance Exercise on Oxidative Stress and Leukocyte Activation Markers

    Masaki Takahashi, Katsuhiko Suzuki, Hideki Matoba, Shizuo Sakamoto, Shigeru Obara

    JOURNAL OF GENERAL INTERNAL MEDICINE   27   382 - 382  2012.07

  • Suzuki K, Ohno S, Suzuki Y, Ohno Y, Okuyama R, Aruga A, Yamamoto M, Ishihara K, Nozaki T, Miura S, Yoshioka H, Mori Y.

    Effect of green, tea extract on, reactive oxygen, species, produced by neutrophils, from cancer patients

    Anticancer Res   32   2369 - 2376  2012.06

  • 人工血小板が炎症・免疫系に及ぼす影響からみた安全性評価に関する研究.

    鈴木克彦

    平成21-23年度厚生労働科学研究費補助金(政策創薬総合研究事業) H12(ADP)リポソームの人工血小板としての前臨床評価(効力と安全性)(総合)研究報告書.     120 - 126  2012.03

  • 血小板減少ウサギモデルにおける外傷性出血に対する人工血小板投与が好中球機能に及ぼす影響.

    鈴木克彦ほか

    平成23年度厚生労働科学研究費補助金(政策創薬総合研究事業) H12(ADP)リポソームの人工血小板としての前臨床評価(効力と安全性)(分担)研究報告書:その2.     71 - 75  2012.03

  • 人工血小板投与が血液生化学検査指標と肝臓の炎症性サイトカイン産生に及ぼす影響.

    鈴木克彦ほか

    平成23年度厚生労働科学研究費補助金(政策創薬総合研究事業) H12(ADP)リポソームの人工血小板としての前臨床評価(効力と安全性)(分担)研究報告書:その1.     67 - 70  2012.03

  • Determination of Chronic Inflammatory States in Cancer Patients Using Assay of Reactive Oxygen Species Production by Neutrophils

    Yoko Suzuki, Satoshi Ohno, Ryuji Okuyama, Atsushi Aruga, Masakazu Yamamo, Shigeki Miura, Hiroshi Yoshioka, Yuichi Mori, Katsuhiko Suzuki

    ANTICANCER RESEARCH   32 ( 2 ) 565 - 570  2012.02  [Refereed]

     View Summary

    Background: Although the systemic inflammatory condition can be confirmed in cancer patients, the pathophysiological importance of reactive oxygen species (ROS) produced by neutrophils has not yet been defined. Patients and Methods: Twenty-one patients with inoperable, chemoresistant and radioresistant cancer were enrolled in this Study. At least 4 weeks prior to sampling, the patients were free from antitumor treatments. Control samples were also obtained from a healthy donor (39-year-old male). Peripheral blood samples were set 150 mu l each on the 2 ml tube with 50 mu l Mebiol Gel, and the production of ROS from neutrophils was detected by luminol-dependent chemiluminescence (LmCL) in a kinetic mode at 30-minute intervals for 2.5 hours with a luminometer at 37 degrees C. Results: Each point, peak value and sum of values of LmCL in the patient group was statistically higher than those in the healthy donor. There were no differences in LmCL according to performance status (PS), type of cancer, age, or gender in cancer patients. Conclusion: Our findings suggested that ROS produced by neutrophils universally reflects the systemic inflammatory condition in cancer patients.

  • IL-17, neutrophil activation and muscle damage following endurance exercise.

    Sugama K, Suzuki K, Yoshitani K, Shiraishi K, Kometani T

    Exercise immunology review   18   116 - 127  2012  [Refereed]

    PubMed

  • Effects of different intensities of endurance exercise on oxidative stress and antioxidant capacity.

    Masaki Takahashi, Katsuhiko Suzuki, Hideki Matoba, Shizuo Sakamoto, Shigeru Obara

    The Journal of Physical Fitness and Sports Medicine   1 ( 1 ) 183 - 189  2012  [Refereed]

    DOI

  • [A cross-sectional study of sarcopenia in Japanese men and women: reference values and association with cardiovascular risk factors and metabolic syndrome].

    Sanada K, Iemitsu M, Tabata I, Miyachi M, Murakami H, Yamamoto K, Hanawa S, Kawakami R, Kawano H, Gando Y, Suzuki K, Higuchi M, Tanimoto M, Omori Y

    Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics   49 ( 6 ) 715 - 717  2012  [Refereed]

    DOI PubMed

  • The effects of attention retraining on depressive mood and cortisol responses to depression-related stimuli. (original paper)

    Tsumura H, Shimada H, Nomura K, Sugaya N, Suzuki K

    Japanese Psychological Research   54 ( 4 ) 400 - 411  2012

  • Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans

    Masashi Morifuji, Tomoko Aoyama, Ai Nakata, Chiaki Sambongi, Jinichiro Koga, Kunihiko Kurihara, Minoru Kanegae, Katsuhiko Suzuki, Mitsuru Higuchi

    EUROPEAN JOURNAL OF SPORT SCIENCE   12 ( 2 ) 152 - 160  2012  [Refereed]

     View Summary

    The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.

    DOI

  • IL-17, neutrophil activation and muscle damage following endurance exercise. (original paper)

    Sugama K, Suzuki K, Yoshitani K, Shiraishi K, Kometani T

    Exerc Immunol Rev   18   115 - 126  2012

  • Translational suppression of atrophic regulators by microRNA-23a integrates resistance to skeletal muscle atrophy.

    Shogo Wada, Yoshio Kato, Mitsuharu Okutsu, Shigeru Miyaki, Katsuhiko Suzuki, Zhen Yan, Stefano Schiaffino, Hiroshi Asahara, Takashi Ushida, Takayuki Akimoto

    The Journal of biological chemistry   286 ( 44 ) 38456 - 65  2011.11  [International journal]

     View Summary

    Muscle atrophy is caused by accelerated protein degradation and occurs in many pathological states. Two muscle-specific ubiquitin ligases, MAFbx/atrogin-1 and muscle RING-finger 1 (MuRF1), are prominently induced during muscle atrophy and mediate atrophy-associated protein degradation. Blocking the expression of these two ubiquitin ligases provides protection against muscle atrophy. Here we report that miR-23a suppresses the translation of both MAFbx/atrogin-1 and MuRF1 in a 3'-UTR-dependent manner. Ectopic expression of miR-23a is sufficient to protect muscles from atrophy in vitro and in vivo. Furthermore, miR-23a transgenic mice showed resistance against glucocorticoid-induced skeletal muscle atrophy. These data suggest that suppression of multiple regulators by a single miRNA can have significant consequences in adult tissues.

    DOI PubMed

  • 断眠の交感神経副腎髄質系およびHPA系に及ぼす影響 唾液中ストレス指標による検討

    小西 真幸, 高橋 将記, 遠藤 直哉, 沼尾 成晴, 高木 俊, 宮下 政司, 緑川 泰史, 鈴木 克彦, 坂本 静男

    日本臨床生理学会雑誌   41 ( 3 ) 129 - 135  2011.06

  • Virus Activation and Immune Function During Intense Training in Rugby Football Players

    R. Yamauchi, K. Shimizu, F. Kimura, M. Takemura, K. Suzuki, T. Akama, I. Kono, T. Akimoto

    INTERNATIONAL JOURNAL OF SPORTS MEDICINE   32 ( 5 ) 393 - 398  2011.05  [Refereed]

     View Summary

    Epidemiological studies suggest that highly trained athletes are more susceptible to upper respiratory tract infections (URTI) compared with the general population. Upper respiratory symptoms (URS) often appear as either primary invasion of pathogenic organisms and/or reactivation of latent viruses such as Epstein-Barr virus (EBV). The purpose of this study was to examine the relationship between EBV reactivation and the appearance of URS during intensive training in collegiate rugby football players. We evaluated EBV-DNA expression in saliva and examined the relationship between onset of URS and daily changes in EBV-DNA as well as secretory immunoglobulin A (SIgA) levels among 32 male collegiate rugby football players during a 1-month training camp. The EBV-DNA expression tended to be higher in subjects who exhibited sore throat (p = 0.07) and cough (p = 0.18) than that of those who had no symptoms, although their differences were not significant. The SIgA level was significantly lower 1 day before the EBV-DNA expression (p &lt; 0.05). The number of URS increased along with the EBV-DNA expression and decrease of SIgA levels. These results suggest that the appearance of URS is associated with reactivation of EBV and reduction of SIgA during training.

    DOI

  • 好中球機能を用いた血小板減少・外傷性出血における人工血小板投与のin vivo評価.

    鈴木克彦ほか

    平成22年度厚生労働科学研究費補助金(創薬基盤推進研究事業:政策創薬総合研究事業) H12(ADP)リポソームの人工血小板としての前臨床評価(効力と安全性)分担研究報告書     54 - 59  2011.03

  • Oxidised low-density lipoprotein concentrations and physical activity status in older adults: the WASEDA active life study.

    Park Jong-Hwan, Miyashita Masashi, Takahashi Masaki, Harada Kazuhiro, Takaizumi Kanae, Kim Hyun-Shik, Suzuki Katsuhiko, Nakamura Yoshio

    Journal of atherosclerosis and thrombosis   18 ( 7 )  2011

     View Summary

    AIM:Oxidised low-density lipoprotein (LDL) is considered a risk marker for cardiovascular disease. However, it remains unclear whether oxidised LDL concentrations differ with the physical activity status in older adults. The purpose of this study was to examine the relationship between the amount of physical activity and oxidised LDL in older adults.;METHODS:Twenty-seven older adults (aged 69.9 ± 4.3 years, mean ± SD.; 16 female and 11 male) were analysed in the cross-sectional design. Prior to blood collection, participants were asked to wear an uniaxial accelerometer for 4 consecutive weeks to determine the physical activity status. After a 48-h period of physical activity avoidance and a 10-h overnight fast, fasting venous blood samples were obtained from each participant.;RESULTS:Fasting plasma oxidised LDL concentrations and plasma monocyte chemoattractant pro-tein-1 concentrations were negatively correlated with the amount of physical activity (r=-0.409, p=0.034; r=-0.385, p=0.047, respectively).;CONCLUSIONS:This study demonstrates that regular physical activity may play a protective role in the oxidation of LDL in older adults.

    PubMed

  • 動脈硬化進展予防に対する身体活動の役割:身体活動と新規動脈硬化症予測マーカーとの関連

    宮下政司, 朴鍾煥, 高橋将記, 鈴木克彦, 中村好男

    財団法人健康管理事業団 研究助成論文集   27   39 - 47  2011

  • 温泉施設を用いた複合的介入プログラムの有効性に関する研究ー無作為化介入試験による検討ー

    桜井良太, 藤原佳典ほ

    日老医誌   48   352 - 360  2011

  • 中等度強度以下の持久性運動が血中の活性酸素生成および白血球活性化マーカーに及ぼす影響(原著論文)

    高橋将記, 鈴木克彦, 的場秀樹, 佐竹昌之, 坂本静男, 小原繁

    日本補完代替医療学会誌   8   25 - 28  2011

  • 運動による自覚的疲労度とストレスホルモン・サイトカインとの関連性(原著論文)

    石渡智子, 森藤雅史, 石島寿道, 青山友子, 菅間薫, 神田和江, 鈴木克彦, 樋口満

    日本補完代替医療学会誌   8   67 - 73  2011

  • Oxidised low-density lipoprotein concentrations and physical activity status in older adults: the WASEDA active life study. (original paper)

    Park JH, Miyashita M, Takahashi M, Harada K, Takaizumi K, Kim HS, Suzuki K, Nakamura Y

    J Atheroscler ThrombPark   8   568 - 573  2011

  • Physical activity status and postprandial lipaemia in older adults. (original paper)

    Miyashita M, Park JH, Takahashi M, Burns S, Kim HS, Suzuki K, Nakamura Y

    Int J Sports Med   32   829 - 834  2011

  • Wada S, Kato Y, Okutsu M, Miyaki S, Suzuki K, Yan Z, Schiaffino S, Asahara H, Ushida T, Akimoto T.

    Translational suppression of atrophic regulators by miR, a, integrates resistance to skeletal muscle atrophy. (original pap

    Journal of Biological Chemistry (JBC)   286   38456 - 38465  2011

  • Resistance exercise training-induced muscle hypertrophy was associated with reduction of inflammatory markers in elderly women. (original paper)

    Ogawa K, Sanada K, Machida S, Okutsu M, Suzuki K

    Mediators of Inflammation   171023  2010.12

  • 月経周期と持久性運動による唾液の酸化ストレス指標の変動(原著論文)

    林田はるみ, 志村まゆら, 菅間薫, 神田和江, 鈴木克彦

    日本補完代替医療学会誌   7 ( 2 ) 125 - 128  2010.09

  • 運動による免疫変動のメカニズムと対応策(解説)

    鈴木克彦

    健康づくり   387  2010.07

  • 運動によるさまざまな免疫変動と意義(解説)

    鈴木克彦

    健康づくり   386  2010.06

  • 運動と感染・炎症・アレルギー疾患(解説)

    鈴木克彦

    健康づくり   385   17  2010.05

  • 免疫とは何か(解説)

    鈴木克彦

    健康づくり   384   17  2010.04

  • 好中球機能・サイトカイン産生能からみた各種人工血小板素材のin vitro評価.

    鈴木克彦ほか

    平成21年度厚生労働科学研究費補助金(創薬基盤推進研究事業) H12(ADP)リポソームの人工血小板としての前臨床評価(効力と安全性)分担研究報告書     54 - 59  2010.04

  • Recovery following an Ironman triathlon: A case study

    Kazunori Nosaka, Chris R. Abbiss, Greig Watson, Bradley Wall, Katushiko Suzuki, Paul Laursen

    EUROPEAN JOURNAL OF SPORT SCIENCE   10 ( 3 ) 159 - 165  2010  [Refereed]

     View Summary

    Completion of an Ironman triathlon results in muscle damage, indicated by reductions in muscle function and muscle soreness. However, the time course of recovery from this damage has received little attention. The purpose of this case study was to examine the time course of changes in blood markers of muscle damage and inflammation, muscle function, muscle soreness, and economy of motion following an Ironman event. An experienced well-trained male triathlete aged 35 years completed the Western Australian Ironman triathlon in 11 h 38 min 41 s (winner's time: 8 h 3 min 56 s). Before and on several occasions in the 15 days after the event, the participant performed an incremental cycling test to exhaustion, running economy test at 12 km center dot h-1 (2% incline), maximal isometric knee flexion and extension at 90 degrees knee flexion, and maximal squat and countermovement jumps. Venous blood samples and muscle soreness were also assessed. Maximal oxygen consumption, efficiency of motion, maximal muscle strength, and jump performance were all markedly reduced (4.5-54%) following the event, but returned to baseline within 15, 8, 2, and 8 days following the event, respectively. Muscle soreness and blood markers peaked 2-24 h after the race but returned to baseline within 8 days. In conclusion, although the Ironman triathlon induces marked muscle damage, a trained triathlete recovered almost completely within approximately one week, without the use of any therapeutic interventions after the event.

    DOI

  • A cross-sectional study of sarcopenia in Japanese men and women: reference values and association with cardiovascular risk factors. (original paper)

    Sanada K, Miyachi M, Tanimoto M, Yamamoto K, Murakami H, Okumura S, Gando Y, Suzuki K, Tabata I, Higuchi M

    Eur J Appl Physiol   110   57 - 65  2010

  • PPARγ2 C1431T genotype increases metabolic syndrome risk in young men with low cardiorespiratory fitness. (original paper)

    Sanada K, Iemitsu M, Murakami H, Tabata I, Yamamoto K, Gando Y, Suzuki K, Higuchi M, Miyachi M

    Physiol Genomics   43   103 - 109  2010

  • Exercise training inhibits inflammation in adipose tissue via both suppression of macrophage infiltration and acceleration of phenotypic switching from M1 to M2 macrophages in high-fat-diet-induced obese mice

    Noriaki Kawanishi, Hiromi Yano, Yuka Yokogawa, Katsuhiko Suzuki

    EXERCISE IMMUNOLOGY REVIEW   16   105 - 118  2010  [Refereed]

     View Summary

    Purpose: Recent studies suggest that exchange of macrophage phenotype (M1/M2) in adipose tissue is associated with chronic low-grade inflammation in obesity. M1 macrophages enhance a chronic inflammatory state in adipose tissues, whereas M2 macrophages inhibit it. Although exercise training might inhibit pro-inflammatory cytokine gene expression in adipose tissue, it remains unclear whether exercise training affects the phenotypic switch of macrophage polarization in adipose tissue. Therefore, we investigated the effect of exercise training on the macrophage phenotypic switch in adipose tissue in high-fat-induced obese mice. Methods: Male C57BL/6 mice were divided into four groups; normal diet (ND) control (n=7), ND exercise (n=7), high-fat-diet (HFD) control (n=12), and HFD exercise (n=12) groups. All exercised mice ran on a treadmill at 12-20 m/min for 60 min/day for 16 weeks. Tumor necrosis factor (TNF)-alpha, interleukin (IL)-6, F4/80, monocyte chemotactic protein (MCP)-1, CXCL14, inter-cellular adhesion molecule (ICAM)-1, vascular-cellular adhesion molecule (VCAM)- I, CD11c, CD163 and toll-like receptor (TLR)4 mRNA expressions in adipose tissue were evaluated by real time-RT-PCR. Results: In HFD mice, exercise training did not induce loss of body or adipose tissue mass, exercise training nevertheless markedly inhibited TNF-alpha and F4/80 mRNA expression in adipose tissue. The exercise training attenuated HFD-induced increase in ICAM-I mRNA expression, but not MCP-1, CXCL14 and VCAM-1 mRNA expressions. In addition, increased CD11c mRNA expression, which is a M1 macrophage specific marker, with HFD treatment was attenuated by exercise training. In contrast, although the mRNA expression of CD163, a M2 macrophage specific marker, in adipose tissue was significantly decreased by HFD, the exercise training significantly increased its expression. Also, the higher mRNA expression of TLR4, which induces pro-inflammatory cytokine production after fatty acid recognition, was strongly inhibited by the exercise training in HFD mice. Conclusion: Exercise training might induce the phenotypic switching from M1 macrophage to M2 macrophage in obese adipose tissue besides inhibiting M1 macrophage infiltration into adipose tissue. Therefore, chronic exercise might contribute to inhibit inflammation in adipose tissue via down regulation of TLR4.

  • The Different Effects of Fluid with and without Carbohydrate Ingestion on Subjective Responses of Untrained Men during Prolonged Exercise in a Hot Environment

    Toshimichi Ishijima, Hideki Hashimoto, Katsuhiko Satou, Isao Muraoka, Katsuhiko Suzuki, Mitsuru Higuchi

    JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY   55 ( 6 ) 506 - 510  2009.12  [Refereed]

     View Summary

    This study examined the effects of maintaining euhydration by ingesting fluids with or without carbohydrate on subjective responses of untrained men during prolonged exercise in a hot environment. Six healthy untrained subjects completed 90 min of cycling exercises at 55% maximal oxygen consumption (V(O2max)) in a hot environment (temperature: 28 degrees C. humidity: 50%) under three different experimental conditions. During the first trial. subjects did not ingest fluids during exercise (dehydration (DH) trial). In the second and third trials, subjects received mineral water (MW) and hypotonic fluid containing carbohydrate (HF), respectively, in amounts equaling their weight loss in the DH trial. At the end of exercise, the overall rating of perceived exertion (RPE-O) was lower in the MW and HF trials than in the DH trial (14.3+/-1.0 and 13.7+/-0.6 vs 17.7+/-1.0. p&lt;0.05. respectively). RPE-cardiovascular and RPE-legs were lower at the end of exercise in the HF trial compared with the DH trial. V(O2), heart rate (HR), and rectal temperature increased during exercise in the three trials. At the end of exercise, the drift in V(O2) was lower in the MW and HF trials than in the DH trial (304+/-41 and 339+/-40 vs 458+/-33 mL, p&lt;0.05. respectively). HR at the end of exercise in the HF trial was lower than in the DH. trial (158+/-5 vs 173+/-7 bpm. p&lt;0.05). These results suggest that maintaining euhydration during prolonged exercise in untrained men could attenuate RPE-O and that hypotonic electrolyte-carbohydrate solution could attenuate RPE-legs during exercise.

  • Exercise in, and adaptations to a cold environment have no effect on SIgA

    T. Akimoto, K. Kim, R. Yamauchi, S. Izawa, C. Hong, K. Aizawa, H. Lee, K. Suzuki

    JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS   49 ( 3 ) 315 - 319  2009.09  [Refereed]

     View Summary

    Aim. The authors hypothesized that inconsistent SIgA response to exercise is caused by the different adaptative status of subjects to a cold environment. The purposes of the study were to examine whether moderate-intense exercise in a cold environment decreases SIgA and whether adaptation to a cold environment has any effect on SIgA.
    Methods. Young male skaters, short track (N=9) and inline (N=10), participated in this study. All subjects cycled for 60 min at 65% (V) over dot(2max) in cold (ambient temperature: 5+/-1 degrees C, relative humidity 41+/-9%) and thermoneutral (ambient temperature: 21+/-1 degrees C, relative humidity 35+/-5%) conditions. Saliva samples were collected as follows: before and after 1 hour of environmental exposure; immediately, 30-min, 60-min and 120-min after the exercise.
    Results and conclusion. Salivary SIgA and saliva flow rate decreased after the exercise in both groups only in thermoneutral conditions. The SIgA secretion rate did not decrease after moderate-high intensity exercise in a cold environment, and the SIgA response to exercise was not affected by the different adaptative status of subjects to the cold environment.

  • Differences in body composition and risk of lifestyle-related diseases between young and older male rowers and sedentary controls.

    Kiyoshi Sanada, Motohiko Miyachi, Izumi Tabata, Katsuhiko Suzuki, Kenta Yamamoto, Hiroshi Kawano, Chiyoko Usui, Mitsuru Higuchi

    Journal of sports sciences   27 ( 10 ) 1027 - 34  2009.08  [International journal]

     View Summary

    The aim of this cross-sectional study was to compare body composition and risk factors of lifestyle-related diseases between young and older male rowers and sedentary controls. Healthy males aged 19-73 years participated in the study, and were divided into four groups: 26 young rowers, 24 senior rowers, 23 young sedentary controls, and 22 senior sedentary controls. Total and regional lean soft tissue, fat mass, and bone mineral density were measured using dual-energy X-ray absorptiometry. The HDL-cholesterol of senior rowers (67.4 +/- 13.4 mg . dl(-1)) was significantly (P < 0.05) higher than that of senior sedentary controls (59.2 +/- 11.9 mg . dl(-1)), while HDL-cholesterol was similar in senior rowers and young rowers (66.1 +/- 10.8 mg . dl(-1)). Arm, leg, and trunk lean soft tissue mass were significantly higher in senior rowers (5.6 +/- 0.6 kg, 18.2 +/- 1.8 kg, and 27.3 +/- 3.2 kg respectively) than in senior sedentary controls (5.1 +/- 0.4 kg, 16.3 +/- 1.4 kg, and 24.6 +/- 1.7 kg respectively; P < 0.05). Bone mineral density was also significantly higher in senior rowers than in senior sedentary controls (ribs, lumbar spine, and pelvic segments; P < 0.05). We conclude that age-related increases in the risk of lifestyle-related diseases, such as osteoporosis and sarcopenia, are attenuated in male rowers. These results suggest that regular rowing exercise may have a positive influence in the prevention of lifestyle-related diseases in older Japanese people.

    DOI PubMed

  • The effects of increased endurance training load on biomarkers of heat intolerance during intense exercise in the heat

    Chin Leong Lim, David Pyne, Peggy Horn, Amelia Kalz, Philo Saunders, Jonathan Peake, Katsuhiko Suzuki, Gary Wilson, Laurel T. Mackinnon

    APPLIED PHYSIOLOGY NUTRITION AND METABOLISM-PHYSIOLOGIE APPLIQUEE NUTRITION ET METABOLISME   34 ( 4 ) 616 - 624  2009.08  [Refereed]

     View Summary

    The effects of increased training (IT) load on plasma concentrations of lipopolysaccharides (LPS), proinflammatory cytokines, and anti-LPS antibodies during exercise in the heat were investigated in 18 male runners, who performed 14 days of normal training (NT) or 14 days of 20% IT load in 2 equal groups. Before (trial 1) and after (trial 2) the training intervention, all subjects ran at 70% maximum oxygen uptake on a treadmill under hot (35 degrees C) and humid (similar to 40%) conditions, until core temperature reached 39.5 degrees C or volitional exhaustion. Venous blood samples were drawn before, after, and 1.5 h after exercise. Plasma LPS concentration after exercise increased by 71% (trial 1, p &lt; 0.05) and 21% (trial 2) in the NT group and by 92% (trial 1, p &lt; 0.01) and 199% (trial 2, p &lt; 0.01) in the IT group. Postintervention plasma LPS concentration was 35% lower before exercise (p &lt; 0.05) and 47% lower during recovery (p &lt; 0.01) in the IT than in the NT group. Anti-LPS IgM concentration during recovery was 35% lower in the IT than in the NT group (p &lt; 0.05). Plasma interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha concentrations after exercise (IL-6, 3-7 times, p &lt; 0.01, and TNF-alpha, 33%, p &lt; 0.01) and during recovery (IL-6, 2-4 times, p &lt; 0.05, and TNF-alpha, 30%, p &lt; 0.01) were higher than at rest within each group. These data suggest that a short-term tolerable increase in training load may protect against developing endotoxemia during exercise in the heat.

    DOI

  • 保健指導としての運動指導 ―NPO法人「早稲田の杜」の設立と今後―

    真田樹義, 宮地元彦, 丸山浩平, 山元健太, 鈴木克彦, 樋口 満, 朝日 透, 坂本静男, 大坪真也, 山内 繁, 塙 智史, 片山利恵, 梅里泰正

    スポーツ科学研究   6   44-49  2009.05

  • 急性睡眠遮断後の20分または2時間仮眠の運動耐容能、内分泌機能に及ぼす影響

    小西真幸, 高木 俊, 緑川泰史, 鈴木克彦, 坂本静男

    日本臨床生理学会雑誌   39 ( 2 ) 113 - 119  2009.04

  • Effects of cold environment exposure and cold acclimatization on exercise-induced salivary cortisol response

    Shuhei Izawa, Kijin Kim, Takayuki Akimoto, Nayoung Ahn, Hoseong Lee, Katsuhiko Suzuki

    Wilderness and Environmental Medicine   20 ( 3 ) 239 - 243  2009

     View Summary

    Objective.-Considering the adverse effects of exercise-induced cortisol secretion on health in athletes, it is important to determine the environmental and individual factors that contribute to the variations in exercise-induced cortisol secretion. In this study, the effects of cold environment exposure and cold acclimatization on exercise-induced salivary cortisol responses were investigated. Methods.-Short track skaters (n = 11), who usually practice under cold conditions, and inline skaters (n = 11), who usually practice under room temperature conditions, participated in a randomized crossover study. All participants cycled for 60 minutes at 65% V̇o2 max under cold (ambient temperature: 5 ± 1°C, relative humidity 41% ± 9%) and room temperature (ambient temperature: 21 ± 1°C, relative humidity 35% ± 5%) conditions. The participants had a 120-minute bed rest recovery phase at room temperature after both exercise bouts. Cortisol levels were measured in saliva samples collected pre-exercise and postexercise at 1 minute, 30 minutes, 60 minutes, and 120 minutes. Results.-Both short track and inline skaters exhibited clear cortisol responses to exercise under cold and room temperature conditions. The magnitude of the cortisol response, however, was different between skaters and conditions. The inline skaters exhibited significantly higher cortisol values under cold conditions than under room temperature conditions (7.6 nmol/L and 4.2 nmol/L, respectively). However, the short track skaters exhibited significantly higher cortisol values under cold conditions compared to room temperature conditions (8.7 nmol/L and 5.4 nmol/L, respectively). Conclusions.-The effects of cold environment exposure on exercise-induced cortisol response were different between skaters who usually practice under cold or room temperature conditions. These results can be interpreted as acclimatization to either cold or room temperature conditions attenuating the cortisol response, suggesting that acclimatization may be beneficial in reducing the exercise-induced cortisol response. © 2009 Wilderness Medical Society.

    DOI PubMed

  • Cytokine responses to carbohydrate ingestion during recovery from exercise-induced muscle injury. (original paper)

    Ross M, Shona L, Suzuki K, Garnham A, Hawley J, Cameron-Smith D, Peake J

    J. Interferon Cytokine Res.   17 in press ( 17 )  2009

  • Salivary dehydroepiandrosterone secretion in response to acute psychosocial stress and its correlations with biological and psychological changes

    Shuhei Izawa, Nagisa Sugaya, Kentaro Shirotsuki, Kosuke Chris Yamada, Namiko Ogawa, Yuko Ouchi, Yuichiro Nagano, Katsuhiko Suzuki, Shinobu Nomura

    Biological Psychology   79 ( 3 ) 294 - 298  2008.12

     View Summary

    We investigated dehydroepiandrosterone (DHEA) secretion in response to acute psychosocial stress and the relations of DHEA secretion to cortisol secretion, cardiovascular activity, and negative mood changes. Thirty-three male students (mean age 22.6 years) were subjected to the psychosocial stress test "Trier Social Stress Test" (TSST), in which the participants were asked to deliver a speech and perform a mental arithmetic task in front of two audiences. Collections of saliva, measurements of blood pressure and heart rate, and assessments of negative mood by visual analog scales were conducted before, during, and after TSST. Acute psychosocial stress significantly increased salivary DHEA level by an average of 60% immediately after TSST. The peak of DHEA concentration preceded that of cortisol concentration by about 10 min. DHEA response was moderately correlated to cortisol response (r = .34, rs = .49) but not to cardiovascular response. Lower DHEA level and elevated cortisol/DHEA ratio during TSST were significantly and moderately correlated with increased negative mood during and after TSST. These results indicated that an acute increase in DHEA concentration under stressful situations might be partly mediated by the activity of hypothalamus-pituitary-adrenal axis and could have some significance in the improvement of negative mood. © 2008 Elsevier B.V. All rights reserved.

    DOI PubMed

  • The effects of acute exercise-induced cortisol on CCR2 expression on human monocytes

    Mitsuharu Okutsu, Katsuhiko Suzuki, Toshimichi Ishijima, Jonathan Peake, Mitsuru Higuchi

    BRAIN BEHAVIOR AND IMMUNITY   22 ( 7 ) 1066 - 1071  2008.10  [Refereed]

     View Summary

    CC-chemokine receptor 2 (CCR2) and its ligand, monocyte chemotactic protein-1 (MCP-1, also known as CCL2), are crucial for the recruitment of monocytes/macrophages to sites of inflammation. We conducted a series of experiments to investigate the relationship between stress, monocyte CCR2 expression and migration activity. First, we collected peripheral blood mononuclear cells (PBMC) from untrained subjects (n = 8) and measured CCR2 expression on CD14(+) monocytes cultured with cortisol, epinephrine and norepinephrine. Second, we collected PBMC from the subjects before and after they cycled for 60 min at 70% peak O-2 uptake (V-O2peak), and measured alterations in CCR2 expression on monocytes following exercise. Third, we cultured PBMC with serum obtained before and after exercise and the glucocorticoid antagonist RU-486 to determine the effect of cortisol on CCR2 expression in vitro. Last, we measured the ability of PBMC treated with serum or cortisol to migrate through membrane filters in response to CCL2. Cortisol (but not epinephrine or norepinephrine) increased CCR2 expression on monocytes in a dose- and time-dependent manner. Exercise did not influence CCR2 expression on PBMC, whereas incubation of PBMC with post-exercise serum significantly increased CCR2 expression. Both cortisol and post-exercise serum increased the migration of PBMC toward CCL2. The increase in CCR2 expression on PBMC following stimulation with cortisol and serum was blocked by the glucocorticoid receptor antagonist RU-486. In conclusion, cortisol released during exercise increased monocyte CCR2 expression and migration activity in vitro. These alterations may influence inflammation and regeneration of damaged tissue after acute stress. (C) 2008 Elsevier Inc. All rights reserved.

    DOI

  • Effect of carbohydrate ingestion and ambient temperature on muscle fatigue development in endurance-trained male cyclists

    Chris R. Abbiss, Jeremiah J. Peiffer, Jonathan M. Peake, Kazunori Nosaka, Katsuhiko Suzuki, David T. Martin, Paul B. Laursen

    JOURNAL OF APPLIED PHYSIOLOGY   104 ( 4 ) 1021 - 1028  2008.04  [Refereed]

     View Summary

    The aim of the present study was to determine the effect of carbohydrate (CHO; sucrose) ingestion and environmental heat on the development of fatigue and the distribution of power output during a 16.1-km cycling time trial. Ten male cyclists ((V) over dot O-2max = 61.7 +/- 5.0 ml.kg(-1).min(-1), mean +/- SD) performed four 90-min constant-pace cycling trials at 80% of second ventilatory threshold (220 +/- 12 W). Trials were conducted in temperate (18.1 +/- 0.4 degrees C) or hot (32.2 +/- 0.7 degrees C) conditions during which subjects ingested either CHO (0.96 g.kg(-1).h(-1)) or placebo (PLA) gels. All trials were followed by a 16.1-km time trial. Before and immediately after exercise, percent muscle activation was determined using superimposed electrical stimulation. Power output, integrated electromyography (iEMG) of vastus lateralis, rectal temperature, and skin temperature were recorded throughout the trial. Percent muscle activation significantly declined during the CHO and PLA trials in hot (6.0 and 6.9%, respectively) but not temperate conditions (1.9 and 2.2%, respectively). The decline in power output during the first 6 km was significantly greater during exercise in the heat. iEMG correlated significantly with power output during the CHO trials in hot and temperate conditions (r = 0.93 and 0.73; P &lt; 0.05) but not during either PLA trial. In conclusion, cyclists tended to self-select an aggressive pacing strategy (initial high intensity) in the heat.

    DOI

  • Body temperature and its effect on leukocyte mobilization, cytokines and markers of neutrophil activation during and after exercise

    Jonathan Peake, Jeremiah J. Peiffer, Chris R. Abbiss, Kazunori Nosaka, Mitsuharu Okutsu, Paul B. Laursen, Katsuhiko Suzuki

    EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY   102 ( 4 ) 391 - 401  2008.03

     View Summary

    We investigated the influence of rectal temperature on the immune system during and after exercise. Ten well-trained male cyclists completed exercise trials (90 min cycling at 60% (V) over dotO(max) + 16.1 - km time trial) on three separate occasions: once in 18 degrees C and twice in 32 degrees C. Twenty minutes after the trials in 32 degrees C, the cyclists sat for - 20 min in cold water (14 degrees C) on one occasion, whereas on another occasion they sat at room temperature. Rectal temperature increased significantly during cycling in both conditions, and was significantly higher after cycling in 32 degrees C than in 18 degrees C (P &lt; 0.05). Leukocyte counts increased significantly during cycling but did not differ between the conditions. The concentrations of serum interleukin (IL)-6, IL-8 and IL-10, plasma catecholamines, granulocyte-colony stimulating factor, myeloperoxidase and calprotectin increased significantly following cycling in both conditions. The concentrations of serum IL-8 (25%), IL-10 (120%), IL-1 receptor antagonist (70%), tumour necrosis factor-alpha (17%), plasma myeloperoxidase (26%) and norepinephrine (130%) were significantly higher after cycling in 32 degrees C than in 18 degrees C. During recovery from exercise in 32 degrees C, rectal temperature was significantly lower in response to sitting in cold water than at room temperature. However, immune changes during 90 min of recovery did not differ significantly between sitting in cold water and at room temperature. The greater rise in rectal temperature during exercise in 32 degrees C increased the concentrations of serum IL-8, IL-10, IL-1ra, TNF-alpha and plasma myeloperoxidase, whereas the greater decline in rectal temperature during cold water immersion after exercise did not affect immune responses.

    DOI

  • Galvao DA, Nosaka K, Taaffe DR, Peake J, Spry N, Suzuki K, Yamaya K, Newton RU.

    Endocrine, immune responses to, resistance training in, rostate cancer patients. (original pap

    Prostate Cancer Prostatic Dis.   11   160 - 165  2008

  • Comparison of metabolic substances between exercise and cold exposure in skaters (original paper).

    Hong JH, Kim HJ, Kim KJ, Suzuki K, Lee IS

    J Physiol Anthropol.   27   273 - 281  2008

  • Effect of cold acclimation on antioxidant status in cold acclimated skaters (original paper).

    Hong JH, Kim KJ, Suzuki K, Lee IS

    J Physiol Anthropol.   27   255 - 262  2008

  • Carbohydrate gel ingestion and immunoendocrine responses to cycling in temperate and hot conditions. (original paper)

    Peake J, Peiffer JJ, Abbiss CR, Nosaka K, Laursen PB, Suzuki K

    Int. J. Sport Nutr. Exerc. Metab.   18   229 - 246  2008

  • The association of elevated reactive oxygen species levels from neutrophils with low-grade inflammation in the elderly. (original paper)

    Ogawa K, Suzuki K, Okutsu M, Yamazaki K, Shinkai S

    Immun Ageing   5 (in press) ( 13 )  2008

  • Bovine colostrum modulates cytokine production in human peripheral blood mononuclear cells stimulated with LPS and PMA. (original paper)

    Shing CM, Peake JM, Suzuki K, Jenkins DG

    J. Interferon Cytokine Res.   29 ( 1 ) 37 - 44  2008

  • Salivary dehydroepiandrosterone secretion in response to acute psychological stress and its correlations with biological and psychological changes. (original paper)

    Izawa S, Sugaya N, Shirotsuki K, Yamada KC, Ogawa S, Sugaya N, Shirotsuki K, Yamada KC, Ogawa N, Ouchi Y, Nagano Y, Suzuki K, Nomura S

    Biol Psychol   89 ( 3 ) 294 - 298  2008

  • 220. 長時間運動中の低浸透圧飲料摂取が運動前後の最大随意収縮に及ぼす影響(栄養・消化,第62回日本体力医学会大会)

    石島 寿道, 彼末 一之, 村岡 功, 鈴木 克彦, 薄井 澄誉子, 橋本 秀紀, 佐藤 克彦, 岡本 武久, 樋口 満

    体力科學   56 ( 6 ) 688 - 688  2007.12

    CiNii

  • 唾液中コルチゾールの測定キットの比較―唾液中・血漿中コルチゾールの相関ならびに測定法間の比較―

    井澤修平, 鈴木克彦

    日本補完代替医療学会誌   4 ( 3 ) 113 - 118  2007.10  [Refereed]

    DOI CiNii

  • 唾液を用いたストレス評価―採取及び測定手続きと各唾液中物質の特徴―

    井澤修平, 城月健太郎, 菅谷 渚, 小川奈美子, 鈴木克彦, 野村 忍

    日本補完代替医療学会誌   4 ( 3 ) 91 - 101  2007.10  [Refereed]

    DOI CiNii

  • The influence of antioxidant supplementation on markers of inflammation and the relationship to oxidative stress after exercise

    Jonathan M. Peake, Katsuhiko Suzuki, Jeff S. Coombes

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY   18 ( 6 ) 357 - 371  2007.06  [Refereed]

     View Summary

    Interest in the relationship between inflammation and oxidative stress has increased dramatically in recent years, not only within the clinical setting but also in the fields of exercise biochemistry and immunology. Inflammation and oxidative stress share a common role in the etiology of a variety Of Chronic diseases. During exercise, inflammation and oxidative stress are linked via muscle metabolism and muscle damage. Because oxidative stress and inflammation have traditionally been associated with fatigue and impaired recovery from exercise, research has focused on nutritional strategies aimed at reducing these effects. In this review, we have evaluated the findings of studies involving antioxidant supplementation on alterations in markers of inflammation (e.g., cytokines, C-reactive protein and cortisol). This review focuses predominantly on the role of reactive oxygen and nitrogen species generated from muscle metabolism and muscle damage during exercise and on the modulatory effects of antioxidant supplements. Furthermore, we have analyzed the influence of factors such as the dose, timing, supplementation period and bioavailability of antioxidant nutrients. (C) 2007 Elsevier Inc. All rights reserved.

    DOI

  • 20歳から80歳までの男女1463名を対象とした骨格筋量と換気閾値との関係.

    樋口満, 真田樹義, 鈴木克彦

    厚生労働科学研究費補助金 平成18年度総括・分担研究報告書     22-25  2007.03

  • 肥満関連遺伝子、身体組成、有酸素能力がメタボリックシンドロームに及ぼす影響.

    樋口満, 真田樹義, 鈴木克彦

    厚生労働科学研究費補助金 平成18年度総括・分担研究報告書     17-21  2007.03

  • Reduction in resting plasma granulysin as a marker of increased training load

    Cecilia M. Shing, Kazuyuki Ogawa, Xiurnin Zhang, Ryoichi Nagatomi, Jonathan M. Peake, Katsuhiko Suzuki, David G. Jenkins, Jeff S. Coornbes

    EXERCISE IMMUNOLOGY REVIEW   13   89 - 99  2007  [Refereed]

     View Summary

    Granulysin is a cytolytic granule protein released by natural killer cells and activated cytotoxic T lymphocytes. The influence of exercise training on circulating granulysin concentration is unknown, as is the relationship between granulysin concentration, natural killer cell number and natural killer cell cytotoxicity. We examined changes in plasma granulysin concentration, natural killer cell number and cytotoxicity following acute exercise and different training loads. Fifteen highly trained male cyclists completed a baseline 40-km cycle time trial (TT(40)1) followed by five weeks of normal training and a repeat time trial (TT(40)2). The cyclists then completed four days of high intensity training followed by another time trial (TT(40)3) on day five. Following one final week of normal training cyclists completed another time trial (TT(40)4). Fasting venous blood was collected before and after each time trial to determine granulysin concentration, natural killer cell number and natural killer cell cytotoxicity. Granulysin concentration increased significantly after each time trial (P&lt;0.001). Pre-exercise granulysin concentration for TT(40)3 was significantly lower than pre-exercise concentration for TT(40)1 (-20.3 +/- 7.5%, P&lt;0.026), TT(40)2 (-16.7 +/- 4.3%, P&lt;0.003) and TT(40)4 (-21 +/- 4.2%, P&lt;0.001). Circulating natural killer cell numbers also increased significantly post-exercise for each time trial (P&lt;0.001), however there was no significant difference across TT40 (P&gt;0.05). Exercise did not significantly alter natural killer cell cytotoxicity on a per cell basis, and there were no significant differences between the four time trials. In conclusion, plasma granulysin concentration increases following moderate duration, strenuous exercise and is decreased in response to a short-term period of intensified training.

  • Exercise-induced NK cells display exercise-associated eHSP-70. (original paper)

    Horn P, Kalz A, Lim CL, Pyne D, Saunders P, Mackinnon L, Peake J, Suzuki K

    Exerc. Immunol. Rev.   13   100 - 111  2007

  • 265. 暑熱環境下における90分間運動中の自由なハイポトニック飲料摂取が血漿量の変化に及ぼす影響(栄養・消化, 第61回 日本体力医学会大会)

    呉 泰雄, 樋口 満, 彼末 一之, 薄井 澄誉子, 石島 寿道, 村岡 功, 坂本 静男, 光田 博充, 石井 千広, 鈴木 克彦

    体力科學   55 ( 6 ) 711 - 711  2006.12

    CiNii

  • Changes in markers of muscle damage, inflammation and HSP70 after an Ironman triathlon race

    Katsuhiko Suzuki, Jonathan Peake, Kazunori Nosaka, Mitsuharu Okutsu, Chris R. Abbiss, Rob Surriano, David Bishop, Marc J. Quod, Hamilton Lee, David T. Martin, Paul B. Laursen

    EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY   98 ( 6 ) 525 - 534  2006.12  [Refereed]

     View Summary

    We investigated the effects of an Ironman triathlon race on markers of muscle damage, inflammation and heat shock protein 70 (HSP70). Nine well-trained male triathletes (mean +/- SD age 34 +/- 5 years; VO2peak 66.4 ml kg(-1) min(-1)) participated in the 2004 Western Australia Ironman triathlon race (3.8 km swim, 180 km cycle, 42.2 km run). We assessed jump height, muscle strength and soreness, and collected venous blood samples 2 days before the race, within 30 min and 14-20 h after the race. Plasma samples were analysed for muscle proteins, acute phase proteins, cytokines, heat shock protein 70 (HSP70), and clinical biochemical variables related to dehydration, haemolysis, liver and renal functions. Muscular strength and jump height decreased significantly (P &lt; 0.05) after the race, whereas muscle soreness and the plasma concentrations of muscle proteins increased. The cytokines interleukin (IL)-1 receptor antagonist, IL-6 and IL-10, and HSP70 increased markedly after the race, while IL-12p40 and granulocyte colony-stimulating factor (G-CSF) were also elevated. IL-4, IL-1 beta and tumour necrosis factor-alpha did not change significantly, despite elevated C-reactive protein and serum amyloid protein A on the day after the race. Plasma creatinine, uric acid and total bilirubin concentrations and gamma-glutamyl transferase activity also changed after the race. In conclusion, despite evidence of muscle damage and an acute phase response after the race, the pro-inflammatory cytokine response was minimal and anti-inflammatory cytokines were induced. HSP70 is released into the circulation as a function of exercise duration.

    DOI

  • Systemic inflammatory responses to maximal versus submaximal lengthening contractions of the elbow flexors. (original paper)

    Peake J, Nosaka K, Muthalib M, Suzuki K

    Exerc. Immunol. Rev.   12   72 - 85  2006

  • Resistance training reduces treatment side-effects in prostate cancer patients. (original paper)

    Galvao DA, Nosaka K, Taaffe DR, Spry N, Kristjanson LJ, McGuigan MR, Suzuki K, Yamaya K, Newton RU

    Med. Sci. Sports Exerc.   38   2045 - 2052  2006

  • Effects of bovine colostrum supplementation on immune variables in highly-trained cyclists. (original paper)

    Shing CM, Peake J, Suzuki K, Okutsu M, Pereira R, Stevenson L, Jenkins DG, Coombes JS

    J. Appl. Physiol.   102   1113 - 1122  2006

  • Plasma cytokine changes in relation to exercise intensity and muscle damage

    JM Peake, K Suzuki, M Hordern, G Wilson, K Nosaka, JS Coombes

    EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY   95 ( 5-6 ) 514 - 521  2005.12  [Refereed]

     View Summary

    The purpose of this study was to compare the effects of exercise intensity and exercise-induced muscle damage on changes in anti-inflammatory cytokines and other inflammatory mediators. Nine well-trained male runners completed three different exercise trials on separate occasions: ( 1) level treadmill running at 60% VO2max (moderate-intensity trial) for 60 min; (2) level treadmill running at 85% VO2max (high-intensity trial) for 60 min; (3) downhill treadmill running ( - 10% gradient) at 60% VO2 max (downhill running trial) for 45 min. Blood was sampled before, immediately after and 1 h after exercise. Plasma was analyzed for interleukin-1 receptor antagonist (IL-1ra), IL-4, IL-5, IL-10, IL-12p40, IL-13, monocyte chemotactic protein-1 (MCP-1), prostaglandin E-2, leukotriene B-4 and heat shock protein 70 (HSP70). The plasma concentrations of IL-1ra, IL-12p40, MCP-1 and HSP70 increased significantly (P&lt; 0.05) after all three trials. Plasma prostaglandin E-2 concentration increased significantly after the downhill running and high-intensity trials, while plasma IL-10 concentration increased significantly only after the high-intensity trial. IL-4 and leukotriene B4 did not increase significantly after exercise. Plasma IL-1ra and IL-10 concentrations were significantly higher ( P&lt; 0.05) after the high-intensity trial than after both the moderate-intensity and downhill running trials. Therefore, following exercise up to 1 h duration, exercise intensity appears to have a greater effect on anti-inflammatory cytokine production than exercise-induced muscle damage.

    DOI

  • Exercise-induced muscle damage, plasma cytokines, and markers of neutrophil activation

    JM Peake, K Suzuki, G Wilson, M Hordern, K Nosaka, L Mackinnon, JS Coombes

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   37 ( 5 ) 737 - 745  2005.05  [Refereed]

     View Summary

    Introduction: Unaccustomed eccentric exercise often results in muscle damage and neutrophil activation. We examined changes in plasma cytokines stress hormones, creatine kinase activity and myoglobin concentration, neutrophil surface receptor expression, degranulation. and the capacity of neutrophils to generate reactive oxygen species in response to in vitro stimulation after downhill running. Methods: Ten well-trained male runners ran downhill on a treadmill at a gradient of - 10% for 45 min at 60% VO2max.. Blood was sampled immediately before (PRE) and after (POST), 1 h (1 h POST), and 24 h (24 h POST) after exercise. Results: At POST, there were significant increases (P &LT; 0.01) in neutrophil count (32%), plasma interleukin (IL)-6 concentration (460%), myoglobin (Mb) concentration (1100%), and creatine kinase (CK) activity (40%). At 1 h POST, there were further increases above preexercise values for neutrophil count (85%), plasma Mb levels (1800%), and CK activity (56%), and plasma IL-6 concentration remained above preexercise values (410%) (P &LT; 0.01). At 24 h POST, neutrophil counts and plasma IL-6 levels had returned to baseline, whereas plasma Mb concentration (100%) and CK activity (420%) were elevated above preexercise values (P &LT; 0.01). There were no significant changes in neutrophil receptor expression, degranulation and respiratory burst activity, and plasma IL-8 and granulocyte-colony stimulating factor concentrations at any time after exercise. Neutrophil count correlated with plasma Mb concentration at POST (r = 0.64, P &LT; 0.05), and with plasma CK activity at POST (r = 0.83, P &LT; 0.01) and I h POST (r = 0.78, P &LT; 0.01). Conclusion: Neutrophil activation remains unchanged after downhill running in well-trained runners, despite increases in plasma markers of muscle damage.

    DOI

  • Characterization of inflammatory responses to eccentric exercise in humans

    J Peake, K Nosaka, K Suzuki

    EXERCISE IMMUNOLOGY REVIEW   11   64 - 85  2005  [Refereed]

     View Summary

    Eccentric exercise commonly results in muscle damage. The primary sequence of events leading to exercise-induced muscle damage is believed to involve initial mechanical disruption of sarcomeres, followed by impaired excitation-contraction coupling and calcium signaling, and finally, activation of calcium-sensitive degradation pathways. Muscle damage is characterized by ultrastructural changes to muscle architecture, increased muscle proteins and enzymes in the bloodstream, loss of muscular strength and range of motion and muscle soreness. The inflammatory response to exercise-induced muscle damage is characterized by leukocyte infiltration and production of pro-inflammatory cytokines within damaged muscle tissue, systemic release of leukocytes and cytokines, in addition to alterations in leukocyte receptor expression and functional activity. Current evidence suggests that inflammatory responses to muscle damage are dependent on the type of eccentric exercise, previous eccentric loading (repeated bouts), age and gender. Circulating neutrophil counts and systemic cytokine responses are greater after eccentric exercise using a large muscle mass (e.g. downhill running, eccentric cycling) than after other types of eccentric exercise involving a smaller muscle mass. After an initial bout of eccentric exercise, circulating leukocyte counts and cell surface receptor expression are attenuated. Leukocyte and cytokine responses to eccentric exercise are impaired in elderly individuals, while cellular infiltration into skeletal muscle is greater in human females than males after eccentric exercise. Whether alterations in intracellular calcium homeostasis influence inflammatory responses to muscle damage is uncertain. Furthermore, the effects of antioxidant supplements are variable, and the limited data available indicates that anti-inflammatory drugs largely have no influence on inflammatory responses to eccentric exercise. In this review, we compare local versus systemic inflammatory responses, and discuss some of the possible mechanisms regulating the inflammatory responses to exercise-induced muscle damage in humans.

  • Changes in neutrophil surface receptor expression, degranulation, and respiratory burst activity after moderate- and high-intensity exercise

    J Peake, G Wilson, M Hordern, K Suzuki, K Yamaya, K Nosaka, L Mackinnon, JS Coombes

    JOURNAL OF APPLIED PHYSIOLOGY   97 ( 2 ) 612 - 618  2004.08  [Refereed]

     View Summary

    Intense exercise stimulates the systemic release of a variety of factors that alter neutrophil surface receptor expression and functional activity. These alterations may influence resistance to infection after intense exercise. The aim of this study was to examine the influence of exercise intensity on neutrophil receptor expression, degranulation (measured by plasma and intracellular myeloperoxidase concentrations), and respiratory burst activity. Ten well-trained male runners ran on a treadmill for 60 min at 60% [moderate-intensity exercise (MI)] and 85% maximal oxygen consumption [high-intensity exercise (HI)]. Blood was drawn immediately before and after exercise and at 1 h postexercise. Immediately after HI, the expression of the neutrophil receptor CD 16 was significantly below preexercise values (P &lt; 0.01), whereas MI significantly reduced CD35 expression below preexercise values (P &lt; 0.05). One hour after exercise at both intensities, there was a significant decline in CD llb expression (P &lt; 0.05) and a further decrease in CD16 expression compared with preexercise values (P &lt; 0.01). CD16 expression was lower 1 h after HI than I h after MI (P &lt; 0.01). Immediately after HI, intracellular myeloperoxidase concentration was less than preexercise values (P &lt; 0.01), whereas plasma myeloperoxidase concentration was greater (P &lt; 0.01), indicating that HI stimulated neutrophil degranulation. Plasma myeloperoxidase concentration was higher immediately after HI than after MI (P &lt; 0.01). Neutrophil respiratory burst activity increased after HI (P &lt; 0.01). In summary, both MI and HI reduced neutrophil surface receptor expression. Although CD16 expression was reduced to a greater extent after III, this reduction did not impair neutrophil degranulation and respiratory burst activity.

    DOI

  • 運動と免疫(総説)

    鈴木克彦

    日本補完代替医療学会誌   1   31 - 40  2004

  • Neutrophil activation, antioxidant supplements and exercise-induced oxidative stress

    J Peake, K Suzuki

    EXERCISE IMMUNOLOGY REVIEW   10   129 - 141  2004  [Refereed]

     View Summary

    Neutrophils produce free radicals known as reactive oxygen species (ROS), which assist in the clearance of damaged host tissue. Tissue damage may occur during exercise due to muscle damage, thermal stress and ischaemia/reperfusion. When produced in excess, neutrophil-derived ROS may overwhelm the body's endogenous antioxidant defence mechanisms, and this can lead to oxidative stress. There is increasing evidence for links between oxidative.stress and a variety of pathological disorders such as cardiovascular diseases, cancer, chronic inflammatory diseases and post-ischaemic organ injury. A small number of studies have investigated whether there is a link between neutrophil activation and oxidative stress during exercise. In this review, we have summarised the findings of these studies. Exercise promotes the release of neutrophils into the circulation, and some evidence suggests that neutrophils mobilised after exercise have an enhanced capacity to generate some forms of ROS when stimulated in vitro. Neutrophil activation during exercise may challenge endogenous antioxidant defence mechanisms, but does not appear to increase lipid markers of oxidative stress to any significant degree, at least in the circulation. Antioxidant supplements such as N-acetylcysteine are effective at attenuating increases in the capacity of neutrophils to generate ROS when stimulated in vitro, whereas vitamin E reduces tissue infiltration of neutrophils during exercise. Free radicals generated during intense exercise may lead to DNA damage in leukocytes, but it is unknown if this damage is the result of neutrophil activation. Exercise enhances the expression of inducible haem (heme)-oxygenase (HO-1) in neutrophils after exercise, however, it is uncertain whether oxidative stress is the stimulus for this response.

  • Impact of a competitive marathon race on systemic cytokine and neutrophil responses

    K Suzuki, S Nakaji, M Yamada, Q Liu, S Kurakake, N Okamura, T Kumae, T Umeda, K Sugawara

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   35 ( 2 ) 348 - 355  2003.02  [Refereed]

     View Summary

    Purpose: To investigate whether cytokines and neutrophils mediate exercise-related pathogenesis, we examined their responses and possible association after exhaustive exercise. Methods: Plasma and urine samples were obtained from 10 male runners before and after a 42.195-km marathon race. Major cytokines and neutrophil activation markers [myeloperoxidase (MPO) and lactoferrin (LTF)] were measured by enzyme-linked immunosorbent assays. Functional modulation of standard neutrophils and monocytes by plasma was determined in vitro on their luminol-dependent chemiluminescence responses. Results: The race induced peripheral neutrophilia accompanied by an increase in band neutrophils and monocytosis. Plasma MPO and LTF concentrations increased significantly by 1.8 and 1.4 times after the race. There was a greater increase in urine concentrations of MPO and LTF, 12.3 and 3.5 times after exercise, respectively, suggesting that neutrophil activation occurred and that renal clearance exceeded the increase in plasma concentrations. Plasma interleukin (IL)-6, IL-8, IL-10, granulocyte colony-stimulating factor (G-CSF), macrophage CSF (M-CSF), and monocyte chemotactic protein 1 (MCP-1) increased significantly after the race, and urine IL-beta, IL-6, G-CSF, M-CSF, and MCP-1 increased significantly. The plasma IL-6 responses correlated with the increases of band neutrophil count (r = 0.860, P &lt; 0.01), suggesting IL-6-mediated bone marrow release of neutrophils. Furthermore, the increases in urine MPO concentration were correlated with increases in urine IL-6 (r = 0.868, P &lt; 0.01) and G-CSF (r = 0.875, P &lt; 0.01), suggesting that these cytokines promoted neutrophil activation in vivo. However, in vitro preincubation of neutrophils and monocytes with postexercise plasma could not cause priming responses, possibly because of the exercise-induced enhancement of plasma antioxidant activity. Conclusion: Although many cytokines recruiting and priming neutrophils and monocytes were secreted and functional after exhaustive exercise, overwhelming antioxidant and anti inflammatory defenses were induced, preventing exercise-induced oxidative stress.

    DOI

  • Exhaustive exercise and type-1/type-2 cytokine balance with special focus on interleukin-12 p40/p70

    K Suzuki, S Nakaji, S Kurakake, M Totsuka, K Sato, T Kuriyama, H Fujimoto, K Shibusawa, K Machida, K Sugawara

    EXERCISE IMMUNOLOGY REVIEW   9   48 - 57  2003  [Refereed]

     View Summary

    It is known that interleukin (IL)-12 p70 promotes the differentiation of type-1 helper T (Th1) cells, which produce type-1 cytokines such as IL-2 and interferon (IFN), thereby supporting cellular immunity, whereas IL-12 p40 acts as an antagonist of IL-12 p70. In contrast, IL-4 and IL-6 promote the differentiation of Th2 cells, which produce type-2 cytokines IL-4, IL-6 and IL-10, induce humoral immunity and are involved in allergic reactions. Exhaustive exercise causes the suppression of T lymphocyte activity while asthmatic and allergic diseases are subclinically more prevalent in athletes. One of the mechanisms behind these observations might be a lower type-1 and higher type-2 cytokine balance, which we previously demonstrated to occur after exhaustive exercise. In the present study, we investigated the type-1/type-2 cytokine balance by measuring plasma concentrations of IL-2, IL-4, IL-5, IL-10, tumor necrosis factor (TNF)-alpha and IFN-gamma with microparticle-based flow cytometric technology. IL-5, IL-6 and IL-13 were measured by enzyme-linked immunosorbent assay (ELISA). IL-12 p40 and p70 were measured separately, also by ELISA. Plasma IL-12 p40 concentration rose significantly after maximal exercise and to an even greater extent after a marathon race. Conversely, plasma IL-12 p70 could not be detected even using two different assays. The marathon race caused a marked increase in the plasma concentrations of IL-6 and IL-10. Their responses were correlated (r=0.78, p&lt;0.01), indicating that IL-6 is an inducer of IL-10, and may partly induce the type-1 &lt; type-2 cytokine balance. With the exception of one study involving maximal exercise, other studies have failed to show any change in circulating IL-12 concentration with exercise. The present study demonstrated that IL-12 p40 was present in excess of p70 especially after exercise. This may be one of the mechanisms behind several phenomena including cellular immunosuppression, an increase in the relative proportion of type-2 cytokines following exhaustive exercise, and the higher incidence of infections and allergic disorders in regularly exercising endurance athletes.

  • Break point of serum creatine kinase release after endurance exercise

    M Totsuka, S Nakaji, K Suzuki, K Sugawara, K Sato

    JOURNAL OF APPLIED PHYSIOLOGY   93 ( 4 ) 1280 - 1286  2002.10  [Refereed]

     View Summary

    We investigated whether there is a break point of creatine kinase (CK) release after daily endurance exercise and whether CK response depends on individual physical characteristics. Fifteen healthy young men performed 90 min of bicycle exercise for 3 consecutive days. Body composition, properties of the quadriceps femoris muscle (QFM), and aerobic and anaerobic capacities were estimated before the test. Blood samples were obtained 22 times during the experimental period. Endurance exercise significantly elevated serum CK from 3 h after the first exercise session (P &lt; 0.05) and gradually increased thereafter. Subjects were classified into two groups according to their peak CK values: high responders (HR; &gt;500 IU/l of CK) and low responders (LR; &lt;300 IU/l of CK). Peak CK values during the experimental period correlated (P &lt; 0.01) with workload/cross-sectional area of the QFM (r = 0.658), workload/volume of the QFM (r = 0.648), and knee extensor strength/body mass (r = -0.634); however, the HR and LR groups were separated in each variable. Thus the break point of CK release after endurance exercise under these conditions is 300-500 IU/l, two or three times higher than in the resting condition, and is associated with properties of the QFM.

    DOI

  • Raised plasma G-CSF and IL-6 after exercise may play a role in neutrophil mobilization into the circulation

    M Yamada, K Suzuki, S Kudo, M Totsuka, S Nakaji, K Sugawara

    JOURNAL OF APPLIED PHYSIOLOGY   92 ( 5 ) 1789 - 1794  2002.05  [Refereed]

     View Summary

    We examined the hypothesis that the short, intensive exercise-induced increase in circulating neutrophil counts is affected by the interaction between the endocrine and immune systems. Twelve male winter-sports athletes underwent a maximal exercise test on a treadmill. Blood samples were collected before, immediately after (Post), and 1 h (Post 1 h) and 2 h (Post 2 h) after the exercise. The neutrophil counts increased significantly at Post 1 h (P &lt; 0.05) and remained significantly high even at Post 2 h (P &lt; 0.05), showing a leftward shift. Plasma granulocyte colony-stimulating factor (G-CSF) increased at Post (P &lt; 0.05), and interleukin-6 (IL-6) increased at Post 1 h (P &lt; 0.05). Plasma G-CSF at Post significantly correlated with the numbers of both neutrophils and stab cells at Post 1 h (P &lt; 0.05). Plasma IL-6 at Post 1 h levels also correlated significantly with the number of neutrophils at Post 2 h (P &lt; 0.05). The increase in the levels of plasma G-CSF and IL-6 after intensive exercise may play a role in the mobilization of neutrophils into the circulatory system.

    DOI

  • Depressed humoral immunity after weight reduction in competitive judoists

    S Ohta, S Nakaji, K Suzuki, M Totsuka, T Umeda, K Sugawara

    LUMINESCENCE   17 ( 3 ) 150 - 157  2002.05  [Refereed]

     View Summary

    We studied changes in serum opsonic activity (SOA) in male judoists who were engaged in active weight reduction. Serum immunoglobulins, complements and SOA, measured by neutrophil-associated chemiluminescence responses. were investigated 20 days, 7 days and I day before a competition and 5 days after the competition, In addition, muscle strength and anaerobic work capacity, as well as body composition, were also determined. A dietary survey was conducted daily during the observation period, Body weight decreased by 4.2 kg over 19 days. SOA significantly decreased 5 days alter the competition. as well as the concentrations of serum immunoglobulins. complements and total proteins. These trends were noted in the marked weight reduction group (i.e. reduction weight of body fat/body fat weight before weight reduction greater than or equal to25%() more than the slight reduction group (&lt;25%). Depressed SOA was closely correlated with the decreased concentrations of intmunoglobulins and complements. These results suggest that the decrease in immunoglobulins and complements following weight reduction is associated with reduced SOA, which might cause susceptibility to infections. This study demonstrated that such immunosuppression appeared in the recovery period after the competition rather than immediately before the competition. Copyright (C) 2002 John Wiley Sons. Ltd.

    DOI

  • 運動療法の新しい取り組み—そのメカニズムと有用性について—

    鈴木克彦ほか

    治療   84   3037 - 3041  2002

  • メカニズムをさぐる Ⅳサイトカイン:特集/運動は免疫能を高めるか?

    鈴木克彦ほか

    臨床スポーツ医学   19   1311 - 1318  2002

  • Relationships between lifestyle factors and neutrophil functions in the elderly.

    Tsukamoto K, Suzuki K

    J. Clin. Lab. Anal.   16   266 - 272  2002

  • Systemic inflammatory response to exhaustive exercise. Cytokine kinetics

    K Suzuki, S Nakaji, M Yamada, M Totsuka, K Sato, K Sugawara

    EXERCISE IMMUNOLOGY REVIEW   8   6 - 48  2002  [Refereed]

     View Summary

    It has been documented that strenuous exercise not only induces pyrogenesis but also elicits mobilization and functional augmentation of neutrophils and monocytes whereas it suppresses cellular immunity leading to increased susceptibility to infections. As mediators Of these phenomena, cytokines released into the circulation have been a recent focus of attention. Indeed, there are as many as one hundred original reports concerning exercise and cytokines, and half of them have been published in rapid succession from 2000, resulting in a tremendous accumulation of new knowledge within such a short term.
    The first aim of this review is to comprehensively summarize previous studies on systemic cytokine kinetics following exercise, with a special focus on reproducibility and quantitative comparison in human studies using specific immunoassays. Although tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta have traditionally been understood to be the main inducer cytokines of acute phase reactions, the majority of studies have shown that the circulating concentration of these cytokines is either unchanged following exercise, or exhibits relatively small, delayed increments. Plasma interferon (IFN)-alpha and IFN-gamma do not appear to change following exercise, whereas IL-2 decreases after endurance exercise. The small changes of these proinflammatory and immunomodulatory cytokines could well be mediated by anti-inflammatory cytokines such as IL-1 receptor antagonist (IL-1ra), IL-6 and IL-10 and cytokine inhibitors (cortisol, prostaglandin E-2 and soluble receptors against TNF and IL-2), which are known to increase markedly in the circulation following endurance exercise. Moreover it has been recently demonstrated that endurance exercise induces systemic release of granulocyte colony-stimulating factor (G-CSF), macrophage CSF (M-CSF), IL-8 and monocyte chemotactic protein 1 (MCP-1).
    Although the majority of available data have been obtained following prolonged exercise, it remains to be elucidated whether short-duration intensive exercise also causes rapid systemic cytokine release. In addition, there have been few studies that have simultaneously compared the extent of each cytokine response to exercise from a wider perspective. The second aim of this study was to examine possible changes of not only plasma but also urine concentrations of a broad spectrum of cytokines (16 kinds) following maximal exercise, including the time course of recovery. Although plasma TNF-alpha could not be detected throughout, it was present in urine 2 It after exercise. Plasma IL-1 beta rose significantly 2 h after exercise, but plasma IL-1ra increased more rapidly and markedly than IL-1 beta, thus IL-1 bioactivity should be blocked at least in the circulation. Although there was only a trend toward increased plasma IL-6 concentrations after exercise, urine IL-6 rose significantly I h after exercise, indicating that IL-6 was released systemically but eliminated rapidly into the urine. Furthermore, it is shown for the first time that plasma and urine IL-4 concentrations were significantly elevated 2 h after exercise. Therefore, it is possible that anti-inflammatory cytokines might be released into the circulation as a regulatory mode of the cytokine network for adaptation against systemic inflammatory stress. Additionally, we have demonstrated that plasma concentrations of G-CSF granulocyte-macrophage CSF (GM-CSF), M-CSF IL-8 and MCP-1 increased immediately after short-duration exercise and that the urine concentrations of these cytokines were much more pronounced than the changes observed in plasma.
    In conclusion, cytokines that are considered to induce systemic bioactivity following exercise are not only anti-inflammatory cytokines but also colony-stimulating factors and chemokines, which were secreted in an earlier phase of exercise without the kinetic involvement of traditional proinflammatory cytokines. Although the wider physiological and pathological implications are still not clearly understood, these cytokine kinetics may partly explain suppressed cell-mediated immunity and increased allergic reactions derived from a lower type-1 to type-2 cytokine ratio, along with mobilization and functional augmentation of neutrophils and monocytes. The sources and stimuli of cytokine production are not fully elucidated at present, but several hypotheses based on recent experimental evidence are discussed in this review herein.

  • Effects of zinc on the reactive oxygen species generating capacity of human neutrophils and on the serum opsonic activity in vitro

    H Hasegawa, K Suzuki, K Suzuki, S Nakaji, K Sugawara

    LUMINESCENCE   15 ( 5 ) 321 - 327  2000.09  [Refereed]

     View Summary

    To investigate the effects of zinc on non-specific immune functions, we used the chemiluminescence method to examine the capacity of human neutrophils to produce reactive oxygen species and accompanying serum opsonic activity. When neutrophils were stimulated with both opsonized zymosan and phorbol myristate acetate in the presence of 1-10(-3) mmol/L zinc lucigenin-dependent CL responses were stable or declined, whereas luminol-dependent CL responses were significantly enhanced. The results suggest that zinc activates protein kinase C and promotes MPO degranulation and ROS metabolism, especially in hypochlorous acid production, which have the direct action of causing microbial death. Further, the lucigen-dependent CL response stimulated with OZ was strongly enhanced by anti-MPO antibodies, whereas the enhancement was less in the presence of zinc, suggesting that zinc may suppress the receptor-mediated signal transduction process. Both responses were inhibited at 10 mmol/L. Serum opsonic activity was enhanced by zinc at 10(-4) and 10(-3) mmol/L but reduced at 10 mmol/L. These data indicate that addition of zinc around and above normal physiological concentrations facilitates neutrophil functional activity and serum opsonic activity, whereas these are inhibited by a lack of zinc or an excessive amount, suggesting that zinc is essential for optimal functioning of nonspecific immunity. Copyright (C) 2000 John Wiley & Sons, Ltd.

  • Effect of decaglycerol monooleate on phagocytosis and respiratory burst activity of human neutrophils: an in vitro study

    Q Liu, K Suzuki, S Kudo, M Yamada, K Kowatari, T Umeda, S Nakaji, K Sugawara

    FOOD AND CHEMICAL TOXICOLOGY   38 ( 5 ) 423 - 428  2000.05  [Refereed]

     View Summary

    Decaglycerol monooleate (DGMO), a type of polyglycerol esters of fatty acids (PGEF), was evaluated for its in vitro effect on phagocytosis and respiratory burst activity of isolated human neutrophils using flow cytometric assay. Opsonized zymosan particles labelled with FITC (FITC-OZ) were employed as an indicator of phagocytosis. Fluorescence of FITC-OZ attached on to the surface of neutrophils was quenched by addition of trypan blue solution. After 10 minutes of incubation with DGMO up to a concentration of 10 mg/ml, neutrophil phagocytosis was not affected markedly. At the same time, the DGMO emulsion left little influence on complement receptor type three (CR3) that is associated with phagocytosis. On the other hand, oxidation of hydroethidine, which was used as an indicator of intracellular generation of reactive oxygen species (mainly for superoxide anion), was significantly inhibited by DGMO over 1 mg/ml. However, this phenomenon was not seen in DGMO-treated neutrophils when DGMO was removed after incubation. The present data suggest that DGMO does not affect phagocytosis of human neutrophils but down-regulates respiratory burst activity. (C) 2000 Published by Elsevier Science Ltd. All rights reserved.

  • Circulating cytokines and hormones with immunosuppressive but neutrophil-priming potentials rise after endurance exercise in humans

    K Suzuki, M Yamada, S Kurakake, N Okamura, K Yamaya, Q Liu, S Kudoh, K Kowatari, S Nakaji, K Sugawara

    EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY   81 ( 4 ) 281 - 287  2000.03  [Refereed]

     View Summary

    To investigate the mechanisms of exercise-induced immune perturbations, we measured promising immunomodulatory hormones and cytokines in plasma of 16 male marathon runners before and after a competitive 42.195-km race. Interleukin 1-beta (IL-1 beta) and interferon gamma (IFN-gamma) concentrations remained unchanged after the marathon. The cytokines IL-12, IFN-alpha and tumour necrosis factor alpha (TNF-alpha) could not be detected even using highly sensitive specific immunoassays, indicating at least that overshooting responses of these cytokines had not occurred after exercise. As mechanisms for the small changes in these cytokines, we demonstrated for the first time a significant rise in concentrations of inhibitory cytokine IL-10 in addition to the immunosuppressive hormone cortisol, although concentrations of IL-4 and transforming growth factor-beta (TGF-beta) were unaffected by the race. Furthermore, concentrations of IL-1 receptor antagonist (IL-1ra) and IL-6, which are negative-feedback inhibitors of cytokine production, increased by more than 100 times. As for humoral mediators of neutrophil mobilization, concentrations of growth hormone (GH), cortisol and granulocyte colony-stimulating factor (G-CSF) increased significantly. In addition, concentrations of neutrophil-priming substances (IL-6, IL-8, G-CSF, GH and prolactin) also increased significantly and the induction of IL-8 and G-CSF with exercise was demonstrated for the first time in the present study. In contrast, IL-2 concentration decreased, by 32%, and this was correlated with the induction of nitric oxide (NO) production. Muscle damage, monitored using changes in concentrations of creatine kinase and myoglobin, was also observed. These results suggested that exercise-induced pathogenesis including previously reported immunosuppression and neutrophil hyper-reactivity might be attributed, at least partly, to the systemic dynamics of the above bioactive substances.

  • Antioxidant activities of natural 9-cis and synthetic all-trans beta-carotene assessed by human neutrophil chemiluminescence

    Q Liu, K Suzuki, S Nakaji, K Sugawara

    NUTRITION RESEARCH   20 ( 1 ) 5 - 14  2000.01  [Refereed]

     View Summary

    Much attention has been focused on the biological activity of geometrical isomers of beta -carotene. In the present study, the in vitro antioxidant activities of a natural 9-cis beta -carotene purified from Dunaliella bardawil and synthetic all-trans beta -carotene were studied using enhanced human neutrophil chemiluminescence upon stimulation with opsonized zymosan. Lucigenin-dependent chemiluminescence was used for largely measuring superoxide anion (O-2(-)) production, luminol-dependent chemiluminescence with sodium azide (NaN3) and horseradish peroxidase (HRP) was used for measuring hydrogen peroxide (H2O2) production, and luminol-dependent chemiluminescence without oxidant metabolism modulator was used for determination of myeloperoxidase (MPO)-mediated production. With beta -carotene concentration of 1.4 mu M, neutrophils showed a decrease:in lucigenin-dependent chemiluminescence to 75% and 78% of controls (p&lt;0.05) by natural 9-cis carotene and synthetic all-trans beta -carotene, respectively. With beta -carotene concentration of 1.86 mu M, neutrophils also showed a decrease in luminol-dependent chemiluminescence in the presence of NaN3 and HRP to 86% and 81% of controls (p&lt;0.05) by natural 9-cis beta -carotene and synthetic all-trans beta -carotene, respectively. On the other hand, neutrophils did not show a statistically significant difference in any chemiluminescence assay by the same concentration of natural 9-cis beta -carotene and synthetic all-trans beta -carotene. These results suggest that, up to the concentration of 1.86 mu M, natural 9-cis beta -carotene and-synthetic all-trans beta -carotene exert equal antioxidant activities assessed by enhanced human neutrophil chemiluminescence. (C) 1999 Elsevier Science Inc.

  • Endurance exercise causes interaction among stress hormones, cytokines, neutrophil dynamics, and muscle damage

    K Suzuki, M Totsuka, S Nakaji, M Yamada, S Kudoh, Q Liu, K Sugawara, K Yamaya, K Sato

    JOURNAL OF APPLIED PHYSIOLOGY   87 ( 4 ) 1360 - 1367  1999.10  [Refereed]

     View Summary

    We analyzed adaptation mechanisms regulating systemic inflammatory response of the stressed body by using an experimental challenge of repeated exercise bouts and accompanying muscle inflammation. Eight untrained men bicycled at 90 W for 90 min, 3 days in a row. Exercise induced peripheral neutrophilia with a leftward shift of neutrophil nucleus and neutrophil priming for oxidative activity determined by luminol-dependent chemiluminescence. Plasma growth hormone and interleukin-6 rose significantly after exercise and were closely correlated with the neutrophil responses. Serum creatine kinase and myoglobin levels as muscle damage markers rose after exercise in "delayed onset" and were closely correlated with the preceding neutrophil responses. These exercise-induced responses were strongest on day I, but the magnitude gradually decreased with progressive daily exercise. In contrast, the magnitude of catecholamine responses to exercise sessions gradually rose, possibly suppressing neutrophil oxidative responses. These results indicate that stress-induced systemic release of bioactive substances may determine neutrophil mobilization and functional status, which then may affect local tissue damage of susceptible organs.

  • Distribution of heme oxygenase isoforms in rat liver - Topographic basis for carbon monoxide-mediated microvascular relaxation

    N Goda, K Suzuki, M Naito, S Takeoka, E Tsuchida, T Tametani, M Suematsu

    JOURNAL OF CLINICAL INVESTIGATION   101 ( 3 ) 604 - 612  1998.02  [Refereed]

     View Summary

    Carbon monoxide (CO) derived from heme oxygenase has recently been shown to play a role in controlling hepatobiliary function, but intrahepatic distribution of the enzyme is unknown. We examined distribution of two kinds of the heme oxygenase isoforms (HO-1 and HO-2) in rat liver immunohistochemically suing monoclonal antibodies. The results showed that distribution of the two isoforms had distinct topographic patterns: HO-1, an inducible isoform, was observed only in Kupffer cells, while HO-2, a constitutive form, distributed to parenchymal cells, but not to Kupffer cells. Both isoforms were undetectable in hepatic stellate cells and sinusoidal endothelial cells. Of the two isoforms, HO-2 in the parenchymal cell rather than HO-1 in the Kupffer cell, appears to play a major role in regulation of microvascular tone. In the perfused liver, administration of HbO(2), a CO-trapping reagent that can diffuse across the fenestrated endothelium into the space of Disse, elicited a marked sinusoidal constriction, while administration of a liposome-encapsulated Hb that cannot enter the space had no effect on the microvascular tone. These results suggest that CO evolved by HO-2 in the parenchymal cells, and released to the extrasinusoidal space, served as the physiological relaxant for hepatic sinusoids.

  • Analysis and assessment of the capacity of neutrophils to produce reactive oxygen species in a 96-well microplate format using lucigenin- and luminol-dependent chemiluminescence

    H Hasegawa, K Suzuki, S Nakaji, K Sugawara

    JOURNAL OF IMMUNOLOGICAL METHODS   210 ( 1 ) 1 - 10  1997.12  [Refereed]

     View Summary

    The chemiluminescence (CL) assay has been used to measure the reactive oxygen species (ROS)-generating capacity of phagocytes. To achieve more optimal measurement conditions for a multi-channel microplate photon-counting CL analyzer with the cooled charge-coupled device (CCD) camera which offers enhanced sensitivity, we investigated factors affecting the variability in lucigenin-dependent CL (LgCL) measurement of human neutrophil; stimulated with either opsonized zymosan (OZ) or phorbol myristate acetate (PMA). We obtained sensitive LgCL responses with good reproducibility and rapid data-acquisition using 50 mu l neutrophils (3 x 10(6) cells/ml) and 50 mu l of 0.5 mM lucigenin per well, in addition to either 100 mu l of OZ (5 mg/ml) when zymosan was opsonized with 10-20% serum or; 100 mu l of PMA solution (1 x 10(-6) M) with automatic regular intervals of mixing and detection during the continuous measurement at 37 degrees C. Furthermore, we studied the contribution of various ROS to LgCL and luminol-dependent CL (LmCL) using modulators of ROS metabolism including superoxide dismutase (SOD), catalase, deferoxamine and sodium azide (NaN3). LgCL was inhibited by SOD but not by the other agents, whereas LmCL was inhibited by NaN, and deferoxamine, Thus, it was demonstrated that LgCL detects the superoxide anion with high selectivity whereas the LmCL assay measures myeloperoxidase (MPO)-mediated formation of hypochlorous acid, Such microplate-based multiple measurements facilitate the accurate assessment of phagocytic function. (C) 1997 Elsevier Science B.V.

  • Capacity of circulating neutrophils to produce reactive oxygen species after exhaustive exercise

    K Suzuki, H Sato, T Kikuchi, T Abe, S Nakaji, K Sugawara, M Totsuka, K Sato, K Yamaya

    JOURNAL OF APPLIED PHYSIOLOGY   81 ( 3 ) 1213 - 1222  1996.09  [Refereed]

     View Summary

    To investigate the cause of disagreement within the large body of literature concerning the effect of exercise on the capacity of circulating neutrophils to produce reactive oxygen species (ROS), 10 male endurance-trained athletes underwent maximal exercise. The generation of superoxide radical (O-2(-).) by neutrophils was first detected on a cell-by-cell basis by using histochemical nitro blue tetrazolium tests performed directly on fresh unseparated blood, which showed that responsive neutrophils under several stimulatory conditions relatively decreased after exercise. Similarly, O--(2). detected with bis-N-methylacridinium nitrate (lucigenin)-dependent chemiluminescence (CL) of a fixed number of purified neutrophils on stimulation with opsonized zymosan was decreased slightly after exercise. In contrast, the 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol)dependent CL response of the neutrophils indicative of the myeloperoxidase (MPO)-mediated formation of highly reactive oxidants was significantly enhanced after exercise. It therefore suggests that the pathway of neutrophil ROS metabolism might be forwarded from the precursor O-2(-). production to the stages of more reactive oxidant formation due to the facilitation of MPO degranulation. In addition, these phenomena were closely associated with the exercise-induced mobilization of neutrophils from the marginated pool into the circulation, which was mediated by the overshooting of catecholamines during exercise. These findings indicate that the use of different techniques for detecting ROS or the different stages of neutrophil ROS metabolism could explain some of the disparate findings of the previous studies.

  • Effects of exhaustive endurance exercise and its one-week daily repetition on neutrophil count and functional status in untrained men

    K Suzuki, S Naganuma, M Totsuka, KJ Suzuki, M Mochizuki, M Shiraishi, S Nakaji, K Sugawara

    INTERNATIONAL JOURNAL OF SPORTS MEDICINE   17 ( 3 ) 205 - 212  1996.04  [Refereed]

     View Summary

    Whereas endurance exercise is known to induce marked neutrophilia, it remains to be fully understood as to whether the cell functions are altered as well as whether the adaptability of the responses to training occurs. To address both of these issues, we undertook the present longitudinal investigation in ten healthy untrained men (20-24 years, VO(2)max 39.1 +/- 4.2 ml/kg/min). The exercise protocol was 7 consecutive sessions of the same workload performed each day for 1.5 h at an intensity of 70 % of VO(2)max. Peripheral blood samples were obtained before, immediately after, and 1 h after exercise on Days 1, 4, and 7, and served for determination of total and differential leukocyte counts, chemotaxis and chemiluminescence of neutrophils. Acute endurance exercise caused marked peripheral neutrophilia with significant increase in both absolute number and relative proportion of band neutrophils (p &lt; 0.01, respectively), indicating partial recruitment of bone marrow neutrophils. While chemotaxis remained unaltered following exercise, reactive oxygen species generation of neutrophils, measured by luminol-dependent chemiluminescence upon stimulation with opsonized zymosan, was not only significantly enhanced following exercise (p &lt; 0.01), but also associated with the proportional increase in band neutrophils (r = 0.727, p &lt; 0.05), suggesting that neutrophils mobilized from the bone marrow following endurance exercise may possess higher responsiveness. On the other hand, the magnitude of the exercise-induced changes was reduced gradually by daily repeated exposure to endurance exercise, but none of the trends were significant except the decline in resting segmented neutrophil counts (p &lt; 0.05) at least during a 1-wk period of repeated exercise sessions.

  • EFFECTIVENESS OF LOWER-LEVEL VOLUNTARY EXERCISE IN DISEASE PREVENTION OF MATURE RATS .1. CARDIOVASCULAR RISK FACTOR MODIFICATION

    K SUZUKI, K MACHIDA

    EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY   71 ( 2-3 ) 240 - 244  1995.08  [Refereed]

     View Summary

    To evaluate the chronic effects of voluntary exercise at lower levels on primary cardiovascular risk factors, inactive strain male Fischer rats were housed either with or without free access to activity wheels under controlled environmental conditions. The average amount of exercise for the 35-week duration was 722 (SD 362) m . day(-1). rat(-1), which was among the lowest found in the existing reports. Nonetheless, the body mass gains of the exercising rats were markedly inhibited, being 13% less (P &lt; 0.001) than those of the sedentary controls, despite a 22% increase in food consumption (P &lt; 0.0001), suggesting a remarkable prevention of adiposity. A noticeable improvement of serum lipid profiles was also found; a 53% reduction in triglyceride concentrations (P &lt; 0.01) and a 13% increase in high density lipoprotein cholesterol concentrations (P &lt; 0.05). In addition, resting systolic blood pressure was lowered by 7% (P &lt; 0.01). These results would suggest that even lower-level physical activity, if continued regularly, will attenuate the age-related development of cardiovascular risk factors associated with a sedentary lifestyle.

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Books and Other Publications

  • 別冊「医学のあゆみ」健康寿命延伸に寄与する体力医学

    鈴木政登編( Part: Contributor, 自然免疫・炎症に及ぼす運動の影響―そのメカニズム)

    医歯薬出版  2020

  • Concurrent Aerobic and Strength Training: Scientific Basics and Practical Applications

    Schumann, M, Rønnestad, B編(Effects of endurance-, strength-, and concurrent training on cytokines and inflammation)

    Springer  2019.11

  • 管理栄養士講座『改定 感染と生体防御』

    酒井徹, 鈴木克彦編

    建帛社  2018.09 ISBN: 9784767906324

  • アスリートの栄養アセスメント

    早稲田大学スポーツ栄養研究所, 田口素子編( Part: Contributor, 「心拍と血圧」「尿検査」「内科的所見」)

    第一出版  2017.12 ISBN: 9784804113746

  • 『ニュー運動生理学Ⅱ』 鈴木克彦「サイトカイン」(分担執筆)

    宮村実晴編集

    真興交易医書出版部  2015.01

  • 『健康運動指導士養成講習会テキスト』 鈴木克彦「運動と免疫能」(分担執筆)

    公益財団法人, 健康, 体力づくり事業財団編

    南江堂  2014

  • 『ランニング医学大辞典』 鈴木克彦「28章 感染症」(分担執筆)

    福林徹, 渡邊好博監訳

    西村書店(http://www.nishimurashoten.co.jp/pub/details/114_436.html)  2013.09

  • 『保健体育科教育のための生理学と運動生理学』 鈴木克彦「運動によって変化する免疫系の機能と適応」(分担執筆)

    村岡功編

    市村出版(http://www.ichimura-pub.com/2012/book0042.html)  2013

  • 『運動科学の基礎 アスリートのパフォーマンス向上のために』 鈴木克彦「運動と環境」(分担和訳)

    足立和隆監訳

    西村書店  2012

  • Suzuki K. Cytokines. in 『Encyclopedia of Exercise Medicine in Health and Disease』

    Mooren FC, eds

    Springer(http://www.springer.com/medicine/book/978-3-540-36065-0)  2012 ISBN: 9783540360650

  • 『スポーツ現場に生かす運動生理・生化学』 鈴木克彦「スポーツ選手の体調管理と免疫機能」(分担執筆)

    樋口満

    市村出版  2011

  • 『教養としてのスポーツ科学』 鈴木克彦「身体運動と免疫」(分担執筆)

    早稲田大学スポーツ科学学術院編

    大修館書店  2011

  • 『身体トレーニング 運動生理学からみた身体機能の維持・向上』 鈴木克彦「サイトカイン」(分担執筆)

    宮村実晴

    真興交易医書出版部  2009.03

  • Fluid osmolarity of sports drinks. Food and Beverage Consumption and Health(分担執筆)

    Ishijima T, Suzuki K, Higuchi M

    Nova publishers  2009

  • 『予防としてのスポーツ医学』 椎葉大輔、鈴木克彦「花粉症・運動誘発性アナフィラキシー」(分担執筆)

    臨床スポーツ医学, 臨時増刊号

    文光堂  2008.11

  • 『「健康福祉」人間科学』 鈴木克彦「生活習慣病の予防・治療における運動の役割」(分担執筆)

    中島義明, 木村一郎

    朝倉書店  2008.05

  • 『在宅ケア事典』 鈴木克彦「腎・内分泌・代謝系疾患と在宅ケア」(分担執筆)

    日本在宅ケア学会監修

    中央法規出版  2007.12

  • 『健康運動指導士養成講習会テキスト』 鈴木克彦「運動と免疫能」(分担執筆)

    財団法人健康, 体力づくり事業財団編

    社会保険研究所  2007.04

  • 『体力とは何か—運動処方のその前に—』 鈴木克彦「運動不足と体力」(分担執筆)

    長澤純一編

    ナップ  2007.03

  • 『生命・医療・福祉ハンドブック』 鈴木克彦「遺伝子解析と予防医学」、「運動と健康」、「生活習慣病予防と代替医療」、「免疫」、「抗体」(分担執筆)

    内山明彦編

    コロナ社  2007.01

  • 『管理栄養士講座 感染と生体防御』 鈴木克彦「運動と生体防御」(分担執筆)

    山本茂, 森口覚, 酒井徹編

    建帛社  2004.04

  • 『スポーツ医学検査測定ハンドブック』 鈴木克彦「サイトカイン」、「アレルギー」、「インフルエンザ、SARS」(分担執筆)

    臨床スポーツ医学, 臨時増刊号

    文光堂  2004

  • 『環境化学物質の代謝』 鈴木克彦、菅原和夫「重金属」(分担執筆)

    荻野景規編

    財団法人日本公衆衛生協会  2003.12

  • 『新運動生理学』 鈴木克彦「好中球と炎症性サイトカイン」(分担執筆)

    宮村実晴編

    真興交易医書出版  2001.10

  • 新運動生理学

    宮村実晴編( Part: Contributor, 好中球と炎症性サイトカイン)

    真興交易医書出版部  2001 ISBN: 4880036692

  • 活性酸素と運動

    大野秀樹, 跡見順子, 伏木 亨 編( Part: Contributor, 「骨格筋への影響は?」「絶対安静・運動不足の影響は?」)

    杏林書院  1998.10 ISBN: 4764410389

  • 『活性酸素と運動』 鈴木克彦、菅原和夫「骨格筋への影響は?」、「絶対安静・運動不足の影響は?」(分担執筆)

    大野秀樹, 跡見順子, 伏木亨編

    杏林書院  1998.10

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Misc

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Industrial Property Rights

  • 好中球機能検査システムおよび好中球機能検査方法

    4869020

    鈴木 克彦, 佐藤 裕子

    Patent

Works

  • Sabbatical period in Australia

    2013.09
    -
    2014.09

  • トライアスロン競技選手の生体負担の評価とストレス制御に関する調査、医科学サポート

    Other 

    2007.09
    -
     

  • 東京都健康長寿医療センター研究所主催の高齢者疫学調査における健診医担当

    Other 

    2006.05
    -
     

Other

  • I have bee...

     View Summary

    I have been involved in researches on setting of basic measurement conditions of immune functions, assessment of the effects of micronutrients and drugs on phagocyte functions, and investigation of mechanisms and regulation of inflammatory disorders. Especially, a patent on neutrophil functional assessment and applications was opended in 2011 (http://jstore.jst.go.jp/seedsDetail.html?seeds_id=13505).

Awards

  • Young Investigators Award, International Society of Exercise and Immunology

    2001.07  

Research Projects

  • 運動により分泌されるエクソソームの機能解析と生理的役割の解明

    Project Year :

    2020.10
    -
    2024.03
     

     View Summary

    申請者らが確立した生体分子の網羅的解析法を改良することで、分泌エクソソームを同定するとともに、国際共同研究によって、エクソソームによる酸化ストレスおよび脳神経系機能の制御という観点から、運動による脳機能の向上の分子メカニズムの解明を行う。具体的には、定期的な運動により変動する分泌エクソソームを探索し、運動による酸化ストレスや生体機能の変動を制御する分泌エクソソームを同定するため、最先端のエクソソーム機能解析技術を用いて、運動トレーニングにより変動する分泌クソソームの探索を行い、運動による酸化ストレスや脳神経系機能の変動を制御する分泌エクソソームを同定する

  • 骨格筋由来の生理活性因子による運動効果の機序究明とバイオマーカーの開発

    Project Year :

    2020.04
    -
    2024.03
     

     View Summary

    近年、エクソソームとよばれる顆粒がさまざまな生理作用を有することが明らかにされた。本研究では、血液や骨格筋の培養液などからエクソソームを単離・回収し、細胞内代謝環境の変化や筋収縮刺激に伴うエクソソームの量的・質的変動を明らかにし、運動に伴う骨格筋由来の生理活性因子による生体調節機構に新たな知見を得る。また、運動に伴うエクソソームの変動を検証し、運動やストレスを反映する新規バイオマーカーとしての妥当性を検討する

  • 身体と口、喉の渇きの時間的、定量的な関係解明

    Project Year :

    2020.04
    -
    2023.03
     

     View Summary

    本研究では、スポーツ中に刻々変化する体の渇きを直接計測することが難しい現状を踏まえて、口腔の唾液や粘膜湿潤度を代替モニタリングすることで、その変動を精度よく検出することができるか否かを明らかにすることである。そこでまず体の渇きと喉、口の渇きの3者間の時間的、定量的の関係解明に取り組み、最適な口内指標の決定を試みる。その上で、実際のスポーツシーンでの検証作業を行う予定である。本研究成果に基づき、スポーツ選手の体の渇きを非侵襲的かつ簡便にモニタリングできるツールやセンサデバイスの開発に繋げ、コンディションの維持やパフォーマンスの最適発揮、脱水・熱中症の事故防止に貢献することが期待される

  • ケト適応状態における外因性ケトン体投与に対する運動能力と代謝への影響

    Project Year :

    2020.04
    -
    2022.03
     

     View Summary

    A ketogenic diet (KD) is a special high-fat diet that contains minimum carbohydrate and could induce nutritional ketosis. Over time, the body will acclimate to use ketone bodies as a primary fuel to achieve keto-adaptation. We aim to apply exogeneous ketone bodies use on keto-adapted subjects

  • 伸張性運動に伴って生ずる筋損傷に対する非侵襲的なバイオマーカーの妥当性

    Project Year :

    2019.04
    -
    2022.03
     

     View Summary

    激しい運動は時に筋損傷(EIMD)を誘発する。多くの先行研究では血中クレアチンキナーゼ(CK)を利用してきた。一方、近年骨格筋を構成する蛋白質であるタイチンのN-末端断片(TNF)が、EIMDに伴い尿中に遊離し、CKと同様の濃度変化を示すことが報告され、尿をEIMDの指標として使用できる可能性が示唆された。本研究は、運動に伴うEIMD評価におけるTNFの妥当性を検討するために、Ⅰ性別、年齢、運動経験が異なる者を対象にTNFの変化、およびⅡEIMDが発症しない持久性運動後のTNFの変化を観察することで特性の異なる被験者への応用や運動様式の違いによるTNFの変動動態を明らかにしていく

  • 筋萎縮進行におけるプロスタノイドの質量分析イメージング解析と治療標的分子の同定

    Project Year :

    2017.04
    -
    2020.03
     

     View Summary

    本研究課題では、不動性筋萎縮モデルとして尾部懸垂を用い、筋萎縮におけるプロスタノイドの産生動態をLC-MS 質量分析による定量、質量分析顕微鏡を用いた質量分析イメージングにより観察し、プロスタノイドが筋萎縮の主因となるか否かを検討した。これまでに、プロスタノイドの検出から、その産生阻害剤の効果検証を段階的に進め、演繹的な実験計画により分子標的の同定を試みてきた。今年度は、その効果検証として、尾部懸垂マウスにおけるプロスタノイド阻害剤の投与効果の検討を行った。前年度までに得られた筋萎縮におけるプロスタノイド産生の結果をもとに、プロスタノイドの産生阻害剤をマウスに投与し、筋萎縮の抑制効果を判定した。これら実験の結果、プロスタノイド産生阻害剤の投与マウスでは筋萎縮の改善効果が認められた。さらに、筋組織におけるプロスタノイドの発現解析により、尾部懸垂により萎縮する筋組織におけるプロスタノイド産生細胞の動態解析を行った。既存法であるIn vivo 発光イメージャー、3次元マイクロCT 解析を併せて行った結果、筋萎縮におけるプロスタノイド産生細胞に関わる候補細胞を解析した。これらは炎症性疾患に関わるいくつかの免疫細胞などで構成されていることが明らかとなった。さらに、産生細胞を調べたところ、血球系細胞であるマクロファージ系の細胞によるプロスタノイド産生が筋萎縮に関与することが示唆された。細胞培養実験を併せて行った結果、マクロファージではプロスタノイド合成酵素の産生が亢進していた。本研究成果は、将来の廃用性筋萎縮やサルコペニア、広くはロコモティブシンドロームの治療につながる分子標的としてのプロスタノイド産生阻害剤の開発のみならず、SiRNA 導入、受容体拮抗阻害薬、天然由来機能性因子を活用し、不動性筋萎縮の新規な治療薬や治療因子の開発につなげてゆきたい。令和元年度が最終年度であるため、記入しない。令和元年度が最終年度であるため、記入しない

  • Development of novel biomarkers on exercise, stress and aging and their functional analyses

    Project Year :

    2015.04
    -
    2019.03
     

     View Summary

    Using aging-model mice and young mice together with acute exercise model mice and endurance exercise model mice, grobal mRNA sequence and ribosome profiling were performed using next-generation sequencer. Changed variables and factors based on the analyses were further confirmed by real-time PCR and western blotting, and messages and proteins were quantified. In addition, gene network analyses were performed to capture changes at the system level of skeletal muscle, and found interesting genes and their potentially new functions

  • Grobal analyses of translational dynamics in exercise-induced inflammation

    Project Year :

    2014.04
    -
    2016.03
     

     View Summary

    Not only the dynamic changes in transcription, but also those in translation in relation with mechanisms of action have great impacts on biological systems. In addition, recently, technological advancement due to ribosome profiling using the next-generation sequencer ennables us to analyze translational dynamics globally and with a high degree of accuracy for one nucreotide unit. Using this technology, we investigated the effect of exercise on translational dynamics with a genome-wide, and revealed new mechanisms of action together with nobel cis-elements in response to acute pro- and anti-inflammatory responses

  • Development of novel biomarkers on exercise, stress and aging

    Project Year :

    2011.04
    -
    2015.03
     

     View Summary

    In order to explore new biomarkers which respond to exercise and stress, we performed proteomics using blood and urine samples before and after exhaustive exercise and identified the molecules using mass spectrometric analyses. Among the molecules which exhibited remarkable increases, we developed measurement system using ELISA. Also, we identified several microRNAs in the blood circulation in response to acute resistant exercise using microarray, and quantified expression levels using real-time PCR. Furthermore, we set up global analyses of translation dynamics using ribosome profiling for biomarker discovery

  • Effects of immune cells on inflammatory responses and oxidative stress in the exercise-induced muscle damage

    Project Year :

    2012.04
    -
    2014.03
     

     View Summary

    Although exercise induces muscle damage, the underlying mechanisms are not fully understand. The present study examined the involvement of neutrophils and macrophages in the exhaustive exercise-induced acute muscle damage in mice by application of the inhibitory neutrophil migration model and macrophage depletion model. The administlation of anti-neutrophil antibody inhibited the infiltration of Ly-6G positive cells (neutrophils) in the gastrocnemius, and the administration of clodronate liposome reduced the infiltration of F4/80 positive cells (macrophages) and related inflammatory reactions. Therefore, it was demonstrated that neutrophils and macrophages are involved in the exercise-induced muscle damage

  • Effect of Skeletal Muscle-Drived Growth-Arrest Specific gene 6 on Phenotype Switching of Macrophages in Injured-Muscle in mice

    Project Year :

    2010.04
    -
    2013.03
     

     View Summary

    This study examined that the Gas6 expression and regulation of macrophages functions by Gas6 in injured-skeletal muscle. In the results, it observed that injured-skeletal muscle by Cardiotoxin-induction was increased Gas6 expression compared with control muscle. In addition, it observed that Gas6 stimulation regulate macrophages phenotype

  • Effectiveness and safety of hot bathing utility on health promotion

    Project Year :

    2009
    -
    2011
     

  • Preclinical evaluation of H12(ADP) liposome as artificial platelets (Effectiveness and safety)

    Project Year :

    2009
    -
    2011
     

  • Functional assessments of extracts from vegetables

    Project Year :

    2008
    -
     
     

  • Studies on complementary and alternative medicine in cancer

    Project Year :

    2008
    -
     
     

  • Development of the personalized health promotion program for the prevention of life-style related disease : Contribution of Cardiorespiratory Fitness and Obesity Genes to the Risk Factors of Metabolic Syndrome

    Project Year :

    2006
    -
    2007
     

     View Summary

    Little information is available regarding the relationships among human obesity-related genes, cardiorespiratory fitness and metabolic syndrome (MS) or predisposition to MS (MP). The purpose of this study was to investigate the contribution of obese-related genes and cardiorespiratory fitness to the MS in middle-aged and old men and women. Seven hundred fifty eight healthy men and women participated in this study. We measured maximal oxygen uptake (VO2max) during an incremental cycle ergometer exercise test. Serum BDL-cholesterol, triglyceride and plasma glucose levels were measured in all subjects. Total and regional lean soft tissue and fat mass were also measured by dual-energy X-ray absorptiometry (DXA). We analysed the genotype of b3-adrenergic receptor (ADRB3 64 T>A), peroxisome proliferator-activated receptor gamma (PPARG 1431 C>. Pro12Ala), uncoupling protein 1(UCP1 3826 A>G), and fatty acid-binding protein 2(FABP2 54 A>T) using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Significant associations were observed between VO2max less than 32 nil/kg/min in men and 30 ml/kg/min in women and increases in the number of risk factors of MS (P<0.05). Poor VO2max was associated with the prevalence of MS in men and women independently of the 1431 C>T polymorphism in PPAR_Y. It is important to maintain high cardiorespiratory fitness regardless of the obesity gene variance for the prevention of metabolic syndrome.

  • Physiological responses to short-term, high-intensity intermittent rowing exercise

    Project Year :

    2006
    -
    2007
     

     View Summary

    This study was designed to observe physiological response during one bout of high-intensity intermittent short time rowing exercise and to evaluate the effect of repeated bouts of the exercise on rowing performance for college oarsmen.
    The high-intensity, intermittent exercise consisted of either five or eight bouts of 20-sec rowing with the maximal effort of the subjects, and each bout was separated by 10-sec rest. In the first experiment, subjects reached to the maximal level of VO_2 and heart rate at the fourth set during both 5- and 8- sets of high-intensity, intermittent exercises. However, blood lactate concentration after high-intensity, intermittent exercise was significantly lower in the 5-set protocol than in the 8-set protocol (9.0±1.2vs. 11.3±1.6mM, p<0.05), suggesting that the 8-set protocol is much vigorous and better than the 5-set protocol as high-intensity, intermittent rowing exercise for rowers. In the 6-week rowing training, low frequency group (LF: 2 times/week) markedly improved their rowing performance (Δ3.2±1.9sec/1000m, Δ8.4±5.5sec/2000m time trials), whereas high frequency group (HF: 5 times/week) improved only in 1000m trial (Δ4.5±2.3sec).
    Bowers performed 5-week training regimens by single scull boat on water which consisted of high-intensity rowing exercise(8 sets/bout, 2 times/week). Their rowing performance in the 2000m-time trial on ergometer was significantly improved.
    These results suggest that high-intensity, intermittent rowing exercise on both ergometer and on water, which consists of 20-sec maximal exertion with 10-sec interval, 8 sets/bout, 2 times/week, more than 6 weeks, could be recommended to the collegiate rowers in order to improve their rowing performance.

  • Research on the exercise and immunity with the University of Queensland

    Project Year :

    2005
    -
     
     

  • Development of a new method for neutrophil functional analysis

    Project Year :

    2005
    -
     
     

  • Changes in cytokines and inflammatory markers following endurance exercise and training and controls by carbohydrate drinks and supplements

    Project Year :

    2005
    -
     
     

  • スポーツ選手の免疫学的コンディショニングの必要性についての調査

     View Summary

    運動やストレスに伴う免疫学的パラメータの変化は多数報告されているものの、その意義は未だ明らかにならず、実用的な研究が少ない。そこで2003年7月国際運動免疫学会コペンハーゲン大会の将来構想委員会において、今後スポーツ選手など運動に伴う免疫学的な問題に焦点を当てることを提案した。その結果、2005年モナコでの学術集会、2007年10月日本での学術集会では実用的な研究に焦点を当てることになり、本研究の代表者永富は国際運動免疫学会副会長に選出され、同時に2005年学術集会のプログラム委員長を務めることになった。この決定に伴い、これまでほとんど行われてこなかったスポーツの現場での免疫系が関連する問題点の調査を実施した。様々なレベルのスポーツ種目指導者に面接の上、質問紙を作成し、平成15年12月〜2月にかけて、大学生、プロスポーツ選手、実業団スポーツ選手、目本代表候補選手などを対象に無記名のアンケート調査を実施し1409名から回答を得た。有効街回答1367件を分析した結果、およそ20%にあたる274名が免疫に関連する疾患がスポーツ活動の障害になっていることを申告した。原因疾患として48.9%が急性上気道感染症を、25.2%がインフルエンザを、22.3%が花粉症を挙げた。インフルエンザ以外は軽症疾患といえどもスポーツ実施者にとっては無視できない問題であることがあらためて浮き彫りにされた。詳細な分析結果は現在報告書にまとめつつあり、各競技団体に配布する予定である。また本研究代表者が代表を務める日本運動免疫学研究会では2005年、2007年の学会に向けて今回明らかになった現場での問題点に関連する研究を奨励していく予定である

  • Analysis and assessment of the capacity of neutrophils to produce reactive oxygen species by application of flow cytometry.

     View Summary

    First, in order to advance the various researches in laboratory animals, we examined fundamental measurement conditions for detecting reactive oxygen species of rat peripheral neutrophils by use of flow cytometry. For measuring phagocytic activity with flow cytometry, Staphylococcus aureus was labeled with fluoresce in isothiocynate (FITC) and used as phagocytic particles. We decided the optimal concentrations of FITC in regard to the fluorescent intensity. As for the measurement of reactive oxygen-producing activity, hydroethidine (HE) which reacts with superoxide was used and the suitable concentration was determined by dependency of the fluorescent intensity. Furthermore, simultaneous measurement of both of these activities was made possible by detecting FITC and HE at the different wave length. In addition, nitroblue tetrazolium (NBT) reduction method was also performed using the same samples. As a result, strong correlations were confirmed between both measurement methods.Next, we investigated the fundamental measurement conditions in case of human peripheral blood. It was found that hemolysis procedure after the cellular reaction could affect flow cytometry distribution of neutrophils, lymphocytes and monocytes, and we improved the operation methods. On the other hand, isolated neutrophils lose activity soon, and there was a necessity to measure reactive oxygen production soon after the separation from the whole blood. Then we tried to examine the high sensitivity detection probe by comparing HE with Dihydrorhodamine (DHR) -123, Dichrolo Fluorescein Diacetate (DCFH-DA). DHR-123 was superior in sensitivity, by coexisting NaN3 which is the inhibitor of myeloperoxidase, compared with DCFH-DA. Concerning the phagocytic particles, FITC-labeled Staphylococcus aureus and zymosan were used, but the culture of pathogens is dangerous and not easy to handle to label with FITC. In addition, zymosan caused aggregation of neutrophils and was not suitable for the measurement of flow cytometry method. For those reasons, Carboxylate Microspheres of the diameter 1 μ m with fluorescence dye was used, and it became possible to measure phagocytic activity without such difficulties. We could set up the detection wave length of Carboxylate Microspheres without interference of those of HE and DHR-123, and simultaneous analyses of phagocytosis and reactive oxygen production became possible.Furthermore, by applying the principle of flow cytometry that can measure several indices simultaneously by changing detection wave length, the simultaneous measurement of various molecules using human plasma samples became possible. Conventional enzyme-linked immunosorbent assay (ELISA) takes larger sample volume and takes time for the measurement. On the other hand, this method have greater range of measurement, but the sensitivity was poorer than ELISA method. Taken together, flow cytometry method is enable us to measure multi-channel parameters that we could not do until recently

  • 免疫能、炎症、老化に関する制御機構の解析と運動・栄養による予防医学的介入研究

     View Summary

    前年度に引き続き、免疫能、炎症、老化に関する機序の解析と運動・栄養による予防医学的介入研究を進めた。前年度は極端な激運動について検討を行ったが、本年度は運動処方を念頭に置いた健康増進運動についても検討を進めた。健常若年者を対象とした歩行運動の介入研究(1日30分、週6日、3週間)では、トレーニングによって白血球・リンパ球サブセット、NK細胞活性、Tリンパ球幼弱化反応に有意な変化は認められなかったが、血漿インターロイキン12p40濃度に有意な減少が認められ、細胞性免疫が活性化されやすい状態になる可能性が示唆された。高齢者を対象とした12週間の低強度筋力トレーニングでは、CRP、SSA、HSP70に有意な低下が認められ、筋肥厚とCRP、TNF-αの変化率に逆相関が認められたことから炎症の軽減が筋肥大にも関わる可能性が示唆された。前立腺癌がん患者を対象とした筋力トレーニングの介入研究では、インスリン濃度が低下した以外にはホルモン、サイトカインに顕著な影響は認められなかったものの、筋力改善によるADLの改善効果が示され、運動トレーニングががん患者においても有用であることを示した。なお、運動・トレーニングの影響を評価するためのバイオマーカーの検索も試みたが、現状では実用化に値するような研究結果を得ることはできなかっだ。今後も引き続き研究を行う予定である

  • 白血球機能解析を応用した生体のストレス応答と筋損傷のメカニズムの解明

     View Summary

    遅発性筋肉痛は、伸張性筋収縮などの運動後数時間から1日程度経過後より発現し、2、3日後にピークとなり1週間程度で消失するが、その機序は明らかではない。運動により白血球(好中球)の活性酸素産生能と抗酸化システムのバランスが崩れると、生体に傷害を与え、サイトカイン産生や筋組織に好中球の侵入が起きるため、筋肉痛の原因として損傷・炎症説や活性酸素による傷害説が提唱されている。そこで、健康な男性8人に片足カーフレイズ運動を負荷し、運動負荷前、直後、1日後、2日後、3日後、4日後に筋肉痛の評価と下腿周径囲を測定し、採血を行った。全血より好中球が選択的に侵入する熱可逆ハイドロゲルを用いて遊走能を測定し、ルミノール依存性化学発光により活性酸素産生能を測定した。さらに血液中の筋損傷マーカーと炎症関連物質の測定を行い、筋肉痛との関連性を検討した。その結果、運動負荷1日後から筋肉痛が発現し2、3日後がピークとなり、4日後には痛みが減少した。好中球の活性酸素産生能は運動直後に上昇し、4日後には運動前と比較して有意に低下した。筋損傷マーカーのGOTは有意に上昇し、CK、ミオグロビンは上昇傾向を示した。サイトカインは抗炎症性サイトカインのIL-10のみ運動直後に有意に上昇し、GOT、CKとの間に有意な負の相関が認められた。激運動や持久性運動では好中球数が増加し活性酸素産生能が亢進する一方、生体には運動により発生する活性酸素を消去する抗酸化システムが存在する。先行研究では運動後に得られた血漿に活性酸素消去能の亢進が示されたが、本研究でもそのために好中球活性酸素産生能の抑制が生じたか、IL-10が好中球機能を抑制した可能性が考えられた

  • Pathogenesis and site specificity of delayed onset muscle soreness : toward training modality effective for strength gain while minimizing muscle soreness

     View Summary

    This study investigated the relationships between the degree of muscle fatigue and changes in the delayed onset muscle soreness(DOMS), biochemical markers of muscle damage and joint performance, induced by calf-raise exercises at two different frequencies. The results suggest that 1) muscle fatigue is less by the faster exercise, 2) the degree of DOMS and the amount of muscle damage markers are related with the degree of muscle fatigue and muscle-specific, but joint performance rapidly recovers after fatigue regardless of soreness or retained biochemical changes, and that 3) fascicle and tendon behavior during exercise is altered in relation to the observed changes

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Presentations

  • コロナ渦から学ぶ健康管理とコンディショニング

    赤間 高雄, 絹笠 友則, 長尾 雅史, 渡部 厚一, 鈴木 克彦  [Invited]

    第76回日本体力医学会大会 

    Presentation date: 2021.09

    Event date:
    2021.09
     
     
  • Involvement of neutrophils in exercise-induced muscle damage and its modulation

    Katsuhiko SUZUKI  [Invited]

    International Conference on Food Technology & Nutrition  (Hampton by Hilton Paris Clichy, Paris)  PrideConferences

    Presentation date: 2021.09

    Event date:
    2021.09
     
     
  • Strengthening Immunity Against COVID-19

    NHK

    Presentation date: 2020.06

  • Involvement of neutrophils in exercise-induced muscle damage and its modulation

    Katsuhiko Suzuki  [Invited]

    Presentation date: 2019.07

    Event date:
    2019.07
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  • The 6th International Conference on Exercise and Health

    Katsuhiko SUZUKI

    The 6th International Conference on Exercise and Health 

    Presentation date: 2019.06

    Event date:
    2019.06
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  • 超高齢社会におけるスポーツ医科学の役割-メタボとロコモの予防-

    岡浩一朗, 伊藤智子, 渡辺尚彦, 鈴木克彦  [Invited]

    第6回日本介護予防・健康づくり学会  (早稲田大学 東伏見キャンパス)  日本介護予防・健康づくり学会

    Presentation date: 2018.11

    Event date:
    2018.11
     
     
  • Involvement of neutrophils in the exercise-induced oxidative stress

    Katsuhiko Suzuki  [Invited]

    The 5 th Sports & Oxidative stress・Anti-oxidant Seminar  (Tokyo) 

    Presentation date: 2018.09

  • Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

    Katsuhiko Suzuki  [Invited]

    The 5th International Conference on Exercise and Health  (Guangzhou)  Guangzhou Sport University

    Presentation date: 2018.06

  • Cytokine response to exercise and its modulation

    Katsuhiko Suzuki  [Invited]

    13th International Society of Exercise and Immunology Symposium  (Coimbra)  International Society of Exercise and Immunology

    Presentation date: 2017.07

  • 激運動による急性腎傷害:好中球・サイトカインを中心とした炎症反応に関する基礎研究の知見

    鈴木克彦

    第7回 日本腎臓リハビリテーション学会学術集会  (つくば国際会議場)  日本腎臓リハビリテーション学会

    Presentation date: 2017.02

    Event date:
    2017.02
     
     
  • 新規白血球機能測定法の開発と運動・食品機能性評価への応用

    鈴木克彦

    第6回化学フェスタ2016 産総研特別企画:健康・スポーツ工学の発展を加速する機能材料  (タワーホール船堀)  公益社団法人日本化学会

    Presentation date: 2016.11

    Event date:
    2016.11
     
     
  • Cytokine response to exercise

    Katsuhiko Suzuki  [Invited]

    SSCYI 2016  (Budapest)  University of Physical Education

    Presentation date: 2016.07

  • 激運動によるサイトカインの動態と病態生理学的意義

    鈴木克彦

    第25回 内毒素・LPS研究会  (慶応義塾大学日吉キャンパス)  順天堂大学大学院医学研究科

    Presentation date: 2016.06

    Event date:
    2016.06
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  • 運動による全身性炎症反応とその予防

    第41回関東腎研究会 

    Presentation date: 2016.01

  • 運動誘発性筋損傷とその予防に寄与する機能性食品の探索

    鈴木克彦

    産業技術総合研究所 

    Presentation date: 2015.12

  • Neutrophil functional changes following exercise and the biological significance

    Katsuhiko Suzuki  [Invited]

    The International Colloquium of Exercise Immunology  (Korea)  Chungnam National University

    Presentation date: 2015.11

  • 運動の臓器連関と未病対策

    第22回日本未病システム学会学術総会 

    Presentation date: 2015.10

  • Exercise-induced leukocyte functional augmentation and its modulation with antioxidants

    Suzuki K  [Invited]

    11th Symposium of the International Society of Exercise and Immunology  (Vienna, Austria)  International Society of Exercise and Immunology

    Presentation date: 2015.07

  • 鈴木克彦「運動とサイトカイン」

    国立障害者リハビリテーションセンター研究所セミナー 

    Presentation date: 2015.04

  • Assessment of neutrophil functional activity following prolonged endurance exercise.

    Suzuki K, Sugama K, Yoshitani Y, Shiraishi K, Kometani T, Miura S, Yoshioka H, Mori Y

    11th Symposium of the International Society of Exercise and Immunology  (Newcastle)  International Society of Exercise and Immunology

    Presentation date: 2013.09

  • 鈴木克彦「運動誘発性筋損傷とその予防に寄与する機能性食品の探索」

    北海道大学保健科学セミナー 

    Presentation date: 2013.07

  • 鈴木克彦「新規好中球機能検査法の開発と応用」

    日本運動免疫学研究会 第2回スプリングセミナー 

    Presentation date: 2013.02

  • 鈴木克彦 「運動と免疫機能」

    日本食品免疫学会 第8回学術大会 

    Presentation date: 2012.10

  • Fluid intake and cytokine response during exercise.

    Suzuki K

    Mini Symposium of GCOE Program “Effects of exercise and dietary treatment on the regulation of physical function -Physiological and biochemical approach-”, Waseda University, Tokorozawa, Japan 

    Presentation date: 2012.01

  • Suzuki K, et al. The effects of sports drink composition on fluid intake and immunoendocrine responses to cycling in hot conditions.

    Katsuhiko Suzuki  [Invited]

    11th International Symposium of Human & Sport Science  (Daegu)  Keimyung University

    Presentation date: 2011.12

  • The comparison of circulating cytokine responses depending on exercise intensity and their biological and pathological significance.

    Suzuki K

    10th Symposium of the International Society of Exercise and Immunology  (Oxford)  International Society of Exercise and Immunology

    Presentation date: 2011.07

  • Suzuki K. Exercise and cytokines.

    Suzuki K  [Invited]

    2010 International Conference of Exercise Physiology, Korean Society of Exercise Physiology  (Soul)  Korean Society of Exercise Physiology

    Presentation date: 2010.11

  • 免疫機能の立場から

    第65回日本体力医学会大会 

    Presentation date: 2010.09

  • Suzuki K, et al. Urinary determination of cytokines in response to endurance exercise.

    Suzuki K

    9th International Society of Exercise and Immunology Symposium  (Tübingen)  International Society of Exercise and Immunology

    Presentation date: 2009.09

  • Age-related sarcopenia and low-grade inflammation controlled by exercise training.

    Suzuki K  [Invited]

    Eurasia-Pacific Uninet International Conference. Multifacetal Aspects of Aging and Aging Disease.  (Beijing) 

    Presentation date: 2008.10

  • サイトカインの変動

    鈴木克彦

    第63回日本体力医学会大会 (シンポジウム 運動・身体活動の指標としての免疫) 

    Presentation date: 2008.09

  • Hypotonic drink supplementation attenuates systemic inflammatory responses to endurance exercise in the heat.

    Suzuki K

    10th Asian Federation of Sports Medicine  (Pattaya)  Asian Federation of Sports Medicine

    Presentation date: 2007.10

  • Cytokine response to exercise.

    Suzuki K  [Invited]

    8th International Society of Exercise and Immunology Symposium  (Sendai)  International Society of Exercise and Immunology Symposium

    Presentation date: 2007.10

  • 鈴木克彦.運動時の免疫変動と制御.

    第31回 岡山スポ-ツ医科学研究会, 特別講演 

    Presentation date: 2007.09

  • 鈴木克彦.生活習慣病に対する運動の効用—概論—(兼座長)

    第62回日本体力医学会大会 (シンポジウム 生活習慣病に対する運動の効用—そのメカニズムを分子レベルで紐解く—) 

    Presentation date: 2007.09

  • 鈴木克彦.運動時の免疫変動と栄養素の補給.

    第15回 スポーツ栄養学研究会, 招待講演 

    Presentation date: 2007.06

  • 鈴木克彦.サイトカイン・代謝応答.

    第61回日本体力医学会大会 (シンポジウム 運動に対する生体のストレス応答と適応機構の解析—体力医学への応用—) 

    Presentation date: 2006.09

  • Effects of carbohydrate and fluid intake on leucocyte and cytokine responses to endurance exercise in the heat.

    Suzuki K  [Invited]

    11th annual Congress of the European College of Sport Science (ECSS). JSPFSM Exchange Symposium  European College of Sport Science

    Presentation date: 2006.07

  • Analysis of leucocyte and cytokine responses to physical stress and its possible and practical application.

    Suzuki K  [Invited]

    Joint Symposium of University of Bonn and Waseda University  (Bonn)  University of Bonn

    Presentation date: 2006.07

  • Urinary determination of cytokines for the analysis of inflammatory state in eccentric exercise-induced muscle damage.

    Suzuki K

    XXIX FIMS World Congress of Sports Medicine  (Beijing)  FIMS

    Presentation date: 2006.06

  • 鈴木克彦.Tutorial:免疫学基礎知識(兼 座長)

    第60回日本体力医学会大会ワークショップ「運動免疫学最前線」 

    Presentation date: 2005.09

  • Effects of exercise intensity and downhill running on plasma cytokine concentrations in humans.

    Suzuki K

    5th Asian Federation of Sports Medicine Congress  (Tokyo)  Asian Federation of Sports Medicine

    Presentation date: 2005.05

  • Systemic regulation of immune changes following exercise.

    Suzuki K  [Invited]

    5th International Symposium of Human & Sport Science  (Daegu)  Keimyung University.

    Presentation date: 2005.02

  • 鈴木克彦.一過性運動時の酸化ストレスにおける免疫系の関与.

    第15回ビタミンE研究会(ミニシンポジウム 運動と酸化ストレス・ビタミンE Update 2004) 

    Presentation date: 2004.01

  • Basic knowledge of cytokines, their measurement systems and the acute exercise effects.

    Suzuki K  [Invited]

    2003 Symposium of Human & Sport Science  (Daegu)  Keimyung University

    Presentation date: 2003.12

  • Effects of exhaustive exercise on circulating levels of interleukin-12 p40/p70.

    Suzuki K  [Invited]

    8th Annual Congress of the European College of Sport Science (ECSS). JSPFSM Exchange Symposium  (Salzburg)  ECSS

    Presentation date: 2003.07

  • Impact of a competitive marathon race on antioxidant defense system.

    Suzuki K

    12th International Biochemistry of Exercise Conference  (Maastricht) 

    Presentation date: 2003.07

  • Characterization of systemic cytokine release following exhaustive exercise.

    Suzuki K

    6th International Society of Exercise and Immunology Symposium  (Copenhagen)  International Society of Exercise and Immunology Symposium

    Presentation date: 2003.07

  • 鈴木克彦.顆粒球からみた防衛体力

    第56回日本体力医学会大会(シンポジウムⅢ あなたの免疫力はどこまでわかるか?−防衛体力の指標を求めて−) 

    Presentation date: 2001.09

  • Exercise-induced humoral modulation of phagocytes by cytokines and antioxidants.

    Suzuki K

    5th International Society of Exercise and Immunology Convention  (Baltimore)  International Society of Exercise and Immunology

    Presentation date: 2001.05

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Specific Research

  • 運動・ストレス・老化に関する新規バイオマーカーの開発と機能解析

    2019  

     View Summary

    本研究では、マウスの骨格筋を1Hz、2秒間隔で1時間電気刺激し、筋の反復収縮を確認しつつ、放出因子の特性について解析した。まずエクソソームをNanoSightで分析したところ、エクソソームの粒径は50-200 nm に94.9%が存在し、102 nmにピークが認められた。また、液体クロマトグラフィーを用いてNonoLC-MS/MS 分析を行ったところ、プロテオミクスでは248種類もの物質がリストされた。NCBI databaseにより機能解析を進めたところ、他臓器に影響を及ぼす可能性がある47の生理活性物質が認められたが、興味深いことにマイオカインの代表とされてきたインターロイキン6(IL-6)は検出されなかった。一方、血液の一般生化学検査では腎機能の改善が認められたため、そのメカニズムについてリストアップされた物質との関連を解析する予定である。

  • 加齢性筋機能低下における翻訳速度変化とその原因因子の同定

    2018  

     View Summary

    &nbsp;本研究では、網羅的な翻訳動態解析が可能なリボソームプロファイリングと網羅的転写動態解析を活用して、マウスの運動モデルにおいて運動トレーニングに対する骨格筋適応機序を網羅的見知から解明することを目的とした。マウス運動モデルに加え、加齢マウスモデルの骨格筋サンプルを用いて、転写・翻訳動態における網羅的解析を行った。さらに、骨格筋におけるシステムレベルでのネットワーク変化を捉えるために、共発現遺伝子ネットワーク解析を行ったところ、翻訳動態特異的なネットワークが存在する可能性が示唆された。このネットワークを詳細に検討したところ、これまでリボソームや翻訳とは全く関係性が認められていない遺伝子も当該ネットワークに存在していた。

  • 一過性持久性運動における翻訳動態変化の網羅的解析

    2016   佐古博皓

     View Summary

    Ribosome profilingは、翻訳阻害剤等で翻訳中のリボソームをmRNA上に固定し、リボソームに包含されていないmRNA領域を酵素分解し、リボソームに包含され翻訳されていた領域を抽出・精製し次世代シーケンサーで解析することで、それぞれのmRNAが実際にどの程度翻訳されていたのかを網羅的に解析できる。本研究では、マウスの持久性運動によって骨格筋の個々の遺伝子の翻訳動態がどのように変化するのかを検討したが、運動の有無よりも転写動態と翻訳動態プロファイルとの間の差が大きいことが認められた。また、運動により転写・翻訳動態が有意に変動する遺伝子を解析したところ、転写レベルでは有意差が認められず翻訳動態のみが有意に増加した遺伝子が見つかった。

  • 運動・ストレス・老化に関する新規バイオマーカーの開発と予防医学・臨床医学への応用

    2015   矢田 光一, 佐古 博皓, 荻野目 夏望

     View Summary

    運動により骨格筋において血管新生やミトコンドリア新生が生じるが,この現象には老化遺伝子として注目されているSirt-1が関与している.近年,食品に含まれる機能性成分としてポリフェノールが注目されているが,ある種のポリフェノールも同様にSirt-1の発現亢進を介してミトコンドリア新生を亢進すると報告されている.そこで,運動時のポリフェノール摂取が骨格筋における血管新生およびミトコンドリア新生に及ぼす影響について検討した.C57BL/6雄性マウスをポリフェノール摂取の有無および運動の有無により4群に分けた.運動開始の1時間前にポリフェノールを200 mg/kg weight経口投与した.運動群のマウスには速度18m/min,5%の傾斜から始め,疲労困憊に至るまで30分おきに3 m/min速度を上げるトレッドミル走を負荷した.運動直後に解剖を行い,腓腹筋を採取した.運動関連遺伝子としてperoxisomeproliferatoractivated receptor γ coactivator-1(PGC-1)α,シトクロムc(Cytoc),COXⅣ,クエン酸合成酵素(CS),vascularendothelial growth factor(VEGF),Sirtuin1(Sirt-1)のmRNAをリアルタイムPCRで測定した.疲労困憊に至るまでの走行時間には,ポリフェノール投与の有無による差は認められなかった.また,運動によって骨格筋におけるPGC-1α,VEGF,Sirt-1の発現が有意に上昇したが,CS,Cytoc,COXⅣの発現は亢進しなかった.一方,ポリフェノール投与はいずれの遺伝子発現にも影響を及ぼさなかった.

  • 運動による抗酸化酵素の産生と動脈硬化症の抑制

    2013  

     View Summary

     動脈硬化症は、血管壁へ浸潤した免疫細胞の一種であるマクロファージがコレステロールを取り込み泡沫化することで発症する。これにともなう酸化ストレスの増大は動脈硬化症を促進することから、酸化ストレスを軽減することで動脈硬化症の発症を予防することが期待できる。運動は動脈硬化症の予防に効果的であることが運動介入研究や疫学研究により報告されているが、その分子メカニズムは明らかにされていない。本研究では、運動による動脈硬化症の抑制とその分子メカニズムについて、運動トレーニングによる抗酸化酵素の増加が酸化ストレスを軽減し動脈硬化症の発症を予防すると仮説を立て検証することを目的とした。トレーニングには自由走行ケージによる自発的な運動トレーニングを用いマウスのストレスを軽減した運動のみの効果を得られるよう工夫した。解剖はトレーニング期間最終日の一過性の運動の効果を取り除くため、運動トレーニング期間終了の24時間後に行った。血液は麻酔下で心臓より採血し、頸椎脱臼後、PBSにて灌流後に大動脈と骨格筋を採取した。動脈硬化巣の評価は採取した動脈から凍結検体を作成後、薄切しオイルレッドO染色にて評価した。骨格筋の抗酸化酵素の測定はウェスタンブロット法を用いた。その結果、運動トレーニング群はコントロール群に比べ摂餌量が多いにもかかわらず体重の増加は有意に抑制された。運動トレーニング群の骨格筋はトレーニング効果の指標である遅筋化が観察された。骨格筋抗酸化酵素は、運動トレーニング群はコントロール群に比べ分泌型の抗酸化酵素である細胞外抗酸化酵素の他、ミトコンドリアや細胞質の抗酸化酵素の発現を有意に増加した。分泌型の抗酸化酵素は血液中にも分泌されるため、血管の酸化ストレスを軽減し動脈硬化を抑制することが期待できる。現在動脈硬化症の組織学的評価と酸化ストレスの検討を進めており、運動トレーニングによる動脈硬化症の抑制が証明できれば、運動トレーニングによる動脈硬化症予防は、運動による抗酸化酵素の増加が一因であることを証明できる意義のある研究に発展すると考えている。

  • 新規好中球機能検査法の健康診断・臨床検査への応用

    2010  

     View Summary

     本研究では好中球機能検査法のなかでも、温度官能性ハイドロゲルを用いた血中好中球の接着・遊走能と活性酸素産生能の測定、および酵素免疫測定法による血漿のミエロペルオキシダーゼ(myeloperoxidase: MPO)とカルプロテクチン(calprotectin)の濃度測定を評価系として設定し、各種の酸化ストレスや炎症の条件化で検討を行った。好中球接着・遊走能については、従来の顕微鏡下での血球計算盤を用いた計測に加え、蛍光をによる核染色を用いた定量法も採用し、測定系の一部自動化にも取り組んだ。 好中球数の臨床検査としての応用については、炎症性腸疾患患者の白血球除去療法の治療経過のモニターとして検討したが、有意な影響は認められなかった。また高齢者では好中球機能が亢進し慢性炎症の状態となるが、身体活動量の高い者と低い者との間に有意差は認められなかった。一方、運動負荷の前後の検討では、伸張性筋活動の後にやや好中球機能が上昇する傾向がみられたが、数日後の遅発性筋肉痛の発症時には変化が認められなかった。一方、持久性運動負荷の後には血漿のミエロペルオキシダーゼとカルプロテクチン濃度の上昇が若年者でも中高年者でも認められた。よって、好中球機能は持久性運動によって亢進することが確かめられた。がん患者では健常者より好中球活性酸素産生能の亢進がみられ、抗酸化物質の使用により酸化ストレスの軽減効果が示唆された。そこで、各種植物抽出成分のin vitroでの機能性評価に応用したところ、ビタミンC、カテキン、ポリフェノールなど抗酸化作用が報告されている物質群では濃度依存性の有意な抑制効果が観察されたが、とくにそのような効果が報告されていない物質群では影響は認められず、このハイドロゲルを用いた好中球機能測定系が抗酸化作用、抗炎症作用のスクリーニング系として有用である可能性が示された。 そこで今後は、運動負荷による炎症反応や酸化ストレス、がん患者や高齢者の軽度の慢性炎症(chronic low-grade inflammation)への抗酸化物質の介入研究による評価に応用していくことになった。

  • 熱可逆ハイドロゲルを用いた新規好中球機能検査法の健康診断・臨床検査への応用

    2008   椎葉 大輔

     View Summary

     まずハイドロゲルを用いたシングルチューブによる標準型測定系について、従来は細胞数計測の部分を血球計算盤を用いて顕微鏡下に目視で行ってきたが、手作業で手間がかかる上に主観も入るため、客観的に短時間で定量的な評価を行うために自動分析装置を導入し、測定系の充実をはかり、細胞数と蛍光強度がほぼ比例関係にあることを確認した。 次に、好中球機能がストレスの評価指標となりうるかどうかを検討した。持久性運動負荷や手術侵襲では生体負担を反映して好中球活性酸素産生能が上昇し、他の酸化ストレスの生体指標よりも鋭敏に生体反応を捉えられることが示唆された。しかし、筋損傷を起こす伸張性運動負荷では、遅発性筋肉痛をはじめとする炎症症状やクレアチンキナーゼ等の筋損傷マーカーの上昇は顕在化するにもかかわらず、好中球活性酸素産生能の上昇は認められず、エネルギー枯渇やストレスホルモンの分泌が生じるストレス状態においてのみ測定値の上昇が生じる可能性が考えられた。 臨床検査への応用としては、重症肺炎や原因不明で難病指定となっている炎症性腸疾患(潰瘍性大腸炎、クローン病)の患者での治療前後の検討を30症例ほど検討し、診断や治療モニタリングの指標としてよく炎症状態を反映する可能性が示された。さらに各種抗炎症薬や抗酸化物質を用いたin vitroの作用解析を行い、この測定系が各種薬剤や健康食品成分の評価に応用できる研究成果を示し、日本補完代替医療学会にて発表した。

  • 新規好中球機能解析法によるストレス・免疫能の評価と健康診断・臨床検査への応用

    2007   奥津光晴

     View Summary

     メビオール株式会社との共同研究にて開発した白血球の遊走能と活性酸素産生能の測定系について、まず基礎的検討を進めた。好中球の遊走因子であるインターロイキン8については、200pg/ml以上の高濃度では遊走細胞数の増加を認めたものの、高価な試薬であるため臨床検査に用いるには難しいと判断された。一方、単球を遊走させるためにmonocyte chemotactic protein 1(MCP-1)を用いたが、基材との関係で遊走活性や活性酸素生成を検出することはできなかった。また活性酸素産生能を呈色反応として検出するためにnitroblue tetrazolium(NBT)を用いたが、化学発光とくらべ定量性、再現性に問題があり、NBTを臨床検査に用いるのは困難と考えられた。 次に、従来法を用いて各種物質、薬剤の好中球機能に及ぼす影響を検討した。ビタミンCは活性酸素生成を濃度依存的に抑制したが、遊走細胞数には影響を及ぼさず、本検査法は抗酸化物質の活性評価や酸化ストレスの評価に有用と考えられた。次にストレスホルモンの代表としてエプネフリンの作用を検討したが、濃度・作用時間の影響は認められなかった。一方、ハイドロコルチゾンは濃度依存的に遊走細胞数を減少させ、抗炎症作用・免疫抑制作用の評価に有用と考えられた。このように生理活性物質や薬物の作用解析・薬剤感受性の評価に好中球機能検査法が応用できる可能性を示した。 健康診断・臨床検査への応用に関しては、国立国際医療センターで臨床検査としての倫理申請が認められたので、今後臨床検体での検討を進め、妥当性の検証を進めることとなった。

  • 白血球活性酸素生成系の新しい解析法の開発と運動負荷研究への応用

    2005   今泉 和彦, 一之瀬 貴, 奥津 光晴

     View Summary

     白血球活性酸素産生能の測定系として、全血を温度官能性ゲルと接触させると白血球が浸潤することをメビオール社との共同研究にて検討し、その素材を利用してさらに遊走した白血球が産生する活性酸素をluminol依存性化学発光法やdihydrorhodamine 123, hydroethidine(蛍光)にて測定できるよう条件設定を進めた。 この測定系を運動負荷研究に応用したところ、白血球のゲルへの浸潤(遊走能)が高まる傾向を示した。ストレスホルモンやケモカインの変動とケモカイン受容体CCR2の発現との関連を調べたところ、CCR2はエピネフリン、ノルエピネフリンの影響を受けなかったが、コルチゾールの濃度に依存して増強した。さらにCCR2の発現の増強により、白血球の移動能力も増加した。この結果はストレスによって分泌されるストレスホルモンがCCR2の発現を増強させた可能性を示している。 

  • スポーツ医科学領域における活性酸素・サイトカイン研究の進展と統合

    2004   PEAKE, Jonathan

     View Summary

     サイトカインは、免疫系や炎症の制御分子として注目されている生理活性物質である。著者らは、マラソンのような激しい持久性運動によってサイトカインの血中濃度が変動することを明らかにしてきた。しかし、従来、運動負荷に伴う血中サイトカイン濃度の変動は、運動の強度に依存するものか、あるいは運動による骨格筋の損傷に伴うものであるのか明らかではなかった。そこで本研究では、最大酸素摂取量の60%強度と85%強度でそれぞれ60分間の水平トレッドミル走、最大酸素摂取量の60%強度で-10%の傾斜のあるダウンヒルのトレッドミル走(筋損傷が顕在化しやすい典型的運動負荷)の3条件を設定し、同一の被験者9名に3種類の運動を負荷して、運動条件間の比較検討を行った。運動強度の指標としては、コルチゾール、カテコールアミンなどのストレスホルモンの血中濃度を測定し、筋損傷の指標としては血中ミオグロビン濃度、クレアチンキナーゼ活性を測定したが、3条件はそれぞれの指標に特徴的に顕著な影響を及ぼし、実験条件の設定は良好であった。本研究では、9種類のサイトカインの測定を行ったが、IL-6、IL-1ra、IL-10、IL-12p40、MCP-1、HSP70は運動強度依存的に有意な上昇を示し、これに比し、筋損傷とは関連は認められなかった。これらのサイトカインは、細胞性免疫を抑制する作用があり、炎症の全身性波及を抑制する適応機構として働く反面、感染に対する抵抗能力を低下させる可能性が考えられる。またこれらのサイトカインの変動は、コルチゾール、カテコールアミンなどのストレスホルモンとの間に強い相関が認められ、神経内分泌因子と免疫系の連動が密接であることを証明できた。さらにこのような全身的なストレス要因を排除して筋損傷の影響を検討するために、局所的な肘進展モデルを用いた遅発性筋炎に伴う血中サイトカイン濃度の変動も検討したが、筋損傷マーカーの劇的な変動にも関わらずサイトカインの変動は軽微であり、血中サイトカイン濃度の変動は運動強度(ストレス)依存性であることが確認できた。これらの成果は、米国生理学会にて報告し、また5月のアジアスポーツ医学会で報告する予定であり、既に掲載された論文と現在米国スポーツ医学会誌に投稿中の論文が成果として出つつある。

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Overseas Activities

  • 運動・ストレス・老化に関する新規バイオマーカーの開発

    2013.09
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    2014.09

    オーストラリア   クイーンズランド工科大学

 

Syllabus

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Committee Memberships

  • 2019.09
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    Now

    Frontiers in Sports and Active Living  Associate Editor

  • 2019.09
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    Now

    Frontiers in Physiology (IF 3.394)  Associate Editor

  • 2014
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    Now

    早稲田大学 スポーツ医科学クリニック 運営委員など

  • 2013.09
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    Now

    国際運動免疫学会次期会長・学会誌編集委員

  • 2012.10
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    Now

    早稲田大学保健センター管理委員会委員

  • 2011
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    Now

    一般社団法人 エビデンスに基づく統合医療研究会 理事、評議員

  • 2010
    -
    Now

    早稲田大学 スポーツ科学部 教務委員会委員

  • 2009
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    Now

    早稲田大学 人を対象とする研究に関する倫理委員会 専門委員・委員

  • 2007
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    Now

    NPO法人 健康早稲田の杜 理事

  • 2006
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    Now

    日本体力医学会 評議員

  • 2006
    -
    Now

    日本補完代替医療学会 学会誌編集委員

  • 2002
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    Now

    日本運動免疫学研究会 運営委員 (2004年9月 第2回大会世話人)

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    -
    2021.03

    Vessel Plus  Guest Editor

  •  
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    2020.03

    Antioxidants  Guest Editor

  • 2018
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    2018.03

    Antioxidants  Guest Editor

  • 2014
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    栄養フロンティア誌  運動スポーツ栄養分野の編集委員

  • 2014
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    Frontiers in Nutrition.  Editorial Board of Sport and Exercise Nutrition

  • 2013
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    国際運動免疫学会  編集委員、次期会長

  • 2013
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    International Society of Exercise and Immunology  Editorial Board, President Elect

  •  
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    2012.09

    2010年〜2012年9月 早稲田大学 スポーツ科学部 運営委員会委員

  • 2011
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    一般社団法人 エビデンスに基づく統合医療研究会  理事、評議員

  • 2011
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    Association for Evidence-based Integrative Medicine  Board

  • 2006
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    日本補完代替医療学会  編集委員

  • 2006
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    日本体力医学会  評議員

  • 2006
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    The Japanese Society of Complementary and Alternative Medicine  Editor

  • 2006
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    The Japanese Society of Physical Fitness and Sports Medicine  Fellow

  • 2002
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    2005

    好中球研究会(RAND) 運営委員

  • 2002
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    日本運動免疫学研究会  運営委員

  • 2002
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    Japanese Society of Exercise and Immunology  Managing Board

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Social Activities

  • TBS 人体科学ミステリー アノ有名人のカラダを最新科学で大解剖SP

    TBS 人体科学ミステリー アノ有名人のカラダを最新科学で大解剖SP 

    2013.09
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    80歳でエベレスト登頂に成功した冒険家三浦雄一郎氏の運動負荷試験と血液検査を担当した。
    http://6mj.tv/episode_3FF28363C7EC1E35309D748AC0F9DDDA.html

  • NHKスペシャル取材班 『42.195kmの科学』 角川書店

    NHKスペシャル取材班 『42.195kmの科学』 角川書店 

    2013.02
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    マラソンによる臓器傷害とメカニズムについて取材を受けた内容が紹介された(68-71ページ)。
    http://www.kadokawa.co.jp/book/bk_detail.php?pcd=321210000042

  • 毎日新聞

    毎日新聞 

    2013.01
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     View Summary

    運動と免疫に関する記事作成で取材を受けた。
    http://mainichi.jp/feature/news/20130119ddm010100004000c.html

  • 日経ヘルスプルミエ(日経BP社)

    日経ヘルスプルミエ(日経BP社) 

    2012.12
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    運動と免疫、健康増進に関する記事作成で取材を受けた。
    http://www.shop-nv.jp/35.html

  • Tarzan(マガジンハウス)

    Tarzan(マガジンハウス) 

    2012.10
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    運動と免疫に関する記事作成で取材を受け、激運動による免疫機能低下について解説した。

  • 日経ヘルス(日経BP社)

    日経ヘルス(日経BP社) 

    2012.08
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    「意外に知られていない運動と免疫の関係 “適度”な運動で免疫力を高めよう」に関する記事作成で取材を受けた。
    http://wol.nikkeibp.co.jp/article/trend/20120727/130901/?P=2

  • 毎日新聞

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