Updated on 2022/05/18

写真a

 
NAGASHIMA, Kei
 
Affiliation
Faculty of Human Sciences, School of Human Sciences
Job title
Professor
Profile
Research focus is thermoregulation in humans and animals. Recent research project aims to clarify the mechanism involved in heat acclimation, and using the results develop a recipe to be heat acclimated.

Concurrent Post

  • Faculty of Commerce   School of Commerce

  • Faculty of Human Sciences   Graduate School of Human Sciences

  • Affiliated organization   Global Education Center

Education

  •  
    -
    1995

    Kyoto Prefectural University of Medicine   Graduate School, Division of Medicine  

  •  
    -
    1986

    Kyoto Prefectural University of Medicine   Faculty of Medicine  

Degree

  • MBBS

  • PhD

Research Experience

  • 2004
    -
     

    Associate Professor, Professor, Faculty of Human Sciences, Waseda University

  • 1999
    -
    2004

    Assistant Professor, School of Medicin, Osaka University

  • 1998
    -
    1999

    Fellow, Royal North Shore Hospital

  • 1995
    -
    1998

    Postdoctoral associate Yale Univ. School of Med. and The John B. Pierce Laboratory

Professional Memberships

  •  
     
     

    日本外科学会

  •  
     
     

    時間生物学会

  •  
     
     

    日本体力医学会

  •  
     
     

    American Physiological Society

  •  
     
     

    Noth American Federation of Nueroscience

  •  
     
     

    日本神経科学会

  •  
     
     

    日本生理学会

  •  
     
     

    日本生気象学会

▼display all

 

Research Areas

  • Physiology

Research Interests

  • body temperature, body fluid, temperature sensation, circadian rhythm, sex hormones, exercise physiology

Papers

  • Regular exercise stimulates endothelium autophagy via IL-1 signaling in ApoE deficient mice.

    Mitsuharu Okutsu, Mami Yamada, Ken Tokizawa, Shuri Marui, Katsuhiko Suzuki, Vitor A Lira, Kei Nagashima

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology   35 ( 7 ) e21698  2021.07  [International journal]

     View Summary

    Regular exercise maintains arterial endothelial cell homeostasis and protects the arteries from vascular disease, such as peripheral artery disease and atherosclerosis. Autophagy, which is a cellular process that degrades misfolded or aggregate proteins and damaged organelles, plays an important role in maintaining organ and cellular homeostasis. However, it is unknown whether regular exercise stimulates autophagy in aorta endothelial cells of mice prone to atherosclerosis independently of their circulating lipid profile. Here, we observed that 16 weeks of voluntary exercise reduced high-fat diet-induced atherosclerotic plaque formation in the aortic root of ApoE deficient mice, and that this protection occurred without changes in circulating triglycerides, total cholesterol, and lipoproteins. Immunofluorescence analysis indicated that voluntary exercise increased levels of the autophagy protein LC3 in aortic endothelial cells. Interestingly, human umbilical vein endothelial cells (HUVECs) exposed to serum from voluntarily exercised mice displayed significantly increased LC3-I and LC3-II protein levels. Analysis of circulating cytokines demonstrated that voluntary exercise caused changes directly relevant to IL-1 signaling (ie, decreased interleukin-1 receptor antagonist [IL-1ra] while also increasing IL-1α). HUVECs exposed to IL-1α and IL-1β recombinant protein significantly increased LC3 mRNA expression, LC3-I and LC3-II protein levels, and autophagy flux. Collectively, these results suggest that regular exercise protects arteries from ApoE deficient mice against atherosclerosis at least in part by stimulating endothelial cell autophagy via enhanced IL-1 signaling.

    DOI PubMed

  • Influence of exogenous and endogenous estrogen on thermoregulatory responses to mild heat and the interaction with light and dark phases.

    Shuri Marui, Yuta Masuda, Issei Kato, Kei Nagashima

    The journal of physiological sciences : JPS   70 ( 1 ) 56 - 56  2020.11  [Domestic journal]

     View Summary

    The present study aimed to determine the influence of estradiol (E2) and the interaction with circadian phases on thermoregulatory responses to mild heat in female rats. Heat loss and production during 3-h exposure to the environment at an ambient temperature of 28-34 °C were assessed by measuring abdominal temperature (Tabd), tail skin temperature, and oxygen consumption in ovariectomized rats with and without E2 replacement (OVX + E2 and OVX, respectively) and in control rats in the proestrus (P) and diestrus (D) phases. In the light phase, Tabd remained unchanged in all groups. Tabd increased in the dark phase, but was lower in the OVX + E2 and P groups than in the OVX and D groups. Oxygen consumption decreased at 34 °C, but to a lesser extent in the OVX + E2 group than in the OVX group. These results suggest that E2 activates thermoregulation in mild heat in the dark phase.

    DOI PubMed

  • 体液を一定に保つ仕組み

    永島 計

    体育の科学   70 ( 2 ) 95 - 99  2020.02  [Refereed]

    CiNii

  • "Factors affecting an increase in core body temperature and heat tolerance during hot water immersion"

    Yuta Masuda, Issei Kato, Kei Nagashima

       2020

  • Muscle-derived SDF-1α/CXCL12 modulates endothelial cell proliferation but not exercise training-induced angiogenesis.

    Mami Yamada, Chihiro Hokazono, Ken Tokizawa, Shuri Marui, Masahiro Iwata, Vitor A Lira, Katsuhiko Suzuki, Shinji Miura, Kei Nagashima, Mitsuharu Okutsu

    American journal of physiology. Regulatory, integrative and comparative physiology   317 ( 6 ) R770-R779  2019.12  [Refereed]  [International journal]

     View Summary

    Chemokines are critical mediators of angiogenesis in several physiological and pathological conditions; however, a potential role for muscle-derived chemokines in exercise-stimulated angiogenesis in skeletal muscle remains poorly understood. Here, we postulated that the chemokine stromal cell-derived factor-1 (SDF-1α/C-X-C motif chemokine ligand 12: CXCL12), shown to promote neovascularization in several organs, contributes to angiogenesis in skeletal muscle. We found that CXCL12 is abundantly expressed in capillary-rich oxidative soleus and exercise-trained plantaris muscles. CXCL12 mRNA and protein were also abundantly expressed in muscle-specific peroxisome proliferator-activated receptor γ coactivator 1α transgenic mice, which have a high proportion of oxidative muscle fibers and capillaries when compared with wild-type littermates. We then generated CXCL12 muscle-specific knockout mice but observed normal baseline capillary density and normal angiogenesis in these mice when they were exercise trained. To get further insight into a potential CXCL12 role in a myofiber-endothelial cell crosstalk, we first mechanically stretched C2C12 myotubes, a model known to induce stretch-related chemokine release, and observed increased CXCL12 mRNA and protein. Human umbilical vein endothelial cells (HUVECs) exposed to conditioned medium from cyclically stretched C2C12 myotubes displayed increased proliferation, which was dependent on CXCL12-mediated signaling through the CXCR4 receptor. However, HUVEC migration and tube formation were unaltered under these conditions. Collectively, our findings indicate that increased muscle contractile activity enhances CXCL12 production and release from muscle, potentially contributing to endothelial cell proliferation. However, redundant signals from other angiogenic factors are likely sufficient to sustain normal endothelial cell migration and tube formation activity, thereby preserving baseline capillary density and exercise training-mediated angiogenesis in muscles lacking CXCL12.

    DOI PubMed

  • 体温調節の脳機構と加齢による変容ー特に温度感覚と行動性体温調節の観点からー

    永島 計

    基礎老化研究   43 ( 3 ) 17 - 22  2019.09  [Refereed]

  • Assessment of brain mechanisms involved in the processes of thermal sensation, pleasantness/unpleasantness, and evaluation.

    Yuka Aizawa, Tokiko Harada, Hiroki Nakata, Mizuki Tsunakawa, Norihiro Sadato, Kei Nagashima

    IBRO reports   6   54 - 63  2019.06  [Refereed]  [International journal]

     View Summary

    The conscious perception of thermal stimuli is divided into two categories: thermal sensation (i.e., discriminative component) and pleasantness/unpleasantness (i.e., hedonic component). There have been very few studies which clearly dissociated the two components. The aim of the present study was 1) to identify brain regions involved in perception of thermal stimuli per se, dissociating those related to the two components, and additionally 2) to examine brain regions of the explicit evaluation processes for the two components. Sixteen participants received local thermal stimuli of either 41.5 °C or 18.0 °C during whole-body thermal stimuli of 47.0 °C, 32.0 °C, or 17.0 °C. The local stimuli were delivered to the right forearm with the Peltier device. The whole-body stimuli delivered through a water-perfusion suit was aimed to modulate thermal pleasantness/unpleasantness to the local stimulus. The local stimulation at the same temperature was conducted five times with 30-s intervals. Brain activation was assessed by functional magnetic resonance imaging (fMRI), and the participants were asked to report their ratings of thermal sensation and pleasantness/unpleasantness following the cessation of each local stimulus. Local thermal stimulation activated specific brain regions such as the anterior cingulate cortex, insula, and inferior parietal lobe, irrespective of the temperature of local and whole-body stimuli; however, no specific activation for hot or cold sensation was observed. Different brain regions were associated with pleasantness and unpleasantness; the caudate nucleus and frontal regions for pleasantness, and the medial frontal and anterior cingulate cortex for unpleasantness. In addition, the explicit evaluation process for the discriminative and hedonic components immediately following the cessation of local stimulus involved different brain regions; the medial prefrontal cortex extending to the anterior cingulate cortex, insula, middle frontal cortex, and parietal lobes during the explicit evaluation of thermal sensation, and the medial prefrontal cortex, posterior cingulate cortex, and inferior parietal lobes during that of pleasantness/unpleasantness.

    DOI PubMed

  • Thermal and cardiovascular responses and thermal sensation during hot-water bathing and the influence of room temperature.

    Yuta Masuda, Shuri Marui, Issei Kato, Mayuka Fujiki, Mariko Nakada, Kei Nagashima

    Journal of thermal biology   82   83 - 89  2019.05  [Refereed]  [International journal]

     View Summary

    The aim of the present study was to clarify physical risks during hot-water bathing by measuring thermal and cardiovascular responses and thermal sensation. Young men and women (n = 7 and 5, respectively) participated in the present study, which consisted of two trials mimicking bathing behavior at room temperature of 25 °C and 15 °C. Participants bathed in 41 °C water for 20 min to the subclavian level. Before bathing, participants rested fully clothed for 15 min and then rested for 15 min without clothes. After bathing, they rested without clothes for 15 min and afterwards rested fully clothed for another 15 min. Tympanic temperature (Tty), heart rates (HR), mean skin temperature (Tsk), mean arterial pressure (MAP), and laser-Doppler flow at the chest and forehead (LDFhead and LDFchest) were evaluated. Thermal perception was assessed with a visual analogue scale. Mean Tsk in the 15 °C trial decreased during the period without clothing while MAP increased. The value remained unchanged in the 25 °C trial. During bathing, Tty, mean Tsk, HR, LDFhead, and LDFchest increased in both trials, and MAP decreased to similar levels. Relative change in LDFchest was greater in the 15 °C trial than in the 25 °C trial. Participants felt cold when they were without clothes at 15 °C; however, the thermal perception during bathing was similar between the two trials. Greater changes in cardiovascular and thermal responses were observed during the bathing behavior. In addition, bathing in cold room augmented the changes, which may induce some physical risks during bathing.

    DOI PubMed

  • 体温調節の発達 体温および体液研究から

    永島 計

    月刊トレーニング・ジャーナル   41 ( 5 ) 12 - 15  2019.05  [Refereed]

    CiNii

  • 脱水にならないこと、そして脱水を見逃さないこと

    永島 計

    コーチング・クリニック   33 ( 9 ) 28 - 31  2019.04  [Refereed]

    CiNii

  • 高体温による身体のダメージ―熱中症― 温度変化への身体適応5

    永島 計

    体育の科学   69 ( 3 ) 215 - 219  2019.03  [Refereed]

    CiNii

  • 女性と体温 温度変化への身体適応4

    永島 計

    体育の科学   69 ( 2 ) 133 - 137  2019.02  [Refereed]

    CiNii

  • 子どもと高齢者の体温調節の特徴 温度変化への身体適応3

    永島 計

    体育の科学   69 ( 1 ) 53 - 56  2019.01  [Refereed]

    CiNii

  • 運動時の体温適応メカニズム 温度変化への身体適応2

    永島 計

    体育の科学   68 ( 12 ) 909 - 913  2018.12  [Refereed]

    CiNii

  • 暑熱環境や寒冷環境にどう適応するか 温度変化への身体適応1

    永島 計

    体育の科学   68 ( 11 ) 833 - 837  2018.11

    CiNii

  • Fasting or systemic des-acyl ghrelin administration to rats facilitates thermoregulatory behavior in a cold environment.

    Yuki Uchida, Kei Nagashima, Kazunari Yuri

    Brain research   1696   10 - 21  2018.10  [Refereed]  [International journal]

     View Summary

    Fasted rats place their tails underneath their body trunks in the cold (tail-hiding behavior), which is a thermoregulatory behavior. The aim of the present study was to investigate the effect of fasting and des-acyl ghrelin, a hormone related to fasting, on tail-hiding behavior and neural activity in the cold. Wistar rats were divided into 'fed', '42-h fasting' and des-acyl ghrelin groups. The rats received an intraperitoneal saline or 30-μg des-acyl ghrelin injection, and were then exposed to 27 °C or 15 °C for 2-h with continuous body temperature (Tb), tail skin temperature (Ttail), and tail-hiding behavior measurements. cFos immunoreactive (cFos-IR) cells in the insula, secondary somatosensory cortex, medial preoptic nucleus, parastrial nucleus, amygdala, and lateral parabrachial nucleus were counted in four segments: seg1, 2, 3, and 4 (bregma -0.36, -1.44, -2.64, and -9.00 mm), respectively. At 15 °C, Tb and Ttail were lower in the 42-h fasting group than in the fed and des-acyl ghrelin groups, and the duration of tail-hiding behavior was longer in the 42-h fasting and des-acyl ghrelin groups than in the fed group. The onset of tail-hiding behavior more advanced in the des-acyl ghrelin group than in the fed group at 15 °C. Only at the insula in seg3 at 15 °C, the number of cFos-IR cells was greater in the 42-h fasting group than in the fed group. Both the 42-h fasting and des-acyl ghrelin groups might modulate the tail-hiding behavior of rats in a cold, and a part of the insula might be involved this response during fasting.

    DOI PubMed

  • Regional differences of cFos immunoreactive cells in the preoptic areas in hypothalamus associated with heat and cold responses in mice.

    Yuki Uchida, Keisuke Onishi, Ken Tokizawa, Kei Nagashima

    Neuroscience letters   665   130 - 134  2018.02  [Refereed]  [International journal]

     View Summary

    cFos expression in the preoptic area (PO), which is thermoregulatory center increased by both heat and cold exposures; however, the regional difference is unknown yet. We aimed to determine if cFos expression in the PO was regionally different between heat and cold exposures. Mice were exposed to 27, 10, or 38°C for 90min, and body temperature (Tb) was measured. cFos-immunoreactive (cFos-IR) cells in the PO were counted by separating the PO into the ventral and dorsal parts in the rostral (bregma 0.38mm), central (-0.10mm), and caudal (-0.46mm) planes. Tb at 10°C remained unchanged; however, it increased at 38°C. Counts of cFos-IR cells in all areas were greater at 38°C than at 27°C. In the dorsal and ventral parts of the central and the dorsal part of caudal PO, counts of cFos-IR cells were greater at 10°C than at 27°C. In conclusion, the areas of increased cFos expression in the PO in the heat were different that in the cold in mice.

    DOI PubMed

  • Thermal comfort.

    Kei Nagashima, Ken Tokizawa, Shuri Marui

    Handbook of clinical neurology   156   249 - 260  2018  [Refereed]  [International journal]

     View Summary

    The processes of thermoregulation are roughly divided into two categories: autonomic and behavioral. Behavioral thermoregulation alone does not have the capacity to regulate core temperature, as autonomic thermoregulation. However, behavioral thermoregulation is often utilized to maintain core temperature in a normal environment and is critical for surviving extreme environments. Thermal comfort, i.e., the hedonic component of thermal perception, is believed to be important for initiating and/or activating behavioral thermoregulation. However, the mechanisms involved are not fully understood. Thermal comfort is usually obtained when thermal stimuli to the skin restore core temperature to a regulated level. Conversely, thermal discomfort is produced when thermal stimuli result in deviations of core temperature away from a regulated level. Regional differences in the thermal sensitivity of the skin, hypohydration, and adaptation of the skin may affect thermal perception. Thermal comfort and discomfort seem to be determined by brain mechanisms, not by peripheral mechanisms such as thermal sensing by the skin. The insular and cingulate cortices may play a role in assessing thermal comfort and discomfort. In addition, brain sites involved in decision making may trigger behavioral responses to environmental changes.

    DOI PubMed

  • Body temperature and cold sensation during and following exercise under temperate room conditions in cold-sensitive young trained females.

    Naoto Fujii, Erii Aoki-Murakami, Bun Tsuji, Glen P Kenny, Kei Nagashima, Narihiko Kondo, Takeshi Nishiyasu

    Physiological reports   5 ( 20 )  2017.11  [Refereed]  [International journal]

     View Summary

    We evaluated cold sensation at rest and in response to exercise-induced changes in core and skin temperatures in cold-sensitive exercise trained females. Fifty-eight trained young females were screened by a questionnaire, selecting cold-sensitive (Cold-sensitive, n = 7) and non-cold-sensitive (Control, n = 7) individuals. Participants rested in a room at 29.5°C for ~100 min after which ambient temperature was reduced to 23.5°C where they remained resting for 60 min. Participants then performed 30-min of moderate intensity cycling (50% peak oxygen uptake) followed by a 60-min recovery. Core and mean skin temperatures and cold sensation over the whole-body and extremities (fingers and toes) were assessed throughout. Resting core temperature was lower in the Cold-sensitive relative to Control group (36.4 ± 0.3 vs. 36.7 ± 0.2°C). Core temperature increased to similar levels at end-exercise (~37.2°C) and gradually returned to near preexercise rest levels at the end of recovery (>36.6°C). Whole-body cold sensation was greater in the Cold-sensitive relative to Control group during resting at a room temperature of 23.5°C only without a difference in mean skin temperature between groups. In contrast, cold sensation of the extremities was greater in the Cold-sensitive group prior to, during and following exercise albeit this was not paralleled by differences in mean extremity skin temperature. We show that young trained females who are sensitive to cold exhibit augmented whole-body cold sensation during rest under temperate ambient conditions. However, this response is diminished during and following exercise. In contrast, cold sensation of extremities is augmented during resting that persists during and following exercise.

    DOI PubMed

  • Effect of Systemic Estradiol Administration on Circadian Body Temperature and Activity Rhythms in Female Rats

    Yuki Uchida, Shuri Marui, Ken Tokizawa, Kei Nagashima

    Anatomy & Physiology   7 ( 5 )  2017.09  [Refereed]  [Invited]

  • Systemic estradiol administration to ovariectomized rats facilitates thermoregulatory behavior in a cold environment.

    Yuki Uchida, Kei Nagashima, Kazunari Yuri

    Brain research   1670   125 - 134  2017.09  [Refereed]  [International journal]

     View Summary

    Rats place their tails underneath their bodies in the cold (tail-hiding behavior), which is a behavioral indicator of thermoregulation. The aim of the present study was to elucidate the effect of estradiol (E2) on tail-hiding behavior and neural activity assessed by immunohistochemistry. Ovariectomized rats were implanted with a silastic tube with or without E2 underneath the dorsal skin (E2(-) and E2(+) groups), and exposed to 27°C, 16°C, and 10°C for 2h with continuous body temperature (Tb), tail skin temperature (Ttail), and behavioral measurements. cFos immunoreactive (cFos-IR) cells in the insula, secondary somatosensory cortex, medial preoptic nucleus, parastrial nucleus, amygdala, and lateral parabrachial nucleus were counted. Tb and Ttail were not different between the E2(-) and E2(+) groups. At 16°C, the duration and the onset of tail-hiding behavior in the E2(+) group were greater than that in the E2(-) group. The number of cFos-IR cells in the insula of the E2(-) group was greater than that of the E2(+) group in rats kept at 16°C. E2 might modulate tail-hiding behavior of female rats at 16°C, and the insula may be involved in the response.

    DOI PubMed

  • Muscle contractile activity regulates SDF-1 alpha/CXCL12 expression in skeletal muscle

    Mami Yamada, Chihiro Hokazono, Ken Tokizawa, Shuri Marui, Masahiro Iwata, Katsuhiko Suzuki, Shinji Miura, Kei Nagashima, Mitsuharu Okutsu

    FASEB JOURNAL   31  2017.04  [Refereed]

  • Assessment of axillary temperature for the evaluation of normal body temperature of healthy young adults at rest in a thermoneutral environment.

    Shuri Marui, Ayaka Misawa, Yuki Tanaka, Kei Nagashima

    Journal of physiological anthropology   36 ( 1 ) 18 - 18  2017.02  [Refereed]  [International journal]

     View Summary

    BACKGROUND: The aims of this study were to (1) evaluate whether recently introduced methods of measuring axillary temperature are reliable, (2) examine if individuals know their baseline body temperature based on an actual measurement, and (3) assess the factors affecting axillary temperature and reevaluate the meaning of the axillary temperature. METHODS: Subjects were healthy young men and women (n = 76 and n = 65, respectively). Three measurements were obtained: (1) axillary temperature using a digital thermometer in a predictive mode requiring 10 s (T ax-10 s), (2) axillary temperature using a digital thermometer in a standard mode requiring 10 min (T ax-10 min), and (3) tympanic membrane temperature continuously measured by infrared thermometry (T ty). The subjects answered questions about eating and exercise habits, sleep and menstrual cycles, and thermoregulation and reported what they believed their regular body temperature to be (T reg). RESULTS: T reg, T ax-10 s, T ax-10 min, and T ty were 36.2 ± 0.4, 36.4 ± 0.5, 36.5 ± 0.4, and 36.8 ± 0.3 °C (mean ± SD), respectively. There were correlations between T ty and T ax-10 min, T ty and T ax-10 s, and T ax-10 min and T ax-10 s (r = .62, r = .46, and r = .59, respectively, P < .001), but not between T reg and T ax-10 s (r = .11, P = .20). A lower T ax-10 s was associated with smaller body mass indices and irregular menstrual cycles. CONCLUSIONS: Modern devices for measuring axillary temperature may have changed the range of body temperature that is recognized as normal. Core body temperature variations estimated by tympanic measurements were smaller than those estimated by axillary measurements. This variation of axillary temperature may be due to changes in the measurement methods introduced by modern devices and techniques. However, axillary temperature values correlated well with those of tympanic measurements, suggesting that the technique may reliably report an individual's state of health. It is important for individuals to know their baseline axillary temperature to evaluate subsequent temperature measurements as normal or abnormal. Moreover, axillary temperature variations may, in part, reflect fat mass and changes due to the menstrual cycle.

    DOI PubMed

  • Assessment of axillary temperature for the evaluation of normal body temperature of healthy young adults at rest in a thermoneutral environment

    Shuri Marui, Ayaka Misawa, Yuki Tanaka, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL ANTHROPOLOGY   36  2017.02  [Refereed]

     View Summary

    Background: The aims of this study were to (1) evaluate whether recently introduced methods of measuring axillary temperature are reliable, (2) examine if individuals know their baseline body temperature based on an actual measurement, and (3) assess the factors affecting axillary temperature and reevaluate the meaning of the axillary temperature.
    Methods: Subjects were healthy young men and women (n= 76 and n = 65, respectively). Three measurements were obtained: (1) axillary temperature using a digital thermometer in a predictive mode requiring 10 s (Tax-10 s), (2) axillary temperature using a digital thermometer in a standard mode requiring 10 min (Tax-10 min), and (3) tympanic membrane temperature continuously measured by infrared thermometry (T-ty). The subjects answered questions about eating and exercise habits, sleep and menstrual cycles, and thermoregulation and reported what they believed their regular body temperature to be (T-reg).
    Results: T-reg, Tax-10 s, Tax-10 min, and T-ty were 36.2 +/- 0.4, 36.4 +/- 0.5, 36.5 +/- 0.4, and 36.8 +/- 0.3 degrees C (mean +/- SD), respectively. There were correlations between T-ty and Tax-10 min, T-ty and Tax-10 s, and Tax-10 min and Tax-10 s (r = .62, r = .46, and r = .59, respectively, P &lt; .001), but not between T-reg and Tax-10 s (r = .11, P = .20). A lower Tax-10 s was associated with smaller body mass indices and irregular menstrual cycles.
    Conclusions: Modern devices for measuring axillary temperature may have changed the range of body temperature that is recognized as normal. Core body temperature variations estimated by tympanic measurements were smaller than those estimated by axillary measurements. This variation of axillary temperature may be due to changes in the measurement methods introduced by modern devices and techniques. However, axillary temperature values correlated well with those of tympanic measurements, suggesting that the technique may reliably report an individual's state of health. It is important for individuals to know their baseline axillary temperature to evaluate subsequent temperature measurements as normal or abnormal. Moreover, axillary temperature variations may, in part, reflect fat mass and changes due to the menstrual cycle.

    DOI

  • Influence of osmotic stress on thermal perception and thermoregulation in heat is different between sedentary and trained men.

    Ken Tokizawa, Mayumi Matsuda-Nakamura, Yuki Tanaka, Yuki Uchida, Cheng-Hsien Lin, Kei Nagashima

    Physiology & behavior   161   66 - 73  2016.07  [Refereed]  [International journal]

     View Summary

    Hyperosmolality in extracellular fluid in humans attenuates autonomic thermoregulation in heat, such as sweating and blood flow in the skin. However, exercise training minimizes the attenuation. The aim of the present study was to clarify the influence of hyperosmolality on thermal perception and to assess the training effect of exercise. Ten sedentary (SED) and 10 endurance-trained (TR) healthy young men were infused with 0.9% (normal saline [NS]) or 3% NaCl (hypertonic saline [HS]) for 120min on two separate days. After infusion for 20min, heat stimulus to the skin of the whole body was produced by a gradual increase in hot water-perfused suit temperature (33°C, 36°C, and 39°C), which was first used in the normothermic condition and then in the mild hyperthermic condition (0.5-0.6°C increase in esophageal temperature) and controlled by immersion of the lower legs in a water bath at 34.5°C and 42°C, respectively. Thermal sensation and comfort were rated at the time of each thermal condition. Plasma osmolality increased by ~10mosmL/kg·H2O in the HS trial. In the mild hyperthermic condition, increases in sweat rate and cutaneous vascular conductance were lower in the HS than in the NS trial in both the SED and TR groups (p<0.05). In the SED group, thermal sensation in the mild hyperthermic condition was lower in the HS than in the NS trial (p<0.05); there was no significant difference between the trials in the TR group. These results might indicate that hyperosmolality attenuates thermal sensation with heat and that exercise training eliminates the attenuation.

    DOI PubMed

  • Daily Changes of Body Temperature and Heart Rate are Modulated after Estradiol Depletion in Female Rats

    Shuri Marui, Yuki Uchida, Kei Nagashima

    Anatomy & Physiology   6 ( 1 ) 2 - 7  2016.01  [Refereed]

    DOI

  • Estrogenic modulation of female thermoregulatory behavior in a cold environment

    Uchida Yuki, Nagashima Kei, Marui Shuri

    The Journal of Physical Fitness and Sports Medicine   5 ( 1 ) 77 - 80  2016  [Refereed]

     View Summary

    Thermoregulation is categorized as either autonomic (i.e., sweating, shivering) or behavioral (i.e., wearing clothes, usage of air conditioner) thermoregulation. Compared to autonomic thermoregulation, the neural pathway of behavioral thermoregulation in cold environments remains unclear. A decrease in ambient temperature is perceived through thermoreceptors for detecting cold, including transient receptor potential (TRP) channels, such as TRPM8 and TRPA1, which are expressed in the sensory nerve endings of the skin. From these receptors, nerves connect to the dorsal root ganglion and dorsal horn in the spinal cord, and arrive at the lateral parabrachial nucleus in the pons, which is the same neural pathway that is used for autonomic thermoregulation. Following this, an unknown neural pathway induces thermoregulatory behavior, such as cold-escape behavior. Both young and climacteric women complain of an unpleasant thermal comfort, which is attributed to &quot;hie-sho&quot; (chill or poor blood circulation). The altered thermal sensation and comfort by an absence or a fluctuation of estrogen (E2) may modulate behavioral thermoregulation in females. This effect is unknown in women. However, in

    DOI CiNii

  • Cold exposure and/or fasting modulate the relationship between sleep and body temperature rhythms in mice.

    Nobuo Sato, Shuri Marui, Makoto Ozaki, Kei Nagashima

    Physiology & behavior   149   69 - 75  2015.10  [Refereed]  [International journal]

     View Summary

    We assessed the relationship between core temperature (Tc) and sleep rhythms in mice, and examined the effects of ambient temperature and fasting. Tc, electroencephalograms (EEG), electromyograms (EMG), and spontaneous activity in male ICR mice (n=9) were measured by telemetry for 3 days under a 12:12h dark-light cycle. Mice were fed or fasted at an ambient temperature (Ta) of 27°C or 20°C for the final 30h of the experiment. The vigilance state was categorized into a wake state, rapid-eye movement (REM) sleep, and non-REM (NREM) sleep, and the total sleep time (TST) was assessed. Relationships between Tc and TST, NREM periods, and REM sleep were estimated using Pearson's correlation coefficient. During cold exposure, Tc decreased during the dark and light phases, and TST and the periods of NREM and REM sleep decreased during the dark phase. Throughout the fasting period, Tc also decreased during the dark and light phases. Furthermore, the decrease in Tc was augmented when fasting and cold were combined. TST and NREM sleep periods decreased in the light and dark phases, respectively, whereas REM sleep periods decreased in both phases. Negative linear correlations (r=-0.884 to -0.987) were observed between Tc and TST, NREM sleep periods, and REM sleep periods, except for Tc and REM sleep periods where fasting and cold conditions were combined. The correlations between sleep and Tc rhythms were well maintained during cold exposure and fasting. However, when cold and fasting were combined, REM sleep and Tc rhythms were desynchronized.

    DOI PubMed

  • Thermal information from the skin: the signal processing and the role in behavioral thermoregulation

    Kei Nagashima

    Temperature   2 ( 3 ) 334 - 335  2015.07  [Refereed]

    DOI PubMed

  • Thermoregulation and menstrual cycle

    Kei Nagashima

    Temperature   2 ( 3 ) 320 - 321  2015.07  [Refereed]

    DOI PubMed

  • Effect of menstrual cycle on thermal perception and autonomic thermoregulatory responses during mild cold exposure.

    Mayumi Matsuda-Nakamura, Saki Yasuhara, Kei Nagashima

    The journal of physiological sciences : JPS   65 ( 4 ) 339 - 47  2015.07  [Refereed]  [Domestic journal]

     View Summary

    We investigated the effects of menstrual cycle phase on thermal sensation, thermal pleasantness, and autonomic thermoregulatory responses during mild cold exposure. Eight healthy young women participated. Experiments were conducted in the follicular and luteal phases: 120 min exposure at 23.5 °C after 40-min at a baseline temperature of 29 °C. Body core temperature was higher (P = 0.01) in the luteal phase than in the follicular phase. Thermal sensation of the whole body (P = 0.59), hands (P = 0.46), and toes (P = 0.94), and thermal pleasantness of the whole body (P = 0.79) were no different between phases. In both phases, mean skin temperature decreased (P = 0.00) in the same manner without any change in metabolic rate (P = 0.90). These results suggest the change of body core temperature in the menstrual cycle phases has no effect on thermal perception of cold or on autonomic cold-defense response.

    DOI PubMed

  • Estimation of the core temperature control during ambient temperature changes and the influence of circadian rhythm and metabolic conditions in mice.

    Ken Tokizawa, Tamae Yoda, Yuki Uchida, Kazuyuki Kanosue, Kei Nagashima

    Journal of thermal biology   51   47 - 54  2015.07  [Refereed]  [International journal]

     View Summary

    It has been speculated that the control of core temperature is modulated by physiological demands. We could not prove the modulation because we did not have a good method to evaluate the control. In the present study, the control of core temperature in mice was assessed by exposing them to various ambient temperatures (Ta), and the influence of circadian rhythm and feeding condition was evaluated. Male ICR mice (n=20) were placed in a box where Ta was increased or decreased from 27°C to 40°C or to -4°C (0.15°C/min) at 0800 and 2000 (daytime and nighttime, respectively). Intra-abdominal temperature (Tcore) was monitored by telemetry. The relationship between Tcore and Ta was assessed. The range of Ta where Tcore was relatively stable (range of normothermia, RNT) and Tcore corresponding to the RNT median (regulated Tcore) were estimated by model analysis. In fed mice, the regression slope within the RNT was smaller in the nighttime than in the daytime (0.02 and 0.06, respectively), and the regulated Tcore was higher in the nighttime than in the daytime (37.5°C and 36.0°C, respectively). In the fasted mice, the slope remained unchanged, and the regulated Tcore decreased in the nighttime (0.05 and 35.9°C, respectively), while the slopes in the daytime became greater (0.13). Without the estimating individual thermoregulatory response such as metabolic heat production and skin vasodilation, the analysis of the Ta-Tcore relationship could describe the character of the core temperature control. The present results show that the character of the system changes depending on time of day and feeding conditions.

    DOI PubMed

  • Daily exercise may alter anti-oxidative responses to heat in mouse brain

    Kei Nagashima, Ken Tokizawa, Chisa Obata, Mizuki Tsunakawa, Shuri Marui

    FASEB JOURNAL   29  2015.04  [Refereed]

  • Deprivation of plasma estradiol level affects circadian rhythms of body core and tail skin temperature in female rats

    Shuri Marui, Kei Nagashima

    FASEB JOURNAL   29  2015.04  [Refereed]

  • Characteristics of activated neurons in the suprachiasmatic nucleus when mice become hypothermic during fasting and cold exposure.

    Yuki Uchida, Ken Tokizawa, Kei Nagashima

    Neuroscience letters   579   177 - 82  2014.09  [Refereed]  [International journal]

     View Summary

    Cold defense mechanisms in mice are attenuated in the light phase during fasting, resulting in hypothermia. The present study examined whether specific neurons and areas in the SCN are related to the response. Mice were fasted over 47h or remained fed, during which they were placed at 20 or 27°C for 3h in the light or dark phases. Body temperature (Tb) was monitored. After the exposure, immunoreactive (IR) cells of cFos, arginine vasopressin (AVP), and vasoactive intestinal polypeptide (VIP) in the SCN were assessed. Tb at 20°C during fasting was lower in the light phase than in the dark phase. Both AVP/cFos-IR and VIP/cFos-IR cells increased when mice were at 20°C during fasting in the light phase. Such increase was observed in the central part of the SCN. These responses in the SCN may be related to the hypothermia in the light phase.

    DOI PubMed

  • 暑熱適応における恒常性の維持と破綻

    永島計

    体育の科学   64 ( 7 ) 456 - 460  2014.07

  • 水分補給

    永島計

    Tarzan   29 ( 14 ) 96 - 99  2014.07

  • The influence of estrogen on circadian rhythms of heart rates and cardiac sympathetic nerve in female rats

    Shuri Marui, Mayumi Matsuda, Nobuo Sato, Kei Nagashima

    FASEB JOURNAL   28 ( 1 )  2014.04  [Refereed]

  • Protection of the brain against heat damage

    Mayumi Matsuda-Nakamura, Kei Nagashima

    The Journal of Physical Fitness and Sports Medicine   3 ( 2 ) 217 - 221  2014.03  [Refereed]

    DOI

  • 高体温症,低体温症の病体生理

    永島 計

    小児内科   46 ( 3 ) 310 - 314  2014.03

  • スポーツ中の熱中症を防ごう

    永島 計

    少年写真新聞、中学保健ニュース   1561   1 - 1  2013.05

  • The effect of spontaneous running-wheel exercise on behavioral thermoregulation in heat and thermal preference in mice : a possible role of the central

    Kei Nagashima, Lin Cheng-Hsien, Mayumi Matsuda

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S31 - S31  2013  [Refereed]

  • The effect of estrogen on biological rhythms in female rats

    Shuri Marui, Mayumi Matsuda, Nobuo Sato, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S158 - S158  2013  [Refereed]

  • The effect of feeding condition and ambient temperature to sleep-wake pattern

    Nobuo Sato, Matsuda Mayumi, Marui Shuri, Ozaki Makoto, Nagashima Kei

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S256  2013  [Refereed]

  • The effect of head cooling on body core temperature and thermoregulation in humans

    Mayumi Matsuda-Nakamura, Shuri Marui, Nobuo Sato, Lin Cheng-Hsien, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S283 - S283  2013  [Refereed]

  • Relative importance of different surface regions for thermal comfort in humans.

    Mayumi Nakamura, Tamae Yoda, Larry I Crawshaw, Momoko Kasuga, Yuki Uchida, Ken Tokizawa, Kei Nagashima, Kazuyuki Kanosue

    European journal of applied physiology   113 ( 1 ) 63 - 76  2013.01  [Refereed]  [International journal]

     View Summary

    In a previous study, we investigated the contribution of the surface of the face, chest, abdomen, and thigh to thermal comfort by applying local temperature stimulation during whole-body exposure to mild heat or cold. In hot conditions, humans prefer a cool face, and in cold they prefer a warm abdomen. In this study, we extended investigation of regional differences in thermal comfort to the neck, hand, soles, abdomen (Experiment 1), the upper and lower back, upper arm, and abdomen (Experiment 2). The methodology was similar to that used in the previous study. To compare the results of each experiment, we utilized the abdomen as the reference area in these experiments. Thermal comfort feelings were not particularly strong for the limbs and extremities, in spite of the fact that changes in skin temperature induced by local temperature stimulation of the limbs and extremities were always larger than changes that were induced in the more proximal body parts. For the trunk areas, a significant difference in thermal comfort was not observed among the abdomen, and upper and lower back. An exception involved local cooling during whole-body mild cold exposure, wherein the most dominant preference was for a warmer temperature of the abdomen. As for the neck and abdomen, clear differences were observed during local cooling, while no significant difference was observed during local warming. We combined the results for the current and the previous study, and characterized regional differences in thermal comfort and thermal preference for the whole-body surface.

    DOI PubMed

  • ヒトにおける体温調節の意味と意義

    永島 計, 中村真由美

    人間科学研究   26 ( 1 ) 13 - 25  2013.01

  • 体温と性差

    永島 計, 中村真由美

    体育の科学   62 ( 12 ) 928 - 933  2012.12

  • Ghrelin induces time-dependent modulation of thermoregulation in the cold.

    Ken Tokizawa, Yuki Onoue, Yuki Uchida, Kei Nagashima

    Chronobiology international   29 ( 6 ) 736 - 46  2012.07  [Refereed]  [International journal]

     View Summary

    Fasted mice show torpor-like hypothermia in the cold in their inactive phase. The aim of the present study was to elucidate whether leptin and/or ghrelin are involved in this reaction and to identify its neurophysiological mechanisms. In ob/ob mice, which lack leptin, metabolic heat production (oxygen consumption, Vo(2)) was suppressed in 20°C cold in both the light and dark phases, resulting in hypothermia. When wild-type mice received a systemic injection of 8 µg ghrelin in the early light phase, followed by a 2-h cold exposure to 10°C, their core body temperature (T(b)) decreased by 1.7°C, and they displayed a less marked increase in Vo(2) compared with vehicle-injected mice. However, ghrelin injection in the early dark phase resulted in the maintenance of T(b) and increased Vo(2) in the mice, which was similar to the result observed in the vehicle-injected mice. The number of doubly labeled neurons with cFos and neuropeptide Y (NPY) in the suprachiasmatic nucleus was greater in the light phase in the ghrelin-injected mice, which may suggest that ghrelin activates NPY neurons. On the contrary, in the paraventricular nucleus, the counts became greater only when they were exposed to the cold in the dark phase. These results indicate that ghrelin plays an important role in inducing time-dependent changes in thermoregulation in the cold via hypothalamic pathways.

    DOI PubMed

  • Hypothalamic mechanisms relating to improvement of thermal tolerance in heat-and-exercise-acclimated mice

    Ken Tokizawa, Chen-Hsien Lin, Yuki Uchida, Shin-ichi Sakakibara, Kei Nagashima

    FASEB JOURNAL   26  2012.04  [Refereed]

  • Increased daily-activity alters thermal perception and behavioral thermoregulation in mice

    Cheng-Hsien Lin, Ken Tokizawa, Yuki Uchida, Kei Nagashima

    FASEB JOURNAL   26  2012.04  [Refereed]

  • ヒトにおける体温の意味と意義

    永島 計, 松田(中村)真由美

    人間科学研究   25 ( 1 ) 21 - 34  2012.03

  • Tail position affects the body temperature of rats during cold exposure in a low-energy state.

    Yuki Uchida, Ken Tokizawa, Mayumi Nakamura, Cheng-Hsien Lin, Kei Nagashima

    Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology   198 ( 2 ) 89 - 95  2012.02  [Refereed]  [International journal]

     View Summary

    Rats place their tails underneath their body trunks when cold (tail-hiding behavior). The aim of the present study was to determine whether this behavior is necessary to maintain body temperature. Male Wistar rats were divided into 'fed' and '42-h fasting' groups. A one-piece tail holder (8.4 cm in length) that prevented the tail-hiding behavior or a three-piece tail holder (2.8 cm in length) that allowed for the tail-hiding behavior was attached to the tails of the rats. The rats were exposed to 27°C for 180 min or to 20°C for 90 min followed by 15°C for 90 min with continuous body temperature and oxygen consumption measurements. Body temperature decreased by -1.0 ± 0.1°C at 15°C only in the rats that prevented tail-hiding behavior of the 42-h fasting group, and oxygen consumption increased at 15°C in all animals. Oxygen consumption was not different between the rats that prevented tail-hiding behavior and the rats that allowed the behavior in the fed and 42-h fasting groups under ambient conditions. These results show that the tail-hiding behavior is involved in thermoregulation in the cold in fasting rats.

    DOI PubMed

  • Mechanisms of heat acclimation and tolerance induced by exercise training and heat exposure

    Tokizawa K, Lin CH, Nagashima, K

    J Phys Fitness Sports Med   1 ( 2 ) 343 - 346  2012.02  [Refereed]

    DOI

  • Hyperosmolality in the plasma modulates behavioral thermoregulation in mice: the quantitative and multilateral assessment using a new experimental system.

    Cheng-Hsien Lin, Ken Tokizawa, Mayumi Nakamura, Yuki Uchida, Hisae Mori, Kei Nagashima

    Physiology & behavior   105 ( 2 ) 536 - 43  2012.01  [Refereed]  [International journal]

     View Summary

    We evaluated the effect of plasma hyperosmolality on behavioral thermoregulation in mice, using a new experimental system. The system consisted of Plexiglas box (dimensions: 50×12×19 cm) with five computer-controlled Peltier boards (dimensions: 10×10 cm) at the bottom. Experiments were conducted in two different settings of the system. An operant behavior setting: each board was first set to 39°C, and the right-end board was changed to 20°C for 1 min when a mouse moved to a specific position. A temperature mosaic setting: each board was randomly set to 15°C, 22°C, 28°C, 35°C, or 39°C with a 6-min interval, but each board temperature was different from the others at a given time point. Mice were injected subcutaneous (s.c.) isotonic or hypertonic saline (154 mM (IS group) or 2,500 mM (HS group), 10 ml/kg body wt), and exposed to either setting for 90 min. In the operant setting, the HS group showed fewer operant behavior counts than the IS group (11±5 and 25±4 counts, respectively; P<0.05) with greater increase in body temperature (1.6±0.4°C vs. 0.0±0.2°C, respectively; P<0.05). In the mosaic setting, the HS group selected the board temperature of 35°C more frequently than the other temperatures (P<0.05) with the same increase in body temperature. These results may suggest that plasma hyperosmolality modulates behavioral thermoregulatory response to heat and induce regulated hyperthermia.

    DOI PubMed

  • 周術期の低体温:呼吸・循環への低体温の影響

    永島 計, 時澤 健, 内田有希

    LISA   19 ( 1 ) 14 - 17  2012.01

  • Exercise and thermoregulation

    Nagashima, Kei Tokizawa, Ken Uchida, Yuki Nakamura-Matsuda, Mayumi Lin, Chen-Hsien

    The Journal of Physical Fitness and Sports Medicine   1 ( 1 ) 73 - 82  2012.01  [Refereed]

    DOI

  • Intragastric administration of capsiate, a transient receptor potential channel agonist, triggers thermogenic sympathetic responses.

    Kaori Ono, Masako Tsukamoto-Yasui, Yoshiko Hara-Kimura, Naohiko Inoue, Yoshihito Nogusa, Yuki Okabe, Kei Nagashima, Fusao Kato

    Journal of applied physiology (Bethesda, Md. : 1985)   110 ( 3 ) 789 - 98  2011.03  [Refereed]  [International journal]

     View Summary

    The sympathetic thermoregulatory system controls the magnitude of adaptive thermogenesis in correspondence with the environmental temperature or the state of energy intake and plays a key role in determining the resultant energy storage. However, the nature of the trigger initiating this reflex arc remains to be determined. Here, using capsiate, a digestion-vulnerable capsaicin analog, we examined the involvement of specific activation of transient receptor potential (TRP) channels within the gastrointestinal tract in the thermogenic sympathetic system by measuring the efferent activity of the postganglionic sympathetic nerve innervating brown adipose tissue (BAT) in anesthetized rats. Intragastric administration of capsiate resulted in a time- and dose-dependent increase in integrated BAT sympathetic nerve activity (SNA) over 180 min, which was characterized by an emergence of sporadic high-activity phases composed of low-frequency bursts. This increase in BAT SNA was abolished by blockade of TRP channels as well as of sympathetic ganglionic transmission and was inhibited by ablation of the gastrointestinal vagus nerve. The activation of SNA was delimited to BAT and did not occur in the heart or pancreas. These results point to a neural pathway enabling the selective activation of the central network regulating the BAT SNA in response to a specific stimulation of gastrointestinal TRP channels and offer important implications for understanding the dietary-dependent regulation of energy metabolism and control of obesity.

    DOI PubMed

  • Regional difference of cFos expression in the preoptic areas of the hypothalamus during heat and cold in mice

    Yuki Uchida, Keisuke Onishi, Mayumi Nakamura, Ken Tokizawa, Kei Nagashima

    NEUROSCIENCE RESEARCH   71   E159 - E159  2011  [Refereed]

    DOI

  • Ghrelin as a regulator of the circadian body temperature rhythm

    Kei Nagashima, Ken Tokizawa

    NEUROSCIENCE RESEARCH   71   E169 - E169  2011  [Refereed]

    DOI

  • A new apparatus assessing thermal preference in mice

    Cheng-Hsien Lin, Ken Tokizawa, Yuki Uchida, Kei Nagashima

    NEUROSCIENCE RESEARCH   71   E368 - E368  2011  [Refereed]

    DOI

  • Estrogen in the medial preoptic nucleus of the hypothalamus modulates cold responses in female rats.

    Yuki Uchida, Ken Tokizawa, Mayumi Nakamura, Hisae Mori, Kei Nagashima

    Brain research   1339   49 - 59  2010.06  [Refereed]  [International journal]

     View Summary

    The present study examined the effect of the central administration of estrogen on responses to the cold. Estrogen or cholesterol was applied locally to the medial preoptic nucleus (MPO) or dorsomedial hypothalamic nucleus (DMH) of the hypothalamus in free-moving ovariectomized rats. Forty-eight hours after the application, rats had 2-h exposure at 10 or 25 degrees C. Body temperature (T(b)) and the tail surface temperature (T(tail)) were continuously measured by telemetry and thermography, respectively. The change of T(b) at 10 degrees C from the 25 degrees C baseline was higher in the estrogen application in the MPO than that in the cholesterol application; however, such difference was not observed in the DMH application. The uncoupling 1 protein mRNA level in the interscapular brown adipose tissue involved in non-shivering thermogenesis was not different between the estrogen and cholesterol applications in the MPO and DMH. T(tail) decreased in the cold, which was greater after the estrogen application in the MPO than after the cholesterol application. These results show that estrogen affects the MPO in female rats, changing T(b) in the cold. Moreover, suppression of heat loss from the tail may be involved in the mechanism.

    DOI PubMed

  • Estrogen in the medial preoptic nucleus of the hypothalamus modulates cold responses in female rats.

    Uchida Yuki, Tokizawa Ken, Nakamura Mayumi, Mori Hisae, Nagashima Kei

    Estrogen in the medial preoptic nucleus of the hypothalamus modulates cold responses in female rats.   1339   49 - 59  2010.06  [Refereed]

     View Summary

    :The present study examined the effect of the central administration of estrogen on responses to the cold. Estrogen or cholesterol was applied locally to the medial preoptic nucleus (MPO) or dorsomedial hypothalamic nucleus (DMH) of the hypothalamus in free-moving ovariectomized rats. Forty-eight hours after the application, rats had 2-h exposure at 10 or 25 degrees C. Body temperature (T(b)) and the tail surface temperature (T(tail)) were continuously measured by telemetry and thermography, respectively. The change of T(b) at 10 degrees C from the 25 degrees C baseline was higher in the estrogen application in the MPO than that in the cholesterol application; however, such difference was not observed in the DMH application. The uncoupling 1 protein mRNA level in the interscapular brown adipose tissue involved in non-shivering thermogenesis was not different between the estrogen and cholesterol applications in the MPO and DMH. T(tail) decreased in the cold, which was greater after the estrogen application in the MPO than after the cholesterol application. These results show that estrogen affects the MPO in female rats, changing T(b) in the cold. Moreover, suppression of heat loss f

    DOI PubMed

  • Mild hypohydration induced by exercise in the heat attenuates autonomic thermoregulatory responses to the heat, but not thermal pleasantness in humans.

    Ken Tokizawa, Saki Yasuhara, Mayumi Nakamura, Yuki Uchida, Larry I Crawshaw, Kei Nagashima

    Physiology & behavior   100 ( 4 ) 340 - 5  2010.06  [Refereed]  [International journal]

     View Summary

    Hypohydration caused by exercise in the heat attenuates autonomic thermoregulation such as sweating and skin blood flow in humans. In contrast, it remains unknown if behavioral thermoregulation is modulated during hypohydration. We assume that thermal unpleasantness could drive the behavioral response, and would also be modulated during hypohydration. Nine healthy young men participated in the present study. Body and skin temperatures were monitored. Ratings of thermal sensation and pleasantness were conducted. After approximately 45 min rest at 27 degrees C, they performed 50-min cycling exercise, which was at the level of 40% of heart rate range at 35 degrees C (hypohydration trial) or at the level of 10% of heart rate range at 23 degrees C (control trial), respectively. Subjects returned to the rest at 27 degrees C, and the ambient temperature was then changed from 22 to 38 degrees C. Body weight decreased by 0.9+/-0.1% immediately after exercise in the hypohydration trial and 0.3+/-0.1% in the control trial. In the cold, no significant difference in thermal sensation or pleasantness was observed between trials. There was no significant difference in thermal pleasantness between trials in the heat, although thermal sensation in the heat (32.5-36 degrees C) was significantly lower in the hypohydration trial than in the control trial. In addition, laser Doppler flow of the skin and sweat rate were attenuated in the heat in the hypohydration trial. These results may indicate that mild hypohydration after exercise in the heat has no influence on behavioral responses to the heat.

    DOI PubMed

  • Concepts to utilize in describing thermoregulation and neurophysiological evidence for how the system works.

    Kazuyuki Kanosue, Larry I Crawshaw, Kei Nagashima, Tamae Yoda

    European journal of applied physiology   109 ( 1 ) 5 - 11  2010.05  [Refereed]  [International journal]

     View Summary

    We would like to emphasize about the system involved with homeostatic maintenance of body temperature. First, the primary mission of the thermoregulatory system is to defend core temperature (T (core)) against changes in ambient temperature (T (a)), the most frequently encountered disturbance for the system. T (a) should be treated as a feedforward input to the system, which has not been adequately recognized by thermal physiologists. Second, homeostatic demands from outside the thermoregulatory system may require or produce an altered T (core), such as fever (demand from the immune system). There are also conditions where some thermoregulatory effectors might be better not recruited due to demands from other homeostatic systems, such as during dehydration or fasting. Third, many experiments have supported the original assertion of Satinoff that multiple thermoregulatory effectors are controlled by different and relatively independent neuronal circuits. However, it would also be of value to be able to characterize strictly regulatory properties of the entire system by providing a clear definition for the level of regulation. Based on the assumption that T (core) is the regulated variable of the thermoregulatory system, regulated T (core) is defined as the T (core) that pertains within the range of normothermic T (a) (Gordon in temperature and toxicology: an integrative, comparative, and environmental approach, CRC Press, Boca Raton, 2005), i.e., the T (a) range in which an animal maintains a stable T (core). The proposed approach would facilitate the categorization and evaluation of how normal biological alterations, physiological stressors, and pathological conditions modify temperature regulation. In any case, of overriding importance is to recognize the means by which an alteration in T (core) (and modification of associated effector activities) increases the overall viability of the organism.

    DOI PubMed

  • Estrogen modulates central and peripheral responses to cold in female rats.

    Yuki Uchida, Masumi Kano, Saki Yasuhara, Akiko Kobayashi, Ken Tokizawa, Kei Nagashima

    The journal of physiological sciences : JPS   60 ( 2 ) 151 - 60  2010.03  [Refereed]  [Domestic journal]

     View Summary

    The aim of this study was to determine whether estrogen modulates central and peripheral responses to cold in female rats. In ovariectomized female rats with and without administered estrogen [E(2) (+) and E(2) (-), respectively], the counts of cFos-immunoreactive cells in the medial preoptic nucleus (MPO) and dorsomedial hypothalamic nucleus (DMH) in the hypothalamus were greater in the E(2) (+) rats than in the E(2) (-) rats at 5 degrees C. Examination of the response of normal female rats to exposure to 5 degrees C at different phases of the estrus cycle revealed that counts of cFos-immunoreactive cells in the MPO, DMH, and posterior hypothalamus and the level of uncoupling protein 1 mRNA in the brown adipose tissues were greater in the proestrus phase than on day 1 of the diestrus phase. This result was linked to the level of plasma estrogen. The body temperature during cold exposure was higher in the E(2) (+) rats than in the E(2) (-) rats and was also higher in the proestrus phase than on day 1 of the diestrus phase. We conclude that estrogen may affect central and peripheral responses involved in thermoregulation in the cold.

    DOI PubMed

  • New thermoregulatory behavior in rats in a cold environment

    Yuki Uchida, Ken Tokizawa, Mayumi Nakamura, Hisae Mori, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60   S101 - S101  2010  [Refereed]

  • Ghrelin affects thermoregulation and hypothalamic cFos expressions in the cold, depending on time of the day

    Ken Tokizawa, Yuki Onoue, Yuki Uchida, Hisae Mori, Mayumi Nakamura, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60   S101 - S101  2010  [Refereed]

  • New system for estimation of behavioral thermoregulation in mice

    Hisae Mori, Yuki Uchida, Mayumi Nakamura, Ken Tokizawa, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   60   S190 - S190  2010  [Refereed]

  • Ghrelin plays an important role in time-dependent change in thermoregulation during cold in mice

    Ken Tokizawa, Yuki Onoue, Yuki Uchida, Hisae Mori, Mayumi Nakamura, Kei Nagashima

    NEUROSCIENCE RESEARCH   68   E283 - E283  2010  [Refereed]

    DOI

  • Thermoregulation in the cold changes depending on the time of day and feeding condition: physiological and anatomical analyses of involved circadian mechanisms

    K. Tokizawa, Y. Uchida, K. Nagashima

    Neuroscience   164 ( 3 ) 1377 - 1386  2009.12  [Refereed]

     View Summary

    The circadian rhythm of body temperature (Tb) is a well-known phenomenon. However, it is unknown how the circadian system including the suprachiasmatic nucleus (SCN) and clock genes affects thermoregulation. Food deprivation in mice induces a greater reduction of Tb particularly in the light phase. We examined the role of Clock, one of key clock genes and the SCN during induced hypothermia. At 20 °C with fasting, mice increased their metabolic heat production in the dark phase and maintained Tb, whereas in the light phase, heat production was less, resulting in hypothermia. Under these conditions, neuronal activity in the SCN, assessed by cFos expression, increased only in the light phase. However, such differences in thermoregulatory and neural responses between the phases in Clock mutant mice were less marked. The neural network between the SCN and paraventricular nucleus appeared to be important in hypothermia. These findings suggest that the circadian system per se is influenced by both the feeding condition and environmental temperature and that it modulates thermoregulation. © 2009 IBRO.

    DOI PubMed

  • Clock gene plays an important role in time-dependent change in thermoregulation during cold exposure in fasted mice

    Ken Tokizawa, Yuki Uchida, Kei Nagashima

    FASEB JOURNAL   23  2009.04  [Refereed]

  • Administration of 17-beta estradiol in the medial preoptic area prevents reduction of body temperature during cold exposure in ovariectomized rats

    Yuki Uchida, Madoka Tohi, Ken Tokizawa, Kei Nagashima

    FASEB JOURNAL   23  2009.04  [Refereed]

  • ADMINISTRATION OF 17-beta ESTRADIOL IN THE MEDIAL PREOPTIC AREA SUPPRESSES HEAT DISSIPATION DURING COLD EXPOSURE IN OVARIECTOMIZED RATS

    Yuki Uchida, Madoka Tohi, Ken Tokizawa, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   166 - 166  2009  [Refereed]

  • REGIONAL DIFFERENCES IN THERMAL COMFORT IN HUMANS

    Mayumi Nakamura, Tamae Yoda, Yuki Uchida, Ken Tokizawa, Kei Nagashima, Kazuyuki Kanosue

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   205 - 205  2009  [Refereed]

  • GASTROINTESTINAL TRPV1 ACTIVATION ENHANCES SYMPATHETIC OUTFLOW TO BROWN ADIPOSE TISSUE

    Masako Yasui, Kaori Ono, Yoshiko Kimura, Naohiko Inoue, Yoshihito Nogusa, Yuki Okabe, Kei Nagashima, Fusao Kato

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   474 - 474  2009  [Refereed]

  • ROLE OF THE MEDIAN PREOPTIC NUCLEUS IN THERMOREGULATION DURING LOCAL BRAIN HEATING WITH OSMOTIC STRESS

    Kei Nagashima, Yuki Uchida, Akiko Kobayashi, Ken Tokizawa

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   474 - 474  2009  [Refereed]

  • TIME-DEPENDENT CHANGES IN THERMOREGULATION DURING COLD EXPOSURE IN FASTED MICE

    Ken Tokizawa, Yuki Uchida, Kei Nagashima

    JOURNAL OF PHYSIOLOGICAL SCIENCES   59   535 - 535  2009  [Refereed]

  • Regional differences in temperature sensation and thermal comfort in humans.

    Mayumi Nakamura, Tamae Yoda, Larry I Crawshaw, Saki Yasuhara, Yasuyo Saito, Momoko Kasuga, Kei Nagashima, Kazuyuki Kanosue

    Journal of applied physiology (Bethesda, Md. : 1985)   105 ( 6 ) 1897 - 906  2008.12  [Refereed]  [International journal]

     View Summary

    Sensations evoked by thermal stimulation (temperature-related sensations) can be divided into two categories, "temperature sensation" and "thermal comfort." Although several studies have investigated regional differences in temperature sensation, less is known about the sensitivity differences in thermal comfort for the various body regions. In the present study, we examined regional differences in temperature-related sensations with special attention to thermal comfort. Healthy male subjects sitting in an environment of mild heat or cold were locally cooled or warmed with water-perfused stimulators. Areas stimulated were the face, chest, abdomen, and thigh. Temperature sensation and thermal comfort of the stimulated areas were reported by the subjects, as was whole body thermal comfort. During mild heat exposure, facial cooling was most comfortable and facial warming was most uncomfortable. On the other hand, during mild cold exposure, neither warming nor cooling of the face had a major effect. The chest and abdomen had characteristics opposite to those of the face. Local warming of the chest and abdomen did produce a strong comfort sensation during whole body cold exposure. The thermal comfort seen in this study suggests that if given the chance, humans would preferentially cool the head in the heat, and they would maintain the warmth of the trunk areas in the cold. The qualitative differences seen in thermal comfort for the various areas cannot be explained solely by the density or properties of the peripheral thermal receptors and thus must reflect processing mechanisms in the central nervous system.

    DOI PubMed

  • Capsinoids, TRPV1 Agonists, Enhance Sympathetic Outflow to Brown Adipose Tissue

    Masako Yasui, Kaori Ono, Yoshiko Hara, Naohiko Inoue, Yoshihito Nogusa, Yuki Okabe, Kei Nagashima, Fusao Kato

    OBESITY   16   S191 - S191  2008.10  [Refereed]

  • Effects of alcohol on autonomic responses and thermal sensation during cold exposure in humans.

    Tamae Yoda, Larry I Crawshaw, Kumiko Saito, Mayumi Nakamura, Kei Nagashima, Kazuyuki Kanosue

    Alcohol (Fayetteville, N.Y.)   42 ( 3 ) 207 - 12  2008.05  [Refereed]  [International journal]

     View Summary

    We investigated the effects of alcohol on thermoregulatory responses and thermal sensations during cold exposure in humans. Eight healthy men (mean age 22.3+/-0.7 year) participated in this study. Experiments were conducted twice for each subject at a room temperature of 18 degrees C. After a 30-min resting period, the subject drank either 15% alcohol at a dose of 0.36 g/kg body weight (alcohol session) or an equal volume of distilled water (control session), and remained in a sitting position for another 60 min. Mean skin temperature continued to decrease and was similar in control and alcohol sessions. Metabolic rate was lower in the alcohol session, but the difference did not affect core temperature, which decreased in a similar manner in both alcohol and control sessions (from 36.9+/-0.1 degrees C to 36.6+/-0.1 degrees C). Whole body sensations of cold and thermal discomfort became successively stronger in the control session, whereas these sensations were both greatly diminished after drinking alcohol. In a previous study we performed in the heat, using a similar protocol, alcohol produced a definite, coordinated effect on all autonomic and sentient heat loss effectors. In the current study in the cold, as compared to responses in the heat, alcohol intake was followed by lesser alterations in autonomic effector responses, but increased changes in sensations of temperature and thermal discomfort. Overall, our results indicate that although alcohol influences thermoregulation in the cold as well as in the heat, detailed aspects of the influence are quite different.

    DOI PubMed

  • Fasting affects thermoregulation in the cold, depending on time of the day

    Ken Tokizawa, Yuki Uchida, Kei Nagashima

    FASEB JOURNAL   22  2008.04  [Refereed]

  • Effect of central application of 17-beta estradiol on thermoregulation during a cold exposure in female rats

    Yuki Uchida, Ken Tokizawa, Kei Nagashima

    FASEB JOURNAL   22  2008.04  [Refereed]

  • The influence of exercise-induced dehydration on thermal sensation and comfort

    Kei Nagashima, Ken Tokizawa, Saki Yasuhara, Mayumi Nakamura, Larry Crawshaw

    FASEB JOURNAL   22   103 - 103  2008.04  [Refereed]

     View Summary

    Introduction Dehydration attenuates autonomic thermoregulation in the heat such as sweating and skin blood flow in human. Hyperosmolality in the extracellular fluid is thought to be involved in this mechanism. In contrast, behavioral thermoregulation (i.e. heat escape behavior) is augmented after hypertonic saline injection in rats (Nagashima et al., Am J Physiol 2001). However, it remains unknown if human activate behavioral thermoregulation during dehydration. Methods Ten healthy young men participated in the present study. Body and skin temperature were monitored. Rating for thermal sensation and comfort were conducted. After 45 min rest at 27&deg;C, they did 50 min ergometer exercise, which was at the level of 40% peak VO2 or 15% peak VO2 at an ambient temperature of 35&deg;C or 24&deg;C, respectively. Subjects returned to the rest at 27&deg;C until body and skin temperature being restored. Ambient temperature was then changed from 22&deg;C to 38&deg;C. Results Body weight decreased by 1.1 &plusmn; 0.1% after the exercise at 35&deg;C and 0.2 &plusmn; 0.1% at 24&deg;C. Plasma osmolality increased by 6 &plusmn; 1 mosmol/kg H2O at 35&deg;C, but did not change at &sim;24&deg;C. Thermal sensation and comfort in the heat were augmented (i.e. feeling hotter and more uncomfortable) after the exercise at 35&deg;C. Conclusion Dehydration increases thermal sensation and comfort in the heat in human, which are closely related to behavioral thermoregulation. [J Physiol Sci. 2008;58 Suppl:S103]

    DOI CiNii

  • The impact of estrogen on thermoregulatory response to the cold in female rats

    Kei Nagashima, Masumi Kano, Yuki Uchida, Ken Tokizawa

    NEUROSCIENCE RESEARCH   61   S63 - S63  2008  [Refereed]

  • Effect of hypothalamic application of 17-beta estradiol on thermoregulation in a cold environment

    Yuki Uchida, Ken Tokizawa, Masumi Kano, Kei Nagashima

    NEUROSCIENCE RESEARCH   61   S186 - S186  2008  [Refereed]

  • Fasting affects thermoregulation and hypothalamic Fos expressions in the cold, depending an time of the day

    Ken Tokizawa, Yuki Uchida, Kei Nagashima

    NEUROSCIENCE RESEARCH   61   S254 - S254  2008  [Refereed]

  • The role of estrogen in thermoregulation in female rats

    Kei Nagashima

    Autonomic Neuroscience   135 ( 1-2 ) 26  2007.09  [Refereed]

    DOI

  • Effects of posture on peripheral vascular responses to lower body positive pressure.

    Takeshi Nishiyasu, Shigeko Hayashida, Asami Kitano, Kei Nagashima, Masashi Ichinose

    American journal of physiology. Heart and circulatory physiology   293 ( 1 ) H670-6 - H676  2007.07  [Refereed]  [International journal]

     View Summary

    We tested the hypothesis that peripheral vascular responses (in the lower and upper limbs) to application of lower body positive pressure (LBPP) are dependent on the posture of the subjects. We measured heart rate, stroke volume, mean arterial pressure, leg and forearm blood flow (using the Doppler ultrasound technique), and leg (LVC) and forearm (FVC) vascular conductance in 11 subjects (9 men, 2 women) without and with LBPP (25 and 50 mmHg) in supine and upright postures. Mean arterial pressure increased in proportion to increases in LBPP and was greater in supine than in upright subjects. Heart rate was unchanged when LBPP was applied to supine subjects but was reduced in upright ones. Leg blood flow and LVC were both reduced by LBPP in supine subjects [LVC: 4.8 (SD 4.0), 3.6 (SD 3.5), and 1.4 (SD 1.8) ml.min(-1).mmHg(-1) before LBPP and during 25 and 50 mmHg LBPP, respectively; P < 0.05] but were increased in upright ones [LVC: 2.0 (SD 1.2), 3.4 (SD 3.4), and 3.0 (SD 2.0) ml.min(-1).mmHg(-1), respectively; P < 0.05]. Forearm blood flow and FVC both declined when LBPP was applied to supine subjects [FVC: 1.3 (SD 0.6), 1.0 (SD 0.4), and 0.9 (SD 0.6) ml. min(-1).mmHg(-1), respectively; P < 0.05] but remained unchanged in upright ones [FVC: 0.7 (SD 0.4), 0.7 (SD 0.4), and 0.6 (SD 0.5) ml.min(-1).mmHg(-1), respectively]. Together, these findings indicate that the leg vascular response to application of LBPP is posture dependent and that the response differs in the lower and upper limbs when subjects assume an upright posture.

    DOI PubMed

  • The regional difference in temperature related sensations

    Mayumi Nakamura, Tamae Yoda, Saki Yasuhara, Yasuyo Saito, Momoko Kasuga, Kei Nagashima, Larry I. Crawshaw, Kazuyuki Kanosue

    NEUROSCIENCE RESEARCH   58   S108 - S108  2007  [Refereed]

    DOI

  • Capsiate enhances sympathetic nerve activity of brown adipose tissue

    Masako Yasui, Kaori Ono, Yoshiko Hara, Kai Nagashima, Michio Takahashi

    NEUROSCIENCE RESEARCH   58   S105 - S105  2007  [Refereed]

    DOI

  • Physiological role of sex hormones and biological clock in thermoregulation in rodents

    Kei Nagashima, Masumi Kano, Yuki Uchida

    NEUROSCIENCE RESEARCH   58   S163 - S163  2007  [Refereed]

    DOI

  • Menstrual cycle affects thermal regulation and comfort

    Saki Yasuhara, Aine Iwama, Masumi Kanou, Mayumi Nakamura, Kei Nagashima

    NEUROSCIENCE RESEARCH   58   S166 - S166  2007  [Refereed]

     View Summary

    INTRODUCTION It is well known that body temperature in female is closely linked with menstrual cycle. We reported that estrogen affects thermal regulation in rats: 1) estrogen is involved in circadian body temperature rhythm; 2) estrogen has an important role in thermal regulation in both the heat and cold. Moreover, in human beings, not a small population of females experiences some troubles in thermoregulation such as hot flash or cold sensation. Estrogen replacement therapy is sometimes effective for the females. In this study, we hypothesized that there would be differences in thermoregulation and thermal sensation, depending of menstruation cycle. METHOSDS and RESULTS Eight female subjects with a regular menstruation participated in this study. Experiments were conducted twice for each subject in follicular (F) and luteal (L) phases, determined by basal body temperature. Experimental condition was 120-min exposure at 23.5&deg;C after a 40-min baseline at 29.5&deg;C. Mean skin temperature decreased and body core temperature increased in both F and L during the 23.5&deg;C exposure. Oxygen consumption also increased in F and L. Based on rating scores for thermal sensation and comfort, L enhanced both coldness and unpleasantness when ambient temperature changed from 29.5&deg;C to 23.5&deg;C. COMCLUSION Although the autonomic thermal regulation seems to be similar between F and L, the menstrual phase may have an influence on thermal sensation or comfort. Estrogen may be involved in this mechanism. [J Physiol Sci. 2007;57 Suppl:S183]

    DOI CiNii

  • The median preoptic nucleus is involved in the facilitation of heat-escape/cold-seeking behavior during systemic salt loading in rats.

    Masahiro Konishi, Kazuyuki Kanosue, Masumi Kano, Akiko Kobayashi, Kei Nagashima

    American journal of physiology. Regulatory, integrative and comparative physiology   292 ( 1 ) R150-9 - R159  2007.01  [Refereed]  [International journal]

     View Summary

    Systemic salt loading has been reported to facilitate operant heat-escape/cold-seeking behavior. In the present study, we hypothesized that the median preoptic nucleus (MnPO) would be involved in this mechanism. Rats were divided into two groups (n = 6 each): one group had the MnPO lesion with ibotenic acid (4.0 mug) and the other was the vehicle control. After subcutaneous injection (10 ml/kg) of either isotonic- (154 mM) or hypertonic-saline (2,500 mM), each rat was placed in a behavior box, where the ambient temperature was changed to 26 degrees C, 35 degrees C, and 40 degrees C every 1 h. The position of a rat in the box and the body core temperature (T(core)) were monitored. A rat could trigger 0 degrees C air for 45 s in the 35 degrees C and 40 degrees C heat when moved in a specific area in the box (operant behavior). In the control group, counts of the operant behavior were greater (P < 0.05) in the hypertonic- than in the isotonic-saline injection (17 +/- 2 and 10 +/- 2 at 35 degrees C, 24 +/- 2 and 18 +/- 1 at 40 degrees C). T(core) remained unchanged throughout the exposure, although the level was lower (P < 0.05) in the hypertonic- than in the isotonic-saline trial (36.6 +/- 0.2 degrees C and 37.4 +/- 0.1 degrees C at 26 degrees C and 36.9 +/- 0.2 degrees C and 37.4 +/- 0.1 degrees C at 40 degrees C, respectively). However, in the MnPO-lesion group, counts of the behavior were similar between the hypertonic- and isotonic-saline injection trials (10 +/- 2 and 8 +/- 1 at 35 degrees C, and 17 +/- 1 and 16 +/- 1 at 40 degrees C, respectively). T(core) increased (P < 0.05) in the heat in both trials (36.8 +/- 0.1 degrees C and 37.4 +/- 0.1 degrees C at 26 degrees C and 37.4 +/- 0.2 degrees C and 37.8 +/- 0.2 degrees C at 40 degrees C in the hypertonic- and isotonic-saline injection trials, respectively). These results may suggest that, at least in part, the MnPO is involved in the facilitation of heat-escape/cold-seeking behavior during osmotic stimulation.

    DOI PubMed

  • A new system for the analysis of thermal judgments: multipoint measurements of skin temperatures and temperature-related sensations and their joint visualization.

    Mayumi Nakamura, Hidenori Esaki, Tamae Yoda, Saki Yasuhara, Akiko Kobayashi, Aki Konishi, Naoki Osawa, Kei Nagashima, Larry I Crawshaw, Kazuyuki Kanosue

    The journal of physiological sciences : JPS   56 ( 6 ) 459 - 64  2006.12  [Refereed]  [Domestic journal]

     View Summary

    We report a new system for monitoring sensations of many body parts as well as comprehensively showing the distribution of overall skin temperature (T(sk)) and temperature-related sensations. The system consists of a console with 52 levers to report temperature-related sensations and software that facilitates the visualization of the distribution of T(sk) and temperature-related sensations by displaying them on a model of the human body. The system's utility was demonstrated with a physiological experiment involving three males and three females. They were exposed to step changes of ambient temperature from 23 degrees C to 33 degrees C. We measured T(sk) at 50 points, and the subjects concurrently provided estimates of local temperature sensation and thermal comfort/discomfort at 25 loci. This system greatly facilitates the perception and analysis of spatial relationships and differences in temperature and sensation in various areas of the body.

    DOI PubMed

  • Central mechanisms for thermoregulation in a hot environment.

    Kei Nagashima

    Industrial health   44 ( 3 ) 359 - 67  2006.07  [Refereed]  [Domestic journal]

     View Summary

    Homeothermic animals regulate body temperature by autonomic and behavioral thermoeffector responses. The regulation is conducted mainly in the brain. Especially, the preoptic area (PO) in the hypothalamus plays a key role. The PO has abundant warm-sensitive neurons, sending excitatory signals to the brain regions involved in heat loss mechanisms, and inhibitory signals to those involved in heat production mechanisms. The sympathetic fibers determine tail blood flow in rats, which is an effective heat loss process. Some areas in the midbrain and medulla are involved in the control of tail blood flow. Recent study also showed that the hypothalamus is involved in heat escape behavior in rats. However, our knowledge about behavioral regulation is limited. The central mechanism for thermal comfort and discomfort, which induce various behavioral responses, should be clarified. In the heat, dehydration affects both autonomic and behavioral thermoregulation by non-thermoregulatory factors such as high Na+ concentration. The PO seems to be closely involved in these responses. The knowledge about the central mechanisms involved in thermoregulation is important to improve industrial health, e.g. preventing accidents associated with the heat or organizing more comfortable working environment.

    DOI PubMed

  • Reflex activation of rat fusimotor neurons by body surface cooling, and its dependence on the medullary raphe.

    Mutsumi Tanaka, Neil C Owens, Kei Nagashima, Kazuyuki Kanosue, Robin M McAllen

    The Journal of physiology   572 ( Pt 2 ) 569 - 83  2006.04  [Refereed]  [International journal]

     View Summary

    The nature of muscle efferent fibre activation during whole body cooling was investigated in urethane-anaesthetized rats. Multiunit efferent activity to the gastrocnemius muscle was detected when the trunk skin was cooled by a water-perfused jacket to below 36.0 +/- 0.7 degrees C. That efferent activity was not blocked by hexamethonium (50 mg kg(-1), i.v.) and was not associated with movement or electromyographic activity. Cold-induced efferent activity enhanced the discharge of afferent filaments from the isotonically stretched gastrocnemius muscle, demonstrating that it was fusimotor. Fusimotor neurons were activated by falls in trunk skin temperature, but that activity ceased when the skin was rewarmed, regardless of how low core temperature had fallen. While low core temperature alone was ineffective, a high core temperature could inhibit the fusimotor response to skin cooling. Fusimotor activation by skin cooling was often accompanied by desynchronization of the frontal electroencephalogram (EEG), but was not a simple consequence of cortical arousal, in that warming the scrotum desynchronized the EEG without activating fusimotor fibres. Inhibition of neurons in the rostral medullary raphé by microinjections of glycine (0.5 m, 120-180 nl) reduced the fusimotor response to skin cooling by 95 +/- 3%, but did not prevent the EEG response. These results are interpreted as showing a novel thermoregulatory reflex that is triggered by cold exposure. It may underlie the increased muscle tone that precedes overt shivering, and could also serve to amplify shivering. Like several other cold-defence responses, this reflex depends upon neurons in the rostral medullary raphé.

    DOI PubMed

  • The role of biological clock in thermoregulation

    Kei Nagashima

    NEUROSCIENCE RESEARCH   55   S23 - S23  2006  [Refereed]

  • Effects of warming the lower back with a heat and steam generating sheet on thermoregulatory responses and sensation

    Oda, H, Igaki, M, Ugajin T, Suzuki, A, Tshuchiya S, Nagashima, K, Iso, S, Kanosue, K

    日本気誌   43 ( 1 ) 43 - 50  2006

     View Summary

    In this study, we developed a heat and steam generating (HSG) sheet that generates steam for several hours, and analyzed its effects on thermoregulatory responses and thermal sensation. In seven healthy male subjects the lumbar region (lower back) was warmed for four hours in a cool environment of 20&deg;C and 40%RH. For each subject, three experiments were performed: 1) warming the lower back with a HSG sheet (12&times;20 cm), 2) warming with a heat generating (HG) sheet without steam, and 3) no warming (control). The skin and subcutaneous temperatures in and around the warmed area, as well as rectal temperature were measured. The subjects reported whole body thermal comfort, and local sensations in the warmed area. While the temperature under the sheet was similar between the HSG and HG sheets (approximately 38.5&deg;C), skin temperature at 2 cm from the sheet was about 0.9&deg;C higher (p<0.05) for the HSG sheet than the HG sheet. The subjects reported less whole body coldness during the warming by the HSG sheet than the HG sheet. They also reported stronger and more spreading warm sensation in the warmed region for the HSG sheet than the HG sheet. In a separate experiment using an apparatus with a heat flux sensor, higher heat flux was observed for the HSG sheet than the HG sheet, even though both sheets gave the same surface temperature. These results suggest that warming the skin with steam is more efficient than warming it with dry air.<br>

    DOI CiNii

  • Effects of alcohol on thermoregulation during mild heat exposure in humans.

    Tamae Yoda, Larry I Crawshaw, Mayumi Nakamura, Kumiko Saito, Aki Konishi, Kei Nagashima, Sunao Uchida, Kazuyuki Kanosue

    Alcohol (Fayetteville, N.Y.)   36 ( 3 ) 195 - 200  2005.07  [Refereed]  [International journal]

     View Summary

    We investigated the effects of alcohol on thermoregulatory responses and thermal sensations during mild heat exposure in humans. Eight healthy men participated in this study. Experiments were conducted twice for each subject at a room temperature of 33 degrees C. After a 30-min resting period, the subject drank either 15% alcohol (alcohol session) at a dose of 0.36 g/kg body weight or equal volume of water (control session). Skin blood flow and chest sweat rate in the alcohol session significantly increased over those in controls 10 min after drinking. Deep body temperature in the alcohol session started to decrease 20 min after the onset of sweating and eventually fell 0.3 degrees C lower than in the controls. Whole body hot sensation transiently increased after alcohol drinking, whereas it changed little after water drinking. The increased "hot" sensation would presumably cause cool-seeking behavior, if permitted. Thus, alcohol influences thermoregulation so that body core temperature is lowered not only by automatic mechanisms (sweating and skin vasodilation) but also behaviorally. These results suggest that decreases in body temperature after alcohol drinking are not secondary to skin vasodilation, a well-known effect of alcohol, but rather result from a decrease in the regulated body temperature evidenced by the coordinated modulation of various effectors of thermoregulation and sensation.

    DOI PubMed

  • Brain activation by thermal stimulation in humans studied with fMRI

    T Yagishita, N Sadato, T Okada, A Taniguchi, M Konishi, K Nagashima, Y Yonekura, K Kanosue

    Environmental Ergonomics   3   17 - 20  2005  [Refereed]

     View Summary

    Sensations evoked by innocuous thermal stimulation can be divided into two categories. One is "temperature sensation" in a narrow sense, which is directed towards an object outside the body. The other is the "thermal comfort/discomfort" of the body that is important for thermoregulation. We recently reported rCBF changes in the amygdala which correlated with thermal comfort during whole body cooling (Kanosue et al., 2000). In the present study we investigated the region of brain that is activated by local thermal stimulation of the hand. Eight healthy subjects were recruited and gave written informed consent to participate in the study. Warm (39 degrees C) or cold (25 degrees C) stimuli was applied to the right or left hand for 30 s by using a water circulating tube that covered the whole hand. Each subject reported the magnitude of the stimulus intensity of temperature sensation using a scale from 1 (very cold) to 9 (very hot). All subjects reported hot or cold sensations and not pain. We examined the correlation between the rating scores and regional activity over the entire brain with a 3 Tesla MR imagers (VP, General Electrics, Milwaukee, US). Activation was observed in the contralateral secondary somatosensory cortex in response to both warm and cold stimulation of the hand. No activation was observed in the amygdala. This suggests that temperature sensation and thermal comfort might be generated by completely different structures of the brain.

    DOI DOI2

  • Brain activation by thermal stimulation in humans studied with fMRI

    T Yagishita, N Sadato, T Okada, A Taniguchi, M Konishi, K Nagashima, Y Yonekura, K Kanosue

    Environmental Ergonomics   3 ( C ) 17 - 20  2005  [Refereed]

     View Summary

    Sensations evoked by innocuous thermal stimulation can be divided into two categories. One is "temperature sensation" in a narrow sense, which is directed towards an object outside the body. The other is the "thermal comfort/discomfort" of the body that is important for thermoregulation. We recently reported rCBF changes in the amygdala which correlated with thermal comfort during whole body cooling (Kanosue et al., 2000). In the present study we investigated the region of brain that is activated by local thermal stimulation of the hand. Eight healthy subjects were recruited and gave written informed consent to participate in the study. Warm (39 degrees C) or cold (25 degrees C) stimuli was applied to the right or left hand for 30 s by using a water circulating tube that covered the whole hand. Each subject reported the magnitude of the stimulus intensity of temperature sensation using a scale from 1 (very cold) to 9 (very hot). All subjects reported hot or cold sensations and not pain. We examined the correlation between the rating scores and regional activity over the entire brain with a 3 Tesla MR imagers (VP, General Electrics, Milwaukee, US). Activation was observed in the contralateral secondary somatosensory cortex in response to both warm and cold stimulation of the hand. No activation was observed in the amygdala. This suggests that temperature sensation and thermal comfort might be generated by completely different structures of the brain.

    DOI

  • The involvement of Cry1 and Cry2 genes in the regulation of the circadian body temperature rhythm in mice.

    Kei Nagashima, Kenta Matsue, Masahiro Konishi, Chisato Iidaka, Koyomi Miyazaki, Norio Ishida, Kazuyuki Kanosue

    American journal of physiology. Regulatory, integrative and comparative physiology   288 ( 1 ) R329-35 - R335  2005.01  [Refereed]  [International journal]

     View Summary

    The criptochrome genes (Cry1 and Cry2) are involved in the molecular mechanism that controls the circadian clock, and mice lacking these genes (Cry1(-/-)/Cry2(-/-)) are behaviorally arrhythmic. It has been speculated that the circadian clock modulates the characteristics of thermoregulation, resulting in body temperature (T(b)) rhythm. However, there is no direct evidence proving this speculation. We show here that T(b) and heat production in Cry1(-/-)/Cry2(-/-) mice are arrhythmic under constant darkness. In contrast, both rhythms occur under a light-dark cycle and/or periodical food restriction linked with spontaneous activity and/or eating, although they are not robust as those in wild-type mice. The relationship between heat production and T(b) in Cry1(-/-)/Cry2(-/-) mice is linear and identical under any conditions, indicating that their T(b) rhythm is determined by heat production rhythm associated with activity and eating. However, T(b) in wild-type mice is maintained at a relatively higher level in the active phase than the inactive phase regardless of the heat production level. These results indicate that the thermoregulatory responses are modulated according to the circadian phase, and the Cry genes are involved in this mechanism.

    DOI PubMed

  • Fos activation in hypothalamic neurons during cold or warm exposure: Projections to periaqueductal gray matter

    K Yoshida, M Konishi, K Nagashima, CB Saper, K Kanosue

    NEUROSCIENCE   133 ( 4 ) 1039 - 1046  2005  [Refereed]

     View Summary

    The hypothalamus, especially the preoptic area, plays a crucial role in thermoregulation, and our previous studies showed that the periaqueductal gray matter is important for transmitting efferent signals to thermoregulatory effectors in rats. Neurons responsible for skin vasodilation are located in the lateral portion of the rostral periaqueductal gray matter, and neurons that mediate non-shivering thermogenesis are located in the ventrolateral part of the caudal periaqueductal gray matter. We investigated the distribution of neurons in the rat hypothalamus that are activated by exposure to neutral (26 degrees C), warm (33 degrees C), or cold (10 degrees C) ambient temperature and project to the rostral periaqueductal gray matter or caudal periaqueductal gray matter, by using the immunohistochemical analysis of Fos and a retrograde tracer, cholera toxin-b. When cholera toxin-b was injected into the rostral periaqueductal gray matter, many double-labeled cells were observed in the median preoptic nucleus in warm-exposed rats, but few were seen in cold-exposed rats. On the other hand, when cholera toxin-b was injected into the caudal periaqueductal gray matter, many double-labeled cells were seen in a cell group extending from the dorsomedial nucleus through the dorsal hypothalamic area in cold-exposed rats but few were seen in warm-exposed rats. These results suggest that the rostral periaqueductal gray matter receives input from the median preoptic nucleus neurons activated by warm exposure, and the caudal periaqueductal gray matter receives input from neurons in the dorsomedial nucleus/dorsal hypothalamic area region activated by cold exposure. These efferent pathways provide a substrate for thermoregulatory skin vasomotor response and non-shivering thermogenes is, respectively. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.

    DOI

  • 体温調節のメカニズム(共著)

    彼末一之, 永島 計, 小西あき

    体育の科学   54 ( 10 ) 764 - 768  2004.10

  • Brain regions expressing Fos during thermoregulatory behavior in rats.

    Megumi Maruyama, Maiko Nishi, Masahiro Konishi, Yuko Takashige, Kei Nagashima, Toshikazu Kiyohara, Kazuyuki Kanosue

    American journal of physiology. Regulatory, integrative and comparative physiology   285 ( 5 ) R1116-23 - R1123  2003.11  [Refereed]  [International journal]

     View Summary

    We surveyed the neural substrata for behavioral thermoregulation with immunohistochemical analysis of the expression of Fos protein in the rat brain. We used an operant system in which a rat exposed to heat (40 degrees C) could get cold air (0 degrees C) for 30 s when it moved into the reward area. Rats moved in and out of the reward area of the system periodically and thus maintained their body temperature at a normal level. In the rats performing heat escape behavior (active group), strong Fos immunoreactivity (Fos-IR) was found in the median preoptic nucleus (MnPO), parastrial nucleus (PS), and dorsomedial hypothalamus (DMH) compared with the controls. Another group of rats (passive group) were given the same temperature changes, regardless of the rat's movement, as those obtained by rats of the active group. Fos-IR in the MnPO was also seen in this group. The present results suggest that the PS and DMH play an important role in the genesis of thermoregulatory behavior, whereas the MnPO may be important for detecting changes in ambient and/or body temperatures.

    DOI PubMed

  • Brain regions expressing Fos during thermoregulatory behavior in rats

    Maruyama, M, Nishi, M, Konishi, M, Takashige, Y, Nagashima, K, Kiyohara, T, Kanosue, K

    American Journal of Physiology - Regulatory Integrative and Comparative Physiology   285 ( 5 54-5 ) R1116 - R1123  2003.09  [Refereed]

    DOI PubMed

  • Attenuation of metabolic heat production and cold-escape/warm-seeking behaviour during a cold exposure following systemic salt loading in rats.

    Masahiro Konishi, Kei Nagashima, Kento Asano, Kazuyuki Kanosue

    The Journal of physiology   551 ( Pt 2 ) 713 - 20  2003.09  [Refereed]  [International journal]

     View Summary

    The reduction of body core temperature (Tcore) after salt loading has been reported. In this study, we tested the hypothesis that, during a cold exposure in rats, (1) salt loading would decrease metabolic rate (MR), reducing Tcore, but (2) Tcore would be maintained when cold-escape/warm-seeking behaviour is available. In the first experiment (n = 7), MR and Tcore were measured by indirect calorimetry and telemetry, respectively, during 26, 20 and 10 degrees C exposure for 1 h each, in that order. In the second experiment (n = 7), each rat was placed in an operant system during the same exposure protocol as in the first experiment, where it could trigger a 40 degrees C air reward for 30 s at 20 and 10 degrees C by moving into specific areas (operant behaviour). In each experiment, rats repeated the same protocol twice with a subcutaneous injection (10 ml kg-1) of either isotonic saline (154 mM) or hypertonic saline (2500 mM). In the first experiment, MR in the isotonic-saline trial increased (P < 0.05) at 20 and 10 degrees C compared with that at 26 degrees C by 21 +/- 5 and 48 +/- 6 %, respectively (means +/- S.E.M.), with Tcore unchanged. However, values for MR and Tcore in the hypertonic-saline trial were lower (P < 0.05) than those in the isotonic-saline trial in any ambient temperature. In the second experiment, Tcore was also lower (P < 0.05) in the hypertonic-saline trial than in the isotonic-saline trial. The counts of the operant behaviour in the hypertonic-saline trial remained unchanged in each exposure period, but those in the isotonic-saline trial increased (P < 0.05) at 10 degrees C. These results may suggest that salt loading attenuates both metabolic and behavioural thermoregulatory responses to the cold.

    DOI PubMed

  • Effects of fasting on thermoregulatory processes and the daily oscillations in rats.

    Kei Nagashima, Sadamu Nakai, Kenta Matsue, Masahiro Konishi, Mutsumi Tanaka, Kazuyuki Kanosue

    American journal of physiology. Regulatory, integrative and comparative physiology   284 ( 6 ) R1486-93 - R1493  2003.06  [Refereed]  [International journal]

     View Summary

    To investigate the mechanism involved in the reduction of body core temperature (T(core)) during fasting in rats, which is selective in the light phase, we measured T(core), surface temperature, and oxygen consumption rate in fed control animals and in fasted animals on day 3 of fasting and day 4 of recovery at an ambient temperature (T(a)) of 23 degrees C by biotelemetry, infrared thermography, and indirect calorimetry, respectively. On the fasting day, 1) T(core) in the light phase decreased (P < 0.05) from the control; however, T(core) in the dark phase was unchanged, 2) tail temperature fell from the control (P < 0.05, from 30.7 +/- 0.1 to 23.9 +/- 0.1 degrees C in the dark phase and from 29.4 +/- 0.1 to 25.2 +/- 0.2 degrees C in the light phase), 3) oxygen consumption rate decreased from the control (P < 0.05, from 24.37 +/- 1.06 to 16.24 +/- 0.69 ml. min(-1). kg body wt(-0.75) in the dark phase and from 18.91 +/- 0.64 to 14.00 +/- 0.41 ml. min(-1). kg body wt(-0.75) in the light phase). All these values returned to the control levels on the recovery day. The results suggest that, in the fasting condition, T(core) in the dark phase was maintained by suppression of the heat loss mechanism, despite the reduction of metabolic heat production. In contrast, the response was weakened in the light phase, decreasing T(core) greatly. Moreover, the change in the regulation of tail blood flow was a likely mechanism to suppress heat loss.

    DOI PubMed

  • Effects of fasting on thermoregulatory processes and the daily oscillations in rats

    K Nagashima, S Nakai, K Matsue, M Konishi, M Tanaka, K Kanosue

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   284 ( 6 ) R1486 - R1493  2003.06

     View Summary

    To investigate the mechanism involved in the reduction of body core temperature (T-core) during fasting in rats, which is selective in the light phase, we measured T-core, surface temperature, and oxygen consumption rate in fed control animals and in fasted animals on day 3 of fasting and day 4 of recovery at an ambient temperature (T-a) of 23 degreesC by biotelemetry, infrared thermography, and indirect calorimetry, respectively. On the fasting day, 1) T-core in the light phase decreased (P &lt; 0.05) from the control; however, T-core in the dark phase was unchanged, 2) tail temperature fell from the control (P &lt; 0.05, from 30.7 +/- 0.1 to 23.9 +/- 0.1 degreesC in the dark phase and from 29.4 +/- 0.1 to 25.2 +/- 0.2 degreesC in the light phase), 3) oxygen consumption rate decreased from the control ( P &lt; 0.05, from 24.37 +/- 1.06 to 16.24 +/- 0.69 ml . min(-1) . kg body wt(-0.75) in the dark phase and from 18.91 +/- 0.64 to 14.00 +/- 0.41 ml . min(-1) . kg body wt(-0.75) in the light phase). All these values returned to the control levels on the recovery day. The results suggest that, in the fasting condition, T-core in the dark phase was maintained by suppression of the heat loss mechanism, despite the reduction of metabolic heat production. In contrast, the response was weakened in the light phase, decreasing T-core greatly. Moreover, the change in the regulation of tail blood flow was a likely mechanism to suppress heat loss.

    DOI

  • Involvement of the raphé pallidus in the suppressive effect of preoptic warming on non-shivering thermogenesis in rats.

    Aki Taniguchi, Xiao Ming Chen, Kei Nagashima, Mutsumi Tanaka, Kazuyuki Kanosue

    Brain research   966 ( 1 ) 103 - 9  2003.03  [Refereed]  [International journal]

     View Summary

    Thermogenesis in the brown adipose tissue (BAT) is activated by the stimulation of the ventromedial hypothalamic nucleus (VMH). Local warming of the preoptic area (PO) suppresses this response. Injection of the GABA(A) receptor antagonist bicuculline into the caudal periaqueductal gray (cPAG), where excitatory neurons for BAT thermogenesis are located, did not influence the suppressive effect of PO warming. On the other hand, after bicuculline injection into the raphé pallidus, where excitatory neurons for BAT thermogenesis are also located, VMH stimulation produced BAT thermogenesis even during PO warming. The present results suggest that the inhibitory signal from the PO reaches the raphé pallidus and not the cPAG for the control of BAT thermogenesis.

    DOI PubMed

  • Effects of fasting on thermoregulatory processes and the daily oscillations in rats

    K Nagashima, S Nakai, K Matsue, K Kanosue

    FASEB JOURNAL   17 ( 4 ) A30 - A30  2003.03  [Refereed]

  • Involvement of the raphe pallidus in the suppressive effect of preoptic warming on non-shivering thermogenesis in rats

    A Taniguchi, XM Chen, K Nagashima, M Tanaka, K Kanosue

    BRAIN RESEARCH   966 ( 1 ) 103 - 109  2003.03  [Refereed]

     View Summary

    Thermogenesis in the brown adipose tissue (BAT) is activated by the stimulation of the ventromedial hypothalamic nucleus (VMH). Local warming of the preoptic area (PO) suppresses this response. Injection of the GABA, receptor antagonist bicuculline into the caudal periaqueductal gray (cPAG), where excitatory neurons for BAT thermogenesis are located, did not influence the suppressive effect of PO warming. On the other hand, after bicuculline injection into the raphe pallidus, where excitatory neurons for BAT thermogenesis are also located, VMH stimulation produced BAT thermogenesis even during PO warming. The present results suggest that the inhibitory signal from the PO reaches the raphe pallidus and not the cPAG for the control of BAT thermogenesis. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI

  • 行動性体温調節

    永島 計

    人間科学研究   11 ( 1 ) 207 - 218  2003

  • Systemic salt loading decreases body temperature and increases heat-escape/cold-seeking behaviour via the central AT1 and V1 receptors in rats.

    Masahiro Konishi, Kei Nagashima, Kazuyuki Kanosue

    The Journal of physiology   545 ( 1 ) 289 - 96  2002.11  [Refereed]  [International journal]

     View Summary

    Salt loading decreases body core temperature (T(core)) at neutral ambient temperature (26 degrees C) and increases heat-escape/cold-seeking behaviour in desalivated rats. In this study, we tested the hypothesis that brain angiotensin II (AII) and arginine vasopressin (AVP) are associated with these responses. Surgically desalivated rats (n = 28) were administered an injection (S.C., 10 ml kg(-1)) of either normal saline (154 mM, NS) or hypertonic saline (2500 mM, HS) following an intracerebroventricular injection (10 microl kg(-1)) of an AII AT(1)-receptor antagonist (candesartan, 5 microg microl(-1)), an AVP V(1)-receptor antagonist ((beta-mercapto-beta, beta-cyclopenta-methylene propionyl(1), O-Me-Tyr(2), Arg(8))-vasopressin, 0.5 microg microl(-1)), or normal saline (154 mM). Each rat was placed in a behaviour box, first at 26 degrees C for 1 h to allow the measurement of baseline T(core) and movement. The ambient temperature was then elevated to 40 degrees C for the next 2 h, during which time the rat was able to trigger a 0 degrees C air reward for 30 s by moving into a specific area of the box (operant behaviour). The S.C. HS significantly decreased baseline T(core) at 26 degrees C (36.5 +/- 0.1 degrees C) and increased counts of operant behaviour at 40 degrees C (57 +/- 3) compared with results obtained following S.C. NS injection (37.4 +/- 0.1 degrees C and 42 +/- 1, respectively). These responses to s.c. HS were inhibited by the intracerebroventricular injection of AT(1) (37.3 +/- 0.1 degrees C and 43 +/- 2, respectively; P < 0.05) and V(1) antagonists (37.2 +/- 0.2 degrees C and 42 +/- 2, respectively; P < 0.05), although administration of both antagonists with S.C. NS had no effect. These results suggest that brain AII and AVP are involved in the decrease in T(core) observed at neutral ambient temperature and the increase in heat-escape/cold-seeking behaviour in response to osmotic stimulation, via the central AT(1) and V(1) receptors, respectively

    DOI PubMed

  • Systemic salt loading decreases body temperature and increases heat-escape/cold-seeking behaviour via the central AT(1) and V-1 receptors in rats

    M Konishi, K Nagashima, K Kanosue

    JOURNAL OF PHYSIOLOGY-LONDON   545 ( 1 ) 289 - 296  2002.11  [Refereed]

     View Summary

    Salt loading decreases body core temperature (T-core) at neutral ambient temperature (26degreesC) and increases heat-escape/cold-seeking behaviour in desalivated rats. In this study, we tested the hypothesis that brain angiotensin II (AII) and arginine vasopressin (AVP) are associated with these responses. Surgically desalivated rats (n = 28) were administered an injection (s.c., 10 ml kg(-1)) of either normal saline (154 mm, NS) or hypertonic saline (2500 mm, HS) following an intracerebroventricular injection (10 mul kg(-1)) of an AII AT(1)-receptor antagonist (candesartan, 5 mug mul(-1)), an AVP V-1-receptor antagonist ((beta-mercapto-beta, beta-cyclopenta-methylene propionyl(1), O-Me-Tyr(2), Arg(8))- vasopressin, 0.5 mug mul(-1)), or normal saline (154 mm). Each rat was placed in a behaviour box, first at 26degreesC for 1 h to allow the measurement of baseline T-core and movement. The ambient temperature was then elevated to 40 degreesC for the next 2 h, during which time the rat was able to trigger a 0 degreesC air reward for 30 s by moving into a specific area of the box (operant behaviour). The s.c. HS significantly decreased baseline T-core at 26 degreesC (36.5 +/- 0.1 degreesC) and increased counts of operant behaviour at 40degreesC (57 +/- 3) compared with results obtained following s.c. NS injection (37.4 +/- 0.1degreesC and 42 +/- 1, respectively). These responses to s.c. HS were inhibited by the intracerebroventricular injection of AT(1) (37.3 +/- 0.1 degreesC and 43 +/- 2, respectively; P &lt; 0.05) and V-1 antagonists (37.2 +/- 0.2&DEG;C and 42 +/- 2, respectively; P &lt; 0.05), although administration of both antagonists with s.c. NS had no effect. These results suggest that brain AII and AVP are involved in the decrease in T-core observed at neutral ambient temperature and the increase in heat-escape/cold-seeking behaviour in response to osmotic stimulation, via the central AT(1) and V-1 receptors, respectively.

    DOI

  • The caudal periaqueductal gray participates in the activation of brown adipose tissue in rats.

    Xiao-Ming Chen, Maiko Nishi, Aki Taniguchi, Kei Nagashima, Masaaki Shibata, Kazuyuki Kanosue

    Neuroscience letters   331 ( 1 ) 17 - 20  2002.10  [Refereed]  [International journal]

     View Summary

    To investigate the involvement of the periaqueductal gray (PAG) in the control of non-shivering thermogenesis, we tested the effects of electrical or chemical stimulation of the PAG on thermogenesis of brown adipose tissue (BAT) in urethane anesthetized rats. Electrical stimulation (0.1 mA, 33 Hz, 0.5 ms) or application of D,L-homocysteic acid (0.5 mM, 0.3 micro l) into the lateral region of the caudal PAG (cPAG) elicited BAT thermogenesis, measured as a rise in the local temperature of interscapular BAT. These results suggest that neurons in the cPAG send excitatory efferent signals for BAT thermogenesis.

    DOI DOI2 PubMed

  • The caudal periaqueductal gray participates in the activation of brown adipose tissue in rats

    XM Chen, M Nishi, A Taniguchi, K Nagashima, M Shibata, K Kanosue

    NEUROSCIENCE LETTERS   331 ( 1 ) 17 - 20  2002.10  [Refereed]

     View Summary

    To investigate the involvement of the periaqueductal gray (PAG) in the control of non-shivering thermogenesis, we tested the effects of electrical or chemical stimulation of the PAG on thermogenesis of brown adipose tissue (BAT) in urethane anesthetized rats. Electrical stimulation (0.1 mA, 33 Hz, 0.5 ms) or application of D,L-homocysteic acid (0.5 mM, 0.3 mul) into the lateral region of the caudal PAG (cPAG) elicited BAT thermogenesis, measured as a rise in the local temperature of interscapular BAT. These results suggest that neurons in the cPAG send excitatory efferent signals for BAT thermogenesis. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

    DOI

  • Brain activation during whole body cooling in humans studied with functional magnetic resonance imaging.

    Kazuyuki Kanosue, Norihiro Sadato, Tomohisa Okada, Tamae Yoda, Sadamu Nakai, Kyoko Yoshida, Takayoshi Hosono, Kei Nagashima, Tomoko Yagishita, Osamu Inoue, Kaoru Kobayashi, Yoshiharu Yonekura

    Neuroscience letters   329 ( 2 ) 157 - 60  2002.08  [Refereed]  [International journal]

     View Summary

    Regional activation of the brain was studied in humans using functional magnetic resonance imaging during whole body cooling that produced thermal comfort/discomfort. Eight normal male subjects lay in a sleeping bag through which air was blown, exposing subjects to cold air (8 degrees C) for 22 min. Each subject scored their degree of thermal comfort and discomfort every min. As the subjects reported more discomfort the blood oxygen level dependent response in the bilateral amygdala increased. There was no activation in the thalamus, somatosensory, cingulate, or insula cortices. This result suggests that the amygdala plays a role in the genesis of thermal discomfort due to cold.

    DOI PubMed

  • Brain activation during whole body cooling in humans studied with functional magnetic resonance imaging

    K Kanosue, N Sadato, T Okada, T Yoda, S Nakai, K Yoshida, T Hosono, K Nagashima, T Yagishita, O Inoue, K Kobayashi, Y Yonekura

    NEUROSCIENCE LETTERS   329 ( 2 ) 157 - 160  2002.08  [Refereed]

     View Summary

    Regional activation of the brain was studied in humans using functional magnetic resonance imaging during whole body cooling that produced thermal comfort/discomfort. Eight normal male subjects lay in a sleeping bag through which air was blown, exposing subjects to cold air (8degreesC) for 22 min. Each subject scored their degree of thermal comfort and discomfort every min. As the subjects reported more discomfort the blood oxygen level dependent response in the bilateral amygdala increased. There was no activation in the thalamus, somatosensory, cingulate, or insula cortices. This result suggests that the amygdala plays a role in the genesis of thermal discomfort due to cold. (C) 2002 Published by Elsevier Science Ireland Ltd.

    DOI

  • Fos expression induced by warming the preoptic area in rats.

    Kyoko Yoshida, Megumi Maruyama, Takayoshi Hosono, Kei Nagashima, Yutaka Fukuda, Ruediger Gerstberger, Kazuyuki Kanosue

    Brain research   933 ( 2 ) 109 - 17  2002.04  [Refereed]  [International journal]

     View Summary

    The preoptic area (POA) occupies a crucial position among the structures participating in thermoregulation, but we know little about its efferent projections for controlling various effector responses. In this study, we used an immunohistochemical analysis of Fos expression during local warming of the preoptic area. To avoid the effects of anesthesia or stress, which are known to elicit Fos induction in various brain regions, we used a novel thermode specifically designed for chronic warming of discrete brain structures in freely moving rats. At an ambient temperature of 22 degrees C, local POA warming increased Fos immunoreactivity in the supraoptic nucleus (SON) and the periaqueductal gray matter (PAG). Exposure of animals to an ambient temperature of 5 degrees C induced Fos immunoreactivity in the magnocellular paraventricular nucleus (mPVN) and the dorsomedial region of the hypothalamus (DMH). Concurrent warming of the POA suppressed Fos expression in these areas. These findings suggest that thermal information from the preoptic area sends excitatory signals to the SON and the PAG, and inhibitory signals to the mPVN and the DMH.

    DOI DOI2 PubMed

  • Role of the medullary raphé in thermoregulatory vasomotor control in rats.

    Mutsumi Tanaka, Kei Nagashima, Robin M McAllen, Kazuyuki Kanosue

    The Journal of physiology   540 ( Pt 2 ) 657 - 64  2002.04  [Refereed]  [International journal]

     View Summary

    To investigate the involvement of the medullary raphé in thermoregulatory vasomotor control, we chemically manipulated raphé neuronal activity while monitoring the tail vasomotor response to preoptic warming. For comparison, neuronal activity in the rostral ventrolateral medulla (RVLM) was manipulated in similar experiments. Injections of D,L-homocysteic acid (DLH; 0.5 mM, 0.3 microl) into a restricted region of the ventral medullary raphé suppressed the tail vasodilatation normally elicited by warming the preoptic area to 42 degrees C. DLH injection into the RVLM also suppressed the vasodilatation elicited by preoptic warming. Injection of bicuculline (0.5 mM, 0.3 microl) into the same raphé region suppressed the vasodilatation elicited by preoptic warming. Bicuculline injection into the RVLM did not suppress tail vasodilatation. These results suggest that neurones in both the medullary raphé and the RVLM are vasoconstrictor to the tail, but only those in the raphé receive inhibitory input from the preoptic area. That input might be direct and/or indirect (e.g. via the periaqueductal grey matter).

    DOI PubMed

  • Role of the medullary raphe in thermoregulatory vasomotor control in rats

    M Tanaka, K Nagashima, RM McAllen, K Kanosue

    JOURNAL OF PHYSIOLOGY-LONDON   540 ( 2 ) 657 - 664  2002.04  [Refereed]

     View Summary

    To investigate the involvement of the medullary raphe in thermoregulatory vasomotor control, we chemically manipulated raphe neuronal activity while monitoring the tail vasomotor response to preoptic warming. For comparison, neuronal activity in the rostral ventrolateral medulla (RVLM) was manipulated in similar experiments. Injections Of D,L-homocysteic acid (DLH; 0.5 mm, 0.3 mul) into a restricted region of the ventral medullary raphe suppressed the tail vasodilatation normally elicited by warming the preoptic area to 42degreesC. DLH injection into the RVLM also suppressed the vasodilatation elicited by preoptic warming. Injection of bicuculline (0.5 mm, 0.3 mul) into the same raphe region suppressed the vasodilatation elicited by preoptic warming. Bicuculline injection into the RVLM did not suppress tail vasodilatation. These results suggest that neurones in both the medullary raphe and the RVLM are vasoconstrictor to the tail, but only those in the raphe receive inhibitory input from the preoptic area. That input might be direct and/or indirect (e.g. via the periaqueductal grey matter).

    DOI

  • Fos expression induced by warming the preoptic area in rats

    K Yoshida, M Maruyama, T Hosono, K Nagashima, Y Fukuda, R Gerstberger, K Kanosue

    BRAIN RESEARCH   933 ( 2 ) 109 - 117  2002.04  [Refereed]

     View Summary

    The preoptic area (POA) occupies a crucial position among the structures participating in thermoregulation, but we know little about its efferent projections for controlling various effector responses. In this study, we used an immunohistochemical analysis of Fos expression during local warming of the preoptic area. To avoid the effects of anesthesia or stress, which are known to elicit Fos induction in various brain regions, we used a novel thermode specifically designed for chronic warming of discrete brain structures in freely moving rats. At an ambient temperature of 22 degreesC, local POA warming increased Fos immunoreactivity in the supraoptic nucleus (SON) and the periaqueductal gray matter (PAG). Exposure of animals to an ambient temperature of 5 degreesC induced Fos immunoreactivity in the magnocellular paraventricular nucleus (mPVN) and the dorsomedial region of the hypothalamus (DMH). Concurrent warming of the POA suppressed Fos expression in these areas. These findings suggest that thermal information from the preoptic area sends excitatory signals to the SON and the PAG, and inhibitory signals to the mPVN and the DMH. (C) 2002 Elsevier Science BV All rights reserved.

    DOI

  • Thermal regulation and comfort during a mild-cold exposure in young Japanese women complaining of unusual coldness.

    Kei Nagashima, Tamae Yoda, Tomoko Yagishita, Aki Taniguchi, Takayoshi Hosono, Kazuyuki Kanosue

    Journal of applied physiology (Bethesda, Md. : 1985)   92 ( 3 ) 1029 - 35  2002.03  [Refereed]  [International journal]

     View Summary

    We examined body core and skin temperatures and thermal comfort in young Japanese women suffering from unusual coldness (C, n = 6). They were selected by interview asking whether they often felt severe coldness even in an air-conditioned environment (20-26 degrees C) and compared with women not suffering from coldness (N, n = 6). Experiments were conducted twice for each subject: 120-min exposure at 23.5 degrees C or 29.5 degrees C after a 40-min baseline at 29.5 degrees C. Mean skin temperature decreased (P < 0.05) from 33.6 +/- 0.1 degrees C (mean +/- SE) to 31.1 +/- 0.1 degrees C and from 33.5 +/- 0.1 degrees C to 31.1 +/- 0.1 degrees C in C and N during the 23.5 degrees C exposure. Fingertip temperature in C decreased more than in N (P < 0.05; from 35.2 +/- 0.1 degrees C to 23.6 +/- 0.2 degrees C and from 35.5 +/- 0.1 degrees C to 25.6 +/- 0.6 degrees C). Those temperatures during the 29.5 degrees C exposure remained at the baseline levels. Rectal temperature during the 23.5 degrees C exposure was maintained at the baseline level in both groups (from 36.9 +/- 0.2 degrees C to 36.8 +/- 0.1 degrees C and 37.1 +/- 0.1 degrees C to 37.0 +/- 0.1 degrees C in C and N). The rating scores of cold discomfort for both the body and extremities were greater (P < 0.05) in C than in N. Thus the augmented thermal sensitivity of the body to cold and activated vasoconstriction of the extremities during cold exposure could be the mechanism for the severe coldness felt in C.

    DOI PubMed

  • Involvement of the suprachiasmatic nucleus in body temperature modulation by food deprivation in rats.

    Su Liu, Xiao Ming Chen, Tamae Yoda, Kei Nagashima, Yutaka Fukuda, Kazuyuki Kanosue

    Brain research   929 ( 1 ) 26 - 36  2002.03  [Refereed]  [International journal]

     View Summary

    Recently we found that food-deprived rats kept under a light-dark cycle showed a progressive reduction in body temperature during the light phase on each subsequent day while body temperature in the dark phase did not differ from baseline values. In this study, we investigated the effect of lesioning the hypothalamic suprachiasmatic nucleus (SCN) on body temperature modulation by food deprivation. In the SCN-lesioned rats in which daily rhythms of body temperature and activity were abolished, body temperature was unchanged by food deprivation. We also examined the effect of food deprivation on the daily changes in Fos expression in the SCN. Under normal fed conditions the number of SCN cells expressing Fos is high during the day and low at night. Food deprivation attenuated the amplitude of this daily change in Fos expression in the SCN. This tendency was prominent in the dorsal part of the SCN, while the ventral part showed no effect of food deprivation. These findings suggest that the SCN plays some role in body temperature modulation due to food deprivation.

    DOI DOI2 PubMed

  • Thermal regulation and comfort during a mild-cold exposure in young Japanese women complaining of unusual coldness

    K Nagashima, T Yoda, T Yagishita, A Taniguchi, T Hosono, K Kanosue

    JOURNAL OF APPLIED PHYSIOLOGY   92 ( 3 ) 1029 - 1035  2002.03

     View Summary

    We examined body core and skin temperatures and thermal comfort in young Japanese women suffering from unusual coldness (C, n=6). They were selected by interview asking whether they often felt severe coldness even in an air-conditioned environment (20-26degreesC) and compared with women not suffering from coldness (N, n=6). Experiments were conducted twice for each subject: 120-min exposure at 23.5degreesC or 29.5degreesC after a 40-min baseline at 29.5degreesC. Mean skin temperature decreased (P&lt;0.05) from 33.6&PLUSMN;0.1&DEG;C (mean &PLUSMN; SE) to 31.1&PLUSMN;0.1&DEG;C and from 33.5&PLUSMN;0.1&DEG;C to 31.1&PLUSMN;0.1&DEG;C in C and N during the 23.5&DEG;C exposure. Fingertip temperature in C decreased more than in N (P&lt;0.05; from 35.2+/-0.1degreesC to 23.6+/-0.2degreesC and from 35.5+/-0.1degreesC to 25.6+/-0.6degreesC). Those temperatures during the 29.5degreesC exposure remained at the baseline levels. Rectal temperature during the 23.5degreesC exposure was maintained at the baseline level in both groups (from 36.9+/-0.2degreesC to 36.8+/-0.1degreesC and 37.1+/-0.1degreesC to 37.0+/-0.1degreesC in C and N). The rating scores of cold discomfort for both the body and extremities were greater (P&lt;0.05) in C than in N. Thus the augmented thermal sensitivity of the body to cold and activated vasoconstriction of the extremities during cold exposure could be the mechanism for the severe coldness felt in C.

    DOI

  • Involvement of the suprachiasmatic nucleus in body temperature modulation by food deprivation in rats

    S Liu, XM Chen, T Yoda, K Nagashima, Y Fukuda, K Kanosue

    BRAIN RESEARCH   929 ( 1 ) 26 - 36  2002.03  [Refereed]

     View Summary

    Recently we found that food-deprived rats kept under a light-dark cycle showed a progressive reduction in body temperature during the light phase on each subsequent day while body temperature in the dark phase did not differ from baseline values. In this study, we investigated the effect of lesioning the hypothalamic suprachiasmatic nucleus (SCN) on body temperature modulation by food deprivation. In the SCN-lesioned rats in which daily rhythms of body temperature and activity were abolished, body temperature was unchanged by food deprivation. We also examined the effect of food deprivation on the daily changes in Fos expression in the SCN. Under normal fed conditions the number of SCN cells expressing Fos is high during the day and low at night. Food deprivation attenuated the amplitude of this daily change in Fos expression in the SCN. This tendency was prominent in the dorsal part of the SCN. while the ventral part showed no effect of food deprivation. These findings suggest that the SCN plays some role in body temperature modulation due to food deprivation. (C) 2002 Elsevier Science B.V. All rights reserved.

    DOI

  • 温熱的快・不快感(暑さ・寒さ)とは(共著)

    彼末 一之, 永島 計, 谷口 あき, 小西 正博

    繊維製品消費科学   43 ( 3 ) 17 - 22  2002

    DOI

  • Hypothalamic region facilitating shivering in rats

    M Tanaka, M Tonouchi, T Hosono, K Nagashima, M Yanase-Fujiwara, K Kanosue

    JAPANESE JOURNAL OF PHYSIOLOGY   51 ( 5 ) 625 - 629  2001.10  [Refereed]

     View Summary

    Although the posterior part of the hypothalamus has long, been considered important for thermoregulatory shivering, it is unknown whether the neurons there or the passing fibers are implicated in the response. Exposure of urethane-anesthetized rats to cold (15-21 degreesC) elicits shivering. An injection of muscimol (0.5 mm), a GABA(A) receptor agonist, into the medial part of the hypothalamus, including the dorsomedial and posterior nuclei, suppressed the cold-induced shivering. This result suggests that neurons having an excitatory effect on shivering are in this region of the hypothalamus.

    DOI

  • Increased renal tubular sodium reabsorption during exercise-induced hypervolemia in humans

    K Nagashima, JC Wu, SA Kavouras, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   91 ( 3 ) 1229 - 1236  2001.09  [Refereed]

     View Summary

    We tested the hypothesis that renal tubular Na+ reabsorption increased during the first 24 h of exercise-induced plasma volume expansion. Renal function was assessed 1 day after no-exercise control (C) or intermittent cycle ergometer exercise (Ex, 85% of peak O-2 uptake) for 2 h before and 3 h after saline loading (12.5 ml/kg over 30 min) in seven subjects. Ex reduced renal blood flow (p-aminohippurate clearance) compared with C (0.83 +/- 0.12 vs. 1.49 +/- 0.24 l/min, P&lt; 0.05) but did not influence glomerular filtration rates (97 +/- 10 ml/min, inulin clearance). Fractional tubular reabsorption of Na+ in the proximal tubules was higher in Ex than in C (P&lt; 0.05). Saline loading decreased fractional tubular reabsorption of Na+ from 99.1 +/- 0.1 to 98.7 +/- 0.1% (P&lt; 0.05) in C but not in Ex (99.3 +/- 0.1 to 99.4 +/- 0.1%). Saline loading reduced plasma renin activity and plasma arginine vasopressin levels in C and Ex, although the magnitude of decrease was greater in C (P&lt; 0.05). These results indicate that, during the acute phase of exercise-induced plasma volume expansion, increased tubular Na+ reabsorption is directed primarily to the proximal tubules and is associated with a decrease in renal blood flow. In addition, saline infusion caused a smaller reduction in fluid-regulating hormones in Ex. The attenuated volume-regulatory response acts to preserve distal tubular Na+ reabsorption during saline infusion 24 h after exercise.

  • Increased renal tubular sodium reabsorption during exercise-induced hypervolemia in humans

    K Nagashima, JC Wu, SA Kavouras, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   91 ( 3 ) 1229 - 1236  2001.09

     View Summary

    We tested the hypothesis that renal tubular Na+ reabsorption increased during the first 24 h of exercise-induced plasma volume expansion. Renal function was assessed 1 day after no-exercise control (C) or intermittent cycle ergometer exercise (Ex, 85% of peak O-2 uptake) for 2 h before and 3 h after saline loading (12.5 ml/kg over 30 min) in seven subjects. Ex reduced renal blood flow (p-aminohippurate clearance) compared with C (0.83 +/- 0.12 vs. 1.49 +/- 0.24 l/min, P&lt; 0.05) but did not influence glomerular filtration rates (97 +/- 10 ml/min, inulin clearance). Fractional tubular reabsorption of Na+ in the proximal tubules was higher in Ex than in C (P&lt; 0.05). Saline loading decreased fractional tubular reabsorption of Na+ from 99.1 +/- 0.1 to 98.7 +/- 0.1% (P&lt; 0.05) in C but not in Ex (99.3 +/- 0.1 to 99.4 +/- 0.1%). Saline loading reduced plasma renin activity and plasma arginine vasopressin levels in C and Ex, although the magnitude of decrease was greater in C (P&lt; 0.05). These results indicate that, during the acute phase of exercise-induced plasma volume expansion, increased tubular Na+ reabsorption is directed primarily to the proximal tubules and is associated with a decrease in renal blood flow. In addition, saline infusion caused a smaller reduction in fluid-regulating hormones in Ex. The attenuated volume-regulatory response acts to preserve distal tubular Na+ reabsorption during saline infusion 24 h after exercise.

  • Increased heat-escape/cold-seeking behavior following hypertonic saline injection in rats

    K Nagashima, S Nakai, M Konishi, L Su, K Kanosue

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   280 ( 4 ) R1031 - R1036  2001.04  [Refereed]

     View Summary

    We examined the effect of hypertonic saline injection on heat-escape/cold-seeking behavior in desalivated rats. Rats were exposed to 40 degreesC heat after normal (154 mM NaCl, control) or hypertonic saline (2,500 mM NaCl) injection (1 ml/100 g body wt). The rats received a 0 degreesC air for 30 s when they entered a specific area in an experimental box. Core temperature (T(c)) surpassed 40 degreesC in both conditions when 0 degreesC air was not available. Hypertonic saline injection produced a lower baseline T(c) than control [36.9 +/- 0.2 and 37.9 +/- 0.2 degreesC (means +/- SE), P &lt; 0.05] and a greater number of 0&lt;degrees&gt;C air rewards during the 2-h heat with lower T(c) at the end (48 +/- 1 and 34 +/- 2, 37.6 +/- 0.1, and 37.3 +/- 0.1 degreesC in the control and hypertonic saline injection trial, respectively, P &lt; 0.05, n = 6). However, T(c) was similar (37.7 +/- 0.2 and 37.6 +/- 0.4&lt;degrees&gt;C in the control and hypertonic saline injection trial, n = 5) when 0 degreesC air was automatically and intermittently (35 times) given during the heat. Rats augment heat-defense mechanisms in response to osmotic stress by lowering the baseline T(c) and increasing heat-escape/cold-seeking behavior.

  • Increased heat-escape/cold-seeking behavior following hypertonic saline injection in rats

    K Nagashima, S Nakai, M Konishi, L Su, K Kanosue

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   280 ( 4 ) R1031 - R1036  2001.04

     View Summary

    We examined the effect of hypertonic saline injection on heat-escape/cold-seeking behavior in desalivated rats. Rats were exposed to 40 degreesC heat after normal (154 mM NaCl, control) or hypertonic saline (2,500 mM NaCl) injection (1 ml/100 g body wt). The rats received a 0 degreesC air for 30 s when they entered a specific area in an experimental box. Core temperature (T(c)) surpassed 40 degreesC in both conditions when 0 degreesC air was not available. Hypertonic saline injection produced a lower baseline T(c) than control [36.9 +/- 0.2 and 37.9 +/- 0.2 degreesC (means +/- SE), P &lt; 0.05] and a greater number of 0&lt;degrees&gt;C air rewards during the 2-h heat with lower T(c) at the end (48 +/- 1 and 34 +/- 2, 37.6 +/- 0.1, and 37.3 +/- 0.1 degreesC in the control and hypertonic saline injection trial, respectively, P &lt; 0.05, n = 6). However, T(c) was similar (37.7 +/- 0.2 and 37.6 +/- 0.4&lt;degrees&gt;C in the control and hypertonic saline injection trial, n = 5) when 0 degreesC air was automatically and intermittently (35 times) given during the heat. Rats augment heat-defense mechanisms in response to osmotic stress by lowering the baseline T(c) and increasing heat-escape/cold-seeking behavior.

  • The Central Organization of the Thermoregulatory System

    Kazuyuki Kanosue, Kyoko Yoshida, Megumi Maruyama, Kei Nagashima

    Thermotherapy for Neoplasia, Inflammation, and Pain     2  2001  [Refereed]

    DOI

  • 視床下部と体温調節(共著)

    Clinical Neuroscience   19   47 - 49  2001

  • 温熱的快・不快感(暑さ・寒さ)の脳内機序(共著)

    KANOSUE K., NAGASHIMA K., YAGISHITA T., TANIGUCHI A., SADATO N., OKADA T., YONIKURA Y.

    Biomedical Thermology   21 ( 2 ) 38 - 44  2001

    CiNii

  • Neuronal circuitries involved in thermoregulation

    K Nagashima, S Nakai, M Tanaka, K Kanosue

    AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL   85 ( 1-3 ) 18 - 25  2000.12  [Refereed]

     View Summary

    The body temperature of homeothermic animals is regulated by systems that utilize multiple behavioral and autonomic effector responses. In the last few years, new approaches have brought us new information and new ideas about neuronal interconnections in the thermoregulatory network. Studies utilizing chemical stimulation of the preoptic area revealed both heat loss and production responses are controlled by warm-sensitive neurons. These neurons send excitatory efferent signals for the heat loss and inhibitory efferent signals for the heat production. The warm-sensitive neurons are separated and work independently to control these two opposing responses. Recent electrophysiological analysis have identified some neurons sending axons directly to the spinal cord for thermoregulatory effector control. Included are midbrain reticulospinal neurons for shivering and premotor neurons in the medulla oblongata for skin vasomotor control. As for the afferent side of the thermoregulatory network, the vagus nerve is recently paid much attention, which would convey signals for peripheral infection to the brain and be responsible for the induction of fever. The vagus nerve may also participate in thermoregulation in afebrile conditions, because some substances such as cholecyctokinin and leptin activate the vagus nerve. Although the functional role for this response is still obscure, the vagus may transfer nutritional and/or metabolic signals to the brain, affecting metabolism and body temperature. (C) 2000 Elsevier Science B.V. All rights reserved.

    DOI DOI2

  • Intense exercise stimulates albumin synthesis in the upright posture

    K Nagashima, GW Cline, GW Mack, GI Shulman, ER Nadel

    JOURNAL OF APPLIED PHYSIOLOGY   88 ( 1 ) 41 - 46  2000.01  [Refereed]

     View Summary

    We tested the hypothesis that an elevation in albumin synthetic rate contributes to increased plasma albumin content during exercise-induced hypervolemia. Albumin synthetic rate was measured in seven healthy subjects at 1-5 and 21-22 h after 72 min of intense (85% peak oxygen consumption rate) intermittent exercise and after 5 h recovery in either upright (Up) or supine (Sup) postures. Deuterated phenylalanine (d(5)-Phe) was administrated by a primed-constant infusion method, and fractional synthetic rate (FSR) and absolute synthetic rate (ASR) of albumin were calculated from the enrichment of dB-Phe in plasma albumin, determined by gas chromatography-mass spectrometry. FSR of albumin in Up increased significantly (P &lt; 0.05) from 4.9 +/- 0.9%/day at control to 7.3 +/- 0.9%/day at 22 h of recovery. ASR of albumin increased from 87.9 +/- 17.0 to 141.1 +/- 16.6 mg albumin . kg body wt(-1) . day(-1). In contrast, FSR and ASR of albumin were unchanged in Sup (3.9 +/- 0.4 to 4.0 +/- 1.4%/day and 74.2 +/- 8.9 to 85.3 +/- 23.9 mg albumin . kg body wt(-1) . day(-1) at control and 22 h of recovery, respectively). Increased albumin synthesis after upright intense exercise contributes to the expansion of greater albumin content and its maintenance. We conclude that stimuli related to posture are critical in modulating the drive for albumin synthesis after intense exercise.

  • Human cardiovascular and humoral responses to moderate muscle activation during dynamic exercise

    T Nishiyasu, K Nagashima, ER Nadel, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   88 ( 1 ) 300 - 307  2000.01  [Refereed]

     View Summary

    We examined the hypothesis that activation of the muscle metaboreflex during dynamic exercise would augment influences tending to cause a rise in arginine vasopressin, plasma renin activity, and catecholamines during dynamic exercise in humans. Ten healthy adults performed 30 min of supine cycle ergometer exercise at similar to 50% of peak oxygen consumption with or without moderate muscle metaboreflex activation by application of 35 mmHg lower body positive pressure (LBPP). Application of LBPP during the first 15 or last 15 min of exercise increased mean arterial blood pressure, plasma lactate concentration, and minute ventilation, indicating an activation of the muscle metaboreflex. These changes were rapidly reversed when LBPP was removed. During exercise at this intensity, LBPP augmented the release of arginine vasopressin and catecholamines but not of plasma renin activity. These results suggest that, although in humans hormonal. responses are induced by moderate activation of the muscle metaboreflex during dynamic exercise, the thresholds for these responses may not be uniform among the various glands and hormones.

  • 温熱的情動感覚(暑さ・寒さ)の脳機構(共著)

    生活の場における温度・湿度と人間 講演論文集     33 - 36  2000

  • Neuronal organizations for thermoregulation "jointly worked"

    Autonomic Neuroscience   85   18 - 25  2000

    DOI

  • Intense exercise stimulates albumin synthesis in the upright posture

    K Nagashima, GW Cline, GW Mack, GI Shulman, ER Nadel

    JOURNAL OF APPLIED PHYSIOLOGY   88 ( 1 ) 41 - 46  2000.01

     View Summary

    We tested the hypothesis that an elevation in albumin synthetic rate contributes to increased plasma albumin content during exercise-induced hypervolemia. Albumin synthetic rate was measured in seven healthy subjects at 1-5 and 21-22 h after 72 min of intense (85% peak oxygen consumption rate) intermittent exercise and after 5 h recovery in either upright (Up) or supine (Sup) postures. Deuterated phenylalanine (d(5)-Phe) was administrated by a primed-constant infusion method, and fractional synthetic rate (FSR) and absolute synthetic rate (ASR) of albumin were calculated from the enrichment of dB-Phe in plasma albumin, determined by gas chromatography-mass spectrometry. FSR of albumin in Up increased significantly (P &lt; 0.05) from 4.9 +/- 0.9%/day at control to 7.3 +/- 0.9%/day at 22 h of recovery. ASR of albumin increased from 87.9 +/- 17.0 to 141.1 +/- 16.6 mg albumin . kg body wt(-1) . day(-1). In contrast, FSR and ASR of albumin were unchanged in Sup (3.9 +/- 0.4 to 4.0 +/- 1.4%/day and 74.2 +/- 8.9 to 85.3 +/- 23.9 mg albumin . kg body wt(-1) . day(-1) at control and 22 h of recovery, respectively). Increased albumin synthesis after upright intense exercise contributes to the expansion of greater albumin content and its maintenance. We conclude that stimuli related to posture are critical in modulating the drive for albumin synthesis after intense exercise.

  • Human cardiovascular and humoral responses to moderate muscle activation during dynamic exercise

    T Nishiyasu, K Nagashima, ER Nadel, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   88 ( 1 ) 300 - 307  2000.01

     View Summary

    We examined the hypothesis that activation of the muscle metaboreflex during dynamic exercise would augment influences tending to cause a rise in arginine vasopressin, plasma renin activity, and catecholamines during dynamic exercise in humans. Ten healthy adults performed 30 min of supine cycle ergometer exercise at similar to 50% of peak oxygen consumption with or without moderate muscle metaboreflex activation by application of 35 mmHg lower body positive pressure (LBPP). Application of LBPP during the first 15 or last 15 min of exercise increased mean arterial blood pressure, plasma lactate concentration, and minute ventilation, indicating an activation of the muscle metaboreflex. These changes were rapidly reversed when LBPP was removed. During exercise at this intensity, LBPP augmented the release of arginine vasopressin and catecholamines but not of plasma renin activity. These results suggest that, although in humans hormonal. responses are induced by moderate activation of the muscle metaboreflex during dynamic exercise, the thresholds for these responses may not be uniform among the various glands and hormones.

  • Neuronal organizations for thermoregulation "jointly worked"

    Nagashima, K, Nakai, S, Tanaka, M, Kanosue, K

    Autonomic Neuroscience: Basic and Clinical   85 ( 1-3 ) 18 - 25  2000  [Refereed]

    DOI

  • CARDIOVASCULAR AND HUMORAL RESPONSES RESULTING FROM MODERATE ACTIVATION OF MUSCLE METABOREFLEX DURING DYNAMIC EXERCISE

    Takeshi Nishiyasu, Kei Nagashima, Ethan R. Nadel, Gary W. Mack

    Medicine & Science in Sports & Exercise   31 ( Supplement ) S226  1999.05  [Refereed]

    DOI

  • Attenuation of urinary sodium excretion during cold-air exposure in trained athletes

    T Yoshida, K Nagashima, S Nakai, A Yorimoto, T Kawabata, T Morimoto

    JAPANESE JOURNAL OF PHYSIOLOGY   49 ( 2 ) 193 - 199  1999.04  [Refereed]

     View Summary

    To clarify whether an increase in urinary sodium (Na) excretion during cold-air exposure is attenuated in trained athletes, we analyzed the urinary and hormonal responses to cold-air exposure at 15 degrees C in trained (TR, n=9) and untrained men (UT, n=9), During 15 degrees C exposure in the UT group, the urinary Na excretion (UNaV), fractional excretion of Na and urinary Na to K ratio (U-Na/K) increased significantly (p&lt;0.01-0.05), but there were no variations in creatinine clearance or the filtered Na load. In the TR group, however, no significant changes were demonstrated in these parameters. The amount of increase in plasma noradrenaline and of decrease in plasma volume were greater, and the ADH and adrenaline responses were smaller in the TR group than those found in the UT group during 15 degrees C air exposure. A significant positive correlation was demonstrated between the increase of UNaV and both the relative changes of U-Na/K and mean blood pressure. These results indicated that natriuresis during cold-air exposure was induced by the decrease in tubular reabsorption of Na, and that natriuresis was attenuated in trained athletes.

    DOI

  • Mechanism for the posture-specific plasma volume increase after a single intense exercise protocol

    K Nagashima, GW Mack, A Haskell, T Nishiyasu, ER Nadel

    JOURNAL OF APPLIED PHYSIOLOGY   86 ( 3 ) 867 - 873  1999.03  [Refereed]

     View Summary

    To test the hypothesis that exercise-induced hypervolemia is a posture-dependent process, we measured plasma volume, plasma albumin content, and renal function in seven healthy subjects for 22 h after single upright (Up) or supine (Sup) intense (85% peak oxygen consumption rate) exercise. This posture was maintained for 5 h after exercise. Plasma volume decreased during exercise but returned to control levels by 5 h of recovery in both postures. By 22 h of recovery, plasma volume increased 2.4 +/- 0.8 ml/kg in Up but decreased 2.1 +/- 0.8 ml/kg in Sup. The plasma volume expansion in Up was accompanied by an increase in plasma albumin content (0.11 +/- 0.04 g/kg; P &lt; 0.05). Plasma albumin content was unchanged in Sup. Urine volume and sodium clearance were lower in Up than Sup (P &lt; 0.05) by 5 h of recovery. These data suggest that increased plasma albumin content contributes to the acute phase of exercise-induced hypervolemia. More importantly, the mechanism by which exercise influences the distribution of albumin between extra- and intravascular stores after exercise is altered by posture and is unknown. We speculate that factors associated with postural changes (e.g., central venous pressure) modify the increase in plasma albumin content and the plasma volume expansion after exercise.

  • Mechanism for the posture-specific plasma volume increase after a single intense exercise protocol

    K Nagashima, GW Mack, A Haskell, T Nishiyasu, ER Nadel

    JOURNAL OF APPLIED PHYSIOLOGY   86 ( 3 ) 867 - 873  1999.03

     View Summary

    To test the hypothesis that exercise-induced hypervolemia is a posture-dependent process, we measured plasma volume, plasma albumin content, and renal function in seven healthy subjects for 22 h after single upright (Up) or supine (Sup) intense (85% peak oxygen consumption rate) exercise. This posture was maintained for 5 h after exercise. Plasma volume decreased during exercise but returned to control levels by 5 h of recovery in both postures. By 22 h of recovery, plasma volume increased 2.4 +/- 0.8 ml/kg in Up but decreased 2.1 +/- 0.8 ml/kg in Sup. The plasma volume expansion in Up was accompanied by an increase in plasma albumin content (0.11 +/- 0.04 g/kg; P &lt; 0.05). Plasma albumin content was unchanged in Sup. Urine volume and sodium clearance were lower in Up than Sup (P &lt; 0.05) by 5 h of recovery. These data suggest that increased plasma albumin content contributes to the acute phase of exercise-induced hypervolemia. More importantly, the mechanism by which exercise influences the distribution of albumin between extra- and intravascular stores after exercise is altered by posture and is unknown. We speculate that factors associated with postural changes (e.g., central venous pressure) modify the increase in plasma albumin content and the plasma volume expansion after exercise.

  • 年齢、体重がエリスロポイエチン(rHuEPO)投与下の造血能に及ぼす影響(共著)

    京都府立医科大学雑誌   108   555 - 564  1999

  • 地球温暖化とヒト

    建築雑誌   114 ( 1445 ) 30 - 33  1999

    CiNii

  • アメニティーを感ずる-温熱的快・不快感の脳内機序-(共著)

    日本熱物性学会創立20周年記念セミナー講演集     11 - 18  1999

  • Erythropoietin投与下の造血能の検討(共著)

    京都府立医科大学雑誌   108   439 - 445  1999

  • 1998 International Symposium on Human Biometeorology

    IPEC     80 - 85  1999

  • Influence of hydrostatic pressure gradients on regulation of plasma volume after exercise

    GW Mack, R Yang, AR Hargens, K Nagashima, A Haskell

    JOURNAL OF APPLIED PHYSIOLOGY   85 ( 2 ) 667 - 675  1998.08  [Refereed]

     View Summary

    The impact of posture on the immediate recovery of intravascular fluid and protein after intense exercise was determined in 14 volunteers. Forces which govern fluid and protein movement in muscle interstitial fluid pressure (P-ISF), interstitial colloid osmotic pressure (COPi), and plasma colloid osmotic pressure (COPi) were measured before and after exercise in the supine or upright position. During exercise, plasma volume (PV) decreased by 5.7 +/- 0.7 and 7.0 +/- 0.5 ml/kg body weight in the supine and upright posture, respectively. During recovery, PV returned to its baseline value within 30 min regardless of posture. PV fell below this level by 60 and 120 min in the supine and upright posture, respectively (P &lt; 0.05). Maintenance of PV in the upright position was associated with a decrease in systolic blood pressure, an increase in COPp (from 25 +/- 1 to 27 +/- 1 mmHg; P &lt; 0.05), and an increase in P-ISF (from 5 +/- 1 to 6 +/-: 2 mmHg), whereas COPi was unchanged. Increased PISF indicates that the hydrostatic pressure gradient favors fluid movement into the vascular space. However, retention of the recaptured fluid in the plasma is promoted only in the upright posture because of increased COPi.

  • Influence of hydrostatic pressure gradients on regulation of plasma volume after exercise

    GW Mack, R Yang, AR Hargens, K Nagashima, A Haskell

    JOURNAL OF APPLIED PHYSIOLOGY   85 ( 2 ) 667 - 675  1998.08

     View Summary

    The impact of posture on the immediate recovery of intravascular fluid and protein after intense exercise was determined in 14 volunteers. Forces which govern fluid and protein movement in muscle interstitial fluid pressure (P-ISF), interstitial colloid osmotic pressure (COPi), and plasma colloid osmotic pressure (COPi) were measured before and after exercise in the supine or upright position. During exercise, plasma volume (PV) decreased by 5.7 +/- 0.7 and 7.0 +/- 0.5 ml/kg body weight in the supine and upright posture, respectively. During recovery, PV returned to its baseline value within 30 min regardless of posture. PV fell below this level by 60 and 120 min in the supine and upright posture, respectively (P &lt; 0.05). Maintenance of PV in the upright position was associated with a decrease in systolic blood pressure, an increase in COPp (from 25 +/- 1 to 27 +/- 1 mmHg; P &lt; 0.05), and an increase in P-ISF (from 5 +/- 1 to 6 +/-: 2 mmHg), whereas COPi was unchanged. Increased PISF indicates that the hydrostatic pressure gradient favors fluid movement into the vascular space. However, retention of the recaptured fluid in the plasma is promoted only in the upright posture because of increased COPi.

  • Effects of posture on cardiovascular responses to lower body positive pressure at rest and during dynamic exercise

    T Nishiyasu, K Nagashima, ER Nadel, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   85 ( 1 ) 160 - 167  1998.07  [Refereed]

     View Summary

    We tested the hypothesis that cardiovascular responses to lower body positive pressure (LBPP) would be dependent on the posture of the subject and also on the background condition (rest or exercise). We measured heart rate (HR), mean arterial blood pressure (MAP), and cardiac stroke volume in eight subjects at rest and during cycle ergometer exercise (76 +/- 3 W) with and without LBPP (25, 50, and 75 mmHg) in the supine and upright positions. At rest, the increase in MAP was proportional to the increase in LBPP and was greater in the supine (6 +/- 2, 15 +/- 3, and 26 +/- 3 mmHg) than in the upright (2 +/- 3, 9 +/- 3, and 17 +/- 3 mmHg) position. During dynamic exercise, the increases in MAP evoked by 25, 50, and 75 mmHg LBPP were greater in the supine (13 +/- 2, 28 +/- 3, and 40 +/- 3 mmHg) than in the upright (7 +/- 3, 12 +/- 3, and 25 +/- 3 mmHg) position. We conclude that the systemic pressure response to LBPP is clearly dependent on the body position, with the larger pressure responses being associated with the supine position both at rest and during dynamic leg exercise.

  • Role of plasma osmolality in the delayed onset of thermal cutaneous vasodilation during exercise in humans

    A Takamata, K Nagashima, H Nose, T Morimoto

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   275 ( 1 ) R286 - R290  1998.07  [Refereed]

     View Summary

    To elucidate the role of increased plasma osmolality (P-osmol), which occurs during exercise in the regulation of cutaneous vasodilation (CVD) during exercise, we determined the relationship between the change in esophageal temperature (Delta T-es) required to elicit CVD (Delta T-es threshold for CVD) and P-osmol during light and moderate exercise (30 and 55% of peak oxygen consumption, respectively) and passive body heating. Then we compared the relationship with the data obtained in our previous study [A. Takamata, K. Nagashima, H. Nose, and T. Morimoto.Am. J. Physiol. 273 (Regulatory Integrative Comp. Physiol. 42): R197-R204, 1997], in which we determined the relationships during passive body heating following isotonic (0.9% NaCl) or hypertonic (2 or 3% NaCl) saline infusions in the same subjects. P-osmol values at 5 min after the onset of exercise were 287.5 +/- 0.9 mosmol/kgH(2)O during light exercise and 293.0 +/- 1.2 mosmol/kgH(2)O during moderate exercise. P-osmol just before passive body heating was 289.9 +/- 1.4 mosmol/kgH(2)O. The Delta T-es threshold for CVD was 0.09 +/- 0.05 degrees C during light exercise, 0.31 +/- 0.09 degrees C during moderate exercise, and 0.10 +/- 0.05 degrees C during passive body heating. The relationship between the Delta T-es threshold for CVD and P-osmol was shown to be on the same regression line both during exercise and during passive body heating with or without infusions [A. Takamata, K. Nagashima, H. Nose, and T. Morimoto. Am. J. Physiol. 273 (Regulatory Integrative Comp. Physiol. 42): R197-R204, 1997]. Our data suggest that the elevated body core temperature threshold for CVD during exercise could be the result of increased P-osmol induced by exercise and is not due to reduced plasma volume or the intensity of the exercise itself.

  • Role of plasma osmolality in the delayed onset of thermal cutaneous vasodilation during exercise in humans

    A Takamata, K Nagashima, H Nose, T Morimoto

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   275 ( 1 ) R286 - R290  1998.07

     View Summary

    To elucidate the role of increased plasma osmolality (P-osmol), which occurs during exercise in the regulation of cutaneous vasodilation (CVD) during exercise, we determined the relationship between the change in esophageal temperature (Delta T-es) required to elicit CVD (Delta T-es threshold for CVD) and P-osmol during light and moderate exercise (30 and 55% of peak oxygen consumption, respectively) and passive body heating. Then we compared the relationship with the data obtained in our previous study [A. Takamata, K. Nagashima, H. Nose, and T. Morimoto.Am. J. Physiol. 273 (Regulatory Integrative Comp. Physiol. 42): R197-R204, 1997], in which we determined the relationships during passive body heating following isotonic (0.9% NaCl) or hypertonic (2 or 3% NaCl) saline infusions in the same subjects. P-osmol values at 5 min after the onset of exercise were 287.5 +/- 0.9 mosmol/kgH(2)O during light exercise and 293.0 +/- 1.2 mosmol/kgH(2)O during moderate exercise. P-osmol just before passive body heating was 289.9 +/- 1.4 mosmol/kgH(2)O. The Delta T-es threshold for CVD was 0.09 +/- 0.05 degrees C during light exercise, 0.31 +/- 0.09 degrees C during moderate exercise, and 0.10 +/- 0.05 degrees C during passive body heating. The relationship between the Delta T-es threshold for CVD and P-osmol was shown to be on the same regression line both during exercise and during passive body heating with or without infusions [A. Takamata, K. Nagashima, H. Nose, and T. Morimoto. Am. J. Physiol. 273 (Regulatory Integrative Comp. Physiol. 42): R197-R204, 1997]. Our data suggest that the elevated body core temperature threshold for CVD during exercise could be the result of increased P-osmol induced by exercise and is not due to reduced plasma volume or the intensity of the exercise itself.

  • Effects of posture on cardiovascular responses to lower body positive pressure at rest and during dynamic exercise

    T Nishiyasu, K Nagashima, ER Nadel, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   85 ( 1 ) 160 - 167  1998.07

     View Summary

    We tested the hypothesis that cardiovascular responses to lower body positive pressure (LBPP) would be dependent on the posture of the subject and also on the background condition (rest or exercise). We measured heart rate (HR), mean arterial blood pressure (MAP), and cardiac stroke volume in eight subjects at rest and during cycle ergometer exercise (76 +/- 3 W) with and without LBPP (25, 50, and 75 mmHg) in the supine and upright positions. At rest, the increase in MAP was proportional to the increase in LBPP and was greater in the supine (6 +/- 2, 15 +/- 3, and 26 +/- 3 mmHg) than in the upright (2 +/- 3, 9 +/- 3, and 17 +/- 3 mmHg) position. During dynamic exercise, the increases in MAP evoked by 25, 50, and 75 mmHg LBPP were greater in the supine (13 +/- 2, 28 +/- 3, and 40 +/- 3 mmHg) than in the upright (7 +/- 3, 12 +/- 3, and 25 +/- 3 mmHg) position. We conclude that the systemic pressure response to LBPP is clearly dependent on the body position, with the larger pressure responses being associated with the supine position both at rest and during dynamic leg exercise.

  • Effect of continuous negative-pressure breathing on skin blood flow during exercise in a hot environment

    K Nagashima, H Nose, A Takamata, T Morimoto

    JOURNAL OF APPLIED PHYSIOLOGY   84 ( 6 ) 1845 - 1851  1998.06  [Refereed]

     View Summary

    To assess the impact of continuous negative-pressure breathing (CNPB) on the regulation of skin blood flow, we measured forearm blood flow (FBF) by venous-occlusion plethysmography and laser-Doppler flow (LDF) at the anterior chest during exercise in a hot environment (ambient temperature = 30 degrees C, relative humidity = similar to 30%). Seven male subjects exercised in the upright position at an intensity of 60% peak oxygen consumption rate for 40 min with and without CNPB after 20 min of exercise. The esophageal temperature (T-es) in both conditions increased to 38.1 degrees C by the end of exercise, without any significant differences between the two trials. Mean arterial pressure (MAP) increased by similar to 15 mmHg by 8 min of exercise, without any significant difference between the two trials before CNPB. However, CNPB reduced MAP by similar to 10 mmHg after 24 min of exercise (P &lt; 0.05). The increase in FBF and LDF in the control condition leveled off after 18 min of exercise above a T-es of 37.7 degrees C, whereas in the CNPB trial the increase continued, with a rise in T-es despite the decrease in MAP. These results suggest that CNPB enhances vasodilation of skin above a T-es of similar to 38 degrees C by stretching intrathoracic baroreceptors such as cardiopulmonary baroreceptors.

  • Effect of continuous negative-pressure breathing on skin blood flow during exercise in a hot environment

    K Nagashima, H Nose, A Takamata, T Morimoto

    JOURNAL OF APPLIED PHYSIOLOGY   84 ( 6 ) 1845 - 1851  1998.06

     View Summary

    To assess the impact of continuous negative-pressure breathing (CNPB) on the regulation of skin blood flow, we measured forearm blood flow (FBF) by venous-occlusion plethysmography and laser-Doppler flow (LDF) at the anterior chest during exercise in a hot environment (ambient temperature = 30 degrees C, relative humidity = similar to 30%). Seven male subjects exercised in the upright position at an intensity of 60% peak oxygen consumption rate for 40 min with and without CNPB after 20 min of exercise. The esophageal temperature (T-es) in both conditions increased to 38.1 degrees C by the end of exercise, without any significant differences between the two trials. Mean arterial pressure (MAP) increased by similar to 15 mmHg by 8 min of exercise, without any significant difference between the two trials before CNPB. However, CNPB reduced MAP by similar to 10 mmHg after 24 min of exercise (P &lt; 0.05). The increase in FBF and LDF in the control condition leveled off after 18 min of exercise above a T-es of 37.7 degrees C, whereas in the CNPB trial the increase continued, with a rise in T-es despite the decrease in MAP. These results suggest that CNPB enhances vasodilation of skin above a T-es of similar to 38 degrees C by stretching intrathoracic baroreceptors such as cardiopulmonary baroreceptors.

  • Nonshivering thermoregulatory responses in trained athletes: Effects of physical fitness and body fat

    T Yoshida, K Nagashima, S Nakai, A Yorimoto, T Kawabata, T Morimoto

    JAPANESE JOURNAL OF PHYSIOLOGY   48 ( 2 ) 143 - 148  1998.04  [Refereed]

     View Summary

    We studied the difference of thermoregulatory responses between trained male athletes (TR, n = 9) and untrained men (UT, n = 7) during 60 min of cold exposure (15 degrees C) without shivering, and examined the effects of physical fitness and body fat on these responses. Mean skin temperature ((T) over bar(sk)), esophageal temperature (T-es), and skin conductance (K-b) were similar between TR and UT, and heat production ((M) over bar) for TR increased significantly during exposure at 15 degrees C. The (M) over bar at 15 degrees C correlated positively with maximal oxygen uptake and negatively with body fat (%BF), but not with T-es. The K-b correlated negatively with T-es and positively with (T) over bar(sk).The %BF also correlated negatively with K-b and (T) over bar(sk) during exposure at 15 degrees C, and the slope of %BF vs. (T) over bar(sk) relationship was significantly steeper in TR than in UT. These results suggest that (1) body temperature is maintained by the reduction of skin conductance, and (2) heat insulation independent of body fat is enhanced in trained athletes during cold exposure without shivering.

    DOI

  • Renal sodium handling after exercise induced plasma volume expansion.

    SA Kavouras, K Nagashima, J Wu, RD Wemple, ER Nadel, GW Mack

    FASEB JOURNAL   12 ( 5 ) A1117 - A1117  1998.03  [Refereed]

  • The role of albumin in the hypervolemia of exercise "jointly worked"

    The 1997 Nagano Symposium on Sports Science     375 - 384  1998

  • Role of plasma osmolality in the exercise-induced inhibition of cutaneous vasodilation "jointly worked"

    ibid Section 6     344 - 348  1998

  • Cardiac filling and lactate threshold during graded exercise "jointly worked"

    The 5th Annu al Pacific Rim Conference on Exercise Science and Sports Medicine; Exercise and the Human Body     209 - 219  1998

  • Cardiac filling and lactate concentration in plasma during exercise in a hot environment "jointly worked"

    ibid Section 6   40   306 - 316  1998

  • Cardiac filling and lactate concentration in plasma during exercise in a hot environment "jointly worked"

    The 1997 Nagano Symposium on Sports Sciences     231 - 234  1998

  • Transcapillary escape rate of albumin in humans during exercise-induced hypervolemia

    A Haskell, ER Nadel, NS Stachenfeld, K Nagashima, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   83 ( 2 ) 407 - 413  1997.08  [Refereed]

     View Summary

    To test the hypotheses that plasma volume (PV) expansion 24 h after intense exercise is associated with reduced transcapillary escape rate of albumin (TERalb) and that local changes in transcapillary forces in the previously active tissues favor retention of protein in the vascular space, we measured PV, TERalb, plasma colloid osmotic pressure (COPp), interstitial fluid hydrostatic pressure (Pi), and colloid osmotic pressure in leg muscle and skin and capillary filtration coefficient (CFC) in the arm and leg in seven men and women before and 24 h after intense upright cycle ergometer exercise. Exercise expanded PV by 6.4% at 24 h (43.9 +/- 0.8 to 46.8 +/- 1.2 ml/kg, P &lt; 0.05) and decreased total protein concentration (6.5 +/- 0.1 to 6.3 +/- 0.1 g/dl, P &lt; 0.05) and COPp (26.1 +/- 0.8 to 24.3 +/- 0.9 mmHg, P &lt; 0.05), although plasma albumin concentration was unchanged. TERalb tended to decline (8.4 +/- 0.5 to 6.5 +/- 0.7%/h, P = 0.11) and was correlated with the increase in PV (r = -0.69, P &lt; 0.05). CFC increased in the leg (3.2 +/- 0.2 to 4.3 +/- 0.5 mu l.100 g(-1).min(-1).mmHg(-1), P &lt; 0.05), and Pi showed a trend to increase in the leg muscle (2.8 +/- 0.7 to 3.8 +/- 0.3 mmHg, P = 0.08). These data demonstrate that TERalb is associated with PV regulation and that local transcapillary forces in the leg muscle may favor retention of albumin in the vascular space after exercise.

  • Transcapillary escape rate of albumin in humans during exercise-induced hypervolemia

    A Haskell, ER Nadel, NS Stachenfeld, K Nagashima, GW Mack

    JOURNAL OF APPLIED PHYSIOLOGY   83 ( 2 ) 407 - 413  1997.08

     View Summary

    To test the hypotheses that plasma volume (PV) expansion 24 h after intense exercise is associated with reduced transcapillary escape rate of albumin (TERalb) and that local changes in transcapillary forces in the previously active tissues favor retention of protein in the vascular space, we measured PV, TERalb, plasma colloid osmotic pressure (COPp), interstitial fluid hydrostatic pressure (Pi), and colloid osmotic pressure in leg muscle and skin and capillary filtration coefficient (CFC) in the arm and leg in seven men and women before and 24 h after intense upright cycle ergometer exercise. Exercise expanded PV by 6.4% at 24 h (43.9 +/- 0.8 to 46.8 +/- 1.2 ml/kg, P &lt; 0.05) and decreased total protein concentration (6.5 +/- 0.1 to 6.3 +/- 0.1 g/dl, P &lt; 0.05) and COPp (26.1 +/- 0.8 to 24.3 +/- 0.9 mmHg, P &lt; 0.05), although plasma albumin concentration was unchanged. TERalb tended to decline (8.4 +/- 0.5 to 6.5 +/- 0.7%/h, P = 0.11) and was correlated with the increase in PV (r = -0.69, P &lt; 0.05). CFC increased in the leg (3.2 +/- 0.2 to 4.3 +/- 0.5 mu l.100 g(-1).min(-1).mmHg(-1), P &lt; 0.05), and Pi showed a trend to increase in the leg muscle (2.8 +/- 0.7 to 3.8 +/- 0.3 mmHg, P = 0.08). These data demonstrate that TERalb is associated with PV regulation and that local transcapillary forces in the leg muscle may favor retention of albumin in the vascular space after exercise.

  • Osmoregulatory inhibition of thermally induced cutaneous vasodilation in passively heated humans

    A Takamata, K Nagashima, H Nose, T Morimoto

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   273 ( 1 ) R197 - R204  1997.07  [Refereed]

     View Summary

    We examined the effect of increased plasma osmolality (P-osm) on cutaneous vasodilatory response to increased esophageal temperature (T-es) in passively heated human subjects (n = 6). To modify P-osm, subjects were infused with 0.9, 2, or 3% NaCl infusions (Inf) for 90 min on separate days. Infusion rates were 0.2, 0.15, and 0.125 ml . min(-1). kg body wt(-1) for 0.9, 2, and 3% Inf, respectively, which produced relatively similar plasma volume expansion. Thirty minutes after the end of infusion, subjects immersed their lower legs in a water bath at 42 degrees C (room temperature 28 degrees C) for 60 min after 10 min of preheating control measurements. Passive heating without infusion (NI) served as time control to account for the effect of volume expansion. P-osm (mosmol/kgH(2)O) values at the onset of passive heating were 289.9 +/- 1.4, 292.1 +/- 0.6, 298.7 +/- 0.7, and 305.6 +/- 0.6 after NI, 0.9% Inf, 2% Inf, and 3% Inf, respectively. The increases in T-es (Delta T-es) at equilibrium during passive heating (mean Delta T-es during 55-60 min) were 0.47 +/- 0.08, 0.59 +/- 0.08, 0.85 +/- 0.13, and 1.09 +/- 0.12 degrees C after NI, 0.9% Inf, 2% Inf, and 3% Inf, respectively, which indicates that T-es at equilibrium increased linearly as P-osm increased. Delta T-es required to elicit cutaneous vasodilation (Delta T-es threshold for cutaneous vasodilation) also increased linearly as P-osm increased as well as the Delta T-es threshold for sweating. The calculated increases in these thresholds per unit rise in P-osm from regression analysis were 0.044 degrees C for the cutaneous vasodilation and 0.034 degrees C for sweating. Thus the Delta T-es thresholds for cutaneous vasodilation and sweating are shifted to higher Delta T-es along with the increase in P-osm, and these shifts resulted in the higher increase in T-es during passive heating.

  • Relationship between aerobic power, blood volume, and thermoregulatory responses to exercise-heat stress

    T Yoshida, K Nagashima, H Nose, T Kawabata, S Nakai, A Yorimoto, T Morimoto

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   29 ( 7 ) 867 - 873  1997.07  [Refereed]

     View Summary

    To clarify the relationship between aerobic power ((V) over dot O-2max), blood volume (BV), and thermoregulatory responses to exercise-heat stress, we analyzed the cross-sectional relationship between the resting BV, plasma volume (PV), erythrocyte volume (EV), (V) over dot O-2max, forearm blood flow (FBF), and sweating responses during exercise in a hot environment (31 degrees C, 50% relative humidity). Twelve college-aged male subjects with a mean maximal oxygen uptake of 48 (range 42-59) mL.kg(-1).min(-1), a mean PV of 54 (range 42-72) mL.kg(-1), a mean EV of 31 (range 23-43) mL.kg(-1), and a mean BV of 85 (range 67-115) mL.kg(-1) (measured by the Evans Blue dye dilution method) performed three sessions of 20-min cycle exercise at two levels of intensity (40% and 60% (V) over dot O-2max). The BV, PV, and EV correlated positively with peak FBF (r = 0.596-0.711, P &lt; 0.05), the increase of FBF in response to a unit rise in esophageal temperature (Tes; peak Delta FBF/peak Delta Tes) (r = 0.592-0.656, P &lt; 0.05) and with total sweat loss (TSL) (r = 0.599-0.634, P &lt; 0.05) during the exercise. The (V) over dot O-2max correlated with TSL during exercise at 40% (V) over dot O-2max (r = 0.578, P &lt; 0.05), but not with peak FBF and peak Delta FBF/peak Delta Tes. The (V) over dot O-2max per lean body mass also showed a significant positive correlation with BV (r = 0.769, P &lt; 0.01), PV (r = 0.706, P &lt; 0.05), and with EV (r = 0.841, P &lt; 0.001). The peak Delta FBF/peak Delta Tes was correlated positively with peak FBF (r = 0.597-0.830, P &lt; 0.05-0.01) and negatively with peak Tes (r = 0.641-0.769, P &lt; 0.05-0.01) during the exercise at the two levels. However, the chest sweat rate (CSR), TSL, and the increase of CSR in response to a unit rise in Tes (peak Delta CSR/peak Delta Tes) showed no correlation with peak Tes during the exercise at the two levels. These findings suggest that 1) heat dissipation responses during exercise were related more to blood volume than aerobic power and 2) skin blood flow was related more to body temperature than sweating responses during exercise under mild heat stress.

    DOI

  • Osmoregulatory inhibition of thermally induced cutaneous vasodilation in passively heated humans

    A Takamata, K Nagashima, H Nose, T Morimoto

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   273 ( 1 ) R197 - R204  1997.07

     View Summary

    We examined the effect of increased plasma osmolality (P-osm) on cutaneous vasodilatory response to increased esophageal temperature (T-es) in passively heated human subjects (n = 6). To modify P-osm, subjects were infused with 0.9, 2, or 3% NaCl infusions (Inf) for 90 min on separate days. Infusion rates were 0.2, 0.15, and 0.125 ml . min(-1). kg body wt(-1) for 0.9, 2, and 3% Inf, respectively, which produced relatively similar plasma volume expansion. Thirty minutes after the end of infusion, subjects immersed their lower legs in a water bath at 42 degrees C (room temperature 28 degrees C) for 60 min after 10 min of preheating control measurements. Passive heating without infusion (NI) served as time control to account for the effect of volume expansion. P-osm (mosmol/kgH(2)O) values at the onset of passive heating were 289.9 +/- 1.4, 292.1 +/- 0.6, 298.7 +/- 0.7, and 305.6 +/- 0.6 after NI, 0.9% Inf, 2% Inf, and 3% Inf, respectively. The increases in T-es (Delta T-es) at equilibrium during passive heating (mean Delta T-es during 55-60 min) were 0.47 +/- 0.08, 0.59 +/- 0.08, 0.85 +/- 0.13, and 1.09 +/- 0.12 degrees C after NI, 0.9% Inf, 2% Inf, and 3% Inf, respectively, which indicates that T-es at equilibrium increased linearly as P-osm increased. Delta T-es required to elicit cutaneous vasodilation (Delta T-es threshold for cutaneous vasodilation) also increased linearly as P-osm increased as well as the Delta T-es threshold for sweating. The calculated increases in these thresholds per unit rise in P-osm from regression analysis were 0.044 degrees C for the cutaneous vasodilation and 0.034 degrees C for sweating. Thus the Delta T-es thresholds for cutaneous vasodilation and sweating are shifted to higher Delta T-es along with the increase in P-osm, and these shifts resulted in the higher increase in T-es during passive heating.

  • The mechanism underlying increased plasma albumin content associated with exercise-induced hypervolemia

    K Nagashima, GW Cline, GW Mack, GI Shulman, ER Nadel

    FASEB JOURNAL   11 ( 3 ) 301 - 301  1997.02  [Refereed]

  • Negative pressure breathing and the control of skin blood flow during exercise in a hot environment

    K Nagashima, T Yoshida, H Nose, A Takamata, T Morimoto

    THERMOREGULATION   813   604 - 609  1997  [Refereed]

    DOI

  • Negative pressure breathing and the control of skin blood flow during exercise in a hot environment

    K Nagashima, T Yoshida, H Nose, A Takamata, T Morimoto

    THERMOREGULATION   813 ( 1 Thermoregulat ) 604 - 609  1997  [Refereed]

    DOI

  • Osmotic inhibition of thermally induced cutaneous vasodilation in humans and its role in the inhibition of cutaneous vasodilation during exercise "jointly worked"

    Thermal Physiology 1997     99 - 102  1997

  • Negative pressure breathing and the control of skin blood flow during exercise in a hot environment "jointly worked"

    Thermoregulation     604 - 609  1997

  • Effect of hydration level on thermoregulatory responses during exercise in rats "jointly worked"

    Thermal Physiology 1997     103 - 106  1997

  • Effect of plasma osmolality on thermoregulatory responses to passive heating in humans

    A Takamata, K Nagashima, H Nose, T Morimoto

    FASEB JOURNAL   10 ( 3 ) 681 - 681  1996.03  [Refereed]

  • Cardiovascular response to exercise in a hot environment "jointly worked"

    Progress in Biometeology   11   13 - 22  1996

  • RELATIONSHIP BETWEEN ATRIAL-NATRIURETIC-PEPTIDE AND PLASMA-VOLUME DURING GRADED-EXERCISE WITH WATER IMMERSION

    K NAGASHIMA, H NOSE, T YOSHIDA, T KAWABATA, Y ODA, A YORIMOTO, O UEMURA, T MORIMOTO

    JOURNAL OF APPLIED PHYSIOLOGY   78 ( 1 ) 217 - 224  1995.01  [Refereed]

     View Summary

    To assess the relationship between atrial natriuretic peptide (ANP) and the reduction in plasma volume (PV) during exercise, we measured changes in PV and ANP in seven male volunteers during treadmill exercise in air (AE) and with water immersion (WE) together with time control studies of rest in air and in water. Blood samples were collected from a catheter in the antecubital vein at exercise intensities of 32, 49, 65, and 78% of peak oxygen consumption (Vo(2)). Plasma ANP in AE increased significantly from the resting value [15 +/- 1 (SE) pg/ml] only at 78% of peak Vo(2) (29 +/- 5 pg/ml), whereas ANP in WE increased significantly at exercise levels of &gt;49% of peak Vo(2) and reached 68 +/- 9 pg/ml at 78% of peak Vo(2). Although PV in AE and WE decreased significantly with Vo(2) of &gt; 49% of peak Vo(2) (P &lt; 0.01), the decrease from the resting value in WE was significantly greater than that in AE of &gt;65% of peak Vo(2) (P &lt; 0.01) and the decreases at 78% of peak Vo(2) were -9.7 +/- 0.8 and -6.1 +/- 1.7%, respectively. The difference in the decrease in PV between AE and WE at corresponding Vo(2) correlated strongly with that in the increase in ANP (r = -0.97; P &lt; 0.01). These results are consistent with the hypothesis that ANP may be involved in the fluid shift from the intra- to extravascular space during exercise.

  • 左心室の弾性測定における容積変化率依存性(共著)

    循環器科   37   524 - 527  1995

  • EFFECTS OF ALPHA-ADRENERGIC ACTIVATION ON THE SHIFT OF ELECTROLYTES AND FLUID AFTER HEMORRHAGE IN RATS

    K NAGASHIMA

    JAPANESE JOURNAL OF PHYSIOLOGY   45 ( 3 ) 381 - 395  1995  [Refereed]

     View Summary

    To assess the effects of alpha-adrenergic activation on hemorrhage-induced shifts of K+, Na+ and fluid between the intravascular and extravascular spaces, we continuously measured changes in plasma concentrations of K+ and Na+ (Delta[K+]p, Delta[Na+]p) and blood volume (Delta BV) over 60 min after hemorrhage in nephrectomized rats. Hemorrhage was conducted over 5 min at the level of 0.5, 1.0 and 1.5% of body weight (H-0.5, H-1.0, H-1.5), and the result was compared with hemorrhage of 1.0% body weight after administration of alpha(1)-adrenoreceptor antagonist prazosin (0.2 mg/kg, H-1.0P). Delta[K+]p increased significantly (p &lt; 0.05) after hemorrhage, and the peak value was proportional to the level of hemorrhage (0.18 +/- 0.08, 0.62 +/- 0.22 and 1.64 +/- 0.19 meq/l in H-0.5, H-1.0 and H-1.5, respectively). Delta[Na+]p decreased significantly (p &lt; 0.05) after hemorrhage, and the decrease was sustained until the end of the experiment (-0.8 +/- 0.6, -1.0 +/- 0.5 and -2.2 +/- 0.5 meq/l in H-0.5, H-1.0 and H-1.5, respectively). In H-1.0P, the increase in Delta[K+]p (0.25 +/- 0.09 meq/l at the peak) and the decrease in Delta[Na+]p (-0.2 +/- 0.1 meq/l at the bottom) were suppressed significantly (p &lt; 0.05) compared to H-1.0. Although Delta BV was greater in H-1.0P than H-1.0, plasma K+ content was not different between the groups. In H-1.0, the calculated concentrations of K+ and Na+ in the fluid which shifted into the intravascular space ([K+]sf, [Na+]sf) in the first 30 min after hemorrhage were higher in [K+]sf (6.25 +/- 0.70 meq/l) and lower in [Na+]sf (128.0 +/- 3.2 meq/l) than the pre-hemorrhage plasma level. With regard to H-1.0P, [K+]sf and [Na+]sf were not different from the pre-hemorrhage level of plasma. These results suggest that alpha-adrenergic activation after hemorrhage induces K+ movement into plasma to increase [K+]p, which might be related to the fluid shift from the intracellular to the extracellular space.

    DOI

  • RELATIONSHIP BETWEEN ATRIAL-NATRIURETIC-PEPTIDE AND PLASMA-VOLUME DURING GRADED-EXERCISE WITH WATER IMMERSION

    K NAGASHIMA, H NOSE, T YOSHIDA, T KAWABATA, Y ODA, A YORIMOTO, O UEMURA, T MORIMOTO

    JOURNAL OF APPLIED PHYSIOLOGY   78 ( 1 ) 217 - 224  1995.01

     View Summary

    To assess the relationship between atrial natriuretic peptide (ANP) and the reduction in plasma volume (PV) during exercise, we measured changes in PV and ANP in seven male volunteers during treadmill exercise in air (AE) and with water immersion (WE) together with time control studies of rest in air and in water. Blood samples were collected from a catheter in the antecubital vein at exercise intensities of 32, 49, 65, and 78% of peak oxygen consumption (Vo(2)). Plasma ANP in AE increased significantly from the resting value [15 +/- 1 (SE) pg/ml] only at 78% of peak Vo(2) (29 +/- 5 pg/ml), whereas ANP in WE increased significantly at exercise levels of &gt;49% of peak Vo(2) and reached 68 +/- 9 pg/ml at 78% of peak Vo(2). Although PV in AE and WE decreased significantly with Vo(2) of &gt; 49% of peak Vo(2) (P &lt; 0.01), the decrease from the resting value in WE was significantly greater than that in AE of &gt;65% of peak Vo(2) (P &lt; 0.01) and the decreases at 78% of peak Vo(2) were -9.7 +/- 0.8 and -6.1 +/- 1.7%, respectively. The difference in the decrease in PV between AE and WE at corresponding Vo(2) correlated strongly with that in the increase in ANP (r = -0.97; P &lt; 0.01). These results are consistent with the hypothesis that ANP may be involved in the fluid shift from the intra- to extravascular space during exercise.

  • 術前貯血法へのエリスロポイエチンの応用と問題点-合併症ならびに年齢体重からみた投与基準の検討(共著)

    自己血輸血   6   152 - 157  1992

  • ERYTHROPOIETIN投与下の骨髄の検討(共著)

    自己血輸血   5   78 - 82  1992

  • SUCCESSFUL CORRECTION OF A COLLETT EDWARDS TYPE-II TRUNCUS ARTERIOSUS AFTER BILATERAL PULMONARY-ARTERY BANDING - A CASE-REPORT

    Y WADA, T KAWAI, K NISHIYAMA, K NAGASHIMA, S SHIRAKATA, K OGA, T OKA

    JAPANESE JOURNAL OF SURGERY   19 ( 6 ) 751 - 755  1989.11  [Refereed]

    DOI DOI2

  • SUCCESSFUL CORRECTION OF A COLLETT EDWARDS TYPE-II TRUNCUS ARTERIOSUS AFTER BILATERAL PULMONARY-ARTERY BANDING - A CASE-REPORT

    Y WADA, T KAWAI, K NISHIYAMA, K NAGASHIMA, S SHIRAKATA, K OGA, T OKA

    JAPANESE JOURNAL OF SURGERY   19 ( 6 ) 751 - 755  1989.11  [Refereed]

    DOI

  • 転移性肝癌に対する肝切除の検討(共著)

    交通医学   43   165 - 171  1989

  • 高齢者胃癌患者の術前、術後の合併症について(共著)

    交通医学   43   160 - 163  1989

  • INFILTRATING DUCTAL CARCINOMA DEVELOPING WITHIN CYSTOSARCOMA PHYLLODES - A CASE-REPORT

    T YASUMURA, S MATSUI, T HAMAJIMA, K NAGASHIMA, H YAMAGISHI, AIKAWA, I, T OKA, T NAKAE, N SHIMADA

    JAPANESE JOURNAL OF SURGERY   18 ( 3 ) 326 - 329  1988.05  [Refereed]

    DOI DOI2

  • 術後癌性腹膜炎に対するCDDP,OK-432の腹腔内同時投与の治療効果(共著)

    交通医学   42   298 - 301  1988

  • Infiltrating ductal carcinoma developing within Cystosarcoma Phyllodes “jointly worked”

    Yasumura T, Matsui S, Hamajima T, Nagashima K, Yamagishi H, Aikawa I, Oka T, Nakae T, Shimada N

    Japanese Journal of Surgery   18 ( 3 ) 326 - 329  1988  [Refereed]

    DOI

  • Family Academy

    2014 栄冠めざして Family     52 - 55

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Books and Other Publications

  • 高校健保ニュース 「熱中症から体を守る 暑熱順化」

    永島計(P1)

    少年写真新聞  2022.05

  • Body Temperature Physiology for understanding Why

    Kei Nagashima( Part: Edit)

    2021.04

  • 健康の科学知識 : 食事、睡眠、美容、体調 ……、知って役立つ体の知識50

    ニュートンプレス

    ニュートンプレス  2021.01 ISBN: 9784315523096

  • 40℃超えの日本列島でヒトは生きていけるのか 体温の科学から学ぶ猛暑のサバイバル術

    永島 計( Part: Sole author)

    化学同人  2019.07 ISBN: 9784759816822

  • 快適な温熱環境のしくみと実践

    永島 計(脳と体温調節)

    公益社団法人 空気調和・衛生工学会  2019.03

  • 体の科学知識体質編

    永島 計( Part: Other, 熱はどうやってつくられる?なぜ上下する?)

    株式会社 ニュートンプレス  2018.12

  • ニュー運動生理学Ⅱ(共著)

    宮村美晴編

    真興交易株式会社、医書出版部  2015.01

  • ぬくぬくげんきぼくのたいおん

    永島 計

    少年写真新聞社  2014.11 ISBN: 9784879815026

  • ニュー運動生理学(共著)

    永島計

    真興貿易  2014

  • 小児内科

    永島計

    東京医学社  2014

  • スタンダード生理学第3版(編著)

    二宮石雄, 安藤啓司, 彼末一之, 松川寛二編集

    文光堂  2013.09 ISBN: 9784830602276

  • Wilderness Medicine

    Paul S. Auerbach

    Mosby  2011.12 ISBN: 9781437716788

  • 体温II

    井上 芳光, 近藤 徳彦

    NAP  2010.12 ISBN: 9784931411005

  • からだと温度の辞典

    彼末一之監修

    朝倉書店  2010.04

  • からだと温度の事典

    永島 計, 紫藤 治, 稲葉 裕, 田村 照子, 太田 俊二, 堀越 哲美, 澤田 晋一, 田中 英登, 福岡 義隆, 彼末 一之

    朝倉書店  2010 ISBN: 9784254301021

  • ホメオスタシスの科学

    医学映像教育センター, 永島 計

    医学映像教育センター  2010 ISBN: 9784862434029

  • 運動と免疫

    大野秀樹, 木崎節子

    NAP  2009.09 ISBN: 9784931411883

  • 身体トレーニング

    共著

    真興交易(株)医書出版部  2009.03 ISBN: 9784880038261

  • 「健康福祉」 人間科学(編著)

    朝倉書店  2008.06

  • 解体新ショー(監修)

    NHK出版  2008.06

  • 健康運動指導マニュアル(編著)

    文光堂  2008.02

  • 環境生理学(共著)

    北海道大学出版会  2007

  • スタンダード生理学第二版(編著)

    文光堂  2007

  • 絶食時の体温概日リズム変調の解析

    永島 計

    [永島計]  2004

  • 体温

    平田 耕造, 近藤 徳彦, 井上 芳光

    NAP  2002.09 ISBN: 9784931411319

  • スタンダード生理学(編著)

    文光堂  2002

  • 新運動生理学(編著)

    真興交易  2001

  • The Central Organization of the Thermoregulatory System Thermotherapy for Neoplasia Inflammation, and Pain

    編著

    2001

  • 運動と遺伝(編著)

    大修館書店  2001

  • The role of albumin in the hypervolemia of exercise. In The 1997 Nagano Symposium on Sports Science

    編著

    Cooper Publishing  1998

  • Cardiac filling and lactate threshold during graded exercise The 5th Annual Pacific Rim Conference on Exercise Science and Sports Medicine; Exercise and the Human Body

    編著

    Chosun-University  1998

  • Cardiac filling and lactate concentration in plasma during exercise in a hot environment

    編著

    1998

  • Role of plasma osmolality in the exercise-induced inhibition of cutaneous vasodilation

    編著

    1998

  • Cardiac filling and lactate concentration in plasma during exercise in a hot environment The 1997 Nagano Symposium on Sports Sciences

    I. L. Cooper  1998

  • Negative pressure breathing and the control of skin blood flow during exercise in a hot environment Thermoregulation

    編著

    Annals of the New York Academy of Science  1997

  • Osmotic inhibition of thermally induced cutaneous vasodilation in humans and its role in the inhibition of cutaneous vasodilation during exercise Thermal Physiology 1997

    編著

    The August Krogh Institute, Copenhagen  1997

  • Effect of hydration level on thermoregulatory responses during exercise in rats Thermal Physiology 1997

    編著

    The August Krogh Institute  1997

  • Cardiovascular response to exercise in a hot environment Progress in Biometeology vol. 11 Physiological basis of occupational health: Stressful environment

    編著

    SPB Academic Publishing  1996

▼display all

Misc

  • Surgical masks do not increase the risk of heat stroke during mild exercise in hot and humid environment

    KATO Issei, MASUDA Yuta, NAGASHIMA Kei

    Industrial Health   59 ( 5 ) 325 - 333  2021

     View Summary

    Surgical masks are widely used for the prevention of respiratory infections. However, the risk of heat stroke during intense work or exercise in hot and humid environment is a concern. This study aimed to examine whether wearing a surgical mask increases the risk of heat stroke during mild exercise in such environment. Twelve participants conducted treadmill exercise for 30 min at 6 km/h, with 5% slope, 35°C ambient temperature, and 65% relative humidity, while wearing or not a surgical mask (mask and control trials, respectively). Rectal temperature (Trec), ear canal temperature (Tear), and mean skin temperature (mean Tskin) were assessed. Skin temperature and humidity of the perioral area of the face (Tface and RHface) were also estimated. Thermal sensation and discomfort, sensation of humidity, fatigue, and thirst were rated using the visual analogue scale. Trec, Tear, mean Tskin, and Tface increased during the exercise, without any difference between the two trials. RHface during the exercise was greater in the mask trial. Hot sensation was greater in the mask trial, but no influence on fatigue and thirst was found. These results suggest that wearing a surgical mask does not increase the risk of heat stroke during mild exercise in moist heat.

    DOI CiNii

  • 加湿冷却装置を用いた送風冷却による生理学的変化の検討

    増田 雄太, 加藤 一聖, 丸井 朱里, 永島 計

    日本アスレティックトレーニング学会誌   6   S15 - S15  2021

    DOI CiNii

  • ウェアラブルセンサーを用いた暑熱ストレイン評価手法の実験的検討

    齊藤宏之, 澤田晋一, 赤川宏幸, 笠井泰彰, 飯塚浩二, 山田昇吾, 森川直洋, 永島計

    産業衛生学雑誌   60   326  2018.05

    J-GLOBAL

  • Study on accuracy degradation in high intensity rainfall area observed by X-band MP radar

    Hayashi Yoshiaki, Tebakari Taichi, Nagashima Ken, Hashimoto Akihiro

    Proceeding of Annual Conference   31   136 - 136  2018

     View Summary

    When X-band MP radar has observed high intensity rainfall area, QPE(Quantitative Precipitation Estimation) of X-band MP radar(X-MP) can't often observe with high accuracy. In fact, QPE of X-MP has underestimated against Rain-gauge data and C-band radar data at JMA Asakura by heavy rain in the Northern-Kyushu region on July 2017. So, this study is to clarify observation characteristics of X-MP using this event such as including high intensity rainfall area. This study has used horizontal polarization (Zh) and specific differential phase shift(Kdp) as polarization parameter of X-MP.

    DOI CiNii

  • 血中エストラジオールの消褪は深部体温,心拍数日内リズムの変調をもたらす

    丸井朱里, 内田有希, 永島計

    日本生気象学会雑誌   54 ( 3 ) S80 - S80  2017.10

    J-GLOBAL

  • 送風冷却を併用した短期的暑熱順化トレーニングの効果検証

    増田雄太, 永島計, 坂井禎良, 広瀬統一

    日本生気象学会雑誌   54 ( 3 ) S28 - S28  2017.10

    J-GLOBAL

  • 暑熱負荷時の深部体温の指標としての耳内温の有用性と課題

    齊藤宏之, 澤田晋一, 永島計

    日本生気象学会雑誌   54 ( 3 ) S53 - S53  2017.10

    J-GLOBAL

  • Measuring a woman's temperature during sleep with a new thermometer embedded within the clothes: A case report

    内田有希, 永島計, 永島計, 森本恵子

    早稲田大学人間科学学術院人間科学研究   30 ( 1/Supplement ) 15‐18  2017.03

    J-GLOBAL

  • Measuring a woman's temperature during sleep with a new thermometer embedded within the clothes : A case report

    内田 有希, 永島 計, 森本 恵子

    人間科学研究   30 ( 1 ) 15 - 18  2017

    CiNii

  • 継続した運動トレーニングによる暑熱順化形成メカニズムにおける神経新生の寄与

    綱川みずき, 永島計

    日本生気象学会雑誌   53 ( 3 ) S26 - S26  2016.10

    J-GLOBAL

  • 運動休憩中のファニングによる,その後の疲労感に与える影響の評価

    永島計, 坂井禎良, 広瀬統一

    日本生気象学会雑誌   53 ( 3 ) S71 - S71  2016.10

    J-GLOBAL

  • メスマウスにおける体温調節とハドリングの関連性

    遠藤圭子, 丸井朱里, 加藤一聖, 津谷彰, 永島計

    日本生気象学会雑誌   53 ( 3 ) S70 - S70  2016.10

    J-GLOBAL

  • 雌ラットにおけるエストラジオール欠乏が暗期暑熱時の耐熱能に及ぼす影響

    丸井朱里, 永島計

    日本生気象学会雑誌   53 ( 3 ) S25 - S25  2016.10

    J-GLOBAL

  • Modulation of thermoregulation as an acclimation to cold environment: studies of daily torpor

    永島計, 時澤健

    適応医学   19 ( 2 ) 18‐23 - 23  2016.03

    J-GLOBAL

  • 人の温度感覚と温熱的快適性のメカニズムの探索

    永島計

    温泉療法医会会報   ( 63 ) 5  2016

    J-GLOBAL

  • 温熱感の脳内形成機序の探索

    永島計, 綱川みずき, 原田宗子, 定藤規弘, 中田大貴

    日本感性工学会大会予稿集(CD-ROM)   18th   ROMBUNNO.A54  2016

    J-GLOBAL

  • 高体温障害予防のためのウエアラブルセンサー活用法の探索

    永島計, 坂井禎良, 広瀬統一, 瀬山倫子, 瀬山倫子, 桑原啓, 笠原亮一

    体力科学   64 ( 6 ) 748 - 748  2015.12

    J-GLOBAL

  • 暑熱環境下での間欠的運動時における中間冷却が持久的パフォーマンスに及ぼす影響

    坂井禎良, 永島計, 広瀬統一

    体力科学   64 ( 6 ) 639 - 639  2015.12

    J-GLOBAL

  • 骨格筋SDF‐1α/CXCL12の発現と血管新生

    外薗千紘, 時澤健, 丸井朱里, 宇土泰希, 鈴木克彦, 永島計, 奥津光晴

    体力科学   64 ( 6 ) 568 - 568  2015.12

    J-GLOBAL

  • エストラジオール欠乏が暑熱中の深部体温、尾部皮膚温に及ぼす影響

    丸井 朱里, 永島 計

    日本生気象学会雑誌   52 ( 3 ) S28 - S28  2015.10

  • 長期高脂肪食摂取がマウスの熱産生機能に及ぼす影響

    綱川 みずき, 丸井 朱里, 永島 計

    日本生気象学会雑誌   52 ( 3 ) S30 - S30  2015.10

  • 暑熱暴露時のラットの脳温の部位差と酸化ストレスへの影響

    小幡 千紗, 永島 計

    日本生気象学会雑誌   52 ( 3 ) S35 - S35  2015.10

  • fMRIを用いた温熱的快適性に関わる脳活動の評価

    相澤 優香, 原田 宗子, 綱川 みずき, 定藤 規弘, 永島 計

    日本生気象学会雑誌   52 ( 3 ) S63 - S63  2015.10

  • 環境の生体への影響と適応 寒冷環境の適応としての体温調節反応の変化 日内休眠の研究

    永島 計

    適応医学   19 ( 1 ) 8 - 8  2015.08

  • 運動による骨格筋のSDF‐1α/CXCL12の発現変化

    外薗千紘, 時澤健, 丸井朱里, 鈴木克彦, 永島計, 奥津光晴

    体力科学   64 ( 3 ) 379 - 379  2015.06

    J-GLOBAL

  • 運動トレーニングによる大動脈の抗酸化酵素の発現変化

    奥津光晴, 時澤健, 丸井朱里, 外薗千紘, 鈴木克彦, 永島計

    体力科学   64 ( 3 ) 379 - 380  2015.06

    J-GLOBAL

  • Temporal-Spatial Distribution Characteristics of Quantitative Precipitation Estimation Accuracy using X-band Multi-Parameter Radar over the Hokuriku Region

    HAYASHI Yoshiaki, TEBAKARI Taichi, NAGASHIMA Ken, YAMASAKI Koreyoshi

    JOURNAL OF JAPAN SOCIETY OF HYDROLOGY AND WATER RESOURCES   28 ( 5 ) 221 - 232  2015

     View Summary

    &emsp;This study was designed to clarify the accuracy of X-band multi-parameter radar (X-MP) over the Hokuriku region using rain gauge data and quantitative precipitation estimation (QPE) by C-band radar. Moreover, this study used results of the correlation coefficient and estimate error for QPE accuracy analysis.<br>&emsp;Temporal analysis revealed that X-MPs provide observation data with high accuracy for short-term downpours, but results for long-term and light rainfall or regions distant from the radar site were less accurate.<br>&emsp;Spatial analysis showed the X-MP data accuracy was observable with sufficient accuracy within a range of 2.25 × 2.25 km as the center of each rain gauge station. The X-MP data accuracy has less than the C-band radar data accuracy beyond a 20 km radius from the radar site.<br>&emsp;Quantitative analysis of the rainy season and snowy season, total precipitation of X-MP and C-band radar were overestimated from that of the rain gauge station, it was especially overestimated significantly during the snowy season.

    DOI CiNii

  • STUDY ON CHARACTERISTICS OF OBSERVED POLARIZATION PARAMETER BY X-BAND MULTI-PARAMETER RADAR OVER HOKURIKU REGION

    HAYASHI Yoshiaki, TEBAKARI Taichi, NAGASHIMA Ken, YAMASAKI Koreyoshi

    Journal of Japan Society of Civil Engineers, Ser. B1 (Hydraulic Engineering)   71 ( 4 ) I_475 - I_480  2015

     View Summary

    The purpose of this study is to clarify the characteristics of observed polarization parameter by X-band MP radar (X-MP) over Hokuriku region. Especially, this study analyzed the raindrop size distribution (DSD) using observed horizontal polarization Zh, measured differential reflectivity ZDR and specific differential phase shift KDP as polarization parameter. As a result of analysis of observed polarization parameter, ZDR and Zh have liner relation by heavy rainfall, and ZDR and Zh may be formulated raindrop and snowflake by snowfall and graupel. Precipitation intensity R and ZDR have correlation relation by heavy rainfall, however snowfall haven&rsquo;t that, especially same value of ZDR have widely distributed R by snowfall.

    DOI CiNii

  • 温熱的快適性に関わる脳内機構の解明

    相澤優香, 原田宗子, 中田大貴, 綱川みずき, 定藤規弘, 永島計

    日本感性工学会大会予稿集(CD-ROM)   17th   ROMBUNNO.P37  2015

    J-GLOBAL

  • Modulation of thermoregulation as an acclimation to cold environment : studies of daily torpor

    永島 計, 時澤 健

    適応医学   19 ( 2 ) 18 - 23  2015

    CiNii

  • 環境温と高脂肪高カロリー食が睡眠と体温リズムに及ぼす影響

    佐藤暢夫, 丸井朱里, 永島計

    日本生気象学会雑誌   51 ( 3 ) S62 - S62  2014.10

    J-GLOBAL

  • 暑熱暴露時の脳での酸化,抗酸化反応,褐色脂肪組織の反応から見た熱中症の発生メカニズムの探索と運動による予防効果

    綱川みずき, 丸井朱里, 時澤健, 永島計

    日本生気象学会雑誌   51 ( 3 ) S23 - S23  2014.10

    J-GLOBAL

  • NIRSを用いた温熱的快適感の評価

    相澤優香, 松田真由美, 永島計

    日本生気象学会雑誌   51 ( 3 ) S60 - S60  2014.10

    J-GLOBAL

  • 血中エストラジオール濃度の消褪が雌ラットの尾部皮膚温日内リズムに及ぼす影響

    丸井朱里, 永島計

    日本生気象学会雑誌   51 ( 3 ) S25 - S25  2014.10

    J-GLOBAL

  • 絶食、寒冷暴露における体温リズムと睡眠覚醒リズムの同期性への影響

    佐藤 暢夫, 松田 真由美, 丸井 朱里, 尾崎 眞, 永島 計

    日本睡眠学会定期学術集会プログラム・抄録集   39回   259 - 259  2014.07

  • 暑熱環境と運動 暑熱適応における恒常性の維持と破綻

    永島計

    体育の科学   64 ( 7 ) 456 - 460  2014.07

    J-GLOBAL

  • 暑熱適応における恒常性の維持と破綻 (特集 暑熱環境と運動)

    永島 計

    体育の科学   64 ( 7 ) 456 - 460  2014.07

    CiNii

  • Laboratory of Integrative Physiology : Body temperature and Fluid Laboratory

    永島 計

    人間科学研究   27 ( 1 ) 25 - 26  2014.03

    CiNii

  • 【体温の異常-診かた・考えかた】 ピンポイント小児医療 高体温症、低体温症の病態生理

    永島 計

    小児内科   46 ( 3 ) 310 - 314  2014.03

     View Summary

    <Key Points>(1)恒温動物は、生命活動に必要な高い基礎代謝のために体温の異常が起こった際には、脆弱な動物であるといえる。(2)概日リズム、食事、性周期などによりヒトの体温は変動するが、多くの場合1℃以内にとどまっている。(3)高体温症、低体温症などの体温異常の原因は多くあるが、急性かつ平温からの変動が大きくなるのは暑熱、寒冷などの環境因子が大きい。(4)小児は自律性体温調節が未発達であるが、体温調節異常が起こる原因としてむしろ着衣、エアコンをつけたりする行動性体温調節ができないことが大きい。(5)高体温症と発熱は明確に区別される。(著者抄録)

  • Dehydration attenuates heat sensation: another etiology of heat stroke?

    Nagashima Kei, Lin Chen-Hsien, Tokizawa Ken

      11   48 - 48  2014

    CiNii

  • エストロゲンが与えるメスマウスの温度嗜好性

    綱川みずき, 小幡千紗, 原田大義, 松下はるか, 脇直也, 丸井朱里, 永島計

    日本生気象学会雑誌   50 ( 3 ) S87 - S87  2013.10

    CiNii J-GLOBAL

  • 雌ラットにおけるエストロゲンの寒冷時体温調節行動への影響

    内田有希, 大迫洋治, 山口奈緒子, 永島計, 由利和也

    日本生気象学会雑誌   50 ( 3 ) S59 - S59  2013.10

    CiNii J-GLOBAL

  • NIRSを用いた温度感覚の評価

    相澤優香, 丸井朱里, 松田真由美, 永島計

    日本生気象学会雑誌   50 ( 3 ) S33 - S33  2013.10

    CiNii J-GLOBAL

  • 頭頸部冷却が深部体温に及ぼす影響

    松田真由美, 和田哲志, 丸井朱里, 佐藤暢夫, 永島計

    日本生気象学会雑誌   50 ( 3 ) S61 - S61  2013.10

    CiNii J-GLOBAL

  • マウス非活動期に見られる体温のウルトラディアンリズムは睡眠覚醒リズムに追随する

    佐藤暢夫, 丸井朱里, 松田真由美, 永島計

    日本生気象学会雑誌   50 ( 3 ) S26 - S26  2013.10

    CiNii J-GLOBAL

  • エストロゲンが雌ラットの心拍,動脈圧日内リズムに及ぼす影響

    丸井朱里, 綱川みずき, 小幡千紗, 松下はるか, 松田真由美, 永島計

    日本生気象学会雑誌   50 ( 3 ) S23 - S23  2013.10

    J-GLOBAL

  • エストログンが雌ラットの心拍、動脈圧日内リズムに及ぼす影響

    丸井 朱里, 綱川 みずき, 小幡 千紗, 松下 はるか, 松田 真由美, 永島 計

    日本生気象学会雑誌   50 ( 3 ) S23  2013.10

    CiNii

  • 環境ストレス時(絶食,温熱負荷)の生物時計による体温調節修飾メカニズムの解析

    永島計, 時澤健, 内田有希, 中村真由美, 依田珠江

    早稲田大学人間科学学術院人間科学研究   26 ( 2 ) 213 - 214  2013.09

    J-GLOBAL

  • 環境ストレス時 (絶食、温熱負荷) の生物時計による体温調節修飾メカニズムの解析

    永島 計, 時澤 健, 内田 有希, 中村 真由美, 依田 珠江

    人間科学研究   26 ( 2 ) 213 - 214  2013.09

    CiNii

  • Physiological and psychological responses to color lights under cold water immersion test

    GUO Yang, 齋藤美穂, 男座慶一, 時澤健, 永島計

    日本色彩学会誌   37 ( 1 ) 15 - 26  2013.01

    J-GLOBAL

  • Physiological and psychological responses to color lights under cold water immersion test

    GUO Yang, SAITO Miho, OZA Keiichi, TOKIZAWA Ken, NAGASHIMA Kei

    日本色彩学会誌   37 ( 1 ) 15 - 26  2013.01

     View Summary

    The purpose of this study was to examine the physiological and psychological responses to color lights during cold water immersion test. Ten female subjects aged between 20 to 30 years were asked to immerse their left hand in 15℃ water for 3 minutes respectively under white, blue and red color light. The subjects&#039; skin temperature, skin blood flow, blood pressure and electrocardiogram were continuously measured. At the meantime, psychological questionnaires were conducted, focusing on subjects&#039; impression of the color lights, mood, thermesthesia, cold discomfort, and subjective feelings of temperature recovery during the test. The physiological results indicate that under blue color light, reduced skin temperature under cold water immersion recovered relatively faster compared to other color lights. Skin blood flow also recovered faster under blue color light. The psychological results show difference in the impression among 3 color lights in terms of &quot;pleasant&quot; and &quot;warm and cool&quot;. Meanwhile, subjects&#039; mood and thermesthesia were different to each color lights. Specifically, blue color light, evaluated as cool color was considered increasing the discomfort of cold, thus leading to emotional arousal. On the other hand, red color light, evaluated as warm color was considered decreasing the discomfort of cold. In this study, as the characteristic of blue color light, emotional arousal was estimated associated with skin sympathetic nerve inhibition. This study may suggest color lights affect mood, thermesthesia and thermoregulation through cognitive process.

    CiNii

  • 性差の運動生理学 体温と性差

    永島計, 中村真由美

    体育の科学   62 ( 12 ) 928 - 933  2012.12

    J-GLOBAL

  • 体温と性差 (特集 性差の運動生理学)

    永島 計, 中村 真由美

    体育の科学   62 ( 12 ) 928 - 933  2012.12

    CiNii

  • 生体リズムと自律神経障害 体温のリズム形成と摂食、睡眠との関係

    永島 計, 時澤 健, 佐藤 暢夫

    日本自律神経学会総会プログラム・抄録集   65回   61 - 61  2012.10

  • 女性ホルモンのエストロゲンが雌ラットの生理機能に与える影響

    丸井朱里, 松田真由美, 永島計

    日本生気象学会雑誌   49 ( 3 ) S55 - S55  2012.10

    CiNii J-GLOBAL

  • Ghrelin, but not leptin depletion, induces a circadian nadir of thermoregulation in the cold : a possible neurophysiological mechanism of torpor-like hypothermia during fasting

    NAGASHIMA Kei

    自律神経 = The Autonomic nervous system   49 ( 2 ) 67 - 69  2012.06

    CiNii

  • ひろがりを見せる概日リズム研究の新展開 絶食時に見られる体温調節リズムの変調と摂食ペプチドのかかわり 摂食情報受容部としての視交叉上核の役割

    永島 計

    自律神経   49 ( 2 ) 67 - 69  2012.06

  • ヒトにおける体温の意味と意義

    永島計, 中村(松田)真由美

    早稲田大学人間科学学術院人間科学研究   25 ( 1/Supplement ) 21 - 33  2012.03

    CiNii J-GLOBAL

  • 【周術期の低体温】 呼吸・循環への低体温の影響 体温の低下に対して変化は一様ではない 医療の介入や薬物による反応の増強に注意

    永島 計, 時澤 健, 内田 有希

    LiSA   19 ( 1 ) 14 - 17  2012.01

     View Summary

    低体温は,広い意味での呼吸(肺胞から末梢組織でのガス交換)・循環に大きな影響を及ぼすが,その影響は,低体温に陥った状況によりさまざまである。すなわち,救急外来に運ばれてきた低体温でも,事故や疾患の種類(寒冷環境への長期曝露や脳疾患,意識消失などに伴う体温調節反応の減弱など)によって異なるし,病院の中でも,病棟で起こったか,手術室で起こったかで大きく異なる。また近年,低体温療法が脳や心筋障害時の臓器保護の方法として用いられるようになり,低体温が人為的に行われる場合もある。病棟や手術室においても,補液や循環管理がなされているか,気道が確保されているか,さらには人工呼吸器でコントロールされているか,麻酔薬や筋弛緩薬,カテコールアミンが用いられているか,などによって呼吸・循環への影響は大きく異なる。もちろん,肺や呼吸中枢,心臓・血管にかかわる基礎疾患の有無にも左右される。本稿では,以上のような個別の状況に応じて低体温の影響を評価するために必要になる,基礎的な低体温時の呼吸・循環の変化について解説する。(著者抄録)

  • 体温のリズム形成と摂食,睡眠との関係

    永島計, 時澤健, 佐藤暢夫

    日本自律神経学会総会プログラム・抄録集   65th   61  2012

    J-GLOBAL

  • 暑熱下のマウス自発運動による暑熱耐性の獲得と視床下部神経新生

    時澤健, 林政賢, 内田有希, 榊原伸一, 永島計

    体力科学   60 ( 6 ) 739 - 739  2011.12

    J-GLOBAL

  • Thermoregulation in humans : What is regulated and why?

    Kanosue Kazuyuki, Crawshaw Larry, Yoda Tamae, Nakamura Mayumi, Nagashima Kei

    Proceedings of the ... Symposium on Human-Environment System   2011   4 - 4  2011.10

    CiNii

  • ひろがりを見せる概日リズム研究の新展開 絶食時に見られる体温調節リズムの変調と摂食ペプチドのかかわり 摂食情報受容部としての視交叉上核の役割

    永島 計

    日本自律神経学会総会プログラム・抄録集   64回   44 - 44  2011.10

  • カプサイシン/メントール混和蒸気シートによる‘こり’の改善効果

    張つぼみ, 田上恭子, 坂本一郎, 井垣通人, 永島計

    日本生気象学会雑誌   48 ( 3 ) S71 - S71  2011.09

    CiNii J-GLOBAL

  • 摂食が睡眠/覚醒,生理リズムに与える影響とその神経メカニズムの解析

    森久恵, 永島計

    日本生気象学会雑誌   48 ( 3 ) S40 - S40  2011.09

    CiNii J-GLOBAL

  • 体温調節系の日内変動解析

    時澤健, 依田珠江, 内田有希, 中村真由美, 彼末一之, 永島計

    時間生物学   16 ( 2 ) 78  2010.10

    J-GLOBAL

  • 大学生の体温と生活習慣~低体温に悩む若年者は本当に増えているのか?~

    永島計, 丸井朱里, 田中祐輝, 三澤彩佳

    日本生気象学会雑誌   47 ( 3 ) S84 - S84  2010.09

    CiNii J-GLOBAL

  • 概日リズムおよび絶食が環境温・深部体温の関係に及ぼす影響

    時澤健, 依田珠江, 内田有希, 中村真由美, 彼末一之, 永島計

    日本生気象学会雑誌   47 ( 3 ) S80 - S80  2010.09

    CiNii J-GLOBAL

  • グレリンは時間特異的に寒冷時の体温調節に影響を与える(Ghrelin plays an important role in time-dependent change in thermoregulation during cold in mice)

    時澤 健, 尾上 侑己, 内田 有希, 森 久恵, 中村 真由美, 永島 計

    神経化学   49 ( 2-3 ) 651 - 651  2010.08

  • Evaluation of new thermoregulatory behavior

    内田 有希, 時澤 健, 中村 真由美, 森 久恵, 永島 計

    人間科学研究   23 ( 1 ) 135 - 135  2010.03

    CiNii

  • Role of leptin and ghrelin on circadian rhythm of body temperature

    時澤 健, 尾上 侑己, 内田 有希, 森 久恵, 中村 真由美, 永島 計

    人間科学研究   23 ( 1 ) 136 - 136  2010.03

    CiNii

  • 生物時計による体温・代謝調節制御メカニズムの全容解明

    永島 計, 時澤 健, 尾上 侑己, 内田 有希, 森久 恵, 中村 真由美

    人間科学研究   23 ( 1 ) 168 - 169  2010.03

    CiNii

  • ライフステージに応じた健康増進に関する健康・生命科学的研究

    鈴木 秀次, 木村 一郎, 小室 輝昌, 今泉 和彦, 山内 兄人, 永島 計, 竹中 晃二, 辻内 琢也, 鈴木 克彦

    人間科学研究   23 ( 1 ) 152 - 153  2010.03

    CiNii

  • 色彩の冷え性女性に対する生理的・心理的効果の研究 -五行思想に基づく色光による冷えの改善を目指して-

    永島 計, 郭 洋, 中村 真由美, 内田 有希, 時澤 健, 齋藤 美穂

    人間科学研究   23 ( 1 ) 174 - 175  2010.03

    CiNii

  • 卵白ペプチドが心肺機能及び持久性運動に及ぼす影響

    坂下真耶, 佐内勇亮, 青木恵理, 中村真由美, 天野剛, 永島計

    日本運動生理学会大会プログラム・抄録集   18th   79  2010

    J-GLOBAL

  • レプチンが寒冷時体温調節反応および絶食時日内休眠に及ぼす影響

    時澤健, 永島計

    体力科学   58 ( 6 ) 854 - 854  2009.12

    J-GLOBAL

  • 温度感覚,温熱的快適感の部位差

    中村真由美, 依田珠江, CRAWSHAW Larry I, 安原祥, 齋藤恭世, 春日桃子, 永島計, 彼末一之

    日本生気象学会雑誌   46 ( 3 ) S69 - S69  2009.09

    CiNii J-GLOBAL

  • 雄ラットにおいて尾隠し行動が寒冷時の体温に与える影響

    内田有希, 時澤健, 中村真由美, 永島計

    日本生気象学会雑誌   46 ( 3 ) S13  2009.09

    J-GLOBAL

  • 雄ラットにおいて尾隠し行動が寒冷時の体温に与える影響

    内田 有希, 時澤 健, 中村 真由美, 永島 計

    日本生気象学会雑誌   46 ( 3 ) S13  2009.09

    CiNii

  • 脳内エストロゲン投与による寒冷時体温調節反応への影響

    内田有希, 時澤健, 永島計

    早稲田大学人間科学学術院人間科学研究   22 ( 1 ) 53  2009.05

    J-GLOBAL

  • 温度感覚,温熱的快適感の部位差

    中村真由美, 依田珠江, 内田有希, 時澤健, 永島計, 彼末一之

    早稲田大学人間科学学術院人間科学研究   22 ( 1 ) 69  2009.05

    J-GLOBAL

  • Clock変異マウスを用いた,絶食時の体温リズム変調メカニズムの解析

    時澤健, 内田有希, 永島計

    早稲田大学人間科学学術院人間科学研究   22 ( 1 ) 56  2009.05

    J-GLOBAL

  • Effect of Hypothalamic Administration of 17-β Estradiol on Thermoregulatory Responses during Cold Exposure in Ovariectomized Rats

    内田 有希, 時澤 健, 永島 計

    人間科学研究   22 ( 1 ) 53 - 53  2009.05

    CiNii

  • Effect of Fasting on Circadian Body Temperature Rhythm in Clock Mutant Mice

    時澤 健, 内田 有希, 永島 計

    人間科学研究   22 ( 1 ) 56 - 56  2009.05

    CiNii

  • 色光が手掌冷水負荷における体温調節反応および気分に与える影響

    郭洋, 齋藤美穂, 男座慶一, 時澤健, 永島計

    日本色彩学会誌   33 ( Supplement ) 46 - 47  2009.05

    J-GLOBAL

  • The effects of color light on the human thermoregulation and mood under the condition of cold water load

    KAKU You, SAITO Miho, OZA Keiichi, TOKIZAWA Ken, NAGASHIMA Kei

    Journal of the color science association of Japan   33   46 - 47  2009.05

    CiNii

  • ライフステージに応じた健康増進に関する健康・生命医科学的研究

    鈴木 秀次, 木村 一郎, 小室 輝昌, 今泉 和彦, 山内 兄人, 竹中 晃二, 永島 計, 辻内 琢也, 鈴木 克彦

    人間科学研究   22 ( 0 ) 136 - 136  2009.03

    CiNii

  • 色彩の冷え性女性に対する生理的・心理的効果の研究 : 五行思想に基づく色光による冷えの改善を目指して

    永島 計, 郭 洋, 時澤 健, 齋藤 美穂

    人間科学研究   22 ( 0 ) 148 - 148  2009.03

    CiNii

  • 生物時計による体温・代謝調節反応制御メカニズムの全容解明 : Clock変異マウスを用いた、絶食時の体温リズム変調メカニズムの解析

    永島 計, 郭 洋, 時澤 健

    人間科学研究   22 ( 0 ) 145 - 145  2009.03

    CiNii

  • レプチンおよびグレリンが絶食時の時間特異的体温調節反応に及ぼす影響

    時澤健, 尾上侑己, 内田有希, 森久恵, 中村真由美, 永島計

    Program Abstr 6th Congr Asian Sleep Res Soc 34th Annu Meet Jpn Soc Sleep Res 16th Annu Meet Jpn Soc Chronobiol Jt Congr 2009     136  2009

    J-GLOBAL

  • 絶食時の体温概日リズム調節機構の解明

    時澤健, 内田有希, 永島計

    時間生物学   14 ( 2 ) 61  2008.10

    J-GLOBAL

  • カプシノイドによる消化管迷走神経を介した褐色脂肪組織特異的な交感神経活動の亢進

    安居昌子, 小野郁, 原佳子, 井上尚彦, 野草義人, 岡辺有紀, 永島計, 加藤総夫

    肥満研究   14 ( Supplement ) 260 - 260  2008.09

    J-GLOBAL

  • 暑熱下運動による軽度脱水が温熱的感覚に及ぼす影響

    時澤健, 永島計

    日本運動生理学会大会プログラム・抄録集   16th   72  2008

    J-GLOBAL

  • The regional characteristics of temperature-related sensations.

    Nakamura Mayumi, Kasuga Momoko, Yoda Tamae, saito yasuyo, yasuhara saki, nagashima kei, Crawshaw Larry, kanosue kazuyuki

    Proc Annu Meet PSJ   2008 ( 0 ) 102 - 102  2008

     View Summary

    We examined the regional sensitivity in temperature sensation and thermal comfort/discomfort. Subjects sitting in a 33&amp;deg;C environment were locally cooled and warmed with water perfused stimulators (270cm&lt;SUP&gt;2&lt;/SUP&gt;). The water for basal, cooling, and warming conditions was set at 35&amp;deg;C, 25&amp;deg;C, and 42&amp;deg;C respectively. The areas stimulated were the neck, abdomen, hand and sole. Each stimulus lasted 90 s. Temperature sensation and thermal comfort/discomfort of stimulated area and those of whole body were reported by the subjects. At the basal condition just before the local stimulation of each area subjects reported hot and uncomfortable for whole body sensations. At the end of 90 s cooling, subjects reported clear cold sensation for local temperature sensation and no significant difference was observed among those of the four areas. But for local thermal comfort/discomfort at the same time point, depended on the area stimulated. While abdominal cooling produced no pleasant feeling, cooling of the other parts produced clear pleasant sensation. As for local warming, at the end of 90 s stimulation subjects reported hot and uncomfortable sensation and no significant difference was observed among those of the four areas. These results show that humans tend to keep the abdomen warm even during whole body heat exposure. &lt;b&gt;[J Physiol Sci. 2008;58 Suppl:S102]&lt;/b&gt;

    DOI CiNii

  • Difference in thermoregulation between dark and light phase of fasted mice

    Tokizawa Ken, Uchida Yuki, Nagashima kei

    Proc Annu Meet PSJ   2008 ( 0 ) 87 - 87  2008

     View Summary

    We have shown that circadian body temperature (T&lt;SUB&gt;b&lt;/SUB&gt;) rhythm is finely regulated, but feeding condition affects largely the T&lt;SUB&gt;b&lt;/SUB&gt; rhythm. During fasting, T&lt;SUB&gt;b&lt;/SUB&gt; gradually decreases in the light (inactive) phase, whereas T&lt;SUB&gt;b&lt;/SUB&gt; in the dark (active) phase is maintained at the free-feeding level. The purpose of this study was to elucidate the mechanism involved in the altered thermoregulation between dark and light phase during fasting. Male ICR mice (2-3 mo old) were housed at an ambient temperature (T&lt;SUB&gt;a&lt;/SUB&gt;) of 27&amp;deg;C in a 12:12-h light-dark cycle. After 48-h fasting, cold exposure (T&lt;SUB&gt;a&lt;/SUB&gt;=20&amp;deg;C) was carried out at light phase (ZT1-4) or dark phase (ZT13-16). T&lt;SUB&gt;b&lt;/SUB&gt; (biotelemetry) and oxygen consumption (VO&lt;SUB&gt;2&lt;/SUB&gt;, indirect calorimetry) were measured. c-Fos immunostaining was also performed after the cold exposure. During the cold exposure with fasting, T&lt;SUB&gt;b&lt;/SUB&gt; decreased by 5.5 &amp;plusmn; 2.0&amp;deg;C at light phase, and VO&lt;SUB&gt;2&lt;/SUB&gt; remained unchanged. In contrast, at dark phase, T&lt;SUB&gt;b&lt;/SUB&gt; decreased by only 2.0 &amp;plusmn; 0.5&amp;deg;C with an increase in VO&lt;SUB&gt;2&lt;/SUB&gt; (p&lt;0.05), which was greater than that in the light phase (p&lt;0.05). The number of c-Fos-immunoreactive cells after the cold exposure with fasting were greater at the dark phase than the light phase in the medial preoptic area, dorsomedial hypothalamus, paraventricular hypothalamic nucleus, and arcuate nucleus. These results indicate that fasting attenuates thermoregulatory response to the cold, and the response differs among time of the day. The difference between the dark and light phase would be partially due to the neuronal activity in the hypothalamus. &lt;b&gt;[J Physiol Sci. 2008;58 Suppl:S87]&lt;/b&gt;

    DOI CiNii

  • Effect of hypothalamic application of 17-&beta;estradiol on thermoregulation during a cold exposure in female rats

    Uchida Yuki, Tokizawa Ken, Nagashima Kei

    Proc Annu Meet PSJ   2008 ( 0 ) 100 - 100  2008

     View Summary

    &lt;B&gt;INTRODUCTION&lt;/B&gt; We reported that, in ovariectomized rats, thermoregulation in the cold was attenuated. Systemic administration of 17-&amp;beta;estradiol (E&lt;SUB&gt;2&lt;/SUB&gt;) restored the attenuated response. E&lt;SUB&gt;2&lt;/SUB&gt; increased &lt;I&gt;cFOS&lt;/I&gt; immunoreactive cells in the medial preoptic area ( MPO ) and dorsomedial hypothalamus ( DMH ) in the cold. In the present study, we hypothesized that E&lt;SUB&gt;2&lt;/SUB&gt; would upregulate thermosensitivity to the cold at the level of the hypothalamus. To test this hypothesis, we assessed the effect of the local administration of E&lt;SUB&gt;2&lt;/SUB&gt; to the two hypothalamic areas on body temperature ( T&lt;SUB&gt;b&lt;/SUB&gt; ) in the cold. &lt;B&gt;METHODS&lt;/B&gt; Adult female rats were ovariectomized, and a stainless steel canula was placed in the MPO, DMH, or horizontal limb diagonal band. At least 7 days after the surgery, E&lt;SUB&gt;2&lt;/SUB&gt; or cholesterol was administrated to this brain area through the canula. Forty-eight hours after the administration, a rat was exposed to 10&amp;deg;C or 25&amp;deg;C environment for 2h, and T&lt;SUB&gt;b&lt;/SUB&gt; was continuously measured. &lt;B&gt;RESULTS&lt;/B&gt; During the 10&amp;deg;C exposure, T&lt;SUB&gt;b&lt;/SUB&gt; increased only in the group, which is administrated E&lt;SUB&gt;2&lt;/SUB&gt; in the MPO. Either cholesterol nor E&lt;SUB&gt;2&lt;/SUB&gt; in the other brain area had no effect on T&lt;SUB&gt;b&lt;/SUB&gt;. &lt;B&gt;CONCLUSION&lt;/B&gt; The MPO has abundant estrogen receptors and so-called thermosensitive neurons, estrogen may modulate thermosensitivity to the cold in the MPO. We also clarified the mechanism, based on &lt;I&gt;cFOS&lt;/I&gt; immunoreactivity in the hypothalamic areas and &lt;I&gt;UCP1&lt;/I&gt; mRNA expression in the interscapular brown adipose tissue. &lt;b&gt;[J Physiol Sci. 2008;58 Suppl:S100]&lt;/b&gt;

    DOI CiNii

  • Estrus cycle modulates thermoregulatory responses to the cold in rats

    Nagashima Kei, Kano Masumi, Uchida Yuki, Tokizawa Ken

    Proc Annu Meet PSJ   2008 ( 0 ) 60 - 60  2008

     View Summary

    &lt;B&gt;Introduction&lt;/B&gt; We have reported that estrogen has influence on thermoregulation in the heat and cold. In ovariectomized rats, in the heat, an increase in body temperature (T&lt;SUB&gt;b&lt;/SUB&gt;) was greater with a reduction in heat dissipation from the skin. In the cold, T&lt;sub&gt;b&lt;/sub&gt; was smaller with a decrease in metabolic heat production. However, 17&amp;beta;-estradiol (E&lt;SUB&gt;2&lt;/SUB&gt;) replacement restores these responses to normal levels. The purpose of the present study was to test the hypothesis that the estrogen fluctuation in normal estrus cycle would modulate the thermoregulary response to the cold in rats. &lt;B&gt;Methods&lt;/B&gt; Female WKY rats were laparectomized and radiotelemeters for T&lt;sub&gt;b&lt;/sub&gt; were placed. At least 2 wks after the surgery, rats&#039; vaginal smear was obtained to determine the estrus cycle. At 9 am, rats in proestrus or diestrus period were exposed to 5&amp;deg;C or 25&amp;deg;C environment for 2 h, and T&lt;SUB&gt;b&lt;/SUB&gt; was monitored. After the cold exposure, rats were perfused with phosphate buffered saline and the brains were excised. cFos immunoreactive cells in the hypothalamic area were counted. &lt;B&gt;Results&lt;/B&gt; T&lt;SUB&gt;b&lt;/SUB&gt; increased in the cold in the proestrus period, but decreased in the diestrus period. Plasma E&lt;SUB&gt;2&lt;/SUB&gt; was higher in the proestrus period than estrus period with a small difference in progesterone level. cFos immunoreactive cells in the medial preoptic area and dorsomedial hypothalamus were greater in the proestrus period. &lt;B&gt;Conclusion&lt;/B&gt; Thermoregulation in the cold differs among the periods of estrus cycle in rats. The fluctuation of plasma E&lt;SUB&gt;2&lt;/SUB&gt; level may be involved in the mechanism. &lt;b&gt;[J Physiol Sci. 2008;58 Suppl:S60]&lt;/b&gt;

    DOI CiNii

  • 性ホルモンによる体温調節反応の修飾

    永島計, 時澤健, 狩野真清, 内田有希, 安原祥

    Biomed Thermology   27 ( 1 ) 39 - 39  2007.08

    CiNii J-GLOBAL

  • Neural mechanisms involved in thermoregulation regulation and their importance in physiology and human health

    永島 計

    人間科学研究 = Waseda Journal of Human Sciences   20 ( 0 ) 200 - 200  2007.03

    CiNii

  • 「女性ホルモンが体温調節反応に与える影響:自律神経調節から温度感覚まで」

    永島計, 時澤健, 狩野真清, 安原祥, 内田有希

    快適性を考えるシンポジウム   34th   17 - 20  2007

    J-GLOBAL

  • The regional difference in temperature sensation and thermal comfort/discomfort in human

    Nakamura Mayumi, Yoda Tamae, Yasuhara Saki, Saito Yasuyo, Nagashima Kei, Crawshaw Larry I, Kanosue Kazuyuki

    Proc Annu Meet PSJ   2007 ( 0 ) 184 - 184  2007

     View Summary

    We examined the regional difference in temperature sensation and thermal comfort/discomfort. Subjects sitting in a 32-33&amp;deg;C environment were locally cooled and warmed with water perfused stimulators. The areas stimulated were the face, chest, abdmen and thigh. The temperature of water for cooling was 25&amp;deg;C, and that for warming was 42&amp;deg;C. The area of stimulation was 270 cm&lt;SUP&gt;2&lt;/SUP&gt; and each stimulation lasted 1.5 min. Temperature sensation and thermal comfort/discomfort of stimulated area and those of whole body were reported by the subjects using dials numbered from -10 to 10 (0 : neutral). The setting of the dial procedured a voltage which was continuously recorded. At the basal condition without local stimulation subjects reported hot and uncomfortable for whole body sensation. During local thermal stimulation there is no significant difference in local temperature sensation among the four areas. But for thermal comfort/discomfort, face and abdmen displayed characteristic sensation for cold stimulation. Thermal comfort was strongest for the face by cooling. On the other hand, abdominal cooling produced discomfort even in the hot environment. These results suggest that there is regional difference in the production of thermal comfort/discomfort. &lt;b&gt;[J Physiol Sci. 2007;57 Suppl:S184]&lt;/b&gt;

    DOI CiNii

  • Influence of feeding on thermoregulation

    Nagashima Kei

    Proc Annu Meet PSJ   2007 ( 0 ) 32 - 32  2007

     View Summary

    &lt;B&gt;Introduction&lt;/B&gt; Body temperature is finely regulated, although feeding condition is a factor modulating the regulation. Fasting is a strong stimulus reducing metabolism (i.e. heat production). However, body temperature is well maintained in the active phase, indicating change in thermoregulation. I discuss about neural and hormonal mechanisms involved in the change in thermal regulation during fasting and the possible role of clock genes. &lt;B&gt;Experiments and Results&lt;/B&gt; 1) Fasting induced greater reduction of metabolism and its circadian amplitude. Although the reduction of heat production, body temperature rhythm was well maintained except for greater decrease of body temperature around the lights onset. During the fasting, body surface temperature was greatly reduced, meaning that heat loss mechanisms were suppressed. 2) During fasting, heat producing response to the cold was attenuated in rats. However, this response is attenuated in the presence of i.c.v. leptin. 3) Feed-fast cycle generated daily oscillation of body temperature in clock mutant and cry knockout mice, both mice could not modulate heat loss responses to the fasting. 4) Fasting affects neural activities in the suprachiasmatic nucleus, estimated by c-Fos expression. &lt;B&gt;Conclusion&lt;/B&gt; Fasting is a strong signal modulating thermal regulation. Leptin level in the central may be a factor to change metabolic activity, deactivating the sympathetic nerves to the brown adipose tissue. Moreover, circadian clock receive fasting signals, which may alter heat loss responses and maintain body temperature rhythm. &lt;b&gt;[J Physiol Sci. 2007;57 Suppl:S32]&lt;/b&gt;

    DOI CiNii

  • 入浴後の深部体温の変化が生体内酸化ストレス(尿中8‐0HdG排泄量)に及ぼす影響

    齋藤恭世, 齋藤武比斗, 永島計, 須田和裕, 彼末一之, 田中英登

    人間-生活環境系シンポジウム報告集   30th   233 - 236  2006.12

    J-GLOBAL

  • 生理学者であること?

    永島 計

    日本生理学雜誌 = JOURNAL OF THE PHYSIOLOGICAL SOCIETY OF JAPAN   68 ( 12 ) 450 - 451  2006.12

    CiNii

  • 体温調節,温冷感覚にあたえる性周期の影響

    安原祥, 岩間愛音, 狩野真清, 永島計

    日本生気象学会雑誌   43 ( 3 ) S41 - S41  2006.10

    CiNii J-GLOBAL

  • ヒトにおける一過性運動時の環境温度の違いがその後の生体内酸化ストレスに及ぼす影響

    齋藤恭世, 永島計, 彼末一之, 田中英登

    日本生気象学会雑誌   43 ( 3 ) S43 - S43  2006.10

    CiNii J-GLOBAL

  • 体温調節反応に対するエストロゲンの効果

    狩野真清, 安原祥, 永島計

    日本生気象学会雑誌   43 ( 3 ) S42 - S42  2006.10

    CiNii J-GLOBAL

  • メスラットの体温調節反応に対するエストロゲンの役割

    永島計, 狩野真澄, 安原祥

    日本内分泌学会雑誌   82 ( 2 ) 367 - 367  2006.09

    J-GLOBAL

  • 蒸気温熱シートによる腰部加温が体温調節反応と感覚に及ぼす効果(Effects of warming the lower back with a heat and steam generating sheet on thermoregulatory responses and sensation)

    小田 英志, 井垣 通人, 宇賀神 徹, 鈴木 敦, 土屋 秀一, 永島 計, 礒 繁雄, 彼末 一之

    日本生気象学会雑誌   43 ( 1 ) 43 - 50  2006.05

     View Summary

    著者らは長時間水蒸気を発生する蒸気温熱(HSG)シートを作製した.これを水蒸気を発生しない乾熱(HG)シートと比較し,その体温調節反応および感覚に及ぼす効果について検討した.その結果, 1)シート適用部直下の皮膚温度はHSGシートとHGシートで同等であったにもかかわらず,シートから外側2cmの皮膚温度はHSGシートでおよそ0.9℃高かった. 2)被験者はHSGシートを適用したとき,HGシートに比べて体全体の冷え感覚が有意に抑制され,加温部の温感がより温かくより広範囲に広がる感覚が得られた. 3)熱流束測定装置を用いた別個の試験では,HSGシートとHGシートは同じ表面温度であったにもかかわらず,HSGシートの熱流束はHGシートの熱流束よりも高かった.以上,これらのことからも,蒸気で皮膚を加温することは乾いた熱で加温することに比べてより効果的であることが示唆された

    DOI

  • Effects of alcohol on thermoregulation in humans

    Yoda Tamae, Saito Kumiko, Nakamura Mayumi, Crawshaw Larry, Nagashima Kei, Kanosue Kazuyuki

    Proc Annu Meet PSJ   2006 ( 0 ) 229 - 229  2006

     View Summary

    We investigated the effects of alcohol on thermoregulatory responses and thermal sensations during cold exposure in humans. Eight healthy men participated in this study. Experiments were conducted twice for each subject at a room temperature of 18 &amp;deg;C. After a 30-min resting period, the subject drank either 15% alcohol (alcohol session) at a dose of 0.36 g/kg body weight or an equal volume of water (control session). Deep body temperature gradually decreased throughout 90-min measurement both in the alcohol and control sessions (from 36.9 &amp;plusmn; 0.1 &amp;deg;C to 36.6 &amp;plusmn; 0.1 &amp;deg;C) without any statistically significant differences. Metabolic rate in the control session started to increase 30 min after the onset of measurement. On the other hand, in the alcohol session, metabolic rate remained unchanged in spite of a decrease in body core temperature. Whole body cold sensation became strong in the control session during cold exposure, whereas it changed to &quot;not cold at all&quot; after alcohol drinking, which would inhibit the behavioral regulation, if available. In the previous study we have already shown that both autonomic and behavioral thermoregulation is also modulated to decrease body temperature in hot environment (Yoda et al., 2005). Thus, alcohol influences all thermoregulatory mechanisms including behavior so as to decrease body core temperature. These results suggest that alcohol affects some elements common to all the effector mechanisms, most presumably thermosensitive neurons in the brain. &lt;b&gt;[J Physiol Sci. 2006;56 Suppl:S229]&lt;/b&gt;

    DOI CiNii

  • Central control of thermoregulatory vasomotor response to hypertonic stimulation in rats

    Kobayashi Akiko, Nagashima Kei

    Proc Annu Meet PSJ   2006 ( 0 ) 231 - 231  2006

     View Summary

    Heat loss responses are suppressed during dehydration, and hyperosmolality in the extracellular fluid is thought to be involved in this mechanism. In an &lt;I&gt;in-vitro&lt;/I&gt; brain slice, warm-sensitive neurons in the medial preoptic area (MPA) are deactivated in a hyperosmotic medium. In contrast, neural activities in the median preoptic nucleus (MnPO) increase during both heat and osmotic stimuli. In the present study, we hypothesized that tail vasodilatation induced by local warming of the MPA in urethane-anesthetized rats would be suppressed by a selective infusion of hypertonic saline to the brain. In addition, rats with the MnPO lesion would lack this suppression. The MPA warming at &amp;sim;40 &amp;deg;C increased skin temperature at the tail. Hypertonic saline (1500 mM) infusion into the left internal carotid artery suppressed this response when the MPA was warmed in its dorsolateral area. In MnPO-lesioned rats, there was no effect of hypertonic-saline infusion on the tail skin temperature during the MPA warming. These results indicate that the tail vasodilatation elicited by MPA warming is suppressed during osmotic stimulus. Moreover, the dorsolateral area in the MPA is a crucial site for such a response. The MnPO is also involved in this mechanism. &lt;b&gt;[J Physiol Sci. 2006;56 Suppl:S231]&lt;/b&gt;

    DOI CiNii

  • A new simple apparatus for the study of rodent behavioral thermoregulation using a principle of alternative selection of ambient temperatures

    Takayoshi Hosono, Kanosue Kazuyuki, Nagashima Kei

    Proc Annu Meet PSJ   2006 ( 0 ) 227 - 227  2006

     View Summary

    Recent instruments for the study of thermoregulation in rodents are usually large and often require troublesome trainings of animals. In this study, we designed, made and tested a new simple instrument for investigating rodents&#039; behavioral thermoregulation. The apparatus was composed of two stainless-steel hollow plates (plate A and plate B) with a length of 20cm and width of 5cm. Each plate had an inlet and an outlet that were connected to a separate constant-temperature bath (bath 1 and bath 2). The water temperatures of the baths were controlled at one designated temperature within 10 and 45 degrees and pumped into the plates. A change switch for the water supply was inserted between both the plates and the baths. In the normal switch position, bath 1 supplied water plate A, and bath 2 supplied water plate 2, and in the reverse position, vice versa. Plates A and B were arranged and covered with a surrounding transparent fence 20cm high in a climatic chamber. A rodent stayed inside the fence and moved on plate A or plate B. The position of the rodent was observed by a video camera. We tested thermoregulatory behavior of eight male mice using this instrument. By shuffling the plate temperatures between 10 and 45 degree, the mice moved to the plates with a temperature close to the 35 degree. The findings implied our instrument might be useful as an apparatus for the study of behavioral thermoregulation. &lt;b&gt;[J Physiol Sci. 2006;56 Suppl:S227]&lt;/b&gt;

    DOI CiNii

  • Sex difference in thermoregulation-impact of estrogen on thermoregulation-

    Nagashima Kei, Konishi Masahiro, Kobayashi Akiko, Kano Masumi

    Proc Annu Meet PSJ   2006 ( 0 ) 102 - 102  2006

     View Summary

    Body temperature (T&lt;sub&gt;b&lt;/sub&gt;) is different between male and female, e.g. daily change in T&lt;sub&gt;b&lt;/sub&gt; is fluctuated with menstruation cycle in female rats. We hypothesized that estrogen plays a crucial role in the sex difference in T&lt;sub&gt;b&lt;/sub&gt;. &lt;B&gt;Methods&lt;/B&gt; (1) Daily change of T&lt;sub&gt;b&lt;/sub&gt; was measured after gonadectomy in male and female rats. After the measurement, silicon tubes containing 17-beta estradiol (E&lt;sub&gt;2&lt;/sub&gt;) crystalline, aimed to maintain blood estrogen constant, were subcutaneously placed in the rats. Then T&lt;sub&gt;b&lt;/sub&gt; measurement was repeated. (2) Thermoregulation during 2-h heat exposure at 34&amp;degC or cold exposure at 5&amp;degC was assessed in gonadectomized female rats, and the same protocol was conducted in those with E&lt;sub&gt;2&lt;/sub&gt; tubes. &lt;B&gt;Results&lt;/B&gt; (1) Compared with male rats, T&lt;sub&gt;b&lt;/sub&gt; rhythm in female gonadectomized rats became unstable, showing 2-4 h irregular oscillations. T&lt;sub&gt;b&lt;/sub&gt; rhythm remained unchanged in male gonadectomized rats. In female gonadectomized rats with E&lt;sub&gt;2&lt;/sub&gt; tubes, T&lt;sub&gt;b&lt;/sub&gt; rhythm returned to the normal level. However, there was no influence of E&lt;sub&gt;2&lt;/sub&gt; on T&lt;sub&gt;b&lt;/sub&gt; rhythm in the male rats. (2) Both in the heat and cold, gonadectomized female rats could not maintain their T&lt;sub&gt;b&lt;/sub&gt; as those with E&lt;sub&gt;2&lt;/sub&gt; tubes. Histological analysis for the rat brain showed that Fos-immunoreactive cells in the hypothalamus were smaller in the rats without E&lt;sub&gt;2&lt;/sub&gt; tubes. &lt;B&gt;Conclusion&lt;/B&gt; These results show that estrogen is involved in the thermoregulation in female rats. Estrogen may modulate thermal sensitivity to the environment at the level of the hypothalamus &lt;b&gt;[J Physiol Sci. 2006;56 Suppl:S102]&lt;/b&gt;

    DOI CiNii

  • Effect of rectal temperature after bath on oxidative DNA damage in human

    SAITO Yasuyo, SAITO Takehito, NAGASHIMA Kei, SUDA Kazuhiro, KANOSUE Kazuyuki, TANAKA Hideto

    人間-生活環境系シンポジウム報告集   30   233 - 236  2006

     View Summary

    Oxidative DNA damage produced by reactive oxygen species (ROS) has been considered as a cause of degenerative processes associated with aging. Generation and reactivity of ROS, which involve chain chemical reactions, depends on body temperature. Thus, ROS would be produced more with increase in body temperature. However, it has yet to be clarified how a change in body temperature modifies the oxidative DNA damage in human. To investigate the effects of rectal temperature after bath on oxidative DNA damage in human, by measuring its biomarker, urinary 8-hydroxydeoxyguanosine (8-OHdG). We determined the urinary 8-OHdG levels of two healthy male volunteers and immersed men at two different water temperatures for 10min. They immersed both in a water bath of 39℃and 42℃ for 10 min, after which exposes 25℃ in the environment for 120 min. The urine 8-OHdG was used at selected time points: pre-, just post-experiment, 2 hr post-experiment, 6 hr post-experiment, 12 hr post-experiment and 24 hr post-experiment. The level of urinary 8-OHdG excretion change significantly compared to each control, at 39℃ did it increase from 2 hr post-experiment, at 42℃ did it increase from just after the bath. The daily average of urinary 8-OHdG excretion, there was a difference in urinary 8-OHdG excretion between 39℃ and the control 42℃ (263±55. 7pmol/kg/day 39℃ vs 454±99. 8pmol/kg/day 42C) . These results suggest that different water temperature produces oxidative DNA damage that might promote body tissue injury.

    CiNii

  • 高張食塩水投与時のラットにおける熱放散反応抑制とそのメカニズムの解析

    小林章子, 永島計

    日本生気象学会雑誌   42 ( 3 ) S50 - S50  2005.10

    CiNii J-GLOBAL

  • 異なる環境温度での運動による深部体温の変化がDNA酸化的損傷(尿中8‐OHdG排泄量)に及ぼす影響

    齋藤恭世, 田中英登, 原川早織, 彼末一之, 永島計, 須田和裕

    日本運動生理学会大会プログラム・抄録集   13th   63  2005

    J-GLOBAL

  • The role of estrogen in the circadian change of body temperature in female rats

    Nagashima Kei, Kobayashi Akiko, Yamanouchi Korehito

    Proc Annu Meet PSJ   2005 ( 0 ) S220 - S220  2005

     View Summary

    It is well known that thermoregulation is different between male and female, although the mechanism remains unclear yet. The purpose of this study is to investigate the role of estrogen on thermoregulatory mechanisms for daily change of body temperature (T&lt;SUB&gt;b&lt;/SUB&gt;). Fist, we measured daily changes in Tb, metabolism, and body surface temperature in gonadectomized male and female rats (n=6 each) under 12:12h light-dark condition. Compared with normal rats, T&lt;SUB&gt;b&lt;/SUB&gt; in the female gonadectomized rats became unstable, showing 2-4 h irregular small oscillation, although chi-square analysis indicated that the period of the rhythm remained 24 h. However, there was no change in the T&lt;SUB&gt;b&lt;/SUB&gt; rhythm in the male gonadectomized rats. Second, silicon tubes containing E2 crystalline were subcutaneously implanted in the gonadectomized rats. In the female gonadectomized rats with the tubes, the T&lt;SUB&gt;b&lt;/SUB&gt; rhythm was restored to the normal pattern. However, there was no influence of E2 on the T&lt;SUB&gt;b&lt;/SUB&gt; rhythm in the male rats. The T&lt;SUB&gt;b&lt;/SUB&gt; change due to the gonadectomy is closely linked with both metabolism and body surface temperature. These results indicate that E2 is involved in the thermoregulation for daily change of T&lt;SUB&gt;b&lt;/SUB&gt; in female rats, but not in male rats. We also hypothesized that central neurons reacting both thermal and E2 stimuli would be closely associated with the mechanism. Therefore, histological analysis was conducted to test the hypothesis. &lt;b&gt;[Jpn J Physiol 55 Suppl:S220 (2005)]&lt;/b&gt;

    DOI CiNii

  • New system for the analysis of thermal comfort; multi-point mesurement of skin temperatures and sensations,and their visualization.

    Nakamura Mayumi, Esaki Hidenori, Osawa Naoki, Kobayashi Akiko, Konishi Aki, Yasuhara Saki, Nagashima Kei, Kanosue Kazuyuki

    Proc Annu Meet PSJ   2005 ( 0 ) S221 - S221  2005

     View Summary

    The sensation related to temperature is complex in that there are two different kinds of sensation, subjective &amp;ldquo;thermal comfort&amp;rdquo; and objective &amp;ldquo;temperature sensation&amp;rdquo;, and in that we feel local as well as whole body sensations. And it is not clear how these different sensations are related with each other. To investigate this question we developed a new system to measure the distribution of whole body skin temperature and the two sensations in human. Skin temperatures (Tsk) of in total 50 points on both sides of the body were measured by thermocouples, and local heat flux was also measured at 25 points by heat flux sensors. Core temperature (Tcore) was measured by telemetric system (CoreTemp), the transmitter of which was swallowed by a subject. Temperature sensation (cold-neutral-hot) and thermal comfort (uncomfortable-neutral-comfortable) of 25 points of body surface, and those of the whole body were reported by the subject using a console with 52 dials for each sensation. Color-coded Tsk and sensations were displayed on the computer display. This system enables to see the distribution and change of Tsk and sensations over the body surface even with clothes on, and should be useful for the analysis of temperature-related sensations as well as the design of comfortable environment or clothes. &lt;b&gt;[Jpn J Physiol 55 Suppl:S221 (2005)]&lt;/b&gt;

    DOI CiNii

  • P-715 皮膚温、局所的温冷感・快適感の多点計測とデータ可視化システムの開発(Session7 (Poster Session2))

    中村 真由美, 江崎 秀範, 大澤 直樹, 小林 章子, 小西 あき, 安原 祥, 永島 計, 彼末 一之

    人間-生活環境系シンポジウム報告集   5   A60  2005

    CiNii

  • 運動と体温調節 体温調節のメカニズム

    彼末一之, 永島計, 小西あき

    体育の科学   54 ( 10 ) 764 - 768  2004.10

    J-GLOBAL

  • 体温調節のメカニズム (特集 運動と体温調節)

    彼末 一之, 永島 計, 小西 あき

    体育の科学   54 ( 10 ) 764 - 768  2004.10

    CiNii

  • Coordination of homeostasis by the circadian clock

    Kanosue Kazuyuki, Nagashima Kei, Ishida Norio

    Proc Annu Meet PSJ   2004 ( 0 ) S7 - S7  2004

     View Summary

    Various homeostatic systems are under the control of the circadian clock. Recently we found that fasted rats kept under a light-dark cycle showed a decrease in body temperature (Tcore) only during the light phase. In the fasting condition, in the dark phase Tcore was maintained normal by a suppression of heat loss mechanism despite the reduction of metabolic heat production. In contrast, the response was weakened in the light phase, decreasing Tcore greatly, but in this period cold escape behavior is facilitated, probably for the compensation of Tcore decrease. These modulations of thermoregulatory effectors, depending on the time of the day, are considered as adaptive responses to the lack of food. Interestingly, in the rats with the lesion of the hypothalamic suprachiasmatic nucleus in which daily rhythms of Tcore and activity were abolished, body temperature was unchanged by food deprivation. Very similar loss of Tcore modulation was observed in those mice lacking the criptochrome genes (Cry1&lt;sup&gt;&amp;minus;/-&lt;/SUP&gt;/Cry2&lt;sup&gt;&amp;minus;/-&lt;/SUP&gt;) during food restriction. These results suggest that the circadian clock is a key structure for coordinating the controls of body temperature, energy metabolism, and behavior. &lt;b&gt;[Jpn J Physiol 54 Suppl:S7 (2004)]&lt;/b&gt;

    DOI CiNii

  • Reduction in body temperature during fasting in rats

    Nagashima Kei

    Proc Annu Meet PSJ   2004 ( 0 ) S56 - S56  2004

     View Summary

    Kei Nagashima, Dept Physiol, Waseda Univ Sch Human Sci&lt;Br&gt;&lt;B&gt;A reduction in body temperature during fasting in rats&lt;/B&gt;&lt;Br&gt;Body temperature (T&lt;sub&gt;b&lt;/sub&gt;) is determined by the balance between heat production and heat loss. In rats, T&lt;sub&gt;b&lt;/sub&gt; is linked with heat production during free-feeding. Fasting reduces the production of heat throughout a day; however, T&lt;sub&gt;b&lt;/sub&gt; is maintained during the dark phase and decreases markedly during the early light phase. The reduction in T&lt;sub&gt;b&lt;/sub&gt; would protect animals from excessive heat loss, minimizing the temperature difference between the body and environment. Temperature of the tail, reflecting active heat loss, also decreases during the fasting. These results indicate that rats suppress heat loss to maintain T&lt;sub&gt;b&lt;/sub&gt;. However, the suppression is weaker in the light phase than dark phase, which would be a mechanism involved in the reduction in T&lt;sub&gt;b&lt;/sub&gt;. Although it still remains unclear if rats control the phase-specific change in thermoregulation, a couple of evidences proving the possibility are presented in this symposium. When the suprachiasmatic nucleus (SCN, the master clock for circadian rhythm) are lesioned, the reduction in T&lt;sub&gt;b&lt;/sub&gt; does not occur, although metabolic heat production decreases in the same manner as the sham-operated rats. Moreover, when rats are exposed to constant darkness, the reduction is attenuated and the phase of the reduction is delayed. These results suggest that the reduction of T&lt;sub&gt;b&lt;/sub&gt; occurs in response to the stimulus originating the SCN. Moreover, the light is an important factor for this response. In summary, the reduction of T&lt;sub&gt;b&lt;/sub&gt; during low energy storage would be a regulated phenomenon linked with biological rhythm. &lt;b&gt;[Jpn J Physiol 54 Suppl:S56 (2004)]&lt;/b&gt;

    DOI CiNii

  • Lesions of the median preoptic nucleus abolish the increase in operant heat-escape/warm-seeking behavior after hypertonic-saline injection

    Konishi Masahiro, Asano Kento, Nagashima Kei, Kanosue Kazuyuki

    Proc Annu Meet PSJ   2004 ( 0 ) S230 - S230  2004

     View Summary

    We previously reported that s.c. hypertonic-saline increased operant heat-escape/warm-seeking behavior in rats. In this study, to assess the mechanism involved, the behavior was measured in rats of which the median preoptic nucleus (MnPO) was chemically ablated (ibotenic acid, 5 &amp;mu;g). The lesion was verified success by an attenuation of the drink after i.c.v. injection of angiotensin II. Thirty minutes after s.c. injection of either 2500- or 154-mM saline (1.0 ml/100 g), rats were successively placed at 26, 35 and 40&amp;deg;C for 1 h each. They could trigger 0&amp;deg;C-air for 45s when entered a specific area (i.e. operant behavior). In the sham-operated rats, counts of the operant behavior was greater (&lt;I&gt;p&lt;/I&gt;&lt;0.05) in 2500-mM than 154-mM saline during 35 and 40&amp;deg;C-heat. However, in the lesioned-rats, the counts did not differ between the two trials, and were at the same level as those in the sham-operated rats in 154-mM saline trial. These results suggest that the MnPO is involved in the mechanism which activates heat-escape/warm-seeking behavior after hypertonic-saline injection. Howeover, the MnPO is not associated with the behavior in euhydrated condition. &lt;b&gt;[Jpn J Physiol 54 Suppl:S230 (2004)]&lt;/b&gt;

    DOI CiNii

  • 行動性体温調節(高齢者の発達を支援する環境づくり(5))

    永島 計

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  • チーム医療・看護とコメディカルの教育 64. 臨床検査技師教育における臨床生理検査‐3役ローテーション教育‐

    依藤史郎, 東照正, 川野淳, 別府慎太郎, 今岡弘之, 永島計, 佐川節子, 石蔵文信

    医学教育   34 ( Suppl. ) 41  2003.07

    J-GLOBAL

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    依藤 史郎, 東 照正, 川野 淳, 別府 慎太郎, 今岡 弘之, 永島 計, 佐川 節子, 石蔵 文信

    医学教育   34 ( Suppl. ) 41 - 41  2003.07

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    K Kanosue, A Taniguchi, K Yoshida, K Nagashima

    FASEB JOURNAL   17 ( 4 ) A26 - A26  2003.03

    Research paper, summary (international conference)  

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    小西 正博, 浅野 健人, 永島 計, 彼末 一之

    日本生気象学会雑誌   39 ( 3 ) S35 - S35  2002.10

    CiNii

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    J-GLOBAL

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    日本神経科学大会プログラム・抄録集   25th   216  2002.07

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    日本神経科学大会プログラム・抄録集   25th   217  2002.07

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    KANOSUE Kazuyuki, NAGASHIMA Kei, TANIGUCHI Aki, KONISHI Masahiro

    J. Jpn. Res. Assoc. Text. End-Uses   43 ( 3 ) 17 - 22  2002.03

    DOI CiNii

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    T Yagishita, K Nagashima, T Okada, N Sadato, Y Yonekura, K Kanosue

    FASEB JOURNAL   16 ( 4 ) A40 - A40  2002.03

    Research paper, summary (international conference)  

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    S Liu, M Maruyama, K Nagashima, K Kanosue

    FASEB JOURNAL   16 ( 4 ) A40 - A40  2002.03

    Research paper, summary (international conference)  

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    Biomed Thermology   21 ( 2 ) 38 - 44  2001.10

    CiNii J-GLOBAL

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    快適性を考えるシンポジウム   22nd   1 - 6  2001.10

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    Clin Neurosci   19 ( 10 ) 1129 - 1131  2001.10

    J-GLOBAL

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    日本神経科学大会プログラム・抄録集   24th ( 2-3 ) 332 - 388  2001.09

    J-GLOBAL

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    八木下知子, 岡田智久, 定藤規弘, 永島計, 細野剛良, 米倉義晴, 彼末一之

    日本神経科学大会プログラム・抄録集   24th ( 2-3 ) 345 - 401  2001.09

    J-GLOBAL

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    永島計, 松江健太, 彼末一之

    日本神経科学大会プログラム・抄録集   24th ( 2-3 ) 234 - 290  2001.09

    J-GLOBAL

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    谷口あき, 陳小明, 永島計, 彼末一之

    神経化学   40 ( 2/3 ) 388  2001.09

    J-GLOBAL

  • 機能的MRIを用いた局所温・冷刺激による脳内賦活領域の検討

    八木下知子, 岡田智久, 定藤規弘, 永島計, 細野剛良, 米倉義晴, 彼末一之

    神経化学   40 ( 2/3 ) 401  2001.09

    J-GLOBAL

  • 絶食による体温の慨日リズム変動のメカニズム

    永島計, 松江健太, 彼末一之

    神経化学   40 ( 2/3 ) 290  2001.09

    J-GLOBAL

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    彼末一之, 永島計, 細野剛良, 丸山めぐみ, 谷口あき, 定藤規弘, 岡田知久, 米倉義晴

    日産科学振興財団研究報告書   24   29 - 32  2001.08

    J-GLOBAL

  • 温熱的快・不快感(暑さ・寒さ)の脳内機序

    彼末一之, 永島計, 八木下知子, 谷口あき, 細野剛良

    Biomed Thermology   21 ( 1 ) 19 - 19  2001.06

    CiNii J-GLOBAL

  • Increased heat-escape/cold-seeking behavior following hypertonic saline injection in rats

    K Nagashima, M Konishi, S Nakai, K Kanosue

    FASEB JOURNAL   15 ( 5 ) A1107 - A1107  2001.03

    Research paper, summary (international conference)  

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    西麻衣子, 陳小明, 永島計, 柴田政章, 彼末一之

    日本生理学雑誌   63 ( 1 ) 34 - 34  2001.01

    J-GLOBAL

  • 体温概日リズムにおける迷走神経の役割

    松江健太, 永島計, 中井定, 彼末一之

    日本生理学雑誌   63 ( 1 ) 34 - 34  2001.01

    J-GLOBAL

  • 温熱的不快感(暑さ・寒さ)の脳機構の解析

    彼末 一之, 永島 計, 細野 剛良

    研究報告書   24   29 - 32  2001

    CiNii

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    中井定, 永島計, 彼末一之

    日本神経科学大会プログラム・抄録集   23rd   78  2000.09

    J-GLOBAL

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    日本神経科学大会プログラム・抄録集   23rd   77  2000.09

    J-GLOBAL

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    田中睦美, 永島計, 彼末一之, ロビン マッカーレン

    日本神経科学大会プログラム・抄録集   23rd   78  2000.09

    J-GLOBAL

  • 温熱的快・不快感(寒さ)に関わる脳部位のfMRIによる検索

    彼末一之, 定藤規弘, 岡田知久, 細野剛良, 永島計, 依田珠江, 吉田恭子, 中井定, 米倉義晴

    日本神経科学大会プログラム・抄録集   23rd   304  2000.09

    J-GLOBAL

  • 体温調節性行動に対する絶食の影響

    依田珠江, 西麻衣子, 丸山めぐみ, 劉そ, 永島計, 彼末一之

    日本神経科学大会プログラム・抄録集   22nd   237  1999.07

    J-GLOBAL

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    吉田康子, 丸山めぐみ, 細野剛良, 永島計, 彼末一之

    日本神経科学大会プログラム・抄録集   22nd   324  1999.07

    J-GLOBAL

  • Affects of age and body weight on hemopoiesis by administration of rHuEPO.

    神吉豊, 伊東正文, 村山祐一郎, 永島計, 和田行雄, 岡隆宏, 藤田直久, 横山繁樹, 渡部高久

    京都府立医科大学雑誌   108 ( 5 ) 555 - 564  1999.05

    J-GLOBAL

  • 年齢,体重がエリスロポイエチン(rHuEPO)投与下の造血能に及ぼす影響

    神吉 豊, 伊東 正文, 村山 祐一郎, 永島 計, 和田 行雄, 岡 隆宏, 藤田 直久, 横山 繁樹, 渡部 高久

    京都府立医科大学雑誌   108 ( 5 ) 555 - 564  1999.05

     View Summary

    1)造血能亢進程度に年齢ならびに体重による有意差は認めなかった. 2)rHuEPO投与及び貯血による重篤な合併症は認めなかった

  • Hemopoiesis with the administration of rHuEPO.

    神吉豊, 伊東正文, 村山祐一郎, 永島計, 和田行雄, 岡隆宏, 藤田直久, 横山繁樹, 渡部高久

    京都府立医科大学雑誌   108 ( 4 ) 439 - 445  1999.04

     View Summary

    予定開心術症例12例に対し,rHuEPO;200U/kgを術前14日目鉄剤と共に静脈内に連日投与した結果,全例でHb濃度が増加したが,症例によりその増加の程度に差を認めた.この原因を探るべく,術前状態及び術前諸検査値とHb増加濃度との相関関係を検討したが,決定的な関係を見いだすことはできずこれらからrHuEPO投与によるHbの増加の程度を投与前に推定することは困難と思われた.また,網赤血球産生指数及び骨髄像からみて明らかに造血能は亢進しているにも関わらずHb濃度の増加にかならずしも反映されず,以前から言われているもの以外にも造血能亢進を妨げる何らかの因子が存在している可能性が示唆された

    J-GLOBAL

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    K Nagashima, ER Nadel, J Wu, RD Wemple, SA Kavouras, GW Mack

    FASEB JOURNAL   12 ( 4 ) A125 - A125  1998.03

    Research paper, summary (international conference)  

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    AM Stahl, GW Mack, K Nagashima, T Morocco, ER Nadel

    FASEB JOURNAL   12 ( 4 ) A390 - A390  1998.03

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    宇宙利用シンポジウム   13th   55 - 58  1996.09

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    長崎大学熱帯医学研究所共同研究報告集   1995   136  1996.06

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    小野スポーツ科学   4   109 - 122  1996

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    相馬彰, 永島計, 大川原潤, 戸田省吾, 北浦一弘, 佐藤伸一, 和田行雄, 岡隆宏

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    永島 計, 和田 行雄, 河合 隆寛

    胸部外科   47 ( 12 ) 1026 - 1028  1994.11

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    永島計, 和田行雄, 河合隆寛, 足立省三, 春藤啓介, 岡隆宏

    胸部外科   47 ( 12 ) 1026 - 1028  1994.11

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    永島計, 田中承男, 中江あきら, 松井英, 岡隆宏

    日本臨床外科医学会雑誌   55 ( 10 ) 2547 - 2550  1994.10

     View Summary

    38歳女,胸部X線異常陰影と同時期より続く乾性咳を主訴とした。上前縦隔良性腫瘍の診断の下に胸骨縦切開にて摘出手術を行った。腫瘍は反回神経分岐より中枢の左迷走神経本幹より発生していた。腫瘍に接した神経束の腹側を長軸方向に部分切除し,迷走神経を切離せずに被膜を含め腫瘍を摘出した。術後嗄声等の合併症は認めず,組織診断で神経鞘腫であった

    DOI CiNii J-GLOBAL

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    芳田哲也, 中井誠一, 寄本明, 永島計, 河端隆志, 森本武利

    日本生気象学会雑誌   31 ( 3 ) 113  1994.10

    DOI J-GLOBAL

  • 101 寒冷暴露時の尿中Na排泄に与える体力水準の影響

    芳田 哲也, 中井 誠一, 寄本 明, 永島 計, 河端 隆志, 森本 武利

    日生気誌   31 ( 3 ) 113 - 113  1994

    DOI CiNii

  • Use of erythropoietin for predonated autologous transfusion. Further study on administrating doses of erythropoietin specially referred to side effects, age and body weight.

    神吉豊, 村山祐一郎, 伊東正文, 永島計, 北浦一弘, 西山勝彦, 和田行雄, 横山繁樹, 渡部高久

    自己血輸血   6 ( 2 ) 152 - 157  1993.12

    J-GLOBAL

  • Evaluation of bone marrows of the patients treated with erythropoietin (KRN 5702).

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    自己血輸血   5 ( 1 ) 78 - 82  1992.10

    J-GLOBAL

  • Study on iron administration method under using recombinant human erythropoietin(rHuEPO).

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  • 人工心肺中の抗凝固法の検討

    永島 計

    日本胸部外科学会雑誌   40 ( 臨増 ) 1600 - 1600  1992.09

  • A Case of intractable massive ascites (hepatic lymphorrhea) following radical gastrectomy for early gastric cancer.

    清水義博, 田中承男, 中江あきら, 糸井啓純, 松井英, 永島計

    京都府立医科大学雑誌   101 ( 2 ) 175 - 180  1992.02

    J-GLOBAL

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    永島 計

    日本胸部外科学会雑誌   39 ( 臨増 ) 1595 - 1595  1991.09

  • Modified : significance of SAGM introduction.6.Modified SAGM used in preoperative blood collection for non-blood transfusion open heart surgery.

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    日本輸血学会雑誌   37 ( 4 ) 555 - 558  1991.09

    J-GLOBAL

  • 第54回日本肺癌学会関西支部会 : 70.急速な増大を示した胸腔内腫瘍の1例

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    肺癌   31 ( 3 ) 452 - 452  1991.06

    CiNii

  • Therapeutic effect of intraperitoneal administration of CDDP and OK-432 in peritonitis carcinomatosa cases of gastric carcinoma.

    田中承男, 糸井啓純, 松井英, 中江あきら, 永島計

    癌と化学療法   18 ( 4 ) 661 - 663  1991.04

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  • 体外循環後の自己新鮮凍結血漿投与についての検討 凝固線溶系の変化を中心に

    永島 計

    日本外科学会雑誌   92 ( 臨増 ) 388 - 388  1991.02

  • A case of acinar cell carcinoma of the pancreas in association with a liver metastasis and multiple early gastric cancers.

    大森浩二, 田中承男, 河村允, 中江あきら, 永島計, 木本邦彦, 関川進

    日本消化器外科学会雑誌   22 ( 11 ) 2701 - 2704  1989.11

    DOI J-GLOBAL

  • A CASE OF ACINAR CELL CARCINOMA OF THE PANCREAS IN ASSOCIATION WITH A LIVER METASTASIS AND MULTIPLE EARLY GASTRIC CANCERS

    OHMORI Kohji, TANAKA Tsuguo, KAWAMURA Makoto, NAKAE Akira, NAGASHIMA Kei, KIMOTO Kunihiko, SEKIKAWA Susumu

    The Japanese journal of gastroenterological surgery   22 ( 11 ) 2701 - 2704  1989.11

     View Summary

    膵腺房細胞癌はまれな腫瘍であるが,機能性腫瘍として外分泌作用を生じることもあるといわれている.予後に関しては悲観的な報告が多い.当院で,多発早期胃癌に併存し肝転移をきたしていた微小膵腺房細胞癌に対し胃全摘,膵尾部切除,肝切除を行い術後1年再発なく経過した症例を経験したので報告する.

    DOI CiNii

  • 総動脈幹症Collett-Edwards 2型の1治験例

    永島 計

    日本胸部外科学会雑誌   37 ( 5 ) 1070 - 1070  1989.05

  • Clinical studies on surgical treatment for hepatic metastasis.

    大森浩二, 永島計, 中江あきら, 河村允, 田中承男, 金田悌二郎

    交通医学   43 ( 3 ) 165 - 171  1989.05

    J-GLOBAL

  • Pre- and post-operative complications in aged patients with gastric cancer.

    田中承男, 永島計, 大森浩二, 中江あきら, 河村允, 金田悌二郎

    交通医学   43 ( 3 ) 160 - 164  1989.05

    J-GLOBAL

  • 当院における経皮血管形成術症例の検討

    永島 計

    交通医学   43 ( 2 ) 80 - 80  1989.03

  • A case of acinar cell carcinoma of the pancreas in association with a liver metastasis and multiple early gastric cancers.

    OHMORI Kohji, TANAKA Tsuguo, KAWAMURA Makoto, NAKAE Akira, NAGASHIMA Kei, KIMOTO Kunihiko, SEKIKAWA Susumu

    Jpn J Gastroenterol Surg, Nihon Shokaki Geka Gakkai zasshi   22 ( 11 ) 2701 - 2704  1989

    DOI CiNii

  • Therapeutic effect of intraperitoneal administration of CDDP and OK-432 in peritonitis carcinomatosa cases.

    田中承男, 大森浩二, 永島計, 中江あきら, 河村允, 金田悌二郎

    交通医学   42 ( 6 ) 297 - 301  1988.11

    J-GLOBAL

  • 当院外科における高齢者手術症例について

    永島 計

    交通医学   42 ( 2 ) 106 - 106  1988.03

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Industrial Property Rights

  • 熱障害危険度判定方法およびウエアラブルセンサシステム

    特許6520846

    永島 計

    Patent

  • 熱障害危険度判定方法およびウェアラブルセンサシステム

    永島 計

    Patent

  • 熱障害危険度判定方法およびウエアラブルセンサーシステム

    永島 計

    Patent

Awards

  • 日本生理学会久野賞

    1999  

Research Projects

  • Physiology and Psychology of Humidity and Wetness

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Project Year :

    2019.04
    -
    2023.03
     

  • Mechanisms involved in heat tolerance due to depletion of estradiol secretion

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Project Year :

    2016.04
    -
    2018.03
     

    Nagashima Kei, Marui Shuri

     View Summary

    The present research aimed, (1) to develop a mouse model for evaluating the thermal tolerance of postmenopausal women, ② to test if decrease of estradiol secretion augments a risk of heat stroke (autonomic reaction, behavioral reaction, temperature sensation, cognitive function and behavior related to the heat), and it affects time dependence or not. We completed a system to evaluate the heat escape behaviors of mice and at the same time evaluated metabolic rate under heat. Estradiol contributed to the decrease in metabolic rate at the time of heat loading, and it seemed that this decrease is related to the decrease of the heat resistance. Also, its effect was noticeable in the light period. It was thought that the heat escape behavior was not affected.

  • Clarifying mechanisms involved in thermal pleasantness/unpleasantness and the application for evaluation of the inside environment of clothes

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    2013.04
    -
    2018.03
     

    Nagashima Kei, Marui Shuri, Aizawa Yuka

     View Summary

    ① Development of a method to experimentally reproduce thermal discomfort, ② Development of a method to reproduce ① in the brain function imaging device (fMRI) and obtain brain function image. Hypothesis verification that temperature sensation sensation and thermal discomfort feeling are processed in different brain regions. ③ Eluciation of the mechanism of transformation of thermal discomfort seen during exercise and dehydration from the changes in deep body temperature and skin temperature. For ①, regional temperature difference of the body surface can be reproduced by constructing it by a combination of rise and fall of body surface skin temperature with water perfusion suit and local skin temperature stimulation. ② Different brain regions were involved in temperature perception (discriminative and hedonic components). ③ Possible contribution to the thermal discomfort feeling other than the temperature parameter per se was clarified.

  • Development of a method of reducing heat strain at recovery work for destroyed Nuclear Power Plant and construction work in Summer

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    2013.04
    -
    2018.03
     

    Sawada Shin-ichi, OKA Tatsuo, YASUDA Akinori, TAI Tetsuo, SON Su-young, IDA Hirobumi, NAKAYAMA Kazumi, WADA Jun

     View Summary

    To reduce heat stress and strain at recovery work for the destroyed Nuclear Power Plant and construction work in summer, we developed a cooling method using a blower fan and a water spray (plus additional water immersion of extremities) and demonstrated its certain effectiveness in the laboratory experiment. Therefore, to examine the effectiveness at real outdoor work sites next, a commercially available mist fan having a cooling mechanism similar to our method was substituted for our experimental model to investigate how much effective the cooling method is for reducing the environmental heat stress at a construction work site. The study showed that the combined use of fan and water spray drastically decreased the WBGT value which is an indicator of heat stress suggesting that this method has remarkable cooling effect for reducing the environmental heat stress at outdoor work. Furthermore, the optimum distance between the object to be cooled and the mist fan could also be identified.

  • Comprehensive Analysis of the mechanism involved in changes of thermoregulatory responses during dehydration

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    2008
    -
    2011
     

    NAGASHIMA Kei, KANOSUE Kazuyuki, TOKIZAWA Ken, UCHIDA Yuki, LIN Chen_hsien, MATSUDA Mayumi

     View Summary

    Dehydration is a factor deteriorating autonomic thermoregulatory responses, which could induce heat stroke. However, the central mechanism remains unclear yet. Moreover, we do not know about the influence of dehydration on behavioral thermoregulation. In the present study, we clarified that the median preoptic nucleus in the hypothalamus is important as the receptor site of plasma osmolality, and it suppresses thermal reception of the median preoptic nucleus(thermal reception site in the central). The heat escape/cold seeking behavior in mice is suppressed in the condition of dehydration, and thermal sensation to heat was also decreased in dehydrated humans. These results indicate a possible mechanism involved in heat stroke.

  • Clarification of the mechanisms involved in circadian body temperature rhythm and its physiological significance

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    2005
    -
    2007
     

    NAGASHIMA Kei, KABE Akiyoshi, TOKIZAWA Ken

     View Summary

    The goal of present study was to clarify the mechanism involved in generation of circadian body temperature rhythm. In the research, we hypothesized that 1) the suprachiasmatic nucleus (SCN), i. e. the central for circadian rhythms modulates thermosensitiveity at the levels of the central and periphery, and 2) the body temperature rhythm is important in synchronizing the rhythms of the central and periphery. In experiments, mice were exposed to the cold at 20℃ after ad-lib feeding or 48-h fasting, then we estimated metabolic heat production and UCP1 mRNA in the brown adipose tissue. In addition, cFos expression reflected by neural activity in the hypothalamus was also estimated. The same experimental protocol was repeated on Clock mutant mice, which lacks normal circadian rhythmicity. During ad-lib feeding, there were no differences in thermoregulatory responses and cFos expression in the cold. However, during fasting, heat production response was apparent only in the dark phase, and cFos expression in the SCN was augmented in the light phase. There were no phase-differences in the responses in Clock mutant mice. We also found an inhibitory network from the SCN and paraventricular nucleus associated with the sympathetic nerve activity. The SCN was influenced by environmental temperature and feeding condition, which is involved in time-dependency of body temperature regulation.

  • 体温調節機構の性分化メカニズムの解析

    日本学術振興会  科学研究費助成事業 特定領域研究

    Project Year :

    2005
    -
    2006
     

    永島 計

     View Summary

    目的 女性ホルモンはホットフラッシュ、冷えなどの女性特有な体温調節、感覚異常に関係していると考えられている。しかしながら、その明確なメカニズムは明らかではない。本年度は、女性ホルモンがi)体温概日リズムにいかに影響を与えるか,ii)暑熱、寒冷暴露時の体温調節反応にいかに影響を与えるか iii)性周期に応じて体温調節反応、温度感覚はいかに変化するかを調べるとともに、そのメカニズムを検討した。i,iiについては動物実験にて検証し、血については人での実験を行った。方法と結果 i)卵巣摘出ラットを用いて1週間の体温の変更を無拘束に記録した。約24時間の周期性は認めたが、3-4時間周期の大きな体温変動成分が現れるリズム変調が生じた。この変調は皮下に17beta-estradiol含有シリコンチューブを埋め込むことにより改善した。ii)卵巣摘出ラット、および17イ-estradiol含有シリコンチューブ皮下埋め込みによるエストロゲン補充を行ったラットの33℃,5℃の環境温での耐暑、耐寒反応を比較した。耐暑、耐寒反応とも卵巣摘出ラットでは低下しており体温が各々エストロゲン補充ラットに比べて、上昇もしくは低下した。このメカニズムを明らかにするために、視床下部でのcFOS発現を調べた。暑熱下では内側視索前野、寒冷下では背内側核に多くの発現画あったが、体温の変動は少ないのにも関わらず、エストロゲン補充ラットで多くのcFOS発現が認められた。iii)28℃の温度中性域から、23℃の中度寒冷環境下に若年女性を、黄体期、卵胞期に各々暴露した。深部温は、28℃,23℃とも黄体期に高かったが、温度感覚は同一であった。結語 女性ホルモンは,体温調節に大きな影響を与える。また、このメカニズムとして、中心温に対する脳での温度感受性のエストロゲンの変化であると予想した。

  • Analysis of functional connection of the dorsomedial hypothalamic nucleus for themoregulation

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    2003
    -
    2004
     

    KANOSUE Kazuyuki, NAGASHIMA Kei

     View Summary

    The preoptic area(POA) in the hypothalamus occupies a crucial position in the neuronal circuit for thermoregulation. Recently, the dorsomedial hypothalamus(DMH) was reported to be involved in the control of heat production. Therefore, in the present study, we investigated the brain areas that had functional afferent connections with the dorsomedial hypothalamus(DMH) for thermoregulation in rats, using immuno-histochemical analyses of a retrograde tracer, cholera toxin-b(CTb), injected into the DMH and Fos expression in response to warm (33℃) or cold (10℃) exposure. A double-labeled cell was defined as the neuron had functional connection with the DMH in relation to thermoregulation. In the warm-exposed rats, double-labeled cells were obtained only in the median preoptic area(MnPO). In the cold exposed rats, double-labeled cells were obtained in the anterior hypothalamus(AH), especially in its medial and ventral parts. The results in the Fos and CTb studies raised a possibility that the DMH neurons would be inhibited by the MnPO neurons, and CTb-IR neurons in the MnPO would be GABAergic and directly regulate activity of the DMH neurons. To examine this possibility, we further investigated an expression of mRNA for GAD67,a marker for GABAergic neuron, in CTB-IR MnPO neuron. We found that 79.6% of CTb-IR cells in the MnPO exhibited signals for GAD67 mRNA. These results suggest that the neurons in the POA, especially in the MnPO, receive warm inputs from the skin and send inhibitory signals, via GABAergic synapses, to the DMH to suppress heat production, and the neurons in the AH receive cold signals from the skin and send excitatory signals to the DMH, to activate heat production.

  • Analysis of changes in body temperature rhythm during fasting

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Project Year :

    2002
    -
    2003
     

    NAGASHIMA Kei

     View Summary

    It is well known that body temperature (T_b) follows a circadian rhythm. Despite recent advance in knowledge of thermoregulation, we know only a little about the mechanism of the T_b rhythm. In this symposium, we show several evidences proving that i) the prime mechanism for the circadian T_b rhythm is a change in characteristics of thermoregulatory processes according to circadian phases, ii) clock genes and the suprachiasmatic nucleus are involved in the mechanism.
    T_b rhythm during fasting. In ad-lib feeding rats, T_b rhythm was linked with heat production rhythm. Moreover, tail surface temperature reflecting active heat loss also changed linearly with the T_b rhythm. Thus it is thought that heat production dominantly determines the T_b rhythm in fed condition. Although heat production decreased and its circadian amplitude was blunted during 3-day fasting, the T_b rhythm was well maintained. Moreover, the tail temperature decreased greatly in the active phase. These results suggest that animals control their circadian T_b rhythm by modulating thermoregulatory responses, which depends on feeding and/or nutritional states.

  • 「冷え症」の生理学的機序の研究

    日本学術振興会  科学研究費助成事業 萌芽研究

    Project Year :

    2002
    -
    2003
     

    彼末 一之, 永島 計, 細野 剛良, 大野 ゆう子

     View Summary

    「冷え性」は一般に良く使われているが、その病態はほとんど研究されていない.これまで行われているのは主として漢方医学の方面からのアプローチで、そこでは「末梢血管運動障害」と捉えられることが多い.本研究では冷え性の病態を生理学的に明らかにすることを目的に実験を行った。あらかじめインタビューから冷え症と考えている女性を中程度の寒冷(23℃)に曝露すると、冷え症ではない女性に比べて同じ深部温、皮膚温の条件でもより強く「寒さ」を申告した.また冷え症の女性は甲状腺ホルモン(T4)が正常範囲ではあるが非冷え症群に比べて低く、代謝量も低い傾向にあった.つまり「冷え症」の女性は正常範囲とはいえ、甲状腺機能が低下している.その結果代謝量(熱産生量)が低くなる.すると体温を維持するために2つの反応が起こる.(1)熱放散を抑えるために皮膚血管が収縮する.これは特に動静脈吻合の発達した四肢末端部(手足)から起こる.皮膚血管の収縮は皮膚温の低下を招き、その結果末梢部で「冷え」を感ずるようになる.(2)一方、自律系による体温の調節不全(代謝量低下)は代償的に体温調節行動(寒冷逃避行動)を促進する.それは寒冷への感覚の感度亢進という形で現れるであろう.以上のように冷え性は代謝の低下に対する適応反応と考えられる。一人で生活する場であれば、温度環境をその人にとって快適に設定することは可能である.しかし多人数が同じ空間を共有する公共の場では温度環境は平均的な人たちにとっての快適なものに設定されがちである.すると少数グループになるであろう「低代謝」の人たちは冷え症を訴える結果になる.公共の場の環境設定にも個人差を考慮することが必要である。

  • Analysis of efferent projection from the preoptic area for the control of thermoregulatory effector responses.

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

    Project Year :

    2000
    -
    2002
     

    KANOSUE Kasuyuki, NAGASHIMA Kei, DOHI Yoshitane, INAGAKI Shinobu

     View Summary

    The preoptic area (PO) plays a key role in body temperature regulation by integrating information about local brain temperature and other body temperatures, and by sending efferent signals to various effector organs. Our previous studies elucidated that thermoregulatory skin vasodilation is elicited by the activation of warm-sensitive neurons in the PO, while shivering and non-shivering thermogenesis are controlled by the inhibitory actions of warm-sensitive neurons. In the midbrain skin vasodilative neurons were located in the the ventrolateral portion of the rostral periaqueductal grey (rPAG), and neurons having excitatory effect on non-shivering thermogenetive are located in the ventrolateral part of the caudal PAG (cPAG). However, the direct connections between the PO or the rest of the hypothalamus and the PAG in terms of thermoregulation has not been fully documented. In the present study we investigated in the rat's brain the distribution of neurons that are activated by exposure to neutral (26 ゜C), warm (33 ゜C) or cold (10 ゜C) ambient temperature, and that also project to the rPAG or cPAG, by the immunohistochemical analysis of Fos and a retrograde tracer, cholera toxin- b (CTb). When the tracer injection was centered in the rPAG, many double-labeled cells were observed in the dorsal part of the median preoptic nucleus (MnPO) in warm-exposed rats but few double-labeled cells were observed in the MnPO in cold-exposed rats. On the other hand, when the tracer injection was centered in the cPAG, many double-labeled cells were seen in the the posterior hypothalamic area (PH) in the cold-exposed rats but few double- labeled cells were seen in the warm-exposed rats. These results suggest that the rPAG receives warm signals from the MnPO and the cPAG receives cold signals from the PH region, probably participating in the efferent pathway for thermoregulatory skin vasomotion and non-shivering thermogenesis, respectively.

  • Relationship between exercise-induced hypervolemia and albumin synthesis and distribution in the body

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

    Project Year :

    2000
    -
    2001
     

    NAGASHIMA Kei

     View Summary

    [Background] It is well known that exercise training is a potent stimulus to increase blood volume (BV), which is determined by many factors, i.e. renal water and Na^+ handling and fluid movement between the cellular and extracellular spaces. However, it remains unknown which factor is involved in the mechanism for the increase in BV due to exercise training.
    [Purpose] 1. Treadmill exercise can increase BV in rats -rats could be model animal to investigate the mechanism for BV increase-?
    2. Albumin, major substance maintaining plasma oncotic pressure, could be a factor increasing BV?
    [Methods] Rats were divided into two groups : exercise training and no training groups (Ex and Con, respectively, n=10 each). The Con group was housed in normal cages at 23℃ in LD 12:12 h. The Ex group was also housed in the same condition but had 1-h treadmill exercise for 4 weeks (5 days/week). The treadmill speed was set at 10 m/sec and the slope was increased from 0°to 15°every week. At the end of 3rd week, two catheter were placed in the right femoral and jugular veins, respectively. In 5th week plasma volume (PV) of each rat was determined by Evans Blue dye dilution technique. Hematocrit, plasma albumin and total protein and oncotic pressure were also measured, and BV and plasma albumin content were calculated. Albumin synthesis was evaluated by measuring albumin mRNA level in the liver using Nothem blotting technique.
    [Results] BV and plasma volume in the Ex group was greater (P<0.05) than in the Con group (6.4 ± 0.2 and 4.8 ± 0.5 ml/100g body weight, respectively). Plasma albumin content was also greater (P<0.05) than in the Con group (0.13 ± 0.01 and 0.10 ± 0.01 ml/100g body weight, respectively). The expression of albumin mRNA in the liver was similar in the two groups.
    [Conclusion] Four-week treadmill exercise could increase BV in rats. The increase in plasma albumin could be a mechanism for this response. Although the expression of albumin mRNA in the liver remained unchanged between the Ex and Con groups, exercise training may have facilitated albumin synthesis in the earlier period.

  • Systems for analysing thermal emotion (behavioral thermoregulation)

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B).

    Project Year :

    1999
    -
    2000
     

    KANOSUE Kazuyuki, NAGASHIMA Kei, INOUE Oamu, YAMATODANI Atsushi

     View Summary

    In the present study we developed systems for analyzing (1) thermal comfort sensation in human and (2) behavioral thermoregulation in rats. In humans we used fMRI to search the brain regions activated by whole body cooling or warming. For thermal stimulation we have developed a special bag in which a human subject laid in supine position. Temperature controlled air flow through the bag. Eight male subjects were exposed to cool air of 8 or 13℃ for 22 min and scored their thermal comfort in every 1 min. The subjective thermal comfort score was correlated with rCBF changes in bilateral amygdala ; i.e.the neural activity there increased as the subjects felt it cold. It is suggested that the amygdala plays some role in the genesis of thermal comfort and this system is considered to be useful to analyze thermal comfort sensation in human. The operant system used for the rat behavioral thermoregulation contains a box that can be convectively heated or cooled. A rat moves freely in the box. Its location is monitored photoelectrically while its deep body temperature is monitored by a telemetry system. In heat-escape experiments hot air (40℃) flows through the box. When the rat enters a reward zone the air source is switched and cold air (0℃) flows through the box for a given period (30 sec). Conversely, in cold-escape experiments cold air flows through the box and when the rat enters the reward zone the air source is switched to a warm one. Experiments show that rats quickly learn to stay near the reward zone and move in and out of it periodically. This system is based on behavior more natural than the frequently used lever-pressing response, and has many advantages for use in studies involving behavioral thermoregulation.

  • Examination of the hyposesis of thermoredulatory module in the hypothalamus.

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)

    Project Year :

    1997
    -
    1999
     

    KANOSUE Kazuyuki, NAGASHIMA Kei, INOUE Osamu, YAMATODANI Atsushi

     View Summary

    Thermosensitive neurons in the hypothalamus, especially in the preoptic area (PO), play important roles in thermoregulation (Boulant, 1980; Simon et al., 1986), but many hypotheses about signal processing in the hypothalamus have remained untested because we don't have precise enough information about the neuronal "network" there. Although it had long been almost an article of faith that warm-sensitive neurons send efferent signals driving heat loss and cold-sensitive neurons send efferent signals driving heat production, we found that warm-sensitive neurons work for the control of heat production as well as heat loss (Zhang et al., 1995). But we have obtained evidence that the PO is simply an assembly of neuronal groups, each sending its own efferent signals to each effector, and that these groups function without connections to each other (Kanosue et al., 1997). In the present study, based upon these findings, we further analyzed the neural network for thermoregulation in the hypothalamus and the midbrain. First, we analyzed the nertwork for nonshivering thermogenesis. Electrical stimulation of the ventromedial hypothalamus elicited thermogenesis of the brown adipose tissure. But the same stimulus had no effect during warming the preoptic area, which means that the PO send inhibitory signals to the VMH. Second, the role of the posterior hypothalmus was investigated by injecting inhibitory substance muscimol. Muscimol injection suppressed cold-induced shivering, which indicates that the excitatory neuron for shivering locates in the posterior hypothalamus. Third, DLH was injected into the ventropoterior region of the PAG and it facilitated nonshivering thermogenesis. The retrograde tracer CTB injection into the medullary raphe labeled neurons in the PAG where nonshivering thermogenesis was facilitated. The excitatory signal seems to decsend from the PAG to the raphe.

  • Clarification of the mechanisms involved in circadian body temperature rhythm and its physiological significance

  • Comprehensive Analysis of the mechanism involved in changes of thermoregulatory responses during dehydration

  • 夏期原発復旧除染作業・建設作業等の酷暑作業における暑熱負担軽減対策手法の開発

    科学研究費助成事業(独立行政法人労働安全衛生総合研究所)  科学研究費助成事業(基盤研究(B))

  • 温熱的快適性の形成メカニズムの解析と衣服内環境評価への応用

    科学研究費助成事業(早稲田大学)  科学研究費助成事業(基盤研究(B))

  • Examination of the hyposesis of thermoredulatory module in the hypothalamus.

  • Systems for analysing thermal emotion (behavioral thermoregulation)

  • Analysis of efferent projection from the preoptic area for the control of thermoregulatory effector responses.

  • Relationship between exercise-induced hypervolemia and albumin synthesis and distribution in the body

  • 「冷え症」の生理学的機序の研究

    科学研究費助成事業(大阪大学)  科学研究費助成事業(萌芽研究)

  • Analysis of changes in body temperature rhythm during fasting

  • Analysis of functional connection of the dorsomedial hypothalamic nucleus for themoregulation

  • 体温調節機構の性分化メカニズムの解析

    科学研究費助成事業(早稲田大学)  科学研究費助成事業(特定領域研究)

  • ランペップTM配合サプリメントの機能性に関する研究

  • 蒸気温熱及び感覚制御剤適用による感覚・認知機構の研究

  • 蒸気温熱及び感覚制御剤適用による感覚・認知機構の研究

  • 蒸気温熱及び感覚制御剤適用による感覚・認知機構の研究

  • 環境温度および清涼成分の使用による身体機能への影響に関する研究

  • 人体部位毎の温冷感感受性の研究

▼display all

Presentations

  • 水分補給と健康 PART1「なぜ水分補給は必要か」、PART2「水分補給の方法」

    永島 計

    水分補給と健康 

    Event date:
    2022.04
    -
     
  • Office syndrome-its etiology from the view of body fluid and circulation physiology

    Kei Nagsahima  [Invited]

    Understanding Office Syndrome 

    Event date:
    2022.04
    -
     
  • 暑熱馴化の決定因子の実験的考察

    永島 計、増田 雄太、加藤 一聖

    日本臨床スポーツ医学会学術集会(オンライン開催) 

    Presentation date: 2021.11

    Event date:
    2021.11
     
     
  • 暑熱順化に伴う熱産生の変化とそのメカニズムの探索

    増田雄太, 永島 計

    第60回日本生気象学会大会(オンライン) 

    Presentation date: 2021.11

    Event date:
    2021.11
     
     
  • 個別と集団の飼育条件の差がマウスの寒冷負荷時の体温調節行動に与える影響の解析―体温調節反応の可塑性の考察―

    唐木幸音, 増田雄太, 加藤一聖, 永島 計

    第60回日本生気象学会大会(オンライン) 

    Presentation date: 2021.11

    Event date:
    2021.11
     
     
  • 蒸れ感覚の形成への吸気に対する温度感覚の関与

    加藤一聖, 永島 計

    第60回日本生気象学会大会(オンライン) 

    Presentation date: 2021.11

    Event date:
    2021.11
     
     
  • マスク着用が暑熱下運動中の深部体温変化に及ぼす影響

    加藤一聖, 増田雄太, 永島 計

    第76回日本体力医学会大会 2021,9,17-19 

    Presentation date: 2021.09

    Event date:
    2021.09
     
     
  • 蒸れ感覚の要因としての鼻部温度の関与

    加藤一聖, 永島 計

    2021年度温熱生理研究会 2021,8,31 

    Presentation date: 2021.08

    Event date:
    2021.08
     
     
  • Exploring the mechanism of behavioral thermoregulation in female mice

    Yukine Karaki, Yuta Masuda, Issei Kato, Kei Nagashima

    第44回日本神経科学会大会, 2021年7月28-31日, 神戸 

    Presentation date: 2021.07

    Event date:
    2021.07
     
     
  • Study on the mechanism of humid perception generation in human

    Issei Kato, Kei Nagashima

    第44回日本神経科学会大会 2021,7,28-31 

    Presentation date: 2021.07

    Event date:
    2021.07
     
     
  • 温度と湿度感覚の神経基盤

    永島 計  [Invited]

    第58回日本リハビリテーション医学会学術集会 

    Presentation date: 2021.06

    Event date:
    2021.06
     
     
  • 熱中症の原因の理解から、適切な予防法を身につけよう!

    永島 計

    農林水産研修所 令和3年度農作業安全組織計画・運営研修(オンライン研修)「熱中症及び作業環境対策コース」 

    Presentation date: 2021.06

    Event date:
    2021.06
    -
     
  • Resting heart rates reflet thermal load during intermittent exercise

    Issei Kato, Yuta Masuda, Shuri Marui, Kei Nagashima

    Experimental Biology 2021 2021,4,27-30 

    Presentation date: 2021.04

    Event date:
    2021.04
     
     
  • "Fundamental Research on Behavioral Thermoregulation and Thermal Perception -Using a novel cross-shaped behavioral thermoregulatory system-"

    Presentation date: 2021.03

    Event date:
    2021.03
     
     
  • Effect of elevated core body temperature on heart rate recovery during exercise interruption

    Presentation date: 2021.03

    Event date:
    2021.03
     
     
  • 湿度感覚のメカニズムの探索

    小林美沙, 加藤一聖, 永島 計

    第16回日本感性工学会春季大会(オンライン開催) 

    Presentation date: 2021.03

    Event date:
    2021.03
     
     
  • 温度及び水分量がヒトの濡れ感覚知覚に及ぼす影響

    加藤一聖, 増田雄太, 丸井朱里, 永島 計

    第16回日本感性工学会春季大会(オンライン開催) 

    Presentation date: 2021.03

    Event date:
    2021.03
     
     
  • 「入浴時の温熱負荷の検討」

    増田雄太, 丸井朱里, 加藤一聖, 永島 計

    第85回日本温泉気候物理医学会総会・学術集会(東京) 

    Presentation date: 2021.01

    Event date:
    2021.01
     
     
  • 新しい十字形マウス行動性体温調節評価システムの開発とその有効性の検証

    坂井里帆, 増田雄太, 丸井朱里, 永島 計

    第59回日本生気象学会大会(奈良女子大学Web開催) 

    Presentation date: 2020.11

    Event date:
    2020.11
     
     
  • 暑熱下運動中のマスク着用が体温調節へ及ぼす影響

    加藤一聖, 山本晏未, 増田雄太, 丸井朱里, 永島 計

    第59回日本生気象学会大会 奈良女子大学(Web開催) 

    Presentation date: 2020.11

    Event date:
    2020.11
     
     
  • "加湿冷却装置の生理学的冷却効果の検討"

    増田雄太, 加藤一聖, 丸井朱里, 唐木幸音, 永島 計

    第57回生気象学会大会(オンライン) 

    Presentation date: 2020.11

    Event date:
    2020.11
     
     
  • "Thermal perception of heat may be suppressed during heat acclimation, which augments spontaneous exercise in mice"

    Presentation date: 2020.10

    Event date:
    2020.10
     
     
  • 運動中のマスク着用の有無が深部体温および身体周囲環境に及ぼす影響

    加藤一聖, 増田雄太, 丸井朱里, 永島 計

    温熱生理研究会(Web開催) 

    Presentation date: 2020.08

    Event date:
    2020.08
    -
     
  • "カプサイシン投与による脱感作が暑熱下での自発運動抑制に及ぼす影響"

    増田雄太, 丸井朱里, 加藤一聖, 唐木幸音, 坂井里帆, 永島 計

    2020年度温熱生理研究(オンライン) 

    Presentation date: 2020.08

    Event date:
    2020.08
     
     
  • 「COVID-19による活動自粛後の活動再開時における安全・健康管理」

    増田雄太

    日本アスレティックトレーニング学会 学会員対象ウェビナー(オンライン) 

    Presentation date: 2020.05

    Event date:
    2020.05
     
     
  • "Influence of heat acclimation on spontaneous wheel-run exercise in mice"

    Experimetal Biology2020(オンライン) 

    Presentation date: 2020.04

    Event date:
    2020.04
     
     
  • Fun to study body temperature regulation:from the observation of the whole-body response

    Presentation date: 2020.03

    Event date:
    2020.03
     
     
  • Relationship between minimum heart rates and body tempreature during 2-min resting period of intermittent exercise

    Presentation date: 2020.03

    Event date:
    2020.03
     
     
  • An examination of the Factor of Heat Tolerance During Passive Heat Stress

    Presentation date: 2020.03

    Event date:
    2020.03
     
     
  • Heat tolerance,not related to heat loss responses

    Presentation date: 2020.03

    Event date:
    2020.03
     
     
  • Menstrual cycle influence on breast skin temperature measured during sleep

    Presentation date: 2020.03

    Event date:
    2020.03
    -
     
  • 熱感覚および暑熱順化がマウスの自発的運動に及ぼす影響

    増田雄太, 加藤一聖, 丸井朱里, 永島計

    第14回環境生理学プレコングレス  (兵庫) 

    Presentation date: 2019.03

  • The effect of environment temperature on spontaneous exercise in mice

    Yuta Masuda, Shuri Marui, Ken Tokizawa, Issei Kato, Kei Nagashima

    9th Federation of the Asian and Oceanian Physiological Scocieties  (Kobe, Japan) 

    Presentation date: 2019.03

  • Estimation of basal body temperature from breast skin temperature during sleep

    NAGASHIMA, Kei, Shuri Marui

    FAOPS2019  (Kobe, Japan) 

    Presentation date: 2019.03

  • 熱感覚および暑熱順化がマウスの自発的運動に及ぼす影響

    永島 計, 増田 雄太

    第46回自律神経生理研究会  (東京) 

    Presentation date: 2018.12

  • 熱中症をサイエンスする

    永島 計  [Invited]

    第5回スポーツセーフティージャパンシンポジウム 

    Presentation date: 2018.11

  • 心拍数、加速度測定による中学生における運動中の身体負荷の評価

    加藤一聖, 増田雄太, 丸井朱里, 永島計

    第57回日本生気象学会大会 

    Presentation date: 2018.10

  • 寒冷環境下における入浴時の温熱負荷の検討

    増田雄太, 丸井朱里, 加藤一聖, 藤木真由香, 中田真理子, 永島計

    第57回生気象学会大会  (京都) 

    Presentation date: 2018.10

  • フィールドにおける運動時の熱中症予防を目指した生体信号データの活用

    加藤一聖

    統計数理研究所共同研究集会 

    Presentation date: 2018.10

  • Ear thermometer for heat illness prevention

    NAGASHIMA, Kei  [Invited]

    BioJapan2018 

    Presentation date: 2018.10

  • Development of a 5-day heat acclimation protocol with fan cooling during a resting interval and possible factors involved in the mechanism.

    Yuta Masuda, Norikazu Hirose, Kei Nagashima

    7th International Conference on the Physiology and Pharmacology of Temperature Regulation  (Split, Croatia) 

    Presentation date: 2018.10

  • 就寝中の胸部皮膚温度を用いた基礎体温の推定に関する研究

    永島 計, 丸井 朱里

    第57回日本生気象学会大会  (京都) 

    Presentation date: 2018.10

  • The influence of estradiol on heat tolerance in female rats and its circadian difference

    NAGASHIMA, Kei, Shuri Marui

    PPTR2018, Split  (Croatia) 

    Presentation date: 2018.10

  • 暑熱下運動による暑熱順化獲得の決定因子の探索

    増田 雄太, 広瀬統一, 永島計

    第73回日本体力医学会大会  (福井) 

    Presentation date: 2018.09

  • 中学生の運動時における身体負荷の評価ー温熱負荷を中心にー

    加藤一聖

    第73回日本体力医学会大会 

    Presentation date: 2018.09

  • 心拍周期測定による中学生の運動時における温熱負荷の評価

    加藤一聖, 増田雄太, 丸井朱里, 永島計

    温熱生理研究会 

    Presentation date: 2018.08

  • 体表の温度入力がマウスの自発的運動に及ぼす影響

    永島 計, 増田 雄太

    温熱生理研究会  (愛知) 

    Presentation date: 2018.08

  • 局所寒冷刺激時の皮膚血管反応と体温変動に及ぼす肥満度の影響

    永島 計, 丸井 朱里

    平成30年度温熱生理研究会  (岡崎) 

    Presentation date: 2018.08

  • Evaluation of a novel short term heat acclimation protocol using fan cooling.

    Yuta Masuda, Sadayoshi Sakai, Kei Nagashima, Norikazu Hirose

    The 23rd Annual Congress of the European College of Sport Science  (Dublin, Ireland) 

    Presentation date: 2018.07

  • Reduction of plasma estradiol affects heat tolerance in female rats and its circadian difference

    NAGASHIMA, Kei, Shuri Marui

    Menopause2018  (Vancouver, Canada) 

    Presentation date: 2018.06

  • Daily exercise may aleter anti-oxydative responses to heat in mouse brain

    Presentation date: 2015.03

  • Reduction of plasma estradiol level affects daily rhythms of body core and tail skin temperature in female rats

    Presentation date: 2015.03

  • 温度刺激誘発性前頭葉脳活動の近赤外スペクトロスコピーを用いた評価

    Presentation date: 2015.03

  • Oxidative stress and anti-oxidative responses during hyperthermia in mice brain and the effect of daily exercise

    Presentation date: 2015.03

  • 暑熱暴露時の脳での酸化、抗酸化反応、褐色脂肪組織の反応から見た熱中症の発生メカニズムの探索と運動による予防効果

    Presentation date: 2015.03

  • NIRSを用いた温度刺激誘発性前頭葉神経活動の評価

    Presentation date: 2015.03

  • Deprivation of plasma estradiol level affects circadian rhythms of body core and tail skin temperature in ovariectomized rats

    Presentation date: 2015.03

  • NIRSによる人の温熱的感覚、温熱的快適性のメカニズムの探索

    Presentation date: 2015.02

  • 温熱的快適感のメカニズムの探索-動物の行動実験と人の感覚実験からの知見

    Presentation date: 2015.02

  • 高体温時のマウスの脳における酸化・抗酸化反応と日常運動による影響

    Presentation date: 2014.12

  • 環境温、高脂肪高カロリー食が体温と睡眠覚醒リズムに及ぼす影響

    Presentation date: 2014.10

  • NIRSを用いた温熱的快適感の評価

    Presentation date: 2014.10

  • 血中エストラジオール濃度の消褪が雌ラットの尾部皮膚温日内リズムに及ぼす影響

    Presentation date: 2014.10

  • 暑熱暴露時の脳での酸化、抗酸化反応、褐色脂肪組織の反応から見た熱中症の発生メカニズムの探索と運動による予防効果

    Presentation date: 2014.10

  • The hypothalamus is not involved in cause of heat stroke

    Presentation date: 2014.09

  • The influence of estrogen on circadian rhythms of heart rates and cardiac sympathetic nerve in female rats

    Presentation date: 2014.04

  • 摂食条件および寒冷環境が体温と睡眠覚醒リズムの同期性に与える影響

    Presentation date: 2014.03

  • The effect of the head or neck cooling on body core temperature and thermal pleasantness in humans

    Presentation date: 2014.03

  • NIRSを用いた温度感覚の評価

    Presentation date: 2014.03

  • 環境温、摂食条件が睡眠に与える影響

    Presentation date: 2014.02

  • 絶食、寒冷暴露が体温と睡眠覚醒リズムの同期性に及ぼす影響

    Presentation date: 2013.12

  • 頭頸部冷却が深部体温に及ぼす影響

    Presentation date: 2013.11

  • NIRSを用いた温度感覚の評価

    Presentation date: 2013.11

  • マウス非活動期に見られる体温のウルトラディアンリズムは睡眠覚醒リズムに追随する

    Presentation date: 2013.11

  • Ultradian rhythm of bodytemperature and its relation to wake-sleep rhythm

    Presentation date: 2013.09

  • The effect of head or neck cooling on body core temperature

    Presentation date: 2013.09

  • The effect of estrogen on blood pressure fluctuation in female rats

    Presentation date: 2013.09

  • Blood osmolality modulates skin blood flow and thermoregulation: from human to animal study and the central to periphery

    Presentation date: 2013.08

  • Blood osmolality modulates skin blood flow and thermoregulation: from human to animal study and the central to periphery

    Presentation date: 2013.08

  • Exercise training activates behavioral thermoregulation in mice

    Presentation date: 2013.07

  • The influence of estrogen on circadian rhythms of body temperature and heart rates in female rats

    Presentation date: 2013.07

  • The effect of spontaneous running wheel exercise on behavioral thermoregulation in heat and thermal preference in mice:a possible role of the central

    Presentation date: 2013.03

  • The impact of estrogen on thermoregulatory response to the cold in female rats

    Presentation date: 2008.07

  • Effect of hypothalamic application of 17-B estradiol on thermoregulation in a cold environment

    Presentation date: 2008.07

  • Fasting affects thermoregulation and hypothalamic Fos expressions in the cold,depending on time of the day

    Presentation date: 2008.07

  • Effect of central application of 17-B estradiol on thermoregulation during a cold exposure in female rats

    Presentation date: 2008.04

  • The influence of exercise-induced dehydration on thermal sensation and comfort

    Presentation date: 2008.04

  • Fasting affects thermoregulation in the cold,depending on time of the day

    Presentation date: 2008.04

  • Estrus cycle modulates thermoregulatory responses to the cold in rats

    Presentation date: 2008.03

  • Difference in thermoregulation between dark and light phase of fasted mice

    Presentation date: 2008.03

  • Effect of hypothalamic application of 17- {beta}-estradiol on thermoregulation during a cold exposure in female rats

    Presentation date: 2008.03

  • The regional characteristics of temperature-related sensations.

    Presentation date: 2008.03

  • The influence of exercise induced dehydration on thermal sensation and comfort

    Presentation date: 2008.03

  • 温度感覚、温熱的快不快感とその研究

    Presentation date: 2008.01

  • 温度感覚、温熱的快不快感とその研究

    Presentation date: 2007.07

▼display all

Specific Research

  • 湿度が与える感性、生理への影響の探索

    2018   中田大貴, 丸井朱里, 増田雄太, 加藤一聖, 眞野博彰

     View Summary

    “湿度”や“皮膚表面の濡れ”は、ヒトの体温調節に大きな影響を与える因子であり、衣服素材の開発や室内環境設計などの応用面からも重要な研究対象である.湿度や皮膚表面の濡れは、“蒸れ感”、“濡れ感”として我々の意識にのぼり、温熱的な不快感につながる.また、物理的に発汗(蒸散性熱放散)の効率を低下させ、汗腺での汗の生成を抑制する.しかし、ヒトの濡れセンサーは同定されていない.さらに、皮膚表面の濡れの受容を起点とした体温調節反応のメカニズムは未知である.研究では、行動実験と機能的脳画像法(fMRI)を用いて、①濡れ感に関わる脳部位を同定し、②温冷感と圧覚の2つの異なる感覚から形成されると推察される濡れ感を、脳内情報伝達解析により明らかにすることを目指した。本年度は、①②に関わる行動実験を行い、MRIを用いた実験の基礎的研究を行った。また、以前から行っていた関連する温熱感覚に関わるMRI実験を完成し、論文化した。2019年度以後の基盤研究Aに本課題が採択され、研究の発展が可能となった。

  • ウエアラブルセンサーを用いた集団生理学の確立

    2017  

     View Summary

    運動時の安全、快適性、パフォーマンス、トレーニング効果の点から、個体間の環境応答の差異を研究対象とすることを目指し、生理学データの集団測定を行う基本技術を確立することを目指した.被験者は高校ハンドボール選手5名を対象に5日の異なる日に実験を行なった。グランドでの運動時に1台のスマートフォンを保持し、ウエアラブルセンサーを用いて耳道温度、心拍数(MIOLINK)を連続測定し、bluetoothにてデータ採取した. 3D加速度センサー(OMORON•HJA-750C)を保持し、体重をもとに運動時の仕事量を連続測定した.フィールドにおいても生理学的パラメータの測定が可能であることが明らかになった。

  • 脳内酸化・抗酸化バランスからみた熱中症発症機転の解明

    2016   小幡千紗, 綱川みづき

     View Summary

    平常体温のマウスの脳において、抗酸化酵素であるsuperoxide dismutase (SOD) 2のタンパク発現が脳表部で脳底部に比べて多いことを明らかにしている(p=0.001;脳表部&nbsp;1.3±0.11、脳底部&nbsp;0.3±0.05)。さらに、暑熱暴露後のSOD2のタンパク発現を調べたところ、脳表部では正常体温に比べて減少していたが(p=0.001;正常体温時&nbsp;1.3±0.11、高体温時&nbsp;0.8±0.05)、脳底部では差が見られなかった(p&gt;0.05;正常体温時&nbsp;0.3±0.05、高体温時&nbsp;0.3±0.03)。この現象の生理学的意義は明らかではない。&nbsp;本研究では以下の実験仮説をたて、その検証を行った。①暑熱暴露時には脳表部の温度が上がりやすく、その防御機転の一つとして抗酸化酵素が細胞中に多く含まれている、②脳底部では暑熱暴露時に脳温の上昇を防ぐなんらかのメカニズムが存在し、抗酸化酵素は少なく、暑熱暴露の影響も小さい。実験では、高体温時のラットの視床下部および海馬の温度を直接計測し、免疫組織化学的手法により抗酸化酵素およびROSの分布を調べた。&nbsp;傾向としてはSOD2の発現は免疫組織科学染色では脳表に多く認められ、また暑熱暴露時のROS産生は多い傾向が見られた。また、脳内での温度の較差が認められた。

  • ラット暑熱暴露時の体幹深部、脳内温度較差の評価

    2015   綱川みずき, 丸井朱里

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    研究では熱中症の発症機序として、脳内の温度上昇、また高温による酸化ストレスの亢進が生じているのではないかと仮定し、実験を進めた。暑熱暴露したラットの脳温、特に視床下部の温度は、他の体深部の温度より低く、何んらかの保護機構があるのではないかと考えられた。しかし、酸化ストレスは脳内で変化はなく、高体温は脳内のいずれの部位でも一様に、酸化ストレスを与える可能性が示された。

  • 脳視床下部の酸化ストレス/抗酸化反応からみた熱中症発症、暑熱耐性獲得機序の解明

    2014   丸井朱里, 綱川みずき, 小幡千紗

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    &nbsp;熱中症の最も根源的な問題は、体温調節能が破綻することにある。特に体温調節中枢である視床下部の機能の破綻が予想されているが、明確な証拠は提示されていない。本研究ではマウスが暑熱暴露された際に、視床下部で高体温による障害が生じやすくなるのか/あるいは障害を防ぐ機序が存在するのかを酸化/抗酸化反応のバランスから評価を試みた。また、暑熱耐性が生じると言われる運動の効果を評価した。

  • 時間ー体温ー摂食を統合する視床下部神経ネットワークの解析

    2010   時澤、健

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    我々は絶食によって寒冷時の体温調節反応が弱められることを報告している(Tokizawa et al. Neuroscience 2009)。その反応は、マウスにおいて暗期と比べて明期(非活動期)に特に大きく弱められる。この時間特異性のメカニズムとして、時計遺伝子Clockおよび視交叉上核の神経活動亢進に伴う室傍核の活動抑制が関与することを我々は明らかにしている。しかし、絶食によって引き起こされるどのような因子が体温調節反応を減弱させるのか、また時間特異的な反応を引き起こすのかは明らかではない。そこで本研究では、絶食によって変化する摂食ペプチド(レプチンの低下、グレリンの増加)が時間特異的な体温調節反応に関与しているか否かを検証することを目的として行った。【2010年度 進捗状況報告】実験1:野生型およびレプチンを欠損するob/obマウスを、27℃の環境温で12h-12hの明暗サイクル(午前7時(ZT0)点灯,午後7時(ZT12)消灯)で飼育した。体温および活動量の概日リズムが確認された後、20℃の寒冷暴露を明期(ZT1~4)または暗期(ZT13~16)に行った。実験2:野生型マウスにおいて、腹腔内にグレリン(8 nmol)をZT1またはZT13に投与し、10℃の寒冷暴露を明期(ZT2~4)または暗期(ZT14~16)に行った。対照として、生理食塩水を投与する試行も行った。両実験において、寒冷暴露時の深部体温と活動量をテレメトリー、酸素摂取量を間接的カロリメトリーにてそれぞれ測定した。また寒冷暴露直後に脳を採取し、神経活動のマーカーであるc-Fos蛋白の免疫組織化学染色を行った。【結果】実験1:野生型マウスにおいては明期と暗期ともに、寒冷暴露によって深部体温は変化しなかった。また酸素摂取量は有意に増加した。ob/obマウスにおいて、寒冷暴露により深部体温は有意に低下した。明期と暗期の間で有意な差は認められなかった(明期、3.8 ± 0.8℃;暗期2.1 ± 0.3℃)。寒冷暴露によって酸素摂取量は増加したものの、野生型と比較して有意に低かった。c-Fos蛋白の免疫陽性細胞数は、視床下部のいずれの神経核においても野生型とob/obマウスの間で有意な差は認められなかった。実験2:グレリンを投与した野生型マウスにおいて、明期の寒冷暴露によって深部体温は生理食塩水投与と比較して有意に低下した。また酸素摂取量は増加したものの、生理食塩水投与と比較して有意に低かった。一方暗期においては、グレリン投与試行の寒冷暴露によって深部体温は低下せず酸素摂取量は有意に増加し、生理食塩水投与との間に有意な差は認められなかった。c-Fos蛋白の免疫陽性細胞数は、視交叉上核において明期にグレリン投与によって有意に増加した。また弓状核においては、明期と暗期ともにグレリン投与によってc-Fos蛋白の免疫陽性細胞数は増加したものの、暗期の方で増加は大きかった。室傍核において、グレリン投与のみではc-Fos発現は見られなかったものの、グレリン投与で寒冷暴露を行った暗期においては、c-Fos蛋白の免疫陽性細胞数は有意に増加した。【結論】レプチンの欠損は体温調節反応を弱めるが、絶食時に見られる時間特異的な体温調節反応の減弱には関与しないことが示唆された。一方グレリンの増加は、時間特異的に明期にのみ体温調節反応を弱める可能性が示唆され、絶食時に見られる時間特異的な体温調節に関与する可能性が考えられた。

  • 生物時計と体温概日リズム形成の間のシグナルパスウエイの解明

    2009  

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    【目的】本研究は1)絶食が体温調節反応に及ぼす影響を暗期(活動期)と明期(非活動期)に分けて解析し,体温調節にかかわる脳視床下部での神経核、生物リズムの形成にかかわっていると考えられている時計遺伝子の一つであるClockとの関与を明らかにすること、2)低体温を引き起こすシグナルとしてグレリンの関与を調べた.【方法】実験1:マウスの体温と活動量を連続測定し,2日間の絶食を行った.絶食開始時刻は午前9時もしくは午後9時とし,各々この時間の47時間後,午前8時(明期)と午後8時(暗期)に,20&#186;Cの寒冷暴露を行った.脳のcFosタンパクの免疫組織化学染色を行った.時計遺伝子Clockの変異マウスを用いて,同様の実験を行った.実験2:野生型マウスにグレリン(8 nmol)または生食を午前8時(明期)と午後8時(暗期)投与し、10℃の寒冷暴露を行った.【結果】実験1:絶食明期寒冷暴露時には,体温は30分目以降低下したが酸素摂取量に変化はなかった.絶食暗期寒冷暴露時には,130分目以降低下し,酸素摂取量は増加した.体温の低下は明期に暗期と比較して大きく,酸素摂取量の増加は暗期が明期に大きかった.視床下部のcFos免疫陽性細胞数は,内側視索前野および室傍核で暗期寒冷暴露に増加した.視交叉上核では,絶食、寒冷暴露によりcFosは増加した.Clock変異マウスでは,明期と暗期の間に差はみられなかった.実験2:グレリンを投与したマウスにおいて、明期の寒冷暴露により体温は低下した.酸素摂取量は低かった.暗期では、グレリン投与後の寒冷暴露により体温は低下せず酸素摂取量は増加した.cFos-は視交叉上核において明期にグレリン投与により増加した.【考察】1.摂食条件は体温調節に大きく関わっており,絶食時には寒冷時の体温調節反応を時間特異的に抑制することが明らかになった.時間特異的に抑制するメカニズムに時計遺伝子Clockが関与していることが明らかになった. 2.絶食時の低体温は調節された現象と考えられるが、絶食に伴い増加するグレリンは絶食時の時間特異的な体温調節に関与していると考えられる。

  • 体温の運動パフォーマンスに与える影響の解析ー温熱的快不快感を尺度としてー

    2007   時澤 健

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    [目的]運動に伴う脱水は、発汗&#8226;皮膚血管拡張などの自律性体温調節反応の抑制を引き起こし、体温調節能の低下とともに、熱中症などにつながる。自律性体温調節とともに人では衣服の着脱、エアコンのオン&#8226;オフなどの行動性体温調節があり、自律性体温調節に対して相補的に働くと言われている。しかしながら、脱水時に行動性体温調節がいかに変化するかは未知のままである。今回の研究では、脱水時の行動性体温調節がいかに変化するのかを、人の行動性体温調節の動機である温度感覚&#8226;温熱的快不快感を指標に調べた。[方法]健康成人男子(22-28 y.o.)を対象にした。実験は2つの試行を1週間以上の間隔をおいて行った。1つめの試行は24°Cの環境下に~10%HRmaxの運動を、2つめの試行は35°Cの環境下に40%HRmaxの運動を各々40分間行った。その後27°Cから22°Cの環境まで、次に27°Cから38°Cの環境まで変化させこの間、深部体温、表面皮膚温、発汗率、皮膚血流、温度感覚、温熱的快不快感を測定し、運動前後&#8226;実験終了後の血液を採取した。[結果] 35°Cの環境下での運動では約1%体重の脱水が生じ、その後38°Cの環境で発汗、皮膚血流量の抑制が、24°Cの環境下での運動後の値に比較して見られ、深部体温も上昇した。しかしながら、温度感覚/温熱的快不快感については両者に差は見られなかった。[結論]暑熱下運動による軽度の脱水後にも、暑熱環境に暴露させると発汗、皮膚血流などの自律性体温調節反応が低下し、深部体温の上昇が見られた。しかしながら、温度感覚/温熱的快不快感には差が認められず、人においては自律性体温調節に対して行動性体温調節の動機となると予想される温度感覚/温熱的快不快感が相補的に亢進する証拠は認められなかった。

  • 体温調節機構としての視交叉上核の役割の解明

    2004  

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    緒言 恒温動物には体温を一定に保つための優れたシステムが存在するが一方体温には明確な概日リズムが存在することが知られている。これは身体活動や代謝の活動により二次的に生じたものではなく積極的に調節されるものであることが申請者らの研究により明らかになってきている。目的 研究では生物時計の最上位中枢と考えられている脳視床下部の視交差上核がいかに体温調節反応を変化させ体温のリズムを形成しているかを明らかにしていくことを目的とした。実験1 ラットの視交叉上核を電気的に破壊し、十分な回復の後、高温(33度)、低温(18度)に暴露させこの際の体温調節を調べた。次に体内の熱産生を低下させる強い刺激である絶食をおこなった上で同様に体温の変動を測定した。結果 視交叉上核の破壊により体温のリズムは消失するが、いずれの環境温、摂食状態においても体温は一定に保たれた。しかし非破壊ラットにおいては絶食時に体温の低下が非活動期において認められた。さらに非破壊ラットにおいては環境温度の変動に対し熱産生量を変動させ体温を調節させるのに対し、視交叉上核破壊ラットではそのような反応が減弱していた。また摂食情報の伝達には迷走神経が重要な役割を果たしていることが付随する研究で明らかになった。実験2 時計遺伝子Cryの欠損マウスを用いてその体温の変動と熱産生の変動の関係を調べた。結果 Cryの欠損マウスでは体温の変動は熱産生の変動に依存している。一方正常マウスでは熱産生の変動に対しても体温を一定に保つメカニズムが存在した。結論 視交叉上核またその活動に反映する時計遺伝子は体温調節反応の制御に関わっていると考えられた。また摂食あるいは代謝の情報を視交叉上核はうけているとかんがえられ、さらに体温調節に影響をあたえていると推測される。上記研究結果についてはAmerican Journal of Physiology、Autonomic Neuroscienceに現在投稿中である。

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    オーストラリア   ウロンゴン大学

 

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    ちょっとブレイクタイム(日本テレビ) 

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    座りっぱなし症候群についての解説

  • カオログ(日本テレビ)

    カオログ(日本テレビ) 

     View Summary

    座りっぱなし症候群についての解説

  • ②ちゃおちゃおパラダイス(テレビ朝日)

    ②ちゃおちゃおパラダイス(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • 八千代コースター(フジテレビ)

    八千代コースター(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 4U(日本テレビ)

    4U(日本テレビ) 

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    座りっぱなし症候群についての解説

  • 花咲かタイムズ(TBS)

    花咲かタイムズ(TBS) 

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    座りっぱなし症候群についての解説

  • たーげっちゅ(フジテレビ)

    たーげっちゅ(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 昼まで待てない(テレビ朝日)

    昼まで待てない(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • 日曜なもんで!(テレビ東京)

    日曜なもんで!(テレビ東京) 

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    座りっぱなし症候群についての解説

  • ほほ笑みチャンネル(フジテレビ)

    ほほ笑みチャンネル(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 情報パラダイス(テレビ朝日)

    情報パラダイス(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • ミタイノコレクション(TBS)

    ミタイノコレクション(TBS) 

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    座りっぱなし症候群についての解説

  • 北陸ビジネスサテライト(テレビ朝日)

    北陸ビジネスサテライト(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • MONOモノ倶楽部(日本テレビ)

    MONOモノ倶楽部(日本テレビ) 

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    座りっぱなし症候群についての解説

  • ネクストジャパン(TBS)

    ネクストジャパン(TBS) 

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    座りっぱなし症候群についての解説

  • ハピくるっ!(フジテレビ)

    ハピくるっ!(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 本日はダイアンなり(テレビ朝日)

    本日はダイアンなり(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • たこるの耳より情報(テレビ東京)

    たこるの耳より情報(テレビ東京) 

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    座りっぱなし症候群についての解説

  • 驚き桃の木ナオキの樹(テレビ朝日)

    驚き桃の木ナオキの樹(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • キレ☆カワ女子部(テレビ東京)

    キレ☆カワ女子部(テレビ東京) 

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    座りっぱなし症候群についての解説

  • ひるじげドン(日本テレビ)

    ひるじげドン(日本テレビ) 

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    座りっぱなし症候群についての解説

  • トク盛りGopan(フジテレビ)

    トク盛りGopan(フジテレビ) 

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    座りっぱなし症候群についての解説

  • かぼすタイム(TBS)

    かぼすタイム(TBS) 

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    座りっぱなし症候群についての解説

  • 金様の鍵(テレビ朝日)

    金様の鍵(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • タウンスナップ(フジテレビ)

    タウンスナップ(フジテレビ) 

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    座りっぱなし症候群についての解説

  • ナイトマガジン(フジテレビ)

    ナイトマガジン(フジテレビ) 

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    座りっぱなし症候群についての解説

  • サタブラ(テレビ朝日)

    サタブラ(テレビ朝日) 

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    座りっぱなし症候群についての解説

  • ゆめいろ@ネット(日本テレビ)

    ゆめいろ@ネット(日本テレビ) 

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    座りっぱなし症候群についての解説

  • 見っどナイト(フジテレビ)

    見っどナイト(フジテレビ) 

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    座りっぱなし症候群についての解説

  • パブロフ(フジテレビ)

    パブロフ(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 沖縄BON(TBS)

    沖縄BON(TBS) 

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    座りっぱなし症候群についての解説

  • ひーぷー☆ホップ(フジテレビ)

    ひーぷー☆ホップ(フジテレビ) 

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    座りっぱなし症候群についての解説

  • ゴジカル!(日本テレビ)

    ゴジカル!(日本テレビ) 

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    座りっぱなし症候群についての解説

  • じゃらん!² モーニング(TBS)

    じゃらん!&sup2; モーニング(TBS) 

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    座りっぱなし症候群についての解説

  • ハピ♡どき(フジテレビ)

    ハピ♡どき(フジテレビ) 

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    座りっぱなし症候群についての解説

  • 健康カプセル!ゲンキの時間(TBS)

    健康カプセル!ゲンキの時間(TBS) 

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    頻尿&残尿の原因&対策は?

  • めざましどようび

    めざましどようび 

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    環境温度の感覚についての解説

  • めざましテレビ

    めざましテレビ 

     View Summary

    環境温度の感覚についての解説

▼display all

Media Coverage

  • 知っているようで知らない体温のはなし

    愛知銀行   サークルゆうゆう事務局(愛知銀行)59号  

    特集  

    2021.09

  • 体温で24時間を整える

    Newspaper, magazine

    朝日新聞出版   WEEKLY AERA  

    巻頭特集P10-19  

    2020.11

  • 「非接触型体温計」本当に正確か?

    Newspaper, magazine

    小学館   週刊ポスト  

    P131-P133  

    2020.11

  • 平熱37度の人が困る社会

    毎日新聞   毎日📰東京朝刊  

    くらしナビ-ライフスタイル-  

    2020.08

  • マスクは熱中症の敵か

    日経BP   日経コンストラクション  

    特集 マスクは熱中症の敵か  

    2020.08

  • 平熱37度以上の人が困っているコロナ社会 検温に問題はないか調べてみた

    Newspaper, magazine

    毎日新聞   毎日新聞8月12日デジタル版  

    2020.08

  • 夏を乗り切る水分補給

    朝日新聞   スタイルアサヒ8月号特集  

    2020.08

  • 検温・消毒 困っている人がいる

    朝日新聞東京本社   朝日新聞  

    7月22日朝刊32頁  

    2020.07

  • マスク・暑さに慣れぬ体 熱中症対策を

    朝日新聞東京本社   朝日新聞朝刊  

    生活  

    2020.06

  • 最後のクールビズ期間、始まる コロナで熱中症にも注意

    朝日新聞DEGITAL   朝日新聞DEGITAL  

    2020年5月1日有料会員記事  

    2020.05

  • 話題の本「40℃越えの日本列島でヒトは生きていけるのか」著者永島計氏に聞く

    Newspaper, magazine

    東洋経済新報社   週刊東洋経済  

    2019.09

  • 背表紙『40℃越えの日本列島でヒトは生きていけるのか』…暑さ、感じる前に

    Newspaper, magazine

    日本共産党中央委員会   しんぶん赤旗  

    2019.08

  • 酷暑対策 東京五輪まで1年 打開への道

    Newspaper, magazine

    日本共産党中央委員会 スポーツ部   しんぶん赤旗  

    2019.08

  • (元気にキレイに)熱中症を防ぐ 夏は上手に水分補給

    Newspaper, magazine

    朝日新聞   朝日新聞デジタル  

    2019.07

  • 体慣れぬ5月 熱中症防げ

    Newspaper, magazine

    中日新聞 朝刊社会  

    2019.05

  • 健康に欠かせない体温調節のしくみと鍛え方

    Newspaper, magazine

    第三文明社   灯台12月号  

    2018.12

  • 想定外の猛暑 訪日客を守れ

    Newspaper, magazine

    日本経済新聞   日経西部夕刊社会面  

    2018.07

  • 想定外の暑さ 訪日客を守れ

    Newspaper, magazine

    日本経済新聞   日経大阪夕刊社会面  

    2018.07

  • エコノミー症候群を引き起こすアブナイ習慣!

    Newspaper, magazine

    小学館   女性セブン  

    2018.06

  • 夏の脳生理学 夏は人を暴力的にする!

    Newspaper, magazine

    日刊ゲンダイ   日刊現代  

    2018.06

  • 冬でも脱水症?

    Promotional material

    京都府医師会   京の衣食住  

    2018.03

  • 熱中症 プール・海でも

    Newspaper, magazine

    日本経済新聞   日経夕刊  

    2015.08

  • 「冷えは万病のもと」探る

    Newspaper, magazine

    日本経済新聞   日経朝刊  

    2009.11

  • 低体温の子なぜ減らぬ

    Newspaper, magazine

    日本経済新聞   日経夕刊  

    2008.04

▼display all