2022/12/09 更新

写真a

カメオカ ジュン
亀岡 遵
所属
理工学術院 大学院情報生産システム研究科
職名
教授
プロフィール

過去20年間、主にいろいろな材料のナノとマイクロ構造の機械的、電気的、化学的、生物学的特性を理解し、それを(1)バイオセンサー、(2)マイクロ流路、(3)再生医療エンジニアリングの分野に応用することを研究してきました。直近では、安価で多項目検知が可能なマイクロ構造を持つハイドロジェルバイオセンサーの開発、およびハイドロジェルナノ構造と幹細胞を用いた再生医療を研究しています。今後、これらの技術と、機械学習、ビッグデータ通信と解析技術を統合することにより、スマートで高効率な高齢化社会に対応した医療が実現するための技術を研究開発してくことを目標とています。

学歴

  • 1999年09月
    -
    2002年08月

    コーネル大学   工学部   電子工学科 物理デバイス系博士課程  

    博士号

  • 1995年09月
    -
    1999年08月

    コーネル大学   工学部   電子工学科物理デバイス系修士課程  

    修士号

  • 1995年09月
    -
    1997年08月

    コーネル大学   原子力工学 Master of Engineering  

  • 1991年04月
    -
    1995年03月

    千葉大学   工学部  

学位

  • 2002年08月   Cornell University   Ph.D

経歴

  • 2022年09月
    -
    継続中

    早稲田大学   大学院情報生産システム研究科   教授

  • 2017年08月
    -
    継続中

    東京慈恵会医科大学   物理学教室   客員教授

  • 2017年09月
    -
    2022年08月

    テキサスA&M大学   電子工学科   教授

  • 2010年09月
    -
    2017年08月

    テキサスA&M大学   電子工学科   准教授

  • 2004年06月
    -
    2010年08月

    テキサスA&M大学   電子工学科   助教授

  • 2002年09月
    -
    2004年05月

    コーネル大学   ナノバイオテクノロジーセンター   ポストドク

▼全件表示

所属学協会

  • 2020年12月
    -
    継続中

    国際応用材料学会

  • 2005年04月
    -
    継続中

    米国材料学会

  • 2005年03月
    -
    継続中

    国際光工学会

  • 2004年03月
    -
    継続中

    米国電気電子学会

 

研究分野

  • 生体医工学

  • ナノバイオサイエンス

  • ナノマイクロシステム

研究キーワード

  • バイオセンサー

  • マイクロ流路

  • 再生医療

  • ソフトロボティクス

論文

  • Cancer Biomarker Methylmalonic Acid Detection by Molecularly Imprinted Polyaniline Paper Sensor

       2022年02月

    DOI

  • A low cost paper sensor with molecularly imprinted conductive polymer electrode

    Kameoka, J, Chen Z, Chi TY, Dincel O

    ; International association of advanced material (European Advanced Materials Congress)     46 - 47  2021年

  • RF/Microwave Broadband Characterization of Paper Substrates

    Ghiri, R. E, Dincel, O, Kameoka, J, Entesari, K

    2021 IEEE Texas Symposium on Wireless and Microwave Circuits and Systems (WMCS)     1 - 3  2021年

  • A Low-cost and Enzyme-free Glucose Paper Sensor.

    Zheyuan Chen, Ting-Yen Chi, Onder Dincel, Lin Tong, Jun Kameoka

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference   2020   4097 - 4100  2020年07月  [国際誌]

     概要を見る

    A low-cost and enzyme-free glucose paper sensor is presented as a promising alternative to glucose test strips. This paper-based glucose sensor is prepared with molecularly imprinted (MIP) polyaniline (PANI) electrode. The determination of glucose concentrations was studied by the impedance change of the paper sensor before and after the blood samples dispensing at a low frequency. A comparison of the linear and polynomial regression was applied to analyze the impedance ratio as a function of glucose concentrations. The proposed glucose paper sensor showed a limit of detection (LoD) of 1.135 mM. This novel and non-enzymatic paper sensor suggests a low-cost glucose test assay and can improve the quality of routine testing for diabetic patients.

    DOI PubMed

  • A low-cost paper glucose sensor with molecularly imprinted polyaniline electrode

    Chen, Z., Wright, C., Dincel, O., Chi, T.-Y., Kameoka, J.

    Sensors (Switzerland)   20 ( 4 )  2020年  [国際誌]

     概要を見る

    For the hundreds of millions of worldwide diabetic patients, glucose test strips are the most important and commonly used tool for monitoring blood glucose levels. Commercial test strips use glucose oxidases as recognition agents, which increases the cost and reduces the durability of test strips. To lower the cost of glucose sensors, we developed a paper-based electrical sensor with molecularly imprinted glucose recognition sites and demonstrated the determination of various glucose concentrations in bovine blood solutions. The sensing electrode is integrated with molecular recognition sites in the conductive polymer. A calibration graph as a function of glucose concentration in aqueous solution was acquired and matched with a correlation coefficient of 0.989. We also demonstrated the determination of the added glucose concentrations ranging from 2.2 to 11.1 mM in bovine blood samples with a linear correlation coefficient of 0.984. This non-enzymatic glucose sensor has the potential to reduce the health care cost of test strips as well as make glucose sensor test strips more accessible to underserved communities.

    DOI PubMed

  • Perfluorooctanesulfonic acid detection using molecularly imprinted polyaniline on a paper substrate

    Chi, T.-Y., Chen, Z., Kameoka, J.

    Sensors (Switzerland)   20 ( 24 )  2020年  [国際誌]

     概要を見る

    Perfluorinated compounds like perfluorooctanesulfonic acid (PFOS) are synthetic water pollutants and have accumulated in environments for decades, causing a serious global health issue. Conventional assays rely on liquid chromatography and mass spectroscopy that are very expensive and complicated and thus limit the large-scale monitoring of PFOS in wastewater. To achieve low-cost and accurate detection of PFOS, we designed a paper-based sensor with molecularly imprinted polyaniline electrodes that have recognition sites specific to PFOS. The calibration curve of resistivity ratios as a function of PFOS concentrations has a linear range from 1 to 100 ppt with a coefficient of determination of 0.995. The estimated limit of detection is 1.02 ppt. We also investigated attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) spectra of the surface of the polyaniline (PANI) electrodes to propose the potential recognition sites in polyaniline matrix and the detection mechanism. This electrical paper sensor with low cost and excellent sensitivity and selectivity provides the potential for large-scale monitoring of wastewater.

    DOI PubMed

  • Digital Receptor Occupancy Assay in Quantifying On- And Off-Target Binding Affinities of Therapeutic Antibodies

    Chou, C.-K., Liu, Y.-L., Chen, Y.-I., Huang, P.-J., Tsou, P.-H., Chen, C.-T., Lee, H.-H., Wang, Y.-N., Hsu, J.L., Lee, J.-F., Yankeelov, T.E., Kameoka, J., Yeh, H.-C., Hung, M.-C.

    ACS Sensors   5 ( 2 ) 296 - 302  2020年  [国際誌]

     概要を見る

    While monoclonal antibodies are the fastest-growing class of therapeutic agents, we lack a method that can directly quantify the on- and off-target binding affinities of newly developed therapeutic antibodies in crude cell lysates. As a result, some therapeutic antibody candidates could have a moderate on-target binding affinity but a high off-target binding affinity, which not only gives a reduced efficacy but triggers unwanted side effects. Here, we report a single-molecule counting method that precisely quantifies antibody-bound receptors, free receptors, and unbound antibodies in crude cell lysates, termed digital receptor occupancy assay (DRO). Compared to the traditional flow cytometry-based binding assay, DRO assay enables direct and digital quantification of the three molecular species in solution without the additional antibodies for competitive binding. When characterizing the therapeutic antibody, cetuximab, using DRO assay, we found the on-target binding ratio to be 65% and the binding constant (Kd) to be 2.4 nM, while the off-target binding causes the binding constant to decrease by 0.3 nM. Other than cultured cells, the DRO assay can be performed on tumor mouse xenograft models. Thus, DRO is a simple and highly quantitative method for cell-based antibody binding analysis which can be broadly applied to screen and validate new therapeutic antibodies.

    DOI PubMed

  • Isolation of cancer-derived extracellular vesicle subpopulations by a size-selective microfluidic platform

    Chen, Z., Yang, Y., Yamaguchi, H., Hung, M.-C., Kameoka, J.

    Biomicrofluidics   14 ( 3 ) 034113 - 034113  2020年  [国際誌]

     概要を見る

    Extracellular vesicles (EVs) play an important role in intercellular communication. Recently, there has been increasing interest in EVs as potential diagnostic biomarkers and therapeutic vehicles. However, the molecular properties and cargo information of EV subpopulations have not yet been fully investigated due to lack of reliable and reproducible EV separation technology. Current approaches have faced difficulties with efficiently isolating EVs from biofluids, especially subpopulations of small EVs. Here, we report an EV isolation method based on a size-selective microfluidic platform (ExoSMP) via nanomembrane filtration and electrophoretic force. This unique platform offers an enhanced approach to sorting a heterogeneous population of EVs based on size, with the additional advantages of being label-free and low-cost, and featuring a short processing time (<1 h), and convenient integration with downstream analysis. In this research, we used ExoSMP to demonstrate the isolation of cancer-derived small EVs (30-120 nm) with high recovery (94.2%) and reproducibility at an optimum sample flow rate. Furthermore, we investigated isolation of EV subpopulations by altering nanomembrane combinations with different pore size combinations (50 and 100 nm, 30 and 100 nm, 30 and 200 nm, and 30 and 50 nm). This ExoSMP technique can serve as a standardized EV isolation/separation tool, facilitating the clinical prospects of EVs and opening up a new avenue for future point-of-care applications in liquid biopsies.

    DOI PubMed

  • Low Cost and Piezoelectric based Soft Wave Energy Harvester

    Sina Baghbani Kordmahale, Jitae Do, Kuang-An Chang, Jun Kameoka

    MRS Advances   4 ( 15 ) 889 - 895  2019年03月

    DOI

  • Development of size-selective microfluidic platform

    Zheyuan Chen, Hirohito Yamaguchi, Jun Kameoka

    2019 41ST ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)     5661 - 5664  2019年

     概要を見る

    Exosomes are nanosized extracellular vesicles that play a significant role in cell-cell communication. Recently, there is significant interest in exosome-related fundamental research, especially subgroups of exosomes as potential biomarkers for cancer diagnosis and prognosis. In this paper, we report a new size selective isolation method via elastic lift force and nanomembrane filtration and demonstrated the liposome recovery rate of 92.5% from a mixture solution of 1 pm polystyrene beads, 100 nm liposomes and proteins as a proof of concept for exosome isolation. This single microfluidic platform offers an improved approach with short processing time (< 2 hours) and low cost, and shows potential broad applicability to cancer biomarker studies.

  • Mechanical responses of Ecoflex silicone rubber: Compressible and incompressible behaviors

    Steck, D., Qu, J., Kordmahale, S.B., Tscharnuter, D., Muliana, A., Kameoka, J.

    Journal of Applied Polymer Science   136 ( 5 )  2019年

    DOI

  • Microencapsulation of beta cells in collagen micro-disks via circular pneumatically actuated soft micro-mold (cPASMO) device

    Huang, P.-J., Qu, J., Saha, P., Muliana, A., Kameoka, J.

    Biomedical Physics and Engineering Express   5 ( 1 )  2019年

    DOI

  • Acoustic driven microbubble motor device

    Dincel, O., Ueta, T., Kameoka, J.

    Sensors and Actuators, A: Physical   295   343 - 347  2019年

     概要を見る

    We have developed an acoustic frequency driven microbubble motor (AFMO) device and achieved highspeed rotation up to 450 RPM and torque of 2.3 x 10-9 (N.m). Additionally, the bidirectional rotation of this device has been demonstrated by modulating input frequencies. This device, directly constructed from UV curable resin by a micro-3D printer, has four microscale cavities that contain micro air bubbles when immersed in water. Once external 4 kHz acoustic waves stimulate these four cavities, microbubbles are extracted and positioned at the entrances of the cavities. These four microbubbles have identical dimensions and oscillation resonant frequencies of 5.6 kHz based on theoretical calculations that can spin the AFMO device in a clockwise direction. Both the clockwise and the counterclockwise rotation of the AFMO device have observed at a 5.6 and 5.1 kHz frequency input, respectively. Published by Elsevier B.V.

    DOI

  • Three-dimensional coaxial multi-nozzle device for high-rate microsphere generation

    Jaligama, S., Kameoka, J.

    Journal of Materials Science   54 ( 22 ) 14233 - 14242  2019年

    DOI

  • Removing perfluoroalkyl acids (PFAAs) from water Using functionalized hydrogel sorbent

    Po-Jung Huang, Myung Hwangbo, Jun Kameoka, Kung-Hui Chu

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   256  2018年08月

  • Automated microfluidic system for RNA-protein analysis

    Huang, P.-J, Hwangbo, M, Kameoka, J, Chu, K.-H

    28th Anniversary World Congress on Biosensor    2018年

  • Reusable Functionalized Hydrogel Sorbents for Removing Long- and Short-Chain Perfluoroalkyl Acids (PFAAs) and GenX from Aqueous Solution

    Huang, P.-J., Hwangbo, M., Chen, Z., Liu, Y., Kameoka, J., Chu, K.-H.

    ACS Omega   3 ( 12 ) 17447 - 17455  2018年  [国際誌]

     概要を見る

    Per- and poly-fluoroalkyl substances (PFASs) are man-made chemicals that are toxic and widely detected in the environment, including drinking water sources. A cost-effective treatment process for PFASs is currently not available. We developed reusable hydrogel sorbents to remove long- and short-chain perfluoroalkyl acids and 2,3,3,3-tetrafluoro-2-(heptafluoropropoxy)propanoic acid (GenX), which is are emerging PFAS. Through fluoridation and amination of poly(ethylene glycol) diacrylate (PEGDA), the newly synthesized sorbents can sorb the five targeted PFASs (perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), perfluorobutanesulfonic acid (PFBS), and perfluorobutanoic acid (PFBA) and GenX) to different degrees from aqueous solution. Aminated PEGDA showed the highest sorption capacity for all five PFASs, particularly for PFBA and PFBS. The bifunctionalized PEGDA showed higher capacities for PFOA and PFOS, suggesting that both hydrophobic interactions and charges contribute to the sorption. Both aminated and bifunctionalized sorbents can remove GenX from water, with the highest sorption capacity of 98.7 μmol g aminated PEGDA-1 within 6 h. The absorbed PFASs on the sorbents were observed and characterized by Fourier-transform infrared spectroscopy. The spent sorbents were reusable after readily regenerated with 70% methanol contained 1% NaCl.

    DOI PubMed

  • A flow-proteometric platform for analyzing protein concentration (FAP): Proof of concept for quantification of PD-L1 protein in cells and tissues

    Chou, C.-K., Huang, P.-J., Tsou, P.-H., Wei, Y., Lee, H.-H., Wang, Y.-N., Liu, Y.-L., Shi, C., Yeh, H.-C., Kameoka, J., Hung, M.-C.

    Biosensors and Bioelectronics   117   97 - 103  2018年  [国際誌]

     概要を見る

    Protein expression level is critically related to the cell physiological function. However, current methodologies such as Western blot (WB) and Immunohistochemistry (IHC) in analyzing the protein level are rather semi-quantitative and without the information of actual protein concentration. We have developed a microfluidic technique termed a "flow-proteometric platform for analyzing protein concentration (FAP)" that can measure the concentration of a target protein in cells or tissues without the requirement of a calibration standard, e.g., the purified target molecules. To validate our method, we tested a number of control samples with known target protein concentrations and showed that the FAP measurement resulted in concentrations that well matched the actual concentrations in the control samples (coefficient of determination [R2], 0.998), demonstrating a dynamic range of concentrations from 0.13 to 130 pM of a detection for 2 min. We successfully determined a biomarker protein (for predicting the treatment response of cancer immune check-point therapy) PD-L1 concentration in cancer cell lines (HeLa PD-L1 and MDA-MB-231) and breast cancer patient tumor tissues without any prior process of sample purification and standard line construction. Therefore, FAP is a simple, faster, and reliable method to measure the protein concentration in cells and tissues, which can support the conventional methods such as WB and IHC to determine the actual protein level.

    DOI PubMed

  • Use of Osteogenically Enhanced Mesenchymal Stem Cells and Their Cell-Derived Matrices for Bone Tissue Engineering

    Sears, C, McNeill, E, Clough, B, Jaligama, S, Kameoka, J, Gregory, C, Kaunas, R

    TISSUE ENGINEERING PART A; MARY ANN LIEBERT, INC 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA   23   S29  2017年

  • Fabrication of Hydrogel-based 3D microenvironment for Wastewater Treatment

    Kameoka, J, Huang, P.-J

    International Union of Materials Research Societies-ICA    2017年

  • Pneumatically Actuated Soft Micro-mold (PASMO)device for tissue engineering

    Kameoka, J, Huang, P.-J

    International Union of Materials Research Societies-ICA     101  2017年

  • Nanofluidic Strategies for Cancer Research

    Wang, M., Huang, P.-J., Kameoka, J., Chou, C.-K., Tsou, P.-H., Yamaguchi, H., Hung, M.-C.

    RSC Nanoscience and Nanotechnology   2017-January ( 41 )  2017年

    DOI

  • Pneumatically Actuated Soft Micromold Device for Fabricating Collagen and Matrigel Microparticles

    Huang, P.-J., Chou, C.-K., Chen, C.-T., Yamaguchi, H., Qu, J., Muliana, A., Hung, M.-C., Kameoka, J.

    Soft Robotics   4 ( 4 ) 390 - 399  2017年  [国際誌]

     概要を見る

    Collagen microparticles have recently gained more attention as viable cell confinement blocks in many biomedical research fields. Small volume and high surface area of collagen structure improve cell confinement, viability, and proliferation. Moreover, dense collagen fiber structure can protect cells from immune destruction. The ability to produce collagen microparticles in an accurate and reliable way is of upmost importance to the advancement of many biomedical researches, especially cancer research and tissue engineering. Currently, no such fabrication technique exists due to inherent fragility of collagen. Herein, we report the very first platform, pneumatically actuated soft micromold (PASMO) device, which addresses challenges in collagen microparticle production. Our new platform uses a soft micromold with a pneumatic actuator that can produce arbitrary shapes of collagen microstructures precisely from 100 μm to over 2 mm in range and can encapsulate cells inside without damaging the shape. The duplication accuracy of more than 96% in dimensions and 90% in depth has been demonstrated. The density of collagen fiber distribution is determined to be 86.57%, which is higher than that of collagen microparticles produced by other methods. We have confirmed cell viability in collagen microparticles. We also produce Matrigel™ particles as tool to develop a xenograft cancer model. The results demonstrate that Matrigel particles created by the PASMO device can reduce cell scattering for the xenograft model and the uniformity of tumors developed in mice is 12-fold improved, which can lead to an increased accuracy of cancer metastasis studies and drug screening research. These breakthroughs in the production of modular microparticles will push the boundaries of cancer research in the near future.

    DOI PubMed

  • A Metamaterial-Inspired Wideband Microwave Interferometry Sensor for Dielectric Spectroscopy of Liquid Chemicals

    Pourghorban Saghati, A., Batra, J.S., Kameoka, J., Entesari, K.

    IEEE Transactions on Microwave Theory and Techniques   65 ( 7 ) 2558 - 2571  2017年

     概要を見る

    A miniaturized wideband interferometry-based sensor for complex dielectric spectroscopy of liquid chemicals is presented in this paper. Two composite right-/left-hand (CRLH) transmission lines (TLs) are employed in a direct downconversion architecture. The material under test (MUT) is placed in direct contact with the printed interdigital capacitor of the artificial TL as the sensing component. Since the employed CRLH TL provides nonlinear dispersion characteristics, improved sensitivity with wideband (4.2-8 GHz) operation is achieved compared with resonator-or TL-based interferometers. The sensor circuit board is realized based on common printed circuit board technology, and the fluidic polydimethylsiloxane channels are fabricated and bonded to the circuit board using 3-D printing and soft lithography techniques. The measured results of pure liquid chemical characterization suggest the mean squared errors of similar to 1.1% and similar to 1.6% for epsilon(r)' and epsilon(r)", respectively, compared with the MUT's theoretical model. Moreover, binary mixture characterization is carried out based on epsilon(r)' measurements with an accuracy of 1%.

    DOI

  • A magneto-fluidic nanoparticle trapping platform for surface-enhanced Raman spectroscopy

    Huang, P.-J., Marks, H.L., Coté, G.L., Kameoka, J.

    Biomicrofluidics   11 ( 3 ) 034116 - 034116  2017年  [国際誌]

     概要を見る

    A microfluidic device utilizing magnetically activated nickel (Ni) micropads has been developed for controlled localization of plasmonic core-shell magnetic nanoparticles, specifically for surface enhanced Raman spectroscopy (SERS) applications. Magnetic microfluidics allows for automated washing steps, provides a means for easy reagent packaging, allows for chip reusability, and can even be used to facilitate on-chip mixing and filtration towards full automation of biological sample processing and analysis. Milliliter volumes of gold-coated 175-nm silica encapsulated iron oxide nanoparticles were pumped into a microchannel and allowed to magnetically concentrate down into 7.5 nl volumes over nano-thick lithographically defined Ni micropads. This controlled aggregation of core-shell magnetic nanoparticles by an externally applied magnetic field not only enhances the SERS detection limit within the newly defined nanowells but also generates a more uniform (∼92%) distribution of the SERS signal when compared to random mechanical aggregation. The microfluidic flow rate and the direction and strength of the magnetic field determined the overall capture efficiency of the magneto-fluidic nanoparticle trapping platform. It was found that a 5 μl/min flow rate using an attractive magnetic field provided by 1 × 2 cm neodymium permanent magnets could capture over 90% of the magnetic core-shell nanoparticles across five Ni micropads. It was also observed that the intensity of the SERS signal for this setup was 10-fold higher than any other flow rate and magnetic field configurations tested. The magnetic concentration of the ferric core-shell nanoparticles causes the SERS signal to reach the steady state within 30 min can be reversed by simply removing the chip from the magnet housing and sonicating the retained particles from the outlet channel. Additionally, each magneto-fluidic can be reused without noticeable damage to the micropads up to three times.

    DOI PubMed

  • Development of an Optofluidic SERS-based Biomedical Sensor

    Brian Walton, Po-Jung Huang, Jun Kameoka, Nicolaas Deutz, Gerard L. Cote

    OPTICAL DIAGNOSTICS AND SENSING XVI: TOWARD POINT-OF-CARE DIAGNOSTICS   9715  2016年

     概要を見る

    Rapid assessment of radiation exposure to sensitive organs like the gut is extremely important for large populations exposed to ionized radiation, for instance during warfare. Recent results have shown that plasma citrulline levels appear to track gut function after irradiation levels in mice and humans. The current ways to monitor blood citrulline levels are bulky, laborious, time-consuming and expensive methods. Therefore, an optofludic point-of-care (POC) system using surface enhanced Raman spectroscopy to measure plasma citrulline as a marker for radiation exposure that overcomes the above issues is being developed.As a first step toward development of this system four colloidal nanoparticles, spherical gold, silver cubes, silica-gold nanoshells, and silver-gold nanocages have been analyzed for use in the POC system. Transmission electron microscopy (TEM) images have been taken of each nanoparticle to visualize the morphology of the nanoparticles, which is vital for SERS. Ultraviolet-visible (UV/Vis) spectroscopy was also collected to verify the extinction spectra for each nanoparticle was in resonance with the excitation wavelength. The nanoparticles were functionalized with mercaptobenzoic acid (MBA), a Raman reporter molecule, and SERS spectra were collected to determine which has better utility in a novel micro-to-nanochannel. The data showed that the silver nanocubes have a larger enhancement factor than the gold nanospheres, nanoshells, or nanocages. Currently, these nanocubes are being functionalized with the citulline for assessing the concentration sensitivity and dynamic range for ultimate use as a marker for radiation.

    DOI

  • Development of automated high throughput single molecular microfluidic detection platform for signal transduction analysis

    Po-Jung Huang, Sina Baghbani Kordmahale, Chao-Kai Chou, Hirohito Yamaguchi, Mien-Chie Hung, Jun Kameoka

    MICROFLUIDICS, BIOMEMS, AND MEDICAL MICROSYSTEMS XIV   9705  2016年

     概要を見る

    Signal transductions including multiple protein post-translational modifications (PTM), protein-protein interactions (PPI), and protein-nucleic acid interaction (PNI) play critical roles for cell proliferation and differentiation that are directly related to the cancer biology. Traditional methods, like mass spectrometry, immunoprecipitation, fluorescence resonance energy transfer, and fluorescence correlation spectroscopy require a large amount of sample and long processing time. "microchannel for multiple-parameter analysis of proteins in single-complex (mMAPS)" we proposed can reduce the process time and sample volume because this system is composed by microfluidic channels, fluorescence microscopy, and computerized data analysis. In this paper, we will present an automated mMAPS including integrated microfluidic device, automated stage and electrical relay for high-throughput clinical screening. Based on this result, we estimated that this automated detection system will be able to screen approximately 150 patient samples in a 24-hour period, providing a practical application to analyze tissue samples in a clinical setting.

    DOI

  • Novel 3D coaxial flow-focusing nozzle device for the production of monodispersed collagen microspheres

    Sravani Jaligama, Po-Jung Huang, Jun Kameoka

    2016 38TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC)     4220 - 4223  2016年

     概要を見る

    We have developed a 3D coaxial flow-focusing nozzle device for the mass production of monodispersed collagen microspheres and chemically crosslinked them using EDC (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) and N-hydroxysuccinimide (NHS). The size of the microspheres was varied between 200 mu m and 600 mu m by adjusting the ratio of the flow rates of the dispersed and continuous phases. MDA231-GFP cells were attached to the surface of these particles and their viability was investigated. Because they are comprised of a natural biomaterial, these collagen microspheres will have numerous applications, including bone regeneration scaffolds for tissue engineering and analyses of cancer cell interactions in a 3D environment.

  • Comparison of Fe2O3 and Fe2CoO4 core-shell plasmonic nanoparticles for aptamer mediated SERS assays

    Haley Marks, Samuel Mabbott, Po-Jung Huang, George W. Jackson, Jun Kameoka, Duncan Graham, Gerard L. Cote

    COLLOIDAL NANOPARTICLES FOR BIOMEDICAL APPLICATIONS XI   9722  2016年

     概要を見る

    Conjugation of oligonucleotides or aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound bundles of nanoparticles which cause a measurable change in the colloid's optical properties. Here, we present further optimization of a "SERS off" competitive binding assay utilizing plasmonic and magnetic nanoparticles for the detection of the toxin bisphenol A (BPA). The assay involves 1) a 'target' silver nanoparticle functionalized with a Raman reporter dye and PEGylated BPA-binding DNA aptamers, and 2) a version of the toxin BPA, bisphenol A diglycidyl ether (BADGE), PEGylated and immobilized onto a silver coated magnetic 'probe' nanoparticle. When mixed, these target and probe nanoparticles cluster into magnetic dimers and trimers and an enhancement in their SERS spectra is observed. Upon introduction of free BPA in its native form, target AgNPs are competitively freed; reversing the nanoparticle assembly and causing the SERS signal to "turn-off" and decrease in response to the competitive binding event. The assay particles were housed inside two types of optofluidic chips containing magnetically active nickel pads, in either a straight or spotted pattern, and both Fe2O3 and Fe2CoO4 were compared as magnetic cores for the silver coated probe nanoparticle. We found that the Ag@ Fe2O3 particles were, on average, more uniform in size and more stable than Ag@ Fe2CoO4, while the addition of cobalt significantly improved the collection time of particles within the magnetic chips. Using 3D Raman mapping, we found that the straight channel design with the Ag@ Fe2O3 particles provided the most uniform nanoparticle organization, while the spotted channel design with Ag@ Fe2CoO4 demonstrated a larger SERS enhancement, and thus a lower limit of detection.

    DOI

  • Reconfigurable Quarter-Mode SIW Antenna Employing a Fluidically Switchable Via

    Ali Pourghorban Saghati, Sina Baghbani Kordmahale, Alireza Pourghroban Saghati, Jun Kameoka, Kamran Entesari

    2016 IEEE ANTENNAS AND PROPAGATION SOCIETY INTERNATIONAL SYMPOSIUM     845 - 846  2016年

     概要を見る

    A new method to fluidically switch through via posts is proposed for the first time. The reconfigurable via post is used toward designing a switchable QMSIW antenna. The switching method is based on filling/emptying a non-plated via hole with/from a liquid metal alloy. A QMSIW antenna is designed to initially work at similar to 3.2 GHz. Connecting the fluidically switchable via post at the corner of the QMSIW antenna shifts up the operating frequency. This translates into a switching range of 3.2-4.7 GHz. The Polydimethylsiloxane (PDMS) structures including the micro-channels are bonded to the QMSIW circuit board using a unique fabrication technique.

  • A Metamaterial-inspired Miniaturized Wide-band Microwave Interferometry Sensor for liquid Chemical Detection

    Ali Pourghorban Saghati, Jaskirat Singh Batra, Jun Kameoka, Kamran Entesari

    2016 IEEE MTT-S INTERNATIONAL MICROWAVE SYMPOSIUM (IMS)    2016年

     概要を見る

    This paper presents a miniature wide-band interferometry sensor for dielectric spectroscopy and detection of liquid chemicals based on utilizing two composite right/lefthanded (CRLH) transmission lines (TLs) in a zero-IF mixing configuration. The equivalent series capacitance of the CRLH TLs, constructed by using microstrip interdigital capacitors, is loaded with microfluidic channels, and exposed to the material under test (MUT) to act as the sensing element. Due to the nonlinear dispersion relation of the artificial TLs with respect to the sensing capacitor, higher sensitivity over a frequency band as wide as 4-8 GHz is achieved, compared to the previously-reported resonator-or capacitor-based sensors. The final fabricated system prototype is 4 cmx8 cm. Moreover, a calibration method is presented based on measurement results, which shows an rms error less than similar to 1.5% for liquid-chemical permittivity detection. To the best of author's knowledge, this is the first disclosure of wide-band and highly-sensitive microwave interferometry sensor suitable for portable lab-on-board applications.

  • A Microfluidically Reconfigurable Dual-Band Slot Antenna with a Frequency Coverage Ratio of 3:1

    Pourghorban Saghati, A., Singh Batra, J., Kameoka, J., Entesari, K.

    IEEE Antennas and Wireless Propagation Letters   15   122 - 125  2016年

     概要を見る

    Reconfiguration of filled and empty microchannels is used to realize a switchable dual-band slot antenna whose both bands can be controlled independently. Reactive loading effect of Galinstan bridges is used to shift down each slot antenna's frequency of operation. Using five microchannels results in a 5-bit reconfigurable antenna. Any combination of frequencies within the ranges of 1.8-3.1 GHz (first band) and 3.2-5.4 GHz (second band) can be obtained. This translates into a discrete tuning ratio of for both bands and overall ratio of 3: 1 (1.8-5.4 GHz) for the antenna. The antenna circuit board is realized using common printed circuit board (PCB) techniques, and the microchannels' polydimethylsiloxane (PDMS) substrate is fabricated using 3-D printing technologies. The circuit board and the PDMS substrates are bonded to each other using a thin layer of spin-coated PDMS. The performance of the antenna is measured, and close agreement between the simulation and measurement is observed.

    DOI

  • Analysis of individual signaling complexes by mMAPS, a flow-proteometric system

    Chou, C.-K., Tsou, P.-H., Hsu, J.L., Lee, H.-H., Wang, Y.-N., Kameoka, J., Hung, M.-C.

    Current Protocols in Molecular Biology   2016   20.11.1-20.11.22  2016年  [国際誌]

     概要を見る

    Signal transduction is essential for maintaining normal cell physiological functions, and deregulation of signaling can lead to diseases such as diabetes and cancers. Some of the major players in signal delivery are molecular complexes composed of proteins and nucleic acids. This unit describes a technique called microchannel for multiparameter analysis of proteins in a single complex (mMAPS) for analyzing and quantifying individual target signaling complexes. mMAPS is a flow-proteometric system that allows detection of individual proteins or complexes flowing through a microfluidic channel. Specific target proteins and nucleic acids labeled by fluorescent tags are harvested from tissues or cultured cells for analysis by the mMAPS system. Overall, mMAPS enables both detection of multiple components within a single complex and direct quantification of different populations of molecular complexes in one setting in a short timeframe and requiring very low sample input.

    DOI PubMed

  • Use of a micro- to nanochannel for the characterization of surface-enhanced Raman spectroscopy signals from unique unctionalized nanoparticles

    Walton, B.M., Huang, P.-J., Kameoka, J., Cote, G.L.

    Journal of Biomedical Optics   21 ( 8 )  2016年

     概要を見る

    A micro- to nanochannel nanoparticle aggregating device that does not require any input energy to organize the particles to a specific location, i.e., no pumps, plugs, heat, or magnets, has been designed and used to characterize the surface-enhanced Raman spectroscopy (SERS) signal from four unique functionalized nanoparticles (gold, silver-gold nanocages, silver nanocubes, and silica-gold nanoshells). The SERS signal was assessed in terms of the peak signal strength from the four different Raman reporter functionalized nanoparticles to determine which nanoparticle had better utility in the channel to provide the most robust platform for a future biological analyte detection device. The innovation used to fabricate the micro- to nanochannel device is described; the TEM images of the nanoparticles are shown; the absorption data for the nanoparticles are given; and the spectral data for the Raman reporter, mercaptobenzoic acid (MBA), are depicted. In the micro- to nanochannel described in this work, 5  μl of 22.3  μM MBA functionalized silver nanocubes were determined to have the strongest SERS enhancement.

    DOI PubMed

  • Ferric plasmonic nanoparticles, aptamers, and magnetofluidic chips: Toward the development of diagnostic surface-enhanced Raman spectroscopy assays

    Marks, H., Huang, P.-J., Mabbott, S., Graham, D., Kameoka, J., Coté, G.

    Journal of Biomedical Optics   21 ( 12 ) 127005 - 127005  2016年  [国際誌]

     概要を見る

    Conjugation of aptamers and their corresponding analytes onto plasmonic nanoparticles mediates the formation of nanoparticle assemblies: molecularly bound nanoclusters that cause a measurable change in the colloid’s optical properties. The optimization of a surface-enhanced Raman spectroscopy (SERS) competitive binding assay utilizing plasmonic “target” and magnetic “probe” nanoparticles for the detection of the toxin bisphenol-A (BPA) is presented. These assay nanoclusters were housed inside three types of optofluidic chips patterned with magnetically activated nickel pads, in either a straight or array pattern. Both Fe 2 O 3 and Fe 2 CoO 4 were compared as potential magnetic cores for the silver-coated probe nanoparticles. We found that the Ag @ Fe 2 O 3 particles were, on average, more uniform in size and more stable than Ag @ Fe 2 CoO 4 , whereas the addition of cobalt significantly improved the collection time of particles. Using Raman mapping of the assay housed within the magnetofluidic chips, it was determined that a 1 × 5 array of 50 ?? ? m square nickel pads provided the most uniform SERS enhancement of the assay (coefficient of variation ? 25 % ) within the magnetofluidic chip. Additionally, the packaged assay demonstrated the desired response to BPA, verifying the technology’s potential to translate magnetic nanoparticle assays into a user-free optical analysis.

    DOI PubMed

  • Fabrication of bacteria environment cubes with dry lift-off fabrication process for enhanced nitrification

    Samarasinghe, S.A.P.L., Shao, Y., Huang, P.-J., Pishko, M., Chu, K.-H., Kameoka, J.

    PLoS ONE   11 ( 11 )  2016年

     概要を見る

    We have developed a 3D dry lift-off process to localize multiple types of nitrifying bacteria in polyethylene glycol diacrylate (PEGDA) cubes for enhanced nitrification, a two-step biological process that converts ammonium to nitrite and then to nitrate. Ammonia-oxidizing bacteria (AOB) is responsible for converting ammonia into nitrite, and nitrite-oxidizing bacteria (NOB) is responsible for converting nitrite to nitrate. Successful nitrification is often challenging to accomplish, in part because AOB and NOB are slow growers and highly susceptible to many organic and inorganic chemicals in wastewater. Most importantly, the transportation of chemicals among scattered bacteria is extremely inefficient and can be problematic. For example, nitrite, produced from ammonia oxidation, is toxic to AOB and can lead to the failure of nitrification. To address these challenges, we closely localize AOB and NOB in PEGDA cubes as microenvironment modules to promote synergetic interactions. The AOB is first localized in the vicinity of the surface of the PEGDA cubes that enable AOB to efficiently uptake ammonia from a liquid medium and convert it into nitrite. The produced nitrite is then efficiently transported to the NOB localized at the center of the PEGDA particle and converted into non-toxic nitrate. Additionally, the nanoscale PEGDA fibrous structures offer a protective environment for these strains, defending them from sudden toxic chemical shocks and immobilize in cubes. This engineered microenvironment cube significantly enhances nitrification and improves the overall ammonia removal rate per single AOB cell. This approach-encapsulation of multiple strains at close range in cube in order to control their interactions-not only offers a new strategy for enhancing nitrification, but also can be adapted to improve the production of fermentation products and biofuel, because microbial processes require synergetic reactions among multiple species.

    DOI PubMed

  • Using flow-proteometric platform to analyze individual signaling complexes in tumor tissue

    Chao-Kai Chou, Heng-Huan Lee, Pei-Hsiang Tsou, Chun-Te Chen, Jung-Mao Hsu, Hirohito Yamaguchi, Ying-Nai Wang, Jennifer L. Hsu, Jin-Fong Lee, Jun Kameoka, Mien-Chie Hung

    CANCER RESEARCH   75  2015年08月

    DOI

  • Hydrogel Micro-system with Multiple Bacteria to Enhance Ammonia Removal Process

    Kameoka,,J, Chu, KH, Samarashinge, SAPL

    In International Technical Conference and Exhibition on Packaging and Integration f Electronic and Photonic Microsystem;ASME     115  2015年  [査読有り]

  • Smart Soft Actuation System

    Kordmahale, S, Kameoka, J

    Ann Materials Sci Eng   2 ( 1 ) 1021  2015年

  • A Reconfigurable Quarter-Mode Substrate Integrated Waveguide Cavity Filter Employing Liquid-Metal Capacitive Loading

    Alireza Pourghorban Saghati, Sina Baghbani Kordmahale, Jun Kameoka, Kamran Entesari

    2015 IEEE MTT-S INTERNATIONAL MICROWAVE SYMPOSIUM (IMS)    2015年

     概要を見る

    A microfiuidically-tunable substrate integrated cavity filter is presented for the first time. Quarter mode cylindrical substrate integrated waveguide (SIW) cavities are used to design an ultra-compact two-pole filter with a center frequency of similar to 1.12 GHz. A corner via is connected to a surface ring gap in order to capacitively load each SIW cavity resonator. Frequency tuning of the filter is achieved using the capacitive loading effect of a liquid metal channel placed on top of the surface gap capacitors. The Polydimethylsiloxane (PDMS) structure including the micro-channel is bonded to the SIW circuit board using a unique fabrication technique. Measured results verify a tuning ratio of 1.72:1 and an insertion loss of 2.5 and 3.45 dB at 1.12 and 0.65 GHz, respectively.

  • Miniature and reconfigurable CPW folded slot antennas employing liquid-metal capacitive loading

    Pourghorban Saghati, A., Singh Batra, J., Kameoka, J., Entesari, K.

    IEEE Transactions on Antennas and Propagation   63 ( 9 ) 3798 - 3807  2015年

     概要を見る

    Microfluidic channels filled with liquid metal are used to realize miniature and reconfigurable CPW folded slot antennas. The method is based on employing the reactive loading effect of fluid metal bridges on top of the CPW slot antenna. As a result of this reactive loading, the frequency of the antenna reduces and the antenna is miniaturized by a factor of 85%. Also, by changing the configuration of filled and empty channels, each channel can be used as a switch. By using two pairs of microfluidic channels, three different frequency bands of 2.4, 3.5, and 5.8 GHz can be achieved. This translates to a switching ratio (f(TR) = f(2)/ f(1)) of more than 2.5. The antenna is realized using common PCB techniques for the antenna circuit board and three-dimensional (3-D) printing technology for PDMS-based microfluidics structure. The antenna circuit board and the PDMS structure are bonded to each other using a very thin spin-coated PDMS layer. Design methodology, simulation, and measurement results of both antenna prototypes are presented. Both the miniature and reconfigurable antennas have similar radiation patterns to a normal CPW folded slot antenna and show low cross-polarization levels at all operating frequencies.

    DOI

  • A Miniaturized Microfluidically Reconfigurable Coplanar Waveguide Bandpass Filter With Maximum Power Handling of 10 Watts

    Pourghorban Saghati, A., Batra, J.S., Kameoka, J., Entesari, K.

    IEEE Transactions on Microwave Theory and Techniques   63 ( 8 ) 2515 - 2525  2015年

     概要を見る

    In this paper, a microfluidically reconfigurable coplanar waveguide (CPW) filter is presented with a tuning range of similar to 1.6:1 and four different states. The passband frequency of the filter is changed based on employing the capacitive loading effect of a liquid metal placed on top of each CPW resonator using three parallel micro-channels. In addition, because of the loading effect of the metal bridges, miniaturization by a factor of 40% is achieved. The filter is digitally tuned from 3.4 to 5.5 GHz with an insertion loss of less than 5.0 dB and a relative bandwidth of 5 +/- 0.35%. The RF power-handling capability of the filter is characterized using a customized measurement setup. It is observed that the filter can be used for input RF powers of up to similar to 20 W for short-duration excitation conditions and 10 W for high-average-power excitation conditions. The filter is realized using common printed circuit board technology and a polydimethylsiloxane structure. Design methodology, simulation, and measurement results of the filter prototype are presented.

    DOI

  • Using micro and nanofluidics with surface enhanced Raman spectroscopy for in vitro blood based biomarker detection

    Gerard L. Cote, Jun Kameoka, Haley Marks

    TRANSLATIONAL BIOPHOTONICS   9155  2014年

     概要を見る

    In this presentation we will discuss the development of a point-of-care optofluidic device that uses gold nanoparticle-based surface enhanced Raman spectroscopy (SERS) for detection of blood biomarkers. SERS approaches have been successfully used for detection of analytes due to the large enhancements provided by the interaction between the light, gold particles, and analyte. However, SERS approaches developed for use to accurately quantify an analyte have suffered from a lack of repeatability. We will describe our SERS optofluidic device with functionalized nanoparticles that helps to overcome these problems and will show results with a focus on blood cardiac biomarkers.

    DOI

  • Microfluidically-Tuned Miniaturized Planar Microwave Resonators

    Alireza Pourghorban Saghati, Jaskirat Batra, Jun Kameoka, Kamran Entesari

    2014 IEEE 15TH ANNUAL WIRELESS AND MICROWAVE TECHNOLOGY CONFERENCE (WAMICON)    2014年

     概要を見る

    This paper presents two frequency tuning techniques, applied to planar microwave lumped LC and CPW resonators. Both methods are based on employing the reactive loading effect of a fluid metal placed on top of planar microwave circuits. The frequency tuning is achieved by moving the fluid metal inside the micro-channel and on top of different sections of each resonator. Realization of tunable resonators using common PCB technology and PDMS structure is demonstrated. The metal fluidic used is a non-toxic eutectic alloy of Gallium, Indium, and Tin known as Galinstan. Two implemented prototypes are measured and tuning ranges of 1.45-2.35 GHz and 2.8-3.9 GHz are achieved for lumped LC and CPW resonators, respectively.

  • A Microfluidically-Switched CPW Folded Slot Antenna

    Alireza Pourghorban Saghati, Jaskirat Batra, Jun Kameoka, Kamran Entesari

    2014 IEEE ANTENNAS AND PROPAGATION SOCIETY INTERNATIONAL SYMPOSIUM (APSURSI)     557 - 558  2014年

     概要を見る

    A microfluidically-switched CPW folded slot antenna is proposed. The switching technique is based on employing the capacitive loading effect of two fluid metal channels placed on top of the CPW slot antenna. By filling the channels with Galinstan, the antenna is reactively loaded and as a result the resonant frequency of the antenna is reduced. Implementation of the microfluidically-switched antenna is realized using the common PCB techniques and PDMS structures. The PDMS structures including the micro-channels are bonded to the circuit board using a very thin layer of spin-coated PDMS employed as the bonding layer. A frequency switching ratio (f(TR)=f(2)/f(1)) of more than an octave (2.6-5.5 GHz) is obtained for the proposed antenna. The antenna shows similar radiation pattern with high purity (Co- to Cross polarization level > 15dB) for both frequency bands.

  • A Microfluidically-Tuned Dual-Band Slot Antenna

    Alireza Pourghorban Saghati, Jaskirat Batra, Jun Kameoka, Kamran Entesari

    2014 IEEE ANTENNAS AND PROPAGATION SOCIETY INTERNATIONAL SYMPOSIUM (APSURSI)     1244 - 1245  2014年

     概要を見る

    A reconfigurable dual-band slot antenna with the capability of independently controlling each band is proposed. The tuning method is based on employing the reactive loading effect of a fluid metal placed on top of each slot antenna. Digital frequency tuning is achieved by using different configurations of four empty and filled channels. This way a 4-bit reconfigurable antenna is achieved in which each resonant frequency of the dual-band response can be controlled separately. Realization of the tunable antenna using common PCB technologies and PDMS structure is demonstrated. The implemented prototype antenna is measured and tuning ranges of 2.2-3.3 GHz and 4.15-5.4 GHz are achieved for the first and second bands, respectively.

  • mMAPS: A flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes

    Chou, C.-K., Lee, H.-H., Tsou, P.-H., Chen, C.-T., Hsu, J.-M., Yamaguchi, H., Wang, Y.-N., Lee, H.-J., Hsu, J.L., Lee, J.-F., Kameoka, J., Hung, M.-C.

    Science Signaling   7 ( 315 ) rs1  2014年  [国際誌]

     概要を見る

    Signal transduction is a dynamic process that regulates cellular functions through multiple types of biomolecular interactions, such as the interactions between proteins and between proteins and nucleic acids. However, the techniques currently available for identifying protein-protein or protein-nucleic acid complexes typically provide information about the overall population of signaling complexes in a sample instead of information about the individual signaling complexes therein. We developed a technique called "microchannel for multiparameter analysis of proteins in a single complex" (mMAPS) that simultaneously detected individual target proteins either singly or in a multicomponent complex in cell or tissue lysates. We detected the target proteins labeled with fluorophores by flow proteometry, which provided quantified data in the form of multidimensional fluorescence plots. Using mMAPS, we quantified individual complexes of epidermal growth factor (EGF) with its receptor EGFR, EGFR with signal transducer and activator of transcription 3 (STAT3), and STAT3 with the acetylase p300 and DNA in lysates from cultured cells with and without treatment with EGF, as well as in lysates from tumor xenograft tissue. Consistent with the ability of this method to reveal the dynamics of signaling protein interactions, we observed that cells treated with EGF induced the interaction of EGF with EGFR and the autophosphorylation of EGFR, but this interaction decreased with longer treatment time. Thus, we expect that this technique may reveal new aspects of molecular interaction dynamics.

    DOI PubMed

  • Flow-proteometric analysis of single signaling complex

    Chao-Kai Chou, Heng-Huan Lee, Pei-Haiang Tsou, Hirohito Yamaguchi, Ying-Nai Wang, Hong-Jen Lee, Jun Kameoka, Mien-Chie Hung

    CANCER RESEARCH   73 ( 8 )  2013年04月

    DOI

  • Diffusion Noise and Photonic Shot Noise at Single Molecule Fluorescence in Micro/Nano-Fluidic Channels

    Kish, L, Kameoka, J, Granqvist, C.-G, Kish, L

    Proc. 2nd Workshop on Nanophotonics and Related Technologies    2012年

  • Optimum drift velocity for single molecule fluorescence bursts in micro/nano-fluidic channels

    Kish, L.L., Kameoka, J., Granqvist, C.G., Kish, L.B.

    Applied Physics Letters   101 ( 4 )  2012年

    DOI

  • Amperometric cholesterol biosensor using layer-by-layer adsorption technique onto electrospun polyaniline nanofibers

    Shin, Y.J., Kameoka, J.

    Journal of Industrial and Engineering Chemistry   18 ( 1 ) 193 - 197  2012年

     概要を見る

    An amperometric cholesterol biosensor was fabricated using electrospun polyaniline nanofibers. Polyaniline was dissolved in chloroform with camphorsulfonic acid, and polystyrene was added in this solution. Using this mixed solution, nanofibers were formed and collected by electrospinning. Then cholesterol oxidase was immobilized onto these fibers using an electrostatic layer-by-layer adsorption technique. Poly(diallyldimethylammonium chloride) was used as the counter ion source. The level of adsorption was examined and evidence of layer-by-layer adsorption was investigated using a quartz crystal microbalance (QCM) technique. A cholesterol biosensor was fabricated from these nanofibers as a working electrode, and it was used to measure the cholesterol concentration. (C) 2011 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

    DOI

  • Microfluidic three-dimensional hydrodynamic flow focusing for the rapid protein concentration analysis

    Hong, S., Tsou, P., Chou, C., Yamaguchi, H., Su, C.B., Hung, M., Kameoka, J.

    Biomicrofluidics   6 ( 2 ) 24132 - 24132  2012年  [国際誌]

     概要を見る

    A simple microfluidic 3D hydrodynamic flow focusing device has been developed and demonstrated quantitative determinations of quantum dot 525 with antibody (QD525-antibody) and hemagglutinin epitope tagged MAX (HA-MAX) protein concentrations. This device had a step depth cross junction structure at a hydrodynamic flow focusing point at which the analyte stream was flowed into a main detection channel and pinched not only horizontally but also vertically by two sheath streams. As a result, a triangular cross-sectional flow profile of the analyte stream was formed and the laser was focused on the top of the triangular shaped analyte stream. Since the detection volume was smaller than the radius of laser spot, a photon burst histogram showed Gaussian distribution, which was necessary for the quantitative analysis of protein concentration. By using this approach, a linear concentration curve of QD525-antibody down to 10 pM was demonstrated. In addition, the concentration of HA-MAX protein in HEK293 cell lysate was determined as 0.283 ± 0.015 nM. This approach requires for only 1 min determining protein concentration. As the best of our knowledge, this is the first time to determinate protein concentration by using single molecule detection techniques.

    DOI PubMed

  • Collagen microsphere production on a chip

    Hong, S., Hsu, H.-J., Kaunas, R., Kameoka, J.

    Lab on a Chip   12 ( 18 ) 3277 - 80  2012年  [国際誌]

     概要を見る

    We have developed an integrated microfluidic material processing chip and demonstrated the rapid production of collagen microspheres encapsulating cells with high uniformity and cell viability. The chip integrated three material processing steps. Monodisperse microdroplets were generated at a microfluidic T junction between aqueous and mineral oil flows. The flow was heated immediately to 37 °C to initiate collagen fiber assembly within a gelation channel. Gelled microspheres were extracted from the mineral oil phase into cell culture media within an extraction chamber. Collagen gelation immediately after microdroplet generation significantly reduced coalescence among microdroplets that led to non-uniform microsphere production. The microfluidic extraction approach led to higher microsphere recovery and cell viability than when a conventional centrifugation extraction approach was employed. These results indicate that chip-based material processing is a promising approach for cell-ECM microenvironment generation for applications such as tissue engineering and stem cell delivery.

    DOI PubMed

  • Log-normal distribution of single molecule fluorescence bursts in micro/nano-fluidic channels

    Kish, L.L., Kameoka, J., Granqvist, C.G., Kish, L.B.

    Applied Physics Letters   99 ( 14 )  2011年

    DOI

  • MEASUREMENT of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS).

    Sungmin Hong, Ying-Nai Wang, Hirohito Yamaguchi, Harinibytaraya Sreenivasappa, Chao-Kai Chou, Pei-Hsiang Tsou, Mien-Chie Hung, Jun Kameoka

    Journal of biomaterials and nanobiotechnology   1 ( 1 ) 31 - 36  2010年10月  [国際誌]

     概要を見る

    We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluorescent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening.

    PubMed

  • Identifying Protein-Protein Interactions in Single Protein Complex level by Microchannel Device

    Chao-Kai Chou, Nan Jing, Hirohito Yamaguchi, Pei-Hsiang Tsou, Jun Kameoka, Mien-Chie Hung

    CANCER RESEARCH   70  2010年04月

    DOI

  • Raster Image Correlation Spectroscopy for Anti-Cancer Drug Screening Based on the Identification of Molecular Dynamics

    Hong, S, Sreenivasappa, H, Yamaguchi, H, Wang, Y.-N, Chou, C, Hung, M.-C, Kameoka, J

    Biophysical Journal   98 ( 3 ) 405a  2010年

  • Functionalized nanoparticles for measurement of biomarkers using a SERS nanochannel platform

    Melodie Benford, Miao Wang, Jun Kameoka, Theresa Good, Gerard Cote

    PLASMONICS IN BIOLOGY AND MEDICINE VII   7577  2010年

     概要を見る

    The overall goal of this research is to develop a new point-of-care system for early detection and characterization of cardiac markers to aid in diagnosis of acute coronary syndrome. The envisioned final technology platform incorporates functionalized gold colloidal nanoparticles trapped at the entrance to a nanofluidic device providing a robust means for analyte detection at trace levels using surface enhanced Raman spectroscopy (SERS). To discriminate a specific biomarker, we designed an assay format analogous to a competitive ELISA. Notably, the biomarker would be captured by an antibody and in turn displace a peptide fragment, containing the binding epitope of the antibody labeled with a Raman reporter molecule that would not interfere with blood serum proteins. To demonstrate the feasibility of this approach, we used C-reactive protein (CRP) as a surrogate biomarker. We functionalized agarose beads with anti-CRP that were placed outside the nanochannel, then added either Rhodamine-6-G (R6G) labeled-CRP and gold (as a surrogate of a sample without analyte present), or R6G labeled CRP, gold, and unlabeled CRP (as a surrogate of a sample with analyte present). Analyzing the spectra we see an increase in peak intensity in the presence of analyte at characteristic peaks for R6G specifically, 1284 and 1567 cm-1. Further, our results illustrate the reproducibility of the Raman spectra collected for R6G-labeled CRP in the nanochannel. Overall, we believe that this method will provide the advantage of sensitivity and narrow line widths characteristic of SERS as well as the specificity toward the biomarker of interest.

    DOI

  • Rapid prototyping of nanofluidic systems using size-reduced electrospun nanofibers for biomolecular analysis

    Park, S.-M., Huh, Y.S., Szeto, K., Joe, D.J., Kameoka, J., Coates, G.W., Edel, J.B., Erickson, D., Craighead, H.G.

    Small   6 ( 21 ) 2420 - 6  2010年  [国際誌]

     概要を見る

    Biomolecular transport in nanofluidic confinement offers various means to investigate the behavior of biomolecules in their native aqueous environments, and to develop tools for diverse single-molecule manipulations. Recently, a number of simple nanofluidic fabrication techniques has been demonstrated that utilize electrospun nanofibers as a backbone structure. These techniques are limited by the arbitrary dimension of the resulting nanochannels due to the random nature of electrospinning. Here, a new method for fabricating nanofluidic systems from size-reduced electrospun nanofibers is reported and demonstrated. As it is demonstrated, this method uses the scanned electrospinning technique for generation of oriented sacrificial nanofibers and exposes these nanofibers to harsh, but isotropic etching/heating environments to reduce their cross-sectional dimension. The creation of various nanofluidic systems as small as 20 nm is demonstrated, and practical examples of single biomolecular handling, such as DNA elongation in nanochannels and fluorescence correlation spectroscopic analysis of biomolecules passing through nanochannels, are provided.

    DOI PubMed

  • Rapid antibiotic efficacy screening with aluminum oxide nanoporous membrane filter-chip and optical detection system

    Tsou, P.-H., Sreenivasappa, H., Hong, S., Yasuike, M., Miyamoto, H., Nakano, K., Misawa, T., Kameoka, J.

    Biosensors and Bioelectronics   26 ( 1 ) 289 - 294  2010年

     概要を見る

    We have developed a filter-chip and optical detection system for rapid antibiotic efficacy screening The filter-chip consisted of a 1-mL reservoir and an anodic aluminum oxide (MO) nanoporous membrane Sample solution with liquid growth media, bacteria, and antibiotics was incubated in the reservoir for a specific period of time The number of live bacteria on the surface of membrane was counted after the incubation with antibiotics and filtration. Using this biosensing system, we have demonstrated a 1-h antibiotic screening for patients' clinical samples, significantly faster than the conventional antibiotic susceptibility tests that typically take more than 24 h. This rapid screening nature makes the filter-chip and detection system ideal for tailoring antibiotic treatment to individual patients by reducing the microbial antibiotic resistance, and improving the survival rate for patients suffering from postoperative infections Published by Elsevier By.

    DOI

  • High speed digital protein interaction analysis using microfluidic single molecule detection system

    Chou, C.-K., Jing, N., Yamaguchi, H., Tsou, P.-H., Lee, H.-H., Chen, C.-T., Wang, Y.-N., Hong, S., Su, C., Kameoka, J., Hung, M.-C.

    Lab on a Chip   10 ( 14 )  2010年

     概要を見る

    The understanding of protein interaction dynamics is important for signal transduction research but current available techniques prove difficult in addressing this issue. Thus, using the microfluidic approach, we developed a digital protein analytical platform and methodology named MAPS (Microfluidic system Analyzing Protein in Single complex) that can measure the amount of target proteins and protein complexes at the digitally single molecule resolution. By counting protein events individually, this system can provide rough protein interaction ratios which will be critical for understanding signal transduction dynamics. In addition, this system only requires less than an hour to characterize the target protein sample, which is much quicker than conventional approaches. As a proof of concept, we have determined the interaction ratios of oncogenic signaling protein complexes EGFR/Src and EGFR/STAT3 before and after EGF ligand stimulation. To the best of our knowledge, this is the first time that the interaction ratio between EGFR and its downstream proteins has been characterized. The information from MAPS will be critical for the study of protein signal transduction quantitation and dynamics.

    DOI PubMed

  • Rapid detection of two-protein interaction with a single fluorophore by using a microfluidic device

    Chou, C.-K., Jing, N., Yamaguchi, H., Tsou, P.-H., Lee, H.-H., Chen, C.-T., Wang, Y.-N., Hong, S., Su, C., Kameoka, J., Hung, M.-C.

    Analyst   135 ( 11 )  2010年

     概要を見る

    We have developed a microfluidics based platform and methodology named MAPS (microfluidic system for analyzing proteins in single complex) for detecting two protein interactions rapidly using a single fluorophore. Target proteins were labelled with Quantum dot 525 (QD525) via specific polyclonal antibodies, and were transported through the microfluidic channel subsequently, where the 375 nm excitation laser light was focused to form a detection volume. Photon bursts from target proteins passing through the detection volume were recorded and their photon burst histograms were plotted which demonstrated roughly the specific protein interaction ratio based on their population and statistical behavior. As a proof of concept, Src/STAT3 protein complex interaction ratios with and without EGF stimulation were obtained by MAPS within 1 h and the results were well matched with the one obtained by the conventional immunoprecipitation/Western blot (IP/WB).

    DOI PubMed

  • Detection of Cardiac Biomarkers Exploiting Surface Enhanced Raman Scattering (SERS) using a Nanofluidic Channel Based Biosensor towards Coronary Point-of-Care Diagnostics

    Melodie E. Benford, Miao Wang, Jun Kameoka, Gerard L. Cote

    PLASMONICS IN BIOLOGY AND MEDICINE VI   7192  2009年

     概要を見る

    According to the World Health Organization, cardiovascular disease is the most common cause of death in the world. In the US, over 115 million people visit the emergency department (ED), 5 million of which may have acute coronary syndrome (ACS). Cardiac biomarkers can provide early identification and diagnosis of ACS, and can provide information on the prognosis of the patient by assessing the risk of death. In addition, the biomarkers can serve as criteria for admission, indicate possibility of re-infarction, or eliminate ACS as a diagnosis altogether. We propose a SERS-based multi-marker approach towards a point-of-care diagnostic system for ACS. Using a nanofluidic device consisting of a microchannel leading into a nanochannel, we formed SERS active sites by mechanically aggregating gold particles (60 nm) at the entrance to the nanochannel (40 nmx1 mu m). The induced capillary flow produces a high density of aggregated nanoparticles at this precise region, creating areas with enhanced electromagnetic fields within the aggregates, shifting the plasmon resonance to the near infrared region, in resonance with incident laser wavelength. With this robust sensing platform, we were able to obtain qualitative information of brain natriuretic peptide (biomarker of ventricular dysfunction or pulmonary stress), troponin I (biomarker of myocardial necrosis), and C-reactive protein (biomarker of inflammation potentially caused by atherosclerosis).

    DOI

  • Optofluidic device for ultra-sensitive detection of proteins using surface-enhanced Raman spectroscopy

    Wang, M., Benford, M., Jing, N., Coté, G., Kameoka, J.

    Microfluidics and Nanofluidics   6 ( 3 ) 411 - 417  2009年

     概要を見る

    An optofluidic device is reported in this paper that can highly improve the robustness of surface-enhanced Raman scattering (SERS) detection and provide fingerprint information of proteins with a concentration in the nanogram per liter range within minutes. Moreover, the conformational change of protein can also be obtained using this device. Fabricated by standard photolithography processes, the optofluidic device has a step microfluidic-nanofluidic structure, which provides robust SERS detection. The sensitivity of the device is investigated using insulin and albumin as target analytes at a concentration of 0.9 ng/L. The ability to detect conformational changes of proteins using this technology is also shown by probing these analytes before and after their denaturation.

    DOI

  • Electrospun nanofiber biosensor for measuring glucose concentration

    Shin, Y.J., Wang, M., Kameoka, J.

    Journal of Photopolymer Science and Technology   22 ( 2 ) 235 - 237  2009年

     概要を見る

    An amperometric biosensor was fabricated using electospun composite nanofiber membra ne which are consisted of among polyaniline-polystylene nanofibers. Polyaniline was reacted with camphorsulfonic acid to produce a salt, which was then dissolved in chloroform containing polystyrene. By electrospinning this polymer solution, nanofibers were formed and collected at the counter electrodes. Glucose oxidase was immobilized onto these nanofiber matt using an electrostatic layer-by-layer adsorption technique. In this method, poly(diallyldimethylammonium chloride) was used as the counter ion source. The electrical property of this composite nanofiber was investigated from these nanofibers as a working electrode, and used to measure the glucose concentration accurately.

    DOI

  • Measurements of refractive index change due to positive ions using a surface plasmon resonance sensor

    Ko, H., Kameoka, J., Su, C.B.

    Sensors and Actuators, B: Chemical   143 ( 1 )  2009年

    DOI

  • Single molecule Identification via Nanofluidic Immunospectroscopy

    Kameoka J, Jing N

    Biolphysical Journal   94   2486  2008年

  • Determination of Protein Concentration with Two-Dimensional (2D) Photon Burst Diagrams Using Microfluidic Channel

    Jing, N, Su, C. B, Hung, C.-K. C. M.-C, Kameoka J

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS    2008年

  • An optofluidic device for surface enhanced Raman spectroscopy (vol 7, pg 630, 2007)

    Miao Wang, Nan Jing, I-Hsien Chou, Gerard L. Cote, Jun Kameoka

    LAB ON A CHIP   8 ( 2 ) 362 - 362  2008年

  • In vitro detection of beta amyloid exploiting surface enhanced Raman scattering (SERS) using a nanofluidic biosensor

    Melodie E. Benford, I-Hsien Chou, Hope T. Beier, Miao Wang, Jun Kameoka, Theresa A. Good, Gerard L. Cote

    PLASMONICS IN BIOLOGY AND MEDICINE V   6869  2008年

     概要を見る

    Alzheimer's disease (AD), a neurodegenerative disease and the most common cause of dementia, affects 4.5 million people according to the 2000 US census and is expected to triple to 13.2 million by the year 2050. Since no definitive pre-mortem. tests exist to distinguish AD from mild cognitive impairment due to the natural aging process, we focus on detecting the beta amyloid (A beta) protein, the primary component of the senile plaques characteristic of AD. We specifically detect cytotoxic species of A beta by exploiting surface enhanced Raman scattering (SERS). Using a nanofluidic device with a bottleneck shape (a microchannel leading into a nanochannel); we trapped gold colloid particles (60 nm) at the entrance to the nanochannel, with A beta restricted within the interstices between the aggregated nanoparticles. The continuous flow generated from pumping the solution into the device produced size-dependent trapping of the gold colloid particles, resulting in a high density of aggregated nanoparticles at this precise region, creating localized "hot spots" in the interstitial region between nanoparticles, and shifting the plasmon resonance to the near infrared region, in resonance with incident laser wavelength. With this robust sensing platform, we were able to obtain concentration-dependent SERS spectra of A beta and of different proteins that would be present in the cerebrospinal fluid of healthy people and people with Alzheimer's disease.

    DOI

  • Optofluidic device for molecular detection via surface enhanced Raman spectroscopy

    Miao Wang, Nan Jing, I-Hsien Chou, Gerard L. Cote, Jun Kameoka

    2008 DIGEST OF THE LEOS SUMMER TOPICAL MEETINGS     191 - +  2008年

  • Protein functionalized micro hydrogel features for cell-surface interaction

    Bhatnagar, P., Nixon, A.J., Kim, I., Kameoka, J.

    Biomedical Microdevices   10 ( 4 ) 567 - 571  2008年

     概要を見る

    Cross-linked hydrogel features have been patterned using subtractive lift-off of polymerized hydrogel film. Projection lithography and oxygen plasma etch was used to pattern parylene C polymer film. Molecular self-assembly of polymerizable monolayer was obtained in solution-phase and acrylamide based hydrogel was polymerized using free-radical polymerization on this substrate. Parylene C film was mechanically lifted-off to remove the blanket hydrogel film and micro hydrogel features (mu hf) were obtained attached to the predefined patterns in the range from 1 to 60 mu m. The mu hf were functionalized with aldehyde functional groups, and proteins were coupled to them using Schiff base chemistry followed by reductive amination. Interaction of mesenchymal stem cells with transforming growth factor-beta 1 (TGF-beta 1) functionalized mu hf was studied, and TGF-beta 1 was found to retain its tumor suppression activity.

    DOI

  • The fabrication and testing of electrospun silica nanofiber membranes for the detection of proteins

    Tsou, P.-H., Chou, C.-K., Saldana, S.M., Hung, M.-C., Kameoka, J.

    Nanotechnology   19 ( 44 )  2008年

    DOI

  • Nanofluidic devices for single molecule identification

    Jing, N., Kameoka, J., Su, C.B., Chou, C.-K., Hung, M.-C.

    Journal of Photopolymer Science and Technology   21 ( 4 )  2008年

    DOI

  • Forty-four pass fibre-optic loop for improving the sensitivity of surface plasmon resonance sensors

    Su, C.B., Kameoka, J.

    Measurement Science and Technology   19 ( 1 )  2008年

     概要を見る

    A 44-pass fibre-optic surface plasmon resonance sensor that enhances detection sensitivity according to the number of passes is demonstrated for the first time. The technique employs a fibre-optic recirculation loop that passes the detection spot 44 times, thus enhancing sensitivity by a factor of 44. Presently, the total number of passes is limited by the onset of lasing action of the recirculation loop. This technique offers a significant sensitivity improvement for various types of plasmon resonance sensors that may be used in chemical and biomolecule detections.

    DOI

  • Monodispersed polygonal water droplets in microchannel

    Mehrotra, R., Jing, N., Kameoka, J.

    Applied Physics Letters   92 ( 21 )  2008年

    DOI

  • Nanofluidic biosensing for β-amyloid detection using surface enhanced raman spectroscopy

    Chou, I.-H., Benford, M., Beier, H.T., Coté, G.L., Wang, M., Jing, N., Kameoka, J., Good, T.A.

    Nano Letters   8 ( 6 )  2008年

     概要を見る

    Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounder proteins commonly found in cerebral spinal fluid.

    DOI PubMed

  • Nanofluidic channel based biosensor using Surface Enhanced Raman spectroscopy (SERS)

    I-Hsien Chou, Hope T. Beier, Maio Wang, Nan Jing, Jun Kameoka, Gerard L. Cote

    ULTRASENSITIVE AND SINGLE-MOLECULE DETECTION TECHNOLOGIES II   6444  2007年

     概要を見る

    The Raman scattering signature of molecules has been demonstrated to be greatly enhanced, on the order of 10(6)-10(12) times, on roughened metal surfaces and clustered structures such as aggregated colloidal gold. Here we describe a method that improves reproducibility and sensitivity of the substrate for surface enhanced Raman spectroscopy (SERS) by using a nanofluidic trapping device. This nanofluidic device has a bottle neck shape composed of a microchannel leading into a nano channel that causes size-dependent trapping of nanoparticles. The analyte and Au nanoparticles, 60 nm in diameter, in aqueous solution was pumped into the channel. The nanoparticles which were larger than the narrow channel are trapped at the edge of the channel to render an enhancement of the Raman signal. We have demonstrated that the Raman scattering signal enhancement on a nanochannel-based colloidal gold cluster is able to detect 10 pM of adenine, the test analyte, without chemical modification. The efficiency and robustness of the device suggests potential for single molecule detection and multicomponent detection for biological applications and/or biotoxins.

    DOI

  • Sorting of silica nanocups by diameter during fabrication process

    Deotare, P., Kameoka, J.

    Journal of Nanomaterials   2007  2007年

     概要を見る

    We demonstrated a new technique to sort nanoparticles based on their dimensions. Due to the interactions between charged droplets and a nonlinear electrostatic field, nanoparticles with different dimensions were deposited at different spatial locations on a given target substrate. By using this principle, we have been able to sort nanocups into three groups with mean diameters of 0.31 mu m, 0.7 mu m, and 1.1 mu m and a standard deviation of 20%. This technique improves the nanoparticle fabrication process not only by decreasing the standard deviation of its dimensions but also by increasing its yield since nanoparticles with different mean diameters can be generated at the same time. Copyright (c) 2007.

    DOI

  • Characterization of a field-assist multi-pass fibre optic surface plasmon resonance sensor

    Ko, H., Su, C.B., Kameoka, J.

    Measurement Science and Technology   18 ( 9 )  2007年

    DOI

  • An optofluidic device for surface enhanced Raman spectroscopy

    Wang, M., Jing, N., Chou, I.-H., Cote, G.L., Kameoka, J.

    Lab on a Chip   7 ( 5 ) 630 - 632  2007年

     概要を見る

    We have developed an optofluidic device that improves the sensitivity of surface enhanced Raman spectroscopy (SERS) when compared to other SERS approaches. This device has a pinched and step microchannel-nanochannel junction that can trap and assemble nanoparticles/target molecules into optically enhanced SERS active clusters by using capillary force. These SERS active clusters provide an electromagnetic enhancement factor of similar to 10(8). In addition, due to the continuous capillary flow that can transport nanoparticles/target molecules into the junction sites, the numbers of nanoparticles/target molecules and SERS active sites are increased. As a result, the detection limit of SERS for adenine molecules was better than 10 pM.

    DOI

  • Fabrication of nanofluidic filter device for Surface Enhanced Raman Spectroscopy

    Wang, M, Jing, N, Baldwin, A, Coté, G. L, Kameoka J

    International Microprocesses and nanotechnology Conference     44 - 45  2006年

  • Photo-crosslinked Porous PEG Hydrogel Membrane via Electrospinning

    Ko Hyungduk, Kameoka Jun

    Journal of photopolymer science and technology   19 ( 3 ) 413 - 418  2006年

    DOI CiNii

  • Fabrication of silica nanocomposite-cups using electrospraying

    Deotare, P.B., Kameoka, J.

    Nanotechnology   17 ( 5 )  2006年

    DOI

  • Electrospinning of silica nanochannels for single molecule detection

    Wang, M., Jing, N., Su, C.B., Kameoka, J., Chou, C.-K., Hung, M.-C., Chang, K.-A.

    Applied Physics Letters   88 ( 3 )  2006年

    DOI

  • Properties of an optical multipass surface plasmon resonance technique

    Su, C.B., Kameoka, J., Ilic, B., Chu, K.-H., Chang, K.-A.

    Applied Physics Letters   89 ( 7 )  2006年

     概要を見る

    A fiber optic four-pass surface plasmon resonance technique with an approach to increase the number of passes to any arbitrary number is described. Within multipass regime, reflections off the gold sample surface that reduce the reflectivity to less than 0.1% are achieved by using a fiber optic collimator, a reflector, and a corner cube prism. In this case, the optical beam emits from and returns to the collimator. This technique holds the potential for significantly increasing the detection sensitivity of surface plasmon resonance device.

    DOI

  • Fabrication of Silica Nanochannels via Scanned Coaxial Electrospinning

    Wang, M, Jing, N, Chou, C. K, Hung, M, Kameoka J

    9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005     244 - 246  2005年

  • Polymeric Nanowire Architectures and Nanodevices

    Liu, H, Kameoka, J, Verbridge, S, Craighead, H. G

    NSTI Nanotechnology Conference and Trade Show-NSTI Nanotech     209 - 212  2005年

  • A polymeric microchip with integrated tips and in situ polymerized monolith for electrospray mass spectrometry

    Yang, Y., Li, C., Kameoka, J., Lee, K.H., Craighead, H.G.

    Lab on a Chip   5 ( 8 )  2005年

    DOI

  • Fabrication of ultrathin ZrO<inf>2</inf> nanofibers by electrospinning

    Jing, N., Wang, M., Kameoka, J.

    Journal of Photopolymer Science and Technology   18 ( 4 )  2005年

    DOI

  • Measurement of the Young's moduli of individual polyethylene oxide and glass nanofibres

    Bellan, L.M., Kameoka, J., Craighead, H.G.

    Nanotechnology   16 ( 8 )  2005年

    DOI

  • Fabrication of Nanomechanical Oscillators Using Electrospun Polymeric Fibers

    Verbridge, S, Czaplewski, D, Kameoka, J, Craighead, H

    APS March Meeting Abstracts     J14-002  2004年

  • Fabrication of blended polyphenylenevinylene nanowires by scanned electrospinning

    Kameoka, J., Ilic, R., Czaplewski, D., Mathers, R.T., Coates, G.W., Craighead, G.

    Journal of Photopolymer Science and Technology   17 ( 3 )  2004年

    DOI

  • Polymeric nanowire chemical sensor

    Liu, H., Kameoka, J., Czaplewski, D.A., Craighead, H.G.

    Nano Letters   4 ( 4 )  2004年

    DOI

  • Polymeric nanowire architecture

    Kameoka, J., Czaplewski, D., Liu, H., Craighead, H.G.

    Journal of Materials Chemistry   14 ( 10 )  2004年

    DOI

  • Fabrication of suspended silica glass nanofibers from polymeric materials using a scanned electrospinning source

    Kameoka, J., Verbridge, S.S., Liu, H., Czaplewski, D.A., Craighead, H.G.

    Nano Letters   4 ( 11 )  2004年

    DOI

  • Nanomechanical oscillators fabricated using polymeric nanofiber templates

    Czaplewski, D.A., Verbridge, S.S., Kameoka, J., Craighead, H.G.

    Nano Letters   4 ( 3 )  2004年

    DOI

  • Novel Polymeric Nanofiber Deposition Processing

    Kameoka, J

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY; AMER CHEMICAL SOC 1155 16TH ST, NW, WASHINGTON, DC 20036 USA   226   U443  2003年

  • Novel Polymeric Nanofiber Deposition Processing for MEMS Device Fabrication

    Czaplewski, D, Kameoka, J, Mathers, R, Coates, G, Craighead, H

    Polymer Preprints   44 ( 2 ) 117  2003年  [査読有り]

  • An arrow shaped silicon tip for polymeric nanofiber fabrication

    Kameoka, J., Czaplewski, D., Craighead, H.G.

    Journal of Photopolymer Science and Technology   16 ( 3 )  2003年

    DOI

  • Fabrication of oriented polymeric nanofibers on planar surfaces by electrospinning

    Kameoka, J., Craighead, H.G.

    Applied Physics Letters   83 ( 2 )  2003年

    DOI

  • Creating Biological Membranes on the Micron Scale: Forming Patterned Lipid Bilayers Using a Polymer Lift-Off Technique

    Orth, R.N., Kameoka, J., Zipfel, W.R., Ilic, B., Webb, W.W., Clark, T.G., Craighead, H.G.

    Biophysical Journal   85 ( 5 )  2003年

    DOI

  • Nonlithographic approach to nanostructure fabrication using a scanned electrospinning source

    Czaplewski, D., Kameoka, J., Craighead, H.G.

    Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures   21 ( 6 )  2003年

    DOI

  • Chip-Based P450 Drug Metabolism Coupled to Electrospray Ionization-Mass Spectrometry Detection

    Benetton, S., Kameoka, J., Tan, A., Wachs, T., Craighead, H., Henion, J.D.

    Analytical Chemistry   75 ( 23 )  2003年

    DOI

  • Nanofluidic channels with elliptical cross sections formed using a nonlithographic process

    Czaplewski, D.A., Kameoka, J., Mathers, R., Coates, G.W., Craighead, H.G.

    Applied Physics Letters   83 ( 23 )  2003年

    DOI

  • A scanning tip electrospinning source for deposition of oriented nanofibres

    Kameoka, J., Orth, R., Yang, Y., Czaplewski, D., Mathers, R., Coates, G.W., Craighead, H.G.

    Nanotechnology   14 ( 10 )  2003年

    DOI

  • Polymeric Trapezoidal Microelectrospray Emitter Integrated with a Microfluidic Chip

    Kameoka, J, Orth, R, Ilic, B, Czaplewski, D, Craighead, H. G

    2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology     62 - 65  2002年

  • Micromachined Polymeric Tip as An Electrospray Ionization Source

    Jun Kameoka,Reid Orth, Harld. G. Craighead

    Micro Total Analysis Systems 2002     500 - 502  2002年

  • An electrospray ionization source for integration with microfluidics

    Kameoka, J., Orth, R., Ilic, B., Czaplewski, D., Wachs, T., Craighead, H.G.

    Analytical Chemistry   74 ( 22 )  2002年

    DOI

  • Polymeric Microfluidic Device for Separation of Small Molecules

    Kameoka, J, Zhong, H, Henion, J, Craighead, H. G

    . In Microfluidics and BioMEMS; International Society for Optics and Photonics   4560   227 - 235  2001年

  • A polymeric capillary electrophoretic microfluidic device for separation and electrospray ionization of small molecules

    J. Kameoka, Hongwei Zhong, J. Henion, D.B. Mawhinney, H.G. Craighead

    Digest of Papers. Microprocesses and Nanotechnology 2001. 2001 International Microprocesses and Nanotechnology Conference (IEEE Cat. No.01EX468)    2001年

    DOI

  • Surface plasmon resonance sensors utilizing microfabricated channels

    Furuki, M., Kameoka, J., Craighead, H.G., Isaacson, M.S.

    Sensors and Actuators, B: Chemical   79 ( 1 )  2001年

    DOI

  • A polymeric microfluidic chip for CE/MS determination of small molecules

    Kameoka, J., Craighead, H.G., Zhang, H., Henion, J.

    Analytical Chemistry   73 ( 9 )  2001年

    DOI

  • Nanofabricated refractive index sensor based on photon tunneling in nanofluidic channel

    Kameoka, J., Craighead, H.G.

    Sensors and Actuators, B: Chemical   77 ( 3 )  2001年

    DOI

▼全件表示

書籍等出版物

  • 2nd Edition Nanofluidics

    Wang, M, Huang, PJ, Chou, Ck, Tsou, PH, Yamaguchi, H, Hung, MC, Kameoka, J( 担当: 共編者(共編著者),  担当範囲: Nanofluidic strategies for Cancer Research)

    Royal society of chemistry publishing  2016年

  • Encyclopedia of Nanoscience and Nanotechnology

    ( 担当: 共編者(共編著者))

    2011年08月

  • Handbook of Optofluidics

    ( 担当: 共編者(共編著者))

    CRC Press  2010年03月

  • 1st Edition Nanofluidics

    ( 担当: 分担執筆,  担当範囲: Nanostructures for single molecule detection)

    2007年07月

Misc

  • マイクロチューブを用いたアダプティブ超音波集束システムのフォノニック・メタ構造のトポロジー最適化

    安藤真, 高橋徹, 植田毅, 亀岡遵, 飯盛浩司, 松本敏郎

    日本機械学会計算力学講演会論文集(CD-ROM)   30th  2017年

    J-GLOBAL

  • Microfluidic 3D Hydrodynamic Flow Focusing for the rapid protein analysis

    Jun Kameoka, Sungmin, Hong

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2011-2012     16 - 17  2012年

  • Development of microchannel protein complex detection system

    Jun Kameoka, Mien Chie Hung, Chao kai Chou, Nick Jing, Hirohito Yamaguchi

    Department of Defense Health Program Brest Cancer Research Program Conference "Era of hope"     P35-31  2011年08月

  • Dissecting protein complex interactions in breast cancer by photon burst spectroscopy

    Heng-Huan Lee, Chao Kai Chou, Hirohito Yamaguchi, Jun Kameoka, Mien Chie Hung

    Department of Defense Health Program Brest Cancer Research Program Conference "Era of hope"     P35-29  2011年08月

  • Single Protein Detection Using a microfludiic system

    Jun Kameoka, Sungmin Hong

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2010-2011     32 - 33  2011年

  • Microfluidic Channel Fabrication for single molecule detection

    Jun Kameoka, Sungmin Hong

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2009-2010     32 - 33  2010年

  • Nanofluidic Biosensor for SERS

    Jun Kameoka, Miao Wang

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2008-2009     54 - 55  2010年

  • Hydrodynamic Focusing Device for single molecule detection

    Jun Kameoka, Sungmin Hong

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2008-2009     52 - 53  2009年

  • Fabrication of Nanofluidic Filter Device for Surface Enhanced Raman Spectroscopy

    Jun Kameoka, Nan Jing

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2006-2007     104 - 105  2007年

    担当区分:筆頭著者

  • Fabrication of fused silica nanofluidic channels

    Jun Kameoka, Nan Jing

    Cornell Nanoscale Science and Technology Facility Research Accomplishments 2005-2006     74 - 75  2006年

  • Nanochannels for the Identification of Single Molecules

    Kameoka, J, Jing, N, Wang, M

    SPIE e-news letter,    2006年

  • Integration of polymeric single nanofiber with microfabricated syracture

    Jun Kameoka, Harold Craighead

    Second International conference on Molecular electronics and bioelectronics     55  2003年03月

▼全件表示

産業財産権

  • 口金、これを用いたナノ繊維の製造方法およびナノ繊維

    特許第4975327号

    中野 圭洋, 亀岡 遵

    特許権

    J-GLOBAL

  • ナノ繊維

    特許第4778797号

    中野 圭洋, 亀岡 遵

    特許権

    J-GLOBAL

  • 微生物検出方法

    中野 圭洋, 亀岡 遵, 安池 雅之

    特許権

    J-GLOBAL

  • チップ

    中野 圭洋, 亀岡 遵, 安池 雅之

    特許権

    J-GLOBAL

  • 測定装置および測定方法

    中野 圭洋, 亀岡 遵

    特許権

    J-GLOBAL

  • Device for spectroscopic detection and monitoring of biologically relevant molecules

    特許US 9, 989,471, US 10,520,444.

    Po-jung Huang, Gerald Cote, Michell B. Robinson, Haley L. Marks, Jun Kameoka

    特許権

  • Method for detecting molecule-molecule interactions with a single detection channel

    特許US 8,586,316

    Jun Kameoka, Jing, Nan, Hung, Mien-chie, Chu Chao-Kai

    特許権

  • Microfiber supported nanofiber membrane

    特許US 7,591,883

    Jun Kameoka, Keiyo Nakano

    特許権

  • Scanned Source Oriented Nanofiber Formation

    特許US 7,537,807, US 8,413,603, US 8,858,815

    Jun Kameoka, Harold Craighead

    特許権

  • Nanofabricated photon tunneling based sensor

    特許US 7,267,797

    Jun Kameoka, Harold Craighead

    特許権

  • Electrospray emitter for microfluidic channel

    特許US 7105810

    Jun Kameoka, Harold Craighead

    特許権

  • Electrospray emitter for microfluidic channel

    特許US 7081622

    Jun Kameoka, Harold Craighead

    権利者: Cornell University

    特許権

▼全件表示

受賞

  • TEES 研究優秀賞

    2014年12月   テキサス州  

共同研究・競争的資金等の研究課題

  • Precise Advanced Technologies and Health Systems for Underserved Populations

    National Science Fundation 

    研究期間:

    2017年10月
    -
    2027年09月
     

    Gerald Cote

  • Low cost paper sensor for surveillance of cereal crops

    Bill and Melinda Gates Fundation 

    研究期間:

    2018年12月
    -
    2021年06月
     

  • Advanced methods for mimicking the osteogenic niche to heal bone

    National Institute of Health 

    研究期間:

    2014年04月
    -
    2021年06月
     

    Roland Kaunus

  • Microfluidic multi carrier impedance sensor platform with sulfonated-Poly(ethylene glycol) diacrylate absorber for PFOS and PFOA detection

    Texas State 

    研究期間:

    2017年07月
    -
    2018年08月
     

    Bella Chu, Kamran Entesali

  • Enhancing bone regeneration by mimicking the osteogenic niche

    研究期間:

    2014年02月
    -
    2018年04月
     

    Roland Kaunas

  • Portable nanofluidic aptomer-SERS instrument for measurement of chemical exposure

    Ntional Institete of Health  SBIR Phase II

    研究期間:

    2016年02月
    -
    2018年01月
     

    Gerald Cote

  • High throughput flow proteometric system in screening functional complexes as cancer biomarker

    Cancer Prevention and Research Institute of Texas 

    研究期間:

    2014年12月
    -
    2017年11月
     

    Mien Chie Hung

  • Robust surface enhanced Raman biosensor using a novel optofluidic platform technology

    National Sciece Fundation  CBET

    研究期間:

    2011年09月
    -
    2014年08月
     

    Gerald Cote

  • Portable nanofluidic aptamer-SERS instrument for measurement of chemical exposure

    National Institute of Health  SBIR Phase I

    研究期間:

    2012年10月
    -
    2013年08月
     

    Gerald Cote

  • Nanofluidic flow-proteometry for the determination of cancer biomarker concentration

    National Institute of Health 

    研究期間:

    2009年09月
    -
    2011年08月
     

    Mien Chie Hung

  • NIRT-Hierarchical Manufacturing and Modeling Approaches for Phase Transforming Nanostructures

    研究期間:

    2007年07月
    -
    2011年06月
     

    Ibrahim Karaman, Dimitris Lagoudas

  • Development of photon burst spectroscopy

    Department of Defense 

    研究期間:

    2008年09月
    -
    2010年08月
     

    Mien Chie Hung

▼全件表示

講演・口頭発表等

  • International Association of Advanced Materials

    Jun Kameoka  [招待有り]

    International Association of Advanced Materials Fellow lecture  

    発表年月: 2020年12月

  • Automated nanofluidic platfrom for dissecting DNA-protein interaction

    Jun Kameoka

    28th Anniversary World Congress on Biosensors  

    開催年月:
    2018年06月
     
     
  • 医工学連携プロジェクト

    Jun Kameoka  [招待有り]

    医学研究の基礎を語り合う集い  

    発表年月: 2017年10月

    開催年月:
    2017年10月
    -
     
 

現在担当している科目

担当経験のある科目(授業)

  • 電気材料概論

    テキサスA&M 大学  

    2015年01月
    -
    継続中
     

  • ナノバイオテクノロジー

    テキサスA&M 大学  

    2005年01月
    -
    継続中
     

  • ナノテク加工技術

    テキサスA&M 大学  

    2004年09月
    -
    継続中
     

  • マイクロマシン概論

    テキサスA&M 大学  

    2017年01月
    -
    2018年05月
     

  • ソフトロボティクス

    テキサスA&M 大学  

    2015年09月
    -
    2018年05月
     

  • センサーテクノロジー

    テキサスA&M 大学  

    2013年01月
    -
    2013年05月
     

  • ナノフォトニクス

    テキサスA&M 大学  

    2007年01月
    -
    2010年05月
     

  • 応用電磁気学

    テキサス A&M大学  

    2009年01月
    -
    2009年05月
     

  • マイクロ電子工学

    テキサスA&M 大学  

    2008年01月
    -
    2008年05月
     

▼全件表示

 

委員歴

  • 2020年10月
    -
    継続中

    アメリカ国立科学財団  バイオセンシング プログラム査読委員

  • 2018年04月
    -
    継続中

    MDPI バイオセンサー  編集委員

  • 2018年03月
    -
    継続中

    MDPI センサー  編集委員

  • 2017年06月
    -
    2019年05月

    米国国立衛生研究所  NIBIB 査読委員

  • 2013年05月
    -
    2013年06月

    米国国立衛生研究所  NIH-SBIR 査読委員

  • 2007年05月
    -
    2011年04月

    米国国立衛生研究所  IMAT プログラム 査読委員

  • 2009年05月
    -
    2009年07月

    シンガポール学術振興会  電子工学査読委員

  • 2006年05月
    -
    2009年04月

    フォトポリマー学会  国際諮問委員

▼全件表示