Updated on 2023/09/25

写真a

 
YAMADA, Seiya
 
Affiliation
Faculty of Human Sciences, School of Human Sciences
Job title
Assistant Professor(without tenure)

Research Experience

  • 2022.04
    -
    Now

    Waseda University   Faculty of Human Sciences   Assistant Professor

  • 2021.04
    -
    2022.03

    Waseda University   Faculty of Human Sciences   Research Associate

  • 2020.04
    -
    2021.03

    日本学術振興会 特別研究員DC1

  • 2020.04
    -
    2021.03

    Waseda University

Education Background

  • 2020.04
    -
    2022.03

    Waseda University  

  • 2018.04
    -
    2020.03

    Waseda University  

  • 2014.04
    -
    2018.03

    早稲田大学   人間科学部   人間情報科学科  

Professional Memberships

  • 2018.04
    -
    Now

    日本神経科学学会

  • 2017.04
    -
    Now

    日本分子生物学会

Research Areas

  • Molecular biology / Neuroscience-general

Research Interests

  • cortical development, neural stem cells, neurogenesis, purine metabolism, purinosomes, post-translational modifications, SUMOylation, spines, actin, mitochondria, liver, NASH

Awards

  • FENS-IBRO/PERC Travel Grant Award

    2022.07   FENS Forum 2022  

    Winner: Seiya Yamada, Ayaka Sato, Shin-ichi Sakakibara

  • 小野梓記念賞 (学術賞)

    2020.03   早稲田大学  

    Winner: 山田晴也

  • 優秀発表賞

    2019.07   NEURO2019(第42回日本神経科学大会/第62回神経化学会大会)  

    Winner: 山田晴也, 秋山博紀, 榊原伸一

  • ジュニア研究者ポスター賞

    2018.07   第41回日本神経科学大会  

    Winner: 山田晴也, 秋山博紀, 榊原伸一

 

Papers

  • Spatiotemporal regulation of de novo and salvage purine synthesis during brain development

    Tomoya Mizukoshi, Seiya Yamada, Shin-ichi Sakakibara

    eNeuro (Accepted in press)    2023.03  [Refereed]

    Authorship:Corresponding author

     View Summary

    Abstract

    The levels of purines, essential molecules to sustain eukaryotic cell homeostasis, are regulated by the coordination of thede novoand salvage synthesis pathways. In the embryonic central nervous system (CNS), thede novopathway is considered crucial to meet the requirements for the active proliferation of neural stem/progenitor cells (NSPCs). However, how these two pathways are balanced or separately utilized during CNS development remains poorly understood. In this study, we showed a dynamic shift in pathway utilization, with greater reliance on thede novopathway during embryonic stages and on the salvage pathway at postnatal–adult stages. The pharmacological effects of various purine synthesis inhibitorsin vitroand the expression profile of purine synthesis enzymes indicated that NSPCs in the embryonic cerebrum mainly utilize thede novopathway. Simulatenously, NSPCs in the cerebellum require both thede novoand the salvage pathways.In vivoadministration ofde novoinhibitors resulted in severe hypoplasia of the forebrain cortical region, indicating a gradient of purine demand along the anteroposterior axis of the embryonic brain, with cortical areas of the dorsal forebrain having higher purine requirements than ventral or posterior areas such as the striatum and thalamus. This histological defect of the neocortex was accompanied by strong downregulation of the mechanistic target of rapamycin complex 1 (mTORC1)/ribosomal S6 kinase (S6K)/S6 signaling cascade, a crucial pathway for cell metabolism, growth, and survival. These findings indicate the importance of the spatiotemporal regulation of both purine pathways for mTORC1 signaling and proper brain development.

    DOI

  • Nwd1によるプリノソーム形成を介した大脳皮質発生機構

    山田晴也, 佐藤彩佳, 榊原伸一

    Medical Science Digest   49 ( 3 ) 32 - 33  2023.03

    Authorship:Lead author

  • Inka2 expression in smooth muscle cells and its involvement in cell migration

    Seiya Yamada, Akinori Tokunaga, Shin-ichi Sakakibara

    Biochemical and Biophysical Research Communications    2022.12  [Refereed]

    Authorship:Lead author, Corresponding author

    DOI

    Scopus

  • Identification and expression profile of novel STAND gene Nwd2 in the mouse central nervous system

    Seiya Yamada, Ryutaro Furukawa, Shin-ichi Sakakibara

    Gene Expression Patterns   46   119284 - 119284  2022.12  [Refereed]

    Authorship:Lead author, Corresponding author

    DOI

    Scopus

    1
    Citation
    (Scopus)
  • Inka2, a novel Pak4 inhibitor, regulates actin dynamics in neuronal development

    Seiya Yamada, Tomoya Mizukoshi, Akinori Tokunaga, Shin-ichi Sakakibara

    PLOS Genetics   18 ( 10 ) e1010438 - e1010438  2022.10  [Refereed]

    Authorship:Lead author

     View Summary

    The actin filament is a fundamental part of the cytoskeleton defining cell morphology and regulating various physiological processes, including filopodia formation and dendritic spinogenesis of neurons. Serine/threonine-protein kinase Pak4, an essential effector, links Rho GTPases to control actin polymerization. Previously, we identified the Inka2 gene, a novel mammalian protein exhibiting sequence similarity to Inka1, which serves as a possible inhibitor for Pak4. Although Inka2 is dominantly expressed in the nervous system and involved in focal-adhesion dynamics, its molecular role remains unclear. Here, we found that Inka2-iBox directly binds to Pak4 catalytic domain to suppress actin polymerization. Inka2 promoted actin depolymerization and inhibited the formation of cellular protrusion caused by Pak4 activation. We further generated the conditional knockout mice of the Inka2 gene. The beta-galactosidase reporter indicated the preferential Inka2 expression in the dorsal forebrain neurons. Cortical pyramidal neurons of Inka2-/- mice exhibited decreased density and aberrant morphology of dendritic spines with marked activation/phosphorylation of downstream molecules of Pak4 signal cascade, including LIMK and Cofilin. These results uncovered the unexpected function of endogenous Pak4 inhibitor in neurons. Unlike Inka1, Inka2 is a critical mediator for actin reorganization required for dendritic spine development.

    DOI

    Scopus

    1
    Citation
    (Scopus)
  • Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development

    Seiya Yamada, Ayaka Sato, Naotada Ishihara, Hiroki Akiyama, Shin-ichi Sakakibara

    iScience   24 ( 12 ) 103484 - 103484  2021.12  [Refereed]  [International journal]

    Authorship:Lead author

     View Summary

    Brain development is a highly orchestrated process requiring spatiotemporally regulated mitochondrial dynamics. Drp1, a key molecule in the mitochondrial fission machinery, undergoes various post-translational modifications including conjugation to the small ubiquitin-like modifier (SUMO). However, the functional significance of SUMOylation/deSUMOylation on Drp1 remains controversial. SUMO-specific protease 5 (Senp5L) catalyzes the deSUMOylation of Drp1. We revealed that a splicing variant of Senp5L, Senp5S, which lacks peptidase activity, prevents deSUMOylation of Drp1 by competing against other Senps. The altered SUMOylation level of Drp1 induced by Senp5L/5S affects mitochondrial morphology probably through controlling Drp1 ubiquitination and tubulation of the endoplasmic reticulum. A dynamic SUMOylation/deSUMOylation balance controls neuronal polarization and migration during the development of the cerebral cortex. These findings suggest a novel role of post-translational modification, in which deSUMOylation enzyme isoforms competitively regulate mitochondrial dynamics via Drp1 SUMOylation levels, in a tightly controlled process of neuronal differentiation and corticogenesis.

    DOI PubMed

    Scopus

    7
    Citation
    (Scopus)
  • Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex

    Seiya Yamada, Ayaka Sato, Shin-ichi Sakakibara

    iScience   23 ( 5 ) 101058 - 101058  2020.05  [Refereed]  [International journal]

    Authorship:Lead author

     View Summary

    Engagement of neural stem/progenitor cells (NSPCs) into proper neuronal differentiation requires the spatiotemporally regulated generation of metabolites. Purines are essential building blocks for many signaling molecules. Enzymes that catalyze de novo purine synthesis are assembled as a huge multienzyme complex called "purinosome." However, there is no evidence of the formation or physiological function of the purinosome in the brain. Here, we showed that a signal transduction ATPases with numerous domains (STAND) protein, NACHT and WD repeat domain-containing 1 (Nwd1), interacted with Paics, a purine-synthesizing enzyme, to regulate purinosome assembly in NSPCs. Altered Nwd1 expression affected purinosome formation and induced the mitotic exit and premature differentiation of NSPCs, repressing neuronal migration and periventricular heterotopia. Overexpression/knockdown of Paics or Fgams, other purinosome enzymes, in the developing brain resulted in a phenocopy of Nwd1 defects. These findings indicate that strict regulation of purinosome assembly/disassembly is crucial for maintaining NSPCs and corticogenesis.

    DOI PubMed

    Scopus

    12
    Citation
    (Scopus)
  • Control of cell migration by the novel protein phosphatase-2A interacting protein inka2

    Hiroki Akiyama, Yumi Iwasaki, Seiya Yamada, Hiroyuki Kamiguchi, Shin-ichi Sakakibara

    Cell and Tissue Research   380 ( 3 ) 527 - 537  2020.01  [Refereed]  [International journal]

     View Summary

    Cell migration is essential for many physiological and pathological processes, including embryonic development, wound healing, immune response and cancer metastasis. Inka2 transcripts are observed in migrating cells during embryonic development, suggesting the involvement of inka2 in cell migration. However, its precise role remains unclear. Here, we found that inka2 controlled focal adhesion dynamics and cell migration, likely by regulating protein phosphatase-2A (PP2A) function. A scratch assay revealed that inka2 shRNA-transfected NIH3T3 cells showed rapid wound closure, indicating an inhibitory effect by inka2 on cell migration. Live-cell imaging of NIH3T3 cells expressing EGFP-paxillin using total internal reflection fluorescence microscopy revealed that inka2 knockdown increased the turnover rate of focal adhesions. Given that PP2A, which consists of catalytic (C), regulatory (B) and scaffolding (A) subunits, is known to regulate focal adhesions, we examined the inka2-PP2A interaction. Immunoprecipitation revealed an association between inka2 and the PP2A C subunit. Binding of Inka2 to the C subunit prevented the association between the A and C subunits, suggesting that inka2 can inhibit PP2A function. Furthermore, both inka2 expression and PP2A inhibition decreased focal adhesion kinase-paxillin interaction, resulting in reduced formation of focal adhesions. We assessed the effect of pharmacological PP2A inhibition on the inka2 knockdown-induced increase in cell migration speed and found that treatment with a PP2A inhibitor negated the accelerated migration of inka2 knockdown cells. These results suggest that inka2 knockdown exerts its effects through PP2A-dependent regulation of focal adhesions. Our findings contribute to a better understanding of the molecular mechanisms underlying cell migration.

    DOI PubMed

    Scopus

    10
    Citation
    (Scopus)
  • Expression profile of the STAND protein Nwd1 in the developing and mature mouse central nervous system

    Seiya Yamada, Shin-ichi Sakakibara

    Journal of Comparative Neurology (with cover page)   526 ( 13 ) 2099 - 2114  2018.09  [Refereed]  [International journal]

    Authorship:Lead author

     View Summary

    The orchestrated events required during brain development, as well as the maintenance of adult neuronal plasticity, highly depend on the accurate responses of neuronal cells to various cellular stress or environmental stimuli. Recent studies have defined a previously unrecognized, broad class of multidomain proteins, designated as signal transduction ATPases with numerous domains (STAND), which comprises a large number of proteins, including the apoptotic peptidase activating factor 1 (Apaf1) and nucleotide-binding oligomerization domain-like receptors (NLRs), central players in cell death and innate immune responses, respectively. Although the involvement of STANDs in the central nervous system (CNS) has been postulated in terms of neuronal development and function, it remains largely unclear. Here, we identified Nwd1 (NACHT and WD repeat domain-containing protein 1), as a novel STAND protein, expressed in neural stem/progenitor cells (NSPCs). Structurally, Nwd1 was most analogous to the apoptosis regulator Apaf1, also involved in mitosis and axonal outgrowth regulation in the CNS. Using a specific antibody, we show that, during the embryonic and postnatal period, Nwd1 is expressed in nestin-positive NSPCs in vivo and in vitro, while postnatally it is found in terminally differentiated neurons and blood vessels. At the subcellular level, we demonstrate that Nwd1 is preferentially located in the cytosolic compartment of cultured NSPCs, partially overlapping with cytochrome c. These observations imply that Nwd1 might be involved in the neuronal lineage as a new STAND gene, including having a pro-apoptotic or nonapoptotic role, similar to Apaf1.

    DOI PubMed

    Scopus

    9
    Citation
    (Scopus)

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Presentations

  • 脳発生を制御する新たな分子機構

    山田 晴也

    早稲田大学 人間科学総合研究センター 生命科学系シンポジウム 

    Presentation date: 2019.11

  • The novel gene Nwd1 regulate cerebral cortex development

    Seiya Yamada, Hiroki Akiyama, Shin-ichi Sakakibara

    Presentation date: 2019.07

    Event date:
    2019.07
     
     

Research Projects

  • Radmis遺伝子による小頭症発症機構の解明

    日本学術振興会  科学研究費助成事業 若手研究

    Project Year :

    2023.04
    -
    2026.03
     

    山田 晴也

  • 神経発生過程におけるプリン新生・再利用経路の機能解析

    公益財団法人痛風・尿酸財団  2022年度研究助成

    Project Year :

    2022.12
    -
    2024.03
     

    山田晴也, 榊原伸一

  • Nwd1遺伝子によるプリノソーム形成を介した新たな神経分化機構の解明

    日本学術振興会  科学研究費助成事業 研究活動スタート支援

    Project Year :

    2021.08
    -
    2023.03
     

    山田 晴也

  • プリノソーム形成を制御する新たな 因子 Nwd1 を介するプリン代謝調節機構の解明

    公益財団法人痛風・尿酸財団  2020年度研究助成

    Project Year :

    2020.12
    -
    2022.03
     

    榊原伸一, 山田晴也

  • Nwd1遺伝子によるプリノソーム形成を介した新たな大脳皮質発生機構の解明

    日本学術振興会  特別研究員DC1

    Project Year :

    2020.04
    -
    2021.03
     

    山田晴也

 

Syllabus

Teaching Experience

  • Open school

    Waseda University  

    2022.04
    -
    Now
     

  • 現代の生命科学

    早稲田高等学院  

    2022.04
    -
    Now
     

  • ブレインサイエンス

    早稲田大学  

    2022.04
    -
    Now
     

  • ライフサイエンス研究法

    早稲田大学  

    2022.04
    -
    Now
     

  • ライフサイエンス入門(生体システム)

    早稲田大学  

    2022.04
    -
    Now
     

 

Internal Special Research Projects

  • Nwd1遺伝子欠失マウスの遺伝子発現解析

    2022   榊原伸一

     View Summary

    近年、プリン新生のための連続的な酵素反応は、巨大タンパク質複合体「プリノソーム」で行われることが示された。プリノソームの形成は、細胞の増殖や分化に必須の役割を持つと推定されるが、その分子メカニズムの多くは未だ不明である。本研究では、プリノソーム形成に関与する遺伝子Nwd1が制御する分子メカニズムを明らかにするため、Nwd1欠失マウスを作製し、Nwd1欠失マウスを用いた網羅的な遺伝子発現解析を行った。その結果、Nwd1欠失マウスでは約150遺伝子に変動が見られ、Nwd1による分子メカニズム解明への足がかりとなった。本研究はプリン代謝が関連する多様な疾患解明の緒になると考える。

  • プリノソーム形成に関与する新規遺伝子Nwd1のリガンド探索

    2021   榊原伸一

     View Summary

    正常な脳発達においてプリン代謝産物の産生量の制御は不可欠である。近年、プリン新生のための連続的な酵素反応は、巨大タンパク質複合体プリノソームで行われることが示された。プリノソームの形成は、細胞の増殖に必須の役割を持つと推定されるが、プリノソーム形成の分子メカニズムは不明である。申請者が新規同定したNwd1は神経幹細胞で豊富に発現し、プリノソーム形成に関与することで増殖を制御する。本研究ではプリノソーム形成におけるNwd1の役割を明らかにするため、Nwd1と相互作用するタンパク質群の同定を試みた。プルダウンアッセイとLC-MS解析の結果から、Nwd1と相互作用する因子群の同定に成功した。