2022/08/17 更新

写真a

ニシカワ ヨウヘイ
西川 洋平
所属
研究院(研究機関) ナノ・ライフ創新研究機構
職名
次席研究員(研究院講師)

学内研究所等

  • 2021年
    -
    2022年

    リサーチイノベ オープンイノベーション推進セクション   兼任センター員

学位

  • 2019年03月   早稲田大学   工学 (Engineering)

経歴

  • 2021年04月
    -
    継続中

    早稲田大学   ナノ・ライフ創新研究機構   次席研究員

  • 2019年04月
    -
    2021年03月

    国立研究開発法人産業技術総合研究所   産総研・早大 生体システムビッグデータ解析オープンイノベーションラボラトリ

  • 2016年04月
    -
    2019年03月

    独立行政法人日本学術振興会   特別研究員(DC1)

 

研究分野

  • 応用微生物学

論文

  • Strain-level profiling of viable microbial community by selective single-cell genome sequencing

    Masahito Hosokawa, Taruho Endoh, Kazuma Kamata, Koji Arikawa, Yohei Nishikawa, Masato Kogawa, Tatsuya Saeki, Takuya Yoda, Haruko Takeyama

    Scientific Reports   12 ( 1 )  2022年12月  [査読有り]

    DOI

  • Male-killing-associated bacteriophage WO identified from comparisons of Wolbachia endosymbionts of Homona magnanima

    Hiroshi Arai, Hisashi Anbutsu, Yohei Nishikawa, Masato Kogawa, Kazuo Ishii, Masahito Hosokawa, Shiou-Ruei Lin, Masatoshi Ueda, Madoka Nakai, Yasuhisa Kunimi, Toshiyuki Harumoto, Daisuke Kageyama, Haruko Takeyama, Maki N. Inoue

       2022年06月

    担当区分:筆頭著者

    DOI

  • Identification of Lipolytic Enzymes Using High-Throughput Single-cell Screening and Sorting of a Metagenomic Library

    Amani Alma’abadi, Hayedeh Behzad, Mohammed Alarawi, David Conchouso, Yoshimoto Saito, Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama, Katsuhiko Mineta, Takashi Gojobori

    New Biotechnology    2022年05月  [査読有り]

    DOI

  • Targeted single-cell genomics reveals novel host adaptation strategies of the symbiotic bacteria Endozoicomonas in Acropora tenuis coral

    Keigo Ide, Yohei Nishikawa, Toru Maruyama, Yuko Tsukada, Masato Kogawa, Hiroki Takeda, Haruka Ito, Ryota Wagatsuma, Rimi Miyaoka, Yoshikatsu Nakano, Koji Kinjo, Michihiro Ito, Masahito Hosokawa, Kei Yura, Shoichiro Suda, Haruko Takeyama

       2022年04月

    担当区分:筆頭著者

    DOI

  • Revealing within-species diversity in uncultured human gut bacteria with single-cell long-read sequencing

    Masato Kogawa, Yohei Nishikawa, Tatsuya Saeki, Takuya Yoda, Koji Arikawa, Haruko Takeyama, Masahito Hosokawa

       2022年03月

    DOI

  • Single-cell metabolite detection and genomics reveals uncultivated talented producer

    Masato Kogawa, Rimi Miyaoka, Franziska Hemmerling, Masahiro Ando, Kei Yura, Keigo Ide, Yohei Nishikawa, Masahito Hosokawa, Yuji Ise, Jackson K B Cahn, Kentaro Takada, Shigeki Matsunaga, Tetsushi Mori, Jörn Piel, Haruko Takeyama

    PNAS Nexus   1 ( 1 )  2022年03月  [査読有り]

    DOI

  • Integration of Droplet Microfluidic Tools for Single-cell Functional Metagenomics: An Engineering Head Start

    David Conchouso, Amani Al-Ma'abadi, Hayedeh Behzad, Mohammed Alarawi, Masahito Hosokawa, Yohei Nishikawa, Haruko Takeyama, Katsuhiko Mineta, Takashi Gojobori

    Genomics, Proteomics & Bioinformatics    2021年12月  [査読有り]

    DOI

  • MBC2019: Marine Biotechnology Conference 2019.

    Haruko Takeyama, Hiroshi Saito, Yohei Nishikawa

    Marine biotechnology (New York, N.Y.)   22 ( 6 ) 725 - 726  2020年12月  [国際誌]

    DOI PubMed

  • High-Quality Draft Genome Sequence of a Rickettsiales Bacterium Found in Acropora tenuis Coral from Okinawa, Japan.

    Keigo Ide, Yohei Nishikawa, Masato Kogawa, Eisuke Iwamoto, Ashok Zachariah Samuel, Yoshikatsu Nakano, Haruko Takeyama

    Microbiology resource announcements   9 ( 48 )  2020年11月  [国際誌]

    担当区分:筆頭著者

     概要を見る

    Rickettsiales-like organisms are important for the survival and functioning of corals, prompting an investigation of their complete genomes. Earlier reports of the genomes of these organisms remain incomplete. Here, we report a novel draft genome of Rickettsiales bacterial strain SESOKO1, found in Acropora tenuis coral, using single-cell genome technology.

    DOI PubMed

  • Draft Genome Sequence of Okeania sp. Strain KiyG1, Assembled from Single-Amplified Genomes Collected from Cape Kiyan, Okinawa, Japan.

    Muhammad Wahyudin Lewaru, Yohei Nishikawa, Keigo Ide, Masato Kogawa, Masahito Hosokawa, Ashok Zachariah Samuel, Shinpei Sumimoto, Handung Nuryadi, Shoichiro Suda, Haruko Takeyama

    Microbiology resource announcements   9 ( 46 )  2020年11月  [国際誌]

    担当区分:筆頭著者

     概要を見る

    The genus Okeania is a globally distributed group of microorganisms that live in shallow seabed regions. These organisms play several environmentally important roles and are also known producers of several active secondary metabolites with potential human applications. Here, we present a draft genome of Okeania sp. strain KiyG1 (92.7% completeness) that was assembled from four single-amplified genomes.

    DOI PubMed

  • Single-cell genomics of uncultured bacteria reveals dietary fiber responders in the mouse gut microbiota.

    Rieka Chijiiwa, Masahito Hosokawa, Masato Kogawa, Yohei Nishikawa, Keigo Ide, Chikako Sakanashi, Kai Takahashi, Haruko Takeyama

    Microbiome   8 ( 1 ) 5 - 5  2020年01月  [査読有り]  [国際誌]

     概要を見る

    BACKGROUND: The gut microbiota can have dramatic effects on host metabolism; however, current genomic strategies for uncultured bacteria have several limitations that hinder their ability to identify responders to metabolic changes in the microbiota. In this study, we describe a novel single-cell genomic sequencing technique that can identify metabolic responders at the species level without the need for reference genomes, and apply this method to identify bacterial responders to an inulin-based diet in the mouse gut microbiota. RESULTS: Inulin-feeding changed the mouse fecal microbiome composition to increase Bacteroides spp., resulting in the production of abundant succinate in the mouse intestine. Using our massively parallel single-cell genome sequencing technique, named SAG-gel platform, we obtained 346 single-amplified genomes (SAGs) from mouse gut microbes before and after dietary inulin supplementation. After quality control, the SAGs were classified as 267 bacteria, spanning 2 phyla, 4 classes, 7 orders, and 14 families, and 31 different strains of SAGs were graded as high- and medium-quality draft genomes. From these, we have successfully obtained the genomes of the dominant inulin-responders, Bacteroides spp., and identified their polysaccharide utilization loci and their specific metabolic pathways for succinate production. CONCLUSIONS: Our single-cell genomics approach generated a massive amount of SAGs, enabling a functional analysis of uncultured bacteria in the intestinal microbiome. This enabled us to estimate metabolic lineages involved in the bacterial fermentation of dietary fiber and metabolic outcomes such as short-chain fatty acid production in the intestinal environment based on the fibers ingested. The technique allows the in-depth isolation and characterization of uncultured bacteria with specific functions in the microbiota and could be exploited to improve human and animal health. Video abstract.

    DOI PubMed

  • High-throughput identification of peptide agonists against GPCRs by co-culture of mammalian reporter cells and peptide-secreting yeast cells using droplet microfluidics.

    Kenshi Yaginuma, Wataru Aoki, Natsuko Miura, Yuta Ohtani, Shunsuke Aburaya, Masato Kogawa, Yohei Nishikawa, Masahito Hosokawa, Haruko Takeyama, Mitsuyoshi Ueda

    Scientific reports   9 ( 1 ) 10920 - 10920  2019年07月  [査読有り]  [国際誌]

     概要を見る

    Since G-protein coupled receptors (GPCRs) are linked to various diseases, screening of functional ligands against GPCRs is vital for drug discovery. In the present study, we developed a high-throughput functional cell-based assay by combining human culture cells producing a GPCR, yeast cells secreting randomized peptide ligands, and a droplet microfluidic device. We constructed a reporter human cell line that emits fluorescence in response to the activation of human glucagon-like peptide-1 receptor (hGLP1R). We then constructed a yeast library secreting an agonist of hGLP1R or randomized peptide ligands. We demonstrated that high-throughput identification of functional ligands against hGLP1R could be performed by co-culturing the reporter cells and the yeast cells in droplets. We identified functional ligands, one of which had higher activity than that of an original sequence. The result suggests that our system could facilitate the discovery of functional peptide ligands of GPCRs.

    DOI PubMed

  • Correction to: A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro.

    Kazutaka Terahara, Ryutaro Iwabuchi, Masahito Hosokawa, Yohei Nishikawa, Haruko Takeyama, Yoshimasa Takahashi, Yasuko Tsunetsugu-Yokota

    BMC research notes   12 ( 1 ) 322 - 322  2019年06月  [査読有り]  [国際誌]

     概要を見る

    After publication of the original article [1], the authors became aware of a miscalculation in the original Fig. 2d.

    DOI PubMed

  • A CCR5+ memory subset within HIV-1-infected primary resting CD4+ T cells is permissive for replication-competent, latently infected viruses in vitro.

    Kazutaka Terahara, Ryutaro Iwabuchi, Masahito Hosokawa, Yohei Nishikawa, Haruko Takeyama, Yoshimasa Takahashi, Yasuko Tsunetsugu-Yokota

    BMC research notes   12 ( 1 ) 242 - 242  2019年04月  [査読有り]  [国際誌]

     概要を見る

    OBJECTIVE: Resting CD4+ T cells are major reservoirs of latent HIV-1 infection, and may be formed during the early phase of the infection. Although CCR5-tropic (R5) HIV-1 is highly transmissible during the early phase, newly infected individuals have usually been exposed to a mixture of R5 and CXCR4-tropic (X4) viruses, and X4 viral DNA is also detectable in the host. Our aim was to identify which subsets of resting CD4+ T cells contribute to forming the latent reservoir in the presence of both X4 and R5 viruses. RESULTS: Primary resting CD4+ naïve T (TN) cells, CCR5- memory T (TM) cells, and CCR5+ TM cells isolated by flow cytometry were infected simultaneously with X4 and R5 HIV-1, which harbored different reporter genes, and were cultured in the resting condition. Flow cytometry at 3 days post-infection demonstrated that X4 HIV-1+ cells were present in all three subsets of cells, whereas R5 HIV-1+ cells were present preferentially in CCR5+ TM cells, but not in TN cells. Following CD3/CD28-mediated activation at 3 days post-infection, numbers of R5 HIV-1+ cells and X4 HIV-1+ cells increased significantly only in the CCR5+ TM subset, suggesting that it provides a major reservoir of replication-competent, latently infected viruses.

    DOI PubMed

  • Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes.

    Masato Kogawa, Masahito Hosokawa, Yohei Nishikawa, Kazuki Mori, Haruko Takeyama

    Scientific reports   8 ( 1 ) 2059 - 2059  2018年02月  [査読有り]  [国際誌]

     概要を見る

    Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences. Here, we describe Cleaning and Co-assembly of a Single-Cell Amplified Genome (ccSAG), a novel analytical workflow to obtain composite single-cell genomes with elimination of sequence errors. By the integration of ccSAG with a massively parallel single-cell genome amplification platform based on droplet microfluidics, we can generate multiple SAGs and effectively integrate them into the composite genomes quality equivalent to the data obtained from bulk DNA. We obtained two novel draft genomes from single gut microbial cells with high completeness (>96.6%) and extremely low contamination (<1.25%). Moreover, we revealed the presence of single nucleotide polymorphisms in the specific gene by sequence comparison at the single-cell level. Thus, the workflow yields near-complete genomes from uncultured microbes, and enables analyses of genetic heterogeneity within identical strains.

    DOI PubMed

  • Analysis of environmental bacteria at single-cell level

    Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama

    TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems     634 - 637  2017年07月  [査読有り]

     概要を見る

    Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.

    DOI

  • Massively parallel whole genome amplification for single-cell sequencing using droplet microfluidics.

    Masahito Hosokawa, Yohei Nishikawa, Masato Kogawa, Haruko Takeyama

    Scientific reports   7 ( 1 ) 5199 - 5199  2017年07月  [査読有り]  [国際誌]

     概要を見る

    Massively parallel single-cell genome sequencing is required to further understand genetic diversities in complex biological systems. Whole genome amplification (WGA) is the first step for single-cell sequencing, but its throughput and accuracy are insufficient in conventional reaction platforms. Here, we introduce single droplet multiple displacement amplification (sd-MDA), a method that enables massively parallel amplification of single cell genomes while maintaining sequence accuracy and specificity. Tens of thousands of single cells are compartmentalized in millions of picoliter droplets and then subjected to lysis and WGA by passive droplet fusion in microfluidic channels. Because single cells are isolated in compartments, their genomes are amplified to saturation without contamination. This enables the high-throughput acquisition of contamination-free and cell specific sequence reads from single cells (21,000 single-cells/h), resulting in enhancement of the sequence data quality compared to conventional methods. This method allowed WGA of both single bacterial cells and human cancer cells. The obtained sequencing coverage rivals those of conventional techniques with superior sequence quality. In addition, we also demonstrate de novo assembly of uncultured soil bacteria and obtain draft genomes from single cell sequencing. This sd-MDA is promising for flexible and scalable use in single-cell sequencing.

    DOI PubMed

  • Droplet-based microfluidics for high-throughput screening of a metagenomic library for isolation of microbial enzymes.

    Masahito Hosokawa, Yuri Hoshino, Yohei Nishikawa, Tomotada Hirose, Dong Hyun Yoon, Tetsushi Mori, Tetsushi Sekiguchi, Shuichi Shoji, Haruko Takeyama

    Biosensors & bioelectronics   67   379 - 85  2015年05月  [査読有り]  [国際誌]

     概要を見る

    This paper proposes a high-throughput, function-based screening approach of a metagenomic library for isolating novel microbial enzymes by droplet-based microfluidics. We used gel microdroplets (GMDs) dispersed in oil as picoliter-volume reaction vessels for lipolytic enzyme by encapsulating cells in individual GMDs. Using this approach, we monitored the growth of individual cells encapsulated in GMDs and assessed the enzyme reaction activities at the level of an individual GMD. We then applied this method to screen lipolytic enzyme genes from the metagenomic library constructed from soil collected from a quercus serrate forest of Mount Tsukuba, Ibaraki, Japan. In the workflow presented in this study, metagenomic library clones were encapsulated in 100-pL GMDs with a fluorogenic reporter substrate. A total of 67,000 metagenomic library clones can be screened in only 24 h with reduced consumption of reagents (i.e., <10 μL). As a result, we identified a novel lipolytic enzyme, EstT1, belonging to the EstD2 family of esterases and containing a putative signal peptide, which facilitates enzyme export and catalyzation of substrates in the periplasm. Our study demonstrates the potential of microfluidic GMDs as an efficient tool for metagenomic library screening of industrially relevant enzymes with the potential of significantly reducing the cost and time factors involved in successful practical application of microbial enzymes.

    DOI PubMed

  • Monodisperse Picoliter Droplets for Low-Bias and Contamination-Free Reactions in Single-Cell Whole Genome Amplification.

    Yohei Nishikawa, Masahito Hosokawa, Toru Maruyama, Keisuke Yamagishi, Tetsushi Mori, Haruko Takeyama

    PloS one   10 ( 9 ) e0138733  2015年  [査読有り]  [国際誌]

     概要を見る

    Whole genome amplification (WGA) is essential for obtaining genome sequences from single bacterial cells because the quantity of template DNA contained in a single cell is very low. Multiple displacement amplification (MDA), using Phi29 DNA polymerase and random primers, is the most widely used method for single-cell WGA. However, single-cell MDA usually results in uneven genome coverage because of amplification bias, background amplification of contaminating DNA, and formation of chimeras by linking of non-contiguous chromosomal regions. Here, we present a novel MDA method, termed droplet MDA, that minimizes amplification bias and amplification of contaminants by using picoliter-sized droplets for compartmentalized WGA reactions. Extracted DNA fragments from a lysed cell in MDA mixture are divided into 105 droplets (67 pL) within minutes via flow through simple microfluidic channels. Compartmentalized genome fragments can be individually amplified in these droplets without the risk of encounter with reagent-borne or environmental contaminants. Following quality assessment of WGA products from single Escherichia coli cells, we showed that droplet MDA minimized unexpected amplification and improved the percentage of genome recovery from 59% to 89%. Our results demonstrate that microfluidic-generated droplets show potential as an efficient tool for effective amplification of low-input DNA for single-cell genomics and greatly reduce the cost and labor investment required for determination of nearly complete genome sequences of uncultured bacteria from environmental samples.

    DOI PubMed

▼全件表示

書籍等出版物

  • シングルセル解析の新たな可能性

    田中 知明( 担当: 分担執筆,  担当範囲: 新規生理活性物質生産菌のハイスループットスクリーニングプラットフォーム構築)

    北隆館  2021年08月

  • 環境細菌のシングルセルゲノム解析 : 微小液滴を用いたゲノム解析手法とその応用例

    西川 洋平, 細川 正人, 小川 雅人, 竹山 春子( 担当: 共著)

    2020年

  • バイオイノベーションに向けて : バイオテクノロジーの新技術からの新しい視点

    植田, 充美( 担当: 分担執筆)

    シーエムシー出版  2019年03月 ISBN: 9784781314112

Misc

受賞

  • 第5回生物工学学生優秀賞(飛翔賞)

    2016年09月   日本生物工学会   マイクロドロップレットによる単一微生物からの効率的な全ゲノム増幅法の開発  

共同研究・競争的資金等の研究課題

  • シングルゲノム情報を用いた水圏ファージ-宿主間の相互作用解析

    国立研究開発法人科学技術振興機構(JST)  戦略的創造研究推進事業(ACT-X)

    研究期間:

    2020年12月
    -
    2023年03月
     

  • 河川水中の薬剤耐性遺伝子の時空間的な伝播・分布を単一細胞全ゲノム情報から解明する

    日本学術振興会  科学研究費助成事業 若手研究

    研究期間:

    2020年04月
    -
    2022年03月
     

    西川 洋平

  • 育種を加速するパスウェイ型シミュレータの開発とバイオデータ連携基盤構築

    研究期間:

    2019年
    -
    2020年03月
     

  • マイクロドロップレットを用いた単一微生物からの生合成遺伝子クラスターの超並列解析

    日本学術振興会  科学研究費助成事業 特別研究員奨励費

    研究期間:

    2016年04月
    -
    2019年03月
     

    西川 洋平

     概要を見る

    環境中の微生物は99%以上が難培養性であり、生物界におけるダークマターと例えられる。難培養微生物が有する遺伝子の詳細な解析には、単一細胞レベルでのゲノム解析が有効であるが、従来までの手法では、多様な細胞種に適用可能なスクリーニング量が得られていなかった。そこで本研究では、環境中の微生物がもつ全ゲノムの情報を単一細胞レベルで解析し、標的微生物が有する遺伝子の情報を効率的に取得する技術の開発を目指す。
    本年度の研究では、前年度に開発した微小液滴内での全ゲノム増幅技術を用いて、得られた増幅産物からの次世代シーケンサによる配列解析を行った。この結果、微小液滴を用いた単一細胞のゲノム増幅技術が、従来法に比べより高精度かつ多数の単一細胞に対して応用が可能な技術であることを実証することができた。以上の結果をまとめ、論文発表を行った。
    本年度はまた、開発した手法の環境微生物への応用を行い、土壌細菌やマウスの腸内細菌などを対象としたゲノム解析に取り組んだ。これにより、土壌細菌からは17個、マウス腸内細菌からは72個の単一細胞由来のゲノム情報が獲得され、得られた情報の約65%が国際基準で定められた指標においてHigh-qualityもしくはMedium-qualityに分類されることが明らかとなった。以上の結果から、環境の異なる様々な条件のサンプルを用いた場合においても、開発した手法によって高品質なゲノム情報が取得可能であることが証明された。

講演・口頭発表等

  • 1細胞・1粒子レベルのゲノム情報から読み解く、環境細菌とファージの相互作用

    西川洋平  [招待有り]

    バイオインフォマティクス相談部会 第五回講演会  

    発表年月: 2022年03月

    開催年月:
    2022年03月
     
     
  • Microfluidic droplet-based single-cell genome sequencing reveals biological diversities of environmental microbiome in the surrounding area of the Red Sea

    Yohei Nishikawa, Masato Kogawa, Masahito Hosokawa, Katsuhiko Mineta, Kai Takahashi, Keigo Ide, Kei Yura, Hayedeh Behzad, Takashi Gojobori, Haruko Takeyama

    The 2020 International Chemical Congress of Pacific Basin Societies (Pacifichem 2020)  

    発表年月: 2021年12月

    開催年月:
    2021年12月
     
     
  • Microfluidic droplet-based single-cell genomics in aquatic and marine environments for revealing microbial and phage diversity.

    Yohei Nishikawa, Masato Kogawa, Masahito Hosokawa, Keigo Ide, Ryota Wagatsuma, Yuko Tsukada, Haruko Takeyama  [招待有り]

    2021 AFOB virtual conference  

    発表年月: 2021年11月

    開催年月:
    2021年11月
     
     
  • 河川水中の細菌・ウイルスを対象とした1細胞・1粒子レベルでのゲノム解析

    西川 洋平, 我妻 竜太, 塚田 祐子, 井手 圭吾, 小川 雅人, 細川 正人, 竹山 春子

    日本水処理生物学会第57回(神奈川)大会  

    発表年月: 2021年10月

    開催年月:
    2021年10月
     
     
  • シングルゲノム情報を用いた 水圏ファージ-宿主間の相互作用解析

    西川洋平

    第73回 日本生物工学会大会  

    発表年月: 2021年10月

    開催年月:
    2021年10月
     
     
  • Single-cell Genomics of River Water Microbiomes for Revealing the Distribution of Mobile Genetic Elements

    Y. Nishikawa, Y. Tsukada, R. Wagatsuma, K. Ide, M. Kogawa, M. Hosokawa, H. Takeyama

    World Microbe Forum 2021  

    発表年月: 2021年06月

    開催年月:
    2021年06月
     
     
  • 水圏環境の細菌・ファージの動態解明に向けたシングルゲノム解析

    西川洋平

    第21回マリンバイオテクノロジー学会大会  

    発表年月: 2021年05月

    開催年月:
    2021年05月
     
     
  • 多摩川に存在する薬剤耐性菌のゲノム情報をシングルセルレベルで解析する

    西川洋平, 小川雅人, 塚田祐子, 井手圭吾, 細川正人, 竹山春子

    第55回日本水環境学会年会  

    発表年月: 2021年03月

  • 紅海周辺の多様な環境微生物を対象とした大規模かつ高精度なシングルセルゲノム解析

    西川洋平, 小川雅人, 細川正人, 峯田克彦, 高橋海, 坂梨千佳子, 由良敬, 井手圭吾, ベザドハイジ, 五條堀孝, 竹山春子

    第43回日本分子生物学会  

    発表年月: 2020年12月

    開催年月:
    2020年12月
     
     
  • マイクロ流体デバイスを用いた環境細菌の高精度かつ一細胞選択的な全ゲノム解析

    西川洋平, 細川正人, 小川雅人, 井手圭吾, 竹山春子

    電気化学会第87回大会  

    発表年月: 2020年03月

  • Development of microfluidic droplet-based whole genome amplification method for single-cell genome analysis of coral commensal bacteria; Endozoicomonas

    西川洋平, 細川正人, 小川雅人, 井手圭吾, 竹山春子

    Marine biotechnology conference 2019  

    発表年月: 2019年09月

  • 微小反応場を活用したサンゴ共在微生物の高精度なシングルセルゲノム解析

    西川洋平, 細川正人, 小川雅人, 井手圭吾, 竹山春子

    シングルセルゲノミクス研究会2019  

    発表年月: 2019年08月

  • 微小液滴作製技術を応用したサンゴ共在微生物の高精度な単一細胞ゲノム解析技術の確立

    西川洋平, 細川正人, 小川雅人, 井手圭吾, 竹山春子

    第71回日本生物工学会大会  

    発表年月: 2019年08月

  • マイクロドロップレットを用いた網羅的な単一細胞ゲノム解析技術の開発

    西川洋平, 細川正人, 小川雅人, 竹山春子

    第18回 産総研・産技連LS-BT合同研究発表会  

    発表年月: 2019年05月

  • Microfluidic droplet-based whole genome amplification toward bacteria single-cell genomics

    Yohei Nishikawa

    ADVANCED TECHNIQUES TO STUDY AND EXPLOIT THE SPONGE AND CORAL MICROBIOMES WORKSHOP  

    発表年月: 2018年11月

  • サンゴ共在微生物の単一細胞ゲノム解析に向けた微小液滴作成技術の応用

    西川 洋平, 細川 正人, 小川 雅人, 竹田 裕貴, 丸山 徹, 竹山春子

    第20回マリンバイオテクノロジー学会  

    発表年月: 2018年05月

  • Droplet microfluidics toward accurate genome sequencing of environmental bacteria at the single-cell level

    日本化学会第98春季年会  

    発表年月: 2018年03月

  • Droplet microfluidics for massively parallel and accurate whole genome amplification of single-cells

    Yohei Nishikawa

    International Symposium for Nano-Biotechnology, Chemical & Medical Biology and Robotics,  

    発表年月: 2018年

  • 微小液滴を用いた単一細胞からの超並列ゲノム解析技術の開発

    第69回日本生物工学会大会  

    発表年月: 2017年09月

  • マイクロ流体デバイスによる高精度な超並列1細胞ゲノム増幅技術の開発

    西川 洋平, 細川 正人, 小川 雅人, 竹山 春子

    NGS現場の会第5回研究会  

    発表年月: 2017年

  • マイクロドロップレットを用いた高精度な超並列1細胞ゲノム解析技術の開発

    西川 洋平, 細川 正人, 小川 雅人, 竹山 春子

    第11回バイオ関連化学シンポジウム  

    発表年月: 2017年

  • 単一微生物からの高精度な全ゲノム解析に向けたマイクロ流体デバイスの活用

    西川洋平  [招待有り]

    日本生物工学会東日本支部第11回学生発表討論会  

    発表年月: 2016年11月

  • マイクロドロップレットによる単一微生物からの効率的な全ゲノム増幅法の開発

    西川洋平

    第4回日本生物工学会東日本支部コロキウム  

    発表年月: 2016年

  • 微小液滴を用いた単一微生物からの低バイアス全ゲノム増幅法の開発

    西川 洋平, 細川 正人, 竹山 春子

    第10回日本ゲノム微生物学会年会  

    発表年月: 2016年

  • Picoliter-sized droplets for low-bias and contamination-free reactions in whole genome amplification of single bacterial cells

    Yohei Nishikawa, Masahito Hosokawa, Masato Kogawa, Haruko Takeyama

    Biosensors2016  

    発表年月: 2016年

  • ピコリットル容量の微小液滴を用いた単一微生物からの網羅的な全ゲノム増幅

    西川洋平, 細川正人, 小川雅人, 竹山春子

    第68回日本生物工学会大会  

    発表年月: 2016年

  • DROPLET MICROFLUIDICS FOR MASSIVELY PARALLEL AND ACCURATE GENOME AMPLIFICATION OF SINGLE CELLS

    Yohei Nishikawa, Masahito Hosokawa, Masato Kogawa, Haruko Takeyama

    International Confrence on single cell Research 2016  

    発表年月: 2016年

  • 単一細胞ゲノム解析に向けた Droplet-based multiple displacement amplification 法の開発

    西川洋平, 細川正人, 竹山春子

    第17回マリンバイオテクノロジー学会  

    発表年月: 2015年05月

  • マイクロ流体デバイスを用いた単一細胞解析に向けたMultiple displacement amplification (MDA) 法の開発

    西川洋平

    第3回日本生物工学会東日本支部コロキウム  

    発表年月: 2015年

  • Picoliter-sized droplets for low bias whole genome amplification of single cell genome

    Nishikawa Yohei, Masahito Hosokawa, Haruko Takeyama

    2015 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2015)  

    発表年月: 2015年

  • マイクロドロップレットを用いた微生物の薬剤感受性評価

    西川洋平, 細川正人, 竹山春子

    第2回日本生物工学会東日本支部コロキウム  

    発表年月: 2014年

  • シングルセルゲノミクスに向けた Droplet-based Multiple Displacement Amplification 法の開発

    西川洋平, 細川正人, 竹山春子

    第4回CSJ化学フェスタ2014  

    発表年月: 2014年

  • 単一細胞封入ドロップレットを用いた微生物の薬剤感受性評価

    西川洋平, 細川正人, 竹山春子

    電気化学学会第81回大会  

    発表年月: 2014年

  • Droplet-based multiple displacement amplification method for single-cell genomics using microfluidic device

    Yohei Nishikawa

    10th International Symposium on Electrochemical Micro & Nanosystem Technologies (EMNT 2014)  

    発表年月: 2014年

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