細川　正人 (ホソカワ　マサヒト)

写真a

所属

理工学術院 大学院先進理工学研究科

職名

准教授（任期付）
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  • 2008年04月
    -
    2010年03月

    東京農工大学   工学府 博士後期課程   生命工学専攻  

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  • 東京農工大学   博士(工学)

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経歴 【 表示 ／ 非表示 】

  • 2021年04月
    -
    継続中

    早稲田大学   理工学術院 先進理工学研究科   准教授

  • 2018年11月
    -
    継続中

    bitBiome株式会社   取締役CSO

  • 2018年09月
    -
    2020年03月

    早稲田大学   理工学術院総合研究所   次席研究員(研究院講師)

  • 2015年10月
    -
    2019年03月

    科学技術振興機構   さきがけ研究者

  • 2014年04月
    -
    2016年03月

    早稲田大学   ナノ理工学研究機構   次席研究員（研究院助教）

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研究分野 【 表示 ／ 非表示 】

  • 応用生物化学

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  • Cortical transcriptome analysis after spinal cord injury reveals the regenerative mechanism of central nervous system in CRMP2 knock-in mice.

    Ayaka Sugeno, Wenhui Piao, Miki Yamazaki, Kiyofumi Takahashi, Koji Arikawa, Hiroko Matsunaga, Masahito Hosokawa, Daisuke Tominaga, Yoshio Goshima, Haruko Takeyama, Toshio Ohshima

    Neural regeneration research   16 ( 7 ) 1258 - 1265  2021年07月  [国際誌]

    　概要を見る

    Recent studies have shown that mutation at Ser522 causes inhibition of collapsin response mediator protein 2 (CRMP2) phosphorylation and induces axon elongation and partial recovery of the lost sensorimotor function after spinal cord injury (SCI). We aimed to reveal the intracellular mechanism in axotomized neurons in the CRMP2 knock-in (CRMP2KI) mouse model by performing transcriptome analysis in mouse sensorimotor cortex using micro-dissection punching system. Prior to that, we analyzed the structural pathophysiology in axotomized or neighboring neurons after SCI and found that somatic atrophy and dendritic spine reduction in sensorimotor cortex were suppressed in CRMP2KI mice. Further analysis of the transcriptome has aided in the identification of four hemoglobin genes Hba-a1, Hba-a2, Hbb-bs, and Hbb-bt that are significantly upregulated in wild-type mice with concomitant upregulation of genes involved in the oxidative phosphorylation and ribosomal pathways after SCI. However, we observed substantial upregulation in channel activity genes and downregulation of genes regulating vesicles, synaptic function, glial cell differentiation in CRMP2KI mice. Moreover, the transcriptome profile of CRMP2KI mice has been discussed wherein energy metabolism and neuronal pathways were found to be differentially regulated. Our results showed that CRMP2KI mice displayed improved SCI pathophysiology not only via microtubule stabilization in neurons, but also possibly via the whole metabolic system in the central nervous system, response changes in glial cells, and synapses. Taken together, we reveal new insights on SCI pathophysiology and the regenerative mechanism of central nervous system by the inhibition of CRMP2 phosphorylation at Ser522. All these experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Waseda University, Japan (2017-A027 approved on March 21, 2017; 2018-A003 approved on March 25, 2018; 2019-A026 approved on March 25, 2019).

    DOI PubMed

  • Draft Genome Sequence of Okeania sp. Strain KiyG1, Assembled from Single-Amplified Genomes Collected from Cape Kiyan, Okinawa, Japan.

    Muhammad Wahyudin Lewaru, Yohei Nishikawa, Keigo Ide, Masato Kogawa, Masahito Hosokawa, Ashok Zachariah Samuel, Shinpei Sumimoto, Handung Nuryadi, Shoichiro Suda, Haruko Takeyama

    Microbiology resource announcements   9 ( 46 )  2020年11月  [国際誌]

    　概要を見る

    The genus Okeania is a globally distributed group of microorganisms that live in shallow seabed regions. These organisms play several environmentally important roles and are also known producers of several active secondary metabolites with potential human applications. Here, we present a draft genome of Okeania sp. strain KiyG1 (92.7% completeness) that was assembled from four single-amplified genomes.

    DOI PubMed

  • Slow-Cycling Cancer Stem Cells Regulate Progression and Chemoresistance in Colon Cancer.

    Daisuke Shiokawa, Hiroaki Sakai, Hirokazu Ohata, Toshiaki Miyazaki, Yusuke Kanda, Shigeki Sekine, Daichi Narushima, Masahito Hosokawa, Mamoru Kato, Yutaka Suzuki, Haruko Takeyama, Hideki Kambara, Hitoshi Nakagama, Koji Okamoto

    Cancer research   80 ( 20 ) 4451 - 4464  2020年10月  [国際誌]

    　概要を見る

    Cancer chemoresistance is often attributed to the presence of cancer stem cell (CSC)-like cells, but whether they are homogeneously chemoresistant remains unclear. We previously showed that in colon tumors, a subpopulation of LGR5+ CSC-like cells driven by TCF1 (TCF7), a Wnt-responsive transcription factor, were responsible for tumorigenicity. Here we demonstrate that the tumorigenic subpopulation of mouse LGR5+ cells exists in a slow-cycling state and identify a unique 22-gene signature that characterizes these slow-cycling CSC. Seven of the signature genes are specifically expressed in slow-cycling LGR5+ cells from xenografted human colon tumors and are upregulated in colon cancer clinical specimens. Among these seven, four genes (APCDD1, NOTUM, PROX1, and SP5) are known to be direct Wnt target genes, and PROX1 was expressed in the invasive fronts of colon tumors. PROX1 was activated by TCF1 to induce CDKN1C and maintain a slow-cycling state in colon cancer organoids. Strikingly, PROX1 was required for recurrent growth after chemotherapeutic treatment, suggesting that inhibition of slow-cycling CSC by targeting the TCF1-PROX1-CDKN1C pathway is an effective strategy to combat refractory colon cancer in combination with conventional chemotherapy. SIGNIFICANCE: These findings illustrate the importance of a slow-cycling CSC subpopulation in colon cancer development and chemoresistance, with potential implications for the identified slow-cycling CSC signatures and the TCF1-PROX1-CDKN1C pathway as therapeutic targets.

    DOI PubMed

  • High-Quality Draft Single-Cell Genome Sequences of Two Gammaproteobacteria Strains Sampled from Soil in a Strawberry Farm.

    Takuya Yoda, Koji Arikawa, Tatsuya Saeki, Ayumi Matsuhashi, Masahito Hosokawa

    Microbiology resource announcements   9 ( 35 )  2020年08月  [国際誌]

    　概要を見る

    Here, we present high-quality draft single-cell genome sequences of Gammaproteobacteria strains BBSC-SA01 and BBSC-SA02, obtained from uncultivated cells of soil in a strawberry farm using the single-cell sequencing platform bit-MAP. These draft genomes putatively represent novel species within Gammaproteobacteria and allow further investigation into the soil microbiome.

    DOI PubMed

  • Rapid inspection method for investigating the heat processing conditions employed for chicken meat using Raman spectroscopy.

    Rimi Miyaoka, Masahiro Ando, Rieko Harada, Hiroyuki Osaka, Ashok Zachariah Samuel, Masahito Hosokawa, Haruko Takeyama

    Journal of bioscience and bioengineering   129 ( 6 ) 700 - 705  2020年06月  [査読有り]  [国内誌]

    　概要を見る

    In Japan, the imports of meat products have been increasing every year. Heat processing of meat is the current standard method for ensuring domestic animal health, particularly in case of meat products from areas where infectious diseases are known to have occurred in domestic animals. The Animal Quarantine Service needs to establish a method that detects the temperature at which the meat has been heat-processed (endpoint temperature) to ensure that the standard protocol is followed at the production location. Here, we developed a Raman spectroscopy and multivariate statistics (viz. multivariate curve resolution (MCR))-based simple and rapid method for accurately estimating the end point temperature. We showed that the temperature-dependent secondary structure modification of proteins can serve as an accurate indicator of the temperature of heat processing. This methodology can be easily automated for effective utilization by someone who is not an expert in spectroscopy. We envisage a wider application of this method in food analysis, although the present research investigated the application of this method in chicken meat heat processing analysis.

    DOI PubMed

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受賞 【 表示 ／ 非表示 】

  • 第5回「バイオインダストリー奨励賞」

    2021年07月   (一財)バイオインダストリー協会  

  • 平成31年度 科学技術分野の文部科学大臣表彰 若手科学者賞

    2019年04月   文部科学省  

    受賞者： 細川 正人

  • イノベーション賞

    2019年03月   科学技術振興機構さきがけ（統合１細胞領域）  

    受賞者： 細川 正人

  • 第96春季年会 優秀講演賞(学術)

    2016年05月   日本化学会  

    受賞者： 細川 正人

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共同研究・競争的資金等の研究課題 【 表示 ／ 非表示 】

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