NAKANISHI, Takuya

写真a

Affiliation

Research Council (Research Organization), Comprehensive Research Organization

Job title

Senior Researcher(Professor)

Education 【 display / non-display

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    1998

    Hokkaido University   Graduate School of Science   Division of Chemistry (Doctoral Course)  

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    1995

    Hokkaido University   Graduate School of Science   Division of Chemistry (Master's Course)  

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    1993

    Hokkaido University   Faculty of Science   Department of Chemistry  

Degree 【 display / non-display

  • Hokkaido University   Doctor of Science

Research Experience 【 display / non-display

  • 2021.04
    -
    Now

    Waseda University   Comprehensive Research Organization   Senior Researcher (Professor)

  • 2019.04
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    2021.03

    Waseda University   Research Organization for Nano & Life Innovation   Guest Senior Researcher (Guest Professor)

  • 2017.11
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    2021.03

    Waseda University Academic Solutions Corporation   Education and Research Consulting Team, Project Planning and Development Dept.   Supervisory Researcher/Consultant

  • 2016.10
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    2019.03

    Waseda University   Research Organization for Nano & Life Innovation   Adjunct Researcher

  • 2016.10
    -
    2017.10

    National Institute of Advanced Industrial Science and Technology   Computational Bio Big-Data Open Innovation Laboratory   Invited Researcher

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Professional Memberships 【 display / non-display

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    The Magnetics Society of Japan

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    The Japan Society of Vacuum and Surface Science

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    The Japan Society of Applied Physics

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    The Chemical Society of Japan

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    The Electrochemical Society of Japan

 

Research Areas 【 display / non-display

  • Functional solid state chemistry

  • Fundamental physical chemistry

  • Nanobioscience

  • Nanomaterials

Research Interests 【 display / non-display

  • Characterization of Nanomaterials

  • Synthesis of Nanomaterials

  • Molecular Recognition

  • Films and Assemblies

  • Electrochemistry

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Papers 【 display / non-display

  • Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.

    Sho Hideshima, Hiroki Hayashi, Hiroshi Hinou, Shunsuke Nambuya, Shigeki Kuroiwa, Takuya Nakanishi, Toshiyuki Momma, Shin-Ichiro Nishimura, Yoshihiro Sakoda, Tetsuya Osaka

    Scientific reports   9 ( 1 ) 11616 - 11616  2019.08  [Refereed]  [International journal]

     View Summary

    Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.

    DOI PubMed

  • Effect of human serum on the electrical detection of amyloid-β fibrils in biological environments using azo-dye immobilized field effect transistor (FET) biosensor

    Sho Hideshima, Shofarul Wustoni, Masumi Kobayashi, Hiroki Hayashi, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

    Sensing and Bio-Sensing Research   17   25 - 29  2018.02  [Refereed]

     View Summary

    As amyloid-β peptide 1–42 (Aβ42) was found to be an emerging and important biomarker for Alzheimer's disease, the detection of this peptide in biological samples such as human serum (HS) has become very important for evaluating the potential disease state and determining the appropriate treatment. In this study, we developed an electrical analysis strategy based on a field effect transistor (FET) biosensor as a simple and reliable technique for confirming the presence of Aβ42 aggregates (fibrils) in biological samples. By utilizing Congo red immobilized on the FET gate surface as a biorecognition element, we observed remarkable sensitivity and specificity for detecting Aβ42 fibrils. Furthermore, we optimized the procedure to minimize the interference of abundant human serum albumin for the detection system using HS samples. The optimized system of Congo red-immobilized FET enables measurement of Aβ42 fibrils in the 100-pM level in HS samples, which is lower than its clinical concentration. The FET device can be applied as a biosensing system for mass and routine screening of peptide biomarkers related to Alzheimer's disease.

    DOI

  • Multianalyte detection of cancer biomarkers in human serum using label-free field effect transistor biosensor

    Ke Si, Shanshan Cheng, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

    Sensors and Materials   30 ( 5 ) 991 - 999  2018  [Refereed]

     View Summary

    Recent statistics show that the incidence and mortality of cancer are on the rise. Among all types of cancer, lung cancer and liver cancer are the most prevalent. We have many biomarkers for these two cancers, among which the cytokeratin fragment 21-1 (CYFRA 21-1) and α-fetoprotein (AFP) are the most common and widely used. Among the various detection methods, the field effect transistor (FET) biosensor is one of the most attractive approaches, providing a label-free, fast, and low-cost electrical detection of biomarkers with high specificity and sensitivity. In previous work, we applied biomarkers for a single cancer type. With the detection of CYFRA 21-1 and neuron-specific enolase (NSE), lung cancer was differentiated in the early stages. Compared with the early work, the biomarkers for the two cancer types, CYFRA 21-1 for lung cancer and AFP for liver cancer, were prepared in this work. The detection of CYFRA 21-1 and AFP in human serum was achieved at the same time with the limits of detection of 1 and 10 ng mL−1, while the cut-off values were 4 and 10 ng mL−1. The method featured short analytical time, small sample volume, and low cost. With its good selectivity and appropriate sensitivity, clinical application will become popular in the future.

    DOI

  • Label-free detection of allergens in food via surfactant-induced signal amplification using a field effect transistor-based biosensor

    Sho Hideshima, Mai Saito, Keisuke Fujita, Yoshitaka Harada, Mika Tsuna, Satoshi Sekiguchi, Shigeki Kuroiwa, Takuya Nakanishi, Tetsuya Osaka

    SENSORS AND ACTUATORS B-CHEMICAL   254   1011 - 1016  2018.01  [Refereed]

     View Summary

    In recent years, food safety concerning food allergy has received increasing attention globally. A simple and sensitive detection method should be developed to measure trace amounts of allergens in foods. Here, we propose a label-free field effect transistor (FET)-based biosensing system for the detection of a buckwheat allergenic protein, BWp16, by surfactant-induced signal amplification. BWp16 could be detected by coupling with an anionic surfactant, sodium dodecyl sulfate (SDS), as this coupling enhanced the net charge of the protein, enough to be detected by FET biosensors. A significant response was observed when the allergen was coupled with SDS, while the responses were decreased or unchanged when it was coupled with a cationic or non-ionic surfactant, suggesting that the SDS coupling maintains the antibody recognition ability of the target allergen, and it would be useful to enhance the sensor responses. The fluorescence spectroscopic measurement revealed that the SDS molecules were successfully coupled with target allergenic protein BWp16, resulting in an increase in its net charge. Furthermore, we demonstrated that the FET biosensor enables specific detection of the allergen in food at the desired concentration levels for food safety analysis, suggesting that it could be used as an alternative food allergen analyzer, both industrially and domestically. (C) 2017 The Authors. Published by Elsevier B.V.

    DOI

  • Effective induction of death in mesothelioma cells with magnetite nanoparticles under an alternating magnetic field

    Shofu Matsuda, Eri Nakajima, Takuya Nakanishi, Airi Hitsuji, Hong Zhang, Akane Tanaka, Hiroshi Matsuda, Toshiyuki Momma, Tetsuya Osaka

    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS   81   90 - 96  2017.12  [Refereed]

     View Summary

    With the objective of finding an avenue for development of magnetic hyperthermia as an effective mesothelioma treatment, the influence of heating by magnetite nanoparticles (MNPs) with a diameter of similar to 40 nm, which were incorporated into cells and then subjected to AC magnetic field, on induction of cell death was investigated in all three histological subtypes of human mesothelioma cells (i.e., epithelioid NCI-H28, sarcomatoid NCI-H2052, and biphasic MSTO-211H cells). Cellular uptake of MNPs was observed in all cell types, but the amount of MNPs incorporated per cell into MSTO-211H cells was smaller than in NCI-H28 and NCI-H2052 cells. On the other hand, cell death induced by cellular uptake of MNPs was observed specifically in MSTO-211H cells. Hence, when cells are heated by intracellular MNPs under AC magnetic field, a high degree of cell mortality in NCI-H28 and NCI-H2052 cells is induced by the temperature increase derived from the high amount of intracellular MNPs, but the combination of intracellular heating and cell-type-specific toxicity of MNPs induced high rates of cell death in MSTO-211H cells even at a lower temperature. Almost all of the heated cells were dead after 24-h incubation at 37 degrees C in all histological subtypes. Additionally, higher mortalities were observed in all three types of mesothelioma cells after MNPs-heating, as compared to the heating with a thermostatic bath. Herein, the significance of cellular uptake of MNPs for effectively inducing cell death in mesothelioma has been demonstrated in vitro. (C) 2017 Elsevier B.V. All rights reserved.

    DOI

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Misc 【 display / non-display

  • ELECTROCHEMICAL MICRO & NANOSYSTEM TECHNOLOGIES Foreword

    Tetsuya Osaka, Takuya Nakanishi

    ELECTROCHIMICA ACTA   183   1 - 2  2015.11

    Other  

    DOI

  • Field Effect Transistor Based Micro Sensors ―pH Sensors and Biosensors―

    Tetsuya Osaka, Shigeki Kuroiwa, Sho Hideshima, Takuya Nakanishi

    Chemical Sensors   28 ( 1 ) 8 - 15  2012.03  [Refereed]

    Article, review, commentary, editorial, etc. (scientific journal)  

    J-GLOBAL

  • Size‐tuned Synthesis of Magnetite Nanoparticles and Their Biomedical Application

    Takuya Nakanishi, Hong Zhang, Tetsuya Osaka

    IEICE Technical Report (Institute of Electronics, Information and Communication Engineers)   110 ( 310(MR2010 34-41) ) 13 - 15  2010.11  [Invited]

    J-GLOBAL

  • Synthesis of magnetic nanoparticles and their application to bioassays

    Tetsuya Osaka, Tadashi Matsunaga, Takuya Nakanishi, Atsushi Arakaki, Daisuke Niwa, Hironori Iida

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY   384 ( 3 ) 593 - 600  2006.02  [Refereed]

    Book review, literature introduction, etc.  

     View Summary

    Magnetic nanoparticles have been attracting much interest as a labeling material in the fields of advanced biological and medical applications such as drug delivery, magnetic resonance imaging, and array-based assaying. In this review, synthesis of iron oxide magnetic nanoparticles via a reverse micelle system and modification of their surface by an organosilane agent are discussed. Furthermore, as a practical biological assay system, the magnetic detection of biomolecular interactions is demonstrated by using the combination of a patterned substrate modified with a self-assembled monolayer and the magnetic nanoparticles.

    DOI PubMed CiNii

  • Construction and Characteristic Functions of Thin Layered Film of Semiconductor‐Nanoparticles. Advance in Organic‐Inorganic Composites.

    Takuya Nakanishi, Bunsho Ohtani

    Shokubai (Catalysts & Catalysis)   40 ( 7 ) 552 - 559  1998.10  [Invited]

    J-GLOBAL

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Industrial Property Rights 【 display / non-display

  • 光学的センサー

    柳沢 雅広, 中西 卓也, 島本 直伸, 齋藤 美紀子, 逢坂 哲彌

    Patent

    J-GLOBAL

  • FePtナノ粒子の製造方法

    逢坂 哲彌, 杉山 敦史, 川北 泉, 中西 卓也, 飯田 広範, 小島 邦彦, 多田 健太郎, 小原 智, 岩城 仁

    Patent

    J-GLOBAL

  • 光学的センサー

    特許第5083781号

    柳沢雅広, 中西卓也, 島本直伸, 齋藤美紀子, 逢坂哲彌

    Patent

    J-GLOBAL

  • 磁気微粒子包含細胞及びその製造方法

    逢坂 哲彌, 飯田 広範, 中西 卓也, 秋山 靖人

    Patent

    J-GLOBAL

  • 磁気微粒子包含細胞及びその製造方法

    特許第5476620号

    逢坂哲彌, 飯田広範, 中西卓也, 秋山靖人

    Patent

    J-GLOBAL

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Awards 【 display / non-display

  • Poster Award, Chirality 2006 - 18th International Symposium on Chirality (ISCD-18)

    2006.06  

  • Best Poster Presentation Award, The 56th Annual Meeting of the International Society of Electrochemistry

    2005.09  

  • Poster Award, Chirality 2003 - 15th International Symposium on Chirality (ISCD-15)

    2003.10  

Research Projects 【 display / non-display

  • 自己組織化単分子膜によるキラル識別とその効果的検出法の開発

    Project Year :

    2010.04
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    2011.03
     

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Presentations 【 display / non-display

  • Nanoscale Design and Preparation of Solid-Liquid Interfaces for Biosensors and Chiral Sensors

     [Invited]

    3rd DGIST-WASEDA Workshop on Electrochemistry 

    Presentation date: 2015.12

  • Synthesis of Magnetite Nanoparticles using Organic Amines and Evaluation for Biomedical Applications

     [Invited]

    The Catholic University of Korea & Waseda University 2nd Joint Symposium 

    Presentation date: 2015.11

  • 電界効果トランジスタを利用したバイオセンサにおける固液ナノ界面

     [Invited]

    グリーンナノテクノロジー電極研究会「固液界面における物理化学と機能性界面物質相構築」ワークショップ 

    Presentation date: 2015.10

  • Design and Preparation of Nano-interfaces and Nano-materials for Biosensing and Biomedical Applications

     [Invited]

    International Symposium on Integration of Chemistry and Bioscience 

    Presentation date: 2014.01

  • 高性能センサが導く健やかな暮らしと社会

     [Invited]

    タスクフォース「未来社会創成の会」第一回フォーラム 

    Presentation date: 2013.11

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Committee Memberships 【 display / non-display

  • 2017.01
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    2017.05

    2017 International Workshop on Electrified Interfaces for Energy Conversions (EIC2017), Organizing Committee Member

  • 2014.12
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    2015.06

    Symposium on Molecular Chirality 2015 (MC2015), Local Committee Member in Executive Committee

  • 2013.03
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    2015.02

    The Electrochemical Society of Japan  Member of Convention Planning Committee

  • 2013.10
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    2014.11

    10th International Symposium on Electrochemical Micro & Nanosystem Technologies (EMNT2014), Secretary General

  • 2012.10
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    2013.07

    12th Conference for BioSignal and Medicine (CBSM2013), Executive Committee Member

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