研究者詳細 - 菊山　榮

写真a

キクヤマ　サカエ

菊山　榮

所属

教育・総合科学学術院

職名

名誉教授

学位

理学博士 ( 東京大学 )

ホームページ

http://www.f.waseda.jp/kikuyama

経歴

1975年

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- 早稲田大学教授
1975年

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- Waseda University, Professor
1969年

-

1975年

早稲田大学助教授
1969年

-

1975年

早稲田大学
1967年

-

1969年

早稲田大学講師
1967年

-

1969年

早稲田大学
1965年

-

1967年

東京大学助手
1965年

-

1967年

Tokyo University, Research Associate
1963年

-

1964年

アルバートアインシュタイン医科大学研究助手
1963年

-

1964年

Albert Einstein College of Medicine,
　

　

　

Research Assistant

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学歴

　

-

1965年

東京大学生物科学研究科動物学
　

-

1965年

東京大学
　

-

1959年

東京大学理学部生物学科動物学課程
　

-

1959年

東京大学

委員歴

2002年

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日本動物学会評議員
2000年

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日本比較内分泌学会会長
1996年

-

2000年

日本動物学会評議員
1998年

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ヨーロッパ比較内分泌学会(European Society for Coparative Endocrinology) 国際学術委員
1998年

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行動神経内分泌学会評議員
1997年

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国際比較内分泌学会連合(International Federation of Comparative Endocrinological Societies) 評議員
1996年

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日本比較生理生化学会評議員
1993年

-

1994年

日本動物学会評議員
1991年

-

1992年

日本動物学会評議員
1988年

-

1989年

日本動物学会評議員

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所属学協会

　

　

　

日仏生物学会
　

　

　

アメリカ化学受容科学会
　

　

　

日本神経行動学会
　

　

　

日本神経内分泌学会
　

　

　

日本比較生理・生化学会
　

　

　

国際神経行動学会
　

　

　

国際行動神経内分泌学会
　

　

　

国際比較内分泌学連合
　

　

　

アジアオセアニア比較内分泌学会
　

　

　

ヨーロッパ比較内分泌学会
　

　

　

日本動物学会
　

　

　

日本比較生理生化学会
　

　

　

日本発生生物学会
　

　

　

日本内分泌学会
　

　

　

アジアオセアニア比較内分泌学会(Asia and Oceania Society for Comparative Endocrinology)
　

　

　

国際神経行動学会(International Society for Neuroethology)
　

　

　

ヨーロッパ比較内分泌学会(European Society for Coparative Endocrinology)
　

　

　

国際比較内分泌学会連合(International Federation of Comparative Endocrinological Societies)
　

　

　

行動神経内分泌学会
　

　

　

日本比較内分泌学会

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研究キーワード

比較内分泌学
生物形態・構造
比較内分泌学比較生理学発生生物学
Comparative endocrinology Comparative physiology Developmental biology Morphology Structureof Organism Comparative Endocrinology

受賞

Zoological Scince Award（日本動物学会論文賞）

2003年
Zoological Science Award

2003年
The Yoshimura Prize（下垂体研究会）

2002年
The Yoshihama Prize

2002年
Zoological Science Award(日本動物学会論文賞)

1997年
Zoological Science Award

1997年
日本動物学会賞

1991年
The Zoological Society Prize

1991年
Zoological Science Award(日本動物学会論文賞)

1988年
Zoological Science Award

1988年

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書籍等出版物

Hormonal regulation of growth in amphibians

"Biology of Amphibians vol. 6: Endocrinology of Amphibians" (H. Heatwale, Ed.), Surrey Beatty and Sons, Chipping Norton (Australia) 2004年

Works(作品等)

カニクイガエル幼生の水,塩及び浸透圧調節(海外学術研究-学術調査)

1988年

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カニクイガエルの水,塩及び浸透圧調節:生理学的,形態学的及び発生学的観点より(海外学術研究-学術調査)

1987年

-

共同研究・競争的資金等の研究課題

両生類の変態

その他の研究制度
両生類のフェロモン

その他の研究制度
両生類下垂体機能の調節

その他の研究制度
視床下部―下垂体系の起源

その他の研究制度
両生類の変態・成長・生殖のホルモン調節

その他の研究制度
間脳視床下部一下垂体の発生・分化

その他の研究制度
両生類有尾目の求愛行動

その他の研究制度
両生類下垂体ホルモンの分泌調節

その他の研究制度
Endocrine control of amphibian metamorphosis, growth and reproduction

The Other Research Programs
Development and differentiation of hypothalamo-hypophyseal system

The Other Research Programs
Courtship behavior of urodeles

The Other Research Programs
Regulation of pituitary hormone secretion in amphibians

The Other Research Programs

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Misc

Molecular cloning of bullfrog prolactin receptor cDNA: changes in prolactin receptor mRNA level during metamorphosis

Hasunuma, I, K Yamamoto, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 138 ( 3 ) 200 - 210 2004年09月

　概要を見る

In amphibian larvae, prolactin (PRL) is known to possess growth-promoting and anti-metamorphic activities. For further understanding of the role of PRL in larvae, bullfrog PRL receptor (bfPRLR) cDNA was obtained from the tail fin of premetamorphic tadpoles by use of the reverse transcription-polymerase chain reaction (RT-PCR) coupled with 5'- and 3'-rapid amplification of cDNA ends (RACE). The predicted bfPRLR was composed of 617 amino acids, contained a single transmembrane domain, and showed 33-57% sequence homologies with known sequences of vertebrate PRLRs. When bfPRLR was transiently expressed, specific binding of I-125-labeled bullfrog PRL (bfPRL) was observed. By Northern blot analysis, a 3-kb transcript was detected in the tail fin. By RTPCR bfPRLR mRNA expression was detected mainly in the brain, kidney, skin, and tail throughout prometamorphic and middle climactic periods. The results of an RNase protection assay revealed that the bfPRLR mRNA level in the tail fin increased around the onset of climax (stage XX) and was maintained at a relatively high value at least until mid-climax (stage XXII). It also revealed that bfPRLR mRNA level in the kidney of larvae gradually rose as metamorphosis progressed. The results support the view that PRL in larval period acts not only on the larval organs but also on the organs that are necessary for the adult life to maintain or develop their structures and functions. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Molecular cloning of bullfrog corticotropin-releasing factor (CRF): effect of homologous CRF on the release of TSH from pituitary cells in vitro

Y Ito, R Okada, H Mochida, H Hayashi, K Yamamoto, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 138 ( 3 ) 218 - 227 2004年09月

　概要を見る

Corticotropin-releasing factor (CRF) plays multiple roles in vertebrate species. In non-mammalian vertebrates, CRF has been considered to be the major thyrotropin (TSH)-releasing factor. This notion, however, was derived from experimental data on CRF of mammalian origin. Moreover, in the case of amphibians it has never been directly proved that CRF stimulates the release of TSH from the pituitary. The presently described experiment was conducted to provide direct evidence that homologous CRF enhances the release of TSH from the bullfrog (Rana catesbeiana) pituitary. First, cloning of cDNA encoding bullfrog CRF (fCRF) was accomplished. The cDNA encoding fCRF precursor was isolated from a cDNA library of the bullfrog hypothalamus. The amino acid sequence of fCRF predicted from the amplified cDNA sequence showed 83 and 95% identities with the sequences of ovine and human CRFs, respectively. An antiserum against the fCRF synthesized on the basis of the amino acid sequence was raised and used for immunohistochemical staining of the hypothalamus-hypophyseal region of the bullfrog brain. It stained some of the cell bodies situated mainly in the preoptic area, the nucleus infundibularis dorsalis and nucleus hypothalamicus ventralis and the axons that terminate in the median eminence and neural lobe. The synthetic fCRF was tested for its TSH-releasing activity toward anterior pituitary cells of adult bullfrogs in an in vitro system. As a result, the fCRF caused the release of TSH from the dispersed pituitary cells into the culture medium concentration-dependently, as measured by a specific radioimmunoassay for bullfrog TSH. The potency of the fCRF was almost equivalent to that of ovine CRF. Human urocortin III (hUCN III), a CRF receptor type 2 (CRF-R2) specific agonist enhanced the release of TSH from the pituitary cells in culture, suggesting the involvement of CRF-R2 in the CRF-induced TSH release in the bullfrogs. Culture of pituitary cells in the presence of the hypothalamic extract (HE) and alpha-helical CRF9-41, a CRF-R antagonist, revealed that the antagonist suppressed the TSH-releasing activity of the HE by approximately 50%, suggesting that endogenous CRF contributes as a TSH-releasing factor. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Processing of multiple forms of preprosodefrin in the abdominal gland of the red-bellied newt Cynops pyrrhogaster: regional and individual differences in preprosodefrin gene expression

T Iwata, JM Conlon, T Nakada, F Toyoda, K Yamamoto, S Kikuyama

PEPTIDES 25 ( 9 ) 1537 - 1543 2004年09月

　概要を見る

Peptides derived from the post-translational processing of preprosodefrin were isolated from an extract of the abdominal glands of male red-bellied newts Cynops pyrrhogaster obtained 5 months prior to the onset of the breeding season. Structural characterization of the peptides showed that the pheromone sodefrin (SIPSKDALLK) is stored in a biologically inactive COOH-terminally extended form (SIPSKDALLKISA). It follows, therefore. that the activation of a protease that cleaves at a Lys-Ile bond to generate the active pheromone must occur by the time of onset of reproductive behavior. Additional peptides (representing preprosodefrin-(146-175)-peptide and preprosodefrin-(159-173)-peptide), that are derived from the precursor by cleavage at monobasic and dibasic processing sites, were also purified from the extract. The isolation of paralogs of these peptides, including an inactive COOH-terminally extended form of [Asn(10)]sodefrin, provides evidence for the expression of multiple genes encoding preprosodefrin. PCR products derived from total RNAs from the abdominal gland of individual newts collected from three different regions of Japan were analyzed. The data confirm the existence of multiple genes encoding sodefrin and its variants whose expression varied according to the individuals and the regions. However, genes encoding sodefrin were found to be expressed in all the specimens sampled. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Peptide pheromones in newts

F Toyoda, K Yamamoto, T Iwata, Hasunuma, I, M Cardinali, G Mosconi, AM Polzonetti-Magni, S Kikuyama

PEPTIDES 25 ( 9 ) 1531 - 1536 2004年09月

書評論文，書評，文献紹介等

　概要を見る

This article reviews the current state of understanding of reproductive pheromones in amphibians, focusing mainly on the purification and characterization of peptide pheromones in newts of the genus Cynops, molecular cloning of cDNAs encoding the pheromone molecules, and hormonal control of secretion of these pheromones. Pheromones that attract sexually developed female Cynops pyrrhogaster and C. ensicauda newts were isolated from the male abdominal glands. The C. pyrrhogaster and C. ensicauda pheromones are peptides, designated sodefrin and silefrin. with the amino acid sequences SIPSKDALLK and SILSKDAQLK, respectively. Each pheromone attracts only conspecific females. Molecular cloning of cDNAs encoding sodefrin and silefrin revealed the presence of precursor proteins that are considered to generate these pheromone peptides. Pheromone precursor mRNA levels and radioimmunoassayable pheromone concentrations in the abdominal glands were elevated by prolactin and androgen. Sexual dimorphism and hormone dependency of the responsiveness of vomeronasal epithelium to sodefrin were noted. Significance of pheromones in the form of peptide for those performing reproductive behavior in an aquatic environment was also discussed. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Possible direct induction by estrogen of calcitonin secretion from ultimobranchial cells in the goldfish

N Suzuki, K Yamamoto, Y Sasayama, T Suzuki, T Kurokawa, A Kambegawa, AK Srivastav, S Hayashi, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 138 ( 2 ) 121 - 127 2004年09月

　概要を見る

The plasma level of calcitonin (CT), a calcium (Ca)-regulating hormone, is known to increase in female teleosts during the reproductive period. In the present study, a correlation between plasma CT and Ca and one between plasma CT and the gonad somatic index were demonstrated in the female goldfish but not in the male. To clarify the relationship between CT and Ca, we examined the plasma CT and Ca levels after injecting immature goldfish with estrogen. At day 1, the plasma CT level significantly increased, whereas the plasma Ca level was not changed from its initial level. This result suggests that the trigger of CT secretion is estrogen and that estrogen directly acts on the ultimobranchial gland (UBG), a CT-secreting organ. To determine whether the UBG is equipped with estrogen receptor (ER), an ER binding assay and immunohistochemical staining of UBG cells with an antibody against ER were conducted. As a result, estrogen-specific binding (K-d, 18.52 nM; B-max, 1.35 pmol/mg protein) and ER-immunoreactivity in the UBG were demonstrated. Furthermore, the expression of alpha, beta, and gamma types of ER in the UBG was also detected by use of the reverse-transcription polymerase chain reaction. Thus, we concluded that estrogen acts on the UBG to induce the release of CT, which in turn plays an important role in reproduction directly and/or indirectly through Ca. This is the first report on the existence of ERs in a teleost UBG and the occurrence of CT secretion caused by estrogen. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Effect of bullfrog LH and FSH on newt testes under different temperatures

S Tanaka, M Sakai, MA Hattori, S Kikuyama, K Wakabayashi, Y Hanaoka

GENERAL AND COMPARATIVE ENDOCRINOLOGY 138 ( 1 ) 1 - 7 2004年08月

　概要を見る

Environmental temperature plays important roles for amphibian gonadal function. In this study, we examined the responses of testicular tissue of adult male newts (Cynops pyrrhogaster) to amphibian gonadotropins both in vitro and in vivo under different temperatures. When minced testes were incubated in vitro at different temperatures (8-37 degreesC) under an atmosphere of 95% O-2-5% CO2 for 3 It with bullfrog luteinizing hormone (LH) or follicle-stimulating hormone (FSH), LH stimulated testosterone production more than FSH. The testosterone production increased as the incubation temperature increased. Hypophysectomized newts were injected with bullfrog LH or FSH and maintained at 8 or 18 degreesC. In the 18 degreesC group, the testicular weight of the hypophysectornized control decreased when compared with that of with the intact control. The testicular weight of the LH-treated hypophysectornized group decreased more than that of the hypophysectornized control, indicating that LH induced the evacuation of mature spermatozoa from the testes of LH-treated hypophysectomized newts. In the FSH-treated newts, the testicular weight was greater than that in the hypophysectomized control, and was maintained at a value similar to that of the intact control. In the 8 degreesC group, there was no significant difference in testicular size among the intact control, hypophysectomized control, and FSH-treated newts. LH strongly induced spermiation as it did at 18 degreesC. The plasma testosterone level in the hypophysectornized newts decreased dramatically, but LH was effective in restoring it. Its effect was more potent at 8 degreesC than at 18 degreesC. On the other hand, FSH did not induce a significant increase in the plasma testosterone levels at either temperatures. The results indicate a temperature-dependent difference in responsiveness of the testis both in vitro and in vivo to LH and FSH. (C) 2004 Elsevier Inc. All rights reserved.

DOI
Detection of sodefrin-responsive cell in the newt vomeronasal epithelium by calcium imaging.

Trends in Comparative Endocrinology 432 - 434 2004年
In vitro effect of bisphenol A on the thyroid hormone receptor gene expression in the Xenopus tail and testis.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds), Asia and Oceania Society for Comparative Endocrinology. 590 - 593 2004年
Molecular cloning of bullfrog corticotropin-releasing hormone cDNA.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds.), Asia and Oceania Society for Comparative Endocrinology. 248 - 250 2004年
Possible existence of processing enzymes generating sodefrin molecule in the abdominal gland.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds), Asia and Oceania Society for Comparative Endocrinology. 426 - 428 2004年
Localization of prolactin receptor in the newt brain.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds.), Asia and Oceania Society for Comparative Endocrinology. 257 - 259 2004年
Localization of orexin-A-like immunoreactivity in prolactin cells in the bullfrog (Rana catesbeiana) pituitary

T Yamamoto, H Suzuki, H Uemura, K Yamamoto, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 135 ( 2 ) 186 - 192 2004年01月

　概要を見る

Immunohistochemical techniques were employed to investigate the distribution of orexin-A-like immunoreactivity in the bullfrog (Rana catesbeiana) pituitary. Orexin-A-immunoreactive cells were scattered throughout the pars distalis. We found that these cells corresponded to the cells immunostained with antiserum against bullfrog prolactin (fPRL). Immunoelectron microscopic analysis indicated that an orexin-A-like substance coexisted with fPRL within secretory granules. Western blot analysis of bullfrog pituitary extract revealed that anti-human orexin-A antiserum labeled two separate bands which were not labeled with anti-fPRL antiserum. The present study has, for the first time, provided evidence of the intragranular colocalization of orexin-A-like and PRL immunoreactivities in the bullfrog pituitary. (C) 2003 Elsevier Inc. All rights reserved.

DOI
Effects of RF-amide peptides on the release of TSH from the bullfrog pituitary.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds), Asia and Oceania Society for Comparative Endocrinology. 298 - 300 2004年
Prolactin induces courtship behavior in the male newts acting centrally.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds), Asia and Oceania Society for Comparative Endocrinology. 46 - 48 2004年
Development of radioimmunoassay for bullfrog thyroid-stimulating hormone (TSH): effects of hypothalamic releasing hormones on the release of TSH from the pituitary in vitro

R Okada, K Yamamoto, A Koda, Y Ito, H Hayashi, S Tanaka, Y Hanaoka, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 135 ( 1 ) 42 - 50 2004年01月

　概要を見る

A bullfrog (Rana catesbeiana) thyroid-stimulating hormone (TSH) beta-subunit (TSHbeta) antiserum was produced by employing a C- terminal peptide synthesized on the basis of the amino acid sequence deduced from bullfrog TSHbeta cDNA. Immunohistochemical studies revealed that the bullfrog adenohypophyseal cells that immunologically reacted with the anti-bullfrog TSHbeta corresponded to those positively stained with an antiserum against human (h) TSHbeta. The antiserum was used for the development of a specific and sensitive radioimmunoassay (RIA) for the measurement of bullfrog TSH. The sensitivity of the RIA was 0.75+/-0.07 ng TSH/100 mul assay buffer. The interassay and intraassay coefficients of variation were 7.6 and 5.3%, respectively. Several dilutions of pituitary homogenates of larval and adult bullfrogs, or medium in which bullfrog pituitary cells were cultured, yielded dose-response curves that were parallel to the standard curve. Bullfrog prolactin, growth hormone, luteinizing hormone, follicle-stimulating hormone, and alpha-subunit derived from glycoprotein hormones did not react in this assay. Immunoassayable TSH in the pituitary culture medium was confirmed to exist in the form of TSHbeta coupled with the alpha-subunit by an immunoprecipitation experiment using the TSHbeta antiserum and an alpha-subunit antiserum. TSH released from pituitary cells into the medium was also confirmed to possess a considerable activity in stimulating the release of thyroxine from the thyroid glands of larval bullfrogs in vitro.
The effects of hypothalamic hormones such as mammalian gonadotropin-releasing hormone (mGnRH), ovine corticotropin-releasing hormone (oCRH), and thyrotropin-releasing hormone (TRH) on the release of TSH by dispersed anterior pituitary cells of the bullfrog larvae and adults were also studied. CRH markedly stimulated the release of TSH from both adult and larval pituitary cells. Both TRH and GnRH moderately stimulated the release of TSH from adult pituitary cells but not from the larval cells. This is the first report on the development of an RIA for amphibian,TSH, which has provided the direct evidence that the release of TSH from the amphibian pituitary is enhanced by the hypothalamic releasing hormones such as CRH, TRH, and GnRH. (C) 2003 Elsevier Inc. All rights reserved.

DOI
Effects of hypothalamic factors of the release of TSH from bullfrog pituitary cells.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, S. Kikuyama, Eds), Asia and Oceania Society for Comparative Endocrinology. 245 - 247 2004年
Otoconin and chitinase: digression from amphibian pituitary research.

“Trends in Comparative Endocrinology” (T. Oishi, K. Tsutsui, S. Tanaka, 6 - 9 2004年
Expression of prolactin receptor mRNA in the abdominal gland of the newt Cynops ensicauda.

Comp. Biochem. Physiol. A 38 70 - 88 2004年
Multihormonal control of vitellogenesis in lower vertebrates.

Int. Rev. Cytol. 239 1 - 46 2004年

DOI
Teratogenic and anti-metamorphic effects of bisphenol A on embryonic and larval Xenopus laevis

S Iwamuro, M Sakakibara, M Terao, A Ozawa, C Kurobe, T Shigeura, M Kato, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 133 ( 2 ) 189 - 198 2003年09月

　概要を見る

Effects of bisphenol A (BPA) on embryonic and larval development were investigated. In Xenopus laevis blastulae treated with 2.5-3.0 x 10(-5) M BPA or with 10(-5) M 17beta estradiol (E-2), malformation of the head region, scoliosis (curved vertebrate), and suppression of organogenesis were observed. In addition, 10(-5)-10(-4) M BPA blocked tri-iodothyronine (T-3)-inducible resorption of the tail segments from premetamorphic (stage 52-54) larvae in vitro. When stage 52 tadpoles were immersed in 1.0-2.5 x 10(-5) M BPA, deceleration of both spontaneous and thyroxin (T-4)-induced metamorphic changes occurred. Furthermore, BPA suppressed thyroid hormone receptor (TR.) beta gene expression both in vivo and in vitro. Thus, we concluded that BPA at the concentrations examined affects both embryonic development and larval metamorphosis. (C) 2003 Elsevier Science (USA). All rights reserved.

DOI
Occurrence of β-endorphin binding sites in the pituitary of the frog Rana esculenta: effect of β-endorphin on luteinizing hormone secretion.

Gen. Comp. Endocrinol. 132 ( 3 ) 391 - 398 2003年07月

DOI
Postmetamorphic changes in parvalbumin expression in the hindlimb skeletal muscle of the bullfrog, Rana catesbeiana

T Hasebe, K Umezawa, M Sugita, T Iwata, K Yamamoto, T Obinata, S Kikuyama

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS 1646 ( 1-2 ) 42 - 48 2003年03月

　概要を見る

Anuran amphibians, animals that spend a terrestrial life after metamorphosis, exhibit a marked development of hindlimbs during and after metamorphosis. In order to see whether changes occur in the muscle protein components in the course of postmetamorphic development, we subjected gastrocnemius muscle extracts from growing froglets to two-dimensional electrophoresis (2DE). As a result, we found two proteins to undergo a change in level. One spot, indicating a molecular mass of approximately 12 kDa and an isoelectric point (pI) of 5.0 first became detectable at 45 days after metamorphosis. Another spot, corresponding to a protein of 11 kDa and pI 4.8, was prominent until the former spot appeared. N-terminal amino acid sequence analysis and comparison of the spots with those of parvalbumin (PA) revealed that these two proteins were PAalpha and PAbeta. Northern blot analysis using PAalpha and PAbeta cDNAs as probes revealed that the PAbeta mRNA level declined whereas that of PAalpha mRNA rose as the frogs grew. (C) 2002 Elsevier Science B.V All rights reserved.

DOI
Occurrence of prohormone convertase-like substances in the neural complex cells of the ascidian Halocynthia roretzi

G Kawahara, K Terakado, S Tanaka, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 131 ( 1 ) 32 - 37 2003年03月

　概要を見る

Our previous study on the distribution of adrenocorticotropin (ACTH)-like substances in the neural complex (cerebral ganglion, dorsal strand, and neural gland) of an ascidian Halocynthia roretzi revealed that some of the cells in the cerebral ganglion and the cells scattered along the dorsal strand were immunopositive with antiserum against ACTH. In order to ascertain whether these cells are equipped with prohormone convertases, we performed immunohistochemical studies on the neural complex by using antisera against PC1 and PC2. A considerable number of cells around the dorsal strand and a few cells in the neural ganglion were immunopositive with PC1 and/or PC2 antibodies. Immunoelectron microscopic study demonstrated that some granulated cells situated in the cerebral ganglion and along the dorsal strand contained PC1- or PC2-like substances within their secretory granules. Western blot analysis revealed the presence of 66-kDa PC1-like and 70-kDa PC2-like substances in the neural complex. Moreover, immunostaining of consecutive sections showed that the majority of the cells containing PC1- and/or PC2-like substances corresponded to the cells immunoreactive with antisera against ACTH and CLIP but not to those immunoreactive with an antiserum against PRL. Cells belonging to the neural gland neither contained electron-dense granules nor showed immunoreactivity with any antisera employed in this experiment. The possibility that some of the cells situated in the cerebral ganglion and along the dorsal strand are progenitors of vertebrate adenohypophyseal cells is discussed. (C) 2003 Elsevier Science (USA). All rights reserved.

DOI
Hormonal regulation of growth in amphibians.

Biology of Amphibians vol. 6: Endocrinology of Amphibians (H. Heatwale, Ed.)/Surrey Beatty and Sons, Chipping Norton (Australia) pp. 2267-2300 2003年
Endocrinology of reproduction.

Reproductive Biology and Phylogeny of Urodela (D. M. Server, Ed.)/Science Publishers, Enfield, New Hampshire pp. 275-321 2003年
Ghrelin directly interacts with neuropeptide-Y-containing neurons in the rat arcurate cucleus: Ca²⁺ signaling via protein kinase A and N-type channel-dependent mechanisms and cross talk with leptin and orexin.

Diabetes 52; 1-9 2003年
Regional concentration and chromatographic characterization of pituitary adenylate cyclase-activating polypeptide (PACAP) in the brain of the bullfrog, Rana catesbeiana.

Zool. Sci. 20; 1003-1009 2003年

DOI
Expression of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC₁-R) in the ractive astrocytes.

Mol. Brain Res. 115; 10-20 2003年
Involvement of arginine vasotocin in reproductive events in the male newt Cynops pyrrhogaster.

Horm. Behav. 44; 346-353 2003年

DOI
Novel Neuropeptides related to frog growth hormone-releasing peptide: isolation, sequence, and functional analysis.

Endocrinology 144; 3879-3884 2003年

DOI
Molecular cloning of otoconin-22 cDNA in the bullfrog endolymphatic sac: Effect of calcitonin on otoconin mRNA levels.

Endocrinology 144; 3284-3296. 2003年
ATP-binding cassette transporter ABCA2 (ABC2) expression in the developing spinal cord and PNS during myelination

CJ Zhou, N Inagaki, SJ Pleasure, LX Zhao, S Kikuyama, S Shioda

JOURNAL OF COMPARATIVE NEUROLOGY 451 ( 4 ) 334 - 345 2002年09月

　概要を見る

We examined developmental characteristics of the ATP-binding cassette transporter ABCA2 (or ABC2)-expressing cells in rat spinal cord and peripheral nerves. In adult spinal cord, ABCA2 immunoreactivity was detected in lysosome-like organelles of mature oligodendrocyte cell bodies, and a single specific band was detected by Western blot analysis. In postnatal developing spinal cord, ABCA2 immunolabeling was first detected in a small number of cells restricted to the ventral marginal area and the dorsal funiculus at birth (PO). ABCA2-positive cells were immunolabeled by O4, a marker for late progenitor and immature oligodendrocytes. At the same time, myelin basic protein was apparent in the same restricted regions. The number of ABCA2 and O4 co-immunolabeled cells increased quickly in both dorsal and ventral regions from P2 and reached a peak at P8. After transient expression from PO to P8, O4 labeling in white matter tracts decreased and disappeared. In contrast, ABCA2-positive oligodendrocytes persisted in gray and white matter throughout the spinal cord into adulthood. These data suggest a role for the ABCA2 transporter in maturation of oligodendrocyte lineage cells and the onset of myelination in the central nervous system. In addition, ABCA2 immunoreactivity was detected in the ciliated region of the ependyma in the central canal from early postnatal development. ABCA2 immunoreactivity was also detected in the Schwann cell lineage in developing spinal nerves and in adult trigeminal and sciatic nerves. ABCA2 was also expressed in numerous undetermined cells distributed in para-nerve connective tissues and nerve sheaths throughout early postnatal development. These data indicate multiple levels of involvement for ABCA2 in nervous system development especially with strong evidence for a role in myelination. (C) 2002 Wiley-Liss, Inc.

DOI
Hypophyseal development in vertebrates from amphibians to mammals

K Kawamura, T Kouki, G Kawahara, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 126 ( 2 ) 130 - 135 2002年04月

書評論文，書評，文献紹介等

　概要を見る

Classically, it has been widely accepted that epithelial hypophysis develops from the stomodeal ectoderm. This notion was based on purely morphological observation of normal specimens. Recently, evidence is being accumulated that indicates another lineage for the developmental origin of adenohypophysis. This minireview focuses on several series of experimental works that have demonstrated a neuroectodermal origin of the hypophysis in vertebrates from amphibians to mammals. Phylogenetic consideration of the pituitary-like structures of prechordal animals is also presented. (C) 2002 Elsevier Science (USA).

DOI
Environmental Estrogens and reproductive biology in amphibians

G Mosconi, O Carnevali, MF Franzoni, E Cottone, Lutz, I, W Kloas, K Yamamoto, S Kikuyama, AM Polzonetti-Magni

GENERAL AND COMPARATIVE ENDOCRINOLOGY 126 ( 2 ) 125 - 129 2002年04月

書評論文，書評，文献紹介等

　概要を見る

In this paper, the effects of an estrogenic compound, 4-nonyl-phenol (NP), on the amphibians Rana esculenta and Triturus carnifex are described together with those on sexual differentiation in Xenopus laevis. NP increased plasma vitellogenin in male frogs and newts in a dose-related manner; moreover, inhibitory effects on gonadotropin and prolactin (PRL) secretion by pituitary were found together with an elevation of plasma androgens. NP treatment also caused a remarkable increase in number of prolactin-immunolabeled cells, suggesting that xenoestrogen might induce, at least in the newt pituitary, a PRL accumulation possibly due to a reduction of the hormone release. In addition, both NP and bisphenol A caused feminization by increasing the percentage of female phenotypes in X. laevis, and the in vivo effects were more pronounced than those of estradiol-17beta. (C) 2002 Elsevier science (USA).

DOI
Comparative peptidomics of the endocrine pancreas: islet hormones from the clawed frog Xenopus laevis and the red-bellied newt Cynops pyrrhogaster.

J. Endocrinol. 175; 769-777 2002年

DOI
Expression of parvalbumin isoforms in the postmetamorphic bullfrog, Rana catesbeiana.

Proceedings of the 21st Conference of European Comparative Endocrinologists (R. Keller, H. Dircksen, D. Sedlmeier, and H. Vaudry, Eds.)/Monduzzi Editore, Bologna pp. 523-526 2002年
Regional and individual differences in the gene expression of sodefrin variant in the abdominal gland of Cynops pyrrhogaster.

Proceedings of the 21st Conference of European Comparative Endocrinologists(R. Keller, H. Dircksen, D. Sedlmeier, and H. Vaudry, Eds.)/Monduzzi Editore, Bologna pp. 323-325 2002年
Adrenocorticotropin-Like Immunoreactivity in the Granules of Neural Complex Cells of the Ascidian Halocynthia roretzi.

Zool. Sci. 19; 1061-1065 2002年

DOI
Peptide and protein pheromones in amphibians.

Comp. Biochem. Physiol. B-Biochem. Mol. Biol. 132; 69-74 2002年

DOI
A novel amphibian hypothalamic neuropeptide: Isolation, localization, and biological activity.

Endocrinology 143; 411-419 2002年

DOI
Temperature-dependent prolactin secretion and reproductive biology of the newt Triturus carnifex Laur.

Gen. Comp. Endocrinol. 126; 261-268 2002年

DOI
Molecular cloning of a putative gastric chitinase in the toad Bufo japonicus.

Zool. Sci. 19; 293-297 2002年

DOI
Isolation and sequence of a novel amphibian pancreatic chitinase.

Comp. Biochem. Physiol. B-Biochem. Mol. Biol. 132; 381-388 2002年

DOI
Identification of a cDNA encoding a novel amphibian growth hormone-releasing peptide and localization of its transcript.

J. Endocrinol. 174; 395-402 2002年

DOI
Orexin-1 receptor immunoreactivity in chemically identified target neurons in the rat hypothalamus.

Neurosci. Lett. 324; 5-8 2002年

DOI
PACAP and its receptors exert pleiotropic effects in the nervous system by activating multiple signaling pathways.

Curr. Protein Pept. Sci. 3; 423-439 2002年

DOI
Blockade of amphibian metamorphosis by an endocrine disruptor, bisphenol A.

Fourth Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology/Guangzhou, China 2002年
Effects of 4-nonyl-phenol on pituitary lactotrophs of the male urodele amphibian Triturus carnifex

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
The effects of environmental estrogens on the reproductive biology of frog (Rana esculenta) and newt (Triturus carnifex).

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
Effect of an endocrine disruptor, bisphenol A on amphibian metamorphosis.

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
Isolation of a novel pancreatic chitinase and its emergence during anuran metamorphosis.

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
Regional and individual difference in the gene expression of sodefrin variants in the abdominal gland of Cynops pyrrhogaster.

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
Molecular cloning of ascidian prohormone convertase 2.

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
Changes in the expression of parvalbumin isoforms in the hindlimb skeletal muscle during the course of postmetamorphic development of the bullfrog, Rana catesbeiana.

21st Conference of European Comparative Endocrinologists/Bonn, Germany 2002年
プロラクチンは中枢に作用してイモリ求愛行動を誘起する

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
脊椎動物における新規視床下部LPXRFamideペプチドの同定

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
ウシガエル脳中PACAPの生化学的特徴づけ

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
マボヤPC2およびPitx様物質をコードするcDNAのクローニング

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
ウシガエルTSHβ免疫陽性物質の放出に及ぼす視床下部ホルモンの影響

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
ウシガエルプロラクチン受容体のcDNAクローニング

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
ナメクジウオの配偶子および初期胚の凍結保存

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
ウシガエル鰓後腺におけるプロホルモン変換酵素（PC1, PC2）の発現

第27回日本比較内分泌学会大会及びシンポジウム/岡山 2002年
Identification and biological activity of a frog GH-releasing peptide-related peptide.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan (Sep. 24-27, 2002) Vol.19, No. 12; pp. 1484 2002年
Development of novel and sensitive enzyme-immunoassay for PACAP: comparison of brain PACAP concentrations among some vertebrate species.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1484 2002年
Bisphenol A suppresses the elevation of thyroid hormone receptor beta induced by thyroid hormone in Xenopus larvae.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1485 2002年
Direct innervation of the diencephalon by a subset of the primary olfactory neurons.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1485 2002年
Purification and cDNA cloning of a pancreatic chitinase from the bullfrog, Rana catesbeiana.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1486 2002年
Inhibitory effects of estrogen and bisphenol A on thyroxine-induced resorption of bullfrog tadpole tail.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1493 2002年
Two-dimensional electrophoretic profiles of erythrocyte surface proteins in the bullfrog during metamorphic transition.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1494 2002年
Development of radioimmunoassay for bullfrog TSHβ subunit: effect of TRH on the release of immunoassayable TSHβ from the bullfrog pituitary.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1494 2002年
Regulation of otoconin-22 mRNA expression in the bullfrog endolymphatic sac by calcitonin

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1495 2002年
Central action of arginine vasotocin in the induction of courtship behavior of the male newt, Cynops pyrrhogaster.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1495 2002年
Gene expression of sodefrin and its variants in the newt abdominal gland.

Zool. Sci. (Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)/Kanazawa, Japan Vol.19, No. 12; pp. 1495 2002年
Cosecretion of prolactin and growth hormone by dispersed pituitary cells of the adult bullfrog, Rana catesbeiana

A Oguchi, T Aida, A Koda, S Shioda, S Nakajo, T Kobayashi, S Tanaka, K Yamamoto, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 122 ( 1 ) 10 - 16 2001年04月

　概要を見る

The coexistence of prolactin (PRL) and growth hormone (GPI) was previously demonstrated in newly hatched bullfrog (Rana catesbeiana) tadpoles, whereas in adult bullfrogs, there were no cells containing both PRL and GH. However, a cell blot assay with enzymatically dispersed adult pituitary cells demonstrated the existence of cells secreting both PRL and GH. The number of cells secreting both PRL and GH was reduced by a protein synthesis inhibitor, cycloheximide, but not by an RNA synthesis inhibitor, actinomycin D. In situ hybridization and immunostaining of intact pituitary glands revealed the existence of GH mRNA in some of the PRL-immunoreactive cells and of PRL mRNA in some of the GH-immunoreactive cells. We propose that dispersion of the pituitary cells triggered the translation of GH mRNA in the PRL cells and/or of PRL mRNA in the GH cells. (C) 2001 Academic Press.

DOI
Molecular cloning of newt prolactin (PRL) cDNA: Effect of temperature on PRL mRNA expression

N Takahashi, Hasunuma, I, T Iwata, K Umezawa, K Yamamoto, A Marin, Perroteau, I, C Vellano, S Kikuyama

GENERAL AND COMPARATIVE ENDOCRINOLOGY 121 ( 2 ) 188 - 195 2001年02月

　概要を見る

A partial prolactin (PRL) cDNA was specifically PCR amplified from a cDNA library constructed from pituitary mRNAs of the newt (Cynops pyrrhogaster) and cloned into plasmid vectors. One clone thus obtained contained a 739-bp insert encoding the C-terminal amino acid sequence of the mature hormone molecule. Using this clone as a probe, the full-length newt PRL cDNA was screened from the cDNA library. The PRL cDNA clone thus obtained consisted of 1024 bp encoding the entire sequence of the mature PRL molecule in addition to its signal peptide. The amino acid sequence of newt PRL deduced from its nucleotide sequence showed higher homologies with those PRL sequences of tetrapod animals than with those of teleosts. Northern blot analysis revealed the newt PRL mRNA size to be approximately 1 kb. In situ hybridization using the newt PRL cDNA as a probe revealed that the pituitary region expressing PRL mRNA corresponded to that immunoreactive with antiserum against PRL. PRL mRNA levels in the pituitary of newts subjected to room and low temperatures were determined by Northern analysis employing the PRL cDNA as a probe. PRL mRNA levels were significantly higher in the pituitaries of newts subjected to 10 degrees than in those of newts kept at 23 degrees. Likewise, immunoassayable plasma PRL levels were higher in animals subjected to 10 degrees than in those kept at 23 degrees. (C) 2001 Academic Press.

DOI
Developmental origin of the rat adenohypophysis prior to the formation of Rathke's pouch.

Development 128; 959-963 2001年
ATP-binding cassette transporter ABC2/ABCA2 in the rat brain: a novel mammalian lysosome-associated membrane protein and a specific marker for oligodendrocytes but not for myelin-sheaths.

J. Neurosci 21; 849-857 2001年
PACAP activates PKA, PKC and Ca²⁺ signaling cascades in rat neuroepithelial cells.

Peptides 22; 1111-1117 2001年

DOI
Pituitary adenylate cyclase-activating polypeptide and its receptors in amphibians.

Microsc. Res. Tech. 54; 137-157 2001年

DOI
Cloning of prolactin cDNA: Temperature-dependent prolactin mRNA expression.

Perspective in Comparative Endocrinology: Unity and Diversity (H. J. T. Goos, R. K. Rastogi, H. Vaudry, and R. Pierantoni, Eds.)/Monduzzi Editore, Bologna, Italy pp. 121-126 2001年
Endocrine control of reproductive behavior of the newt, Cynops pyrrhogaster.

Perspective in Comparative Endocrinology: Unity and Diversity (H. J. T. Goos, R. K. Rastogi, H. Vaudry, and R. Pierantoni, Eds.)/Monduzzi Editore, Bologna, Italy pp. 317-324 2001年
Effects of hypothalamic factors on the thyroid-stimulating hormone β subunit mRNA expression in the bullfrog pituitary.

Perspective in Comparative Endocrinology: Unity and Diversity (H. J. T. Goos, R. K. Rastogi, H. Vaudry, and R. Pierantoni, Eds.)/Monduzzi Editore, Bologna, Italy pp. 627-632 2001年
Identification and distribution of growth hormone and prolactin cells in pars distalis of Rana tigerina.

Perspective in Comparative Endocrinology: Unity and Diversity (H. J. T. Goos, R. K. Rastogi, H. Vaudry, and R. Pierantoni, Eds.)/Monduzzi Editore, Bologna, Italy pp. 813-819 2001年
Bullfrog ghrelin is modified by n-Octanoic acid at its third threonine residue.

J. Biol. Chem. 276; 40441-40448 2001年

DOI
Application and modification of in situ RT-PCR for detection and cellular localization of PAC1-R splice variant mRNAs in frozen brain sections.

Biotech. Histochem. 76; 75-83 2001年
Endocrine regulation of urodele reproductive behavior.

Recent Advances in Comparative Endocrinology (J. Y. L. Yu, Ed.)/Academia Sinica, Taipei, Taiwan, ROC pp. 1-11 2001年
Effect of activins on pituitary function in the bullfrog, Rana catesbeiana.

Recent Advances in Comparative Endocrinology (J. Y. L. Yu, Ed.)/Academia Sinica, Taipei, Taiwan, ROC pp. 160-166 2001年
Sodefrin-like pheromone in the abdominal gland of Cynops ensicauda: molecular cloning and purification.

Recent Advances in Comparative Endocrinology (J. Y. L. Yu, Ed.)/Academia Sinica, Taipei, Taiwan, ROC pp. 281-287 2001年
Hormonal regulation of synthesis and discharge of a female newt-attracting pheromone, sodefrin.

Association for Chemoreception Sciences XXIIIrd Annual Meeting/Sarasota, USA Abstract; pp. 50 2001年
Studies of the development and function of pituitary gland with toad (Bufo japonicus) embryos and larvae.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 2 2001年
PACAP and its receptors in amphibians: From gene characterization to functional implications.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 1 2001年
Peptides derived from the post-translational processing of prosodefrin in the abdominal gland of the newt, Cynops pyrrhogaster.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 28 2001年
Involvement of arginine vasotocin in the expression of courtship behavior and pheromone and spermatophore discharge in the male newt, Cynops pyrrhogaster.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 29 2001年
Molecular cloning of sodefrin-like peptide cDNA of the newt, Triturus carnifex Laur.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 34 2001年
Environmental estrogens and reproductive biology of the wild population of frog, Rana esculenta.

International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology/Camerino, Italy Abstract; pp. 109 2001年
Endocrine control of reproductive behavior of the newt, Cynops pyrrhogaster>/I>.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract 2001年
Changes in parvalbumin expression in the hindlimb muscle of metamorphosing and postmetamorphic Rana catesbeiana.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract; pp. 97 2001年
Requirement of both thyroid hormone and corticoid for the development of tyrosine hydroxylase-immunoreactive neurons in the rostral preoptic hypothalamus of the toad, Bufo japonicus.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract; pp. 97 2001年
Signal-transduction pathway mediating the action of a newt pheromone, sodefrin.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract; pp. 68 2001年
Cloning of prolactin cDNA: Temperature-dependent prolactin mRNA expression.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract 2001年
Existence of PC1- and PC2-like immunoreactive substances in the ascidian neural complex.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract 2001年
A novel amphibian hypothalamic peptide: Isolation, localization and effect of RFa on the release of bullfrog gonadotropins.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract; pp. 54 2001年
Direct projection of olfactory axons to the posterior hypothalamus.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract; pp. 35 2001年
Effects of hypothalamic factors on the thyroid-stimulating hormone b subunit mRNA expression in the bullfrog pituitary.

14th International Congress of Comparative Endocrinology/Sorrento, Italy Abstract 2001年
マボヤ神経複合体内に存在するPC2様物質

日本動物学会第72回大会/福岡 2001年
ヒキガエル膵臓に存在するキチナーゼ様タンパク質の単離とそのcDNAクローニング

日本動物学会第72回大会/福岡 2001年
アフリカツメガエルの初期発生および変態におよぼすビスフェノールAの影響について

日本動物学会第72回大会/福岡 2001年
ウシガエル亜成体の成長に伴う後肢パルブアルブミン発現の変化

日本動物学会第72回大会/福岡 2001年
ウシガエル下垂体前葉中のTSH b mRNA発現に及ぼす視床下部因子の影響

日本動物学会第72回大会/福岡 2001年
ヒキガエルの体色明化中枢の発達に必要なホルモン条件

日本動物学会第72回大会/福岡 2001年
イモリ雄性ホルモン受容体cDNAクローニングとそのmRNAの発現に及ぼすホルモンの影響

日本動物学会第72回大会/福岡 2001年
イモリ腹腺プロラクチン受容体mRNA発現のホルモン調節

日本動物学会第72回大会/福岡 2001年
ウシガエル視床下部由来の成長ホルモン放出活性をもつ新しいペプチド

日本動物学会第72回大会/福岡 2001年
嗅覚一次ニューロンから間脳に至る直接的神経投射

日本動物学会第72回大会/福岡 2001年
雄イモリ求愛行動とプロラクチンの中枢作用

日本動物学会第72回大会/福岡 2001年
ウシガエル内リンパ嚢における炭酸カルシウム結晶形成に関与するotoconin22の発現調節

日本動物学会第72回大会/福岡 2001年
ソデフリンによるイモリ鋤鼻上皮細胞内カルシウムイオン濃度の変化

日本動物学会第72回大会/福岡 2001年
ヒキガエルpreoptic recess organ前方部の発達とそのホルモン依存性

第26回日本比較内分泌学会大会/東京 2001年
ウシガエル亜成体の成長に伴う後肢筋タンパク質の発現変化

第26回日本比較内分泌学会大会/東京 2001年
マボヤPC2cDNAのクローニング

第26回日本比較内分泌学会大会/東京 2001年
魚類の生殖時におけるカルシトニン（CT）の生理作用

第26回日本比較内分泌学会大会/東京 2001年
両生類の成長ホルモン放出ペプチドのcDNAクローニング

第26回日本比較内分泌学会大会/東京 2001年
イモリの求愛行動発現を引き起こす後葉ホルモンの中枢作用

第26回日本比較内分泌学会大会/東京 2001年
ソデフリンおよびそのバリアントの遺伝子発現

第26回日本比較内分泌学会大会/東京 2001年
ヒキガエル膵臓に特異的なキチナーゼの単離・同定

第26回日本比較内分泌学会大会/東京 2001年
ウシガエルTSHb mRNA発現に及ぼす下垂体ホルモン放出ホルモンの影響

第26回日本比較内分泌学会大会/東京 2001年
アカハライモリ雄性ホルモン受容体cDNAクローニングとそのmRNAの発現に及ぼすホルモンの影響

第26回日本比較内分泌学会大会/東京 2001年
イモリ腹腺内プロラクチン受容体mRNA発現のホルモン調節

第26回日本比較内分泌学会大会/東京 2001年
嗅覚細胞から間脳への直接的神経投射の存在

第26回日本比較内分泌学会大会/東京 2001年
両生類の生殖行動と分子シグナル

行動の分子生物学（山元大輔編）/東京（シュプリンガー・フェアラーク） 2000年
Production of a recombinant newt growth hormone and its application for the development of a radioimmunoassay.

Gen. Comp. Endocrinol. 117; pp.103-116 2000年

DOI
Splice variants of PAC1 receptor during early neural development of rats.

Peptides 21; pp.1177-1183 2000年

DOI
Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR.

Mol. Brain Res. 75; pp.150-158 2000年

DOI
Effect of prolactin and androgen on the expression of a female-attracting pheromone silefrin in the abdominal gland of the newt.

Biol. Reprod. 63; pp.1867-1872 2000年
Hormonal control of urodele reproductive behavior.

Comp. Biochem. Physiol. B 126; pp.221-229 2000年

DOI
Cloning of bullfrog thyroid-stimulating hormone (TSH) β subunit cDNA: expression of TSHβ mRNA during metamorphosis.

Gen. Comp. Endocrinol. 119; pp.224-231 2000年

DOI
Hormonal influence on the olfactory response to a female-attracting pheromone, sodefrin, in the newt, Cynops pyrrhogaster.

Comp. Biochem. Physiol. B 126; pp.239-245 2000年

DOI
Silefrin, a sodefrin-like pheromone in the abdominal gland of the sword-tailed newt, Cynops ensicauda.

FEBS Lett. 472; pp.267-270 2000年

DOI
Distribution of PACAP receptor and its splice variants in the rat brain by in situ RT-PCR.

Ann. N.Y. Acad. Sci. 921; pp.373-374 2000年

DOI
Ontogeny of PACAP receptor and its splice variants in the rat brain.

Ann. N.Y. Acad. Sci. 921; pp.375-376 2000年

DOI
Characterization of the spermiation response, luteinizing hormone release and sperm quality in the American toad (Bufo americanus) and the endangered Wyoming toad (Bufo baxteri).

Reprod. Fertil. Dev. 12; pp.51-58 2000年
Occurrence of immunoreactive activin/inhibin β_B in thyrotropes and gonadotropes in the bullfrog pituitary: possible paracrine/autocrine effects of activin B on gonadotropin secretion.

Gen. Comp. Endocrinol. 118; pp.68-76 2000年

DOI
Effect of activin A and follistatin on the release of pituitary hormones in the bullfrog Rana catesbeiana.

Zool. Sci. 17; pp.971-975 2000年

DOI
Newt prolactin and its involvement in reproduction.

Can. J. Physiol. Pharmacol. 78; pp.984-993 2000年

DOI
Molecular evolution of the growth hormone-releasing hormone/pituitary adenylate cyclase-activating polypeptide gene family. Functional implication in the regulation of growth hormone secretion.

J. Mol. Endocrinol. 25; pp.157-168 2000年
Endocrine regulation of reproductive behavior in the newt Cynops pyrrhogaster.

Zool. Sci. 17; pp.561-570 2000年

DOI
変態最盛期ウシガエル幼生型赤血球の選択的除去機構

第52回日本動物学会関東支部大会/東京 2000年
Silefrin, a female-attracting pheromone in the abdominal grand of the sword-tailed newt, Cynops ensicauda.

XXII Annual Meeting of Associations for Chemoreceptor Sciences/Sarasota Abstract; pp13 2000年
Sodefrin-like pheromone in the abdominal gland of the sword-tailed newt, Cynops ensicauda.

Fourth Congress of the Asia and Oceania Society for Comparative Endocrinology/Taipei Abstract; pp48 2000年
Effect of activin A on the pituitary function in the bullfrog, Rana catesbeiana.

Fourth Congress of the Asia and Oceania Society for Comparative Endocrinology/Taipei Abstract; pp69 2000年
Molecular cloning of pituitary adenylate cyclase-activating polypeptide (PACAP) and its receptor in the frog Rana ridibunda: effects of PACAP and growth hormone-releasing hormone on growth hormone release.

The Endocrine Society's 82nd Annual Meeting/Toronto Abstract; pp166 2000年
Ultrashort neural pathway emanating from the olfactory epithelium and projecting to the posterior hypothalamus.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp50 2000年
Electrophoretic profiles of erythrocyte proteins from the bullfrog before and after metamorphosis.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp58 2000年
Developmental origin of the adenohypophysis in the rat prior to the formation of Rathke's pouch.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp59 2000年
PACAP and its receptor in the frog Rana ridibunda: cloning, distribution and effects of PACAP and GHRH on GH release.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp61 2000年
Molecular cloning of bullfrog TSHβ subunit cDNA: changes in TSHβ mRNA expression during metamorphosis.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp73 2000年
Effect of prolactin and androgen on the expression of a newt pheromone, silefrin.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp105 2000年
Temperature dependent prolactin secretion and its involvement in the seasonal reproductive cycle in Triturus carnifex Laur.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp107 2000年
Silefrin, a sodefrin-like pheromone in the sword-tailed newt, Cynops ensicauda.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp110 2000年
Cloning of sodefrin-like cDNA of the newt, Triturus carnifex Laur.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp96 2000年
Immunohistochemical distribution and ultrastructural localization of ABC2 in the rat brain.

XV International Symposium on Morphological Sciences/Kyoto Abstract; pp97 2000年
マボヤ神経複合体内に存在するPC1、PC2免疫陽性細胞

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp10 2000年
嗅神経の一部は超短絡的に視床下部に向けて投射する

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp10 2000年
ラトケ嚢形成前のラット下垂体原基

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp10 2000年
下垂体に於ける下垂体原基を抗原としたモノクローナル抗体免疫陽性細胞の分布

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp11 2000年
ホルモンにより誘起されるアカハライモリの鋤鼻上皮の形態変化

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp14 2000年
変態期ウシガエル赤血球タンパク質の電気泳動パターンの変化

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp17 2000年
変態期のウシガエル下垂体中POMCmRNAの発現

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp18 2000年
シリフリン発現に及ぼすプロラクチン・雄性ホルモンの効果

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp20 2000年
アクチビンの下垂体前葉細胞機能に及ぼす影響

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp14 2000年
イモリプロラクチンmRNA発現におよぼす温度の影響

日本動物学会第71回大会 (Proceedings of the 71st Annual Meeting of the Zoological Society of Japan)/東京 (Zoological Society of Japan) Abstract; pp5 2000年
シリケンイモリ雌誘引物質シリフリンの発現および分泌に及ぼすホルモンの影響

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp30 2000年
ホルモンにより誘起されるアカハライモリの鋤鼻嗅覚上皮の形態的変化

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp31 2000年
マボヤ神経節および背索にはPC1、PC2様免疫陽性細胞が存在する

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp35 2000年
新しく見いだされた両生類の新規視床下部ペプチド

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp51 2000年
ウシガエル下垂体ホルモン放出に及ぼす影響

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp56 2000年
ラット神経板形成時の腺性下垂体原基

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp57 2000年
ウシガエルの成長ホルモン分泌促進因子・グレリンの構造決定とその特性

第25回日本比較内分泌学会 (Proceedings of the Japan Society for Comparative Endocrinology)/能登 (Japan Society for Comparative Endocrinology) Abstract; pp60 2000年
Identification and cellular localization of ABC2 in the rat CNS: a new marker protein for oligodendrocytes.

Society for Neuroscience's: 30th Annual Meeting/New Orleans Abstract; pp99 2000年
Novel hypothalamic peptide regulating gonadotropin release II. Isolation, localization and function of an amphibian RFa peptide.

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp52 2000年
Origin of the rat adenohypophysis.

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp53 2000年
Existence of possible ancestral pituitary cells in the ascidian neural complex.

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp54 2000年
Olfactory responses to a female-attracting pheromone, sodefrin, in the newt, Cynops pyrrhogaster.

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp66 2000年
A direct neural pathway that connects the olfactory epithelium and the posterior hypothalamus.

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp76 2000年
両生類からみた下垂体の起源

生体調節研究所シンポジウム脳・下垂体・生殖腺/群馬 Abstract; pp2 2000年
両生類有尾目の生殖とフェロモン

日本生化学会関東支部シンポジウム「化学受容研究の最近の進歩」 Abstract; pp6 2000年
Endocrine regulation of urodele reproductive behavior.

Fourth Congress of the Asia and Oceania Society for Comparative Endocrinology/Taipei Abstract; pp3 2000年
両生類のプロラクチンと変態への関与

研究集会予稿集「プロラクチン研究の新展開」 Abstract; pp15 2000年
有尾両生類の生殖行動と下垂体ホルモン

日本下垂体研究会第15回学術集会/岩手 Abstract; pp14 2000年
Local mechanisms regulating frog reproduction.

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp44 2000年
Peptide and protein pheromones in amphibians

20th Conference of European Comparative Endocrinologists/Faro Abstract; pp86 2000年
Expression and transduction of PACAP receptor during brain development

Brain Nose & Pituitary International Symposium/Tokyo Abstract; pp42 2000年
Female newt-attracting activity of sodefrin-related peptides.

日本動物学会第70回大会／山形 pp.20 1999年
Effect of activin B on the function of the bullfrog anterior pituitary.

日本動物学会第70回大会／山形 pp.18 1999年
Effect of arginine vasotocin on the abdominal gland contraction of the male newt, Cynops pyrrhogaster.

日本動物学会第70回大会／山形 pp.17 1999年
Axonal projection to the posterior hypothalamus from the neurons of olfactory origin.

日本動物学会第70回大会／山形 pp.16 1999年
Local effect of prolactin on the proliferation of epidermal cells of the red-bellied newt (Cynops phyrrhogaster).

日本動物学会第70回大会／山形 pp.16 1999年
Immunocytochemical localization of secretory phospholipase A2-like protein obtained from the pituitary gland and its surrounding tissue of the bullfrog, Rana catesbeiana.

日本動物学会第70回大会／山形 pp.16 1999年
Selective removal of larval red blood cells by hepatic and splenic macrophages and changes in the electrophoretic profiles of erythrocyte proteins during metamorphosis of the bullfrog.

日本動物学会第70回大会／山形 pp.11 1999年
Molecular cloning of bullfrog TSH 遵m subunit cDNA.

日本動物学会第70回大会／山形 pp.10 1999年
Isolation of a sodefrin-like pheromone from the abdominal gland of the sword-tailed newt (Cynops ensicauda).

日本動物学会第70回大会／山形 pp.9 1999年
Purification of an antigen molecule of the monoclonal antibody raised against toad pituitary primordium.

日本動物学会第70回大会／山形 pp.8 1999年
Distribution of PACAP receptor and its splice variants in the rat brain by insitu RT-PCR.

4th International Symposium of VIP, PACAP, Glucagon and Related Peptides／Elsinore 83; pp.62 1999年
Ontogeny of PACAP receptor and its splice variants in the rat brain.

4th International Symposium of VIP, PACAP, Glucagon and Related Peptides／Elsinore 83; pp.62 1999年
Effects of neurohypophyseal hormones on reproductive behavior in the newt, Cyops pyrrhogaster.

Fifth International Coongress of Comparative Physiology and Biochemistry／Calgary pp.91 1999年
Analysis of bullfrog POMC-immunoreactive products in two different types of in vitro translation systems.

Fifth International Coongress of Comparative Physiology and Biochemistry／Calgary pp.88 1999年
Newt prolactin and its involvement in reproduction.

Fifth International Coongress of Comparative Physiology and Biochemistry／Calgary pp.20 1999年
ウシガエルPOMC由来ペプチドによる下垂体機能の調節

下垂体研究会第14回学術集会／栃木 pp.19 1999年
雌誘引活性ソデフリンに対する雌イモリの嗅覚応答

神経科学学会／大阪 pp.25 1999年
有尾両生類生殖ホルモンおよびフェロモンによる調節

鋤鼻研究会／能登 pp.7 1999年
Cloning of sodefrin-like peptide cDNA of the sword-tailed newt.

The Association for Chemoception Sciences／Sarasota pp.12 1999年
ウシガエルPOMC関連ペプチドによる下垂体機能の調節

第51回日本動物学会関東支部大会／浦和 pp.13 1999年
ヒキガエルの背地反応調節センターについて

第51回日本動物学会関東支部大会／浦和 pp.13 1999年
Temperature dependent prolactin plasma changes in crested newts, Triturus carnifex.

The Frontiers of the Biology of Amphibia. The International Symposium／Hiroshima pp.70 1999年
Molecular cloning of sodefrin-like peptide cDNA of the newt, Triturus carnifex LAUR.

The Frontiers of the Biology of Amphibia. The International Symposium／Hiroshima pp.69 1999年
Olfactory response to a female-attracting pheromone, sodefrin, in the newt, Cynops pyrrhogaster.

The Frontiers of the Biology of Amphibia. The International Symposium／Hiroshima pp.69 1999年
Hormonal control of urodele reproductive behavior.

The Frontiers of the Biology of Amphibia. The International Symposium／Hiroshima pp.34 1999年
Ontogenic profile of FSH and LH in Rana esculenta.

Gen. Comp. Endocrinol.／Academic Press 116; pp.114-121 1999年

DOI
Enhancement by proopiomelanocortin-derived peptides of growth hormone and prolactin secretion by bullfrog pituitary cells.

Gen. Comp. Endocrinol.／Academic Press 115; pp.101-109 1999年

DOI
Changes of the pituitary proopiomelanocortin mRNA levels during metamorphosis of the bullfrog larvae.

Zool. Sci.／The Zoological Society of Japan 16; pp.255-260 1999年

DOI
Expression of adenohypophyseal hormone genes in metamorphosing bullfrog larvae.

Recent Developments in Comparative Endocrinology and Neurobiology／European Society for Comparative Endocrinology pp.274-276 1999年
Ontogeny and phylogeny of the hypophysis and hypothalamus.

Recent Developments in Comparative Endocrinology and Neurobiology／European Society for Comparative Endocrinology pp.263-266 1999年
Plasma levels of FSH and LH in the frog, Rana esculenta, during larval development.

Recent Developments in Comparative Endocrinology and Neurobiology／European Society for Comparative Endocrinology pp.201-202 1999年
Thyrotropin-immunopositive glandular cells attached to the caudal region of the frog anterior pituitary gland.

Recent Developments in Comparative Endocrinology and Neurobiology／European Society for Comparative Endocrinology pp.197-198 1999年
Identification of novel neuropeptides from the amphibian brain.

Recent Developments in Comparative Endocrinology and Neurobiology／European Society for Comparative Endocrinology pp.171-177 1999年
Effects of proopiomelanocortin (POMC)-derived peptides on pituitary function in the bullfrog, Rana catesbeiana.

Recent Progress in Molecular and Comparative Endocrinology／Hormone Research Center, Chonnam National University pp.503-509 1999年
Involvement of arginine vasotocin in courtship behavior and spermatophore deposition in the male newt, Cynops pyrrhogaster.

Recent Progress in Molecular and Comparative Endocrinology／Hormone Research Center, Chonnam National University pp.201-207 1999年
Rapid and selective removal of larval erythrocytes from the systemic circulation during metamorphosis of the bullfrog Rana catesbeiana.

Develop. Growth Differ.／Blackwell Science 41; pp.639-643 1999年

DOI
Elevation of plasma prolactin concentration by low temperature is the cause of spermatogonial cells death in the newt, Cynops pyrrhogaster.

Gen. Comp. Endocrinol.／Academic Press 113; pp.302-311 1999年

DOI
Olfactory responses to a female-attracting pheromone in the newt, Cynops pyrrhogaster.

Advances in Chemical Signals in Vertebrates／Plenum Publishers pp.607-615 1999年
Female-attracting peptide pheromone in newt cloacal glands.

Advances in Chemical Signals in Vertebrates／Plenum Publishers pp.127-136 1999年
Molecular cloning of newt sex pheromone precursor cDNAs: evidence for the existence of species-specific forms of pheromone.

FEBS Lett.／Elsevier Science 457; pp.400-404 1999年

DOI
The pituitary-skin connection in amphibians. Reciprocal regulation of melanotrope cells and dermal melanocytes

Ann. N.Y. Acad. Sci.／The New York Academy of Sciences 885; pp.41-56 1999年

DOI
Pituitary adenylate cyclase-activating polypeptide receptors during development: expression in the rat embryo at primitive streak stage.

Neuroscience／Elsevier Science Ltd. 93; pp.375-391 1999年

DOI
Prolactin opens the sensitive period for androgen regulation of a larynx-specific myosin heavy chain gene.

J. Neuroendobiol.／John Wiley & Sons Inc. 41; pp.443-451 1999年

DOI
Effects of the two somatostatin variants somatostatin-14 and [Pro(2),Met(13)]somatostatin-14 on receptor binding, adenylyl cyclase activity and growth hormone release from the frog pituitary

L Jeandel, A Okuno, T Kobayashi, S Kikuyama, H Tostivint, Lihrmann, I, N Chartrel, JM Conlon, A Fournier, MC Tonon, H Vaudry

JOURNAL OF NEUROENDOCRINOLOGY 10 ( 3 ) 187 - 192 1998年03月

　概要を見る

Two isoforms of somatostatin from frog brain have been recently characterized, namely somatostatin-14 (SS1) and [Pro(2), Met(13)]somatostatin-14 (SS2). The genes encoding for the precursors of these two somatostatin variants are expressed in hypothalamic nuclei involved in the control of the frog pituitary. The aim of the present study was to investigate the effect of SS1 and SS2 on adenohypophysial cells. Autoradiographic studies using [I-125-Tyr(o), D-Trp(8)] SS1 as a radioligand revealed that somatostatin binding sites are evenly distributed in the frog pars distalis. The SS2 variant was significantly (P < 0.01) more potent than SS1 in competing with the radioligand (IC50=1.2+/-0.2 and 5.6+/-0.6 nM, respectively). Both SS1 and SS2 induced a modest but significant reduction in cAMP formation in dispersed distal robe cells but did not affect spontaneous growth hormone (GH) release. Synthetic human GRF (hGRF) induced a significant increase in cAMP accumulation and GH release in this system. Both SS1 and SS2 inhibited the stimulatory effects of hGRF on cAMP formation and GH secretion. These data show that the SS1 and SS2 variants can regulate adenohypophysial functions. The fact that GH cells are exclusively located in the dorsal area of the frog adenohypophysis, while somatostatin receptors are present throughout the pars distalis, indicates that the two somatostatin isoforms may control the secretion of pituitary hormones additional to GH in amphibians.
Gonadotropins and reproductive function in the anuran amphibian, Rana esculenta

A. M. Polzonetti-Magni, G. Mosconi, O. Carnevali, K. Yamamoto, Y. Hanaoka, S. Kikuyama

Biology of Reproduction 58 ( 1 ) 88 - 93 1998年01月

　概要を見る

In this study, the measurement both of peripheral gonadotropins (FSH and LH) and of sex steroids in male and female wild anuran, Rana esculenta, was performed during the annual reproductive cycle
moreover, the role of gonadotropins in the vitellogenic process and in ovarian steroidogenesis was investigated through in vitro experiments. LH plasma changes in males showed high values during autumn-winter months and during the mating period, when high androgen levels were found. Conversely, for the first time in male vertebrates, a clear correspondence between plasma FSH and estradiol-17β (E2) was shown. In females, FSH peak values were found at the beginning of the mating period in parallel with those of plasma vitellogenin (VTG) and E2
in contrast, high LH levels went together with ovarian weight (gonadosomatic index), which is considered a good marker for the plasma sequestration of VTG by growing oocytes. The in vivo results are corroborated by in vitro studies showing the direct effects of both FSH and LH in inducing hepatic VTG synthesis and release in the culture media. Lastly, although it is not yet known whether or not FSH and LH have separate functions in amphibians, it was clearly shown that they induce ovarian steroid production. These results are discussed in terms of the high seasonality previously demonstrated in this wild frog.

DOI PubMed
”ラトケ前・ラトケ後”－下垂体の発生に関する考察

第13回下垂体研究会学術集会発表抄録集 pp.11 1998年
Effects of Proopiomelanocortin (POMC)-Derived Peptides on Pituitary Function in the Bullfrog, Rana catesbeiana

The Third International Symposium of Asia and Oceania Society for Comparative Endocrinology. Book of Abstracts pp.32 1998年
Involvement of Arginine Vasotocin (AVT) in Courtship Behabior and Spermatophore Deposition in the Newt, Cynops pyrrhogaster

The Third International Symposium of Asia and Oceania Society for Comparative Endocrinology. Book of Abstracts pp.17 1998年
Hormonal Control of Response to and Secretion of Sodefrin in the Red-Bellied Newt

Abstracts of the International Symposium "Current Studies in Reproductive Biology" pp. 1 1998年
Effect of Arginine Vasotocin on Courtship Behavior in the Newt, Cynops pyrrhogaster

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.79 1998年
Hormonal Effect on Olfactory Responses to Sodefrin in the Female Newt,Cynops pyrrhogaster

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.70 1998年
Tyrotropin-Immunopositive Glandular Cells Attached to the Caudal Region of the Frog Anterior Pituitary Gland

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.68 1998年
Cloning of Sodefrin cDNA: Expression of Sodefrin mRNA in the Newt Abdominal Gland

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.34 1998年
Different Electrophoretic Profiles of High Molecular Weight Proteins from Larval and Adult Type Red Blood Cells of the Bullfrog

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.31 1998年
Plasma Levels of FSH and LH in the Frog, Rana esculenta, during Larval Development

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.24 1998年
Possible Paracrine Control of Pituitary Function by Proopiomelanocortin-Derived Peptides in the Bullfrog, Rana catesbeiana

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.1 1998年
Interactions between Lectin and Sugar on the Vomeronasal Epithelium Measured with the Atomic Force Microscope

The Association for Chemoreception Sciences. Book of Abstracts pp.169 1998年
Hormonal Control of Response and Secretion of a Newt Sex Pheromone, Sodefrin

The Association for Chemoreception Sciences. Book of Abstracts pp.137 1998年
Expression of the Bullfrog Adenohypophyseal Hormone Genes during Metamorphosis

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.37 1998年
Ontogeny and Phylogeny of the Hypothalamus-Hypophysis

Abstracts of 19th Conference of European Comparative Endocrinologists/European Society for Comparative Endocrinology pp.36 1998年
Effect of Arginine Vasotocin on Reproductive Behabior in the Newt, Cynops pyrrhogaster

第21回日本神経科学・第41回日本神経化学合同大会 pp.58 1998年
有尾両生類の生殖現象における後葉ホルモンの作用

日本動物学会近畿支部大会アブストラクト pp.18 1998年
雌イモリ誘引物質ソデフリンに対する雌イモリの嗅覚応答

第75回日本生理学会大会アブストラクト pp.98 1998年
Hormonal Control of Reproductive Behavior of the Red-Bellied Newt, Cynops pyrrhogaster

Abstracts of the 4th International Congress of Neuroendocrinology （Folia Endocrinologica Japonica）/The Japan Endocrine Society 74;2,pp.486 1998年
Background Adaptation Center in the Toad, Bufo japonicus

Abstracts of the 4th International Congress of Neuroendocrinology (Folia Endocrinologica Japonica）/The Japan Endocrine Society 74;2,pp.451 1998年
Elevation of Plasma Prolactin Concentrations by Low Temperature Is the Cause of Spermatogonial Cell Death in the Japanese Newt, Cynopspyrrhogaster

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.61 1998年
Involvement of the Foregut Endoderm in the Pituitary Development as Revealed by a Monoclonal Antibody against Toad Pituitary Primordium

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.14 1998年
Changes in the Electrophoretic Pattern of Membrae-Bound Proteins from Red Blood Cells of the Bullfrog During Metamorphosis

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.14 1998年
Role of the Infundibular Primordium in the Development of the Neural Lobe of Hypophysis

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.13 1998年
Effect of Activin, Inhibin and Follistatin on the Release of Gonadotropic Hormone from the Bullfrog Pituitary

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.12 1998年
Possible Involvement of Arginine Vasotocin in Reproductive Events of the Male Newt, Cynops pyrrhogaster

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.12 1998年
Effect of prolactin on proliferative activity of epidermis in the newt, Cynops pyrrhogater by application of bromodeoxyuridine

Proceeding of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/ Zoological Society of Japan pp.11 1998年
Cloning of cDNA Encoding Beta Subunit of Bullfrog Thyroid-Stimulating Hormone

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.11 1998年
Cloning of Sodefrin-Like Peptide cDNA of Sword-Tailed Newt: Expression of The mRNA in the Abdominal Gland

Proseedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan/Zoological Society of Japan pp.11 1998年
Adpituitary Gland: Follicular Structure Bearing Thyrotropin-Immunoreactive Cells Attached to the Caudal Region of the Pars Distalis in the Frog Pituitary Gland

Proseedings of the Japan Society for Comparative Endocrinology/Japan Society for Comparative Endocrinology 13;pp.42 1998年
Hormonal Control of the Reproductive Behavior of the Red-Bellied Newt, Cynops pyrrhogaster

Proseedings of the Japan Society for Comparative Endocrinology/Japan Society for Comparative Endocrinology 13;pp.23 1998年
Prolactin and Stress Response in Frog Rana esculenta

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.639-641 1998年

DOI
Genomic DNA Fragmentation in Red Blood Cells of the Bullfrog (Rana catesbeiana) during Metamorphosis

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.634-635 1998年

DOI
Beta-Endorphin Binding Sites in Pars Distalis of the Frog, Rana esculenta

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.520-521 1998年

DOI
Cosecretion of Prolactin and Growth Hormone by the Pituitary of the Bullfrog

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.453-454 1998年

DOI
Developmental Origin of the Olfactory System and GnRH Neurons in the Amphibia

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.201-204 1998年

DOI
Sodefrin, a Novel Peptide Pheromone in the Newt Cloacal Grand

Annals of the New York Academy of Science/The New York Academy of Science 839;pp.60-65 1998年

DOI
Morphogenesis of the Hypothalamus and Hypophysis: Their Association, Dissociation and Reassociation before and after "Rathke"

Arch. Histol. Cytol. 61;3,pp.189-198 1998年

DOI
Developmental Studies for Identification of the Inhibitory Center of Melanotropes in the Toad, Bufo japonicus

Development Growth and Differentiation/Japanese Society of Developmental Biologists 40;pp.651-658 1998年

DOI
有尾両生類のフェロモン

日本味と匂学会誌 5;pp.15-22 1998年

DOI
Lack of Inhibitory Control of Melanophore-Stimulating Hormone Secretion in the Larval Toad, Bufo japonicus

Zoological Science/Zoological Society of Japan 15;pp.749-752 1998年

DOI
日本動物学会論文賞

1997年
Female-Attracting Peptide Pheromone in Newt Cloacal Glands

Chemical Signals in Vertebrate ;pp9-10 1997年
Hypothalamo-Hypophyseal Complex as a Single Entity: An Ontogenic Viewpoint

Molecular Ancestry of Vertebrate Plypeptide Hormones and Neuropeptides ;pp.10-13 1997年
Olfactory Responses to a Female-Attracting Peptide Pheromone, "Sodefrin" in the Newt, Cynops pyrrhogaster

Chemical Signals in Vertebrates ;pp.29 1997年
Distribution and Ultrastructural Localization of PACAP Receptors in the Rat Pancreatic Islets

Abstract, 3rd International Symposium on VIP, PACAP, and Related Peptides ;pp.20 1997年
Olfactory Responses to a Female-Attracting Peptide, Sodefrin in the Newt, Cynops pyrrhogaster

Buletin de la Societ醇S Franco-Japonase de Biologie ;pp.11 1997年
Simultaneous Secretion of Prolactin and Growth Hormone by the Dispersed Pituitary Cells of the Bullfrog

Zoological Science／Zoological Society of Japan 14;pp.22 1997年
Molecular Cloning of Sodefrin cDNA

Zoological Science／Zoological Society of Japan 14;pp.21 1997年
Paracrine Control of Pituitary Function by Proopiomelanocortine-Derived Peptides in the Bullfrog, Rana catesbeiana

Zoological Science／Zoological Society of Japan 14;pp.21 1997年
Presence of a Prolactin-Like Immunoreactive Substance in the Ascidian Neural Complex

Zoological Science／Zoological Society of Japan 14;pp.20 1997年
Spatio-Temporal Distribution of Reactivity with the Monoclonal Antibody against toad Pituitary Primordium

Zoological Science／Zoological Society of Japan 14;pp.20 1997年
Role of the Adjacent Tissues in the Differentiation of Embryonic Pituitary Cells in Bufo japonicus

Zoological Science／Zoological Society of Japan 14;pp.20 1997年
Application of Recombinant Newt Growth Hormone to the Development of a Homologous Radioimmunoassay

Zoological Science／Zoological Society of Japan 14;pp.19 1997年
Molecular Cloning and Expression of Newt Growth Hormone

Zoological Science／Zoological Society of Japan 14;pp.18 1997年
Olfactory Responses to a Female-Attracting Peptide Pheromone, Sodefrin in the Newt, Cynops pyrrhogaster

Zoological Science／Zoological Society of Japan 14;pp.18 1997年
Hormonally Induced Synaptic Plasticity in Mauthner Cells of Male Red-Bellied Newts

Zoological Science／Zoological Society of Japan 14;pp.17 1997年
Selective Removal of Larval Type Red Blood Cells from Climactic Tadpoles during Bullfrog Metamorphosis

Zoological Science／Zoological Society of Japan 14;pp.16 1997年
Ectopically Transplanted Infundibular Primordium Does Not Induce Formation of the Neurohypophysis and Brain-Adenohypophyseal Conjugation

Zoological Science／Zoological Society of Japan 14;pp.14 1997年
Cloning of Newt Prolactin cDNA

Zoological Science／Zoological Society of Japan 14;pp.5 1997年
プレセニリンIのER膜上での配向解析

第70回日本生化学会大会発表抄録集 ;pp.639 1997年
Age-Associated Changes in Plasma Concentration of Gorwth Hormone (GH), Prolaction (PRL), Thyroid-Stimulating Hormone (TSH) and Thyroxine (T₄) in the Growth-Retarded Mouse

Proceedings of the Japan Society for Comparative Endocrinology／Japan Society for Comparative Endocrinology 22;12,pp.53 1997年
Possible Involvement of Arginine Vasotocin in Reproductive Events of the Male Newt, Cynops pyrrhogaster

Proceedings of the Japan Society for Comparative Endocrinology／Japan Society for Comparative Endocrinology 22;12,pp.21 1997年
Molecular Cloning of Sodefrin-Like Peptide cDNA of the Sword-Tailed Newt (Cynops ensicauda)

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.1747-1750 1997年
Hypophysio-Thyroidal Axis in Growth-Retarded Mice

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.1101-1105 1997年
Stimulation by Proopiomelanocortin-Derived Peptides of GH and PRL Release by Bullfrog Dispersed Anterior Pituitary Cells

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.1033-1037 1997年
Cosecretion of Prolactin and Growth Hormone by the Dispersed PituitaryCells of the Bullfrog Pituitary

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.985-989 1997年
Cloning and Expression of Newt Prolactin Receptor

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.979-983 1997年
Recombinant Newt Growth Hormone and Its Application to Radioimmunoassay

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.921-925 1997年
Distribution of Endothelin 3-Like Immunoreactivity in Bullfrog and Soft-Shelled Turtle Pituitaries

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.789-793 1997年
Role of the Infundibular Primordium in the Development of Adeno- and Neurohypophyses in the Toad

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.781-784 1997年
Production of a Monoclonal Antibody against Primordial Toad Pituitaries

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.775-779 1997年
Role of the Foregut Endoderm in the Differentiation of Pituitary Cells in Bufo japonicus Embryos

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.771-774 1997年
Ascidian Neural Complex Cells Contain Prolactin-Like Immunoreactive Granules

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.755-758 1997年
Expression of Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Receptor Messenger RNA in the Developing Rat Brain by in situ Hybridization

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.671-675 1997年
Molecular Cloning of Two Somatostatin Precursors in the Frog Brain. Differential Localization of the mRNAs and Binding Affinity of the Two Somatostatin Variants

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.661-665 1997年
Changes in the Genomic DNA of Red Blood Cells (RBCs) and Replacement of the RBC Population during Metamorphosis of the Bullfrog

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.393-396 1997年
Possible Involvement of Arginine Vasotocin in Reproductive Events in the Male Newt, Cynops pyrrhogaster

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.365-369 1997年
Plasma Hormonal Changes during the Annual Reproductive Cycle of Rana esculenta Living in a Lagoon

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.361-364 1997年
Newt Sex Pheromone: Hormonal Control of Its Secretion

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.349-354 1997年
Paracrine Factors Influencing LH Release from Frog Pituitary

Advances in Comparative Endocrinology (S. Kawashima and S. Kikuyama eds)／Monduzzi Editore pp.343-347 1997年
Mice Lacking Bombesin Receptor Subtype-3 Develop Metabolic Defects and Obesity

Nature／Macmillan Magazines 390;6656,pp.165-169 1997年

DOI
Stimulation by Proopiomelanocortin-Derived Peptides of LH Release by Bullfrog Dispersed Anterior Pituitary Cells

Zoological Science／Zoological Society of Japan 14;pp.827-831 1997年
Hormonal Alteration of Synaptic Input to Mauthner Cells of the Adult Male Red-Bellied Newts

Zoological Science／Zoological Society of Japan 14;pp.651-657 1997年

DOI
Enhancement by Prolactin of the GnRH-Induced Release of LH from Dispersed Anterior Pituitary Cells of the Bullfrog (Rana catesbeiana)

General and Comparative Endocrinology／Academic Press 107;pp.128-135 1997年

DOI
Female-Attracting Pheromone in Newt Cloacal Glands

Brain Research Bulletin／Elsevier Science 44;4,pp.415-422 1997年

DOI
両生類

新編畜産大事典（田先威和夫監修）／養賢堂 pp.1457-1466 1997年
Localization of Prolactin Cells in Amphibian Pituitary Using the in situ Hybridization Technique

Abstracts of the 7th National Congress of the Italian Society of Neurosciences (Trends in Neurosciences Supplement) 20;9,pp.44 1997年
Distribution of Endothelin 3-Like Immunoreactivity in Gonadotrophs of the Bullfrog (Rana catesbeiana) Pituitary

General and Comparative Endocrinology/Academic Press 107;pp.12-22 1997年

DOI
Prolactin-Like Immunoreactivity in the Granules of Neural Complex Cells in the Ascidian Halocynthia roretzi

Cell & Tissue Research/Springer-Verlag 289;pp.63-71 1997年

DOI
Sodefrin,a novel peptide pheromone in newt cloacal gland.

18th Conference of European Comparative Endocrinologists：From Molecular to Integrative Biology 1996年
Urodele sex pheromone: Hormonal control of its secretion and response to it.

International Symposium on Amphibian Endocrinology 1996年
Female-attracting pheromone in newt cloacal glands.

Vth International Conference on Hormones,Brain and Behavior 1996年
両生類の変態に関わる甲状腺ホルモン以外のホルモン

共同利用シンポジウム動物の変態・現象とその分子機構 1996年
The involvement of reproductive hormones in downstream and upstream migratory behaviors of salmonid fish.

International Symposium of Fish Endocrinology 1996年
Immunohistochemical studies on the localization of activin/inhibin bB in Xenopus pituitary.

International Conference on Inhibin,Activin, Follistatin: Recent Advances and Future Views 1996年
Origin of GnRH cells and olfactory organs in amphibians.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology 1996年
Molecular cloning and nucleotide analysis of newt prolactin and growth hormone cDNAs.

International Symposium on Amphibian Endocrinology 1996年
Gonadotropins and reproductive function in the anuran amphibian,Rana esculenta.

International Symposium on Amphibian Endocrinology 1996年
Possible role of prolactin cells in the regulation of pituitary hormonesecretion in the bullfrog.

Third Congress of the Asia and Oceania Society for Comparative Endocrinology 1996年
アカハライモリの肛門腺腹腺のアンドロゲン受容体に関する免疫組織化学的研究

日本解剖学会 1996年
ヒメマスの母川回帰行動における生殖内分泌系の関与

日本水産学会 1996年
原索動物神経複合体におけるプロラクチン陽性細胞

日本電子顕微鏡学会 1996年
カエル脳に存在する２種のソマトスタチンとその生理活性

日本神経内分泌学会 1996年
Cosecretion of prolactin (PRL) and growth hormone (GH) by the pituitary of the bullfrog.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
Prolactin and stress response in frog Rana esculenta.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
b-endorphin binding sites in pars distalis of frog,Rana esculenta.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
Development and application of a radioimmunoassay for a female-attracting pheromone in the newt,Cynops pyrrhogaster.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
Genomic DNA fragmentation in red blood cells of the bullfrog (Rana catesbeiana) during metamorphosis.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
Role of the foregut endoderm in the development of epithelial hypophysis.

18th Conference of European Comparative Endocrinologists from Molecular to Integrative Biology. 1996年
Hormonal control of responses to a female-attracting pheromone，sodefrin in the newt，Cynops pyrrhogaster.

TOYODA Fumiyo, YAMAMOTO Kazutoshi, TANAKA Sigeyasu, KIKUYAMA Sakae

Proceeding of the Japan Society for Comparative Endocrinology 11 17 - 17 1996年

CiNii
Role of the foregut endoderm and the brain anlagen in the development of epithelial hypophysis.

KAWAMURA Kosuke, KOUKI Tom, KIKUYAMA Sakae

Proceeding of the Japan Society for Comparative Endocrinology 11 11 - 11 1996年

CiNii
Presence of prolactin-like substance in the secretory granules in the neural complex cells of a protochordate，Halocynthia roretzi.

Zoological Science supplement 13 1996年
Morphological study of the olfactory system in the newt,Cynops pyrrhogaster.

Zoological Science supplement 13 1996年
Molecular cloning of a cDNA comparable to sodefrin cDNA in sword-tailed newt (Cynops Ensicauda).

Zoological Science supplement 13 1996年
Radioimmunoassay for a newt sex pheromone,sodefrin: Hormonal influence on the content in the abdominal gland.

Zoological Science supplement 13 1996年
Effects of arginine vasotocin on reproductive events of the male newt，Cynops pyrrhogaster.

Zoological Science supplement 13 1996年
Effect of physical blockage of connection between the primordial hypophysis and the embryonic hypothalamus on the formation of the parsintermedia of pituitary gland in the toad.

Zoological Science supplement 13 1996年
Effect of POMC peptides on the pituitary cell function in the bullfrog，Rana catesbeiana.

Zoological Science supplement 13 1996年
Contact with the foregut endoderm is essential for the development of epithelial hypophysis in bufo.

Zoological Science supplement 13 1996年
Coexistence of PRL- and GH-mRNAs in the bullfrog pituitary.

Zoological Science supplement 13 1996年
Distribution of endothelin 3-like immunoreactivity in the gonadotrophs of the female bullfrog.

Zoological Science supplement 13 1996年
Cloning and nucleotide sequence analysis of urodele prolactin and growth hormone cDNAs.

Zoological Science supplement 13 1996年
Selective removal of larval type red blood cells in metamorphosing bullfrog tadpoles.

Zoological Science supplement 13 1996年
Genomic DNA fragmentation in red blood cells of the bullfrog during metamorphosis.Develop.

Growth Devel Differ. 38,605-615 1996年

DOI
Radioimmunoassay of a newt sex pheromone, sodefrin,and the influence of hormones on its level in the abdominal gland.

Gen. Comp. Endocrinol. 104,356-363 1996年

DOI
Involvement of endogenous prolactin in the expression of courtship behavior in the newt, Cynops Pyrrhogaster.

Gen. Comp. Endocrinol. 102,191-196 1996年

DOI
Release of a-subunit of glycoprotein hormones from the bullfrog pituitary: Possible effect of a-subunit on prolactin cell function.

Gen. Comp. Endocrinol. 102,141-146 1996年

DOI
Occurrence of immunoreactive activin/inhibin bB in gonadotrophs,Thyrotrophs,and somatotrophs of the Xenopus pituitary.

Gen. Comp. Endocrinol. 102,1-10 1996年

DOI
Estrogen receptors in the stingray (Dasyatis akajei) Ultimobranchial gland.

Gen. Comp. Endocrinol. 101,107-114 1996年

DOI
Immunohistochemical analysis of androgen receptor in the abdominal glands of the cloaca of male red-bellied newts, Cynops pyrrhogaster

Zool. Sci. 13,429-433 1996年

DOI
Origin of adenohypophysis and olfactory system in toad: Demonstration by chimera production.

C. R. Soc. Biol. 1996年
内分泌学

朝倉書店 1996年
発生生物学

朝倉書店 1996年
腺性下垂体の起源

日本神経内分泌学会 1995年
イモリのペプチド性フェロモン

脳腸ホルモン学会 10;2 1995年
両生類の生活史の内分泌学

日本内分泌学会 71;6 1995年
Developmental origin of the hypothalamic-hypophyseal system in the amphibian embryo and its relationships to the olfactory primordium

/韓国生物科学協会 1995年
Embryological basis for a novel concept of hypothalamo-hypophyseal system

Abstract of 41th Annual Meeting of the Italian Embryology Group 1995年
Female-attractant from the abdominal gland of the newt, Cynops pyrrhogaster

Abstract of 9th Summer Conference of Sullang Developmental Biology Research Group 1995年
Effect of ablation of presumptive preoptic recess organ on background adaptation in toads

Proceeding of the Japan Society for Comprative Endocrinology 10 1995年
Endogenous prolactin is involved in the expression of courtship behavior in the newt, Cynops pyrrhogaster

KIKUYAMA Sakae, TOYODA Fumiyo, MATSUDA Kouhei, YAMAMOTO Kazutoshi

Proceeding of the Japan Society for Comprative Endocrinology 10 37 - 37 1995年

CiNii
Fragmentation of genomic DNA in red blood cells of the bullfrog during metamorphosis

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Effect of frog brain-derieved somatostatin-related peptides on GH release from the pituitary in vitro

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Possible involvement of endogenous prolactin in the expression of courtship behavior in the male newt (Cynops pyrrhogaster)

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Electrical response of olfactory epithelium to sodefrin in the female newt

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Evidence that male newts (Cynops pyrrhogaster) discharge sodefrin into an encironmental water

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Immunohistochemical analysis of androgen receptor in the abdominal glands of the cloaca of male red-bellie newts

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Radioimmunoassay of bullfrog adrenocorticotropin

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Cosecretion of prolactin (PRL) and growth hormone (GH) by thedispersed pituitary cells of the bullfrog

Zool. Sci./Zoological Society of Japan suppl., v.12 1995年
Hormones and reproductive behavior of amphibians

Clinical Neuroscience 13;130-131 1995年
In vitro development of Xenopus skin glands producing 5-hydroxytryptamine and caerulein

Experientia 51;1040-1044 1995年

DOI
Morphology of the skin glands of the crab-eating frog (Rana cancrivora)

Zool. Sci./Zoological Society of Japan 12, 623-626 1995年
Plasma prolactin levels related to changes of salinity in the green water frog, Rana esculenta

Netherlands J. Zool. 45;53-56 1995年
A sex-attractant in the cloacal gland of the newt

Netherlands J. Zool. 45;160-162 1995年
Hormonal induction of male-like behavior in the female newt, Cynops pyrrhogaster

Zool. Sci./Zoological Society of Japan 12, 815-818 1995年
Sexual activity- dependent changes in Mauthner cell morphology inmale red-bellied newts

J. Neurobiol. 28;126-132 1995年

DOI
Une nouvelle pheromone sexuelle isolee a partir des glandes abdominales du Triton male, Cynops pyrrhogaster

C. R. Soc. Biol. 189;1143-1148 1995年
Growth hormone and prolactin in amphibian reproduction

Zool. Sci./Zoological Society of Japan 12(6);683-694 1995年
Pituitary immunocytochemistry and prolactin plasma levels in hypophysectomized female newts, Triturus carnifex Laur, bearing a long-term pituitary autograft.

Boll. Zool. 62;239-242 1995年
Development and application of a homologous radioimmunoassay for Xenopus prolactin

Gen. Comp. Endocrinol./Academic Press 99;28-34 1995年
Sodefrin: A female-attracting peptide pheromone in newt cloacal glands

Science 267;1643-1645 1995年

DOI
Induction from the posterior hypothalamus is essential for the development of the pituitary proopiomelanocortin (POMC) cells of the toad (Bufo japonicus)

Cell. Tiss. Res. 279;233-239 1995年
Xenopus prolactin: purification, properties and radioimmunoassay

Technology Bulletin/早大理工学研究所 23;19-30 1995年
Chemical signals during courtship of the newt

Human Sci./人間総合研究センタ－ 7;93-100 1995年

▼全件表示

特定課題制度（学内資金）

イモリ雌由来のフェロモンの単離・同定・局在・遺伝子クローニング

2005年

　概要を見る

イモリ雄は求愛行動の開始に先立って雌の総排泄口に鼻先を近づける行動をみせる。これは雌が生殖可能かどうかを判定するためであると推測されている。我々は雌が放出している化学物質によって判定していると想定して、その物質の単離同定を目指した。繁殖期の雌の卵管の抽出物を含むスポンジブロックは雌を有意にひきつける。この抽出物をタンパク分解酵素で処理すると活性を失う。抽出物を分子ふるいにかけると分子量5000以下の画分に活性がみとめられた。したがって活性物質はペプチドであると考えられ、種々のカラムクロマトグラフィーにより活性物質の単離を試み、現在までにアミノ酸3残基よりなる活性ペプチドを得た。このペプチドの抗体を作製し、免疫組織学的に同物質の局在をしらべたところ、総排泄口に近い卵管壁が免疫陽性であることがわかった。同物質をコードするcDNAのクローニングを試みたが、アミノ酸3残基より得られる塩基配列情報では不十分であることがわかった。一方、雄イモリの放出ソデフリン前駆体のcDNAクローンの解析の結果、ソデフリンをコードするmRNAはすべての地方のすべての個体の肛門部腹腺で発現しているが、奈良地方のイモリに特異的にソデフリンとアミノ酸1残基異なるフェロモン様物質を発現していることを発見した。この物質を単離するとともに、同物質を合成し、活性をしらべたところ、奈良産の雌にのみ強い誘引活性を示し、新潟や千葉産イモリの雌には活性を示さないことがわかった。このソデフリンバリアントをアオニリンと名づけた。
新しい脳下垂体調節因子の単離・同定および生理活性

2004年加藤　尚志, 岩田　武男, Hubert Vaudry, 岡田　令子

　概要を見る

　我々は先に広島大学グループとの共同研究により、ウシガエル視床下部より４種のRFアミドペプチド群を単離・同定・生理活性の解析を行った（Koda et al., 2000, Endocrinology 143, 411-419; Sawada et al., 2002, J. Endocrinol. 174, 395-402)が、同様な方法でイモリ脳より上記ウシガエルのものに似たアミノ酸配列を有するペプチドを得、主としてプロラクチン、生長ホルモンの放出活性に焦点をあてて生理活性の解析を行った結果両ホルモンの放出を促進するペプチド、生長ホルモンの放出のみを促進するペプチドの存在を確認した。　一方我々はこれまで困難を極めていた両生類甲状腺刺激ホルモンの放射免疫測定法の開発に成功した。それを用いて、これまで知られている神経ペプチド（哺乳類由来のものも含む）の甲状腺刺激ホルモン放出促進・抑制効果についてのスクリーニングを行った。その結果、LHRHとTRHには幼生下垂体に対しては効果がなく、成体下垂体には弱い放出活性を示すこと、CRF（ヒツジ由来）には幼生、成体下垂体に対して強い放出活性を示すことがわかった。そこでウシガエルCRFをコードするcDNAのクローニングを行い、それから推定されるウシガエルCRFのアミノ酸配列にしたがってペプチドを合成した。それを用いて活性を測定したところ、ヒツジ由来のものと同等の活性をウシガエルCRFに認めた。またCRFによる甲状腺刺激ホルモン放出活性は甲状腺ホルモンによって抑制を受けることも確認された。なお、CRF以外のペプチドでは、アミノ酸配列がCRFに似ているsauvagineに放出活性がみとめられた。更にsomatostatinには抑制作用があること、カエルで生長ホルモン放出活性があることが知られているPACAPに甲状腺刺激ホルモン放出活性があることがわかった。これらの結果から甲状腺刺激ホルモンは多種類のペプチドにより調節されており、はじめに哺乳動物で名付けられたペプチドの名称が必ずしもあてはまらないことが明らかになった。　一方我々はイモリ視床下部のプロラクチン受容体発現部位の解析中に、下垂体後葉ホルモン含有神経にプロラクチン受容体が発現していることを発見した。現在プロラクチンの後葉ホルモン放出調節の可能性を検討している。
脳内ペプチドの下垂体および正中隆起部レベルにおける作用

2001年

　概要を見る

研究期間の２年間で以下の成果を得た。１）我々はカエル脳よりC末端にPhe-Met-Arg-Phe-NH2の配列を有するアミノ酸１２残基からなる新しいペプチドを単離した。その抗体を作製し、免疫組織学的に局在をしらべたところ正中隆起部に結末を有することからこのペプチドが下垂体機能を調節している可能性が示唆された。このペプチドを下垂体培養系に添加し、各種下垂体ホルモンの放出をしらべたところ、成長ホルモン放出を特異的に促進することを見い出した。このペプチドは試験管内でだけでなく、動物に静脈注射しても活性を示すことも確かめた。２）哺乳類の胃で発見されたグレリン（Ghrelin)は成長ホルモン放出を促進する事が知られているが、我々はカエル胃より２種のグレリン相当物質を単離した。この物質は２８および２９アミノ酸残基からなり、成長ホルモンの他にプロラクチンの放出を促進することを明らかにした。この物質の前駆体遺伝子は胃、腸、膵臓、精巣で発現しており、一般循環によって下垂体に達して作用すると考えられる。３）カエル脳下垂体の甲状腺刺激ホルモン含有細胞にアクチビン／インヒビンのβBサブユニット免疫陽性物質の存在を認めた。そこでアクチビンBやインヒビンBが下垂体ホルモンの放出を傍分泌的に調節している可能性をさぐるためアクチビンBおよびインヒビンBの各種下垂体ホルモンの放出におよぼす影響をしらべた。アクチビンBは生殖腺刺激ホルモンの放出を促進したが、インヒビンBは単独では効果を示さず、アクチビンBによる生殖腺ホルモンの放出活性上昇を抑制することを確かめた。４）我々は海外共同研究者との研究によりカエル脳より成長ホルモン放出ホルモン（GHRH)およびPACAPの単離、同定、その前駆体遺伝子のクローニングおよび生理活性をしらべた。生理活性に関しては両者に成長ホルモン放出活性が認められた。魚類ではPACAPのみに、哺乳類ではGHRHのみに活性があることを考えると、ホルモン分子（および受容体）の進化という観点から興味ある発見である。
フェロモンおよびホルモンによる生殖行動の調節

1999年石居　進, 小口　敦子

　概要を見る

　有尾両性類の生殖行動には、化学情報伝達物質として外分泌物質（フェロモン）が重要な役割を担っている。我々は、すでに雄アカハライモリの腹腺より雌誘引フェロモン（ソデフリン）を単離・同定しているが、この物質は同属異種のシリケンイモリには効果がない。シリケンイモリは別の物質をフェロモンとして用いていると推測し、ソデフリンに相当する物質を、遺伝子及びペプチド面から解析を進めた。その結果、すでに得られているソデフリン前駆体cDNAに相当するcDNAが得られ、それにソデフリンとアミノ酸２残基がいれかわったペプチドがコードされていることがわかった。更に、シリケンイモリの腹腺からこのペプチドの単離を逆相HPLCを用いて進め、アミノ酸10残基よりなるペプチドを得た。生物活性を調べた結果、この物質（単離した物質及び合成品ともに）はシリケンイモリの雌に対して強い誘引活性を持つがアカハライモリに対しては活性を示さないことがわかり、種特異性があることが証明された。同物質はＮ末端のアミノ酸３残基の略号SILとソデフリンの末尾efrinとあわせてシリフリン（silefrin）と名付けられた。更に、ソデフリン及びシリフリン前駆体cDNAをプローブとして用いて前駆体mRNAの発現及び２つのフェロモンの放射免疫測定法による腹腺含量の測定を可能にし、これらを指標にこの２つのフェロモンの合成がいかなるホルモンによって促進されるかを調べた。その結果、プロラクチンと雄性ホルモンの組み合わせで顕著に両フェロモンの合成が促進されることを明らかにした。尚、フェロモンの放出はアルギニンヴァソトシンにより促されること、このホルモンはＶ１受容体を介して作用することも明らかにした。
脳－脳下垂体系研究の新展開

1998年石居　進, 山内　兄人, 川内　浩司, 平野　哲也, 新井　康允, A.　Fasolo, F.　Gracia-Navarro, A.　Polzonetti, H.　Vaudry, A.　Gorbman, G.　Grau

　概要を見る

　上記研究課題に関する国際会議を実現させるために、代表者および分担者は主たる関連国際誌上で公表されている研究内容、レベルの把握を心掛け、また国内分担者は研究期間中に開催された関連がありそうな国内外の約40回に渡る会議・シンポジウムに参加し情報の収集につとめるとともに国外研究者との交流をはかった。　特に1997年に早稲田大学で行った脊椎動物のホルモンおよび神経ペプチドの分子祖先に関する会議では、多数の関連研究者の出席のもと活発な意見の交換を行った。一連の活動を通じて、脳および下垂体のほかに嗅覚系を会議のテーマとして加えることが必要であるという結論に達した。その理由としては、生体内でホルモンのほかにフェロモンが化学情報伝達に関与していること、特にフェロモン受容器官としての鋤鼻器官がヒトに存在するかという問題に注目が集まっており、フェロモン関連分野の研究の発展は目覚ましいものがあること、脳のある種の神経ペプチド産生細胞は嗅上皮由来であり、下垂体と嗅覚系は発生学上近縁の細胞群より分化すること、などが挙げられる。そこで国内の鋤鼻研究会の主たるメンバーとも意見の交換を行い、脳と下垂体に加えて嗅覚系を入れて「国際会議　脳・鼻・下垂体」を2000年に早稲田大学で開催することに決定した。
生殖における嗅覚系の役割

1997年石居　進, 高橋　則行

　概要を見る

イモリより単離した雌誘引フェロモン（ソデフリン）は、鼻腔をふさいだ動物や嗅球と嗅上皮間を遮断した動物には無効であることを確認したうえで、このフェロモンが嗅上皮のどの部分で感知するかを調べた。その結果、フェロモンによって鼻腔側室部の嗅上皮でもっとも強い嗅電図記録が得られることがわかった。この部分は有尾目では原始的鋤鼻器官と見做されている部分であり、哺乳類では鋤鼻器官がフェロモンの受容部位である。イモリでこの器官の上皮を形態学的に調べたところ、哺乳類鋤鼻器官の上皮細胞と同様に微絨毛のみをそなえていた。この部分にDiIを注入し、中枢のどこに神経が投射しているかを調べた結果、主として副嗅球に投射していることがわかった。一方、ソデフリンの一次構造から推定される塩基配列をもとに、ソデフリンのcDNAノクローニングを試みたところ、1360bpからなるcDNAが得られ、そのうち570塩基がソデフリン前駆体をコードしていることがわかり、ソデフリン分子は前駆体のC末端に存在することがわかった。研究成果の発表：Kikuyama, S., Toyoda, F., Iwata, T., Umezawa, K., Takahashi, N., Matsukawa, H. and Miura, S. 1998: Sodefrin, A novel peptide pheromone in the newt cloacal gland. Trends in Comparative Endocrinology and Neurobiology. in press, NY Academy of SciencesKikuyama, S., Toyoda, F., Yamamoto, K., Tanaka, S. and Hayashi, H.1997: Female-attracting pheromone in newt cloacal glands. Brain Res. Bull. 44:415-422 ElsevierYamamoto, K., Takahashi, N., Shioda, A., Kobayashi, T., Nakai, T., MiuraS., Kouki, T. and Kikuyama, S.1997: Recombinant newt growth hormone and its application to radioimmunoassay. Advances in Comparative Endocrinology. pp. 921-925 Monduzzi EditoreKikuyama, S.1997: Newt sex pheromone: Hormonal control of its sectretion. Advances in Comparative Endocrinology.pp. 349-354 Monduzzi EditoreIwata, T., Takahashi, N., Miura, S., Toyoda, F., Hayashi, H. and Kikuyama, S.1997: Molecular cloning of sodefrin-like peptide cDNA of the sword-tailed newt. Advances in Comparative Endocrinology. pp. 1747-1750 Monduzzi EditoreKato, T., Matsukawa, H., Shioda, A., Miura, S., Yamamoto, K., Fujita, T. and Kikuyama, S.1997: Cloning and expression of newt prolactin receptor. Advances in Comparative Endocrinology.pp. 979-983 Monduzzi EditoreToyoda, F., Yamamoto, K., Tanaka, S. and Kikuyama, S1997: Possible involvement of arginine vasotocin in reproductive events in the male newt, Cynops pyrrhogaster. Advances in Comparative Endocrinology. pp. 365-369 Monduzzi Editore
有尾両生類の性フェロモンに関する研究

1995年石居　進, 小室　輝昌

　概要を見る

性的に成熟した雄のアカハライモリを飼育している水に，性的に成熟した雌イモリに限ってひきつけられることがわかった。腹部肛門腺を切除された雄イモリの飼育水には雌誘引活性がみられない。そこで，活性物質は肛門腺でつくられるか，そこを経由して水中に放出されると考え，肛門腺より活性物質の単離を試みた。活性物質は，ゲル濾過カラムクロマトグラフィーやプロナーゼ消化などの結果から，分子量の比較的小さいペプチドであることが，予想され，最終的には逆相カラムクロマトグラフィーによって単離することができた。アミノ酸組成や配列の解析，分子量の測定などから，NおよびC末端ともに修飾されていない，アミノ酸10残基よりなるペプチドであることが判明した。これまでに知られているどのペプチドにも該当しないことから，これをソデフリンと命名した。ソデフリンは両生類で同定されたはじめてのフェロモンであり，脊髄動物ではじめてのペプチドフェロモンである。化学合成したソデフリンは生体より得られたものと同様の活性を有すること，ソデフリン抗体を用いてソデフリンの存在部位をしらべたところ肛門腺上皮細胞中の分泌果粒中に局在していること，などもわかった。雌イモリの鼻と脳との間の連絡を外科的に断ったりするとソデフリンの効果がみられない。従ってソデフリンは鼻器官を介して作用するものと考えられた。更にソデフリンを他種のイモリ（シリケンイモリ）に作用させても無効であり，シリケンイモリの肛門腺水抽出物はシリケンイモリ雌のみをひきつけ，アカハライモリ雌をひきつけない。このことからソデフリンは種特異性を有し，おそらく同属・異種のシリケンイモリにはソデフリンとは異なる雌誘引物質がその肛門腺に存在していることが示唆された。

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