Details of a Researcher

KUBOTA, Yuji

写真a

Affiliation	Faculty of Science and Engineering, Waseda Research Institute for Science and Engineering
Job title	Junior Researcher(Assistant Professor)

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Concurrent Post 【 display / non-display 】

Faculty of Science and Engineering School of Creative Science and Engineering

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Education 【 display / non-display 】

　

　

　

Waseda University Graduate School of Creative Science and Engineering

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Research Experience 【 display / non-display 】

2020.09

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Now

Waseda University Research Institute for Science and Engineering Assistant Professor
1996.04

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Now

興研株式会社

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Professional Memberships 【 display / non-display 】

　

　

　

The American Industrial Hygiene Association
　

　

　

The Visualization Society of Japan
　

　

　

The Japan Society of Mechanical Engineers
　

　

　

The Society of Heating, Air-Conditioning and Sanitary Engineers of Japan
　

　

　

Japan Air Cleaning Association

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Research Areas 【 display / non-display 】

Safety engineering
Fluid engineering
Hygiene and public health (laboratory)

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Research Interests 【 display / non-display 】

industrial ventilation
occupational Hygiene

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Papers 【 display / non-display 】

Assessment of capacity to capture DNA aerosols by clean filters for molecular biology experiments

Yuki Morono, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

Microbes and Environments 33 ( 2 ) 222 - 226 2018

　View Summary

Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.

DOI PubMed
Development of a bench-top extra-cleanroom for DNA amplification

Hirokazu Takahashi, Takahiro Satoh, Hiroko Kanahara, Yuji Kubota, Tamaki Hirose, Hiroyuki Yamazaki, Kimiko Yamamoto, Yoshiko Okamura, Taketo Suzuki, Toshiro Kobori

BioTechniques 61 ( 1 ) 42 - 46 2016.07

　View Summary

Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

DOI PubMed
The Effects of Cross Drafts Caused by Walking on Leak Concentration and Exposure Concentration of the Indoor Circulation Capture Hood

37 ( 3 ) 102 - 113 2016.05

CiNii
屋内排気型外付け式フード側面に沿って人が移動した時に必要となる排風量について

金丸直子, 久保田裕仁, 村田克

作業環境 37 ( 2 ) 42 - 46 2016.03

CiNii
屋内排気型外付け式フードの換気効果に関する基礎的研究

金丸直子, 久保田裕仁, 村田克

作業環境 36 ( 4 ) 38 - 41 2015.07

CiNii