Details of a Researcher

写真a

KUBOTA, Yuji

Affiliation

Faculty of Science and Engineering, Waseda Research Institute for Science and Engineering

Job title

Junior Researcher(Assistant Professor)

Concurrent Post

Faculty of Science and Engineering School of Creative Science and Engineering

Education

　

　

　

Waseda University Graduate School of Creative Science and Engineering

Research Experience

2020.09

-

Now

Waseda University Research Institute for Science and Engineering Assistant Professor
1996.04

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Now

興研株式会社

Professional Memberships

　

　

　

The American Industrial Hygiene Association
　

　

　

The Visualization Society of Japan
　

　

　

The Japan Society of Mechanical Engineers
　

　

　

The Society of Heating, Air-Conditioning and Sanitary Engineers of Japan
　

　

　

Japan Air Cleaning Association
　

　

　

Japan Occupational Hygiene Accociation
　

　

　

Japan Society for Occupational Health

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Research Areas

Safety engineering
Fluid engineering
Hygiene and public health (laboratory)

Research Interests

industrial ventilation
occupational Hygiene

Papers

Assessment of capacity to capture DNA aerosols by clean filters for molecular biology experiments

Yuki Morono, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

Microbes and Environments 33 ( 2 ) 222 - 226 2018

　View Summary

Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.

DOI PubMed
Development of a bench-top extra-cleanroom for DNA amplification

Hirokazu Takahashi, Takahiro Satoh, Hiroko Kanahara, Yuji Kubota, Tamaki Hirose, Hiroyuki Yamazaki, Kimiko Yamamoto, Yoshiko Okamura, Taketo Suzuki, Toshiro Kobori

BioTechniques 61 ( 1 ) 42 - 46 2016.07

　View Summary

Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

DOI PubMed
The Effects of Cross Drafts Caused by Walking on Leak Concentration and Exposure Concentration of the Indoor Circulation Capture Hood

37 ( 3 ) 102 - 113 2016.05

CiNii
屋内排気型外付け式フード側面に沿って人が移動した時に必要となる排風量について

金丸直子, 久保田裕仁, 村田克

作業環境 37 ( 2 ) 42 - 46 2016.03

CiNii
屋内排気型外付け式フードの換気効果に関する基礎的研究

金丸直子, 久保田裕仁, 村田克

作業環境 36 ( 4 ) 38 - 41 2015.07

CiNii
The Required Exhaust Air Volume of External Hood is determined by Leak Concentration and Exposure Concentration

36 ( 3 ) 48 - 55 2015.05

CiNii
The Effect of Flanges Attached to External Hoods of Local Exhaust Ventilation System on the Air Flow Velocity near the Hood Opening and Calculation of the Required Exhaust Air Volume

35 ( 4 ) 57 - 66 2014.07

CiNii
Verification of measurement precision by detector tube method for formaldehyde

UMEZAWA Takashi, KAMIFUKUMOTO Kiyotaka, KUBOTA Yuji

61 ( 4 ) 798 - 802 2012.07

CiNii
Effectiveness of a ventilation system for preventing exposure to formaldehyde in a pathology laboratory

Journal of working environment 32 ( 1 ) 69 - 76 2011.01

CiNii
慶應大学における解剖実習室空気環境改善対策

今西宣晶, 相磯貞和, 仲嶋一範, 久保田裕仁, 上福元清隆

解剖学雑誌 83 ( Suppl. ) 152 - 152 2008.03
溶接ヒュームコレクタの吸引速度とヒューム抑制効果に関する研究

久保田裕仁, 上福元清隆, 木村一志

作業環境 27 ( 5 ) 64 - 71 2006.09

CiNii
Some Engineering Countermeasures to Reduce Exposure to Welding Fumes and Gases Avoiding Occurrence of Blow Holes in Welded Material

Takeshi IWASAKI, Yuki FUJISHIRO, Yuji KUBOTA, Jun OJIMA, Nobuyuki SHIBATA

Industrial Health 43 ( 2 ) 351 - 357 2005

DOI CiNii
PUSH PULL型換気装置の流量比に関する研究

久保田裕仁, 木村一志, 岩崎毅

作業環境 24 ( 5 ) 66 - 72 2003.09

CiNii

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Misc

周りを囲わない清浄空間形成技術オープンクリーンテクノロジー (特集コンバーティングの仕上技術)

久保田裕仁, 横野喜則

コンバーテック 38 ( 11 ) 67 - 71 2010.11

CiNii
The formation of open clean zone and removal of electricity

16 ( 10 ) 81 - 85 2010.10

CiNii
Engineering measure by the push-pull ventilation systems in dissection laboratories

54 ( 2 ) 14 - 19 2008.02

CiNii

Awards

内閣総理大臣賞

2015.10 ものづくり日本大賞
会長賞

2015.02 日本機械工業連合会会長賞
功績賞

2011.05 日本紛体工業技術協会
Best Poster Award

2007.06 American Industrial Hygiene Association

Syllabus

Practice & International Cooperation in Environmental Study

Graduate School of Creative Science and Engineering

2022 an intensive course(spring)
Science and Engineering Laboratory 1B

School of Fundamental Science and Engineering

2022 fall semester
Science and Engineering Laboratory

School of Fundamental Science and Engineering

2022 fall semester
Science and Engineering Laboratory

School of Creative Science and Engineering

2022 fall semester
Science and Engineering Laboratory 1B

School of Creative Science and Engineering

2022 fall semester
Practice & International Cooperation in Environmental Study

School of Creative Science and Engineering

2022 an intensive course(spring)
Occupational Hygiene and Risk

School of Creative Science and Engineering

2022 spring semester
Advanced Earth and Environmental Engineering 3

School of Creative Science and Engineering

2022 fall quarter
Introduction to Earth and Environmental Engineering

School of Creative Science and Engineering

2022 spring semester
Science and Engineering Laboratory 1B

School of Advanced Science and Engineering

2022 fall semester
Science and Engineering Laboratory

School of Advanced Science and Engineering

2022 fall semester

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