KUBOTA, Yuji

写真a

Affiliation

Faculty of Science and Engineering, Waseda Research Institute for Science and Engineering

Job title

Junior Researcher(Assistant Professor)

Concurrent Post 【 display / non-display

  • Faculty of Science and Engineering   School of Creative Science and Engineering

Education 【 display / non-display

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    Waseda University   Graduate School of Creative Science and Engineering  

Research Experience 【 display / non-display

  • 2020.09
    -
    Now

    Waseda University   Research Institute for Science and Engineering   Assistant Professor

  • 1996.04
    -
    Now

    興研株式会社

Professional Memberships 【 display / non-display

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    The American Industrial Hygiene Association

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    The Visualization Society of Japan

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    The Japan Society of Mechanical Engineers

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    The Society of Heating, Air-Conditioning and Sanitary Engineers of Japan

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    Japan Air Cleaning Association

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Research Areas 【 display / non-display

  • Safety engineering

  • Fluid engineering

  • Hygiene and public health (laboratory)

Research Interests 【 display / non-display

  • industrial ventilation

  • occupational Hygiene

Papers 【 display / non-display

  • Assessment of capacity to capture DNA aerosols by clean filters for molecular biology experiments

    Yuki Morono, Tatsuhiko Hoshino, Takeshi Terada, Taketo Suzuki, Takahiro Sato, Hisashi Yuasa, Yuji Kubota, Fumio Inagaki

    Microbes and Environments   33 ( 2 ) 222 - 226  2018

     View Summary

    Experimental contamination by exogenous DNA is a major issue in molecular biological studies for data quality and its management. We herein assessed DNA aerosols for the risk of contamination and tested the capacity of clean air filters to trap and remove DNA aerosols. DNA aerosols were generated by atomizing a DNA solution and introduced into a laminar flow clean air unit. Capture and detection performed upstream and downstream of the clean air unit showed that a significant fraction (>99.96%) of introduced molecules was trapped and removed by the filter. Although DNA aerosols appear to be an avoidable source of exogenous contamination, a clearer understanding and careful experimental procedures are needed in order to perform contamination-free, high-quality molecular biology experiments.

    DOI PubMed

  • Development of a bench-top extra-cleanroom for DNA amplification

    Hirokazu Takahashi, Takahiro Satoh, Hiroko Kanahara, Yuji Kubota, Tamaki Hirose, Hiroyuki Yamazaki, Kimiko Yamamoto, Yoshiko Okamura, Taketo Suzuki, Toshiro Kobori

    BioTechniques   61 ( 1 ) 42 - 46  2016.07

     View Summary

    Prevention of airborne contamination has become an important factor in biotechnology; however, conventional laminar-airflow cabinets (LAF-cabinets) are no longer sufficient as a countermeasure against nano-sized airborne contaminants in the laboratory. Here we present a bench-top extra-cleanroom classified as ISO-1 that can prevent contamination from airborne nanoparticles. This bench-top extra-cleanroom consists of a novel clean-zone-creating system that is equipped with nanofibrous, nonwoven filters. In addition, the cleanroom is also equipped with an ionizer to prevent plasticware from collecting dust by electrostatic charge attraction. This combination of features allows the cleanroom to prevent DNA contamination derived from airborne nanoparticles. Our extra-cleanroom with ionizer could be useful in various areas of biotechnology that are easily affected by airborne contaminants.

    DOI PubMed

  • 屋内排気型外付け式フード側面に沿って人が移動した時に必要となる排風量について

    金丸 直子, 久保田 裕仁, 村田 克

    作業環境   37 ( 2 ) 42 - 46  2016.03

    CiNii

  • 屋内排気型外付け式フードの換気効果に関する基礎的研究

    金丸 直子, 久保田 裕仁, 村田 克

    作業環境   36 ( 4 ) 38 - 41  2015.07

    CiNii

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Awards 【 display / non-display

  • 内閣総理大臣賞

    2015.10   ものづくり日本大賞  

  • 会長賞

    2015.02   日本機械工業連合会会長賞  

  • 功績賞

    2011.05   日本紛体工業技術協会  

  • Best Poster Award

    2007.06   American Industrial Hygiene Association  

 

Syllabus 【 display / non-display