YAMAMOTO, Yu

写真a

Affiliation

Faculty of Education and Integrated Arts and Sciences, School of Education

Job title

Research Associate

Education 【 display / non-display

  • 2017.04
    -
    Now

    Waseda University   Graduate School of Advanced Science and Engineering   Major in Integrative Bioscience and Biomedical Engineering  

  • 2015.04
    -
    2017.03

    Waseda University   Graduate School of Advanced Science and Engineering   Major in Integrative Bioscience and Biomedical Engineering  

  • 2011.04
    -
    2015.03

    Waseda University   School of Education   Department of Biology  

Research Experience 【 display / non-display

  • 2019.04
    -
    Now

    Waseda University   Faculty of Education and Integrated Arts and Sciences   Research associate

Professional Memberships 【 display / non-display

  •  
     
     

    The American Society for Cell Biology

  •  
     
     

    The Molecular Biology Society of Japan

  •  
     
     

    The Japanese Biochemical Society

 

Research Areas 【 display / non-display

  • Cell biology

  • Molecular biology

  • Genome biology

Papers 【 display / non-display

  • Cruciform Formable Sequences within Pou5f1 Enhancer Are Indispensable for Mouse ES Cell Integrity.

    Yu Yamamoto, Osamu Miura, Takashi Ohyama

    International journal of molecular sciences   22 ( 7 )  2021.03  [International journal]

     View Summary

    DNA can adopt various structures besides the B-form. Among them, cruciform structures are formed on inverted repeat (IR) sequences. While cruciform formable IRs (CFIRs) are sometimes found in regulatory regions of transcription, their function in transcription remains elusive, especially in eukaryotes. We found a cluster of CFIRs within the mouse Pou5f1 enhancer. Here, we demonstrate that this cluster or some member(s) plays an active role in the transcriptional regulation of not only Pou5f1, but also Sox2, Nanog, Klf4 and Esrrb. To clarify in vivo function of the cluster, we performed genome editing using mouse ES cells, in which each of the CFIRs was altered to the corresponding mirror repeat sequence. The alterations reduced the level of the Pou5f1 transcript in the genome-edited cell lines, and elevated those of Sox2, Nanog, Klf4 and Esrrb. Furthermore, transcription of non-coding RNAs (ncRNAs) within the enhancer was also upregulated in the genome-edited cell lines, in a similar manner to Sox2, Nanog, Klf4 and Esrrb. These ncRNAs are hypothesized to control the expression of these four pluripotency genes. The CFIRs present in the Pou5f1 enhancer seem to be important to maintain the integrity of ES cells.

    DOI PubMed