HAIJIMA, Asahi

写真a

Affiliation

Faculty of Human Sciences, School of Human Sciences

Job title

Assistant Professor(without tenure)

Research Experience 【 display / non-display

  • 2019.04
    -
    Now

    Waseda University   Faculty of Human Sciences

  • 2011.08
    -
    2019.03

    Gunma University   Assistant Professor

  • 2008.12
    -
    2011.07

    The University of Tokyo

 

Papers 【 display / non-display

  • Neurotoxic effects of lactational exposure to perfluorooctane sulfonate on learning and memory in adult male mouse

    Abdallah Mshaty, Asahi Haijima, Yusuke Takatsuru, Ayane Ninomiya, Hiroyuki Yajima, Michifumi Kokubo, Miski Aghnia Khairinisa, Wataru Miyazaki, Izuki Amano, Noriyuki Koibuchi

    Food and Chemical Toxicology   145   111710 - 111710  2020.11  [Refereed]  [International journal]

    Authorship:Corresponding author

     View Summary

    The present study aims to examine the effect of early lactational perfluorooctane sulfonate (PFOS) exposures on learning and memory in male mice and reveal the underlying mechanisms involved. PFOS solution was orally administered to dams from the postpartum days 1-14, so that pups would be exposed through breast milk. At 8-10 weeks of age, we performed object location test (OLT), object recognition test (ORT), and pairwise visual discrimination (VD) task. We also performed in vivo microdialysis, and mRNA and protein analysis of genes responsible for hippocampal development and function. In both OLT and ORT, the performance of mice in the PFOS-exposed group was significantly lower than those in the control group. In the VD task, the PFOS-exposed group learned significantly slower than the control group. Concentrations of glutamate and gamma-aminobutyric acid in the dorsal hippocampus were significantly higher in the PFOS-exposed group than in the control group. No notable differences were shown in our mRNA and protein studies. The present study showed that lactational PFOS exposure has a profound, long-lasting neurotoxic effect in the hippocampus and consequently leads to learning and memory deficits.

    DOI PubMed

  • A mouse model of Timothy syndrome exhibits altered social competitive dominance and inhibitory neuron development

    Shin‐ichiro Horigane, Yukihiro Ozawa, Jun Zhang, Hiroe Todoroki, Pan Miao, Asahi Haijima, Yuchio Yanagawa, Shuhei Ueda, Shigeo Nakamura, Masaki Kakeyama, Sayaka Takemoto‐Kimura

    FEBS Open Bio    2020.07  [Refereed]

    DOI

  • In utero and postnatal propylthiouracil-induced mild hypothyroidism impairs maternal behavior in mice

    Miski Aghnia Khairinisa, Yusuke Takatsuru, Izuki Amano, Michifumi Kokubo, Asahi Haijima, Wataru Miyazaki, Noriyuki Koibuchi

    Frontiers in Endocrinology   9 ( MAY ) 228  2018.05  [Refereed]

     View Summary

    Thyroid hormones (THs) play crucial roles in general and brain development. Even if the hypothyroidism is mild, it may alter brain function, resulting in irreversible behavioral alterations. Although various behavioral analyses have been conducted, the effects of propylthiouracil (PTU) treatment during in utero and postnatal periods on maternal behavior have not yet been studied. The present study examined in mice whether THs insufficiency during development induce behavioral changes. Pregnant C57BL/6j mice were divided into three groups, and each group was administered different dosages of PTU (0, 5, or 50 ppm) in drinking water during in utero and postnatal periods (from gestational day 14 to postnatal day 21). First, locomotor activity and cognitive function were assessed in the offspring at 10 weeks. Next, female offspring were mated with normal mice and they and their offspring were used to assess several aspects of maternal behavior (identifying first pup, returning all pups to nest, time spent nursing, and licking pups). As expected, locomotor and cognitive functions in these mice were disrupted in a PTU dose-dependent manner. On postpartum day 2, dams who had been exposed 50 ppm PTU during in utero and postnatal periods displayed a significantly longer time identifying the first pup and returning all three pups back to the nest, less time nursing, and decreased licking behavior. The decrease in maternal behavior was significantly correlated with a decrease in cognition. These results indicate that insufficiency of THs during in utero and postnatal periods impairs maternal behavior, which may be partly induced by impaired cognitive function.

    DOI

  • Early-life stress induces cognitive disorder in middle-aged mice

    Hiroyuki Yajima, Asahi Haijima, Miski Aghnia Khairinisa, Noriaki Shimokawa, Izuki Amano, Yusuke Takatsuru

    Neurobiology of Aging   64   139 - 146  2018.04  [Refereed]

     View Summary

    Early-life stress can induce several neuropsychological disorders in adulthood. However, the underlying mechanisms inducing such disorders are still not fully understood. Furthermore, the effects of early-life stress on the changes in cognitive function with age are still not clarified. In this study, we used maternal deprivation (MD) to examine the cognitive function in middle-aged mice using a touchscreen-equipped operant chamber. In the visual-discrimination task, the aged (∼1.4 years old) control mice could accurately learn to discriminate between different visual stimuli. In contrast, the correct response rate of aged MD mice increased to ∼60% by day 10
    it was still significantly lower than that of the control mice (85%). In the hippocampus of aged MD mice, the expression level of the N-methyl-d-aspartate receptor subunit GluN1 decreased significantly as compared to that in control mice. On the other hand, no significant difference in GluN1 expression level was detected in young (2.5 months old) mice. These findings indicate that early-life stress accelerates cognitive impairment in middle-aged mice.

    DOI PubMed

  • Inhibitory neuron-specific Cre-dependent red fluorescent labeling using VGAT BAC-based transgenic mouse lines with identified transgene integration sites

    Ryosuke Kaneko, Yusuke Takatsuru, Ayako Morita, Izuki Amano, Asahi Haijima, Itaru Imayoshi, Nobuaki Tamamaki, Noriyuki Koibuchi, Masahiko Watanabe, Yuchio Yanagawa

    Journal of Comparative Neurology   526 ( 3 ) 373 - 396  2018.02  [Refereed]

     View Summary

    Inhibitory neurons are crucial for shaping and regulating the dynamics of the entire network, and disturbances in these neurons contribute to brain disorders. Despite the recent progress in genetic labeling techniques, the heterogeneity of inhibitory neurons requires the development of highly characterized tools that allow accurate, convenient, and versatile visualization of inhibitory neurons in the mouse brain. Here, we report a novel genetic technique to visualize the vast majority and/or sparse subsets of inhibitory neurons in the mouse brain without using techniques that require advanced skills. We developed several lines of Cre-dependent tdTomato reporter mice based on the vesicular GABA transporter (VGAT)-BAC, named VGAT-stop-tdTomato mice. The most useful line (line #54) was selected for further analysis based on two characteristics: the inhibitory neuron-specificity of tdTomato expression and the transgene integration site, which confers efficient breeding and fewer adverse effects resulting from transgene integration-related genomic disruption. Robust and inhibitory neuron-specific expression of tdTomato was observed in a wide range of developmental and cellular contexts. By breeding the VGAT-stop-tdTomato mouse (line #54) with a novel Cre driver mouse line, Galntl4-CreER, sparse labeling of inhibitory neurons was achieved following tamoxifen administration. Furthermore, another interesting line (line #58) was generated through the unexpected integration of the transgene into the X-chromosome and will be used to map X-chromosome inactivation of inhibitory neurons. Taken together, our studies provide new, well-characterized tools with which multiple aspects of inhibitory neurons can be studied in the mouse.

    DOI PubMed

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Misc 【 display / non-display

  • 周産期の軽度甲状腺機能低下により成体マウスで観察された学習障害

    天野 出月, 高鶴 裕介, Miski Aghnia Khairinisa, 小久保 倫文, 蓜島 旭, 鯉淵 典之

    The Kitakanto medical journal = 北関東医学   65 ( 4 ) 319 - 319  2015.11

    CiNii

  • Effect of oral administration of thyroxine on cerebellar function in congenital hypothyroid(rdw)rat

    Hideka Hayashi, Anri Azuma, Hiroyuki Mizuno, Shinosuke Masuda, Yu Lu, Ronny Lesmana, Asahi Haijima, Yusuke Takatsuru, Toshiharu Iwasaki, Noriaki Shimokawa, Motoaki Umezu, Noriyuki Koibuchi

    JOURNAL OF PHYSIOLOGICAL SCIENCES   63   S214 - S214  2013

    Research paper, summary (international conference)  

Specific Research 【 display / non-display

  • げっ歯類における視覚に基づくコミュニケーションの神経基盤の解析

    2020  

     View Summary

    我々は日常的に他者とコミュニケーションをとりながら生活している。中でも他者の顔や身体を適切に認知・識別する能力は,他者の性別や年齢,意図や情動などの情報を読み取るための基盤となる重要なものである。しかし,げっ歯類では匂いを中心としたものと考えられてきたため,げっ歯類における視覚的要因やそのメカニズムについてはほとんどわかっていない。本研究では,平原ハタネズミの視覚的な他個体認知能力の評価と脳機能の解析を行うことを目的として,タッチスクリーン型オペラント箱を用いて2視覚弁別課題を行なった。その結果,平原ハタネズミが同種他個体個体を弁別できる可能性が示唆された。

  • マウスの記憶想起におよぼす経験の影響の解析

    2019  

     View Summary

    検索誘導性忘却(RIF)は,ある事柄を思い出すことを経験すると,それに関連した記憶が抑制される現象である。経験が記憶の想起に及ぼす影響を明らかにすることは、記憶メカニズムを解明するために重要な課題である。しかし記憶の神経基盤の解析に有用なマウスにおいてRIFに関する研究はほとんど行われてこなかった。そこで本研究では,マウスにおいてRIFは雌雄共に生じるのか,また若齢期および老齢期のマウスにおいてRIFが生じるかについて,新奇物体再認記憶試験を改変した行動試験を用いて検討した。その結果,RIFはマウスにおいて雌雄ともに生じること,若齢期から老齢期まで保持されることが示唆された。

 

Syllabus 【 display / non-display

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