松永 浩子 (マツナガ ヒロコ)

写真a

所属

研究院(研究機関) ナノ・ライフ創新研究機構

職名

次席研究員(研究院講師)

学歴 【 表示 / 非表示

  • 1989年04月
    -
    1993年03月

    大阪府立大学   総合科学部   総合科学科生命科学コース  

学位 【 表示 / 非表示

  • 大阪府立大学   博士(理学)

経歴 【 表示 / 非表示

  • 2018年07月
    -
    継続中

    早稲田大学   ナノ・ライフ創新研究機構   次席研究員(研究員講師)

  • 2016年06月
    -
    2018年06月

    早稲田大学   規範科学総合研究所   招聘研究員

  • 1993年04月
    -
    2018年06月

    株式会社 日立製作所   中央研究所   研究員

  • 2001年02月
    -
    2003年02月

    アイオワ州立大学   客員研究員

  • 1994年04月
    -
    1995年03月

    大阪大学   細胞生体工学センタ   受託研究員

 

研究分野 【 表示 / 非表示

  • 分子生物学

論文 【 表示 / 非表示

  • Cortical transcriptome analysis after spinal cord injury reveals the regenerative mechanism of central nervous system in CRMP2 knock-in mice.

    Ayaka Sugeno, Wenhui Piao, Miki Yamazaki, Kiyofumi Takahashi, Koji Arikawa, Hiroko Matsunaga, Masahito Hosokawa, Daisuke Tominaga, Yoshio Goshima, Haruko Takeyama, Toshio Ohshima

    Neural regeneration research   16 ( 7 ) 1258 - 1265  2021年07月  [国際誌]

     概要を見る

    Recent studies have shown that mutation at Ser522 causes inhibition of collapsin response mediator protein 2 (CRMP2) phosphorylation and induces axon elongation and partial recovery of the lost sensorimotor function after spinal cord injury (SCI). We aimed to reveal the intracellular mechanism in axotomized neurons in the CRMP2 knock-in (CRMP2KI) mouse model by performing transcriptome analysis in mouse sensorimotor cortex using micro-dissection punching system. Prior to that, we analyzed the structural pathophysiology in axotomized or neighboring neurons after SCI and found that somatic atrophy and dendritic spine reduction in sensorimotor cortex were suppressed in CRMP2KI mice. Further analysis of the transcriptome has aided in the identification of four hemoglobin genes Hba-a1, Hba-a2, Hbb-bs, and Hbb-bt that are significantly upregulated in wild-type mice with concomitant upregulation of genes involved in the oxidative phosphorylation and ribosomal pathways after SCI. However, we observed substantial upregulation in channel activity genes and downregulation of genes regulating vesicles, synaptic function, glial cell differentiation in CRMP2KI mice. Moreover, the transcriptome profile of CRMP2KI mice has been discussed wherein energy metabolism and neuronal pathways were found to be differentially regulated. Our results showed that CRMP2KI mice displayed improved SCI pathophysiology not only via microtubule stabilization in neurons, but also possibly via the whole metabolic system in the central nervous system, response changes in glial cells, and synapses. Taken together, we reveal new insights on SCI pathophysiology and the regenerative mechanism of central nervous system by the inhibition of CRMP2 phosphorylation at Ser522. All these experiments were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee at Waseda University, Japan (2017-A027 approved on March 21, 2017; 2018-A003 approved on March 25, 2018; 2019-A026 approved on March 25, 2019).

    DOI PubMed

  • Development of an Inflammatory CD14+ Dendritic Cell Subset in Humanized Mice.

    Ryutaro Iwabuchi, Keigo Ide, Kazutaka Terahara, Ryota Wagatsuma, Rieko Iwaki, Hiroko Matsunaga, Yasuko Tsunetsugu-Yokota, Haruko Takeyama, Yoshimasa Takahashi

    Frontiers in immunology   12   643040 - 643040  2021年  [国際誌]

     概要を見る

    Humanized mouse models are attractive experimental models for analyzing the development and functions of human dendritic cells (DCs) in vivo. Although various types of DC subsets, including DC type 3 (DC3s), have been identified in humans, it remains unclear whether humanized mice can reproduce heterogeneous DC subsets. CD14, classically known as a monocyte/macrophage marker, is reported as an indicator of DC3s. We previously observed that some CD14+ myeloid cells expressed CD1c, a pan marker for bona fide conventional DC2 (cDC2s), in humanized mouse models in which human FLT3L and GM-CSF genes were transiently expressed using in vivo transfection (IVT). Here, we aimed to elucidate the identity of CD14+CD1c+ DC-like cells in humanized mouse models. We found that CD14+CD1c+ cells were phenotypically different from cDC2s; CD14+CD1c+ cells expressed CD163 but not CD5, whereas cDC2s expressed CD5 but not CD163. Furthermore, CD14+CD1c+ cells primed and polarized naïve CD4+ T cells toward IFN-γ+ Th1 cells more profoundly than cDC2s. Transcriptional analysis revealed that CD14+CD1c+ cells expressed several DC3-specific transcripts, such as CD163, S100A8, and S100A9, and were clearly segregated from cDC2s and monocytes. When lipopolysaccharide was administered to the humanized mice, the frequency of CD14+CD1c+ cells producing IL-6 and TNF-α was elevated, indicating a pro-inflammatory signature. Thus, humanized mice are able to sustain development of functional CD14+CD1c+ DCs, which are equivalent to DC3 subset observed in humans, and they could be useful for analyzing the development and function of DC3s in vivo.

    DOI PubMed

  • Effective microtissue RNA extraction coupled with Smart-seq2 for reproducible and robust spatial transcriptome analysis.

    Miki Yamazaki, Masahito Hosokawa, Koji Arikawa, Kiyofumi Takahashi, Chikako Sakanashi, Takuya Yoda, Hiroko Matsunaga, Haruko Takeyama

    Scientific reports   10 ( 1 ) 7083 - 7083  2020年04月  [査読有り]  [国際誌]

     概要を見る

    Spatial transcriptomics is useful for understanding the molecular organization of a tissue and providing insights into cellular function in a morphological context. In order to obtain reproducible results in spatial transcriptomics, we have to maintain tissue morphology and RNA molecule stability during the image acquisition and biomolecule collection processes. Here, we developed a tissue processing method for robust and reproducible RNA-seq from tissue microdissection samples. In this method, we suppressed RNA degradation in fresh-frozen tissue specimens by dehydration fixation and effectively collected a small amount of RNA molecules from microdissection samples by magnetic beads. We demonstrated the spatial transcriptome analysis of the mouse liver and brain in serial microdissection samples (100 μm in a diameter and 10 μm in thickness) produced by a microdissection punching system. Using our method, we could prevent RNA degradation at room temperature and effectively produce a sequencing library with Smart-seq2. This resulted in reproducible sequence read mapping in exon regions and the detection of more than 2000 genes compared to non-fixed samples in the RNA-seq analysis. Our method would be applied to various transcriptome analyses, providing the information for region specific gene expression in tissue specimens.

    DOI PubMed

  • Highly sensitive mutation quantification by high-dynamic-range capillary-array electrophoresis (HiDy CE).

    Takashi Anazawa, Hiroko Matsunaga, Shuhei Yamamoto, Ryoji Inaba

    Lab on a chip   20 ( 6 ) 1083 - 1091  2020年03月  [査読有り]  [国際誌]

     概要を見る

    A simple and robust ultra-small four-color-fluorescence detection system was developed by integrating its components, namely, a four-capillary array, an injection-molded-plastic four-lens array, a four-dichroic-mirror array, and a CMOS sensor, as one device. The developed system was applied to a high-dynamic-range capillary-array electrophoresis (HiDy CE) to quantify a rare EGFR mutant (MT) of exon 19 deletion in a large excess of EGFR wild type (WT). Samples with serially diluted MT and constant-concentration WT were co-amplified by competitive PCR and subjected to HiDy CE. The MT peak in each electropherogram was then compared to the WT peak. As a result, MT was quantified with high-sensitivity (LOD of 0.004% MT/WT) and four-orders-of-magnitude dynamic range (0.01-100% MT/WT) by HiDy CE. Moreover, compared with existing methods, HiDy CE achieves higher speed, higher sample throughput, and lower consumable cost per sample. It has therefore great potential to be used in clinical practice.

    DOI PubMed

  • Combinatory use of distinct single-cell RNA-seq analytical platforms reveals the heterogeneous transcriptome response.

    Yukie Kashima, Ayako Suzuki, Ying Liu, Masahito Hosokawa, Hiroko Matsunaga, Masataka Shirai, Kohji Arikawa, Sumio Sugano, Takashi Kohno, Haruko Takeyama, Katsuya Tsuchihara, Yutaka Suzuki

    Scientific reports   8 ( 1 ) 3482 - 3482  2018年02月  [査読有り]  [国際誌]

     概要を見る

    Single-cell RNA-seq is a powerful tool for revealing heterogeneity in cancer cells. However, each of the current single-cell RNA-seq platforms has inherent advantages and disadvantages. Here, we show that combining the different single-cell RNA-seq platforms can be an effective approach to obtaining complete information about expression differences and a sufficient cellular population to understand transcriptional heterogeneity in cancers. We demonstrate that it is possible to estimate missing expression information. We further demonstrate that even in the cases where precise information for an individual gene cannot be inferred, the activity of given transcriptional modules can be analyzed. Interestingly, we found that two distinct transcriptional modules, one associated with the Aurora kinase gene and the other with the DUSP gene, are aberrantly regulated in a minor population of cells and may thus contribute to the possible emergence of dormancy or eventual drug resistance within the population.

    DOI PubMed

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産業財産権 【 表示 / 非表示

  • 細胞塊微細分画デバイスおよび解析方法

    松永 浩子, 田邉 麻衣子, 有川 浩司

    特許権

    J-GLOBAL

  • 環状型一本鎖核酸、およびその調製方法と使用方法

    松永 浩子, 田邉 麻衣子

    特許権

    J-GLOBAL

  • 核酸増幅反応後の反応液の解析方法、解析装置及び核酸増幅反応後の反応液処理装置

    松永 浩子, 梶山 智晴, 太田 真理, 神原 秀記

    特許権

    J-GLOBAL

  • 細胞採取システム

    白井 正敬, 角田 弘之, 松永 浩子, 内田 憲孝

    特許権

    J-GLOBAL

  • 細胞採取システム

    特許第5487152号

    白井 正敬, 角田 弘之, 松永 浩子, 内田 憲孝

    特許権

    J-GLOBAL

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