Updated on 2024/07/19

写真a

 
KAWAMURA, Takuji
 
Affiliation
Faculty of Sport Sciences, Institute for Sport Sciences
Job title
Junior Researcher(Assistant Professor)
Degree
博士(スポーツ科学) ( 2018.03 早稲田大学 )

Research Experience

  • 2024.05
    -
    Now

    Waseda University   Faculty of Sport Sciences   Junior Researcher (Assistant Professor)

  • 2021.07
    -
    2024.03

    Hungarian University of Sports Science   Research Centre for Molecular Exercise Science   Postdoctoral Fellow

  • 2021.04
    -
    2024.03

    Waseda University   Institute for Sport Sciences   Invited Researcher

  • 2018.04
    -
    2021.03

    Waseda University   Faculty of Sport Sciences   Assistant Professor

Professional Memberships

  •  
     
     

    THE JAPANESE SOCIETY OF PHYSICAL FITNESS AND SPORTS MEDICINE

  •  
     
     

    European College of Sport Science

Research Areas

  • Sports sciences   Geroscience / Sports sciences   運動生理学、スポーツ生理学

Research Interests

  • Cardiorespiratory fitness

  • Geroscience

  • aging

  • Epigentics

Awards

  • 早稲田大学スポーツ科学学術院20周年記念式典・卒業生表彰

    2023.10  

  • Hungarian state scholarships for short-term or long-term study visits

    2021.09   Tempus Public Foundation  

  • 特に優れた業績による奨学金返還免除

    2015.06   独立行政法人日本学生支援機構  

 

Papers

  • Healthy Japanese Dietary Pattern Is Associated with Slower Biological Aging in Older Men: WASEDA'S Health Study

    Kawamura T, Higuchi M, Ito T, Kawakami R, Usui C, McGreevy K.M, Horvath S, Radak Z, Torii S, Suzuki K, Ishii K, Sakamoto S, Oka K, Muraoka I, Tanisawa K

    Frontiers in Nutrition   11  2024.05  [Refereed]

    Authorship:Lead author, Corresponding author

    DOI

    Scopus

  • Polygenic risk score, cardiorespiratory fitness, and cardiometabolic risk factors: WASEDA’S Health Study

    Tanisawa K, Tabata H, Nakamura N, Kawakami R, Usui T. Ito T, Kawamura T, Torii S, Suzuki K, Muraoka I, Sakamoto S, Higuchi H, Oka K

    Medicine & Science in Sports & Exercise (in press)    2024.04

  • Organ specificity and commonality of epigenetic aging in low- and high-running capacity rats

    Kawamura T, Kerepesi C (co-first author, Torma F, Bori Z, Lei Z, Bakonyi P, Kolonics A, Laszlo B, Higuchi M, Koch LG, Britton SL, Koltai E, Radak Z

    bioRxiv    2024.04

    Authorship:Lead author

    DOI

  • Effects of palm cooling on thermoregulatory-related and subjective indicators during exercise in a hot environment

    Iwata R, Kawamura T, Okabe F, Fujita Z

    Journal of Thermal Biology    2024.01  [Refereed]

    Authorship:Lead author

  • Long-term iron supplementation combined with vitamin B6 enhances maximal oxygen uptake and promotes skeletal muscle-specific mitochondrial biogenesis in rats

    Zhou L, Mozaffaritabar S, Kolonics A, Kawamura T, Koike A, Kéringer J, Gu Y, Radak Z, Karabanov R

    Frontiers in Nutrition   10   1335187 - 1335187  2024.01  [Refereed]

  • The effect of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged mice

    Zhou L, Mozaffaritabar S, Kawamura T, Koike A, Kolonics A, Kéringer J, Pinho R, Sun J, Shangguan R, Radak Z

    Heliyon   10 ( 2 ) e24421 - e24421  2024.01  [Refereed]

    DOI

    Scopus

    1
    Citation
    (Scopus)
  • Associations between cardiorespiratory fitness and lifestyle-related factors with DNA methylation-based ageing clocks in older men: WASEDA'S Health Study

    Kawamura T*, Radak Z, Tabata H, Akiyama H, Nakamura N, Kawakami R, Ito T, Usui C, Jokai M, Torma F, Kim HK, Miyachi M, Suzuki K, Torii S, Ishii K, Sakamoto S, Oka K, Higuchi M, Muraoka I, McGreevy K.M., Horvath S, Tanisawa K.

    Aging Cell   23 ( 1 ) e13960  2024.01  [Refereed]

    Authorship:Lead author, Corresponding author

  • DNA methylation clock DNAmFitAge shows regular exercise is associated with slower aging and systemic adaptation

    Jokai M, Torma F, McGreevy KM, Koltai E, Bori Z, Babszki G, Bakonyi P, Gombos Z, Gyorgy B, Aczel D, Toth L, Osvath P, Fridvalszky M, Teglas T, Ligeti B, Kalcsevszki R, Posa A, Kujach S, Olek R, Kawamura T, Seki Y, Suzuki K, Tanisawa K, Goto S, Boldogh I, Ba X, Szabo D, Davies KJA, Horvath S, Radak Z.

    GeroScience   45 ( 5 ) 2805 - 2817  2023.10  [Refereed]

     View Summary

    Abstract

    DNAmPhenoAge, DNAmGrimAge, and the newly developed DNAmFitAge are DNA methylation (DNAm)-based biomarkers that reflect the individual aging process. Here, we examine the relationship between physical fitness and DNAm-based biomarkers in adults aged 33–88 with a wide range of physical fitness (including athletes with long-term training history). Higher levels of VO2max (ρ = 0.2, p = 6.4E − 4, r = 0.19, p = 1.2E − 3), Jumpmax (p = 0.11, p = 5.5E − 2, r = 0.13, p = 2.8E − 2), Gripmax (ρ = 0.17, p = 3.5E − 3, r = 0.16, p = 5.6E − 3), and HDL levels (ρ = 0.18, p = 1.95E − 3, r = 0.19, p = 1.1E − 3) are associated with better verbal short-term memory. In addition, verbal short-term memory is associated with decelerated aging assessed with the new DNAm biomarker FitAgeAcceleration (ρ: − 0.18, p = 0.0017). DNAmFitAge can distinguish high-fitness individuals from low/medium-fitness individuals better than existing DNAm biomarkers and estimates a younger biological age in the high-fit males and females (1.5 and 2.0 years younger, respectively). Our research shows that regular physical exercise contributes to observable physiological and methylation differences which are beneficial to the aging process. DNAmFitAge has now emerged as a new biological marker of quality of life.

    DOI

    Scopus

    14
    Citation
    (Scopus)
  • 自転車運動時における健常若年男性の部位別の皮膚血流量に及ぼす運動強度の影響

    秋山大志, 河村拓史, 村岡功

    運動生理学雑誌 (In press)   29 ( 2 ) 45 - 52  2022.11  [Refereed]

  • Investigation of factors affecting the rate of changes in endurance exercise performance by pre-cooling with ice slurry

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Japanese Journal of Physical Fitness and Sports Medicine   71 ( 4 ) 345 - 353  2022.08  [Refereed]

    DOI

    Scopus

  • Association between alcohol dietary pattern and prevalence of dyslipidaemia: WASEDA'S Health Study

    Tanisawa K, Ito T, Kawakami R, Usui C, Sawada S.S, Kawamura T, Suzuki K, Ishii K, Muraoka I, Sakamoto S, Oka K, Higuchi M

    British Journal of Nutrition   14   1 - 31  2022.06  [Refereed]

  • Association between dietary patterns and different metabolic phenotypes in Japanese adults: WASEDA’S Health Study.

    Tanisawa K, Ito T, Kawakami R, Usui C, Kawamura T, Suzuki S, Sakamoto S, Ishii K, Muraoka I, Oka K, Higuchi M

    Frontiers in Nutrition   9   779967 - 779967  2022.01  [Refereed]  [International journal]

     View Summary

    Although many studies have reported that a posteriori dietary pattern is associated with metabolic health, there is little evidence of an association between dietary patterns and different metabolic phenotypes. The present study aimed to examine the association between major dietary patterns and different metabolic phenotypes (metabolically healthy non-obese [MHNO], metabolically unhealthy non-obese [MUNO], metabolically healthy obese [MHO], and metabolically unhealthy obese [MUO]) in middle-aged and elderly Japanese adults. This cross-sectional study enrolled 2,170 Japanese adults aged ≥40 years. The four different metabolic phenotypes were determined based on the presence of obesity, abdominal obesity, hypertension, hyperglycemia, and dyslipidemia. The major dietary patterns were determined using principal component analysis based on energy-adjusted food intake. Two dietary patterns were identified: the healthy dietary pattern, which was characterized by a high intake of vegetables, fruits, potatoes, soy products, mushrooms, seaweeds, and fish; and the alcohol dietary pattern, which was characterized by a high intake of alcoholic beverages, liver, chicken, and fish. The healthy dietary pattern was associated with the MHNO and MHO phenotypes (MUNO and MUO as reference groups, respectively), and the multivariate-adjusted odds ratios (ORs) (95% confidence intervals [CIs]) in the highest quartile of healthy dietary pattern score with the lowest quartile as the reference category were 2.10 (1.40-3.15) and 1.86 (1.06-3.25), respectively. Conversely, the alcohol dietary pattern was inversely associated with the MHNO and MHO phenotypes, while the multivariate-adjusted ORs (95% CIs) in the highest quartile of the alcohol dietary pattern score with the lowest quartile as the reference category were 0.63 (0.42-0.94) and 0.45 (0.26-0.76), respectively. There were no significant interactions between sex and healthy/alcohol dietary patterns in the prevalence of the MHNO and MHO phenotypes. In conclusion, the present study's findings suggest that major dietary patterns are associated with different metabolic phenotypes in middle-aged and elderly Japanese adults. These findings provide useful evidence for maintaining metabolic health through diet regardless of obesity status.

    DOI PubMed

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    9
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  • Hormone and oxidative stress response during recovery on normoxia after hypoxic resistance exercise

    Fujii R, Nakamura N, Kawamura T, Muraoka I

    Gazzetta Medica Italiana - Archivo per le Scienze Medica (In press)    2022  [Refereed]

  • Hypertrophy of rat skeletal muscle is associated with increased SIRT1/Akt/mTOR/S6 and suppressed Sestrin2/SIRT3/FOXO1 levels

    Gombos Z, Koltai E, Torma F, Bakonyi P, Kolonics A, Aczel D, Ditroi T, Nagy P, Kawamura T, Radak Z

    International Journal of Molecular Science   22 ( 14 ) 7588  2021.07  [Refereed]

  • Effects of sex differences on breath-hold diving performance

    Peng H, Kawamura T, Akiyama H, Chang L, Iwata R, Muraoka I

    Respiratory Physiology and Neurobiology   293   103721  2021.06  [Refereed]

  • Determinants of resting oxidative stress in middle-aged and elderly men and women: WASEDA’S Health Study

    Kawamura T, Tanisawa K, Kawakami R, Usui C, Ito T, Tabata H, Nakamura N, Kurosawa S, Choi W, Ma S, Radak Z, Sawada S.S, Suzuki K, Ishii K, Sakamoto S, Oka K, Higuchi M, Muraoka I

    Oxidative Medicine and Cellular Longevity   2021   5566880  2021.05  [Refereed]

     View Summary

    Previous studies have not investigated the determinants of resting oxidative stress, including physical fitness, as it relates to redox regulation. The present study therefore was aimed at identifying lifestyle and biological factors that determine resting oxidative stress, including objectively measured physical fitness. In 873 middle-aged and elderly men and women, age and anthropometric parameters, lifestyle-related parameters, medication and supplementation status, physical fitness, biochemical parameters, and nutritional intake status, as well as three plasma oxidative stress markers: protein carbonyl (PC), F2-isoprostane (F2-IsoP), and thiobarbituric acid reactive substances (TBARS), were surveyed and measured. The determinants of PC, F2-IsoP, and TBARS in all participants were investigated using stepwise multiple regression analysis. In PC, age (β=-0.11, P=0.002), leg extension power (β=-0.12, P=0.008), BMI (β=0.12, P=0.004), and HDL-C (β=0.08, P=0.040) were included in the regression model (adjusted R2=0.018). In the F2-IsoP, smoking status (β=0.07, P=0.060), BMI (β=0.07, P=0.054), and HbA1c (β=-0.06, P=0.089) were included in the regression model (adjusted R2=0.006). In TBARS, glucose (β=0.18, P<0.001), CRF (β=0.16, P<0.001), age (β=0.15, P<0.001), TG (β=0.11, P=0.001), antioxidant supplementation (β=0.10, P=0.002), and HbA1c (β=-0.13, P=0.004) were included in the regression model (adjusted R2=0.071). In conclusion, the present study showed that age, anthropometric index, lifestyle-related parameters, medication and supplementation status, objectively measured physical fitness, biochemical parameters, and nutritional intake status explain less than 10% of oxidative stress at rest.

    DOI

    Scopus

    6
    Citation
    (Scopus)
  • Comparison of the effect of post-exercise cooling with ice slurry ingestion between males and females

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Journal of Thermal Biology   99   102979  2021.05  [Refereed]

  • Female susceptible to fatigue fracture are resistant to muscle injury: Potential role of COL1A1 variant.

    Miyamoto-Mikami E, Kumagai H, Tanisawa K, Taga Y, Hirata K, Kikuchi N, Kamiya N, Kawakami R, Midorikawa T, Kawamura T, Kakigi R, Natsume T, Zempo H, Suzuki K, Kohmura Y, Mizuno K, Torii S, Sakamoto S, Oka K, Higuchi M, Naito H, Miyamoto N, Fuku N

    Medicine & Science in Sports & Exercise (epub ahead of print)   53 ( 9 ) 1855 - 1864  2021.03  [Refereed]

    DOI

  • Visceral fat and cardiorespiratory fitness with prevalence of pre-diabetes/diabetes mellitus among middle-aged and elderly Japanese people: the WASEDA’S Health Study

    Usui C, Kawakami R, Tanisawa K, Ito T, Tabata H, Iizuka S, Kawamura T, Midorikawa T, Sawada S.S, Torii S, Sakamoto S, Suzuki K, Ishii K, Oka K, Muraoka I, Higuchi M

    PLOS ONE   15 ( 10 ) e0241018  2020.10  [Refereed]  [International journal]

     View Summary

    The relationships between cardiorespiratory fitness (CRF) measurements not confounded by adiposity and the prevalence of pre-diabetes mellitus (pre-DM) and diabetes mellitus (DM) are not well known. Thus, we aimed to investigate the associations of visceral fat (VF) and CRF with the prevalence of pre-DM/DM among Japanese adults. The study included 970 individuals (327 women and 643 men) who were 40-87 years old and had complete health examinations, abdominal fat area, and fitness data from WASEDA'S Health Study during 2015-2018. The VF area was measured using magnetic resonance imaging. CRF was measured using a cycle ergometer and was defined as VO2peak divided by fat free mass. The pre-DM/DM was identified based on the questionnaire and fasting blood tests. The odds ratios (ORs) and 95% confidence intervals (CIs) for prevalence of pre-DM/DM were calculated. Seventy-three participants had pre-DM and 48 participants had DM. Compared to the low VF group, the high VF group had a higher prevalence of pre-DM/DM (OR: 1.87, 95% CI: 1.18-2.96), although no significant relationship was observed between CRF and pre-DM/DM prevalence (P for trend = 0.239). The sub-group analyses also revealed no significant relationship between CRF and pre-DM/DM prevalence in the low VF group (P for trend = 0.979), although CRF values were inversely related to the prevalence of pre-DM/DM in the high VF group (P for trend = 0.024). Although CRF was not independently related to the prevalence of pre-DM/DM after adjusting for adiposity, higher VF values were related to a higher prevalence of pre-DM/DM. In addition, CRF levels were inversely associated with the prevalence of pre-DM/DM only among high VF individuals.

    DOI PubMed

    Scopus

    6
    Citation
    (Scopus)
  • Differences between sexes in thermoregulatory responses and exercise time during endurance exercise in a hot environment following pre-cooling with ice slurry ingestion

    Iwata R, Kawamura T, Hosokawa Y, Chang L, Suzuki K, Muraoka I

    Journal of Thermal Biology   94   102746  2020.10  [Refereed]

  • Thermoregulatory effects of exercising in room temperature combined with a hot water bath for different time durations in a 38°C environment

    Chang L, Kawamura T, Fujii R, Muraoka I

    Advances in Exercise and Sports Physiology   26 ( 2 ) 15 - 22  2020.10  [Refereed]

  • Influence of different preceding exercises on fat metabolism during subsequent endurance exercise

    Fujii R, Nakamura N, Kawamura T, Ikemura T, Konishi M, Suzuki K, Sakamoto S, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche (In press)   179 ( 6 ) 393 - 403  2020.06  [Refereed]

     View Summary

    BACKGROUND: Preceding exercise enhances fat metabolism during subsequent endurance exercise. However, it is unknown which type of exercise most enhances fat metabolism during subsequent exercise. The aim of this study was to examine the influence of preceding resistance or endurance exercise on fat metabolism during subsequent endurance exercise and to compare the effect with that without a preceding exercise.METHODS: Nine male subjects performed the following trials: 1) endurance exercise (E trial), 2) endurance exercise preceded by resistance exercise (R+E trial), and 3) endurance exercise preceded by endurance exercise (E+E trial). There was a 20-min break after the preceding exercise. Subsequent endurance exercise was performed on a cycle ergometer at 80% of the individual ventilatory threshold. Blood and exhaled gas were analyzed respectively.RESULTS: The R+E and E+E trials showed a significantly lower respiratory exchange ratio (RER) and a higher amount of fat oxidation during endurance exercise than those in the E trial (P<0.05). Furthermore, the E+E trial showed a significantly lower RER (0.86 +/- 0.03 vs. 0.84 +/- 0.02) and a significantly higher amount of fat oxidation (0.38 +/- 0.05 g/min vs. 0.44 +/- 0.08 g/min) than those in the R+E trial.CONCLUSIONS: This study suggested that fat oxidation during subsequent endurance exercise was enhanced when a preceding exercise was performed and that endurance exercise as a preceding exercise enhanced fat oxidation more than resistance exercise did.

    DOI

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  • The effects of sex differences on delayed-onset muscle soreness by cold-water immersion following resistance exercise

    Iwata R, Chang L, Akiyama H, Kawamura T, Suzuki K, Sakamoto S, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche (In press)    2020.02  [Refereed]

  • Application of molecular hydrogen as a novel antioxidant in sports science

    Kawamura T, Higashida K, Muraoka I

    Oxidative Medicine and Cellular Longevity   2020   2328768 - 2328768  2020.01  [Refereed]  [International journal]

     View Summary

    Molecular hydrogen (H2) is a colorless, tasteless, odorless, and minimal molecule with high flammability. Although H2 has been thought to be an inert gas in living bodies for many years, an animal study reported that inhalation of H2 gas decreased oxidative stress and suppressed brain injury caused by ischemia and reperfusion injury due to its antioxidant action. Since then, the antioxidant action of H2 has attracted considerable attention and many studies have reported on its benefits. Most studies have reported the effects of H2 on diseases such as cancer, diabetes, cerebral infarction, and Alzheimer's disease. However, little is known regarding its effects on healthy subjects and exercise. Thus far, including our study, only 6 studies have explored the effect of H2 on exercise. H2 is the smallest molecule and therefore can easily penetrate the cellular membrane and rapidly diffuse into organelles. H2 is thought to be able to selectively reduce hydroxyl radicals and peroxynitrite and does not affect physiologically reactive species. H2 can be supplied to the body through multiple routes of administration, such as oral intake of H2 water and H2 bathing. Therefore, H2 may be a potential alternative strategy for conventional exogenous antioxidant interventions in sports science. The purpose of this review is to provide evidence regarding the effects of H2 intake on changes in physiological and biochemical parameters, centering on exercise-induced oxidative stress, for each intake method. Furthermore, this review highlights possible future directions in this area of research.

    DOI PubMed

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    33
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  • Hydrogen water intake may suppress liver glycogen utilization without affecting redox biomarkers during exercise in rats

    Kawamura T, Fujii R, Higashida K, Muraoka I

    Gazzetta Medica Italiana - Archivio per le Scienze Mediche   178 ( 9 ) 611 - 617  2019.09  [Refereed]

  • Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

    Kawamura T, Suzuki K, Takahashi M, Tomari M, Hara R, Gando Y, Muraoka I

    Antioxidants   7 ( 10 ) 127  2018.09  [Refereed]  [International journal]

     View Summary

    The purpose of this study was to investigate the involvement of neutrophil dynamics and function in exercise-induced muscle damage (EIMD) and delayed-onset muscle soreness (DOMS), and the effect of molecular hydrogen (H₂) intake on these parameters. Nine healthy and active young men performed H₂ and placebo bath trial in a crossover design. They carried out downhill running (-8% slope) for 30 min at a speed corresponding to 75~85% of peak oxygen uptake (VO₂peak). Subsequently, they repeated bathing for 20 min per day for one week. Degree of muscle soreness (visual analogue scale: VAS), peripheral leukocyte counts, neutrophil dynamics and function, muscle damage, and inflammation markers were measured. Plasma interleukin (IL)-6 concentration was significantly correlated with peripheral neutrophil count, VAS, and serum creatine kinase activity, respectively, after downhill running. Peripheral neutrophil count and serum myoglobin concentration were also significantly correlated. Conversely, there were no effects of H₂ bath. These results suggest that IL-6 may be involved in the mobilization of neutrophils into the peripheral blood and subsequent EIMD and DOMS after downhill running; however, it is not likely that H₂ bath is effective for the inflammatory process that was centered on neutrophils after downhill running.

    DOI PubMed

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    27
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  • Exercise-Induced Oxidative Stress and the Effects of Antioxidant Intake from a Physiological Viewpoint.

    Kawamura T, Muraoka I

    Antioxidants   7 ( 9 ) 119  2018.09  [Refereed]

    DOI PubMed

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    200
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  • Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle

    Kawamura T, Fujii R, Li X, Higashida K, Muraoka I

    Science and Sports   33 ( 3 ) 169 - 175  2018.06  [Refereed]

     View Summary

    Objectives: The purpose of this study was to examine the effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle. Methods: Male Sprague-Dawley rats (n = 24) were randomly divided into the following four groups: control (CON), low-intensity exercise (LE), high-intensity interval exercise (HE) and incremental exercise (IE). The animals in the latter three groups performed exercises in which they ran until exhaustion as follows: Animals in the LE group ran at 20 m/min (6° graded)
    the HE group ran at 40 m/min for 30 s (0° graded) with a rest interval of 60 s between each run
    and the IE group ran at 15 m/min (6° graded), initially, with the running speed gradually increased by 5 m/min, every 10 min. Immediately following the exercise, a blood sample was drawn, and followed by the gastrocnemius muscle and liver were quickly removed. These samples were analysed for lactate concentration, oxidative stress markers, and glycogen contents. Results: Plasma oxidative stress markers in the LE and HE groups demonstrated no change, while plasma protein carbonyl (PC) levels and total antioxidant capacity (TAC) in the IE group were significantly increased compared with the CON group (P &lt
    0.05). In contrast, all the oxidative stress markers, in the skeletal muscle of the three exercise groups, demonstrated no change compared with the CON group. Conclusion: Incremental exhaustive exercise elevates the plasma PC levels and TAC, but does not change skeletal muscle oxidative stress markers in rats.

    DOI

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    7
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  • Time course of decrease in skeletal muscle mitochondrial biogenesis after discontinuation of high-fat diet

    Li X, Higashida K, Kawamura T, Higuchi M

    Journal of Nutritional Science and Vitaminology   64 ( 3 ) 233 - 238  2018.01  [Refereed]  [Domestic journal]

     View Summary

    It is known that a high-fat diet induces an increase in mitochondrial biogenesis in skeletal muscle. To examine the time course of decrease in mitochondrial biogenesis in skeletal muscle after discontinuing a high-fat diet feeding, C57BL/6 mice were fed a high-fat diet for 4 wk and then switched to the control diet for another 3 or 7 d. During the high-fat diet withdrawal period, the protein content of the mitochondrial respiratory chain decreased faster than the fatty acid oxidation enzymes. The mitochondrial DNA copy number remained high for at least 1 wk after withdrawing the high-fat diet. These results suggested that after switching to the control diet following a period of high-fat diet, the increased mitochondrial biogenesis levels are maintained for a few days, and the rate of decline is divergent between the different mitochondrial components.

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    7
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  • Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle

    Li X, Higashida K, Kawamura T, Higuchi M

    Nutrients   8 ( 4 ) 203  2016.04  [Refereed]

     View Summary

    Long-term high-fat diet increases muscle mitochondrial enzyme activity and endurance performance. However, excessive calorie intake causes intra-abdominal fat accumulation and metabolic syndrome. The purpose of this study was to investigate the effect of an alternating day high-fat diet on muscle mitochondrial enzyme activities, protein content, and intra-abdominal fat mass in rats. Male Wistar rats were given a standard chow diet (CON), high-fat diet (HFD), or alternate-day high-fat diet (ALT) for 4 weeks. Rats in the ALT group were fed a high-fat diet and standard chow every other day for 4 weeks. After the dietary intervention, mitochondrial enzyme activities and protein content in skeletal muscle were measured. Although body weight did not differ among groups, the epididymal fat mass in the HFD group was higher than those of the CON and ALT groups. Citrate synthase and beta-hydroxyacyl CoA dehydrogenase activities in the plantaris muscle of rats in HFD and ALT were significantly higher than that in CON rats, whereas there was no difference between HFD and ALT groups. No significant difference was observed in muscle glycogen concentration or glucose transporter-4 protein content among the three groups. These results suggest that an alternate-day high-fat diet induces increases in mitochondrial enzyme activities and protein content in rat skeletal muscle without intra-abdominal fat accumulation.

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    21
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  • Effects of hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness

    Takuji Kawamura, Yuko Gando, Masaki Takahashi, Reira Hara, Katsuhiko Suzuki, Isao Muraoka

    Japanese Journal of Physical Fitness and Sports Medicine   65 ( 3 ) 297 - 305  2016  [Refereed]

     View Summary

    Several studies have reported that molecular hydrogen (H2) acts as a therapeutic medical gas owing to scavenging reactive oxygen species (ROS). However, little is known about effects of H2 on exercise-induced oxidative stress. The purpose of this study was to investigate the effects of weekly hydrogen bathing on exercise-induced oxidative stress and delayed-onset muscle soreness (DOMS). Nine healthy and active young men participated in this study, and each subject performed hydrogen bathing trial and placebo bathing trial in a crossover design. The subjects performed downhill running (8 % decline) at 75 % peak oxygen uptake (VO2peak) for 30 min, and each subjects conducted hydrogen or placebo bathing for 20 min, respectively, 1-6 days after downhill running. Before and after exercise, we measured visual analogue scale (VAS) and collected blood samples (Pre-and 5 min, 60 min after the end of bathing, 1day, 2days, 3days, 7days after downhill running). Blood sample analyses include creatine kinase (CK), myoglobin (Mb), malondialdehyde (MDA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), myeloperoxidase (MPO), interleukin-6 (IL-6), interleukin-17a (IL-17a) and lactate concentrations. Weekly hydrogen bathing had no effects of exercise-induced oxidative stress and muscle damage. On the other hand, hydrogen bathing significantly reduced DOMS (VAS) 1 and 2days after downhill running (p=0.033). These findings suggest that hydrogen bath after downhill exercise can be effective for reduction of DOMS.

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Presentations

  • Combined NAD(P)H and Lipofuscin FLIM revealed the development of metabolic syndrome in the liver of epigenetically altered rats.

    Koloncs A, Kawamura T, Szipőcs R, Radak Z

    SPIE Photonics West technical conferences 

    Presentation date: 2024.01

  • ポリジェニックリスクスコアと心血管代謝リスクの関連は全身持久力により 修飾されるか?

    谷澤薫平, 田端宏樹, 中村宣博, 川上諒子, 薄井澄誉子, 伊藤智子, 河村拓史, 鳥居俊, 鈴木克彦, 村岡功, 坂本静男, 樋口満, 岡浩一朗

    第2回スポーツ遺伝学研究会 

    Presentation date: 2023.08

  • Accelerated epigenetic changes may contribute to the development of hepatic insulin resistance revealed by NADH FLIM

    Koloncs A, Kawamura T, Szipőcs R, Radak Z

    15th MiPconference: Bioenergetics 

    Presentation date: 2023.07

  • 暑熱環境下での運動後におけるアイススラリー 摂取が身体冷却に及ぼす影響の性差

    岩田理沙, 河村拓史, 細川由梨, 張黎栗, 鈴木克彦, 村岡功

    第76回日本体力医学会大会 

    Presentation date: 2021.09

  • Effects of sex differences on exercise capacity and thermoregulation to pre-cooling with ice slurry ingestion in a hot environment.

    Iwata R, Chang L, Kawamura T, Suzuki K, Muraoka I

    European college of sports science 25th annual congress 

    Presentation date: 2020.09

    Event date:
    2020.09
     
     
  • 日本人中高齢者における全身持久力および内臓脂肪と糖尿病有病率の関係:WASEDA’S Health Study

    薄井 澄誉子, 川上 諒子, 谷澤 薫平, 田端 宏樹, 伊藤 智子, 飯塚 哲司, 河村 拓史, 緑川 泰史, 澤田 亨, 鳥居 俊, 坂本 静男, 鈴木 克彦, 岡 浩一朗, 樋口 満

    第74回日本体力医学会 

    Presentation date: 2019.09

  • 常温環境下での運動トレーニングと温水浴の組み合わせが体温調節機能と運動パフォーマンスに及ぼす影響

    張 黎栗, 河村 拓史, 藤井 亮輔, 村岡 功

    第74回日本体力医学会 

    Presentation date: 2019.09

  • 水素水摂取はラットの運動時におけるレドックスバイオマーカーに影響を与えずに肝グリコーゲン利用を抑制する

    河村 拓史, 藤井 亮輔, 東田 一彦, 村岡 功

    第73回日本体力医学会 

    Presentation date: 2018.09

  • The WASEDA'S Health Study focusing on redox inter-individual variability during exercise in elderly

    Takuji Kawamura

    MIRAI Ageing Workshop 

    Presentation date: 2018.06

  • Hydrogen water suppresses skeletal muscle and liver glycogenolysis during exercise while does not affect oxidative stress, blood energy substrate and endurance performance in rats

    Takuji Kawamura, Ryosuke Fujii, Kazuhiko Higashida, Isao Muraoka

    European College of Sports Science 22nd annual congress 

    Presentation date: 2017.07

  • Effects of hydrogen water on exercise-induced oxidative stress, energy metabolism and endurance performance in rats

    Takuji Kawamura, Ryosuke Fujii, Kazuhiko Higashida, Isao Muraoka

    international symposium of SGU health and sports science model 

    Presentation date: 2017.03

  • Effects of different exhaustive exercise on oxidative stress markers in rat plasma ans skeletal muscle

    Takuji Kawamura, Ryosuke Fujii, Xi li, Kazuhiko Higashida, Isao Muraoka

    The Journal of Physical Fitness and Sports Medicine 

    Presentation date: 2016.09

  • Effects of different pre-exercises on lipolysis

    Ryosuke Fujii, Nobuhiro Nakamura, Takuji Kawamura, Tsukasa Ikemura, Masayuki Konishi, Katsuhiko Suzuki, Shizuo Sakamoto, Isao Muraoka

    Presentation date: 2016.09

  • 運動時の酸化ストレスおよび遅発性筋痛に及ぼす水素の影響

    河村 拓史, 丸藤 祐子, 高橋 将記, 原 怜来, 鈴木 克彦, 村岡 功

    第70回日本体力医学会 

    Presentation date: 2015.09

  • 水素摂取が一過性持久運動時の酸化ストレスに及ぼす影響

    河村 拓史, 藤井 亮輔, 東田 一彦, 村岡 功

    第2回アクティブエイジング研究所 研究報告会 

    Presentation date: 2015.03

  • Alternate-day high-fat diet feeding induces insulin resistance despite no abdominal fat accumulation and mitochondrial dysfunction

    Kazuhiko Higashida, Takuji Kawamura, Mitsuru Higuchi  [Invited]

    Medicine and Science in Sports and Exercise 

    Presentation date: 2014.05

▼display all

Research Projects

  • 運動により分泌されるエクソソームの機能解析と生理的役割の解明

    日本学術振興会  科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))

    Project Year :

    2020.10
    -
    2024.03
     

    鈴木 克彦, 川西 範明, 河村 拓史

     View Summary

    2年目には、マウスから採取した骨格筋組織を用いたex vivo実験を行い、培養骨格筋から分泌される2年目には、マウスから採取した骨格筋組織を用いたex vivo実験を行い、培養骨格筋から分泌されるエクソソームを対象としたRNAの網羅的な発現解析法を確立した。加齢などにより異常を引き起こした骨格筋ではエクソソーム分泌動態も異常をきたしていることが想定され、分泌異常が様々な病態を引き起こしている可能性があることから、加齢モデルマウスの骨格筋から分泌されるエクソソームを対象として質的な変動解析を行った。4カ月齢の若齢マウスおよび27カ月齢の高齢マウスから採取した培養骨格筋由来のエクソソームのmicroRNAおよびmRNAの差次的発現解析を実施した結果、加齢により発現が増減する特異的なmicroRNA25種類およびmRNA370種類を見出した(第77回日本体力医学会発表発表予定、論文準備中)。KEGG パスウェイ解析により、 加齢により増加するmicroRNAはFatty acid metabolismなどの代謝経路に影響を及ぼすことが示された。
    また、これまでに我々は電気刺激による筋収縮負荷がマウス血清エクソソームのmicroRNAの発現プロファイルを変化させることを明らかにしてきた(第76回日本体力医学会発表、論文投稿中)。2年目には、筋収縮刺激により分泌される血清エクソソームが脳神経細胞の機能に影響を及ぼす影響について検証した。マウス胎児海馬由来の初代神経細胞に血清エクソソームを添加することによる mRNA の差次的発現解析を実施した結果、筋収縮負荷のマウス血清エクソソームは 132 種類の mRNA の発現を変化させることを見出した(論文準備中)。また、KEGG パスウェイ解析により、神経細胞の増殖・分化に関連する Hippo シグナル伝達経路に影響を及ぼすことが示された。

  • Effect of long-term exercise training on epigenetic clock

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

    Project Year :

    2020.04
    -
    2023.03
     

  • Whether the molecular hydrogen improve endurance performance by decreasing oxidative stress?

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

    Project Year :

    2015.04
    -
    2018.03
     

    Muraoka Isao, Higashida Kazuhiko, Ikemura Tsukasa, Kawamura Takuji, Fujii Ryosuke

     View Summary

    The purpose of this research was to obtain basic knowledge for application of molecular hydrogen (H2), which is attracting attention as a new antioxidant agent in recent years, in the health and sports science field. As a result of this study, there was no effect of H2 intake on exercise-induced oxidative stress, and endurance performance. However, it was shown that H2 significantly suppresses the decrease in liver glycogen content during endurance exercise.
    These evidences suggest that intake of H2 suppresses the utilization of liver glycogen during exercise via physiological action other than antioxidation. Further research is needed on the mechanism that H2 suppressed the utilization of liver glycogen.

Misc

  • Combined NAD(P)H and lipofuscin FLIM revealed the development of metabolic syndrome in the liver of epigenetically altered rats

    Kolonics A, Kawamura T, K, Szipocs R, Radak Z

    Single Molecule Spectroscopy and Superresolution Imaging XVII     1284909  2024.03  [Refereed]

    Research paper, summary (international conference)  

  • Cardiorespiratory fitness and visceral fat on prevalent diabetes in Japanese adults: WASEDA'S Health Study

    Usui Chiyoko, Kawakami Ryoko, Tanisawa Kumpei, Tabata Hiroki, Ito Tomoko, Iizuka Satoshi, Kawamura Takuji, Midorikawa Taishi, Sawada Susumu, Torii Suguru, Sakamoto Shizuo, Suzuki Katsuhiko, Ishii Kaori, Oka Koichiro, Higuchi Mitsuru

    The Journal of Physical Fitness and Sports Medicine   8 ( 6 ) 364 - 364  2019.11

    Research paper, summary (national, other academic conference)  

  • Alternate-day High-fat Diet Feeding Induces Insulin resistance Despite No Abdominal Fat Accumulation And Mitochondrial Dysfunction

    Kazuhiko Higashida, Takuji Kawamura, Isao Muraoka, Mitsuru Higuchi

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   47 ( 5 ) 411 - 412  2015.05

    Research paper, summary (international conference)  

    DOI

 

Teaching Experience

  • 運動生理学演習

    城西大学薬学部  

  • 運動生理学

    城西大学現代政策学部  

  • スポーツ教養演習Ⅱ

    早稲田大学スポーツ科学学術院  

  • スポーツ教養演習I

    早稲田大学スポーツ科学学術院  

 

Internal Special Research Projects

  • エピジェネティックな老化に対する全身持久力の影響

    2020  

     View Summary

     本研究の目的は、全身持久力の違いがエピジェネティックな老化に及ぼす影響を明らかにすることであった。本研究課題は、科学研究費助成事業の若手研究による「エピジェネティック時計に及ぼす長期的な運動トレーニングの効果」と連動して実施した。2020年度は、65歳以上の高齢男性の血液試料を用いてDNAの抽出を行った。また、DNAサンプルを海外に輸送・解析するための手続きを行い、本学の倫理委員会の承認を得た。新型コロナウイルス感染症拡大の影響により、当初予定していた海外におけるエピジェネティック時計の解析が実施できなかったものの、今後の感染状況の動向を見極めながら、DNAサンプルの解析を進める予定である。

  • 運動に対するレドックス応答の個別性とその決定因子の検討

    2019  

     View Summary

     本研究の目的は、運動に対するレドックス応答の個人間変動とその関連因子を明らかにすることであった。本研究課題は、2019年度特定課題研究助成費(若手・アーリーキャリア支援)による「運動に対するレドックス応答の個人間変動性の定量化」と連動して実施した。若年男性15名に対し、トレッドミルを用いた最大酸素摂取量の測定を実施し、運動前後で採血および採尿を行った。今後も研究を継続し、被験者数を増やしたうえで、運動前後におけるレドックスバイオマーカーの測定を行い、これらの個人間変動を定量化する予定である。また、個人間変動を決定しうる生理的因子についても併せて検討する。

  • 中高齢者の安静時におけるレドックス個別性とその決定因子の検討

    2019  

     View Summary

     本研究の目的は、中高齢男性を対象に、安静時における生体内の酸化‐還元(レドックス)個別性を定量化するとともに、その決定因子を明らかにすることであった。これまでに中高齢男性(40歳以上)477名の血中F2-イソプロスタン、チオバルビツール酸反応性物質およびカルボニル化タンパク質の測定を行い、併せて、身体フィットネス、動脈硬化度、腹部および下腿周径囲、内臓脂肪量および骨密度を測定した。現在、他の研究費を併せて使用することにより、女性を含めて720人分の血液サンプルの測定を終えることができた。今後は、1000検体の測定を終えた時点で、他の生理学的指標との関連性を検討する予定である。

  • 運動に対するレドックス応答の個人間変動性の定量化

    2019  

     View Summary

     本研究の目的は、運動に対するレドックス応答の個人間変動を定量化することであった。若年男性15名に対し、トレッドミルによる漸増運動負荷試験を実施し、その前後で血液および尿サンプルを得た。現在、得られた生体試料を用いて血漿のカルボニル化タンパク質濃度、赤血球のグルタチオンレベルおよび尿中のF2-イソプロスタン濃度の測定を行っているところである。今後、全てのレドックスバイオマーカーの測定を終えた段階で、運動に対するレドックス応答の個人間変動を定量化する。また、各レドックスバイオマーカーの運動前後での変化率と身体組成や呼気ガスパラメータなどの生理的因子との関連性を検討する予定である。

  • 運動時の肝および筋グリコーゲン利用に及ぼす水素水摂取の影響

    2018  

     View Summary

    The purpose of this study was to examine the effect of chronic molecularhydrogen (H2) intake on liver and skeletal muscle glycogenutilization during exercise in rats. Male SD rats were randomly divided intofour groups: control (C), H2 water (H2), control-exercise (C-Ex), H2water-exercise (H2-Ex). Each group was given mineral water or H2water ad libitum for 2 weeks. Then theexercise groups (C-Ex and H2-Ex) were performed treadmill running at18 m/min (6° graded) for 60 min. Blood, skeletal muscle and liver sampleswere analysed for tissue glycogen contents and blood energy substrats (i.e., glucose,free fatty acid, lactate). Two-way ANOVA revealed that H2 intake decreasedfree fatty acid (p &lt; 0.001) andincreased lactate concentration (p =0.004). In comparison of the change from the rest in C-Ex and H2-Exgroups, blood free fatty acid and liver glycogen content were significantly lowerin H2-Ex group than in the C-Ex group (p =0.001, p = 0.035, respectively). On the other hand, bloodlactate concentration was significantly higher in the H2-Ex group than inthe C-Ex group. These results suggest that chronic H2 water intake mayincrease liver glycogen utilization without changing muscle glycogenutilization during exercise.