2022/06/26 更新

写真a

サムエル アショック ザチャリア
サムエル アショック ザチャリア
所属
研究院(研究機関) ナノ・ライフ創新研究機構
職名
次席研究員(研究院講師)

学歴

  • 2006年01月
    -
    2012年07月

    Indian Institute of Science  

経歴

  • 2017年11月
    -
    継続中

    Waseda University

  • 2014年04月
    -
    2017年10月

    National Chiao Tung University

  • 2013年01月
    -
    2014年03月

    学習院大学

所属学協会

  • 2019年
    -
    2020年

    International Marine Biotechnology Association

  • 2019年
    -
     

    Chemical Society of Japan

  • 2014年
    -
    2015年

    Chemical Society of Japan

  • 2013年10月
    -
    2014年10月

    Society for Applied Spectroscopy (SAS)

 

研究分野

  • 基礎解析学

  • 分子生物学   Raman Imaging, Multivariate analysis

研究キーワード

  • Developing analytical methods for general applications of Raman spectroscopy

  • Raman imaging of biological systems

  • Mid IR imaging

  • Fluorescence imaging

  • Raman instrumentation

  • PCA, MCR, SVD, Cluster analysis, 2D correlation analysis

  • Single cell imaging

  • Polymers

  • Biophotonics (Spectral Imaging)

  • Multivariate statistical analyses of spectral data

  • Raman spectroscopy

▼全件表示

論文

  • Raman Microspectroscopy Imaging Analysis of Extracellular Vesicles Biogenesis by Filamentous Fungus Penicilium chrysogenum

    Ashok Zachariah Samuel, Shumpei Horii, Takuji Nakashima, Naoko Shibata, Masahiro Ando, Haruko Takeyama

    Advanced Biology     2101322 - 2101322  2022年03月  [査読有り]

    担当区分:筆頭著者

    DOI DOI2

  • Deconstruction of Obscure Features in SVD-Decomposed Raman Images from P. chrysogenum Reveals Complex Mixing of Spectra from Five Cellular Constituents

    Ashok Zachariah Samuel, Shumpei Horii, Masahiro Ando, Haruko Takeyama

    Analytical Chemistry   93 ( 35 ) 12139 - 12146  2021年09月

    担当区分:筆頭著者, 責任著者

    DOI

  • Molecular profiling of lipid droplets inside HuH7 cells with Raman micro-spectroscopy

    Ashok Zachariah Samuel, Rimi Miyaoka, Masahiro Ando, Anne Gaebler, Christoph Thiele, Haruko Takeyama

    Communications Biology   3 ( 1 ) 372 - 372  2020年12月  [査読有り]  [国際誌]

    担当区分:筆頭著者

     概要を見る

    Raman imaging has become an attractive technology in molecular biology because of its ability to detect multiple molecular components simultaneously without labeling. Two major limitations in accurately accounting for spectral features, viz., background removal and spectral unmixing, have been overcome by employing a modified and effective routine in multivariate curve resolution (MCR). With our improved strategy, we have spectrally isolated seven structurally specific biomolecules without any post-acquisition spectral treatments. Consequently, the isolated intensity profiles reflected concentrations of corresponding biomolecules with high statistical accuracy. Our study reveals the changes in the molecular composition of lipid droplets (LDs) inside HuH7 cells and its relation to the physiological state of the cell. Further, we show that the accurate separation of spectral components permits analysis of structural modification of molecules after cellular uptake. A detailed discussion is presented to highlight the potential of Raman spectroscopy with MCR in semi-quantitative molecular profiling of living cells.

    DOI PubMed

  • Detection of Penicillin G Produced by Penicillium chrysogenum KF 425 in Vivo with Raman Microspectroscopy and Multivariate Curve Resolution-Alternating Least Squares Methods

    Shumpei Horii, Masahiro Ando, Ashok Z. Samuel, Akira Take, Takuji Nakashima, Atsuko Matsumoto, Yōko Takahashi, Haruko Takeyama

    J. Nat. Prod.   Accepted  2020年03月  [査読有り]

     概要を見る

    <title>ABSTRACT</title>Raman microspectroscopy is a minimally invasive technique that can identify molecular structure without labeling. In this study, we demonstrate in vivo detection of the bioactive compound penicillin G inside <italic>Penicillium chrysogenum</italic> KF425 fungus cells. Highly overlapped spectroscopic signatures acquired using Raman microspectroscopic imaging are analyzed using a multivariate curve resolution-alternating least squares (MCR-ALS) method to extract the pure spectra of individual molecular constituents. In addition to detecting multiple constituents such as proteins and lipids, we observe the subcellular localization of penicillin G like granule particle inside the fungus body. To date, there have been no reports of direct visualization of intracellular localization of penicillin G. The methodology we present in this article is expected to be applied as a screening tool for the production of bioactive compounds by microorganisms.

    DOI

  • Direct estimation of polymer crystallinity with Raman spectroscopy using ratio of scattering cross-sections estimated from variable temperature measurements

    Ashok Zachariah Samuel

    Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy   224   117431 - 117431  2020年01月  [査読有り]

    担当区分:筆頭著者, 責任著者

    DOI

  • Estimating Percent Crystallinity of Polyethylene as a Function of Temperature by Raman Spectroscopy Multivariate Curve Resolution by Alternating Least Squares

    Ashok Zachariah Samuel, Bo-Han Lai, Shih-Ting Lan, Masahiro Ando, Chien-Lung Wang, Hiro-o Hamaguchi

    Analytical Chemistry   89 ( 5 ) 3043 - 3050  2017年03月  [査読有り]

    担当区分:筆頭著者

    DOI

  • Rapid microstructure characterization of polymer thin films with 2D-array multifocus Raman microspectroscopy

    Ashok Zachariah Samuel, Sohshi Yabumoto, Kenichi Kawamura, Koichi Iwata

    The Analyst   140 ( 6 ) 1847 - 1851  2015年  [査読有り]

    担当区分:筆頭著者

     概要を見る

    <p>Multifocus Raman imaging is one of the fast-imaging alternatives to the conventional single point mapping technique.</p>

    DOI

  • Energy funneling and macromolecular conformational dynamics: a 2D Raman correlation study of PEG melting

    Ashok Zachariah Samuel, Siva Umapathy

    Polymer Journal   46 ( 6 ) 330 - 336  2014年06月  [査読有り]

    担当区分:筆頭著者

    DOI

  • Conformational Reorganization of Hyperbranched and Linear Polymers and Functionalized Porous Polymer Films

    Ashok Zachariah Samuel

       2014年  [査読有り]

    担当区分:筆頭著者

  • Janus Hybramers: Self-Adapting Amphiphilic Hyperbranched Polymers

    Ashok Zachariah Samuel, S. Ramakrishnan

    Macromolecules   45 ( 5 ) 2348 - 2358  2012年03月  [査読有り]

    担当区分:筆頭著者

    DOI

  • Functionalized and Postfunctionalizable Porous Polymeric Films through Evaporation-Induced Phase Separation Using Mixed Solvents

    Ashok Zachariah Samuel, S. Umapathy, S. Ramakrishnan

    ACS Applied Materials & Interfaces   3 ( 9 ) 3293 - 3299  2011年09月  [査読有り]

    担当区分:筆頭著者

    DOI

  • On Selecting a Suitable Spectral Matching Method for Automated Analytical Applications of Raman Spectroscopy.

    Ashok Zachariah Samuel, Ryo Mukojima, Shumpei Horii, Masahiro Ando, Soshi Egashira, Takuji Nakashima, Masato Iwatsuki, Haruko Takeyama

    ACS omega   6 ( 3 ) 2060 - 2065  2021年01月  [国際誌]

    担当区分:筆頭著者, 責任著者

     概要を見る

    Raman spectra are molecular structure-specific and hence are employed in applications requiring chemical identification. The advent of efficient handheld and smartphone-based Raman instruments is promoting widespread applications of the technique, which often involve less trained end users. Software modules that enable spectral library searches based on spectral pattern matching is an essential part of such applications. The Raman spectrum recorded by end users will naturally have varying levels of signal to noise (SN), baseline fluctuations, etc., depending on the sample environment. Further, in biological, forensic, food, pharmaceuticals, etc., fields where a vast amount of Raman spectral data is generated, careful removal of background is often impossible. In other words, a 100% match between the library spectrum and user input cannot be often guaranteed or expected. Often, such influences are discounted upon developing mathematical methods for general applications. In this manuscript, we carefully examine how such effects would determine the results of spectral similarity-based library search. We show that several popular mathematical spectral matching approaches give incorrect results under the influence of small changes in the baseline and/or the noise. We also discuss the points to be carefully considered while generating a spectral library. We believe our results will be a guiding note for developing applications of Raman spectroscopy that uses a standard spectral library and mathematical spectral matching.

    DOI PubMed

  • High-Quality Draft Genome Sequence of a Rickettsiales Bacterium Found in Acropora tenuis Coral from Okinawa, Japan.

    Keigo Ide, Yohei Nishikawa, Masato Kogawa, Eisuke Iwamoto, Ashok Zachariah Samuel, Yoshikatsu Nakano, Haruko Takeyama

    Microbiology resource announcements   9 ( 48 )  2020年11月  [国際誌]

     概要を見る

    Rickettsiales-like organisms are important for the survival and functioning of corals, prompting an investigation of their complete genomes. Earlier reports of the genomes of these organisms remain incomplete. Here, we report a novel draft genome of Rickettsiales bacterial strain SESOKO1, found in Acropora tenuis coral, using single-cell genome technology.

    DOI PubMed

  • Draft Genome Sequence of Okeania sp. Strain KiyG1, Assembled from Single-Amplified Genomes Collected from Cape Kiyan, Okinawa, Japan.

    Muhammad Wahyudin Lewaru, Yohei Nishikawa, Keigo Ide, Masato Kogawa, Masahito Hosokawa, Ashok Zachariah Samuel, Shinpei Sumimoto, Handung Nuryadi, Shoichiro Suda, Haruko Takeyama

    Microbiology resource announcements   9 ( 46 )  2020年11月  [国際誌]

     概要を見る

    The genus Okeania is a globally distributed group of microorganisms that live in shallow seabed regions. These organisms play several environmentally important roles and are also known producers of several active secondary metabolites with potential human applications. Here, we present a draft genome of Okeania sp. strain KiyG1 (92.7% completeness) that was assembled from four single-amplified genomes.

    DOI PubMed

  • Rapid inspection method for investigating the heat processing conditions employed for chicken meat using Raman spectroscopy

    Rimi Miyaoka, Masahiro Ando, Rieko Harada, Hiroyuki Osaka, Ashok Zachariah Samuel, Masahito Hosokawa, Haruko Takeyama

    Journal of Bioscience and Bioengineering   129 ( 6 ) 700 - 705  2020年06月  [査読有り]  [国内誌]

     概要を見る

    In Japan, the imports of meat products have been increasing every year. Heat processing of meat is the current standard method for ensuring domestic animal health, particularly in case of meat products from areas where infectious diseases are known to have occurred in domestic animals. The Animal Quarantine Service needs to establish a method that detects the temperature at which the meat has been heat-processed (endpoint temperature) to ensure that the standard protocol is followed at the production location. Here, we developed a Raman spectroscopy and multivariate statistics (viz. multivariate curve resolution (MCR))-based simple and rapid method for accurately estimating the end point temperature. We showed that the temperature-dependent secondary structure modification of proteins can serve as an accurate indicator of the temperature of heat processing. This methodology can be easily automated for effective utilization by someone who is not an expert in spectroscopy. We envisage a wider application of this method in food analysis, although the present research investigated the application of this method in chicken meat heat processing analysis.

    DOI PubMed

  • A General Approach for Estimating Lamella-Thickness Distribution in Polymers with Low-Frequency Raman Spectroscopy: Application to Lamella Formation in Crystallizing Polyethylene

    Ashok Zachariah Samuel, Hiro-o Hamaguchi

    Chemistry - A European Journal   24 ( 37 ) 9333 - 9339  2018年07月  [査読有り]

    担当区分:筆頭著者

    DOI

  • Cylindrical micelles of a POSS amphiphilic dendrimer as nano-reactors for polymerization

    Jing-Ting Weng, Tso-Fan Yeh, Ashok Zachariah Samuel, Yi-Fan Huang, Jyun-Hao Sie, Kuan-Yi Wu, Chi-How Peng, Hiro-o Hamaguchi, Chien-Lung Wang

    Nanoscale   10 ( 7 ) 3509 - 3517  2018年  [査読有り]

     概要を見る

    <p>A low-generation amphiphilic dendrimer is applied as a nano-reactor for polymerization for the first time.</p>

    DOI

  • Determination of Percent Crystallinity of Side-Chain Crystallized Alkylated-Dextran Derivatives with Raman Spectroscopy and Multivariate Curve Resolution

    Ashok Zachariah Samuel, Mengbo Zhou, Masahiro Ando, Robert Mueller, Tim Liebert, Thomas Heinze, Hiro-o Hamaguchi

    Analytical Chemistry   88 ( 9 ) 4644 - 4650  2016年05月  [査読有り]

    担当区分:筆頭著者

    DOI

  • Raman and mid-infrared spectroscopic imaging: applications and advancements

    Gautam, R, Ashok Zachariah Samuel, Sil, S, Chaturvedi, D, Dutta, A, Ariese, F, Umapathy, S

      108   341  2015年02月  [査読有り]

  • Stretching Single Polymer Chains of Donor–Acceptor Foldamers: Toward the Quantitative Study on the Extent of Folding

    Kai Liu, Xiaoyan Zheng, Ashok Z. Samuel, S. G. Ramkumar, Suhrit Ghosh, Xinxin Tan, Dong Wang, Zhigang Shuai, S. Ramakrishnan, Dongsheng Liu, Xi Zhang

    Langmuir   29 ( 47 ) 14438 - 14443  2013年11月  [査読有り]

    DOI

  • Self-Adapting Peripherally Heterofunctionalized Hyperbranched Polymers: Formation of Janus and Tripodal Structures

    Ashok Zachariah Samuel, S. Ramakrishnan

    Langmuir   29 ( 4 ) 1245 - 1257  2013年01月  [査読有り]

    担当区分:筆頭著者

    DOI

  • 2D Correlation Raman Spectroscopy of Hyperbranched Polymer

    Ashok Z Samuel, S Ramakrishnan, S Umapathy

    AIP Conference Proceedings    2010年

    担当区分:筆頭著者

▼全件表示

Misc

  • 顕微ラマン分光法を用いた生理活性物質Mangromicin類縁体の検出

    向島諒, 安藤正浩, 安藤正浩, ASHOK Samuel, 中島琢自, 高橋洋子, 松本厚子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本化学会春季年会講演予稿集(CD-ROM)   100th  2020年

    J-GLOBAL

  • 顕微ラマン多変量スペクトル分解法を用いた二次代謝産物penicillin及びavermectinの菌体内検出

    堀井俊平, 堀井俊平, 安藤正弘, 安藤正弘, 中島琢自, 中島琢自, ASHOK Samuel, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本農芸化学会大会講演要旨集(Web)   2020  2020年

    J-GLOBAL

  • 顕微ラマン多変量スペクトル分解法を用いた生理活性物質penicillin及びavermectinの菌体内検出

    堀井俊平, 安藤正浩, 安藤正浩, 武晃, 武晃, SAMUEL Ashok, 中島琢自, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子

    日本化学会春季年会講演予稿集(CD-ROM)   99th  2019年

    J-GLOBAL

  • 顕微ラマン分光法及び多変量スペクトル分解法を用いた生理活性物質penicillin及びavermectinの菌体内検出

    堀井俊平, 堀井俊平, 安藤正浩, 安藤正浩, 中島琢自, ASHOK Samuel, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

    日本生物工学会大会講演要旨集   71st  2019年

    J-GLOBAL

Works(作品等)

  • Creations of illusion (https://www.blurb.com/b/7348092-creations-of-illusion)

    Ashok Zachariah Samuel  芸術活動 

    2016年
    -
     

受賞

  • Dr. J C GHOSH MEDAL

    2014年03月   Indian Institute of Science  

  • EARLY CAREER RESEARCHER AWARD

    2013年10月   Society for Applied Spectroscopy, USA  

  • JUNIOR RESEARCH FELLOWSHIP

    2005年06月  

  • University First Rank

    2003年05月  

講演・口頭発表等

  • 細胞内核酸:ゲノム編集までのスカラ自然、およびそれを超えて

     [招待有り]

    発表年月: 2022年03月

  • Extrusion of extracellular vesicles (EVs) containing a specific secondary metabolite by Penicillium chrysogenum

    Samuel, Ashok Zachariah

    15th Symposium on Biorelevant Chemistry  

    発表年月: 2021年09月

  • A brief journey through Raman Spectroscopy

    Samuel, Ashok Zachariah  [招待有り]

    Analytical Tools in Chemistry  

    発表年月: 2021年08月

  • Extracellular Vesicles Mediate the Release of the Secondary Metabolite Produced by Penicillium chrysogenum

    Samuel, Ashok Zachariah

    World Microbe Forum 2021  

    発表年月: 2021年06月

  • Molecular profiling of lipid droplets inside HuH7 human liver cells with Raman micro-spectroscopy

    Ashok Zachariah Samuel

    5th Core-to-Core International Symposium, Okinawa Institute of Science and Technology, Okinawa, Japan  

    発表年月: 2019年

  • Critical analysis of quantitative molecular profiling of lipid droplets in HuH7cells with Raman micro-spectroscopy and multivariate curve resolution

    Ashok Zachariah Samuel

    10th International Conference on Advanced Vibrational Spectroscopy (ICAVS 10), Auckland, New Zealand  

    発表年月: 2019年

  • Low-frequency Longitudinal Acoustic Modes in Polyethylene Reveals Mechanistic Aspects of Polymer Lamella Formation

    Ashok Zachariah Samuel

    5th Taiwan International Symposium on Raman Spectroscopy (TISRS), Chiayi, Taiwan.  

    発表年月: 2017年

  • Quantitative Elucidation of Molecular Chain-Folding in Polymers with Low-frequency Raman Spectroscopy

    Ashok Zachariah Samuel

    The Asian Spectroscopy Conference, Taiwan  

    発表年月: 2017年

  • Molecular Structural Re-organization at Single Molecule Level and High-Speed Raman Imaging with 2D Array Multifocus Microscopy

    Ashok Zachariah Samuel  [招待有り]

    MG University, CMS College, INDIA  

    発表年月: 2017年

  • Absolute Percent Crystallinity of Polyethylene with Raman Spectroscopy and Multivariate Curve Resolution by Alternating Least Squares (MCR-ALS)

    Ashok Zachariah Samuel

    4th Taiwan International Symposium on Raman Spectroscopy, Taipei, Taiwan  

    発表年月: 2016年

  • Determination of Absolute Values of Physical Properties of Polymers and Examining Interfacial Reactions in Molecular Gels with Raman microspectroscopy

    Ashok Zachariah Samuel  [招待有り]

    Indian Institute of Technology, Kanpur (IIT Kanpur), INDIA  

    発表年月: 2016年

  • Absolute Percent Crystallinity Determination with Raman Spectroscopy

    Ashok Zachariah Samuel

    3rd Taiwan International Symposium on Raman Spectroscopy, Taiwan  

    発表年月: 2015年

  • Determination of Absolute Values of Physical Properties of Polymers and Examining Water Structure in Micelles

    Ashok Zachariah Samuel  [招待有り]

    Hyderabad Central University (HCU), INDIA  

    発表年月: 2015年

  • Raman Spectroscopic Investigations of Surfactant Gelation Induced by Aromatic Carboxylic Acids and Their Salts: A Molecular Perspective

    Ashok Zachariah Samuel

    The Chemical Society of Japan 94th Spring Annual Meeting, Nagoya University, Japan  

    発表年月: 2014年

  • Microstructure Characterization of Porous PMMA Thin films with Multifocus Raman Microscopy

    Ashok Zachariah Samuel

    The Chemical;Society of Ja;Spring Annual Meeting;Nagoya University, Japan  

    発表年月: 2014年

  • Unusual Acid-Base Equilibrium of an Aromatic Carboxylic Acid at the Interface

    Ashok Zachariah Samuel  [招待有り]

    The Second Taiwan International Symposium on Raman Spectroscopy, 2014, National Dong Hwa University, Hualien-Taiwan  

    発表年月: 2014年

  • Raman Spectroscopic Investigations of Surfactant Gels

    Ashok Zachariah Samuel

    33rd International Conference on Solution Chemistry (ICSC) post-symposium on Ionic Liquids from Science to Green Chemical Applications  

    発表年月: 2013年

  • Morphology of Porous Polymer Film Studied with Multifocus Raman Microspectroscopy

    Ashok Zachariah Samuel

    SCIX International Conference, Milwaukee USA  

    発表年月: 2013年

  • Intermolecular Interaction in Self-Assembled Soft Transparent-Gels Investigated with Low Wavenumber Raman Spectroscopy

    Ashok Zachariah Samuel

    7th International Conference on Advanced Vibrational Spectroscopy (ICAVS), Kobe, Japan  

    発表年月: 2013年

  • Gelation of Aqueous Surfactant Solutions Examined with Raman Spectroscopy

    Ashok Zachariah Samuel

    Annual Meeting of Japan Society for Molecular Science, Kyoto, Japan  

    発表年月: 2013年

  • Intermolecular Interaction in Transparent Surfactant Gels Examined Using a Low-wavenumber Raman Microspectrometer

    Ashok Zachariah Samuel

    SCIX International Conference, Milwaukee USA  

    発表年月: 2013年

  • Raman spectroscopic investigation of transparent surfactant gels: unexpected behavior of an aromatic carboxylic acid

    Ashok Zachariah Samuel  [招待有り]

    Mejiro One-day Symposium on Advanced Spectroscopy -Structure and Dynamics, Gakushuin University, Japan  

    発表年月: 2013年

  • Unusual Behaviour of an Aromatic Carboxylic Acid in Surfactant Gels Studied with Multifocus Raman Microscopy - a “Super-Fast” Raman Imaging Alternative

    Ashok Zachariah Samuel  [招待有り]

    Frontiers of Advanced Laser Spectroscopy, RIKEN, Japan  

    発表年月: 2013年

  • Cascading Configurations of Polymer Segments: A 2D Raman Correlation Study of Melting PEG

    Ashok Zachariah Samuel

    23rd International Conference on Raman Spectroscopy (ICORS), India  

    発表年月: 2012年

  • Janus Hybramers: A Simple Way to Janus Molecular Architectures Based on Self Adapting Amphiphilic Hyperbranched Polymers

    Ashok Zachariah Samuel

    Indo-US Bilateral Workshop On Nanoparticle Assembly  

    発表年月: 2011年

  • 2D Correlation Raman Spectroscopy of Hyperbranched Polymer

    Ashok Zachariah Samuel

    XXII International Conference On Raman Spectroscopy, Boston, USA  

    発表年月: 2010年

▼全件表示

 

担当経験のある科目(授業)

  • 光ラマン分光法:分子に敏感な単一細胞生物学の黎明期

    早稲田大学  

    2021年10月
     
     
     

  • 化学の分析ツール:ラマン分光法の簡単な旅

    カリカット大学化学科  

    2021年08月
     
     
     

  • Raman spectroscopy (Fundamentals and applications)

    Waseda University  

    2019年03月
    -
    2019年04月
     

 

学術貢献活動

  • Reviews articles for major journals (Chem. Comm, Sci. Rep., Sci. Adv., J. Raman. Spectrosc., Spectroscopy Acta etc.)

    査読等