Details of a Researcher - SAMUEL, Ashok Zachariah

写真a

SAMUEL, Ashok Zachariah

Affiliation

Research Council (Research Organization), Research Organization for Nano & Life Innovation

Job title

Junior Researcher(Assistant Professor)

Education

2006.01

-

2012.07

Indian Institute of Science

Research Experience

2017.11

-

Now

Waseda University
2014.04

-

2017.10

National Chiao Tung University
2013.01

-

2014.03

Gakushuin University Department of chemistry

Professional Memberships

2019

-

2020

International Marine Biotechnology Association
2019

-

　

Chemical Society of Japan
2014

-

2015

Chemical Society of Japan
2013.10

-

2014.10

Society for Applied Spectroscopy (SAS)

Research Areas

Basic analysis
Molecular biology Raman Imaging, Multivariate analysis

Research Interests

Developing analytical methods for general applications of Raman spectroscopy
Raman imaging of biological systems
Mid IR imaging
Fluorescence imaging
Raman instrumentation
PCA, MCR, SVD, Cluster analysis, 2D correlation analysis
Single cell imaging
Polymers
Biophotonics (Spectral Imaging)
Multivariate statistical analyses of spectral data
Raman spectroscopy

▼display all

Papers

Raman Microspectroscopy Imaging Analysis of Extracellular Vesicles Biogenesis by Filamentous Fungus Penicilium chrysogenum

Ashok Zachariah Samuel, Shumpei Horii, Takuji Nakashima, Naoko Shibata, Masahiro Ando, Haruko Takeyama

Advanced Biology 2101322 - 2101322 2022.03 [Refereed]

Authorship：Lead author

DOI DOI2
Deconstruction of Obscure Features in SVD-Decomposed Raman Images from P. chrysogenum Reveals Complex Mixing of Spectra from Five Cellular Constituents

Ashok Zachariah Samuel, Shumpei Horii, Masahiro Ando, Haruko Takeyama

Analytical Chemistry 93 ( 35 ) 12139 - 12146 2021.09

Authorship：Lead author, Corresponding author

DOI
Molecular profiling of lipid droplets inside HuH7 cells with Raman micro-spectroscopy

Ashok Zachariah Samuel, Rimi Miyaoka, Masahiro Ando, Anne Gaebler, Christoph Thiele, Haruko Takeyama

Communications Biology 3 ( 1 ) 372 - 372 2020.12 [Refereed] [International journal]

Authorship：Lead author

　View Summary

Raman imaging has become an attractive technology in molecular biology because of its ability to detect multiple molecular components simultaneously without labeling. Two major limitations in accurately accounting for spectral features, viz., background removal and spectral unmixing, have been overcome by employing a modified and effective routine in multivariate curve resolution (MCR). With our improved strategy, we have spectrally isolated seven structurally specific biomolecules without any post-acquisition spectral treatments. Consequently, the isolated intensity profiles reflected concentrations of corresponding biomolecules with high statistical accuracy. Our study reveals the changes in the molecular composition of lipid droplets (LDs) inside HuH7 cells and its relation to the physiological state of the cell. Further, we show that the accurate separation of spectral components permits analysis of structural modification of molecules after cellular uptake. A detailed discussion is presented to highlight the potential of Raman spectroscopy with MCR in semi-quantitative molecular profiling of living cells.

DOI PubMed
Detection of Penicillin G Produced by Penicillium chrysogenum KF 425 in Vivo with Raman Microspectroscopy and Multivariate Curve Resolution-Alternating Least Squares Methods

Shumpei Horii, Masahiro Ando, Ashok Z. Samuel, Akira Take, Takuji Nakashima, Atsuko Matsumoto, Yōko Takahashi, Haruko Takeyama

J. Nat. Prod. Accepted 2020.03 [Refereed]

　View Summary

<title>ABSTRACT</title>Raman microspectroscopy is a minimally invasive technique that can identify molecular structure without labeling. In this study, we demonstrate in vivo detection of the bioactive compound penicillin G inside <italic>Penicillium chrysogenum</italic> KF425 fungus cells. Highly overlapped spectroscopic signatures acquired using Raman microspectroscopic imaging are analyzed using a multivariate curve resolution-alternating least squares (MCR-ALS) method to extract the pure spectra of individual molecular constituents. In addition to detecting multiple constituents such as proteins and lipids, we observe the subcellular localization of penicillin G like granule particle inside the fungus body. To date, there have been no reports of direct visualization of intracellular localization of penicillin G. The methodology we present in this article is expected to be applied as a screening tool for the production of bioactive compounds by microorganisms.

DOI
Direct estimation of polymer crystallinity with Raman spectroscopy using ratio of scattering cross-sections estimated from variable temperature measurements

Ashok Zachariah Samuel

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 224 117431 - 117431 2020.01 [Refereed]

Authorship：Lead author, Corresponding author

DOI
Estimating Percent Crystallinity of Polyethylene as a Function of Temperature by Raman Spectroscopy Multivariate Curve Resolution by Alternating Least Squares

Ashok Zachariah Samuel, Bo-Han Lai, Shih-Ting Lan, Masahiro Ando, Chien-Lung Wang, Hiro-o Hamaguchi

Analytical Chemistry 89 ( 5 ) 3043 - 3050 2017.03 [Refereed]

Authorship：Lead author

DOI
Rapid microstructure characterization of polymer thin films with 2D-array multifocus Raman microspectroscopy

Ashok Zachariah Samuel, Sohshi Yabumoto, Kenichi Kawamura, Koichi Iwata

The Analyst 140 ( 6 ) 1847 - 1851 2015 [Refereed]

Authorship：Lead author

　View Summary

<p>Multifocus Raman imaging is one of the fast-imaging alternatives to the conventional single point mapping technique.</p>

DOI
Energy funneling and macromolecular conformational dynamics: a 2D Raman correlation study of PEG melting

Ashok Zachariah Samuel, Siva Umapathy

Polymer Journal 46 ( 6 ) 330 - 336 2014.06 [Refereed]

Authorship：Lead author

DOI
Conformational Reorganization of Hyperbranched and Linear Polymers and Functionalized Porous Polymer Films

Ashok Zachariah Samuel

2014 [Refereed]

Authorship：Lead author
Janus Hybramers: Self-Adapting Amphiphilic Hyperbranched Polymers

Ashok Zachariah Samuel, S. Ramakrishnan

Macromolecules 45 ( 5 ) 2348 - 2358 2012.03 [Refereed]

Authorship：Lead author

DOI
Functionalized and Postfunctionalizable Porous Polymeric Films through Evaporation-Induced Phase Separation Using Mixed Solvents

Ashok Zachariah Samuel, S. Umapathy, S. Ramakrishnan

ACS Applied Materials & Interfaces 3 ( 9 ) 3293 - 3299 2011.09 [Refereed]

Authorship：Lead author

DOI
On Selecting a Suitable Spectral Matching Method for Automated Analytical Applications of Raman Spectroscopy.

Ashok Zachariah Samuel, Ryo Mukojima, Shumpei Horii, Masahiro Ando, Soshi Egashira, Takuji Nakashima, Masato Iwatsuki, Haruko Takeyama

ACS omega 6 ( 3 ) 2060 - 2065 2021.01 [International journal]

Authorship：Lead author, Corresponding author

　View Summary

Raman spectra are molecular structure-specific and hence are employed in applications requiring chemical identification. The advent of efficient handheld and smartphone-based Raman instruments is promoting widespread applications of the technique, which often involve less trained end users. Software modules that enable spectral library searches based on spectral pattern matching is an essential part of such applications. The Raman spectrum recorded by end users will naturally have varying levels of signal to noise (SN), baseline fluctuations, etc., depending on the sample environment. Further, in biological, forensic, food, pharmaceuticals, etc., fields where a vast amount of Raman spectral data is generated, careful removal of background is often impossible. In other words, a 100% match between the library spectrum and user input cannot be often guaranteed or expected. Often, such influences are discounted upon developing mathematical methods for general applications. In this manuscript, we carefully examine how such effects would determine the results of spectral similarity-based library search. We show that several popular mathematical spectral matching approaches give incorrect results under the influence of small changes in the baseline and/or the noise. We also discuss the points to be carefully considered while generating a spectral library. We believe our results will be a guiding note for developing applications of Raman spectroscopy that uses a standard spectral library and mathematical spectral matching.

DOI PubMed
High-Quality Draft Genome Sequence of a Rickettsiales Bacterium Found in Acropora tenuis Coral from Okinawa, Japan.

Keigo Ide, Yohei Nishikawa, Masato Kogawa, Eisuke Iwamoto, Ashok Zachariah Samuel, Yoshikatsu Nakano, Haruko Takeyama

Microbiology resource announcements 9 ( 48 ) 2020.11 [International journal]

　View Summary

Rickettsiales-like organisms are important for the survival and functioning of corals, prompting an investigation of their complete genomes. Earlier reports of the genomes of these organisms remain incomplete. Here, we report a novel draft genome of Rickettsiales bacterial strain SESOKO1, found in Acropora tenuis coral, using single-cell genome technology.

DOI PubMed
Draft Genome Sequence of Okeania sp. Strain KiyG1, Assembled from Single-Amplified Genomes Collected from Cape Kiyan, Okinawa, Japan.

Muhammad Wahyudin Lewaru, Yohei Nishikawa, Keigo Ide, Masato Kogawa, Masahito Hosokawa, Ashok Zachariah Samuel, Shinpei Sumimoto, Handung Nuryadi, Shoichiro Suda, Haruko Takeyama

Microbiology resource announcements 9 ( 46 ) 2020.11 [International journal]

　View Summary

The genus Okeania is a globally distributed group of microorganisms that live in shallow seabed regions. These organisms play several environmentally important roles and are also known producers of several active secondary metabolites with potential human applications. Here, we present a draft genome of Okeania sp. strain KiyG1 (92.7% completeness) that was assembled from four single-amplified genomes.

DOI PubMed
Rapid inspection method for investigating the heat processing conditions employed for chicken meat using Raman spectroscopy

Rimi Miyaoka, Masahiro Ando, Rieko Harada, Hiroyuki Osaka, Ashok Zachariah Samuel, Masahito Hosokawa, Haruko Takeyama

Journal of Bioscience and Bioengineering 129 ( 6 ) 700 - 705 2020.06 [Refereed] [Domestic journal]

　View Summary

In Japan, the imports of meat products have been increasing every year. Heat processing of meat is the current standard method for ensuring domestic animal health, particularly in case of meat products from areas where infectious diseases are known to have occurred in domestic animals. The Animal Quarantine Service needs to establish a method that detects the temperature at which the meat has been heat-processed (endpoint temperature) to ensure that the standard protocol is followed at the production location. Here, we developed a Raman spectroscopy and multivariate statistics (viz. multivariate curve resolution (MCR))-based simple and rapid method for accurately estimating the end point temperature. We showed that the temperature-dependent secondary structure modification of proteins can serve as an accurate indicator of the temperature of heat processing. This methodology can be easily automated for effective utilization by someone who is not an expert in spectroscopy. We envisage a wider application of this method in food analysis, although the present research investigated the application of this method in chicken meat heat processing analysis.

DOI PubMed
A General Approach for Estimating Lamella-Thickness Distribution in Polymers with Low-Frequency Raman Spectroscopy: Application to Lamella Formation in Crystallizing Polyethylene

Ashok Zachariah Samuel, Hiro-o Hamaguchi

Chemistry - A European Journal 24 ( 37 ) 9333 - 9339 2018.07 [Refereed]

Authorship：Lead author

DOI
Cylindrical micelles of a POSS amphiphilic dendrimer as nano-reactors for polymerization

Jing-Ting Weng, Tso-Fan Yeh, Ashok Zachariah Samuel, Yi-Fan Huang, Jyun-Hao Sie, Kuan-Yi Wu, Chi-How Peng, Hiro-o Hamaguchi, Chien-Lung Wang

Nanoscale 10 ( 7 ) 3509 - 3517 2018 [Refereed]

　View Summary

<p>A low-generation amphiphilic dendrimer is applied as a nano-reactor for polymerization for the first time.</p>

DOI
Determination of Percent Crystallinity of Side-Chain Crystallized Alkylated-Dextran Derivatives with Raman Spectroscopy and Multivariate Curve Resolution

Ashok Zachariah Samuel, Mengbo Zhou, Masahiro Ando, Robert Mueller, Tim Liebert, Thomas Heinze, Hiro-o Hamaguchi

Analytical Chemistry 88 ( 9 ) 4644 - 4650 2016.05 [Refereed]

Authorship：Lead author

DOI
Raman and mid-infrared spectroscopic imaging: applications and advancements

Gautam, R, Ashok Zachariah Samuel, Sil, S, Chaturvedi, D, Dutta, A, Ariese, F, Umapathy, S

108 341 2015.02 [Refereed]
Stretching Single Polymer Chains of Donor–Acceptor Foldamers: Toward the Quantitative Study on the Extent of Folding

Kai Liu, Xiaoyan Zheng, Ashok Z. Samuel, S. G. Ramkumar, Suhrit Ghosh, Xinxin Tan, Dong Wang, Zhigang Shuai, S. Ramakrishnan, Dongsheng Liu, Xi Zhang

Langmuir 29 ( 47 ) 14438 - 14443 2013.11 [Refereed]

DOI
Self-Adapting Peripherally Heterofunctionalized Hyperbranched Polymers: Formation of Janus and Tripodal Structures

Ashok Zachariah Samuel, S. Ramakrishnan

Langmuir 29 ( 4 ) 1245 - 1257 2013.01 [Refereed]

Authorship：Lead author

DOI
2D Correlation Raman Spectroscopy of Hyperbranched Polymer

Ashok Z Samuel, S Ramakrishnan, S Umapathy

AIP Conference Proceedings 2010

Authorship：Lead author

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Misc

Detection of Mangromicin analogs by using Raman microspectroscopy

向島諒, 安藤正浩, 安藤正浩, ASHOK Samuel, 中島琢自, 高橋洋子, 松本厚子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

日本化学会春季年会講演予稿集(CD-ROM) 100th 2020

J-GLOBAL
In situ Detection of Penicillin and Avermectin in Microbes by Raman Microspectroscopy and Multivariate Analysis

堀井俊平, 堀井俊平, 安藤正弘, 安藤正弘, 中島琢自, 中島琢自, ASHOK Samuel, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

日本農芸化学会大会講演要旨集(Web) 2020 2020

J-GLOBAL
顕微ラマン多変量スペクトル分解法を用いた生理活性物質penicillin及びavermectinの菌体内検出

堀井俊平, 安藤正浩, 安藤正浩, 武晃, 武晃, SAMUEL Ashok, 中島琢自, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子

日本化学会春季年会講演予稿集(CD-ROM) 99th 2019

J-GLOBAL
顕微ラマン分光法及び多変量スペクトル分解法を用いた生理活性物質penicillin及びavermectinの菌体内検出

堀井俊平, 堀井俊平, 安藤正浩, 安藤正浩, 中島琢自, ASHOK Samuel, 松本厚子, 高橋洋子, 竹山春子, 竹山春子, 竹山春子, 竹山春子

日本生物工学会大会講演要旨集 71st 2019

J-GLOBAL

Works

Creations of illusion (https://www.blurb.com/b/7348092-creations-of-illusion)

Ashok Zachariah Samuel Artistic work

2016

-

Awards

Dr. J C GHOSH MEDAL

2014.03 Indian Institute of Science
EARLY CAREER RESEARCHER AWARD

2013.10 Society for Applied Spectroscopy, USA
JUNIOR RESEARCH FELLOWSHIP

2005.06
University First Rank

2003.05

Presentations

INTRACELLULAR NUCLEIC ACIDS: SCALA NATURAE TO GENE EDITING, AND BEYOND

Ashok Zachariah Samuel [Invited]

Webinar, Department of Forensic Science, Garden city University, INDIA

Presentation date： 2022.03
Extrusion of extracellular vesicles (EVs) containing a specific secondary metabolite by Penicillium chrysogenum

Samuel, Ashok Zachariah

15th Symposium on Biorelevant Chemistry

Presentation date： 2021.09
A brief journey through Raman Spectroscopy

Samuel, Ashok Zachariah [Invited]

Analytical Tools in Chemistry

Presentation date： 2021.08
Extracellular Vesicles Mediate the Release of the Secondary Metabolite Produced by Penicillium chrysogenum

Samuel, Ashok Zachariah

World Microbe Forum 2021

Presentation date： 2021.06
Molecular profiling of lipid droplets inside HuH7 human liver cells with Raman micro-spectroscopy

Ashok Zachariah Samuel

5th Core-to-Core International Symposium, Okinawa Institute of Science and Technology, Okinawa, Japan

Presentation date： 2019
Critical analysis of quantitative molecular profiling of lipid droplets in HuH7cells with Raman micro-spectroscopy and multivariate curve resolution

Ashok Zachariah Samuel

10th International Conference on Advanced Vibrational Spectroscopy (ICAVS 10), Auckland, New Zealand

Presentation date： 2019
Low-frequency Longitudinal Acoustic Modes in Polyethylene Reveals Mechanistic Aspects of Polymer Lamella Formation

Ashok Zachariah Samuel

5th Taiwan International Symposium on Raman Spectroscopy (TISRS), Chiayi, Taiwan.

Presentation date： 2017
Quantitative Elucidation of Molecular Chain-Folding in Polymers with Low-frequency Raman Spectroscopy

Ashok Zachariah Samuel

The Asian Spectroscopy Conference, Taiwan

Presentation date： 2017
Molecular Structural Re-organization at Single Molecule Level and High-Speed Raman Imaging with 2D Array Multifocus Microscopy

Ashok Zachariah Samuel [Invited]

MG University, CMS College, INDIA

Presentation date： 2017
Absolute Percent Crystallinity of Polyethylene with Raman Spectroscopy and Multivariate Curve Resolution by Alternating Least Squares (MCR-ALS)

Ashok Zachariah Samuel

4th Taiwan International Symposium on Raman Spectroscopy, Taipei, Taiwan

Presentation date： 2016
Determination of Absolute Values of Physical Properties of Polymers and Examining Interfacial Reactions in Molecular Gels with Raman microspectroscopy

Ashok Zachariah Samuel [Invited]

Indian Institute of Technology, Kanpur (IIT Kanpur), INDIA

Presentation date： 2016
Absolute Percent Crystallinity Determination with Raman Spectroscopy

Ashok Zachariah Samuel

3rd Taiwan International Symposium on Raman Spectroscopy, Taiwan

Presentation date： 2015
Determination of Absolute Values of Physical Properties of Polymers and Examining Water Structure in Micelles

Ashok Zachariah Samuel [Invited]

Hyderabad Central University (HCU), INDIA

Presentation date： 2015
Raman Spectroscopic Investigations of Surfactant Gelation Induced by Aromatic Carboxylic Acids and Their Salts: A Molecular Perspective

Ashok Zachariah Samuel

The Chemical Society of Japan 94th Spring Annual Meeting, Nagoya University, Japan

Presentation date： 2014
Microstructure Characterization of Porous PMMA Thin films with Multifocus Raman Microscopy

Ashok Zachariah Samuel

The Chemical;Society of Ja;Spring Annual Meeting;Nagoya University, Japan

Presentation date： 2014
Unusual Acid-Base Equilibrium of an Aromatic Carboxylic Acid at the Interface

Ashok Zachariah Samuel [Invited]

The Second Taiwan International Symposium on Raman Spectroscopy, 2014, National Dong Hwa University, Hualien-Taiwan

Presentation date： 2014
Raman Spectroscopic Investigations of Surfactant Gels

Ashok Zachariah Samuel

33rd International Conference on Solution Chemistry (ICSC) post-symposium on Ionic Liquids from Science to Green Chemical Applications

Presentation date： 2013
Morphology of Porous Polymer Film Studied with Multifocus Raman Microspectroscopy

Ashok Zachariah Samuel

SCIX International Conference, Milwaukee USA

Presentation date： 2013
Intermolecular Interaction in Self-Assembled Soft Transparent-Gels Investigated with Low Wavenumber Raman Spectroscopy

Ashok Zachariah Samuel

7th International Conference on Advanced Vibrational Spectroscopy (ICAVS), Kobe, Japan

Presentation date： 2013
Gelation of Aqueous Surfactant Solutions Examined with Raman Spectroscopy

Ashok Zachariah Samuel

Annual Meeting of Japan Society for Molecular Science, Kyoto, Japan

Presentation date： 2013
Intermolecular Interaction in Transparent Surfactant Gels Examined Using a Low-wavenumber Raman Microspectrometer

Ashok Zachariah Samuel

SCIX International Conference, Milwaukee USA

Presentation date： 2013
Raman spectroscopic investigation of transparent surfactant gels: unexpected behavior of an aromatic carboxylic acid

Ashok Zachariah Samuel [Invited]

Mejiro One-day Symposium on Advanced Spectroscopy -Structure and Dynamics, Gakushuin University, Japan

Presentation date： 2013
Unusual Behaviour of an Aromatic Carboxylic Acid in Surfactant Gels Studied with Multifocus Raman Microscopy - a “Super-Fast” Raman Imaging Alternative

Ashok Zachariah Samuel [Invited]

Frontiers of Advanced Laser Spectroscopy, RIKEN, Japan

Presentation date： 2013
Cascading Configurations of Polymer Segments: A 2D Raman Correlation Study of Melting PEG

Ashok Zachariah Samuel

23rd International Conference on Raman Spectroscopy (ICORS), India

Presentation date： 2012
Janus Hybramers: A Simple Way to Janus Molecular Architectures Based on Self Adapting Amphiphilic Hyperbranched Polymers

Ashok Zachariah Samuel

Indo-US Bilateral Workshop On Nanoparticle Assembly

Presentation date： 2011
2D Correlation Raman Spectroscopy of Hyperbranched Polymer

Ashok Zachariah Samuel

XXII International Conference On Raman Spectroscopy, Boston, USA

Presentation date： 2010

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Teaching Experience

Optical Raman spectroscopy: The dawn of molecularly sensitive single-cell biology

Waseda University
2021.10

　

　
Analytical Tools in Chemistry: A brief journey through Raman Spectroscopy

The Department of Chemistry, University of Calicut
2021.08

　

　
Raman spectroscopy (Fundamentals and applications)

Waseda University
2019.03

-

2019.04

Academic Activities

Reviews articles for major journals (Chem. Comm, Sci. Rep., Sci. Adv., J. Raman. Spectrosc., Spectroscopy Acta etc.)

Peer review