2022/01/19 更新

写真a

ニュウノヤ ヒロシ
丹生谷 博
所属
理工学術院 創造理工学部
職名
講師(任期付)

学歴

  •  
    -
    1981年09月

    東京大学大学院理学系研究科博士課程修了(理学博士)  

  • 1975年04月
    -
    1980年03月

    東京大学大学院   理学系研究科   植物学専攻  

    満期退学

  • 1971年04月
    -
    1975年03月

    京都大学   農学部   農林生物学科  

    卒業

学位

  • 東京大学   理学博士

経歴

  • 2017年04月
    -
    継続中

    早稲田大学理工学術院   創造理工学部(実験教育センター)   講師(任期付)

  • 2017年03月
    -
    継続中

    明治大学   経営学部   兼任講師

  • 1995年04月
    -
    2017年03月

    東京農工大学   遺伝子実験施設   助教授 ー 教授

  • 1995年10月
     
     

    (独)マックスプランク研究所(ポツダム)   分子植物生理学研究所   文科省在外研究員

  • 1986年11月
    -
    1995年03月

    国立がんセンター研究所   ウイルス部   研究員 ー 主任研究官

  • 1989年09月
    -
    1989年11月

    (独)エアランゲン・ニュルンベルク大学   臨床分子ウイルス学研究所   共同研究員

  • 1986年02月
    -
    1986年11月

    国立がんセンターリサーチレジデント(癌研究振興会非常勤職員)

  • 1980年04月
    -
    1985年10月

    岡山大学理学部   助手

  • 1982年06月
    -
    1985年06月

    Public Health Research Institute of The City of New York   訪問研究員

▼全件表示

 

研究分野

  • 植物分子、生理科学

  • 植物保護科学

研究キーワード

  • 植物と植物ウイルスの相互作用

  • Interaction between plant and plant virus

論文

  • Cooperative roles of introns 1 and 2 of tobacco resistance gene N in enhanced N transcript expression and antiviral defense responses

    Chihiro Ikeda, Kazuo Taku, Tsumugi Miyazaki, Rikako Shirai, Richard S. Nelson, Hiroshi Nyunoya, Yasuhiko Matsushita, Nobumitsu Sasaki

    Scientific Reports   11 ( 15424 )  2021年12月  [査読有り]

     概要を見る

    <title>Abstract</title>The tobacco virus resistance gene <italic>N</italic> contains four introns. Transient expression of transcripts from an <italic>N</italic> transgene containing these introns and driven by the native promoter in the presence of the elicitor of tobacco mosaic virus resulted in its increased expression. The requirement of the native promoter, the elicitor, or the individual introns for enhanced expression of <italic>N</italic> has not been fully studied. Here, we determined that 35S promoter-driven <italic>N</italic> transcript expression could be enhanced in the presence of the four introns regardless of the co-expression of the virus elicitor in tobacco. Function analyses using a series of <italic>N</italic> transgenes with different combination of introns revealed that the presence of intron 1 more so than intron 2 allowed higher accumulation of premature and mature <italic>N</italic> transcripts; however, both introns were important for not only enhanced gene expression but also for induction of cell death in tobacco and induced local resistance to spread of virus in <italic>Nicotiana benthamiana</italic>. Our findings indicate that introns 1 and 2 cooperatively contribute to <italic>N</italic> expression and virus resistance.

    DOI

  • Cell-death-independent antiviral response mediated by N resistance factor in Nicotiana benthamiana involves inhibited localization of tobamovirus movement protein to plasmodesmata

    Nobumitsu Sasaki, Tomoya Murakami, Nanae Yoshimoto, Ken Komatsu, Yasuhiko Matsushita, Hiroshi Nyunoya

    Journal of General Plant Pathology   87 ( 3 ) 170 - 177  2021年05月  [査読有り]

    担当区分:最終著者

    DOI

  • Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement: Far-Western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein.

    Sasaki N, Matsushita Y, Nyunoya H

    In "(book) Antiviral Resistance in Plants : Methods and Protocols", Methods in Molecular Biology   ( 2028 ) 123 - 144  2019年06月  [査読有り]  [招待有り]

    DOI

  • Altered subcellular localization of a tobacco membrane raft-associated remorin protein by tobamovirus infection and transient expression of viral replication and movement proteins

    Nobumitsu Sasaki, Eita Takashima, Hiroshi Nyunoya

    Frontiers in Plant Science   9   619  2018年05月  [査読有り]

     概要を見る

    Remorins are plant specific proteins found in plasma membrane microdomains (termed lipid or membrane rafts) and plasmodesmata. A potato remorin is reported to be involved in negatively regulating potexvirus movement and plasmodesmal permeability. In this study, we isolated cDNAs of tobacco remorins (NtREMs) and examined roles of an NtREM in infection by tomato mosaic virus (ToMV). Subcellular localization analysis using fluorescently tagged NtREM, ToMV, and viral replication and movement proteins (MPs) indicated that virus infection and transient expression of the viral proteins promoted the formation of NtREM aggregates by altering the subcellular distribution of NtREM, which was localized uniformly on the plasma membrane under normal conditions. NtREM aggregates were often observed associated closely with endoplasmic reticulum networks and bodies of the 126K replication and MPs. The bimolecular fluorescence complementation assay indicated that NtREM might interact directly with the MP on the plasma membrane and around plasmodesmata. In addition, transient overexpression of NtREM facilitated ToMV cell-to-cell movement. Based on these results, we discuss possible roles of the tobacco remorin in tobamovirus movement.

    DOI PubMed

  • Introns of the tobacco resistance gene N play important roles in elicitor-responsive upregulation and efficient induction of defense responses

    Kazuo Taku, Nobumitsu Sasaki, Kenta Matsuzawa, Atsushi Okamura-Mukai, Hiroshi Nyunoya

    Journal of General Plant Pathology   84 ( 2 ) 73 - 84  2018年03月  [査読有り]

     概要を見る

    The tobacco resistance gene N is known to be upregulated transcriptionally upon infection with Tobacco mosaic virus or the transient expression of the virus elicitor (termed p50), which is the helicase domain of the virus replicase, leading to cell death-associated defense responses. To investigate the roles of the encoded protein and introns of the N gene in its elicitor-triggered upregulation, we developed an Agrobacterium-mediated transient gene transfer system to express the N transgene controlled by the upstream region of the N gene itself in N-lacking tobacco. The transcript level of the N transgene with the introns was enhanced sharply when the p50 transgene was coexpressed. Introduction of a frameshift mutation in the intron-containing N transgene abolished its response to the elicitor, but an additional expression of the functional N protein supplied in trans complemented the upregulation of the mutant transgene. In contrast, an intron-less N transgene failed to be upregulated by the p50 coexpression, resulting in delayed and attenuated cell death and slow induction of defense-related gene expression. In addition, introduction of a frameshift mutation in the intron-less N transgene resulted in no response to the elicitor even with coexpression of the functional N protein. Our data provide the first evidence for important roles of introns of a resistance gene in elicitor-responsive gene regulation and efficient defense induction.

    DOI

  • Transient expression of tobacco BBF1-related Dof proteins, BBF2 and BBF3, upregulates genes involved in virus resistance and pathogen defense

    Nobumitsu Sasaki, Masamichi Matsumaru, Shota Odaira, Atsumi Nakata, Keiko Nakata, Ippei Nakayama, Koya Yamaguchi, Hiroshi Nyunoya

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   89   70 - 77  2015年01月  [査読有り]

     概要を見る

    Dof proteins are known as plant specific transcription factors involved in many physiological processes. Although tobacco species are likely to have multiple Dof proteins ( termed as BBF), only BBF1 has been characterized. In this study, we cloned cDNAs of BBF2 and BBF3, which were assumed to be BBF1 homologues, and investigated biochemical and biological functions of BBF2 and BBF3. In gel shift assays, similarly to BBF1, recombinant BBF2 and BBF3 were shown to bind preferentially to DNA with a TAAAG Dof- binding core sequence. Fluorescently labeled BBF2 and BBF3, which were expressed transiently in tobacco epidermal cells, were shown to localize at the nucleus. In addition, as a result of quantitative gene expression analysis, we found that transient expression of each of the three BBF proteins enhanced the expression levels of the virus resistance gene N and multiple defense related genes. Based on these results, we discuss the possibility that BBF2 and BBF3 as well as BBF1 may play roles as transcription factors in the regulation of genes involved in defense responses. (C) 2015 Elsevier Ltd. All rights reserved.

    DOI

  • Formation and intracellular movement of cytoplasmic bodies of the tomato mosaic virus 126-kDa replication protein in association with the viral movement protein

    Nobumitsu Sasaki, Ryuki Shishikura, Hiroshi Nyunoya

    JOURNAL OF GENERAL PLANT PATHOLOGY   80 ( 3 ) 272 - 281  2014年05月  [査読有り]

     概要を見る

    During plant infection by tobamoviruses such as Tomato mosaic virus (ToMV), viral replication complexes containing a viral 126-kDa replicase component (126K) are generated, temporarily in association with a viral movement protein (MP) that is indispensable for viral intercellular movement. The tobamovirus 126K protein has been shown to play an important role not only in replication but also in intercellular transport of viral RNAs. Transient expression of the 126K protein has recently been reported to result in formation of cytoplasmic bodies with shapes similar to those of the replication complexes. In this study, to investigate the roles of ToMV MP in the formation, localization, and dynamics of cytoplasmic bodies of the 126K protein, we carried out confocal microscopy studies of fluorescently tagged 126K and MP that were transiently and simultaneously expressed in Nicotiana benthamiana. Without MP expression, 126K was evenly distributed in the cytoplasm, with occasional formation of small 126K bodies. These small 126K bodies moved in the cytoplasmic stream and along the endoplasmic reticulum network. In contrast, co-expression of MP markedly promoted the formation of larger MP-associated 126K bodies, which were generally static but moved occasionally in the cytoplasmic stream. In addition, we found that small, mobile 126K bodies were captured at the terminal part of MP-derived filamentous structures, leading to the formation of 126K-MP complexes and their translocation to another cellular site.

    DOI

  • Recent progress in research on cell-to-cell movement of rice viruses

    Akihiro Hiraguri, Osamu Netsu, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    FRONTIERS IN MICROBIOLOGY   5   210  2014年05月  [査読有り]

     概要を見る

    To adapt to plants as hosts, plant viruses have evolutionally needed the capacity to modify the host plasmodesmata (PD) that connect adjacent cells. Plant viruses have acquired one or more genes that encode movement proteins (MPs), which facilitate the cell-to-cell movement of infectious virus entities through PD to adjacent cells. Because of the diversity in their genome organization and in their coding sequences, rice viruses may each have a distinct cell-to-cell movement strategy. The complexity of their unusual genome organizations and replication strategies has so far hampered reverse genetic research on their genome in efforts to investigate virally encoded proteins that are involved in viral movement. However, the MP of a particular virus can complement defects in cell-to-cell movement of other distantly related or even unrelated viruses. Trans-complementation experiments using a combination of a movement defective virus and viral proteins of interest to identify MPs of several rice viruses have recently been successful. In this article, we reviewed recent research that has advanced our understanding of cell-to-cell movement of rice viruses.

    DOI PubMed

  • The splice variant Ntr encoded by the tobacco resistance gene N has a role for negative regulation of antiviral defense responses

    Nobumitsu Sasaki, Masumi Takaoka, Shobu Sasaki, Katsuyuki Hirai, Tetsuo Meshi, Hiroshi Nyunoya

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   84   92 - 98  2013年10月  [査読有り]

     概要を見る

    Tobamovirus infection or transient elicitor expression induces a hypersensitive response (HR) in tobacco plants carrying the N resistance gene, which produces two gene products (N and Ntr) through alternative splicing. In this study, the biological significance of Ntr was investigated. The transient overexpression of Ntr markedly inhibited the induction of HR and N-dependent antiviral defense responses in tobacco and Nicotiana benthamiana, respectively. In addition, there were no drastic changes observed in the ratio of the two alternative transcripts during early tobamovirus infection. Our data suggest that Ntr may play a role as a negative regulator of N-dependent antiviral defenses. (C) 2013 Elsevier Ltd. All rights reserved.

    DOI

  • Identification of a movement protein of Mirafiori lettuce big-vein ophiovirus

    Akihiro Hiraguri, Shoko Ueki, Hideki Kondo, Koji Nomiyama, Takumi Shimizu, Tamaki Ichiki-Uehara, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    Journal of General Virology   94 ( 5 ) 1145 - 1150  2013年05月  [査読有り]

     概要を見る

    Mirafiori lettuce big-vein virus (MiLBVV) is a member of the genus Ophiovirus, which is a segmented negative-stranded RNA virus. In microprojectile bombardment experiments to identify a movement protein (MP) gene of ophioviruses that can trans-complement intercellular movement of an MP-deficient heterologous virus, a plasmid containing an infectious clone of a tomato mosaic virus (ToMV) derivative expressing the GFP was co-bombarded with plasmids containing one of three genes from MiLBVV RNAs 1, 2 and 4 onto Nicotiana benthamiana. Intercellular movement of the movement-defective ToMV was restored by co-expression of the 55 kDa protein gene, but not with the two other genes. Transient expression in epidermal cells of N. benthamiana and onion showed that the 55 kDa protein with GFP was localized on the plasmodesmata. The 55 kDa protein encoded in the MiLBVV RNA2 can function as an MP of the virus. This report is the first to describe an ophiovirus MP. © 2013 SGM.

    DOI PubMed

  • Carbonyl sulfide hydrolase from Thiobacillus thioparus strain THI115 is one of the β-carbonic anhydrase family enzymes.

    Ogawa T, Noguchi K, Saito M, Nagahata Y, Kato H, Ohtaki A, Nakayama H, Dohmae N, Matsushita Y, Odaka M, Yohda M, Nyunoya H, Katayama Y

    Journal of the American Chemical Society   135 ( 10 ) 3818 - 3825  2013年03月  [査読有り]

    DOI PubMed

  • Overexpression of a tobacco Dof transcription factor BBF1 stimulates the transcription of the tobacco mosaic virus resistance gene N and defense-related responses including ROS production

    Mayumi Takano, Md. Ashraful Haque, Shota Odaira, Keiko Nakata, Nobumitsu Sasaki, Hiroshi Nyunoya

    PLANT BIOTECHNOLOGY   30 ( 1 ) 37 - U143  2013年  [査読有り]

     概要を見る

    Dof proteins are known as plant-specific transcription factors. We previously reported that Dof proteins might be involved in expression of the tobacco resistance gene N for Tobacco mosaic virus (TMV). In N-carrying tobacco cultivars such as Samsun NN, a rapid upregulation of N transcription is induced by TMV infection, which is followed by the defense response called the hypersensitive response (HR). In this study, the role of a tobacco Dof protein BBF1 in N transcription was investigated. We cloned a BBF1 ORF cDNA of Samsun NN and confirmed that a full-length recombinant BBF1 could bind in vitro to DNA with a Dof binding core motif. The transient overexpression of BBF1 alone did not induce any HR but activated the endogenous N gene expression in Samsun NN. The N promoter activation by BBF1 overexpression was also confirmed in the N-lacking Samsun nn plant by using the exogenously introduced N regulatory sequence connected to a reporter gene. Additional experiments suggested that BBF1 overexpression enhanced not only ROS production but also the transcription activity of certain defense signaling and HR marker genes even without HR induction. Regarding subcellular localization, BBF1 fused with a fluorescent protein was predominantly localized in the nucleus. Based on these data, we discuss potential roles of BBF1 and other Dof proteins as transcription factors for defense responses.

    DOI

  • The movement protein encoded by gene 3 of rice transitory yellowing virus is associated with virus particles

    Akihiro Hiraguri, Hiroyuki Hibino, Takaharu Hayashi, Osamu Netsu, Takumi Shimizu, Tamaki Uehara-Ichiki, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    JOURNAL OF GENERAL VIROLOGY   93 ( Pt 10 ) 2290 - 2298  2012年10月  [査読有り]

     概要を見る

    Gene 3 in the genomes of several plant-infecting rhabdoviruses, including rice transitory yellowing virus (RTYV), has been postulated to encode a cell-to-cell movement protein (MP). Trans-complementation experiments using a movement-defective tomato mosaic virus and the P3 protein of RTYV, encoded by gene 3, facilitated intercellular transport of the mutant virus. In transient-expression experiments with the GFP-fused P3 protein in epidermal leaf cells of Nicotiana benthamiana, the P3 protein was associated with the nucleus and plasmodesmata. Immunogold-labelling studies of thin sections of RTYV-infected rice plants using an antiserum against Escherichia coli-expressed His(6)-tagged P3 protein indicated that the P3 protein was located in cell walls and on virus particles. In Western blots using antisera against E. coli-expressed P3 protein and purified RTYV, the P3 protein was detected in purified RTYV, whilst antiserum against purified RTYV reacted with the E. coli-expressed P3 protein. After immunogold labelling of crude sap from RTYV-infected rice leaves, the P3 protein, as well as the N protein, was detected on the ribonucleocapsid core that emerged from partially disrupted virus particles. These results provide evidence that the P3 protein of RTYV, which functions as a viral MP, is a viral structural protein and seems to be associated with the ribonucleocapsid core of virus particles.

    DOI PubMed

  • Interference with initial and short-distance cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants with high expression of BcKELP, a virus movement protein interactor from Brassica campestris

    Nobumitsu Sasaki, Tatsuro Odawara, Shoko Nagai, Kazuma Yoshimura, Yasuhiko Matsushita, Hiroshi Nyunoya

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   78   38 - 44  2012年04月  [査読有り]

     概要を見る

    A putative transcriptional coactivator KELP of Arabidopsis thaliana and its homolog of Brassica campestris (BcKELP) can bind the movement protein (MP) of Tomato mosaic virus (ToMV) in vitro and, when transiently over-expressed, interfere with the cell-to-cell movement of ToMV. In this study, we generated and selected transgenic Nicotiana tabacum lines expressing BcKELP constitutively and examined their resistance to ToMV. We also investigated changes in MP localization in initially infected Nicotiana benthamiana cells expressing BcKELP transiently. Our results indicate that a high expression of BcKELP can reduce viral initial and short-distance movement probably through disturbance of plasmodesmal targeting of MP. (C) 2012 Elsevier Ltd. All rights reserved.

    DOI

  • Determination of Structural Regions Important for Ca2+ Uptake Activity in Arabidopsis MCA1 and MCA2 Expressed in Yeast

    Masataka Nakano, Kazuko Iida, Hiroshi Nyunoya, Hidetoshi Iida

    PLANT AND CELL PHYSIOLOGY   52 ( 11 ) 1915 - 1930  2011年11月  [査読有り]

     概要を見る

    MCA1 is a plasma membrane protein that correlates Ca2+ influx and mechanosensing in Arabidopsis. MCA2 is a paralog of MCA1, and both share 72.7% amino acid sequence identity and several common structural features, including putative transmembrane (TM) segments, an EF hand-like region in the N-terminal half, a coiled-coil motif in the middle and a PLAC8 motif in the C-terminal half. To determine structural regions important for Ca2+ uptake activity, the activity of truncated forms of MCA1 and MCA2 was assessed using yeast expression assays. The N-terminal half of MCA1 with a coiled-coil motif (MCA1(1-237)) did not have Ca2+ uptake activity, while MCA2(1-237) did. The N-terminal half of MCA1 without the coiled-coil motif (MCA1(1-185)) showed Ca2+ uptake activity, as did MCA2(1-186). Both MCA1(1-173) and MCA2(1-173) having the EF hand-like region had Ca2+ uptake activity. Deletion of a putative TM segment (Ile11-Ala33) and the Asp21 to asparagine mutation in MCA1 and MCA2 abolished Ca2+ uptake activity. Finally, MCA1(173-421) and MCA2(173-416) lacking the N-terminal half had no Ca2+ uptake activity. These results suggest that the N-terminal half of both proteins with the EF hand-like region is necessary and sufficient for Ca2+ uptake and that the coiled-coil motif regulates MCA1 negatively and MCA2 positively.

    DOI PubMed

  • The nonstructural protein pC6 of rice grassy stunt virus trans-complements the cell-to-cell spread of a movement-defective tomato mosaic virus

    Akihiro Hiraguri, Osamu Netsu, Takumi Shimizu, Tamaki Uehara-Ichiki, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    ARCHIVES OF VIROLOGY   156 ( 5 ) 911 - 916  2011年05月  [査読有り]

     概要を見る

    The nonstructural protein pC6 encoded by rice grassy stunt virus is thought to correspond functionally to the nonstructural protein pC4 of rice stripe virus, which can support viral cell-to-cell movement. In a trans-complementation experiment with a movement-defective tomato mosaic virus, pC6 and pC4 facilitated intercellular transport of the virus. Transient expression of pC6, fused with green fluorescent protein, in epidermal cells was predominantly observed close to the cell wall as well as in a few punctate structures, presumably associated with plasmodesmata. These results suggest that pC6 has a role similar to that of pC4 in viral cell-to-cell movement.

    DOI PubMed

  • Characterization and cloning of TMV resistance gene N homologues from Nicotiana tabacum

    Jun-Shan Gao, Yan Meng, Nobumitsu Sasaki, Hiromi Kanegae, Naomi Hayashi, Hiroshi Nyunoya

    AFRICAN JOURNAL OF BIOTECHNOLOGY   9 ( 47 ) 7998 - 8006  2010年11月  [査読有り]

     概要を見る

    Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.

  • Interference with cell-to-cell movement of Tomato mosaic virus by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species

    Nobumitsu Sasaki, Tatsuro Odawara, Masakazu Deguchi, Yasuhiko Matsushita, Hiroshi Nyunoya

    JOURNAL OF GENERAL PLANT PATHOLOGY   76 ( 1 ) 69 - 73  2010年02月

     概要を見る

    The KELP protein of Arabidopsis thaliana (AtKELP), which binds to the Tomato mosaic virus (ToMV) movement protein in vitro, can interfere with cell-to-cell movement of the virus under transient overexpression conditions. In this study, we constructed expression plasmids encoding a fluorescent protein fused to AtKELP or to its homolog from Brassica, Nicotiana, Solanum, or Oryza species. When expressed transiently, all the AtKELP homologs inhibited the cell-to-cell movement of ToMV with different efficiencies. The results of domain swapping between two selected AtKELP homologs suggest the importance of combination between the N- and C-terminal half regions for high inhibitory function.

    DOI CiNii

  • Over-expression of putative transcriptional coactivator KELP interferes with Tomato mosaic virus cell-to-cell movement

    Nobumitsu Sasaki, Takuya Ogata, Masakazu Deguchi, Shoko Nagai, Atsushi Tamai, Tetsuo Meshi, Shigeki Kawakami, Yuichiro Watanabe, Yasuhiko Matsushita, Hiroshi Nyunoya

    MOLECULAR PLANT PATHOLOGY   10 ( 2 ) 161 - 173  2009年03月

     概要を見る

    Tomato mosaic virus (ToMV) encodes a movement protein (MP) that is necessary for virus cell-to-cell movement. We have demonstrated previously that KELP, a putative transcriptional coactivator of Arabidopsis thaliana, and its orthologue from Brassica campestris can bind to ToMV MP in vitro. In this study, we examined the effects of the transient over-expression of KELP on ToMV infection and the intracellular localization of MP in Nicotiana benthamiana, an experimental host of the virus. In co-bombardment experiments, the over-expression of KELP inhibited virus cell-to-cell movement. The N-terminal half of KELP (KELPdC), which had been shown to bind to MP, was sufficient for inhibition. Furthermore, the over-expression of KELP and KELPdC, both of which were co-localized with ToMV MP, led to a reduction in the plasmodesmal association of MP. In the absence of MP expression, KELP was localized in the nucleus and the cytoplasm by the localization signal in its N-terminal half. It was also shown that ToMV amplified normally in protoplasts prepared from leaf tissue that expressed KELP transiently. These results indicate that over-expressed KELP interacts with MP in vivo and exerts an inhibitory effect on MP function for virus cell-to-cell movement, but not on virus amplification in individual cells.

    DOI

  • Identification of a Tobacco mosaic virus elicitor-responsive sequence in the resistance gene N

    Md. Ashraful Haque, Nobumitsu Sasaki, Hiromi Kanegae, Seisuke Mimori, Jun-Shan Gao, Hiroshi Nyunoya

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   73 ( 4-5 ) 101 - 108  2008年11月

     概要を見る

    The N gene of Nicotiana tabacum cv. Samsun NN is a resistance gene for Tobacco mosaic virus. The transcription of the gene increases immediately after virus infection or transient expression of the elicitor p50, a helicase domain of the virus 126/180 K replicases. In this study, we cloned the upstream sequences of the N gene from Samsun NN and fused them to the GFP reporter gene for promoter assays. Agrobacterium-mediated transient gene expression in N. tabacum cv. Samsun nn lacking the N gene allowed us to evaluate promoter activity with or without the expression of p50 and/or N. Individual expression of p50 or N specifically stimulated the N promoter, although the stimulation by N was less prominent than that of p50. The coexpression of p50 and N resulted in higher stimulation of the N promoter than the individual expression. Through deletion and gain-of-function analyses of the upstream region, we identified a 20-bp elicitor-responsive sequence that was essential and sufficient for promoter stimulation by p50 and N. Based on these data, we discuss the possibility that the virus elicitor and N may mediate cooperatively the stimulation of the N promoter upon virus infection. (C) 2009 Elsevier Ltd. All rights reserved.

    DOI

  • The TIR-NBS but not LRR domains of two novel N-like proteins are functionally competent to induce the elicitor p50-dependent hypersensitive response

    Jun-Shan Gao, Nobumitsu Sasaki, Hiromi Kanegae, Ken-ichi Konagaya, Kaori Takizawa, Naomi Hayashi, Yosuke Okano, Masahiro Kasahara, Yasuhiko Matsushita, Hiroshi Nyunoya

    PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY   71 ( 1-3 ) 78 - 87  2007年07月

     概要を見る

    The tobacco N protein recognizes the helicase domain (p50) of the Tobacco mosaic virus (TMV) replicase as an elicitor and mediates hypersensitive response (HR). We obtained two cDNA clones encoding novel N-like (NL) proteins NL-C26 and NL-B69 from Nicotiana tabacum cv. Samsun NN. NL-C26 and NL-B69 had a Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR-NBS-LRR) structure and showed 78% and 73% identities to N, respectively. The NL-C26 and NL-B69 genes were also expressed in N. tabacum cv. Samsun nn, which lacks the N gene. Unlike N, NL-C26 and NL-B69, when coexpressed with p50, failed to induce HR on the sites of agroinfiltration in Samsun nn leaves. However, the elicitor-dependent HR in Samsun nn was induced efficiently by chimeric N proteins with the continuous TIR-NBS domains of NL-C26 and NL-B69. On the other hand, the efficiency of HR induction varied significantly among chimeric N proteins with either of the TIR and NBS domains of the NL proteins. In contrast, chimeras carrying the LRR domains of the NL proteins did not induce HR. Thus, the TIR-NBS domains of NL-C26 and NL-B69 could functionally adapt to the LRR domain of N, which may determine the specificity for the elicitor. We speculate that the NL genes are potential HR-inducing resistance genes for undetermined pathogens other than TMV. (C) 2007 Published by Elsevier Ltd.

    DOI

  • Functional expression of thiocyanate hydrolase is promoted by its activator protein, P15K

    Shingo Kataoka, Takatoshi Arakawa, Shota Hori, Yoko Katayama, Yoshiko Hara, Yasuhiko Matsushita, Hiroshi Nakayama, Masafumi Yohda, Hiroshi Nyunoya, Naoshi Dohmae, Mizuo Maeda, Masafumi Odaka

    FEBS LETTERS   580 ( 19 ) 4667 - 4672  2006年08月

     概要を見る

    Thiocyanate hydrolase (SCNase) is a cobalt-containing enzyme with a post-translationally modified cysteine ligand, gamma Cys131-SO2H. When the SCNase alpha, beta and gamma subunits were expressed in Escherichia coli, the subunits assembled to form a hetero-dodecamer, (alpha beta gamma)(4), like native SCNase but exhibited no catalytic activity. Metal analysis indicated that SCNase was expressed as an apo-form irrespective of the presence of cobalt in the medium. On the contrary, SCNase co-expressed with P15K, encoded just downstream of SCNase genes, in cobalt-enriched medium under the optimized condition (SCNase((+Pi5K))) possessed 0.86 Co atom/alpha beta gamma trimer and exhibited 78% of the activity of native SCNase. SCNase((+Pi5K)) showed a UV-Vis absorption peak characteristic of the SCNase cobalt center. About 70% of SCNase((+P15K)) had the gamma Cys131-SO2H modification. These results indicate that SCNase((+P15K)) is the active holo-SCNase. P15K is likely to promote the functional expression of SCNase probably by assisting the incorporation of cobalt ion. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI PubMed CiNii

  • Thiocyanate hydrolase is a cobalt-containing metalloenzyme with a cysteine-sulfinic acid ligand

    Y Katayama, K Hashimoto, H Nakayama, H Mino, M Nojiri, T Ono, H Nyunoya, M Yohda, K Takio, M Odaka

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   128 ( 3 ) 728 - 729  2006年01月

    DOI PubMed CiNii

  • Identification of estrogenic compounds in wastewater effluent

    N Nakada, H Nyunoya, M Nakamura, A Hara, T Iguchi, H Takada

    ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY   23 ( 12 ) 2807 - 2815  2004年12月

     概要を見る

    In order to identify the dominant contributors to estrogenic activity in environmental waters, a comprehensive fractionation method using silica gel column chromatography, combined with recombinant yeast assay for detecting estrogenic activity and with gas chromatography-mass spectrometry for quantifying endocrine disruptors and natural estrogens, was developed. The method was applied to the municipal sewage treatment plant (STP) secondary effluent discharged to the Tamagawa River in Tokyo, Japan, where endocrine disruption was observed in wild carp. The instrumental analysis demonstrated that averaged concentrations of nonylphenol, bisphenol A, estrone (El), and 17beta-estradiol (E2) were 564 +/- 127, 27 +/- 19, 33 +/- 11, and 4.6 +/- 3.0 ng/L, respectively. Based on the concentration and relative potency of these compounds, the natural estrogens El and E2 represented more than 98% of the total estrogen equivalent concentration (EEQ) in the STP effluent, while the contribution of phenolic compounds to total EEQ was less than 2%. Estrogenic activities associated with the dissolved phase of the effluent samples were detected by a recombinant yeast assay. By using silica gel column chromatography, the dissolved phase was separated into several fractions that were subjected to the bioassay. The polar fractions exhibited estrogenic activity. The greatest estrogenic activity was found in a polar fraction containing E1 and E2 and represented 66 to 88% of the total estrogenic activities estimated from the bioassay data. These results lead to the conclusion that E1 and E2 were the dominant environmental estrogens in the STP effluent, but a significant contribution to estrogenic activities stems from unidentified components in the effluents.

    DOI PubMed CiNii

  • Expression of gibberellin biosynthetic gene in Japanese weeping cherry.

    Sugano M, Nyunoya H, Nakamura T

    Uchu Seibutsu Kagaku   18 ( 3 ) 184 - 185  2004年11月  [査読有り]

    PubMed

  • Brassinosteroid deficiency due to truncated steroid 5 alpha-reductase causes dwarfism in the lk mutant of pea

    T Nomura, CE Jager, Y Kitasaka, K Takeuchi, M Fukami, K Yoneyama, Y Matsushita, H Nyunoya, S Takatsuto, S Fujioka, JJ Smith, LHJ Kerckhoffs, JB Reid, T Yokota

    PLANT PHYSIOLOGY   135 ( 4 ) 2220 - 2229  2004年08月

     概要を見る

    The endogenous brassinosteroids in the dwarf mutant lk of pea (Pisum sativum) were quantified by gas chromatography-selected ion monitoring. The levels of castasterone, 6-deoxocastasterone, and 6-deoxotyphasterol in lk shoots were reduced 4-, 70-, and 6-fold, respectively, compared with those of the wild type. The fact that the application of brassinolide restored the growth of the mutant indicated that the dwarf mutant lk is brassinosteroid deficient. Gas chromatography-selected ion monitoring analysis of the endogenous sterols in lk shoots revealed that the levels of campestanol and sitostanol were reduced 160- and 10-fold, respectively, compared with those of wild-type plants. These data, along with metabolic studies, showed that the lk mutant has a defect in the conversion of campest-4-en-3-one to 5alpha-campestan-3-one, which is a key hydrogenation step in the synthesis of campestanol from campesterol. This defect is the same as that found in the Arabidopsis det2 mutant and the Ipomoea nil kbt mutant. The pea gene homologous to the DET2 gene, PsDET2, was cloned, and it was found that the lk mutation would result in a putative truncated PsDET2 protein. Thus it was concluded that the short stature of the lk mutant is due to a defect in the steroidal 5alpha-reductase gene. This defect was also observed in the callus induced from the lk mutant. Biosynthetic pathways involved in the conversion of campesterol to campestanol are discussed in detail.

    DOI PubMed CiNii

  • Interaction of Tomato mosaic virus movement protein with tobacco RIO kinase

    K Yoshioka, Y Matsushita, M Kasahara, K Konagaya, H Nyunoya

    MOLECULES AND CELLS   17 ( 2 ) 223 - 229  2004年04月

     概要を見る

    Tomato mosaic virus (ToMV) has a regulatory gene encoding a movement protein (MP) that is involved in the cell-to-cell movement of viral RNA through plasmodesmata. To identify the host cell factors interacting with ToMV MP, we used a recombinant NIP probe to isolate cDNA clones from a phage expression library of Nicotiana tabacum by a far-Western screening method. One of the cDNA clones encoded an MP-interacting protein, MIP-T7, homologous to the yeast novel protein kinase, Rio1p. We isolated a full-length cDNA by RT-PCR. The putative gene product was designated NtRIO, and shared 33 and 73% amino acid identity with yeast and Arabidopsis RIO kinases, respectively. In vitro analyses using recombinant proteins showed that NtRIO also interacted with a different MP derived from Cucumber mosaic virus. NtRIO had autophosphorylation activity and phosphorylated ToMV ME Addition of recombinant tobacco casein kinase 2 resulted in a marked increase in the phosphorylation of NtRIO. The interaction between NtRIO and ToMV NIP was inhibited by phosphorylation of NtRIO.

  • Cytokinin response in root of Arabidopsis thaliana overexpressed CBP2 homolog (AtOLP)

    K Katashima, K Okada, Y Matushita, H Nyunoya, K Kobayashi

    PLANT AND CELL PHYSIOLOGY   45   S40 - S40  2004年

  • Expression of gibberellin 3β-hydroxylase gene in a gravi-response mutant, weeping Japanese flowering cherry

    M. Sugano, Y. Nakagawa, H. Nyunoya, T. Nakamura

    Biological Sciences in Space   18 ( 4 ) 261 - 266  2004年

    DOI PubMed CiNii

  • Two proton pump interactors identified from a direct phosphorylation screening of a rice cDNA library by using a recombinant BRI1 receptor kinase

    Shuichi Hirabayashi, Yasuhiko Matsushita, Michio Sato, Rie Oh-I, Masahiro Kasahara, Hiroshi Abe, Hiroshi Nyunoya

    Plant Biotechnology   21 ( 1 ) 35 - 45  2004年

     概要を見る

    A direct phosphorylation screening of a rice cDNA library resulted in isolation of 35 BIP clones encoding brassinosteroid receptor kinase (BRI1)-interacting proteins. Among the candidate substrates for BRI1, two clones were found to encode similar proton pump interactor proteins homologous to Arabidopsis PPI1, which was reported to interact with a regulatory region of plasma membrane H+-ATPase. The rice proton pump interactors BIP103 and BIP131 contained 627 and 621 amino acids, respectively, with carboxyl-terminal hydrophobic region characteristic of tail-anchored proteins. Northern blotting analysis indicated that mRNAs for both interactors increased significantly after brassinolide treatment of lamina joint cells, which are especially sensitive to exogenous brassinosteroids.

    DOI CiNii

  • Members of 14-3-3 protein isoforms interacting with the resistance gene product N and the elicitor of Tobacco mosaic virus

    Ken-Ichi Konagaya, Yasuhiko Matsushita, Masahiro Kasahara, Hiroshi Nyunoya

    Journal of General Plant Pathology   70 ( 4 ) 221 - 231  2004年

     概要を見る

    A functional cDNA for the resistance gene N from Nicotiana tabacum cv. Samsun NN to Tobacco mosaic virus (TMV) was obtained by reverse transcription polymerase chain reaction. Recombinant proteins containing various domains of the N protein were used in far-Western screening for the N-interacting protein from tobacco cDNA expression libraries. Nucleotide sequence analysis revealed that 199 cDNAs of 217 positive clones screened from 1.6 × 106 phage plaques were related to the 14-3-3 gene family. Phylogenetic analysis indicated that the 14-3-3 proteins were divided into 17 different 14-3-3 isoforms, including hitherto unidentified novel isoforms i-1 and i-2. In vitro binding assays showed that the probes TIR and LRR domains of N protein and helicase (HEL) domain of TMV replicase bound significantly to 14-3-3 isoforms of groups ω and ψ. These domains barely bound to those of group κ and did not bind to that of group ε. The same probes did not bind to any domains of N protein or the viral HEL domain. Northern blotting showed an increase in 14-3-3 isoform h transcripts coinciding with PR-1a induction after TMV inoculation. The 14-3-3 isoform h as a probe had significant binding to the LRR domain of N protein and the viral HEL domain, but there was only minor binding to the TIR domain. These results raise the possibility that 14-3-3 acts as a scaffolding or adaptor between the N protein and the helicase domain to support the interaction between the R gene product and the viral elicitor.

    DOI

  • The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus

    Y Matsushita, M Ohshima, K Yoshioka, M Nishiguchi, H Nyunoya

    JOURNAL OF GENERAL VIROLOGY   84 ( 2 ) 497 - 505  2003年02月

     概要を見る

    The movement protein (MP) of Tomato mosaic virus (ToMV) was reported previously by us to be phosphorylated in vitro by a cellular protein kinase(s) that exhibited several characteristics of casein kinase 2 (CK2). To characterize further this CK2-like cellular kinase, we have cloned cDNAs encoding the CK2 catalytic subunit from tobacco and compared the properties of the recombinant CK2-like cellular kinase. The recombinant CK2 catalytic subunit formed a protein with those of the CK2 complex with ToMV MP and phosphorylated it, similar to the CK2-like cellular kinase.
    Phosphoamino acid analyses of various mutant MPs altered near the C terminus revealed that the recombinant CK2 catalytic subunit phosphorylated serine-261, while the CK2-like cellular kinase phosphorylated both serine-261 and threonine-256. Both kinases were suggested to phosphorylate an additional serine residue(s) in regions other than the C-terminal peptide. The results are consistent with our previous prediction of involvement of CK2 in phosphorylation of ToMV MP.

    DOI PubMed CiNii

  • Cloning of a tobacco cDNA coding for a putative transcriptional coactivator MBF1 that interacts with the tomato mosaic virus movement protein

    Y Matsushita, O Miyakawa, M Deguchi, M Nishiguchi, H Nyunoya

    JOURNAL OF EXPERIMENTAL BOTANY   53 ( 373 ) 1531 - 1532  2002年06月

     概要を見る

    Viral movement through plasmodesmata in host plants depends on the interaction between virus-encoded movement protein (MP) and host proteins. To search for MP-interacting protein (MIP), far-western screening of a tobacco cDNA library was carried out using a recombinant MP of tomato mosaic virus (ToMV) as a probe. One of the positive cDNA clones, designated MIP204, was highly homologous to a class of transcriptional coactivators commonly referred to as multiprotein bridging factor 1 (MBF1). ToMV MP could also bind to the Arabidopsis homologues of MBF1. The recombinant MIP204 bound to MPs of ToMV and a crucifer tobamovirus CTMV-W, but not of cucumber mosaic virus. MPs of ToMV and the related virus may interact with MBF1-like proteins to modulate host gene expression.

    DOI PubMed CiNii

  • Phosphorylation of the movement protein of Cucumber mosaic virus in transgenic tobacco plants

    Y Matsushita, K Yoshioka, T Shigyo, H Takahashi, H Nyunoya

    VIRUS GENES   24 ( 3 ) 231 - 234  2002年06月

     概要を見る

    The 3a protein of Cucumber mosaic virus is essential for the cell-to-cell movement of the viral RNA through plasmodesmata. We have introduced an epitope peptide before the stop codon of the 3a protein and cloned the tagged ORF into a binary vector for Agrobacterium-mediated transformation. The established transgenic tobacco lines produced the 3a protein, which was specifically detected with anti-3a and anti-epitope antisera. Metabolic labeling and subsequent immunoprecipitation revealed that [P-32]-orthophosphate was incorporated into the 3a protein. The phosphoamino acid analysis indicated that the 3a protein contained phosphoserine but not phosphothreonine or phosphotyrosine. This is the first demonstration of the 3a protein phosphorylation in planta.

    DOI

  • Genetic and immunochemical characterization of thiocyanate-degrading bacteria in lake water (vol 68, pg 942, 2002)

    M Yamasaki, Y Matsushita, M Namura, H Nyunoya, Y Katayama

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   68 ( 5 ) 2632 - 2632  2002年05月

    DOI

  • Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

    Y Matsushita, T Suzuki, R Kubota, M Mori, H Shimosato, M Watanabe, T Kayano, T Nishio, H Nyunoya

    JOURNAL OF EXPERIMENTAL BOTANY   53 ( 369 ) 765 - 767  2002年04月  [査読有り]

     概要を見る

    SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

    DOI PubMed

  • Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

    Y Matsushita, T Suzuki, R Kubota, M Mori, H Shimosato, M Watanabe, T Kayano, T Nishio, H Nyunoya

    JOURNAL OF EXPERIMENTAL BOTANY   53 ( 369 ) 765 - 767  2002年04月

     概要を見る

    SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

    DOI

  • Genetic and immunochemical characterization of thiocyanate-degrading bacteria in lake water

    M Yamasaki, Y Matsushita, M Namura, H Nyunoya, Y Katayama

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   68 ( 2 ) 942 - 946  2002年02月  [査読有り]

     概要を見る

    Natural aquatic and soil samples were screened for the presence of thiocyanate-degrading bacteria. Using thiocyanate supplementation, we established an enrichment culture containing such bacteria from lake water. The dominant bacteria had the scnC-LS5 gene encoding thiocyanate hydrolase, which was closely related to the enzyme found previously in Thiobacillus thioparus THI115 isolated from activated sludge.

  • Effect of light quality on the level of endogenous brassinosteroids in rice, and the rice DWARF gene

    Y Tamaki, K Takeuchi, T Nomura, K Yoneyama, Y Takeuchi, H Nyunoya, Y Matsushita, S Takatsuto, GJ Bishop, T Yokota

    PLANT AND CELL PHYSIOLOGY   43   S185 - S185  2002年

  • Screening for the intracellular signal transduction factors that interact with the BRI1 kinase domain

    S Hirabayashi, Y Matsushita, M Sato, R Oh-i, H Abe, H Nyunoya

    PLANT AND CELL PHYSIOLOGY   43   S93 - S93  2002年

  • The tomato mosaic tobamovirus movement protein interacts with a putative transcriptional coactivator KELP

    Y Matsushita, M Deguchi, M Youda, M Nishiguchi, H Nyunoya

    MOLECULES AND CELLS   12 ( 1 ) 57 - 66  2001年08月

     概要を見る

    Viral movement through plasmodesmata in host plants likely depends on the interaction between virus-encoded movement protein (MP) and host proteins. In order to search for MP-interacting protein (MIP), we carried out far-western screening of a Brassica campestris cDNA library using a recombinant MP of tomato mosaic tobamovirus (ToMV) as a probe. One of the positive clones, designated MIP102, was found to be a putative orthologue for a transcriptional coactivator KELP of Arabidopsis thaliana. In vitro analysis with recombinant proteins revealed that ToMV MP could bind to KELP proteins that are derived from different plant species. At least 31 amino acids from the carboxyl-terminus of ToMV MP were dispensable for the interaction with KELP. Other MPs, derived from crucifer tobamovirus CTMV-W and cucumber mosaic cucumovirus, also exhibited comparable binding abilities. This suggests that these MPs could commonly interact with KELP, possibly to modulate the host gene expression.

  • The molecular characterization and in situ expression pattern of pea SCARECROW gene

    N Sassa, Y Matsushita, T Nakamura, H Nyunoya

    PLANT AND CELL PHYSIOLOGY   42 ( 4 ) 385 - 394  2001年04月

     概要を見る

    Certain mutants of shoot gravitropism were reported to be ascribed to the SCR and SHR loci in Arabinopsis thaliana, The SCR gene was known to regulate the development of endodermis cells that are responsible for sensing gravity in a shoot. With the aim of elucidating the molecular mechanism for gravitropic responses in pea seedlings, we have isolated a putative pea SCR ortholog from a shoot cDNA library. Analyses of the cDNA clones revealed the structure of a full-length ORF coding for 819 amino acid residues, A remarkable feature of pea SCR protein was the presence of asparagine stretches at the N-terminal transcriptional activation domain, which was distinct from the occurrence of glutamine or alanine stretches in the Arabidopsis or maize SCR, A Northern blot analysis revealed a single 3.2-kb pea SCR transcript in addition to a closely related 2.5-kb transcript, Our in situ hybridization data indicated that pea SCR mRNA accumulated in the shoot apical meristem, leaf primordia and a root single cell layer corresponding to the endodermis. The expression patterns were similar to those reported for A. thaliana and Zea mays, suggesting that SCR may be functionally conserved among plants and involved in the differentiation of the endodermis.

  • In vitro phosphorylation of the movement protein of tomato mosaic tobamovirus by a cellular kinase

    Y Matsushita, K Hanazawa, K Yoshioka, T Oguchi, S Kawakami, Y Watanabe, M Nishiguchi, H Nyunoya

    JOURNAL OF GENERAL VIROLOGY   81   2095 - 2102  2000年08月

     概要を見る

    The movement protein (MP) of tomato mosaic virus (ToMV) was produced in E. coli as a soluble fusion protein with glutathione S-transferase. When immobilized on glutathione affinity beads, the recombinant protein was phosphorylated in vitro by incubating with cell extracts of Nicotiana tabacum and tobacco suspension culture cells (BY-2) in the presence of [gamma-P-32]ATP. Phosphorylation occurred even after washing the beads with a detergent-containing buffer, indicating that the recombinant MP formed a stable complex with some protein kinase(s) during incubation with the cell extract. Phosphoamino acid analysis revealed that the MP was phosphorylated on serine and threonine residues. Phosphorylation of the MP was decreased by addition of kinase inhibitors such as heparin, suramin and quercetin, which are known to be effective for casein kinase II (CK II). The phosphorylation level was not changed by other types of inhibitor. In addition, as shown for animal and plant CK II, [gamma-P-32]GTP was efficiently used as a phosphoryl donor. Phosphorylation was not affected by amino acid replacements at serine-37 and serine-238, but was completely inhibited by deletion of the carboxy-terminal 9 amino acids, including threonine-256, serine-257, serine-261 and serine-263. These results suggest that the MP of ToMV could be phosphorylated in plant cells by a host protein kinase that is closely related to CK II.

  • Thiobacillus thioparus が生産する新規なチオシアネ-ト分解酵素

    片山葉子, 丹生谷博

    バイオサイエンスとインダストリ−   57 ( 6 ) 25 - 28  1999年

  • Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase

    Y Katayama, Y Matsushita, M Kaneko, M Kondo, T Mizuno, H Nyunoya

    JOURNAL OF BACTERIOLOGY   180 ( 10 ) 2583 - 2589  1998年05月

     概要を見る

    Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol, Chem, 267:9170-9175, 1992), We have cloned and sequenced the scn genes that encode the three subunits of the enzyme, The scnB, scnA, and scnC genes, arrayed in this order, contained open reading frames encoding sequences of 157, 126, and 243 amino acid residues, respectively, for the beta, alpha, and gamma subunits, respectively, Each open reading frame was preceded by a typical Shine-Dalgarno sequence. The deduced amino-terminal peptide sequences for the three subunits were in fair agreement with the chemically determined sequences. The protein molecular mass calculated for each subunit was compatible with that determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, From a computer analysis, thiocyanate hydrolase showed significant homologies to bacterial nitrile hydratases known to convert nitrile to the corresponding amide, which is further hydrolyzed by amidase to form acid and ammonia. The two enzymes were homologous over regions corresponding to almost the entire coding regions of the genes: the beta and alpha subunits of thiocyanate hydrolase were homologous to the amino- and carboxyl-terminal halves of the beta subunit of nitrile hydratase, and the gamma subunit of thiocyanate hydrolase was homologous to the alpha subunit of nitrile hydratase, Comparisons of the catalytic properties of the two homologous enzymes support the model for the reaction steps of thiocyanate hydrolase that was previously presented on the basis of biochemical analyses.

  • Characterization of peripheral blood T-lymphocytes transduced with HTLV-I Tax mutants with different trans-activating phenotypes

    T Akagi, H Ono, H Nyunoya, K Shimotohno

    ONCOGENE   14 ( 17 ) 2071 - 2078  1997年05月

     概要を見る

    Tax1, a transcriptional trans-activator of the Human T-cell leukemia virus type I (HTLV-I), induces the expression of many cellular genes through interaction with at least three distinct cellular transcription factors; CREB/ATF, NF-kappa B, and SRF. This Tax1-induced activation of cellular genes is considered to be a critical event in T-cell transformation by HTLV-I. To elucidate the role of each Taxi-inducible transcriptional pathway in T-cell transformation, we introduced Tax1 mutants with different trans-activating phenotypes into peripheral blood lymphocytes (PBL) by retroviral vectors. Analysis of these PBLs revealed that activation of the NF-kappa B pathway is sufficient to promote the growth response to IL-2. However, for the clonal expansion of CD4+ T-cells, which is a characteristic result of HTLV-I infection, activation of the CREB/ATF and SRF pathways is also required.

    DOI

  • Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells

    M Inudoh, H Nyunoya, T Tanaka, M Hijikata, N Kato, K Shimotohno

    VACCINE   14 ( 17-18 ) 1590 - 1596  1996年12月

     概要を見る

    A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence, E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the complex form of E2 protein was greater than that of the high-mannose form of E2 protein. This result indicated that the complex form of the E2 protein is superior for use in diagnosing HCV infection. Copyright (C) 1996 Elsevier Science Ltd.

    DOI

  • Src-homology domain 2 is responsible for transcriptional suppression induced by expression of Lck

    M Ohta, H Nagai, H Nyunoya, K Shimotohno

    ONCOGENE   12 ( 5 ) 989 - 997  1996年03月

     概要を見る

    Overexpression of Lck was shown, by our previous study, to suppress gene transcription from various viral and cellular promoters. The suppression of transcription from human T-cell leukemia virus promoter by Lck was independent of the presence of the enhancer core sequences within the long terminal repeat. The suppression of transcription was observed with Lck mutants that had either diminished or enhanced tyrosine-kinase activity. A mutant lacking the myristylation site also suppressed transcription. From the analysis with various deletion mutants of Lck, it was suggested that Src-homology domain 2 (SH2) is both necessary and sufficient for the suppression of transcription. A similar effect was also observed with the SH2 domain of the v-src gene. Thus, overexpression of Lck could suppress gene expression through a unique function of the SH2 domain.

  • HIGH-INCIDENCE OF HAM/TSP-LIKE SYMPTOMS IN WKA RATS AFTER ADMINISTRATION OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE 1-PRODUCING CELLS

    S KUSHIDA, H MIZUSAWA, M MATSUMURA, H TANAKA, Y AMI, M HORI, KI YAGAMI, T KAMEYAMA, Y TANAKA, A YOSHIDA, H NYUNOYA, K SHIMOTOHNO, Y IWASAKI, K UCHIDA, M MIWA

    JOURNAL OF VIROLOGY   68 ( 11 ) 7221 - 7226  1994年11月

     概要を見る

    We demonstrate a significantly high incidence of human T-cell leukemia virus type 1 (HTLV-1)-associated myelopathy (HAM)- or tropical spastic paraparesis (TSP)-like symptoms in WKA rats after injection with HTLV-1-producing MT-2 cells, while no symptoms were observed in F344 rats injected with MT-2 cells or in control WKA rats. Five of the eight (63%) WKA rats injected with MT-2 cells showed HAM/TSP-like paraparesis at 105 weeks of age, but none of seven MT-2-injected F344 rats or eight control WKA rats showed symptoms. This high incidence of HAM/TSP-like symptoms in WKA rats was statistically significant (P &lt; 0.05). Six of the eight (75%) WKA rats injected with MT-2 cells showed HAM/TSP-like paraparesis at 108 weeks of age. HAM/TSP-like symptoms were also observed in one of the two WKA rats injected with HTLV-1-producing Ra-1 cells at 128 weeks of age. HTLV-1 provirus was detected in peripheral blood mononuclear cells in both WKA and F344 rats, The provirus was detected in the spinal cords of the HAM/TSP-like WKA rats that had severe neuropathological changes. WKA and F344 rats showed no significant difference in antibody response against HTLV-1 Gag antigen. However, the antibody response against the C-terminal half of gp46 HTLV-1 envelope protein was lower in WKA rats than in F344 rats. Pathological analysis of the HAM/TSP-like rats showed degeneration of the white matter of the spinal cord and peripheral nerves. These findings suggest that both the genetic background of the host and HTLV-1 infection are important in neuropathogenesis of HAM/TSP-like paraparesis in rats.

  • CLONING OF A CDNA-ENCODING A DNA-BINDING PROTEIN TAXREB302 THAT IS SPECIFIC FOR THE TAX-RESPONSIVE ENHANCER OF HTLV-I (VOL 126, PG 251, 1993)

    H NYUNOYA, T MORITA, T SATO, S HONMA, A TSUJIMOTO, K SHIMOTOHNO

    GENE   148 ( 2 ) 371 - 373  1994年10月

  • Two proteinase activities in HCV polypeptide expressed in insect cells using baculovirus vector

    Y. Hirowatari, M. Hijikata, Y. Tanji, H. Nyunoya, H. Mizushima, K. Kimura, T. Tanaka, N. Kato, K. Shimotohno

    Archives of Virology   133 ( 3-4 ) 349 - 356  1993年09月

     概要を見る

    Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P 1 and P 1′ of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity. © 1993 Springer-Verlag.

    DOI PubMed

  • CLONING OF A CDNA-ENCODING A DNA-BINDING PROTEIN TAXREB302 THAT IS SPECIFIC FOR THE TAX-RESPONSIVE ENHANCER OF HTLV-I

    H NYUNOYA, T MORITA, T SATO, S HONMA, A TSUJIMOTO, K SHIMOTOHNO

    GENE   126 ( 2 ) 251 - 255  1993年04月

     概要を見る

    The transcriptional activator, Tax, of human T-cell leukemia virus (HTLV-I) has been considered to interact with cellular proteins to act on target enhancer motifs. Using oligodeoxyribonucleotides containing the tax-responsive element (TAXRE) of the HTLV-I enhancer, we have cloned multiple cDNAs coding for TAXRE-binding proteins (TAXREB), and determined the cDNA and the deduced 200-amino-acid sequences for TAXREB302. The recombinant protein binds to the enhancer DNA by specific interaction to the CRE-like sequence. A single 1.8-kb species of mRNA was detected in cultured cells, as well as in normal human tissues, especially brain and skeletal muscle. The 22-kDa native protein was detected in the cultured-cell lysate by immunoblotting analysis. TAXREB302 does not have structural features common to the CRE-binding protein or activating transcription factor (CREB/ATF) family, but has homology to chicken erythroid transcription factor (Eryf1 or GATA-1), suggesting a possible protein-protein interaction.

    DOI

  • ISOLATION OF A CDNA CLONE ENCODING DNA-BINDING PROTEIN (TAXREB107) THAT BINDS SPECIFICALLY TO DOMAIN-C OF THE TAX-RESPONSIVE ENHANCER ELEMENT IN THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I

    T MORITA, T SATO, H NYUNOYA, A TSUJIMOTO, J TAKAHARA, S IRINO, K SHIMOTOHNO

    AIDS RESEARCH AND HUMAN RETROVIRUSES   9 ( 2 ) 115 - 121  1993年02月

     概要を見る

    Five cDNA clones for TAXREB proteins that bind to the tax-responsive enhancer element of human T-cell leukemia virus type I (HTLV-I) were isolated from a Jurkat cell cDNA library. The beta-galactosidase fusion proteins of three of these clones specifically recognized the domain C within the enhancer. One of the three cDNAs, encoding TAXREB107, contained an open reading frame with 288 amino acid residues. RNA blot analysis showed that the level of mRNA for TAXREB107 increased transiently in Jurkat cells on treatment with TPA. Immunoblot analysis showed that polyclonal antibody against TAXREB107 specifically recognized a 34-kD protein in Jurkat cells. TAXREB107 may participate in tax-mediated trans-activation of transcription.

  • 2 PROTEINASE ACTIVITIES IN HCV POLYPEPTIDE EXPRESSED IN INSECT CELLS USING BACULOVIRUS VECTOR

    Y HIROWATARI, M HIJIKATA, Y TANJI, H NYUNOYA, H MIZUSHIMA, K KIMURA, T TANAKA, N KATO, K SHIMOTOHNO

    ARCHIVES OF VIROLOGY   133 ( 3-4 ) 349 - 356  1993年  [査読有り]

     概要を見る

    Processing of HCV viral precursor protein requires at least two viral proteinases, Cpro-1 and Cpro-2, in addition to cellular proteinases. The HCV polypeptide that covers the region for the two viral proteinase domains was expressed in insect cells using a baculovirus expression system. The two proteinase activities were demonstrated in the infected cells. The Cpro-1-dependent cleavage site was estimated from the amino acid sequence of the N-terminus of the processed product. Analyses of the susceptibilities of various mutants altered at position P1 and P1' of the putative cleavage site suggested that amino acid residues at these positions is not essential for recognition and cleavage by Cpro-1-dependent activity.

  • EVALUATION OF ENZYME-IMMUNOASSAY USING A RECOMBINANT ENVELOPE PROTEIN EXPRESSED IN INSECT CELLS FOR SEROLOGICAL CONFIRMATION OF HTLV-I INFECTION

    K YAMASHITA, M MAEKAWA, K MITANI, M WAKAMIYA, T OGINO, K MIYAMURA, K BABA, Y YAMAMOTO, H NYUNOYA, K SHIMOTOHNO, Y TAKEBE, S YAMAZAKI

    AIDS RESEARCH AND HUMAN RETROVIRUSES   8 ( 11 ) 1857 - 1861  1992年11月

     概要を見る

    A recombinant human T-lymphotropic virus type I (HTLV-I) envelope protein expressed in insect cells using a recombinant baculovirus was employed as the antigen in an enzyme immunoassay (renvEIA). Peripheral blood samples were obtained from asymptomatic carriers or healthy individuals. Plasma was tested for HTLV-I antibody by renvEIA, particle agglutination, and Western immunoblot (WB), and lymphocyte DNA was tested for HTLV-I proviral DNA amplification by polymerase chain reaction (PCR). Of 61 people aged 9 months or older, 23 were positive (gag+, env+) and 19 others were in the "indeterminate" category (gag+, env-) when their WB results were interpreted according to the WHO-proposed criteria. Thirty-seven cases, including all of the WB+ cases and 14 of 19 WB indeterminate cases, were positive by renvEIA. In 34 of 37 renvEIA-positive cases, the presence of long terminal repeat (LTR) and tax/rex region of HTLV-I proviral DNA was detected by polymerase chain reaction (PCR) and following Southern blot hybridization. Thus, renvEIA would be a useful supplemental assay to confirm the presence of HTLV-I antibody in HTLV-I asymptomatic carriers.

  • AN ANTIGENIC STRUCTURE OF THE TRANSACTIVATOR PROTEIN ENCODED BY HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I (HTLV-I), AS DEFINED BY A PANEL OF MONOCLONAL-ANTIBODIES

    Y TANAKA, M MASUDA, A YOSHIDA, H SHIDA, H NYUNOYA, K SHIMOTOHNO, H TOZAWA

    AIDS RESEARCH AND HUMAN RETROVIRUSES   8 ( 2 ) 227 - 235  1992年02月

     概要を見る

    In order to study an antigenic structure of the trans-activator protein encoded by human T-cell leukemia virus type-I (HTLV-I), tax1 antigen, we generated and characterized a panel of rat anti-tax1 monoclonal antibodies (MAbs) designated WATM-1, WATM-2, WATM-3, and WATM-4. These MAbs were derived from WKA rats immunized with HTLV-I-transformed (HTLV-I+) syngeneic T cells. Immunoblot assays showed that: (1) All the MAbs reacted with the tax1 antigen in HTLV-I+ cell lines and a recombinant tax1 antigen, PX141 (containing entire tax1 polypeptide); (2) WATM-3 and WATM-4, but not WATM-1 or WATM-2, reacted with a truncated tax1 antigen, XD59 (tax1 amino acids 180-338); (3) None of them reacted with another truncated tax1 antigen, XD128 (tax1 amino acids 1-47 and 286-353); and (4) each of the four MAbs had different reactivity with tax1-related antigens in the range 38-41 kDa expressed in simian cell lines infected with various HTLV-I-related simian retroviruses (STLV-I). None of the MAbs reacted with HTLV-II tax antigen. Human sera containing anti-tax1 antibodies interfered specifically with the antigen-specific binding of all the MAbs. These results suggest that the present rat MAbs are directed against various epitopes on the tax1 antigen. An antigenic structure of the tax1 antigen deduced from reactivity of a panel of anti-tax1 MAbs including the present rat MAbs is discussed.

  • DIFFERENTIAL EXPRESSION OF POLY(ADP-RIBOSE) POLYMERASE AND DNA POLYMERASE-BETA IN RAT-TISSUES

    M MENEGAZZI, G GRASSIZUCCONI, AC DEPRATI, T OGURA, P POLTRONIERI, H NYUNOYA, Y SHIRATORINYUNOYA, M MIWA, H SUZUKI

    EXPERIMENTAL CELL RESEARCH   197 ( 1 ) 66 - 74  1991年11月

    DOI

  • MURINE RETROVIRAL VECTORS EXPRESSING THE TAX1 GENE OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1

    T AKAGI, H NYUNOYA, K SHIMOTOHNO

    GENE   106 ( 2 ) 255 - 259  1991年10月

     概要を見る

    Murine retroviral vectors which express the tax1 gene of human T-cell leukemia virus type 1 (HTLV-1) have been constructed. A significant increase in virus titer achieved by inserting a portion of the gag region of Moloney murine leukemia virus. Using these vectors, tax1 was stably introduced into primary human T-cells derived from peripheral blood lymphocytes. Expression of the functional tax1 in infected cells was confirmed by trans-activation of HTLV-1 long terminal repeat-directed transcription. These vectors provide a useful means of investigating the function of tax1 in natural target cells for HTLV-1.

    DOI

  • PRODUCTION OF A RECOMBINANT HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I TRANSACTIVATOR (TAX1) ANTIGEN AND ITS UTILIZATION FOR GENERATION OF MONOCLONAL-ANTIBODIES AGAINST VARIOUS EPITOPES ON THE TAX1 ANTIGEN

    Y TANAKA, A YOSHIDA, H TOZAWA, H SHIDA, H NYUNOYA, K SHIMOTOHNO

    INTERNATIONAL JOURNAL OF CANCER   48 ( 4 ) 623 - 630  1991年06月

     概要を見る

    A 42-kDa recombinant protein, PX141, consisting of the trans-activator protein encoded by human T-cell leukemia virus (HTLV-I) (tax1 antigen) and the amino-terminal fusion peptide of 12 amino acid residues of the alpha-peptide encoded by the plasmid pUC19 was produced. In order to investigate the immunogenicity of the tax1 antigen, mice were immunized with the purified PX141 and 4 anti-tax1 monoclonal antibodies (MAbs) designated TAXY-1, TAXY-6, TAXY-7 and TAXY-8 were generated, and their reactivity was characterized along with another anti-tax1 MAb, Lt-4. Immunoblot assays showed that all the MAbs reacted with the PX141, the native tax1 antigen expressed in various HTLV-1-infected cell lines and the gp68 of MT-2 cells expressing the tax1 amino acids 94-353. Immunoblot assays using recombinant, truncated tax1 antigens, XD59 (expressing amino acids 180-338) and XD128 (expressing amino acids 1-47 and 286-353) showed that: (1) TAXY-1 and Lt-4 did not react with either antigen; (2) TAXY-6 and TAXY-8 reacted with only XD128; and (3) TAXY-7 reacted with both. In addition, TAXY-1, but not the other MAbs, reacted with a putative tax antigen of an STLV-I-infected cell line, designated RfM26-1. Competitive binding assays showed that TAXY-6 and TAXY-8 did not compete against each other. Sera from HTLV-I-infected humans interfered with the binding of all of these anti-tax1 MAbs. These results indicate that the tax1 antigen and the PX141 express at least 5 distinct epitopes recognized by human and mouse antibodies.

    DOI

  • ISOLATION OF CDNAS FOR DNA-BINDING PROTEINS WHICH SPECIFICALLY BIND TO A TAX-RESPONSIVE ENHANCER ELEMENT IN THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I

    A TSUJIMOTO, H NYUNOYA, T MORITA, T SATO, K SHIMOTOHNO

    JOURNAL OF VIROLOGY   65 ( 3 ) 1420 - 1426  1991年03月

     概要を見る

    One of the gene products of human T-cell leukemia virus type I (HTLV-I), p40tax, activates its own viral transcription in trans through tax-responsive enhancers in viral long terminal repeats. Five species of cDNA clones for proteins that bind to the tax-responsive enhancer element in HTLV-I were isolated from the Jurkat cell library. The beta-galactosidase fusion protein prepared from the lysogen of a clone specifically recognized the cyclic AMP-responsive element in HTLV-I enhancer. The nucleotide sequence of a full-length cDNA clone (TAXREB67) had a coding capacity of 351 amino acids, which contained a basic motif followed by a leucine zipper structure near the carboxy terminus. Its mRNA was detected in human cell lines, including HTLV-I-infected or noninfected hematopoietic cell lines. The mRNA level in Jurkat cells was decreased temporarily by increasing cyclic AMP concentration but increased by increasing Ca2+ concentration. Polyclonal antibodies against the fusion protein specifically recognized a 52-kDa protein in Jurkat cells. Analyses of the function of this protein and its interactions with other cellular factors will be useful to help understand the regulatory mechanism through tax-responsive enhancers in HTLV-I.

  • EXPRESSION OF HTLV-I ENVELOPE PROTEIN FUSED TO HYDROPHOBIC AMINO-TERMINAL PEPTIDE OF BACULOVIRUS POLYHEDRON IN INSECT CELLS AND ITS APPLICATION FOR SEROLOGICAL ASSAYS

    H NYUNOYA, T OGURA, M KIKUCHI, H IWAMOTO, K YAMASHITA, M MAEKAWA, Y TAKEBE, K MIYAMURA, S YAMAZAKI, K SHIMOTOHNO

    AIDS RESEARCH AND HUMAN RETROVIRUSES   6 ( 11 ) 1311 - 1321  1990年11月

  • GENERATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST MULTIPLE EPITOPES ON THE C-TERMINAL HALF OF ENVELOPE GP46 OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I (HTLV-I)

    Y TANAKA, M YASUMOTO, H NYUNOYA, T OGURA, M KIKUCHI, K SHIMOTOHNO, H SHIRAKI, N KURODA, H SHIDA, H TOZAWA

    INTERNATIONAL JOURNAL OF CANCER   46 ( 4 ) 675 - 681  1990年10月

    DOI

  • CHARACTERIZATION OF A PUTATIVE PROMOTER REGION OF THE HUMAN POLY(ADP-RIBOSE) POLYMERASE GENE - STRUCTURAL SIMILARITY TO THAT OF THE DNA POLYMERASE-BETA GENE

    T OGURA, H NYUNOYA, M TAKAHASHIMASUTANI, M MIWA, T SUGIMURA, H ESUMI

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   167 ( 2 ) 701 - 710  1990年03月

    DOI

  • PHOSPHORYLATION OF P40TAX OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I

    H NYUNOYA, K SHIMOTOHNO

    HUMAN RETROVIRUSES /   119   23 - 31  1990年

  • IDENTIFICATION OF A CIS-REGULATORY ELEMENT INVOLVED IN ACCUMULATION OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-II GENOMIC MESSENGER-RNA

    M OHTA, H NYUNOYA, H TANAKA, T OKAMOTO, T AKAGI, K SHIMOTOHNO

    JOURNAL OF VIROLOGY   62 ( 12 ) 4445 - 4451  1988年12月

  • EVIDENCE FOR PHOSPHORYLATION OF TRANS-ACTIVATOR P40X OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 PRODUCED IN INSECT CELLS WITH A BACULOVIRUS EXPRESSION VECTOR

    H NYUNOYA, T AKAGI, T OGURA, S MAEDA, K SHIMOTOHNO

    VIROLOGY   167 ( 2 ) 538 - 544  1988年12月

    DOI

  • NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA FOR HUMAN FIBROBLAST POLY(ADP-RIBOSE) POLYMERASE

    K UCHIDA, T MORITA, T SATO, T OGURA, R YAMASHITA, S NOGUCHI, H SUZUKI, H NYUNOYA, M MIWA, T SUGIMURA

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   148 ( 2 ) 617 - 622  1987年10月

    DOI

  • INVIVO SYNTHESIS OF CARBAMYL-PHOSPHATE FROM NH3 BY THE LARGE SUBUNIT OF ESCHERICHIA-COLI CARBAMYL-PHOSPHATE SYNTHETASE

    SD RUBINO, H NYUNOYA, CJ LUSTY

    JOURNAL OF BIOLOGICAL CHEMISTRY   262 ( 9 ) 4382 - 4386  1987年03月

  • CATALYTIC DOMAINS OF CARBAMYL-PHOSPHATE SYNTHETASE - GLUTAMINE-HYDROLYZING SITE OF ESCHERICHIA-COLI CARBAMYL-PHOSPHATE SYNTHETASE

    SD RUBINO, H NYUNOYA, CJ LUSTY

    JOURNAL OF BIOLOGICAL CHEMISTRY   261 ( 24 ) 1320 - 1327  1986年08月

  • THE GENE CODING FOR CARBAMOYL-PHOSPHATE SYNTHETASE-I WAS FORMED BY FUSION OF AN ANCESTRAL GLUTAMINASE GENE AND A SYNTHETASE GENE

    H NYUNOYA, KE BROGLIE, CJ LUSTY

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   82 ( 8 ) 2244 - 2246  1985年

    DOI

  • CHARACTERIZATION AND DERIVATION OF THE GENE CODING FOR MITOCHONDRIAL CARBAMYL-PHOSPHATE SYNTHETASE-I OF RAT

    H NYUNOYA, KE BROGLIE, EE WIDGREN, CJ LUSTY

    JOURNAL OF BIOLOGICAL CHEMISTRY   260 ( 16 ) 9346 - 9356  1985年

  • SEQUENCE OF THE SMALL SUBUNIT OF YEAST CARBAMYL-PHOSPHATE SYNTHETASE AND IDENTIFICATION OF ITS CATALYTIC DOMAIN

    H NYUNOYA, CJ LUSTY

    JOURNAL OF BIOLOGICAL CHEMISTRY   259 ( 15 ) 9790 - 9798  1984年

  • EFFECTS OF SUGARS AND POLYOLS ON BASIDIOCARP FORMATION IN A PHOSPHOGLUCOSE ISOMERASE MUTANT OF COPRINUS-CINEREUS

    H NYUNOYA, T TAKEMARU, T ISHIKAWA

    CANADIAN JOURNAL OF MICROBIOLOGY   30 ( 1 ) 45 - 51  1984年

  • DNA-SEQUENCE OF THE CARA GENE - TANDEM PROMOTERS, RESPECTIVELY CONTROLLED BY ARGININE AND THE PYRIMIDINES, REGULATE THE SYNTHESIS OF CARBAMOYLPHOSPHATE SYNTHETASE IN ESCHERICHIA-COLI-K-12

    J PIETTE, H NYUNOYA, CJ LUSTY, R CUNIN, G WEYENS, M CRABEEL, D CHARLIER, N GLANSDORFF, A PIERARD

    ARCHIVES INTERNATIONALES DE PHYSIOLOGIE DE BIOCHIMIE ET DE BIOPHYSIQUE   91 ( 4 ) B113 - B114  1983年

  • YEAST CARBAMYL-PHOSPHATE SYNTHETASE - STRUCTURE OF THE YEAST GENE AND HOMOLOGY TO ESCHERICHIA-COLI CARBAMYL-PHOSPHATE SYNTHETASE

    CJ LUSTY, EE WIDGREN, KE BROGLIE, H NYUNOYA

    JOURNAL OF BIOLOGICAL CHEMISTRY   258 ( 23 ) 4466 - 4477  1983年

  • DNA-SEQUENCE OF THE CARB GENE OF ESCHERICHIA-COLI CODING FOR THE LARGE SUBUNIT OF CARBAMYL-PHOSPHATE SYNTHETASE

    H NYUNOYA, CJ LUSTY

    FEDERATION PROCEEDINGS   42 ( 7 ) 2116 - 2116  1983年

  • EFFECTS OF 2-DEOXY-D-GLUCOSE AND QUINIDINE ON THE FRUITING BODY FORMATION IN COPRINUS-MACRORHIZUS

    UNO, I, H NYUNOYA, T ISHIKAWA

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   27 ( 3 ) 219 - 228  1981年

    DOI

  • RELATIONSHIP BETWEEN FRUITING BODY FORMATION AND CARBOHYDRATE-METABOLISM IN A MUTANT (PGI) OF COPRINUS-MACRORHIZUS

    H NYUNOYA, T TAKEMARU, T ISHIKAWA

    JAPANESE JOURNAL OF GENETICS   55 ( 6 ) 481 - 481  1980年

  • EFFECT OF 2-DEOXY-D-GLUCOSE ON THE INDUCTION OF TYROSINASE IN COPRINUS-MACRORHIZUS

    H NYUNOYA, T ISHIKAWA

    JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY   26 ( 3 ) 229 - 238  1980年

    DOI

  • RELATIONSHIP BETWEEN DEFICIENCY OF PHOSPHOGLUCOSE ISOMERASE IN COPRINUS-MACRORHIZUS AND FRUITING BODY FORMATION

    H NYUNOYA, T ISHIKAWA

    JOURNAL OF BACTERIOLOGY   144 ( 1 ) 432 - 434  1980年

  • Control of unusual hyphal morphology in a mutant of Coprinus macrorhizus

    H. Nyunoya, T. Ishikawa

    Jap. J. Genet. Vol. 54, p. 11-22    1979年

    DOI

▼全件表示

書籍等出版物

  • バイオロジカルクリーンルームの設計・維持管理と作業員教育(遺伝子研究施設における清浄度基準とその関連法規対応)

    丹生谷 博( 担当: 共著)

    技術情報協会  2018年02月

  • ゲノム情報解析 ー 次世代シーケンサーの最新の方法と応用

    石井 一夫, 富田 因則, 丹生谷 博, 大藤 道衛( 担当: 共訳)

    エヌ・ティー・エス  2016年03月

  • 遺伝子治療・診断の最先端技術と新しい医薬品・診断薬の開発(植物への遺伝子導入時における適切なプロトコール,トラブル回避策)

    丹生谷( 担当範囲: 植物への遺伝子導入時における適切なプロトコール,トラブル回避策)

    技術情報協会  2014年05月

  • 新バイオの扉ー未来を拓く生物工学の世界ー

    丹生谷博( 担当: 共著,  担当範囲: 遺伝子組換え作物)

    裳華房  2013年

  • RADIOISOTOPES(月刊誌)「植物ウイルスにコードされる蛋白質と相互作用する植物因子」

    日本アイソトープ協会  2010年

  • 食品の安全性(「課題に挑む」シリーズ[29] -技術士のソリューション-)

    日刊工業新聞  2010年

  • バイオテクニシャン(季刊誌)「理科教員のための遺伝子組換え実験教育研修会誌上再現」

    NPO法人 日本バイオ技術教育学会  2009年 ISBN: 9784902600353

  • フードリサーチ(月刊誌)「遺伝子組換え食品の開発と安全性評価」

    (株)食品研究社  2009年

  • 技術士(月刊誌)「遺伝子組換え食品の安全性評価と品質表示基準」

    (社)日本技術士会  2009年

  • バイオ実験 誰もがつまずく失敗&ナットク解決法「R I 実験の心構え」及び「遺伝子組換え実験を行う心構え」

    羊土社  2008年 ISBN: 9784758107273

  • 廣川タンパク質化学第4巻 酵素4 . 5イソメラーゼ・リガーゼ「カルバモイルリン酸シンテターゼ(アンモニア依存型)」及び「カルバ モイルリン酸シンテターゼ(グルタミン依存型)」

    廣川書店  2003年

  • バイオの扉「ウイルスと人間のバイオ戦略」

    ( 担当: 共著)

    裳華房  2000年 ISBN: 4785358327

  • バイオサイエンスとインダストリー(隔月刊誌)「Thiobacillus thioparus が生産する新規なチオシアネート加水分解酵素」

    片山葉子, 丹生谷 博( 担当: 共著)

    バイオインダストリー協会  1999年

  • 分子細胞生物学辞典

    東京化学同人  1997年 ISBN: 9784807906871

  • 組織培養「バキュロウイルスベクター」

    丹生谷 博, 前田 進( 担当: 共著)

    日本組織培養学会  1988年

  • 蛋白質・核酸・酵素(月刊誌)「カルバミルリン酸合成酵素の遺伝子構造と分子進化」

    共立出版  1986年

▼全件表示

Misc

  • ウイルス防御応答における抵抗性遺伝子Nのイントロンの役割

    佐々木信光, 多久和夫, 丹生谷博

    植物感染生理談話会論文集   ( 53 ) 27 - 36  2018年08月  [査読有り]  [招待有り]

  • ゲノムリテラシー教育教材の開発と一般市民向けワークショップパッケージ「私たちのDNA」のデザインと実践

    大藤 道衛, 丹生谷 博, 佐々 義子

    遺伝 : 生物の科学   71 ( 3 ) 269 - 278  2017年05月

    CiNii

  • 教育目的遺伝子組換え実験教育研修会の10年間の活動

    小野道之, 大藤道衛, 齋藤淳一, 中島春紫, 佐藤由紀夫, 笹川由紀, 正木春彦, 丹生谷博, 鎌田博

    日本植物学会大会研究発表記録   75th   244  2011年09月

    J-GLOBAL

  • タバコモザイクウイルス(TMV)抵抗性遺伝子産物NおよびN類似タンパク質の機能ドメインの解析

    GAO J. S., 鐘ケ江弘美, 滝沢香, 小長谷賢一, 岡野陽介, 林尚美, 佐々木信光, 松下保彦, 丹生谷博

    日本植物病理学会報   73 ( 3 )  2007年

    J-GLOBAL

  • チオシアネート加水分解酵素の組換え体発現―活性化タンパク質P15Kの機能

    尾高雅文, 片岡慎吾, 荒川孝俊, 堀祥太, 片山葉子, 松下保彦, 中山洋, 丹生谷博, 堂前直, 前田瑞夫, 養王田正文

    酵素工学研究会講演会講演要旨集   56th   48  2006年

    J-GLOBAL

  • システイン酸化体を配位子とする新規金属酵素の反応機構解析

    尾高雅文, 中山洋, 橋本花那子, 片山葉子, 河野能顕, 養王田正文, 丹生谷博, 長棟輝行, 遠藤勲

    生化学   73 ( 8 ) 633  2001年08月

    J-GLOBAL

  • 植物RNAウイルスの移行蛋白質と相互作用する宿主因子のcDNAクローニング

    松下保彦, 丹生谷 博

    植物感染生理談話会論文集 第36号, p.132-139 (ISSN 1345-8086) 日本植物病理学会    2000年

  • エンドウにおけるSCRホモログの単離とその発現解析

    佐々 奈緒美, 松下 保彦, 丹生谷 博, 村山 肇子, 山田 晃弘, 中村 輝子

    宇宙生物科学 = Biological sciences in space   13 ( 3 ) 284 - 285  1999年09月

    CiNii

  • ISOLATION AND CHARACTERIZATION OF cDNAs ENCODING PROTEINS THAT INTERACT WITH THE MOVEMENT PROTEIN OF TOMATO MOSAIC VIRUS

    MATSUSHITA Yasuhiko, DEGUCHI Masakazu, YOUDA Masaru, WATANABE Yuichiro, NISHIGUCHI Masamichi, NYUNOYA Hiroshi

    Plant and cell physiology   40   s110 - s110  1999年03月

    CiNii

  • IDENTIFICATION OF A PROTEIN KINASE THAT PHOSPHORYLATES A RECOMBINANT MOVEMENT PROTEIN OF TOMATO MOSAIC VIRUS

    MATSUSHITA Yasuhiko, HANAZAWA Kohtarou, YOSHIOKA Kuniaki, KAWAKAMI Shigeki, WATANABE Yuichiro, NISHIGUCHI Masamichi, NYUNOYA Hiroshi

    Plant and cell physiology   39   S145 - S145  1998年05月

    CiNii

▼全件表示

受賞

  • 平成28年度論文賞

    2016年03月   日本植物病理学会  

    受賞者: 佐々木信光, 宍倉竜樹, 丹生谷 博

  • 会長表彰

    2013年06月   日本技術士会  

    受賞者: 丹生谷 博

  • 特別賞

    2011年09月   日本植物学会  

    受賞者: 鎌田博, 丹生谷博, その他を含むグループ

共同研究・競争的資金等の研究課題

  • 植物ウイルスの感染と植物の応答

    研究期間:

    1995年
    -
    2004年
     

  • Plant virus and plant response to the infection

    研究期間:

    1995年
    -
    2004年
     

  • 核タンパク質のcDNAクローニング

  • 微生物の有用遺伝子クローニング

  • 植物遺伝子の機能解析

  • 高等植物の分子生物学

  • cDNA cloning of nuclear proteins

  • Gene cloning of microbial proteins

  • Functional analysis for plant genes

  • Molecular Biology of Higher Plant

▼全件表示

講演・口頭発表等

  • CRISPR/Cas9 ゲノム編集によるBBF遺伝子ノックアウトタバコの作製

    鈴木大河, 鈴木新, 竹田篤史, 丹生谷博, 松下保彦, 佐々木信光

    令和2年度日本植物病理学会関東部会  

    発表年月: 2020年09月

    開催年月:
    2020年09月
     
     
  • 転写物の蓄積とウイルス感染抑制における抵抗性遺伝子Nのイントロン1と2の重要性

    佐々木信光, 池田千尋, 松下保彦, 丹生谷 博

    令和2年度日本植物病理学会大会  

    発表年月: 2020年03月

    開催年月:
    2020年03月
     
     
  • 病原性青枯病菌への耐病性におけるタバコDOF型転写因子BBF3の役割

    鈴木大河, 鈴木美緒, 曳地康史, 丹生谷博, 松下保彦, 佐々木信光

    令和2年度日本植物病理学会大会  

    発表年月: 2020年03月

    開催年月:
    2020年03月
     
     
  • 過剰発現タバコを用いたDof 型転写 因子BBF2 の病害抵抗性における機能の解析

    鈴木 新, 仁藤史乃, 曵地康史, 松浦恭和, 池田陽子, 丹生谷博, 松下保彦, 佐々木信光

    平成31年度日本植物病理学会大会  

    発表年月: 2019年03月

  • ウイルス抵抗性遺伝子N のイントロ ン4 に由来するsmall RNA の推定標的遺伝子の解析

    白井梨花子, 丹生谷博, 松下保 彦, 佐々木信光

    平成31年度日本植物病理学会大会  

    発表年月: 2019年03月

  • イントロンを介した抵抗性遺伝子Nの転写物量調節機構の解析

    宮崎 紡希, 池田 千紘, 多久 和夫, 山家 美歩, 丹生谷 博, 松下 保彦, 佐々木 信光

    第41回日本分子生物学会年会  

    発表年月: 2018年11月

  • 抵抗性遺伝子Nのイントロンによる転写量調節機構の解析

    宮崎紡希, 池田千紘, 多久和夫, 山家美歩, 丹生谷博, 松下保彦, 佐々木信光

    第53回植物感染生理談話会  

    発表年月: 2018年08月

  • ゲノム編集によるBBF転写因子遺伝子ノックアウトタバコの作出

    鈴木新, 井手口真也, 竹田篤史, 丹生谷博, 松下保彦, 佐々木信光

    第53回植物感染生理談話会  

    発表年月: 2018年08月

  • トマトモザイクウイルス感染におけるタバコの膜タンパク質NtREMの役割

    佐々木信光, 高島永太, 丹生谷博

    平成30年度 日本植物病理学会大会  

    発表年月: 2018年03月

  • タバコのウイルス抵抗性遺伝子Nのイントロンは転写物量の増大に関与する

    多久 和夫, 松沢 健太, 向井(岡村) 篤, 佐々木 信光, 丹生谷 博

    平成30年度 日本植物病理学会大会  

    発表年月: 2018年03月

  • ゲノム編集技術によるBBF2およびBBF3遺伝子ノックアウトタバコの作出

    鈴木 新, 井手口 真也, 竹田 篤史, 丹生谷 博, 松下 保彦, 佐々木 信光

    第41回日本分子生物学会年会  

    発表年月: 2018年

  • ウイルス抵抗性遺伝子Nの選択的ポリアデニル化シグナルの機能解析

    吉本 菜苗, 丹生谷 博, 松下 保彦, 佐々木 信光

    第41回日本分子生物学会年会  

    発表年月: 2018年

  • ウイルス抵抗性遺伝子N のイントロンに由来するsmall RNAの標的候補遺伝子の解析

    白井 梨花子, 丹生谷 博, 松下 保彦, 佐々木 信光

    第41回日本分子生物学会年会  

    発表年月: 2018年

  • トマトモザイクウイルスの増殖過程におけるタバコの膜タンパク質レモリンの関与

    高島 永太, 丹生谷 博, 松下 保彦, 佐々木 信光

    2017年度生命科学系学会合同年次大会(ConBio2017)  

    発表年月: 2017年11月

  • タバコモザイクウイルスエリシターに対する応答における抵抗性遺伝子Nのタンパク質とイントロンの役割

    多久 和夫, 岡村 篤, 佐々木 信光, 丹生谷 博

    平成29年度 日本植物病理学会大会  

    発表年月: 2017年

  • トマトモザイクウイルスの複製タンパク質と移行タンパク質はタバコの膜タンパク質レモリンの凝集体形成を引き起こす

    髙島 永太, 佐々木 信光, 丹生谷 博

    平成29年度 日本植物病理学会大会  

    発表年月: 2017年

  • 一過的遺伝子発現系を利用したウイルス抵抗性遺伝子Nの転写制御機構の解析

    多久和夫, 岡村篤, 佐々木信光, 丹生谷博

    平成28年度日本植物病理学会大会  

    発表年月: 2016年

  • 抵抗性因子Nとタバコモザイクウイルスエリシターの共発現がトマトモザイクウイルス感染を抑制するメカニズムの解析

    村上智哉, 高岡万純, 佐々木信光, 丹生谷博

    平成28年度日本植物病理学会大会  

    発表年月: 2016年

  • ウイルス抵抗性遺伝子N のエリシター応答性転写制御におけるNタンパク質とイントロンの重要性

    多久和夫, 岡村篤, 佐々木信光, 丹生谷博

    第39回日本分子生物学会年会  

    発表年月: 2016年

  • ウイルス抵抗性遺伝子Nのイントロン配列の遺伝子発現およびエリシターによる細胞死誘導における役割

    山家美歩, 松沢健太, 佐々木信光, 丹生谷博

    平成27年度 日本植物病理学会大会  

    発表年月: 2015年

  • 形質転換タバコを用いたタバコDof転写因子BBF3の病害抵抗性における機能解析

    鈴木美緒, 森友花, 佐々木信光, 曳地康史, 丹生谷博

    第38回日本分子生物学会年会  

    発表年月: 2015年

  • タバコモザイクウイルスのヘリカーゼ領域の一過的発現による近縁トバモウイルスに対する感染抑制効果の解析

    村上智哉, 高岡万純, 佐々木信光, 丹生谷博

    第38回日本分子生物学会年会  

    発表年月: 2015年

  • ウイルス抵抗性遺伝子及び防御応答関連遺伝子の転写誘導に関与するタバコの3種のBBF/Dofタンパク質

    佐々木信光, 松丸昌道, 小平将太, 仲田啓子, 中山逸平, 山口昂哉, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2014年11月

  • Induction of virus resistance by the transient expression of the tobacco resistance factor N and a tobamovirus eilicitor in Nicotiana benthamiana

    T. Murakami, N. Sasaki, H. Nyunoya

    3rd Korea-Japan Joint Symposium on Plant Pathology  

    発表年月: 2014年10月

  • Functional analysis of tobacco transcription factor BBF1 by using transgenic tobacco plants

    M. Suzuki, K. Shigeta, N. Sasaki, H. Nyunoya

    3rd Korea-Japan Joint Symposium on Plant Pathology  

    発表年月: 2014年10月

  • トマトモザイクウイルス移行タンパク質との一過的共発現下における126-kDa 複製タンパク質の細胞内局在および動態の解析

    佐々木信光, 宍倉竜樹, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2014年06月

  • タバコ抵抗性因子Nのスプライシングバリアントはウイルスエリシター誘導性の防御応答を負に制御する

    佐々木信光, 高岡万純, 丹生谷 博

    日本植物生理学会年会  

    発表年月: 2014年03月

  • CYTOPLASMIC BODIES OF THE TOMATO MOSAIC VIRUS 126-KDA REPLICATION PROTEIN MOVE INTRACELLULARLY TOGETHER WITH THE MOVEMENT PROTEIN

    Nobumitsu Sasaki, Ryuki Shishikura, Hiroshi Nyunoya

    IS-MPMI2014 XVI International Congress  

    発表年月: 2014年

  • THE TRUNCATED PROTEIN NTR ENCODED BY THE TOBACCO RESISTANCE GENE N ACTS AS A NEGATIVE REGULATOR AGAINST THE NORMAL PROTEIN N IN THE DEFENSE RESPONSE

    Hiroshi Nyunoya, Masumi Takaoka, Nobumitsu Sasaki

    IS-MPMI2014 XVI International Congress  

    発表年月: 2014年

  • ウイルス抵抗性遺伝子Nの転写制御因子候補BTF3のクローニングと機能解析

    宮本寛夫, 小平将太, 石川英明, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2013年12月

  • たばこDof転写因子BBF1, BBF2およびBBF3のウイルスエリシター応答性過敏感細胞死の誘導および防御関連遺伝子発現における機能の比較

    中山逸平, 仲田啓子, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2013年12月

  • タバコDof転写因子BBFタンパク質間相互作用のin vitro解析

    山口昂哉, Haque MD, 仲田積美, 仲田啓子, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2013年12月

  • ヒトゲノムリテラシー教材の開発と市民向けワークショップ「私たちのDNA」のパッケージ化

    大藤道衛, 丹生谷 博, 佐々義子

    日本分子生物学会年会  

    発表年月: 2013年12月

  • Overexpression of the Dof protein in tobacco leads to transcriptional activation of the resistance gene N, suggesting potential roles in the regulation of hypersensitive response to TMV

    M. Takano, S, Odaira, M. A. Haque, N. Sasaki, H. Nyunoya

    24th International Conference on Arabidopsis Research  

    発表年月: 2013年06月

  • タバコDofタンパク質BBF1の一過的過剰発現における防御関連遺伝子の発現解析

    高野真由美, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2013年03月

  • 抵抗性因子Nのスプライシングバリアントの一過的過剰発現はエリシター誘導性の過敏感細胞死およびウイルス抵抗性を抑制する

    高岡万純, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2013年03月

  • KELPホモログの一過的過剰発現によるイネウイルスの細胞間移行タンパク質の輸送機能に対する抑制効果

    平栗章弘, 根津修, 清水巧, 一木珠樹, 木村敏博, 佐々木信光, 丹生谷 博, 笹谷孝英

    日本植物病理学会大会  

    発表年月: 2012年03月

  • 酵母細胞を利用したシロイヌナズナのMCA1とMCA2の低浸透圧応答性の研究

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本植物生理学会年会  

    発表年月: 2012年03月

  • N遺伝子による防御応答におけるタバコDofタンパク質BBF2の機能解析

    小平将太, 松丸昌道, 仲田積美, 佐々木信光, 丹生谷 博

    日本植物生理学会年会  

    発表年月: 2012年03月

  • Transcriptional activation of the N gene and increase of ROS generation by transient overexpression of tobacco Dof protein BBF1

    Mayumi Takano, Md. Ashraful Haque, Nobumitsu Sasaki, Hiroshi Nyunoya

    The 2nd Korea-Japan Joint Symposium  

    発表年月: 2012年

  • Overexpression of tobacco Dof transcription factor enhances transcriptional activation of the virus resistance gene N and ROS generation

    Mayumi Takano, Md. Ashraful Haque, Nobumitsu Sasaki, Hiroshi Nyunoya

    S-MPMI 2012 XV International Congress  

    発表年月: 2012年

  • The role of a splice variant product of the virus resistance gene N in the induction of hypersensitive response

    Masumi Takaoka, Mayumi Takano, Md. Ashraful Haque, Nobumitsu Sasaki, Hiroshi Nyunoya

    IS-MPMI 2012 XV International Congress  

    発表年月: 2012年

  • Involvement of Dof proteins in the transcriptional activation of the tobacco resistance gene N by the tobacco mosaic virus elicitor and its implication for the induction of hypersensitive response

    Hiroshi Nyunoya, Mayumi Takano, Shota Odaira, Md. Ashraful Haque, Nobumitsu Sasaki

    BIT's 2nd Annual World Congress of Microbe-2012  

    発表年月: 2012年

  • シロイヌナズナのCa2+透過性機械受容チャネル候補MCA1とMCA2のCa2+取り込み活性に重要な領域の特定

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本分子生物学会年会  

    発表年月: 2011年12月

  • TMV抵抗性遺伝子Nのスプライシングバリアント産物のHR誘導における役割

    高岡万純, 高野真由美, ハーク アシュラフル, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2011年12月

  • ウイルス抵抗性遺伝子Nを介した過敏感応答の誘導におけるタバコDofタンパク質BBF1の機能解析

    高野真由美, ハーク アシュラフル, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2011年12月

  • タバコ転写因子BBF1の一過的発現はウイルス抵抗性遺伝子Nの転写とTMVエリシターによる過敏感細胞死を促進させる

    高野真由美, Md. Ashraful Haque, 佐々木信光, 丹生谷 博

    日本植物病理学会関東部会  

    発表年月: 2011年09月

  • 転写共役因子BcKELPを過剰発現する形質転換タバコはトマトモザイクウイルスに対して抵抗性を示す

    小田原達郎, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2011年03月

  • ウイルス抵抗性遺伝子Nのエリシター応答配列におけるタバコDofタンパク質BBF1の認識配列の解析

    小平将太, アシュラフルハーク, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2011年03月

  • タバコのDofタンパク質BBF1の一過的過剰発現はタバコモザイクウイルス抵抗性遺伝子Nのプロモーターの活性化をもたらす

    高野真由美, アシュラフルハーク, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2011年03月

  • イネグラッシースタントウイルスのpC6とイネ縞葉枯ウイルスのpC4は細胞間移行能欠損トマトモザイクウイルスの細胞間移行を相補する

    平栗章弘, 根津修, 清水巧, 一木珠樹, 木村敏博, 佐々木信光, 丹生谷 博, 笹谷孝英

    日本植物病理学会大会  

    発表年月: 2011年03月

  • 酵母細胞を利用したシロイヌナズナのMCA1とMCA2のCa2+取り込み活性に重要な領域の特定

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本植物生理学会年会  

    発表年月: 2011年03月

  • Interfered cell-to-cell movement of Tomato mosaic virus in transgenic tobacco plants over-expressing BcKELP, a binding factor for viral movement proteins

    Nobumitsu Sasaki, Tatsuro Odawara, Hiroshi Nyunoya

    XV International Congress of Virology  

    発表年月: 2011年

  • The gene 3-encoded cell-to-cell movement protein is a virus structural protein of rice transitory yellowing virus

    Akihiro Hiraguri, Osamu Netsu, Takumi Shimizu, Tamaki Uehara-Ichiki, Toshihiro Omura, Nobumitsu Sasaki, Hiroshi Nyunoya, Takahide Sasaya

    XV International Congress of Virology  

    発表年月: 2011年

  • Virus resistance of transgenic tobacco plants expressing a movment protein-binding protein.

    Nobumitsu Sasaki, Tatsuro Odawara, Hiroshi Nyunoya

    8th Solanaceae and 2nd Cucurbitaceae Joint Conference  

    発表年月: 2011年

  • Overexpression of a Dof transcription factor accelerates hypersensitive response to the elicitor of Tobacco mosaic virus through the upregulation of the tobacco resistance gene N

    Mayumi Takano, Shota Odaira, Md.A.Haque, Nobumitsu Sasaki, Hiroshi Nyunoya

    8th Solanaceae and 2nd Cucurbitaceae Joint Conference  

    発表年月: 2011年

  • Transcriptional transactivation of tobacco resistance gene N for Tobacco mosaic virus through its own product N protein

    Hiroshi Nyunoya, Nobumitsu Sasaki, Md. Ashuraful Haque, Mayumi Takano, Shota Odaira  [招待有り]

    BIT's 1st Annual World Congress of Microbes-2011  

    発表年月: 2011年

  • Brassica campestris由来の転写共役因子KELPを過剰発現する形質転換タバコにおける植物細胞間移行の遅延

    小田原達郎, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2010年12月

  • タバコDofタンパク質BBF1と抵抗性因子Nのウイルスエリシター応答配列との結合解析

    小平将太, アシュラフルハーク, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2010年12月

  • タバコのDofタンパク質BBF1の一過的過剰発現はウイルス抵抗性N遺伝子のプロモーター活性を増強する

    高野真由美, アシュラフルハーク, 佐々木信光, 丹生谷 博

    日本分子生物学会年会  

    発表年月: 2010年12月

  • しろイヌナズナのCa2+透過性機械受容チャネル候補の酵母発現系を用いた構造と機能の解析

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本分子生物学会年会  

    発表年月: 2010年11月

  • ヒメツリガネゴケにおけるブラシノステロイドの働き

    大橋裕樹, 津森康一郎, 丹生谷 博, 笠原賢洋

    植物化学調節学会  

    発表年月: 2010年10月

  • 環境ストレス応答に関与するCa(2+)透過性機械受容チャネル候補MCA1およびMCA2

    中野正貴, 飯田和子, 原茂恵美子, 今村朋美, 森研堂, 丹生谷博, 飯田秀利

    日本植物学会第74回大会  

    発表年月: 2010年09月

  • Brassica campestris由来のKELPを過剰発現する形質転換タバコにおけるTomato mosaic virusの細胞間移行の遅延

    小田原達郎, 佐々木信光, 丹生谷 博

    日本植物病理学会大会  

    発表年月: 2010年04月

  • タバコのDofタンパク質BBF1は抵抗性遺伝子Nのウイルスエリシター応答配列と結合する

    小平将太, Ashraful, H, 佐々木信光, 丹生谷博

    日本植物病理学会大会  

    発表年月: 2010年04月

  • タバコモザイクウイルス抵抗性遺伝子Nの転写増強に関与するエリシター応答配列の同定

    佐々木信光, Haque, Md. A, 松丸昌道, 小平将太, 丹生谷博

    日本植物病理学会大会  

    発表年月: 2010年04月

  • 出芽酵母を用いたシロイヌナズナのCa2+透過性機械受容チャネル候補の構造と機能の解析

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本植物生理学会年会(2010)  

    発表年月: 2010年03月

  • Tobacco transcription factor Dof proteins mediate the promoter activation of the resistance gene N against Tobacco mosaic virus [

    Nobumitsu Sasaki, Md. A Haque, Mayumi Takano, Shota Odaira, Hiroshi Nyunoya

    Cold Spring Harbor Asia Conferences  

    発表年月: 2010年

  • Molecular Study on Arabidopsis Ca2+-permeable mechanosensitive channel candidates using yeast

    Nakano, M, Iida, K, Nyunoya, H, Iida, H

    International Congress of Arabidopsis Research 2010  

    発表年月: 2010年

  • Involvement of tobacco transcription factor Dof proteins in the promoter activation of the resistance gene N against Tobacco mosaic virus

    International Congress of Arabidopsis Research 2010  

    発表年月: 2010年

  • Structure-function analysis of Arabidopsis Ca2+-permeable mechanosensitive channel candidates using a yeast Ca2+ channel mutant

    M. Nakano, K. Iida, H. Nyunoya, H. Iida

    第32回日本分子生物学会  

    発表年月: 2009年12月

  • Arabidopsis thaliana KELP ホモログの一過的過剰発現による Tomato mosaic virus の細胞間移行に対する抑制効果

    小田原達郎, 佐々木信光, 丹生谷 博

    第32回日本分子生物学会  

    発表年月: 2009年12月

  • Involvement of tobacco Dof proteins in the transcriptional activation of the resistance gene for Tobacco mosaic virus.

    Haque, M. A, N. Sasaki, M. Matsumaru, H. Nyunoya

    第32回日本分子生物学会  

    発表年月: 2009年12月

  • Interference with Tomato mosaic virus cell-to-cell movement by transient overexpression of Arabidopsis thaliana KELP homologs from different plant species

    T. Odawara, H. Nyunoya, S. Sasaki

    The 1st Japan-Korea Joint Symposium (2009)  

    発表年月: 2009年10月

  • Elicitor-responsive 20-bp element of the Tobacco mosaic virus resistance gene N

    M. A. Haque, N. Sasaki, H. Kanegae, J. Gao, H. Nyunoya

    XIV International Congress on Molecular Plant-Microbe Interactions  

    発表年月: 2009年07月

  • KELPホモログの一過的過剰発現によるトマトモザイクウイルスの細胞間移行に対する抑制効果

    小田原達郎, 佐々木信光, 丹生谷博

    日本植物病理学会大会  

    発表年月: 2009年03月

  • シロイヌナズナのCa2+透過性機械受容チャネル候補の出芽酵母を利用した構造機能解析

    中野正貴, 飯田和子, 丹生谷 博, 飯田秀利

    日本植物生理学会年会  

    発表年月: 2009年03月

  • ヒメツリガネゴケにおけるブラシノステロイドの働き

    津森康一郎, 丹生谷博, 笠原賢洋

    日本植物生理学会年会  

    発表年月: 2009年03月

  • Study on the regulation of the elicitor-responsive expression of Tobacco mosaic virus resistance

    発表年月: 2009年

  • タバコモザイクウイルス抵抗性遺伝子Nのシス調節エレメントの同定

    日本分子生物学会・日本生化学会合同大会  

    発表年月: 2008年

  • Two Novel N-like Genes Encoding Functionally Competent Domains to Induce Hypersensitive Response.

    9th International Congress of Plant Pathology  

    発表年月: 2008年

  • Overexpression of putative transcriptional coactivator KELP interferes with virus movement.

    9th International Congress of Plant Pathology  

    発表年月: 2008年

  • 微生物群集解析による嫌気性ミクロフローラへの微生物ストレス処理の評価

    農業施設学会年次大会  

    発表年月: 2008年

  • Effect of bacterial stress on hydrogen fermentation microflora

    Hiroyuki Kashima, Hiroshi Nyunoya, Michiei Oto, Seishu Tojo

    ASABE 2008 Annual International Meeting  

    発表年月: 2008年01月

  • Plant Resistance Genes for Pathogens - N gene for TMV -

    (招待講演)安徽省農業科学院蚕桑研究所  

    発表年月: 2008年

  • マメザクラ極小盆栽を用いた宇宙実験による樹木の機能解析

    第24回宇宙利用シンポジウム  

    発表年月: 2008年

  • Overexpression of the tobacco NTERF3 gene induces hypersensitive response-like cell death in plants.

    14th International Congress of Virology  

    発表年月: 2008年

  • KELPの一過的過剰発現によるトマトモザイクウイルス移行タンパク質の細胞内局在の変化

    日本植物病理学会大会  

    発表年月: 2008年

  • タバコモザイクウイルス感染時のタバコNtERF3遺伝子の発現解析と一過的過剰発現による過敏感細胞死の誘導

    日本植物病理学会大会  

    発表年月: 2008年

  • シロイヌナズナのCa2+透過性機械受容チャネル候補の出芽酵母を利用した構造機能解析

    日本植物生理学会年会  

    発表年月: 2008年

  • Overexpression of the tobacco NTERF3 gene induces hypersensitive response-like cell death in plants.

    14th International Congress of Virology  

    発表年月: 2008年

  • Identification of a cis regulatory element of the Tobacco mosaic virus resistance gene N

    BMB2008  

    発表年月: 2008年

  • Two Novel N-like Genes Encoding Functionally Competent Domains to Induce Hypersensitive Response.

    9th International Congress of Plant Pathology  

    発表年月: 2008年

  • Overexpression of putative transcriptional coactivator KELP interferes with virus movement.

    9th International Congress of Plant Pathology  

    発表年月: 2008年

  • Plant Resistance Genes for Pathogens - N gene for TMV -

    発表年月: 2008年

  • タバコNtERF3遺伝子の一過的過剰発現による植物過敏感細胞死の誘導

    第30回日本分子生物学会年会  

    発表年月: 2007年

  • タバコモザイクウイルス抵抗性遺伝子(N)とN類似遺伝子のキメラcDNA作製による過敏感応答誘導能の解析

    第30回日本分子生物学会年会  

    発表年月: 2007年

  • トマトモザイクウイルス移行タンパク質と相互作用するAtKELPの過剰発現によるウイルス移行の抑制

    日本植物病理学会大会  

    発表年月: 2007年

  • タバコモザイクウイルス(TMV)抵抗性遺伝子NおよびN類似タンパク質の機能ドメインの解析

    日本植物病理学会大会  

    発表年月: 2007年

  • 宇宙環境における樹木の形態形成と機能分子および樹木の応用利用

    第23回 宇宙利用シンポジウム  

    発表年月: 2007年

  • ウイルス抵抗性遺伝子N類似遺伝子の過敏感応答誘導性に関する解析

    平成19年度植物感染生理談話会(ポスター)  

    発表年月: 2007年

  • Isolation of cDNA clones similar to the virus resistance gene N from Nicotiana tabacum.

    Meiji University International Symposium "Plant Immunity: From MAMPs/PAMPS Recognition to Gene-for-Gene Resistance"  

    発表年月: 2007年

  • Functional compounds and morphogenesis of tree, and its applied utilization

    Space Utilization Research  

    発表年月: 2007年

  • Tomato mosaic virus movement protein interacts with tobacco RIO kinase that is phosphorylated by protein kinase CK2

    8th International Congress of Plant Molecular Biology (POS-THU-394)  

    発表年月: 2006年

  • 遺伝子リテラシー教育

    第24回日本植物細胞分子生物学会(つくば)大会・シンポジウム  

    発表年月: 2006年

  • Japanese flowering cherry tree as a woody plant candidate grown in space

    Committee on Space Research 36th COSPAR Scientific Assembly  

    発表年月: 2006年

  • 宇宙における樹木ー宇宙における樹木形態形成に関与する環境機能分子および樹木の応用利用

    第22回 宇宙利用シンポジウム  

    発表年月: 2006年

  • Tobacco RIO kinase (NtRIO) interacts with Tomato mosaic virus movement protein depending on the phosphorylation status of NtRIO

    Protein Phosphorylation in Plant Signaling  

    発表年月: 2005年

  • Expression of gibberellin biosynthetic gene in the weeping type of Japanese cherry

    第46回日本植物生理学会大会  

    発表年月: 2005年

  • Expression of gibberellin biosynthetic gene in the weeping type of Japanese cherry

    発表年月: 2005年

  • Tobacco mosaic virus抵抗性遺伝子産物と14-3-3タンパク質の相互作用

    日本植物病理学会大会  

    発表年月: 2004年

  • Members of 14-3-3 protein isoforms interact with Tobacco mosaic virus resistance gene product N

    "Plant Immunity" Signaling to acquired resistance  

    発表年月: 2004年

  • CBP2ホモログ(AtOLP)のシロイヌナズナ形質転換体の根におけるサイトカイニン応答

    第45回日本植物生理学会大会  

    発表年月: 2004年

  • ブラシノステロイド受容体キナーゼによりリン酸化を受けるcDNA産物の機能解析

    第22回日本植物細胞分子生物学会大会  

    発表年月: 2004年

  • Members of 14-3-3 protein isoforms interact with Tobacco mosaic virus resistance gene product N

    "Plant Immunity" Signaling to acquired resistance  

    発表年月: 2004年

  • Cytokinin response in root of Arabidopsis thaliana over-expressed CBP2 homolog (AtCBP)

    発表年月: 2004年

  • Expression and characterization of the recombinant thiocyanate hydrolase

    第 76 回日本生化学会大会  

    発表年月: 2003年

  • Acidithiobacillus thiooxidansのチオ硫酸デヒドロゲナーゼの遺伝子クローニング

    第55回日本生物工学会大会  

    発表年月: 2003年

  • Arabidopsis thalianaにおけるCBP2ホモログの組織特異的発現

    日本植物学会年会  

    発表年月: 2003年

  • 自家不和合性制御因子ARC1のU-BOX結合タンパク質のCDNAクローンの解析

    第21回日本植物細胞分子生物学会大会  

    発表年月: 2003年

  • Isolation of the ARC1 cDNA of Brassica campestris and cDNA screening for U-box binding proteins

    7th International Congress of Plant Molecular Biology  

    発表年月: 2003年

  • Expression and characterization of the recombinant thiocyanate hydrolase

    発表年月: 2003年

  • ブラシノステロイドレセプターキナーゼ(BRI1-KD)と相互作用するタンパク質の細胞内リン酸化の解析

    第25回日本分子生物学会年会  

    発表年月: 2002年

  • Brassica campestrisのARC1 cDNAの単離とU-box結合タンパク質のcDNAスクリーニング

    第25回日本分子生物学会年会  

    発表年月: 2002年

  • タバコモザイクウイルス抵抗性遺伝子Nの類似遺伝子のcDNA単離と選択的スプライシングの解析

    第25回日本分子生物学会年会  

    発表年月: 2002年

  • GFP遺伝子を利用したToMV移行蛋白質と宿主因子の相互作用の解析

    第25回日本分子生物学会年会  

    発表年月: 2002年

  • 核移行シグナル受容体Importinαを用いた核タンパク質cDNAの網羅的単離法の開発

    第25回日本分子生物学会年会  

    発表年月: 2002年

  • cDNA cloning of a cytokinin-binding protein (CBP2)

    17th International Conference on Plant Growth Substances  

    発表年月: 2001年

  • cDNA cloning for host factors interacting with the movement protein of tomato mosaic virus

    第15回韓国植物学会大会(基調講演)  

    発表年月: 2001年

  • Characterization of plant proteins interacting with the movement protein of tomato mosaic virus

    XIth International congress of virology  

    発表年月: 1999年

  • STRUCTURE OF THE GENE CODING FOR CARBAMYL-PHOSPHATE SYNTHETASE-I OF RAT

    丹生谷 博

    日本遺伝学会  

    発表年月: 1985年

  • DNA-SEQUENCE OF THE CARA GENE - TANDEM PROMOTERS, RESPECTIVELY CONTROLLED BY ARGININE AND THE PYRIMIDINES, REGULATE THE SYNTHESIS OF CARBAMOYLPHOSPHATE SYNTHETASE IN ESCHERICHIA-COLI-K-12

    J.Piette

    Archives Internationales De Physiologie De Biochimie Et De Biophysique  

    発表年月: 1983年

  • DNA-SEQUENCE OF THE CARB GENE OF ESCHERICHIA-COLI CODING FOR THE LARGE SUBUNIT OF CARBAMYL-PHOSPHATE SYNTHETASE

    C.J. Lusty

    Federation Proceedings  

    発表年月: 1983年

  • RELATIONSHIP BETWEEN FRUITING BODY FORMATION AND CARBOHYDRATE-METABOLISM IN A MUTANT (PGI) OF COPRINUS-MACRORHIZUS

    丹生谷 博

    日本遺伝学会  

    発表年月: 1980年

  • EFFECT OF PHOSPHOGLUCOSE ISOMERASE MUTATION ON FRUITING BODY FORMATION IN COPRINUS-MACRORHIZUS

    丹生谷 博

    日本遺伝学会  

    発表年月: 1979年

  • EFFECT OF 2-DEOXY-D-GLUCOSE ON FRUITING BODY FORMATION AND TYROSINASE PRODUCTION IN COPRINUS-MACRORHIZUS

    丹生谷 博

    日本遺伝学会  

    発表年月: 1977年

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