戸谷 美夏 (トヤ ミカ)

写真a

所属

理工学術院 国際理工学センター(理工学術院)

職名

准教授(任期付)

兼担 【 表示 / 非表示

  • 附属機関・学校   グローバルエデュケーションセンター

学内研究所等 【 表示 / 非表示

  • 2020年
    -
    2022年

    理工学術院総合研究所   兼任研究員

学位 【 表示 / 非表示

  • 東京大学   博士(理学)

所属学協会 【 表示 / 非表示

  •  
     
     

    日本細胞生物学会

  •  
     
     

    日本分子生物学会

 

研究分野 【 表示 / 非表示

  • 細胞生物学

研究キーワード 【 表示 / 非表示

  • 細胞構造・細胞形態と機能

  • 微小管

  • 細胞骨格

  • 上皮細胞

論文 【 表示 / 非表示

  • Patronin Takes a Shot at Polarity

    Masatoshi Takeichi, Mika Toya

    DEVELOPMENTAL CELL   38 ( 1 ) 12 - 13  2016年07月  [招待有り]

     概要を見る

    To specify the anterior-posterior axis of Drosophila embryos, noncentrosomal microtubules grow out from cortical regions of the oocyte and help transport axis determinants. In this issue of Developmental Cell, Nashchekin et al. (2016) report a Shot- and Patronin-dependent mechanism by which the oocyte cortex produces polarized microtubule arrays.

    DOI PubMed

  • CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells

    Mika Toya, Saeko Kobayashi, Miwa Kawasaki, Go Shioi, Mari Kaneko, Takashi Ishiuchi, Kazuyo Misaki, Wenxiang Meng, Masatoshi Takeichi

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 2 ) 332 - 337  2016年01月  [査読有り]

     概要を見る

    Polarized epithelial cells exhibit a characteristic array of microtubules that are oriented along the apicobasal axis of the cells. The minus-ends of these microtubules face apically, and the plus-ends face toward the basal side. The mechanisms underlying this epithelial-specific microtubule assembly remain unresolved, however. Here, using mouse intestinal cells and human Caco-2 cells, we show that the microtubule minus-end binding protein CAMSAP3 (calmodulin-regulated-spectrin-associated protein 3) plays a pivotal role in orienting the apical-to-basal polarity of microtubules in epithelial cells. In these cells, CAMSAP3 accumulated at the apical cortices, and tethered the longitudinal microtubules to these sites. Camsap3 mutation or depletion resulted in a random orientation of these microtubules; concomitantly, the stereotypic positioning of the nucleus and Golgi apparatus was perturbed. In contrast, the integrity of the plasma membrane was hardly affected, although its structural stability was decreased. Further analysis revealed that the CC1 domain of CAMSAP3 is crucial for its apical localization, and that forced mislocalization of CAMSAP3 disturbs the epithelial architecture. These findings demonstrate that apically localized CAMSAP3 determines the proper orientation of microtubules, and in turn that of organelles, in mature mammalian epithelial cells.

    DOI PubMed

  • Organization of Non-centrosomal Microtubules in Epithelial Cells

    Mika Toya, Masatoshi Takeichi

    CELL STRUCTURE AND FUNCTION   41 ( 2 ) 127 - 135  2016年  [査読有り]  [招待有り]

     概要を見る

    Polarized epithelial cells contain a characteristic array of microtubules in which non-centrosomal microtubules are aligned along the apical-to-basal axis of the cell with their minus ends oriented towards the apical pole. Although this unique orientation of microtubules was discovered in the late 1980s, how this orientation is established remains unresolved partly because of limited information about molecular factors that regulate the minus ends of non-centrosomal microtubules. Recent studies, however, identified novel minus end-associated proteins, revealing mechanisms by which the polarized arrays of microtubules are established in epithelial cells. These studies have also demonstrated the importance of apico-basally orientated microtubules in intra-structural organization of cells. This review focuses on recent progress of our understanding of the molecular basis for epithelium-specific microtubule assembly and function.

    DOI PubMed

  • The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

    Elsa Kress, Francoise Schwager, Rene Holtackers, Jonas Seiler, Francois Prodon, Esther Zanin, Annika Eiteneuer, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraldi, Monica Gotta

    JOURNAL OF CELL BIOLOGY   201 ( 4 ) 559 - 575  2013年05月  [査読有り]

     概要を見る

    Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

    DOI PubMed

  • The nucleoporin Nup205/NPP-3 is lost near centrosomes at mitotic onset and can modulate the timing of this process in Caenorhabditis elegans embryos

    Virginie Hachet, Coralie Busso, Mika Toya, Asako Sugimoto, Peter Askjaer, Pierre Goenczy

    MOLECULAR BIOLOGY OF THE CELL   23 ( 16 ) 3111 - 3121  2012年08月  [査読有り]

     概要を見る

    Regulation of mitosis in time and space is critical for proper cell division. We conducted an RNA interference-based modifier screen to identify novel regulators of mitosis in Caenorhabditis elegans embryos. Of particular interest, this screen revealed that the Nup205 nucleoporin NPP-3 can negatively modulate the timing of mitotic onset. Furthermore, we discovered that NPP-3 and nucleoporins that are associated with it are lost from the nuclear envelope (NE) in the vicinity of centrosomes at the onset of mitosis. We demonstrate that centrosomes are both necessary and sufficient for NPP-3 local loss, which also requires the activity of the Aurora-A kinase AIR-1. Our findings taken together support a model in which centrosomes and AIR-1 promote timely onset of mitosis by locally removing NPP-3 and associated nucleoporins from the NE.

    DOI PubMed

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Misc 【 表示 / 非表示

  • Identification and functional analysis of the gene for Class I myosin in S-pombe.

    M Toya, F Motegi, K Nakano, Mabuchi, I, M Yamamoto

    YEAST   18   S208 - S208  2001年08月

    研究発表ペーパー・要旨(国際会議)  

特定課題研究 【 表示 / 非表示

  • 非中心体微小管が関わる腎尿細管構造の生理的役割

    2019年   佐藤政充, 竹市雅俊

     概要を見る

    腎臓は、体内の水分や塩分を調節する重要な役割をもつ。その構造は複雑で、皮質と髄質に分けられる2つの層に、糸球体と尿細管から成るネフロンと呼ばれる最小機能単位が、多数詰まっている。我々は、中心体に依存しない微小管に結合するCAMSAP3タンパク質の機能欠損マウスにおいて、腎臓の構造異常を見出した。組織化学解析から、Camsap3変異マウスでは、腎皮質に存在する尿細管の一部が拡張することがわかった。本研究課題では、尿および血液サンプルの生化学検査をおこない、CAMSAP3の機能欠損により生じる腎臓の構造異常が、生理的機能に与える影響を調べた。

  • 上皮細胞に特徴的な微小管編成が構築されるしくみ

    2018年  

     概要を見る

    上皮細胞は、臓器を構成する主要な成分であり、生体内で頂端―基底の極性をもつ。分化した上皮細胞では、微小管細胞骨格は、頂端―基底の軸に沿って規則正しく配向する。この特徴的な微小管編成は、上皮細胞が正しく機能するために重要であると考えられているが、編成構築のしくみについては、いまだ多くのことが分かっていない。本研究では、幹細胞領域と分化した細胞を同時に観察することが可能な、マウス小腸の上皮組織に着目した。絨毛に隣接して窪んだ、上皮幹細胞を含む領域(陰窩)と、分化した細胞から成る絨毛中央部に存在する細胞について、微小管編成を比較観察し、分化にともなう微小管編成の変化を調べた。

 

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