2022/05/26 更新

写真a

ソノイケ キンタケ
園池 公毅
所属
教育・総合科学学術院 教育学部
職名
教授
ホームページ
プロフィール
昭和36年東京生まれ。東京大学教養学部卒。東京大学大学院理学系研究科博士課程修了。理学博士。理化学研究所特別研究生、東京大学理学部助手、東京大学新領域創成科学研究科准教授を経て、早稲田大学教育・総合科学学術院教授。専門は植物生理学、特に光合成。
植物の光合成について主に生理学の視点から研究を進めるとともに、サイト「光合成の森」などにより、一般向けに光合成の紹介を行なっている。また、和歌の披講や装束の衣紋といった伝統文化の継承にも携わり、新年の宮中行事の一つである歌会始の儀の披講所役を30年余にわたって務めている。

兼担

  • 理工学術院   大学院先進理工学研究科

  • 附属機関・学校   グローバルエデュケーションセンター

学内研究所等

  • 2020年
    -
    2022年

    理工学術院総合研究所   兼任研究員

学歴

  • 1985年04月
    -
    1998年03月

    東京大学   大学院理学系研究科   相関理化学専攻博士課程  

  • 1983年04月
    -
    1985年03月

    東京大学   大学院理学系研究科   相関理化学専攻修士課程  

  • 1979年04月
    -
    1983年03月

    東京大学   教養学部   基礎科学科  

学位

  • 東京大学   理学博士

経歴

  • 2009年09月
    -
    継続中

    早稲田大学   教育・総合科学学術院   教授

  • 1983年07月
    -
    継続中

    宮内庁   式部職   嘱託

  • 1999年04月
    -
    2009年09月

    東京大学   大学院新領域創成科学研究科   助教授(准教授)

  • 1990年04月
    -
    1999年03月

    東京大学   理学部   助手

  • 1989年04月
    -
    1990年03月

    理化学研究所   太陽光エネルギーグループ   特別研究生

  • 1988年04月
    -
    1989年03月

    東京大学   大学院理学系研究科   大学院研究生

▼全件表示

所属学協会

  •  
     
     

    国際光合成学会

  •  
     
     

    アメリカ植物科学会

  •  
     
     

    日本植物生理学会

  •  
     
     

    日本植物学会

  •  
     
     

    International Society of Photosynthesis Research

  •  
     
     

    日本光合成学会

▼全件表示

 

研究分野

  • 植物分子、生理科学   光合成

研究キーワード

  • 光合成

  • photosynthesis

論文

  • Investigation of Nostoc sp. HK-01, Cell Survival over Three Years during the Tanpopo Mission

    Kaori Tomita-Yokotani, Shunta Kimura, Midori Ong, Miku Tokita, Hiroshi Katoh, Tomoko Abe, Hirofumi Hashimoto, Kintake Sonoike, Masayuki Ohmori

    Astrobiology   21 ( 12 ) 1505 - 1514  2021年12月  [査読有り]

    DOI

  • Respiration Interacts With Photosynthesis Through the Acceptor Side of Photosystem I, Reflected in the Dark-to-Light Induction Kinetics of Chlorophyll Fluorescence in the Cyanobacterium Synechocystis sp. PCC 6803

    Takako Ogawa, Kenta Suzuki, Kintake Sonoike

    Frontiers in Plant Science   12   717968  2021年07月  [査読有り]  [招待有り]

    担当区分:最終著者, 責任著者

    DOI

  • Imaging, screening and remote sensing of photosynthetic activity and stress responses.

    Kaori Kohzuma, Kintake Sonoike, Kouki Hikosaka

    Journal of plant research   134 ( 4 ) 649 - 651  2021年07月  [国内誌]

    DOI PubMed

  • Screening of mutants using chlorophyll fluorescence

    Takako Ogawa, Kintake Sonoike

    Journal of Plant Research   134   653 - 664  2021年03月  [査読有り]  [招待有り]

    担当区分:最終著者, 責任著者

    DOI

  • Dissection of the mechanisms of growth inhibition resulting from loss of the PII protein in the cyanobacterium Synechococcus elongatus PCC 7942.

    Takayuki Sakamoto, Nobuyuki Takatani, Kintake Sonoike, Haruhiko Jimbo, Yoshitaka Nishiyama, Tatsuo Omata

    Plant & cell physiology   62   721 - 731  2021年02月  [査読有り]  [国内誌]

     概要を見る

    In cyanobacteria, the PII protein (the glnB gene product) regulates a number of proteins involved in nitrogen assimilation including PipX, the co-activator of the global nitrogen regulator protein NtcA. In Synechococcus elongatus PCC 7942, construction of a PII-less mutant retaining the wild-type pipX gene is difficult because of the toxicity of uncontrolled action of PipX and the other defect(s) resulting from the loss of PII  per se, but the nature of the PipX toxicity and the PipX-independent defect(s) remains unclear. Characterization of a PipX-less glnB mutant (PD4) in this study showed that the loss of PII increases the sensitivity of PSII to ammonium. Ammonium was shown to stimulate formation of reactive oxygen species in the mutant cells. The ammonium-sensitive growth phenotype of PD4 was rescued by addition of an antioxidant α-tocopherol, confirming that photo-oxidative damage was the major cause of the growth defect. A targeted PII mutant retaining wild-type pipX was successfully constructed from the wild-type S. elongatus strain (SPc) in the presence of α-tocopherol. The resulting mutant (PD1X) showed an unusual chlorophyll fluorescence profile, indicating extremely slow reduction and re-oxidation of QA, which phenotype was not observed in the mutant defective in both glnB and pipX. These results showed that the aberrant action of uncontrolled PipX resulted in impairment of the electron transport reactions in both the reducing and oxidizing sides of QA.

    DOI PubMed

  • The NAD kinase Slr0400 functions as a growth repressor in Synechocystis sp. PCC 6803.

    Yuuma Ishikawa, Cedric Cassan, Aikeranmu Kadeer, Koki Yuasa, Nozomu Sato, Kintake Sonoike, Yasuko Kaneko, Atsuko Miyagi, Hiroko Takahashi, Toshiki Ishikawa, Masatoshi Yamaguchi, Yoshitaka Nishiyama, Yukako Hihara, Yves Gibon, Maki Kawai-Yamada

    Plant & cell physiology   62   668 - 677  2021年02月  [査読有り]  [国内誌]

     概要を見る

    NADP+, the phosphorylated form of nicotinamide adenine dinucleotide (NAD), plays an essential role in many cellular processes. NAD kinase (NADK), which is conserved in all living organisms, catalyzes the phosphorylation of NAD+ to NADP+. However, the physiological role of phosphorylation of NAD+ to NADP+ in the cyanobacterium Synechocystis remains unclear. In this study, we report that slr0400, an NADK-encoding gene in Synechocystis, functions as a growth repressor under light-activated heterotrophic growth conditions and light and dark cycle conditions in the presence of glucose. We show, via characterization of NAD(P)(H) content and enzyme activity, that NAD+ accumulation in slr0400-deficient mutant results in unsuppressed activity of glycolysis and tricarboxylic acid (TCA) cycle enzymes. In determining whether Slr0400 functions as a typical NADK, we found that constitutive expression of slr0400 in an Arabidopsis nadk2-mutant background complements the pale-green phenotype. Moreover, to determine the physiological background behind the growth advantage of mutants lacking slr04000, we investigated the photobleaching phenotype of slr0400-deficient mutant under high-light conditions. Photosynthetic analysis found in the slr0400-deficient mutant resulted from malfunctions in the PSII photosynthetic machinery. Overall, our results suggest that NADP(H)/NAD(H) maintenance by slr0400 plays a significant role in modulating glycolysis and the TCA cycle to repress the growth rate and maintain photosynthetic capacity.

    DOI PubMed

  • Morphological and cytological observations of corolla green spots reveal the presence of functional chloroplasts in Japanese gentian.

    Shigekazu Takahashi, Suguru Ozawa, Kintake Sonoike, Katsutomo Sasaki, Masahiro Nishihara

    PloS one   15 ( 8 ) e0237173  2020年08月  [査読有り]  [国際誌]

    DOI PubMed

  • Light dependent accumulation of β-carotene enhances photo-acclimation of Euglena gracilis

    Yuri Tanno, Shota Kato, Senji Takahashi, Shun Tamaki, Shinichi Takaichi, Yutaka Kodama, Kintake Sonoike, Tomoko Shinomura

    Journal of Photochemistry and Photobiology B: Biology   209   111950  2020年08月  [査読有り]

    DOI

  • Direct injection of pigment–protein complexes and membrane fragments suspended in water from phototrophs to C18 HPLC

    Shinichi Takaichi, Akira Okoshi, Seiu Otomo, Masahiro Misumi, Kintake Sonoike, Jiro Harada

      144 ( 1 ) 101 - 107  2020年04月  [査読有り]

    DOI

  • Guard cell photosynthesis is crucial in abscisic acid-induced stomatal closure

    Iwai, S, Ogata, S, Yamada, N, Onjo, M, Sonoike, K, Shimazaki, K

    Plant Direct   3 ( 5 ) e00137  2019年06月  [査読有り]

    DOI

  • Crassulacean acid metabolism induction in Mesembryanthemum crystallinum can be estimated by non-photochemical quenching upon actinic illumination during the dark period.

    Matsuoka, T, Onozawa, A, Sonoike, K, Kore-eda, S

    Plant and Cell Physiology   59   1966 - 1975  2018年10月  [査読有り]

    DOI

  • Evaluation of the condition of respiration and photosynthesis by measuring chlorophyll fluorescence in cyanobacteria.

    Ogawa, T, Sonoike, K

    BioProtocol   8 ( 9 ) e2834  2018年05月  [査読有り]

    DOI

  • Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence: problems and solutions

    Takako Ogawa, Masahiro Misumi, Kintake Sonoike

    PHOTOSYNTHESIS RESEARCH   133 ( 1-3 ) 63 - 73  2017年09月  [査読有り]

     概要を見る

    Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, F (v)/F (m), some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.

    DOI

  • Significance of structural variation in thylakoid membranes in maintaining functional photosystems during reproductive growth

    Hatsumi Nozue, Kaori Oono, Yoshinobu Ichikawa, Shun Tanimura, Kana Shirai, Kintake Sonoike, Masayuki Nozue, Nobuaki Hayashida

    PHYSIOLOGIA PLANTARUM   160 ( 1 ) 111 - 123  2017年05月  [査読有り]

     概要を見る

    Structural variation in the stroma-grana (SG) arrangement of the thylakoid membranes, such as changes in the thickness of the grana stacks and in the ratio between grana and inter-grana thylakoid, is often observed. Broadly, such alterations are considered acclimation to changes in growth and the environment. However, the relation of thylakoid morphology to plant growth and photosynthesis remains obscure. Here, we report changes in the thylakoid during leaf development under a fixed light condition. Histological studies on the chloroplasts of fresh green Arabidopsis leaves have shown that characteristically shaped thylakoid membranes lacking the inter-grana region, referred to hereafter as isolated-grana (IG), occurred adjacent to highly ordered, large grana layers. This morphology was restored to conventional SG thylakoid membranes with the removal of bolting stems from reproductive plants. Statistical analysis showed a negative correlation between the incidences of IG-type chloroplasts in mesophyll cells and the rates of leaf growth. Fluorescence parameters calculated from pulse-amplitude modulated fluorometry measurements and CO2 assimilation data showed that the IG thylakoids had a photosynthetic ability that was equivalent to that of the SG thylakoids under moderate light. However, clear differences were observed in the chlorophyll a/b ratio. The IG thylakoids were apparently an acclimated phenotype to the internal condition of source leaves. The idea is supported by the fact that the life span of the IG thylakoids increased significantly in the later developing leaves. In conclusion, the heterogeneous state of thylakoid membranes is likely important in maintaining photosynthesis during the reproductive phase of growth.

    DOI

  • Characterization of the influence of chlororespiration on the regulation of photosynthesis in the glaucophyte Cyanophora paradoxa

    Masahiro Misumi, Kintake Sonoike

    SCIENTIFIC REPORTS   7   46100  2017年04月  [査読有り]

     概要を見る

    Glaucophytes are primary symbiotic algae with unique plastids called cyanelles, whose structure is most similar to ancestral cyanobacteria among plastids in photosynthetic organisms. Here we compare the regulation of photosynthesis in glaucophyte with that in cyanobacteria in the aim of elucidating the changes caused by the symbiosis in the interaction between photosynthetic electron transfer and other metabolic pathways. Chlorophyll fluorescence measurements of the glaucophyte Cyanophora paradoxa NIES-547 indicated that plastoquinone (PQ) pool in photosynthetic electron transfer was reduced in the dark by chlororespiration. The levels of nonphotochemical quenching of chlorophyll fluorescence was high in the dark but decreased under low light, and increased again under high light. This type of concave light dependence was quite similar to that observed in cyanobacteria. Moreover, the addition of ionophore hardly affected nonphotochemical quenching, suggesting state transition as a main component of the regulatory system in C. paradoxa. These results suggest that cyanelles of C. paradoxa retain many of the characteristics observed in their ancestral cyanobacteria. From the viewpoint of metabolic interactions, C. paradoxa is the primary symbiotic algae most similar to cyanobacteria than other lineages of photosynthetic organisms.

    DOI

  • Relationship Between Photochemical Quenching and Non-Photochemical Quenching in Six Species of Cyanobacteria Reveals Species Difference in Redox State and Species Commonality in Energy Dissipation

    Masahiro Misumi, Hiroshi Katoh, Tatsuya Tomo, Kintake Sonoike

    PLANT AND CELL PHYSIOLOGY   57 ( 7 ) 1510 - 1517  2016年07月  [査読有り]

     概要を見る

    Although the photosynthetic reaction center is well conserved among different cyanobacterial species, the modes of metabolism, e.g. respiratory, nitrogen and carbon metabolism and their mutual interaction, are quite diverse. To explore such uniformity and diversity among cyanobacteria, here we compare the influence of the light environment on the condition of photosynthetic electron transport through Chl fluorescence measurement of six cyanobacterial species grown under the same photon flux densities and at the same temperature. In the dark or under weak light, up to growth light, a large difference in the plastoquinone (PQ) redox condition was observed among different cyanobacterial species. The observed difference indicates that the degree of interaction between respiratory electron transfer and photosynthetic electron transfer differs among different cyanobacterial species. The variation could not be ascribed to the phylogenetic differences but possibly to the light environment of the original habitat. On the other hand, changes in the redox condition of PQ were essentially identical among different species at photon flux densities higher than the growth light. We further analyzed the response to high light by using a typical energy allocation model and found that 'non-regulated' thermal dissipation was increased under high-light conditions in all cyanobacterial species tested. We assume that such 'non-regulated' thermal dissipation may be an important 'regulatory' mechanism in the acclimation of cyanobacterial cells to high-light conditions.

    DOI PubMed

  • Effects of Bleaching by Nitrogen Deficiency on the Quantum Yield of Photosystem II in Synechocystis sp PCC 6803 Revealed by Chl Fluorescence Measurements

    Takako Ogawa, Kintake Sonoike

    PLANT AND CELL PHYSIOLOGY   57 ( 3 ) 558 - 567  2016年03月  [査読有り]

     概要を見る

    Estimation of photosynthesis by Chl fluorescence measurement of cyanobacteria is always problematic due to the interference from respiratory electron transfer and from phycocyanin fluorescence. The interference from respiratory electron transfer could be avoided by the use of DCMU or background illumination by blue light, which oxidizes the plastoquinone pool that tends to be reduced by respiration. On the other hand, the precise estimation of photosynthesis in cells with a different phycobilisome content by Chl fluorescence measurement is difficult. By subtracting the basal fluorescence due to the phycobilisome and PSI, it becomes possible to estimate the precise maximum quantum yield of PSII in cyanobacteria. Estimated basal fluorescence accounted for 60% of the minimum fluorescence, resulting in a large difference between the 'apparent' yield and 'true' yield under high phycocyanin conditions. The calculated value of the 'true' maximum quantum yield of PSII was around 0.8, which was similar to the value observed in land plants. The results suggest that the cause of the apparent low yield reported in cyanobacteria is mainly ascribed to the interference from phycocyanin fluorescence. We also found that the 'true' maximum quantum yield of PSII decreased under nitrogen-deficient conditions, suggesting the impairment of the PSII reaction center, while the 'apparent' maximum quantum yield showed a marginal change under the same conditions. Due to the high contribution of phycocyanin fluorescence in cyanobacteria, it is essential to eliminate the influence of the change in phycocyanin content on Chl fluorescence measurement and to evaluate the 'true' photosynthetic condition.

    DOI

  • Analysis of spontaneous suppressor mutants from the photomixotrophically grown pmgA-disrupted mutant in the cyanobacterium Synechocystis sp PCC 6803

    Yoshiki Nishijima, Yu Kanesaki, Hirofumi Yoshikawa, Takako Ogawa, Kintake Sonoike, Yoshitaka Nishiyama, Yukako Hihara

    PHOTOSYNTHESIS RESEARCH   126 ( 2-3 ) 465 - 475  2015年12月  [査読有り]

     概要を見る

    The pmgA-disrupted (Delta pmgA) mutant in the cyanobacterium Synechocystis sp. PCC 6803 suffers severe growth inhibition under photomixotrophic conditions. In order to elucidate the key factors enabling the cells to grow under photomixotrophic conditions, we isolated spontaneous suppressor mutants from the Delta pmgA mutant derived from a single colony. When the Delta pmgA mutant was spread on a BG11 agar plate supplemented with glucose, colonies of suppressor mutants appeared after the bleaching of the background cells. We identified the mutation site of these suppressor mutants and found that 11 mutants out of 13 had a mutation in genes related to the type 1 NAD(P)H dehydrogenase (NDH-1) complex. Among them, eight mutants had mutations within the ndhF3 (sll1732) gene: R32stop, W62stop, V147I, G266V, G354W, G586C, and deletion of 7 bp within the coding region. One mutant had one base insertion in the putative -10 box of the ndhC (slr1279) gene, leading to the decrease in the transcripts of the ndhCKJ operon. Two mutants had one base insertion and deletion in the coding region of cupA (sll1734), which is co-transcribed with ndhF3 and ndhD3 and comprises together a form of NDH-1 complex (NDH-1MS complex) involved in inducible high-affinity CO2 uptake. The results indicate that the loss of the activity of this complex effectively rescues the Delta pmgA mutant under photomixotrophic condition with 1 % CO2. However, little difference among WT and mutants was observed in the activities ascribed to the NDH-1MS complex, i.e., CO2 uptake and cyclic electron transport. This may suggest that the NDH-1MS complex has the third, currently unknown function under photomixotrophic conditions.

    DOI

  • The initiation of nocturnal dormancy in Synechococcus as an active process

    Sotaro Takano, Jun Tomita, Kintake Sonoike, Hideo Iwasaki

    BMC BIOLOGY   13   36  2015年06月  [査読有り]

     概要を見る

    Background: Most organisms, especially photoautotrophs, alter their behaviours in response to day-night alternations adaptively because of their great reliance on light. Upon light-to-dark transition, dramatic and universal decreases in transcription level of the majority of the genes in the genome of the unicellular cyanobacterium, Synechococcus elongatus PCC 7942 are observed. Because Synechococcus is an obligate photoautotroph, it has been generally assumed that repression of the transcription in the dark (dark repression) would be caused by a nocturnal decrease in photosynthetic activities through the reduced availability of energy (e.g. adenosine triphosphate (ATP)) needed for mRNA synthesis.
    Results: However, against this general assumption, we obtained evidence that the rapid and dynamic dark repression is an active process. Although the addition of photosynthesis inhibitors to cells exposed to light mimicked transcription profiles in the dark, it did not significantly affect the cellular level of ATP. By contrast, when ATP levels were decreased by the inhibition of both photosynthesis and respiration, the transcriptional repression was attenuated through inhibition of RNA degradation. This observation indicates that Synechococcus actively downregulates genome-wide transcription in the dark. Even though the level of total mRNA dramatically decreased in the dark, Synechococcus cells were still viable, and they do not need de novo transcription for their survival in the dark for at least 48 hours.
    Conclusions: Dark repression appears to enable cells to enter into nocturnal dormancy as a feed-forward process, which would be advantageous for their survival under periodic nocturnal conditions.

    DOI

  • Zeaxanthin and Echinenone Protect the Repair of Photosystem II from Inhibition by Singlet Oxygen in Synechocystis sp PCC 6803

    Yuri Kusama, Shuhei Inoue, Haruhiko Jimbo, Shinichi Takaichi, Kintake Sonoike, Yukako Hihara, Yoshitaka Nishiyama

    PLANT AND CELL PHYSIOLOGY   56 ( 5 ) 906 - 916  2015年05月  [査読有り]

     概要を見る

    Carotenoids are important components of antioxidative systems in photosynthetic organisms. We investigated the roles of zeaxanthin and echinenone in the protection of PSII from photoinhibition in Synechocystis sp. PCC 6803, using mutants of the cyanobacterium that lack these carotenoids. The activity of PSII in mutant cells deficient in either zeaxanthin or echinenone was more sensitive to strong light than the activity in wild-type cells, and the activity in mutant cells deficient in both carotenoids was hypersensitive to strong light, indicating that the absence of these carotenoids increased the extent of photoinhibition. Nonetheless, the rate of photodamage to PSII, as measured in the presence of chloramphenicol, which blocks the repair of PSII, was unaffected by the absence of either carotenoid, suggesting that these carotenoids might act by protecting the repair of PSII. Knockout of the gene for the so-called orange carotenoid protein (OCP), in which the 3'-hydroxyechinenone cofactor, a derivative of echinenone, is responsible for the thermal dissipation of excitation energy, increased the extent of photoinhibition but did not affect photodamage, suggesting that thermal dissipation also protects the repair of PSII. In mutant cells lacking OCP, as well as those lacking zeaxanthin and echinenone, the production of singlet oxygen was stimulated and the synthesis de novo of various proteins, including the D1 protein, was markedly suppressed under strong light. These observations suggest that the carotenoids and thermal dissipation might protect the repair of photodamaged PSII by depressing the levels of singlet oxygen that inhibits protein synthesis.

    DOI

  • Dissection of respiration and photosynthesis in the cyanobacterium Synechocystis sp PCC6803 by the analysis of chlorophyll fluorescence

    Takako Ogawa, Kintake Sonoike

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   144   61 - 67  2015年03月  [査読有り]

     概要を見る

    In cyanobacteria, photosynthesis and respiration share some components of electron transport chain. To explore the interaction between photosynthesis and respiration, we monitored the change in the yield of chlorophyll fluorescence due to state transition in ndh genes disruptants, deficient in NAD(P)H dehydrogenase (NDH-1) complexes serving for respiration or for carbon concentrating mechanism (CCM). The disruption of ndh genes essential for respiration resulted in low levels of chlorophyll fluorescence quenching in the dark (NPQ(Dark)) as well as in the low light (NPQ(LL)). The lowered NPQ(Dark) and NPQ(LL) in these ndh genes disruptants could be ascribed to the oxidation of the PQ pool due to the poor electron supply from NDH-1 complexes in respiratory electron transport. On the other hand, only NPQ(LL) decreased upon disruption of the ndh genes essential for CCM. We propose that, in the disruptants of these ndh genes, the PQ pool is oxidized in the light through the increased photosystem I content, resulting in the lowered NPQ(LL). Apparently, the two different subsets of ndh genes affect photosynthetic electron transport although in totally different manners. It is also suggested that monitoring state transition is a simple method to evaluate the condition of photosynthesis, respiration and CCM. (C) 2015 Elsevier B.V. All rights reserved.

    DOI

  • The Heat Tolerance of Dry Colonies of a Terrestrial Cyanobacterium, Nostoc sp. HK-01

    Shunta Kimura, Kaori Tomita-Yokotani, Yuichi Igarashi, Seigo Sato, Hiroshi Katoh, Tomoko Abe, Kintake Sonoike, Masayuki Ohmori

    Biological Sciences in Space   29 ( 0 ) 12 - 18  2015年  [査読有り]

    DOI

  • Fe deficiency induces phosphorylation and translocation of Lhcb1 in barley thylakoid membranes

    Akihiro Saito, Mizuho Shimizu, Hitomi Nakamura, Shoko Maeno, Riko Katase, Eitaro Miwa, Kyoko Higuchi, Kintake Sonoike

    FEBS LETTERS   588 ( 12 ) 2042 - 2048  2014年06月  [査読有り]

     概要を見る

    HvLhcb1 a major light-harvesting chlorophyll a/b-binding protein in barley, is a critical player in sustainable growth under Fe deficiency. Here, we demonstrate that Fe deficiency induces phosphorylation of HvLhcb1 proteins leading to their migration from grana stacks to stroma thylakoid membranes. HvLhcbl remained phosphorylated even in the dark and apparently independently of state transition, which represents a mechanism for short-term acclimation. Our data suggest that the constitutive phosphorylation-triggered translocation of HvLhcbl under Fe deficiency contributes to optimization of the excitation balance between photosystem II and photosystem I, the latter of which is a main target of Fe deficiency. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI

  • Disruption of the ndhF1 gene affects Chl fluorescence through state transition in the cyanobacterium synechocystis sp. PCC 6803, resulting in apparent high efficiency of photosynthesis

    Takako Ogawa, Tetsuyuki Harada, Hiroshi Ozaki, Kintake Sonoike

    Plant and Cell Physiology   54 ( 7 ) 1164 - 1171  2013年07月  [査読有り]

     概要を見る

    In Synechocystis sp. PCC 6803, the disruption of the ndhF1 gene (slr0844), which encodes a subunit of one of the NDH-1 complexes (NDH-1L complex) serving for respiratory electron transfer, causes the largest change in Chl fluorescence induction kinetics among the kinetics of 750 disruptants searched in the Fluorome, the cyanobacterial Chl fluorescence database. The cause of the explicit phenotype of the ndhF1 disruptant was examined by measurements of the photosynthetic rate, Chl fluorescence and state transition. The results demonstrate that the defects in respiratory electron transfer obviously have great impact on Chl fluorescence in cyanobacteria. The inactivation of NDH-1L complexes involving electron transfer from NDH-1 to plastoquinone (PQ) would result in the oxidation of the PQ pool, leading to the transition to State 1, where the yield of Chl fluorescence is high. Apparently, respiration, although its rate is far lower than that of photosynthesis, could affect Chl fluorescence through the state transition as leverage. The disruption of the ndhF1 gene caused lower oxygen-evolving activity but the estimated electron transport rate from Chl fluorescence measurements was faster in the mutant than in the wild-type cells. The discrepancy could be ascribed to the decreased level of non-photochemical quenching due to state transition. One must be cautious when using the Chl fluorescence parameter to estimate photosynthesis in mutants defective in state transition. © 2013 The Author.

    DOI PubMed

  • Role of galactolipid biosynthesis in coordinated development of photosynthetic complexes and thylakoid membranes during chloroplast biogenesis in Arabidopsis

    Koichi Kobayashi, Takafumi Narise, Kintake Sonoike, Haruki Hashimoto, Naoki Sato, Maki Kondo, Mikio Nishimura, Mayuko Sato, Kiminori Toyooka, Keiko Sugimoto, Hajime Wada, Tatsuru Masuda, Hiroyuki Ohta

    PLANT JOURNAL   73 ( 2 ) 250 - 261  2013年01月  [査読有り]

     概要を見る

    The galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the predominant lipids in thylakoid membranes and indispensable for photosynthesis. Among the three isoforms that catalyze MGDG synthesis in Arabidopsis thaliana, MGD1 is responsible for most galactolipid synthesis in chloroplasts, whereas MGD2 and MGD3 are required for DGDG accumulation during phosphate (Pi) starvation. A null mutant of Arabidopsis MGD1 (mgd1-2), which lacks both galactolipids and shows a severe defect in chloroplast biogenesis under nutrient-sufficient conditions, accumulated large amounts of DGDG, with a strong induction of MGD2/3 expression, during Pi starvation. In plastids of Pi-starved mgd1-2 leaves, biogenesis of thylakoid-like internal membranes, occasionally associated with invagination of the inner envelope, was observed, together with chlorophyll accumulation. Moreover, the mutant accumulated photosynthetic membrane proteins upon Pi starvation, indicating a compensation for MGD1 deficiency by Pi stress-induced galactolipid biosynthesis. However, photosynthetic activity in the mutant was still abolished, and light-harvesting/ photosystem core complexes were improperly formed, suggesting a requirement for MGDG for proper assembly of these complexes. During Pi starvation, distribution of plastid nucleoids changed concomitantly with internal membrane biogenesis in the mgd1-2 mutant. Moreover, the reduced expression of nuclear-and plastid-encoded photosynthetic genes observed in the mgd1-2 mutant under Pi-sufficient conditions was restored after Pi starvation. In contrast, Pi starvation had no such positive effects in mutants lacking chlorophyll biosynthesis. These observations demonstrate that galactolipid biosynthesis and subsequent membrane biogenesis inside the plastid strongly influence nucleoid distribution and the expression of both plastid- and nuclear-encoded photosynthetic genes, independently of photosynthesis.

    DOI

  • Functional Analysis of Two Isoforms of Leaf-Type Ferredoxin-NADP(+)-Oxidoreductase in Rice Using the Heterologous Expression System of Arabidopsis

    Mieko Higuchi-Takeuchi, Takanari Ichikawa, Youichi Kondou, Keiko Matsui, Yukako Hasegawa, Mika Kawashima, Kintake Sonoike, Masaki Mori, Hirohiko Hirochika, Minami Matsui

    PLANT PHYSIOLOGY   157 ( 1 ) 96 - 108  2011年09月  [査読有り]

     概要を見る

    Ferredoxin-NADP(+)-oxidoreductase (FNR) mediates electron transfer between ferredoxin (Fd) and NADP(+); therefore, it is a key enzyme that provides the reducing power used in the Calvin cycle. Other than FNR, nitrite reductase, sulfite reductase, glutamate synthase, and Fd-thioredoxin reductase also accept electrons from Fd, an electron carrier protein in the stroma. Therefore, the regulation of electron partitioning in the chloroplast is important for photosynthesis and other metabolic pathways. The regulatory mechanism of electron partitioning, however, remains to be elucidated. We found, by taking advantage of a gain-of-function approach, that expression of two rice (Oryza sativa) full-length cDNAs of leaf-type FNRs (OsLFNR1 and OsLFNR2) led to altered chlorophyll fluorescence and growth in Arabidopsis (Arabidopsis thaliana) and rice. We revealed that overexpression of the OsLFNR1 and OsLFNR2 full-length cDNAs resulted in distinct phenotypes despite the high sequence similarity between them. Expression of OsLFNR1 affected the nitrogen assimilation pathway without inhibition of photosynthesis under normal conditions. On the other hand, OsLFNR2 expression led to the impairment of photosynthetic linear electron transport as well as Fd-dependent cyclic electron flow around photosystem I. The endogenous protein level of OsLFNR was found to be suppressed in both OsLFNR1-and OsLFNR2-overexpressing rice plants, leading to changes in the stoichiometry of the two LFNR isoforms within the thylakoid and soluble fractions. Thus, we propose that the stoichiometry of two LFNR isoforms plays an important role in electron partitioning between carbon fixation and nitrogen assimilation.

    DOI

  • Photoinhibition of photosystem I

    Kintake Sonoike

    PHYSIOLOGIA PLANTARUM   142 ( 1 ) 56 - 64  2011年05月  [査読有り]  [招待有り]

     概要を見る

    The photoinhibition of Photosystem I (PSI) drew less attention compared with that of Photosystem II (PSII). This could be ascribed to several reasons, e.g. limited combinations of plant species and environmental conditions that cause PSI photoinhibition, the non-regulatory aspect of PSI photoinhibition, and methodological difficulty to determine the accurate activity of PSI under stress conditions. However, the photoinhibition of PSI could be more dangerous than that of PSII because of the very slow recovery rate of PSI. This article is intended to introduce such characteristics of PSI photoinhibition with special emphasis on the relationship between two photosystems as well as the protective mechanism of PSI in vivo. Although the photoinhibition of PSI could be induced only in specific conditions and specific plant species in intact leaves, PSI itself is quite susceptible to photoinhibition in isolated thylakoid membranes. PSI seems to be well protected from photoinhibition in vivo in many plant species and many environmental conditions. This is quite understandable because photoinhibition of PSI is not only irreversible but also the potential cause of many secondary damages. This point would be different from the case of PSII photoinhibition, which could be regarded as one of the regulatory mechanisms under stressed as well as non-stressed conditions.

    DOI

  • Remodeling of the Major Light-Harvesting Antenna Protein of PSII Protects the Young Leaves of Barley (Hordeum vulgare L.) from Photoinhibition under Prolonged Iron Deficiency

    Akihiro Saito, Tomohisa Iino, Kintake Sonoike, Eitaro Miwa, Kyoko Higuchi

    PLANT AND CELL PHYSIOLOGY   51 ( 12 ) 2013 - 2030  2010年12月  [査読有り]

     概要を見る

    Because of the high demand for iron of the photosynthetic apparatus in thylakoid membranes, iron deficiency primarily affects the electron transfer between the two photosystems (PSI and PSII), resulting in photooxidative damage in plants. However, in barley, PSII is protected against photoinhibition, and the plant survives even with a low iron content in its chlorotic leaves. In this study, we report an adaptation mechanism of the photosynthetic apparatus in barley to iron deficiency, which is concomitant with the remodeling of a PSII antenna system. Transcriptome analysis revealed that long-term iron deficiency induced the expression of two genes of the major light-harvesting Chl a/b-binding protein of PSII (LHCII), namely HvLhcb1.11 and HvLhcb1.12. Chl fluorescence analysis of the wild type and Lhcb1-less chlorina mutants clearly showed that non-photochemical quenching (NPQ) of the wild type was increased by approximately 200% by iron deficiency, whereas NPQ of chlorina mutants did not change significantly under iron deficiency. The mutant showed severe photodamage in young leaves under prolonged iron deficiency, suggesting that the HvLhcb1 protein is essential for both thermal dissipation and photoprotection in iron-deficient barley. Analysis of thylakoid protein complexes revealed that the proportion of the monomeric form of Lhcb1 significantly increased in barley grown under iron-deficient conditions. We hypothesize that alteration of the HvLhcb1 subpopulations modifies the organization of LHCII in the thylakoid membranes, which is a key step for thermal dissipation to compensate for excess excitation energy and thereby protect the photosystems from serious damage in iron-deficient barley leaves.

    DOI PubMed CiNii

  • Quantitative analysis of the relationship between induction kinetics of chlorophyll fluorescence and function of genes in the cyanobacterium Synechocystis sp PCC 6803

    Hiroshi Ozaki, Kintake Sonoike

    PHOTOSYNTHESIS RESEARCH   101 ( 1 ) 47 - 58  2009年07月  [査読有り]

     概要を見る

    We developed here the quantitative and objective method to analyze chlorophyll fluorescence from the cyanobacterium Synechocystis sp. PCC 6803 in the aim of systematic examination of gene function. The overall similarity of the chlorophyll fluorescence induction kinetics was evaluated for 499 mutants. Mutants of 333 genes showed the difference in the fluorescence kinetics from that of wild type, indicating the wide interaction of photosynthesis with other metabolisms. Hierarchical clustering of the similarity of the mutants enables us to group together the mutants having defect in the regulation of photosystem stoichiometry as well as those having defects in respiration or other functions. Furthermore, wild-type cells treated with inhibitors of respiration and mutants of genes involved in respiration shared similar induction kinetics. Apparently, quantitative comparison of the induction kinetics could be useful to analyze the function of genes as well as to predict the target sites of various chemicals.

    DOI

  • Orthogenomics of Photosynthetic Organisms: Bioinformatic and Experimental Analysis of Chloroplast Proteins of Endosymbiont Origin in Arabidopsis and Their Counterparts in Synechocystis

    Masayuki Ishikawa, Makoto Fujiwara, Kintake Sonoike, Naoki Sato

    PLANT AND CELL PHYSIOLOGY   50 ( 4 ) 773 - 788  2009年04月  [査読有り]

     概要を見る

    Chloroplasts are descendents of a cyanobacterial endosymbiont, but many chloroplast protein genes of endosymbiont origin are encoded by the nucleus. The chloroplastcyanobacteria relationship is a typical target of orthogenomics, an analytical method that focuses on the relationship of orthologous genes. Here, we present results of a pilot study of functional orthogenomics, combining bioinformatic and experimental analyses, to identify nuclear-encoded chloroplast proteins of endosymbiont origin (CPRENDOs). Phylogenetic profiling based on complete clustering of all proteins in 17 organisms, including eight cyanobacteria and two photosynthetic eukaryotes, was used to deduce 65 protein groups that are conserved in all oxygenic autotrophs analyzed but not in non-oxygenic organisms. With the exception of 28 well-characterized protein groups, 56 Arabidopsis proteins and 43 Synechocystis proteins in the 37 conserved homolog groups were analyzed. Green fluorescent protein (GFP) targeting experiments indicated that 54 Arabidopsis proteins were targeted to plastids. Expression of 39 Arabidopsis genes was promoted by light. Among the 40 disruptants of Synechocystis, 22 showed phenotypes related to photosynthesis. Arabidopsis mutants in 21 groups, including those reported previously, showed phenotypes. Characteristics of pulse amplitude modulation fluorescence were markedly different in corresponding mutants of Arabidopsis and Synechocystis in most cases. We conclude that phylogenetic profiling is useful in finding CPRENDOs, but the physiological functions of orthologous genes may be different in chloroplasts and cyanobacteria.

    DOI PubMed CiNii

  • A T-DNA insertion mutant of AtHMA1 gene encoding a Cu transporting ATPase in Arabidopsis thaliana has a defect in the water-water cycle of photosynthesis

    Mieko Higuchi, Hiroshi Ozaki, Minami Matsui, Kintake Sonoike

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   94 ( 3 ) 205 - 213  2009年03月  [査読有り]

     概要を見る

    The water-water cycle is the electron flow through scavenging enzymes for the reactive species of oxygen in chloroplasts, and is proposed to play a role in alternative electron sink in photosynthesis. Here we showed that the water-water cycle is impaired in the T-DNA insertion mutant of AtHMA1 gene encoding a Cu transporting ATPase in chloroplasts. Chlorophyll fluorescence under steady state was not affected in hma1, indicating that photosynthetic electron transport under normal condition was not impaired. Under electron acceptor limited conditions, however, hma1 showed distinguished phenotype in chlorophyll fluorescence characteristics. The most severe phenotype of hma1 could be observed in high (0.1%) CO(2) concentrations, indicating that hma1 has the defect other than photorespiration. The transient increase of chlorophyll fluorescence upon the cessation of the actinic light as well as the NPQ induction of chlorophyll fluorescence revealed that the two pathways of cyclic electron flow around PSI, NDH-pathway and FQR-pathway, are both intact in hma1. Based on the NPQ induction under 0% oxygen condition, we conclude that the water-water cycle is impaired in hma1, presumably due to the decreased level of Cu/Zn SOD in the mutant. Under high CO(2) condition, hma1 exhibited slightly higher NPQ induction than wild type plants, while this increase of NPQ in hma1 was suppressed when hma1 was crossed with crr2 having a defect in NDH-mediated PSI cyclic electron flow. We propose that the water-water cycle and NDH-mediated pathways might be regulated compensationally with each other especially when photorespiration is suppressed. (C) 2008 Elsevier B.V. All rights reserved.

    DOI

  • Mechanism of downregulation of photosystem I content under high-light conditions in the cyanobacterium Synechocystis sp PCC 6803

    Masayuki Muramatsu, Kintake Sonoike, Yukako Hihara

    MICROBIOLOGY-SGM   155 ( 3 ) 989 - 996  2009年03月  [査読有り]

     概要を見る

    Downregulation of photosystem I (PSI) content is an essential process for cyanobacteria to grow under high-light (HL) conditions. In a pmgA (sll 968) mutant of Synechocystis sp. PCC 6803, the levels of PSI content, chlorophyll and transcripts of the psaAB genes encoding reaction-centre subunits of PSI could not be maintained low during HL incubation, although the causal relationship among these phenotypes remains unknown. In this study, we modulated the activity of psaAB transcription or that of chlorophyll synthesis to estimate their contribution to the regulation of PSI content under HL conditions. Analysis of the psaAB-OX strain, in which the psaAB genes were overexpressed under HL conditions, revealed that the amount of psaAB transcript could not affect PSI content by itself. Suppression of chlorophyll synthesis by an inhibitor, laevulinic acid, in the pmgA mutant revealed that chlorophyll availability could be a determinant of PSI content under HL. It was also suggested that chlorophyll content under HL conditions is mainly regulated at the level of 5-aminolaevulinic acid synthesis. We conclude that, upon the shift to HL conditions, activities of psaAB transcription and of 5-aminolaevulinic acid synthesis are strictly downregulated by regulatory mechanism(s) independent of PmgA during the first 6 h, and then a PmgA-mediated regulatory mechanism becomes active after 6 h onward of HL incubation to maintain these activities at a low level.

    DOI PubMed CiNii

  • sll1961 is a novel regulator of phycobilisome degradation during nitrogen starvation in the cyanobacterium Synechocystis sp PCC 6803

    Hanayo Sato, Tamaki Fujimori, Kintake Sonoike

    FEBS LETTERS   582 ( 7 ) 1093 - 1096  2008年04月  [査読有り]

     概要を見る

    The sll1961 gene was reported to encode a regulatory factor of photosystem stoichiometry in the cyanobacterium Synechocystis sp. PCC 6803. We here show that the sll1961 gene is also essential for the phycobilisome degradation during nitrogen starvation. The defect in phycobilisome degradation was observed in the sll1961 mutant despite the increased expression of nhlA, a gene involved in phycobilisome degradation during nitrogen starvation. Photosystem stoichiometry is not affected by nitrogen starvation in the sll1961 mutant nor in the wild-type. The results indicate the presence of a novel pathway for phycobilisome degradation control independent of nblA expression. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI PubMed CiNii

  • The plastid sigma factor SIG1 maintains photosystem I activity via regulated expression of the psaA operon in rice chloroplasts

    Yuzuru Tozawa, Masayoshi Teraishi, Tadamasa Sasaki, Kintake Sonoike, Yoshitaka Nishiyama, Mitsuhiro Itaya, Akio Miyao, Hirohiko Hirochika

    PLANT JOURNAL   52 ( 1 ) 124 - 132  2007年10月  [査読有り]

     概要を見る

    Sigma factors encoded by the nucleus of plants confer promoter specificity on the bacterial-type RNA polymerase in chloroplasts. We previously showed that transcripts of OsSIG1, which encodes one such sigma factor in rice, accumulate relatively late during leaf development. We have now isolated and characterized two allelic mutants of OsSIG1, in which OsSIG1 is disrupted by insertion of the retrotransposon Tos17, in order to characterize the functions of OsSIG1. The OsSIG1(-/-) plants were found to be fertile but they manifested an approximately one-third reduction in the chlorophyll content of mature leaves. Quantitative RT-PCR and northern blot analyses of chloroplast gene expression revealed that the abundance of transcripts derived from the psaA operon was markedly reduced in OsSIG1(-/-) plants compared with that in wild-type homozygotes. This effect was accompanied by a reduction in the abundance of the core protein complex (PsaA-PsaB) of photosystem I. Analysis of chlorophyll fluorescence also revealed a substantial reduction in the rate of electron transfer from photosystem II to photosystem I in the OsSIG1 mutants. Our results thus indicate that OsSIG1 plays an important role in the maintenance of photosynthetic activity in mature chloroplasts of rice by regulating expression of chloroplast genes for components of photosystem I.

    DOI

  • Expression of the algal cytochrome c(6) gene in Arabidopsis enhances photosynthesis and growth

    Hirotaka Chida, Aiko Nakazawa, Hideharu Akazaki, Takako Hirano, Kohei Suruga, Masahiro Ogawa, Tadashi Satoh, Kazunari Kadokura, Seiji Yamada, Wataru Hakamata, Katsunori Isobe, Tei-ichiro Ito, Ryuichi Ishii, Toshiyuki Nishio, Kintake Sonoike, Tadatake Oku

    PLANT AND CELL PHYSIOLOGY   48 ( 7 ) 948 - 957  2007年07月  [査読有り]

     概要を見る

    Photosynthetic plants convert light energy into ATP and NADPH in photosynthetic electron transfer and photophosphorylation, and synthesize mainly carbohydrates in the Calvin - Benson cycle. Here we report the enhancement of photosynthesis and growth of plants by introducing the gene of an algal cytochrome c(6), which has been evolutionarily eliminated from higher plant chloroplasts, into the model plant Arabidopsis thaliana. At 60 d after planting, the plant height, leaf length and root length of the transformants were 1.3-, 1.1- and 1.3- fold those in the wild- type plants, respectively. At the same time, in the transgenic plants, the amounts of chlorophyll, protein, ATP, NADPH and starch were 1.2-, 1.1-, 1.9-, 1.4- and 1.2- fold those in the wild- type plants, respectively. The CO2 assimilation capacity of the transgenic plants was 1.3- fold that of the wild type. Moreover, in transgenic Arabidopsis expressing algal cytochrome c(6), the 1 - qP, which reflects the reduced state of the plastoquinone pool, is 30% decreased compared with the wild type. These results show that the electron transfer of photosynthesis of Arabidopsis would be accelerated by the expression of algal cytochrome c(6). Our results demonstrate that the growth and photosynthesis of Arabidopsis plants could be enhanced by the expression of the algal cytochrome c(6) gene.

    DOI PubMed CiNii

  • Pacific Ocean and Japan Sea ecotypes of Japanese beech (Fagus crenata) differ in photosystem responses to continuous high light

    Jun-Ya Yamazaki, Etsuko Yoda, Ayako Takahashi, Kintake Sonoike, Emiko Maruta

    TREE PHYSIOLOGY   27 ( 7 ) 961 - 968  2007年07月  [査読有り]

     概要を見る

    Two ecotypes of Japanese beech (Fagus crenata Blume), the Pacific Ocean type (PAO) and the Japan Sea type (JAS), show different responses to high solar irradiance. When PAO and JAS saplings were grown in continuous high-light (H), leaves of JAS became pale green. To elucidate this phenomenon, we investigated in vivo photochemistry based on pigment concentrations of Photosystem (PS) I and PS II and Western blot analysis. In JAS-H leaves, the amount of D1-protein decreased, resulting in decreases in the maximal quantum yield of PS II (F-v/F-m) and electron transport rate, whereas PAO-H leaves maintained high activities. The PS I photochemistry determined by measurement of P-700 photo-oxidation showed that the intersystem electron pool size was 1.4 times greater in JAS-H leaves than in PAO-H leaves. Furthermore, the re-reduction kinetics of P-700(+) showed that cyclic electron transport around PS I was 1.2 times faster in PAO-H leaves than in JAS-H leaves. Analysis of the area over the fluorescence induction kinetics indicated that the relative abundance of the PS II alpha center increased in PAO-H leaves, whereas JAS leaves were observed to have low acclimation capacity to high light. These results demonstrate that PAO leaves possess acclimation mechanisms to continuous high light, whereas JAS leaves are more vulnerable to continuous high light, resulting in reduced leaf longevity owing to photoinhibition caused by increases in the intersystem electron pool size and suppression of photochemistry at the level of PS I and PS II.

    DOI

  • Large-scale analysis of chlorophyll fluorescence kinetics in Synechocystis sp PCC 6803: Identification of the factors involved in the modulation of photosystem stoichiometry

    Hiroshi Ozaki, Masahiko Ikeuchi, Teruo Ogawa, Hideya Fukuzawa, Kintake Sonoike

    PLANT AND CELL PHYSIOLOGY   48 ( 3 ) 451 - 458  2007年03月  [査読有り]

     概要を見る

    Since chlorophyll fluorescence reflects the redox state of photosynthetic electron transport chain, monitoring of chlorophyll fluorescence has been successfully applied for the screening of photosynthesis-related genes. Here we report that the mutants having a defect in the regulation of photosystem stoichiometry could be identified through the simple comparison of the induction kinetics of chlorophyll fluorescence. We made a library containing 500 mutants in the cyanobacterium Synechocystis sp. PCC 6803 with transposon-mediated gene disruption, and the mutants were used for the measurement of chlorophyll fluorescence kinetics for 45 s. We picked up two genes, pmgA and sll1961, which are involved in the modulation of photosystem stoichiometry. The disruptants of the two genes share common characteristics in their fluorescence kinetics, and we searched for mutants that showed such characteristics. Out of six mutants identified so far, five showed a different photosystem stoichiometry under high-light conditions. Thus, categorization based on the similarity of fluorescence kinetics is an excellent way to identify the function of genes.

    DOI PubMed CiNii

  • Functional roles of plastid SIG1 for gene expression of photosystem I components

    Yuzuru Tozawa, Masayoshi Teraishi, Tadamasa Sasaki, Kintake Sonoike

    PLANT AND CELL PHYSIOLOGY   48   S35 - S35  2007年  [査読有り]

  • Chloroplast NAD kinase is essential for energy transduction through the xanthophyll cycle in photosynthesis

    Hideyuki Takahashi, Ayako Watanabe, Ayumi Tanaka, Shin-nosuke Hashida, Maki Kawai-Yamada, Kintake Sonoike, Hirofumi Uchimiya

    PLANT AND CELL PHYSIOLOGY   47 ( 12 ) 1678 - 1682  2006年12月  [査読有り]

     概要を見る

    Photosynthetic parameters of the nadk2 mutant of Arabidopsis thaliana, which is defective in chloroplast NAD kinase, were investigated. In this plant, the effective efficiency of photosynthetic electron transport (Phi II) and the quantum yield of open reaction centers of photosystem II (Fv'/Fm') were decreased. Furthermore, an increase in nonphotochemical quenching attributed to energy dissipation from the xanthophyll cycle was observed. The mutant showed an aberrant de-epoxidation state of xanthophyll cycle carotenoids and had a high level of zeaxanthin even under low light conditions. These results indicate that chloroplast NAD kinase, catalyzing phosphorylation of NAD, is essential for the proper photosynthetic machinery of PSII and the xanthophyll cycle.

    DOI PubMed CiNii

  • High-dimensional and large-scale phenotyping of yeast mutants

    Y Ohya, J Sese, M Yukawa, F Sano, Y Nakatani, TL Saito, A Saka, T Fukuda, S Ishihara, S Oka, G Suzuki, M Watanabe, A Hirata, M Ohtani, H Sawai, N Fraysse, JP Latge, JM Francois, M Aebi, S Tanaka, S Muramatsu, H Araki, K Sonoike, S Nogami, S Morishita

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   102 ( 52 ) 19015 - 19020  2005年12月  [査読有り]

     概要を見る

    One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

    DOI PubMed CiNii

  • The mutant of sll1961, which encodes a putative transcriptional regulator, has a defect in regulation of photosystem stoichiometry in the cyanobacterium Synechocystis sp PCC 6803

    T Fujimori, M Higuchi, H Sato, H Aiba, M Muramatsu, Y Hihara, K Sonoike

    PLANT PHYSIOLOGY   139 ( 1 ) 408 - 416  2005年09月  [査読有り]

     概要を見る

    In acclimation to changing light environments, photosynthetic organisms modulate the ratio of two photosynthetic reaction centers ( photosystem IIPSI] and photosystem II). One mutant, which could not modulate photosystem stoichiometry upon the shift to high light, was isolated from mutants created by random transposon mutagenesis. Measurements of chlorophyll fluorescence and analysis of the reaction center subunits of PSI through western blotting in this mutant revealed that the content of PSI could not be suppressed under high-light condition. In the mutant, transposon was inserted to the sll1961 gene encoding a putative transcriptional regulator. DNA microarray analysis revealed that the expression of sll1773 was drastically induced in the sll1961 mutant upon exposure to high light for 3 h. Our results demonstrate that a transcriptional regulator, Sll1961, and its possible target proteins, including Sll1773, may be responsible for the regulation of photosystem stoichiometry in response to high light.

    DOI PubMed CiNii

  • PsaK2 subunit in photosystem I is involved in state transition under high light condition in the cyanobacterium Synechocystis sp PCC 6803

    T Fujimori, Y Hihara, K Sonoike

    JOURNAL OF BIOLOGICAL CHEMISTRY   280 ( 23 ) 22191 - 22197  2005年06月  [査読有り]

     概要を見る

    To avoid the photodamage, cyanobacteria regulate the distribution of light energy absorbed by phycobilisome antenna either to photosystem II or to photosystem I ( PSI) upon high light acclimation by the process so-called state transition. We found that an alternative PSI subunit, PsaK2 (sll0629 gene product), is involved in this process in the cyanobacterium Synechocystis sp. PCC 6803. An examination of the subunit composition of the purified PSI reaction center complexes revealed that PsaK2 subunit was absent in the PSI complexes under low light condition, but was incorporated into the complexes during acclimation to high light. The growth of the psaK2 mutant on solid medium was inhibited under high light condition. We determined the photosynthetic characteristics of the wild type strain and the two mutants, the psaK1 ( ssr0390) mutant and the psaK2 mutant, using pulse amplitude modulation fluorometer. Non-photochemical quenching, which reflects the energy transfer from phycobilisome to PSI in cyanobacteria, was higher in high light grown cells than in low light grown cells, both in the wild type and the psaK1 mutant. However, this change of non-photochemical quenching during acclimation to high light was not observed in the psaK2 mutant. Thus, PsaK2 subunit is involved in the energy transfer from phycobilisome to PSI under high light condition. The role of PsaK2 in state transition under high light condition was also confirmed by chlorophyll fluorescence emission spectra determined at 77 K. The results suggest that PsaK2-dependent state transition is essential for the growth of this cyanobacterium under high light condition.

    DOI PubMed CiNii

  • Mass identification of chloroplast proteins of endosymbiont origin by phylogenetic profiling based on organism-optimized homologous protein groups.

    Sato N, Ishikawa M, Fujiwara M, Sonoike K

    Genome informatics. International Conference on Genome Informatics   16 ( 2 ) 56 - 68  2005年  [査読有り]  [国内誌]

    DOI PubMed CiNii J-GLOBAL

  • A cyanobacterial gene encoding an ortholog of Pirin is induced under stress conditions

    Y Hihara, M Muramatsu, K Nakamura, K Sonoike

    FEBS LETTERS   574 ( 1-3 ) 101 - 105  2004年09月  [査読有り]

     概要を見る

    Pirin is a recently identified protein in eukaryotes as a transcription cofactor or as an apoptosis-related protein. Although Pirin is highly conserved from bacteria to human, there have been no reports on prokaryotic Pirin orthologs. We show here that pirA (sll1773) encoding an ortholog of Pirin together with an adjacent gene, pirB (ssl3389), was upregulated under high salinity and some other stress conditions in a cyanobacterium Synechocystis sp. PCC 6803. Induction of the pirAB genes was not related to cell death and disruption of pirA did not affect the gene expression profile. Expression of the pirAB genes was negatively regulated by a LysR family transcriptional regulator encoded by pirR (slr1871) located immediately upstream of pirAB in the divergent direction. DNA microarray analysis indicated that PirR repressed expression of closely located ORFs, slr1870 and mutS (sll1772), in addition to pirAB and pirR itself. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

    DOI

  • Decrease in the efficiency of the electron donation to tyrosine Z of photosystem II in an SQDG-deficient mutant of Chlamydomonas

    A Minoda, K Sonoike, K Okada, N Sato, M Tsuzuki

    FEBS LETTERS   553 ( 1-2 ) 109 - 112  2003年10月  [査読有り]

     概要を見る

    Photosystem (PS) II activity of a sulfoquinovosyl diacylgiycerol (SQDG)-deficient mutant (hf-2) of Chlamydomonas was partially decreased compared with that of wild-type. The susceptibility to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was also modified in the mutant. Photometric measurements in the isolated thylakoid membranes of hf-2 revealed that the lowered activity in the mutant was derived from a decrease in the efficiency of the electron donation from water to tyrosine Z, not from the efficiency of the electron transport from Q(A) to Q(B). This result was confirmed by the decay kinetics of chlorophyll fluorescence determined in vivo. We conclude that SQDG contributes to maintaining the conformation of PSII complexes, particularly that of D1 polypeptides, which are necessary for maximum activities in Chlamydomonas. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

    DOI PubMed CiNii

  • Over-reduced states of the Mn-cluster in cucumber leaves induced by dark-chilling treatment

    Mieko Higuchi, Takumi Noguchi, Kintake Sonoike

    Biochimica et Biophysica Acta - Bioenergetics   1604 ( 3 ) 151 - 158  2003年07月  [査読有り]

     概要を見る

    Oxygen evolution is inhibited when leaves of chilling-sensitive plants like cucumber are treated at 0°C in the dark. The activity is restored by moderate illumination at room temperature. We examined the changes in the redox state of the Mn-cluster in cucumber leaves in the processes of dark-chilling inhibition and subsequent light-induced reactivation by means of thermoluminescence (TL). A TL B-band arising from S2QB - charge recombination in PSII was observed upon single-flash illumination of untreated leaves, whereas four flashes were required to yield the B-band after dark-chilling treatment for 24 h. This three-step delay indicates that over-reduced states of the Mn-cluster such as the S-2 state were formed during the treatment. Fitting analysis of the flash-number dependence of the TL intensities showed that the Mn-cluster was more reduced with a longer period of the treatment and that S-3 was the lowest S-state detectable in the dark-chilled leaves. Measurements of the Mn content by atomic absorption spectroscopy showed that Mn atoms were gradually released from PSII during the dark-chilling treatment but re-bound to PSII by illumination at 30°C. Thus, dark-chilling inhibition of oxygen evolution can be ascribed to the disintegration of the Mn-cluster due to its over-reduction. The observation of the S-3 state in the present in vivo system strongly suggests that S-3, which has been observed only by addition of exogenous reductants into in vitro preparations, is indeed a redox intermediate of the Mn-cluster in the processes of its disintegration and photoactivation. © 2003 Elsevier Science B.V. All rights reserved.

    DOI PubMed CiNii

  • Over-reduced states of the Mn-cluster in cucumber leaves induced by dark-chilling treatment.

    Higuchi M, Noguchi T, Sonoike K

    Biochimica et biophysica acta   1604 ( 3 ) 151 - 158  2003年07月  [査読有り]

    DOI PubMed

  • Involvement of sulfoquinovosyl diacylglycerol in the structural integrity and heat-tolerance of photosystem II

    N Sato, M Aoki, Y Maru, K Sonoike, A Minoda, M Tsuzuki

    PLANTA   217 ( 2 ) 245 - 251  2003年06月  [査読有り]

     概要を見る

    To examine the role of sulfoquinovosyl diacylglycerol (SQDG) in thylakoid membranes, we compared the structural and functional properties of photosystem II (PSII) between a mutant of Chlamydomonas reinhardtii defective in SQDG (hf-2) and the wild type. The PSII core complex of hf-2, as compared with that of the wild type, showed structural fragility when solubilized with a detergent, dodecyl beta-D-altoside, suggesting that the physical properties of the PSII complex were altered by the loss of SQDG. On the other hand, exposure of the cells to 41 degreesC for 120 min in the dark decreased the PSII activity to 70% and 50% of the initial levels in the wild type and hf-2, respectively, which implies that the PSII activity, in the absence of SQDG, becomes less stable under heat-stress conditions. PSII inactivated to 60% of the initial level by dark incubation at 41 degreesC was reactivated by following illumination even at 41 degreesC to more than 90% in the wild type, but only to 70% in hf-2. These results suggest that PSII inactivated by heat recovers through some mechanism dependent on light, and that SQDG participates in functioning of the mechanism. The conformational disorder of PSII caused by the defect in SQDG might be correlated with the increased susceptibility of its activity to heat-stress.

    DOI

  • DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp strain PCC 6803

    Y Hihara, K Sonoike, M Kanehisa, M Ikeuchi

    JOURNAL OF BACTERIOLOGY   185 ( 5 ) 1719 - 1725  2003年03月  [査読有り]

     概要を見る

    Whole-genome DNA microarrays were used to evaluate the effect of the redox state of the photosynthetic electron transport chain on gene expression in Synechocystis sp. strain PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures, and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change in the redox state of the photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. strain PCC 6803.

    DOI PubMed CiNii

  • Binding and functional properties of the extrinsic proteins in oxygen-evolving photosystem II particle from a green alga, Chlamydomonas reinhardtii having His-tagged CP47

    T Suzuki, J Minagawa, T Tomo, K Sonoike, H Ohta, Enami, I

    PLANT AND CELL PHYSIOLOGY   44 ( 1 ) 76 - 84  2003年01月  [査読有り]

     概要を見る

    Oxygen-evolving photosystem 11 (PSII) particles were purified from Chlamydomonas reinhardtii having His-tag extension at the C terminus of the CP47 protein, by a single-step Ni2(+)-affinity column chromatography after solubilization of thylakoid membranes with sucrose monolaurate. The PSII particles consisted of, in addition to intrinsic proteins, three extrinsic proteins of 33, 23 and 17 kDa. The preparation showed a high oxygen-evolving activity of 2,300-2,500 mumol O-2 (mg Chl)(-1) h(-1) in the presence of Ca2+ using ferricyanide as the electron acceptor, while its activity was 680-720 mumol O-2 (mg Chl)(-1) h(-1) in the absence of Ca2+ and Cl- ions. The activity was 710-820 mumol O-2 (mg Chl)(-1) h(-1) independent of the presence or absence of Ca2+ and Cl when 2,6-dichloro-p-benzoquinone was used as the acceptor. These activities were scarcely inhibited by DCMU. The kinetics of flash-induced fluorescence decay revealed that the electron transfer from Q(A)(-) to Q(B), was significantly inhibited, and the electron transfer from Q(A)(-) to ferricyanide was largely stimulated in the presence of Ca2+. These results indicate that the acceptor side, Q(B) site, was altered in the PSII particles but its donor side remained intact. Release-reconstitution experiments revealed that the extrinsic 23 and 17 kDa proteins were released only partially by NaCl-wash, while most of the three extrinsic proteins were removed when treated with urea/NaCl, alkaline Tris or CaCl2- The 23 and 17 kDa proteins directly bound to PSII independent of the other extrinsic proteins, and the 33 kDa protein functionally re-bound to CaCl2-treated PSII which had been reconstituted with the 23 and 17 kDa proteins. These binding properties were largely different from those of the extrinsic proteins in higher plant PSII, and suggest that each of the three extrinsic proteins has their own binding sites independent of the others in the green algal PSII.

    DOI PubMed CiNii

  • Irreversible damage to photosystem I by chilling in the light: cause of the degradation of chlorophyll after returning to normal growth temperature

    H Kudoh, K Sonoike

    PLANTA   215 ( 4 ) 541 - 548  2002年08月  [査読有り]

     概要を見る

    The recovery process after chilling-induced photoinhibition of photosystem I (PSI) was studied in leaves of a chilling-sensitive plant, cucumber (Cucumis sativits L. cv. Nanshin). Determination of chlorophyll content, photosystem (PS) activities in vivo and in vitro, and the amount OF reaction-center subunits of PSI revealed that: (i) The content of chlorophyll decreased to 70% of the original level gradually from 1 to 3 days after exposure to a low temperature. (ii) The amount of functional PSI per unit leaf area was reduced to 30% of the initial level by the chilling treatment. The amount of functional PSI gradually increased during the next 6 days but only to 50% of the original level. (iii) When expressed on a chlorophyll basis, however, the amount of functional PSI recovered to 90% of the original level 6 days after the treatment. (iv) The residual amount of chlorophyll on the third day after the treatment closely correlated with the amount of functional PSI at that point. These results indicate that the decrease in chlorophyll content at a normal growth temperature after chilling treatment is a consequence of the degradation of irreversibly damaged PSI complexes. Immunoblot analysis confirmed that PsaAB protein, the reaction-center subunits of PSI, was degraded during the 3 days after chilling treatment. Some characteristics of the chilling injury frequently reported, i.e. irreversibility of the injury and development of visible symptoms at room temperature, can be explained as a consequence of the chilling-induced photoinhibition of PSI.

    DOI

  • Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii

    A Minoda, N Sato, H Nozaki, K Okada, H Takahashi, K Sonoike, M Tsuzuki

    EUROPEAN JOURNAL OF BIOCHEMISTRY   269 ( 9 ) 2353 - 2358  2002年05月  [査読有り]

     概要を見る

    The physiological role of sulfoquinovosyl diacylglycerol (SQDG) in photosynthesis was investigated with a SQDG defective mutant (hf -2) of Chlamydomonas reinhardtii that did not have any detectable amount of SQDG. The mutant showed a lower rate of photosystem II (PSII) activity by approximate to 40% and also a lower growth rate than those of the wild-type. Results of genetical analysis of hf -2 strongly suggest that the SQDG defect and the lowered PSII activity are due to a single gene mutation. The supplementation of SQDG to hf -2 cells restored the lowered PSII activity to the same level as that of wild-type cells, and also enabled the mutant to grow even in the presence of 135 nm 3-(3,4-dichlorophenyl)-1,1-dimethylurea. Moreover, the incubation of isolated thylakoid membranes of hf -2 with SQDG raised the lowered PSII activity. Chemical modifications of SQDG impaired the recovery of PSII activity. The results suggest that SQDG is indispensable for PSII activity in Chlamydomonas by maintaining PSII complexes in their proper state.

    DOI PubMed CiNii

  • Erratum: Role of sulfoquinovosyl diacylglycerol for the maintenance of photosystem II in Chlamydomonas reinhardtii (European Journal of Biochemistry (2002) 269: 9 (2353-2358))

    Minoda A, Sato N, Nozaki H, Okada K, Takahashi H, Sonoike K, Tsuzuki M

    European Journal of Biochemistry   269 ( 12 )  2002年  [査読有り]

    DOI

  • Dark-chilling pretreatment protects PSI from light-chilling damage

    Kudoh, H, Sonoike, K

    Journal of Photoscience   9   59 - 62  2002年

  • Physiological significance of the regulation of photosystem stoichiometry upon high light acclimation of Synechocystis sp PCC 6803

    K Sonoike, Y Hihara, M Ikeuchi

    PLANT AND CELL PHYSIOLOGY   42 ( 4 ) 379 - 384  2001年04月  [査読有り]

     概要を見る

    We characterized the photosynthetic properties of the pmgA mutant of Synechocystis PCC 6803, which cannot change its photosystem stoichiometry under a high-light condition (200 mu mol m(-2) s(-1)), in order to clarify the physiological significance of the regulation of photosystem stoichiometry. We found that (1) PSII activity was inhibited more in wild-type cells on the first day under the high-light conditions than in mutant cells. (2) The growth of the mutants following the initial imposition of high light was faster than that of wild-type cells. (3) However, growth was severely inhibited in the mutants after the third day of exposure to high light, (4) The growth inhibition in the mutants under the extended high-light conditions was reversed by the addition of sublethal concentrations of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), which seemed to mimic photoinhibition of PSII, These results suggest that the main role of adjusting the photosystem stoichiometry with respect to light intensity is not to maintain efficient photosynthesis, but to down regulate electron transfer. Failure to down regulate electron flow leads to cell death under prolonged exposure to high light in this cyanobacterium.

    DOI PubMed CiNii

  • Role of pyrenoids in the CO2-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (volvocales)

    E Morita, T Abe, M Tsuzuki, S Fujiwara, N Sato, A Hirata, K Sonoike, H Nozaki

    PLANTA   208 ( 3 ) 365 - 372  1999年05月  [査読有り]

     概要を見る

    The morphology of the pyrenoid and the physiology of the CO2-concentrating mechanism (CCM) were investigated in Chlamydomonas (Cd.) mutabilis Gerloff UTEX 578, Cd. radiata Deason et Bold UTEX 966, Cd. augustae Skuja UTEX 1969, Cd. macrostellata Lund SAG 72.81, Cd, bipapillata Bourrelly SAG 11-47, and Chloromonas (Cr.) insignis Gerloff et Ettl NIES-447, all of which are closely related phylogenetically to the pyrenoid-less strains of Chloromonas. In the chloroplasts of Cd. mutabilis UTEX 578, Cd. radiata UTEX 966, Cd. augustae UTEX 1969, and Cd. macrostellata SAG 72.81, a typical, spheroidal, electron-dense pyrenoid matrix surrounded by starch granules was present, and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) molecules were highly concentrated in the pyrenoid matrix. On the other hand, while the pyrenoid matrix of CI, insignis NIES-447 was electron-dense that of Cd. bipapillata SAG 11-47 was not, and neither was surrounded by starch granules. The pyrenoid matrices of these two species exhibited a higher concentration of Rubisco molecules than the thylakoid region (thylakoid and stroma) of the chloroplasts; however, the densities of Rubisco molecules in these pyrenoid matrices were low compared with those of the other four Chlamydomonas strains examined in this study and that of Cd. reinhardtii Dangeard. In all six strains examined, the presence of the CCM was indicated by relatively high photosynthetic affinities for CO2 (low values of K-0.5(CO2)). However, differences in the inorganic carbon (Ci) pools were recognized in relation to the differences in pyrenoid morphology among the strains. In the typical pyrenoid-containing strains. Cd. mutabilis UTEX 578 and Cn. radiata UTEX 966, the ratio of internal to external inorganic carbon was about 20, while in Cr. insignis NIES-447 and Cd. bipapillata SAG 11-47 the ratio was only 2-3 similar to the two pyrenoid-less, CCM-containing strains of Chloromonas previously examined (E. Morita et al., 1998, Planta 204. 269-276). It is thus speculated that the presence of typical pyrenoids with a high concentration of Rubisco molecules is related to the formation of large Ci pools in the CCM. Detailed phylogenetic relationships among these Chlamydomonas/Chloromonas strains and the pyrenoid-less Chloromonas strains previously investigated were inferred based on the sequence of rbcL, the gene for the large subunit of Rubisco. Two monophyletic groups were resolved with high bootstrap values. Based on the tree topology resolved, it was inferred that loss of the typical pyrenoids accompanied by a decrease in intracellular Ci pools might have taken place independently in the two groups.

    DOI CiNii

  • The different roles of chilling temperatures in the photoinhibition of photosystem I and photosystem II

    K Sonoike

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   48 ( 2-3 ) 136 - 141  1999年02月  [査読有り]

     概要を見る

    The role of chilling temperatures on photoinhibition of photosystems I and II (PSI and PSII) under weak light has been examined in cucumber, a chilling-sensitive plant. The extent of PSII photoinhibition, determined by pulse-modulated fluorescence in vivo, is closely related to the redox state of the PSII electron acceptor Q(A), measured as a fluorescence parameter, 1-q(P). On the other hand, the extent of PSI photoinhibition, which is only observed in chilling-sensitive plants at chilling temperatures, cannot be related to the redox state of Q(A), suggesting that the underlying mechanism is different from that of PSII photoinhibition. Chilling treatment at low photon flux densities is found to enhance cyclic electron flow around PSI. Both PSI photoinhibition and enhanced cyclic electron flow show similar temperature dependence, with the threshold temperature at 10 degrees C. (C) 1999 Elsevier Science S.A. All rights reserved.

    DOI CiNii

  • A novel gene, pmgA, specifically regulates photosystem stoichiometry in the cyanobacterium Synechocystis species PCC 6803 in response to high light

    Y Hihara, K Sonoike, M Ikeuchi

    PLANT PHYSIOLOGY   117 ( 4 ) 1205 - 1216  1998年08月  [査読有り]

     概要を見る

    Previously, we identified a novel gene, pmgA, as an essential factor to support photomixotrophic growth of Synechocystis species PCC 6803 and reported that a strain in which pmgA was deleted grew better than the wild type under photoautotrophic conditions. To gain insight into the role of pmgA, we investigated the mutant phenotype of pmgA in detail. When low-light-grown (20 mu E m(-2) s(-1)) cells were transferred to high light (HL [200 mu E m(-2) s(-1)]), pmgA mutants failed to respond in the manner typically associated with Synechocystis. Specifically, mutants lost their ability to suppress accumulation of chlorophyll and photosystem I and, consequently, could not modulate photosystem stoichiometry. These phenotypes seem to result in enhanced rates of photosynthesis and growth during short-term exposure to HL. Moreover, mixed-culture experiments clearly demonstrated that loss of pmgA function was selected against during longer-term exposure to HL, suggesting that pmgA is involved in acquisition of resistance to HL stress. Finally, early induction of pmgA expression detected by reverse transcriptase-PCR upon the shift to HL led us to conclude that pmgA is the first gene identified, to our knowledge, as a specific regulatory factor for HL acclimation.

    DOI PubMed CiNii

  • Presence of the CO2-concentrating mechanism in some species of the pyrenoid-less free-living algal genus Chloromonas (Volvocales, Chlorophyta)

    E Morita, T Abe, M Tsuzuki, S Fujiwara, N Sato, A Hirata, K Sonoike, H Nozaki

    PLANTA   204 ( 3 ) 269 - 276  1998年03月  [査読有り]

     概要を見る

    Physiological and morphological characteristics related to the CO2-concentrating mechanism (CCM) were examined in several species of the free-living, unicellular volvocalean genus Chloromonas (Chlorophyta), which differs morphologically from the genus Chlamydomonas only by lacking pyrenoids. The absence of pyrenoids in the chloroplasts of Chloromonas (Cr.) rosae UTEX 1337, Cr. serbinowii UTEX 492, Cr. clatharata UTEX 1970, Cr. rosae SAG 26.90, and Cr. palmelloides SAG 32.86 was confirmed by light and electron microscopy. In addition, immunogold electron microscopy demonstrated that ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1.39) molecules were distributed almost evenly throughout the chloroplasts in all five Chloromonas strains. However, Chloromonas exhibited two types of physiological characteristics related to the CCM depending on the species or strains examined. Chloromonas rosae UTEX 1337 and Cr. serbinowii had high photosynthetic affinities for CO2 in cells grown in culture medium bubbled with air (low-CO2 cells), compared with those grown in medium bubbled with 5% CO2 (high-CO2 cells), indicating the presence of the low-CO2-inducible CCM. In addition, these two Chloromonas strains exhibited low-CO2-inducible carbonic anhydrase (CA; EC 4.2.1.1) activity and seemed to have small intracellular inorganic carbon pools. Therefore, it appears that Cr. rosae UTEX 1337 and Cr. serbinowii possess the CCM as in pyrenoid-containing microalgae such as Chlamydomonas reinhardtii. By contrast, Cr. clatharata, Cr. rosae SAG 26.90 and Cr. palmelloides showed low photosynthetic affinities for CO2 when grown under both CO2 conditions. Moreover, these three strains exhibited an apparent absence of intracellular inorganic carbon pools and lacked low-CO2-inducible CA activity. Thus, Cr. clatharata, Cr. rosae SAG 26.90 and Cr. palmelloides, like other pyrenoid-less algae (lichen photobionts) reported previously, seem to lack the CCM. The present study is the first demonstration of the CCM in pyrenoid-less algae, indicating that pyrenoids or accumulation of Rubisco in the chloroplasts are not always essential for the CCM in algae. Focusing on this type of CCM in pyrenoid-less algae, the physiological and evolutionary significance of pyrenoid absence is discussed.

    DOI PubMed CiNii

  • Various aspects of inhibition of photosynthesis under light/chilling stress: "Photoinhibition at chilling temperatures" versus "Chilling damage in the light"

    K Sonoike

    JOURNAL OF PLANT RESEARCH   111 ( 1101 ) 121 - 129  1998年03月  [査読有り]  [招待有り]

     概要を見る

    At chilling temperatures, plants suffer damage to photosynthesis. The sites and the mechanisms involved in this damage differ under different chilling conditions. The current status of our understanding of this damage is reviewed, and how chilling temperatures affect photosynthesis is discussed with emphasis on the role of light and the phase separation of membrane lipids.

    DOI

  • Effects of virus infection on photosynthesis of Eupatorium makinoi.

    S Funayama-Noguchi, K Sonoike, Terashima, I

    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V     2757 - 2760  1998年  [査読有り]

  • Photosynthetic properties of leaves of Eupatorium makinoi infected by a geminivirus

    S Funayama, K Sonoike, Terashima, I

    PHOTOSYNTHESIS RESEARCH   53 ( 2-3 ) 253 - 261  1997年09月  [査読有り]

     概要を見る

    We examined photosynthetic properties of Eupatorium makinoi leaves infected by a geminivirus. Since a major symptom of the geminivirus infection is variegation or yellowing of leaves, Chi content was used as an index of disease severity. As leaf Chi content was lowered, leaf absorptance, maximal quantum yield of photosynthesis on an absorbed quantum basis (phi O-2,(max)) and light-saturated rate of photosynthesis (P-max) decreased. The share of energy allocated to PS II, which can be estimated from fluorescence parameters and oxygen evolution rate, was about 30% lower in the infected yellow leaves than in uninfected leaves. Analyses of the composition of thylakoid polypeptides by gel electrophoresis showed preferential loss of LHC II. The lower phi O-2,(max) in the infected leaves was, thus, attributed to the decreased energy allocation to PS II. These features were largely consistent with those of b-less mutants, but lowered P-max has been never reported for b-less mutants. Possible mechanisms causing these changes in photosynthetic properties to the infected leaves are discussed.

    DOI CiNii

  • The mechanism of the degradation of psaB gene product, one of the photosynthetic reaction center subunits of Photosystem I, upon photoinhibition

    K Sonoike, M Kamo, Y Hihara, T Hiyama, Enami, I

    PHOTOSYNTHESIS RESEARCH   53 ( 1 ) 55 - 63  1997年07月  [査読有り]

     概要を見る

    The psaB gene product (PsaB protein), one of the reaction center subunits of Photosystem I (PS I), was specifically degraded by light illumination of spinach thylakoid membranes. The degradation of the protein yielded N-terminal fragments of molecular mass 51 kDa and 45 kDa. The formation of the 51 kDa fragment was i) partially suppressed by the addition of phenylmethylsulfonyl fluoride or 3,4-dichloroisocoumarin, which are inhibitors of serine proteases, and ii) enhanced in the presence of hydrogen peroxide during photoinhibitory treatment, but iii) not detected following hydrogen peroxide treatment in the dark. These results suggest that the hydroxyl radical produced at the reduced iron-sulfur centers in PS I triggers the conformational change of the PS I complex, which allows access of a serine-type protease to PsaB. This results in the formation of the 51 kDa N-terminal fragment, presumably by cleavage on the loop exposed to the stromal side, between putative helices 8 and 9. On the other hand, the formation of the 45 kDa fragment, which was enhanced in the presence of methyl viologen but did not accompany the photoinhibition of PS I, was not affected by the addition of hydrogen peroxide or protease inhibitors. Another fragment of 18 kDa was identified as a C-terminal counterpart of the 45 kDa fragment. N-terminal sequence analysis of the 18 kDa fragment revealed that the cleavage occurred between Ala(500) and Val(501) on the loop exposed to the lumenal side, between putative helices 7 and 8 of the PsaB protein.

    DOI

  • The inhibition of photosynthesis after exposure of bean leaves to various low levels of CO2

    M Ishibashi, K Sonoike, A Watanabe

    PLANT AND CELL PHYSIOLOGY   38 ( 5 ) 619 - 624  1997年05月  [査読有り]

     概要を見る

    Mature first leaves of Phaseolus vulgaris L. were exposed to low partial pressures of CO2 (7, 6 and 0 Pa CO2) for 24 h. After exposure of leaves to 6 Pa CO2 for 24 h, there was a reduction in the carbon exchange rate (CER) at all partial pressures of CO2 at which measurements were made, After exposure to 7 Pa CO2, the CER decreased only at high partial pressures of CO2. The rates of electron transport from water to methyl viologen, through the whole chain, decreased in parallel with the decrease in CER measured at 90 Pa CO2. One site of inhibition in leaves exposed for 24 h to 6 Pa CO2 appeared to be the intersystem electron-transport chain since there were no significant changes in the activities of PSI and PSII, as determined from the level of P-700 and measurement of fluorescence, respectively, Another inhibitory phenomenon appeared to be a negative change in the activation state of Rubisco, while the level of Rubisco was unaffected by the exposure to 6 Pa CO2. These decreases in photosynthetic activity caused by depletion of CO2 explains at least in part, the inhibition of photosynthesis that is caused by rain treatment [Ishibashi and Terashima (1995) Plant Cell Environ. 18: 431].

    DOI

  • Photoinhibition of photosynthesis during rain treatment: Identification of the intersystem electron-transfer chain as the site of inhibition

    Momoe Ishibashi, Kintake Sonoike, Akira Watanabe

    Plant and Cell Physiology   38 ( 2 ) 168 - 172  1997年  [査読有り]

     概要を見る

    Continuous wetness of leaves in the light causes a reduction in the carbon exchange rate (CER) in Phaseolus vulgaris L. [Ishibashi and Terashima (1995) Plant Cell Environ. 18: 431]. In this study, we investigated the initial cause of photoinhibition upon application of water, designated rain treatment, and we found a large decrease in the rate of electron transport through the whole chain from water to methyl viologen via PSII and PSI. In spite of the decrease in the rate of electron transport, there was no decrease in the activity of either PSI or PSII when these activities were measured separately. The intactness of PSI was also confirmed by the absence of any change in the photooxidizable amount of P-700, the reaction centre of PSI, and the intactness of PSII was confirmed by measurements of Chl fluorescence. The results suggest that the inhibition by the rain treatment, which occurs at the site between PSI and PSII, might be a novel type of photoinhibition, unlike the conventional types of photoinhibition that involve PSI and PSII.

    DOI

  • Contribution of lowered unsaturation levels of chloroplast lipids to high temperature tolerance of photosynthesis in Chlamydomonas reinhardtii

    N Sato, K Sonoike, A Kawaguchi, M Tsuzuki

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   36 ( 3 ) 333 - 337  1996年12月  [査読有り]

     概要を見る

    A mutant of Chlamydomonas reinhardtii designated as hf-9 is impaired in fatty acid desaturation of chloroplasts, and showed lowered unsaturation levels of chloroplast lipids, as compared with the parent (Sate et al., Eur. J. Biochem., 230 (1995) 987-993). The effects of temperature on photosynthesis were compared between hf-9 and the parent for investigation whether or not unsaturation levels of chloroplast lipids are correlated with the thermal properties of photosynthesis. Growth rates determined by turbidity were higher in the parent than in hf-9 at both 10 and 24 degrees C, while similar for the parent and hf-9 at 39 degrees C. The cells grown at 24 degrees C revealed that both activities of CO2-dependent oxygen evolution and photosystem II were higher in the parent than in hf-9 in the range between 7 and 40 degrees C. In contrast, hf-9 surpassed the parent in both activities at 45 degrees C. Optimal temperatures for both activities were at around 35 degrees C and 40 degrees C in the parent and hf-9, respectively. Incubation of the cells at 41 and 45 degrees C demonstrated that the activity of photosystem II in hf-9 was more tolerant to the high temperatures than that in the parent. These results suggest that lowered unsaturation levels of chloroplast lipids contributed to high temperature tolerance of photosystem II, and eventually to that of photosynthesis.

    DOI CiNii

  • Photosynthetic characteristics of a mutant of Chlamydomonas reinhardtii impaired in fatty acid desaturation in chloroplasts

    N Sato, K Sonoike, M Tsuzuki, A Kawaguchi

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1274 ( 3 ) 112 - 118  1996年06月  [査読有り]

     概要を見る

    The photosynthetic apparatus of a mutant of Chlamydomonas reinhardtii, hf-9, impaired in fatty acid desaturation at the omega 6 position of fatty acids of chloroplasts was investigated. Measurement of photosynthetic activities revealed that both PS I and PS II activities were reduced in hf-9. However, little alteration occurred in the contents and subunit assemblies of the PS I complex, PS II core complex and light-harvesting complex of PS II. Lipids bound to these chlorophyll-protein (CP) complexes in hf-9 were shown to contain decreased levels of 16:4(4,7,10,13) and 18:3(9,12,15), with accumulation of 16:1(7) and 18:1(9), as compared with in the parent. Highly unsaturated fatty acids of chloroplast lipids may be required for the normal functions of PS I and PS II, by associating with these complexes.

    DOI CiNii

  • Acclimation of respiratory properties of leaves of Spinacia oleracea L, a sun species, and of Alocasia macrorrhiza (L) G. Don, a shade species, to changes in growth irradiance

    K Noguchi, K Sonoike, Terashima, I

    PLANT AND CELL PHYSIOLOGY   37 ( 3 ) 377 - 384  1996年04月  [査読有り]

     概要を見る

    To clarify the way in which the light available for growth affects respiration in leaves of sun and shade plants, we examined the respiratory properties of mature leaves of Spinacia oleracea L., a sun species, and of Alocasia macrorrhiza (L.) G. Don., a shade species, that had been grown at various irradiances. In leaves of S. oleracea, the respiratory rates, on a dry mass basis, decreased with time during the night, and the higher was the growth irradiance during the day, the higher was the respiratory rate. The marked decreases in the respiratory rate during the night were accompanied by decreases in the concentration of carbohydrates in the leaves, By contrast, the respiratory rates of leaves of A. macrorrhiza were virtually constant throughout the night and the absolute rates were lower than those of S. oleracea even though the absolute value of the concentration of carbohydrates and its decrease at night resembled to those in S. oleracea, The maximum activities of respiratory enzymes were also similar to those in S. oleracea. However, the leaves of A. macrorrhiza contained less soluble protein than those of S. oleracea, These results suggest that, in S. oleracea, the concentration of carbohydrates might determine the respiratory rate while such is not the case in A. macrorrhiza. The lower respiratory rates in A. macrorrhiza might be due to a lower demand for ATP.

    DOI

  • Photoinhihition of photosystem I: Its physiological significance in the chilling sensitivity of plants

    K Sonoike

    PLANT AND CELL PHYSIOLOGY   37 ( 3 ) 239 - 247  1996年04月  [査読有り]  [招待有り]

     概要を見る

    Photoinhibition was defined originally as the decrease in photosynthetic activity that occurs upon excess illumination. The site of photoinhibition has generally been considered to be located in PSII. However, a novel type of photoinhibition has recently been characterized in chilling-sensitive plants. This photoinhibition occurs under relatively weak illumination at chilling temperatures and the main site of damage is in PSI. The photoinhibition of PST is initiated by the inactivation of the acceptor side, with the subsequent destruction of the reaction center and the degradation of the product of the psaB gene, which is one of the two major subunit polypeptides of the PSI reaction center complex. Chilling and oxidative stress (the presence of reactive species of oxygen) are characteristic requirements for the photoinhibition of PSI in vivo.

    DOI

  • Stoichiometries of photosystem I and photosystem II in rice mutants differently deficient in chlorophyll b

    T Terao, K Sonoike, JY Yamazaki, Y Kamimura, S Katoh

    PLANT AND CELL PHYSIOLOGY   37 ( 3 ) 299 - 306  1996年04月  [査読有り]

     概要を見る

    Stoichiometries of photosystem I (PSI) and photosystem II (PSII) reaction centers in a cultivar of rice, Norin No, 8, and three chlorophyll b-deficient mutants derived from the cultivar were investigated. Quantitation of PSI by photooxidation of P-700 and chromatographic assay of vitamin K-1 showed that, on the basis of chlorophyll, the mutants have higher concentrations of PSI than the wildtype rice, Greater increases were observed in the PSII contents measured by photoreduction of Q(A), binding of a radioactive herbicide and atomic absorption spectroscopy of Mn. Consequently, the PSII to PSI ratio increased from 1.1-1.3 in the wild-type rice to 1.8 in chlorina 2, which contains no Chi b, and to 2.0-3.3 in chlorina 11 and chlorina 14, which have chlorophyll a/b ratios of 9 and 13, respectively. Measurement of oxygen evolution with saturating single-turnover flashes revealed that, whereas at most 20% of PSII centers are inactive in oxygen evolution in the wildtype rice, the non-functional PSII centers amount to about 50% in the three mutant strains, The fluorescence induction kinetics was also analyzed to estimate proportions of the inactive PSII in the mutants. The data obtained suggest that plants have an ability to adjust the stoichiometry of the two photosystems and the functional organization of PSII in response to the genetically induced deficiency of chlorophyll b.

    DOI

  • Degradation of psaB gene product, the reaction center subunit of photosystem I, is caused during photoinhibition of photosystem I: Possible involvement of active oxygen species

    K Sonoike

    PLANT SCIENCE   115 ( 2 ) 157 - 164  1996年03月  [査読有り]

     概要を見る

    Specific degradation of psaB gene product, one of the two large subunits of photosystem I (PS I) reaction-center, was observed during photoinhibition of PS I. In the in vitro photoinhibition using spinach thylakoid membranes, the degradation of psaB gene product gave rise to fragments of 51 kDa and 45 kDa. n-Propyl gallate, a scavenger of active oxygen species, suppressed the generation of these fragments. Addition of methyl viologen, which protects PS I from photoinhibition but increases the production of superoxide, suppressed the generation of 51 kDa fragment but increased the generation of 45 kDa fragment. It was concluded that interaction of active oxygen species with reduced electron acceptor in PS I results in inactivation of PS I and in generation of 51 kDa fragment. On the other hand, 45 kDa fragment was generated solely by the presence of active oxygen species independent of the inhibition of PS I activity.

    DOI CiNii

  • IMPAIRED PHOTOSYSTEM-II IN A MUTANT OF CHLAMYDOMONAS-REINHARDTII DEFECTIVE IN SULFOQUINOVOSYL DIACYLGLYCEROL

    N SATO, K SONOIKE, M TSUZUKI, A KAWAGUCHI

    EUROPEAN JOURNAL OF BIOCHEMISTRY   234 ( 1 ) 16 - 23  1995年11月  [査読有り]

     概要を見る

    The photosynthetic apparatus was characterized in a mutant of Chlamydomonas reinhardtii, hf-2, defective in the synthesis of a chloroplast-specific lipid, sulfoquinovosyl diacylglycerol (SQui-acyl(2)Gro). hf-2 showed reduced photosystem II (PSII) activity with little effect on photosystem I (PSI) activity, as compared with the parent. PAGE in the presence of dodecyl beta-D-maltoside (DodGlc(2)) of C. reinhardtii thylakoid membranes was used to isolate chlorophyll-protein complexes without chlorophyll (Chl) release in order to examine lipid species bound to these complexes. The four complexes obtained were shown to be the PSI complex, the PSII core complex and the two groups of the light-harvesting complex of PSII by analyses of 77-K emission spectra of Chl fluorescence and of subunit compositions. Lipid analysis of Chl-protein complexes in the parent revealed the localization of SQui-acyl(2)Gro in the PSII core complex and the two groups of the light-harvesting complex of PSII, but not in the PSI complex. These results suggest that SQui-acyl(2)Gro is responsible for PSII activity by associating with the core and light-harvesting complexes of PSII.

    DOI PubMed CiNii

  • SELECTIVE PHOTOINHIBITION OF PHOTOSYSTEM-I IN ISOLATED THYLAKOID MEMBRANES FROM CUCUMBER AND SPINACH

    K SONOIKE

    PLANT AND CELL PHYSIOLOGY   36 ( 5 ) 825 - 830  1995年07月  [査読有り]

     概要を見る

    The site of photoinhibition at low temperatures in leaves of a chilling-sensitive plant, cucumber, is photosystem I [Terashima et al. (1994) Planta 193: 300]. As described herein, selective photoinhibition of PSI can also be induced in isolated thylakoid membranes in vitro. Inhibition was observed both at chilling temperatures and at 25 degrees C, and not only in the thylakoid membranes isolated from cucumber, but also in those isolated from a chilling-tolerant plant, spinach. Comparison of these observations in vitro to the earlier results in vivo indicates that (1) photoinhibition of PSI is a universal phenomenon; (2) a mechanism exists to protect PSI in vivo; and (3) the protective mechanism is chilling-sensitive in cucumber. The chilling-sensitive component seems to be lost during the isolation of thylakoid membranes. Very weak light (10-20 mu umol m(-2) s(-1)) was sufficient to cause the inhibition of PSI. About 80% of the oxygen-evolving activity by PSII was maintained even after the activity of PSI had decreased by more than 70%. This is the first report of the selective photoinhibition of PSI in vitro.

    DOI

  • DESTRUCTION OF PHOTOSYSTEM-I IRON-SULFUR CENTERS IN LEAVES OF CUCUMIS-SATIVUS L BY WEAK ILLUMINATION AT CHILLING TEMPERATURES

    K SONOIKE, TERASHIMA, I, M IWAKI, S ITOH

    FEBS LETTERS   362 ( 2 ) 235 - 238  1995年04月  [査読有り]

     概要を見る

    The activity of photosystem (PS) I in cucumber leaves was selectively inhibited by weak illumination at chilling temperatures with almost no loss of P-700 content and PSII activity, The sites of inactivation in the reducing side of PSI were determined by EPR and flash photolysis, Measurement by EPR showed the destruction of iron-sulfur centers, F-X, F-A and F-B, in parallel with the loss of quantum yield of electron transfer from diaminodurene to NADP(+). Flash photolysis showed the increases in the triplet states of P-700 and antenna pigments, along with the decrease in the electron transfer from P-700 to F-A/F-B. This indicates the increase in the charge recombination between P-700(+) and A(0)(-). It is concluded that weak-light treatment of cucumber leaves at chilling temperature destroys F-X, F-A and F-B and possibly A(1). This gives the molecular basis for the mechanism of selective PSI photodamage that was recently reported [Sonoike and Terashima (1994) Planta 194, 287-293].

    DOI PubMed CiNii

  • Contribution of lipids to PSII

    N Sato, K Sonoike, M Tsuzuki, A Kawaguchi

    PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL III     989 - 992  1995年  [査読有り]

  • Chilling sensitive steps in leaves of Phaseolus vulgaris L examination of the effects of growth irradiances on PSI photoinhibition

    K Sonoike, M Ishibashi, A Watanabe

    PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL IV     853 - 856  1995年  [査読有り]

  • The loss of RuBPCase by 24-hour treatment of ''rain'' in light

    M Ishibashi, Terashima, I, H Usuda, K Sonoike, A Watanabe

    PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE, VOL IV     617 - 620  1995年  [査読有り]

  • THE PSAC PROTEIN IS NECESSARY FOR THE STABLE ASSOCIATION OF THE PSAD, PSAE, AND PSAL PROTEINS IN THE PHOTOSYSTEM-I COMPLEX - ANALYSIS OF A CYANOBACTERIAL MUTANT STRAIN

    RM MANNAN, HB PAKRASI, K SONOIKE

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   315 ( 1 ) 68 - 73  1994年11月  [査読有り]

     概要を見る

    The PsaC protein binds two 4Fe-4S centers, F-A and F-B, in the photosystem I (PSI) protein complex. In the T398 strain of Anabaena variabilis ATCC 29413, the psaC gene encoding this protein has been insertionally inactivated by the introduction of a neomycin resistance gene cartridge in the coding region. Photosystem I complex was purified through native gel electrophoresis of beta-dodecyl maltoside solubilized thylakoid membranes from wild-type and T398 strains of Anabaena 29413. The PSI complex from T398 strain retained functionally active P700, the reaction center chlorophylls. Interestingly, purified PSI complex from T398 cells lacked the PsaD, PsaE, and PsaL polypeptides. Western analysis with polyclonal antibodies raised against these proteins indicated that the two stromal exposed polypeptides, PsaD and PsaE, are absent in isolated thylakoid membranes from T398 cells. The PsaL polypeptide could be detected at a level comparable to that in wild-type thylakoid membranes, although it is absent in the PSI preparation from the mutant. These observations suggest that the PsaC protein is essential for the stable association of PsaD and PsaE, two hydrophilic, extrinsic polypeptides. Moreover, PsaL, a hydrophobic protein is loosely associated with PSI and is lost during the isolation of the PSI complex. (C) 1994 Academic Press, Inc.

  • MECHANISM OF PHOTOSYSTEM-I PHOTOINHIBITION IN LEAVES OF CUCUMIS-SATIVUS L

    K SONOIKE, TERASHIMA, I

    PLANTA   194 ( 2 ) 287 - 293  1994年07月  [査読有り]

     概要を見る

    It was recently shown that the site of photoinhibition in leaves of Cucumis sativus L. at low temperatures is Photosystem I (PSI), not PSII (I. Terashima et al. 1994, Planta 193, 300-306). In the present study, the mechanisms of this PSI photoinhibition in vivo were examined. By lowering the photon flux density during the photoinhibitory treatment of leaves at 4 degrees C for 5 h to less than 100 mu mol.m(-2).S-1, We were able to separate the steps of the destruction of the electron-transfer components. Although P-700, the reaction-center chlorophyll, was almost intact in this low-light treatment, the quantum yield of the electron transfer through PSI and photochemically induced absorption change at 701 nm were markedly inhibited. This, along with the results from the measurements of the light-induced absorption changes in the presence of various concentrations of methyl viologen, an artificial electron acceptor, indicates that the component on the acceptor side of the PSI, A(1) or F-x, is the first site of inactivation. When the photon flux density during the treatment was increased to 220 mu mol.m(-2)s(-1), the destruction of P-700 itself was also observed. Furthermore, the partial degradation of the chlorophyll-binding large subunits was observed in photoinhibited leaves. This degradation of the subunits was not detected when the treatment was carried out under nitrogen atmosphere, the condition in which the electron transfer is not inhibited. Thus, the photoinhibitory processes in the reaction center of PSI go through three steps, the inactivation of the acceptor side, the destruction of the reaction-center chlorophyll and the degradation of the reaction center subunit(s). The similarities and the differences between the mechanisms of PSI photoinhibition and those of PSII photoinhibition are discussed.

    DOI CiNii

  • NUCLEOTIDE-SEQUENCES OF THE PSAA AND THE PSAB GENES ENCODING THE REACTION-CENTER PROTEINS OF PHOTOSYSTEM-I IN ANABAENA-VARIABILIS ATCC-29423

    KJ NYHUS, K SONOIKE, HB PAKRASI

    BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS   1185 ( 2 ) 247 - 251  1994年04月  [査読有り]

     概要を見る

    We have determined the nucleotide sequence of the psaAB gene cluster from the filamentous cyanobacterium Anabaena variabilis ATCC 29413. These genes encode the Photosystem I reaction center proteins, which are highly homologous to similar proteins in other cyanobacteria and higher plants.

    DOI PubMed CiNii

  • THE SITE OF PHOTOINHIBITION IN LEAVES OF CUCUMIS-SATIVUS L AT LOW-TEMPERATURES IS PHOTOSYSTEM-I, NOT PHOTOSYSTEM-II

    TERASHIMA, I, S FUNAYAMA, K SONOIKE

    PLANTA   193 ( 2 ) 300 - 306  1994年03月  [査読有り]

     概要を見る

    Maximum quantum yields (QY) of photosynthetic electron flows through PSI and PSII were separately assessed in thylakoid membranes isolated from leaves of Cucumis sativus L. (cucumber) that had been chilled in various ways. The QY(PSI) in the thylakoids prepared from the leaves treated at 4-degrees-C in moderate light at 220 mumol quanta . m-2.S-1 (400 700 nm) for 5 h, was about 20 30% of that in the thylakoids prepared from untreated leaves, while QY(PSII) decreased, at most, by 20% in response to the same treatment. The decrease in QY(PSI) was observed only when the leaves were chilled at temperatures below 10-degrees-C, while such a marked temperature dependency was not observed for the decrease in QY(PSII). In the chilling treatment at 4-degrees-C for 5 h, the quantum flux density that was required to induce 50% loss of QY(PSI) was ca. 50 mumol quanta m-2.s-1. When the chilling treatment at 4-degrees-C in the light was conducted in an atmosphere of N2, photoinhibition of PSI was largely suppressed, while the damage to PSII was somewhat enhanced. The ferricyanide-oxidised minus ascorbate-reduced difference spectra and the light-induced absorbance changes at 700 nm obtained with the thylakoid suspension, indicated the loss of P700 to extents that corresponded to the decreases in QY(PSI). Accordingly, the decreases in QY (PSI) can largely be attributed to destruction of the PSI reaction centre itself. These results clearly show that, at least in cucumber, a typical chilling-sensitive plant, PSI is much more susceptible to aerobic photoinhibition than PSII.

    DOI

  • The PsaC protein is necessary for the stable association of the PsaD, PsaE, and PsaL proteins in the photosystem i complex: Analysis of a cyanobacterial mutant strain

    R. Mannar Mannan, Himadri B. Pakrasi, Kintake Sonoike

    Archives of Biochemistry and Biophysics   315 ( 1 ) 68 - 73  1994年  [査読有り]

     概要を見る

    The PsaC protein binds two 4Fe-4S centers, FA and FB, in the photosystem I (PSI) protein complex. In the T398 strain of Anabaena variabilis ATCC 29413, the psaC gene encoding this protein has been insertionally inactivated by the introduction of a neomycin resistance gene cartridge in the coding region. Photosystem I complex was purified through native gel electrophoresis of β-dodecyl maltoside solubilized thylakoid membranes from wild-type and T398 strains of Anabaena 29413. The PSI complex from T398 strain retained functionally active P700, the reaction center chlorophylls. Interestingly, purified PSI complex from T398 cells lacked the PsaD, PsaE, and PsaL polypeptides. Western analysis with polyclonal antibodies raised against these proteins indicated that the two stromal exposed polypeptides, PsaD and PsaE, are absent in isolated thylakoid membranes from T398 cells. The PsaL polypeptide could be detected at a level comparable to that in wild-type thylakoid membranes, although it is absent in the PSI preparation from the mutant. These observations suggest that the PsaC protein is essential for the stable association of PsaD and PsaE, two hydrophilic, extrinsic polypeptides. Moreover, PsaL, a hydrophobic protein is loosely associated with PSI and is lost during the isolation of the PSI complex. © 1994 Academic Press.

    DOI PubMed CiNii

  • CHEMICAL ENVIRONMENT HETEROGENEITY AROUND THE 2 CHLOROPHYLL-A' MOLECULES IN PHOTOSYSTEM-I

    H MAEDA, T WATANABE, K SONOIKE

    JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY   20 ( 2-3 ) 139 - 143  1993年10月  [査読有り]

     概要を見る

    Treatment of whole and fractionated plant tissues with hydrophilic and hydrophobic solvent mixtures of varied volume ratio liberates two chlorophyll (Chl) a' molecules from the photosystem (PS)I core when the solvent hydrophilicity exceeds a critical level, whereas only one molecule is extracted in hydrophobic media. The PSI core proteins, PSI-A and PSI-B, which form a heterodimer, appear to bind one Chl a' molecule each, in local environments significantly different regarding their hydrophilicity or hydrophobicity.

    DOI

  • Small subunits of Photosystem I reaction center complexes from Synechococcus elongatus. I. Is the psaF gene product required for oxidation of cytochrome c-553?

    Hideki Hatanaka, Kintake Sonoike, Masahiko Hirano, Sakae Katoh

    BBA - Bioenergetics   1141 ( 1 ) 45 - 51  1993年02月  [査読有り]

     概要を見る

    Photosystem I (PS I) reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus with nonionic detergents, digitonin or sucrose monolaurate, contained eight small subunit polypeptides. Two of the small polypeptides were identified by analysis of their N-terminal amino-acid sequences as the psaF and psaE gene products. Treatment with a cationic detergent, cetyltrimethylammonium bromide, resulted in depletion of five small subunits including the psaF gene product. Five PS I complexes isolated with an anionic detergent, sodium dodecylsulfate, contained zero to four small subunits but were all depleted of the psaF polypeptide. The function of the psaF gene product was examined by measuring reduction kinetics of flash-oxidized P-700 in the presence of different concentrations of cytochrome c-553. Oxidized P-700 was rapidly reduced by the reduced cytochrome in all the PS I complexes that contained, at least, the psaC and psaD polypeptides and the second-order rate constants of electron transfer from cytochrome c-553 to P-700 were essentially the same between PS I complexes that contained the psaF polypeptide and those that lost this polypeptide. Thus, the psaF polypeptide is not required for the bimolecular reaction between P-700 and cytochrome c-553. Mg2+ had a moderate stimulating effect on the rate of P-700 reduction whether PS I complexes were associated with the psaF gene product or not. The function of this subunit polypeptide is discussed. © 1993.

    DOI PubMed CiNii

  • Small subunits of Photosystem I reaction center complexes from Synechococcus elongatus. II. The psaE gene product has a role to promote interaction between the terminal electron acceptor and ferredoxin

    Kintake Sonoike, Hideki Hatanaka, Sakae Katoh

    BBA - Bioenergetics   1141 ( 1 ) 52 - 57  1993年02月  [査読有り]

     概要を見る

    Function of a subunit polypeptide (the psaE gene product) of Photosystem I (PS I) reaction center complexes was investigated by comparing the reactivity of the reduced iron-sulfur centers (FA/FB)- with ferredoxin among Synechococcus PS I complexes which had been variously depleted of this polypeptide. Ferredoxin at or below 1 μM can accept electrons from (FA/FB)- effectively competing with the back reaction between P-700+ and (FA/FB)- in the thylakoid membranes and PS I complexes that contained all the eight small subunits. The high reactivity of (FA/FB)- with low concentrations of ferredoxin was observed in PS I complexes which contain only the products of psaC, psaD and psaE genes but not in complexes which carry the psaC, psaD, psaL and psaK gene products but no psaE gene product. Varied amounts of the psaE gene product were extracted by treatment with different concentrations of a cationic detergent, dodecyltrimethylammonium bromide, and 2.5 M NaCl. The solubilized polypeptide was then reconstituted to the depleted complexes. The magnitudes of the back reaction that could be suppressed by addition of ferredoxin at or below 1 μM were well correlated to the amounts of the psaE polypeptide remained bound or rebound to the complexes. It is concluded that the product of the psaE gene has a role to promote the interaction between the terminal bound electron acceptor and ferredoxin. A high autooxidizability of (FA/FB)- and contrasting effects of lipophilic cations and anions on the rate of the back reaction from (FA/FB)- to P-700+ were also reported. © 1993.

    DOI PubMed CiNii

  • Thermoluminescence emission at liquid helium temperatures from photosynthetic apparatus and purified pigments

    Takumi Noguchi, Yorinao Inoue, Kintake Sonoike

    BBA - Bioenergetics   1141 ( 1 ) 18 - 22  1993年02月  [査読有り]

     概要を見る

    Thermoluminescence (TL) emission below 77 K from photosynthetic pigment protein complexes and purified pigments was examined using liquid He. At least four TL components emitting at around 20, 50, 70 and 90 K were resolved on the glow curve from thylakoids. The 20, 50 and 70 K bands are newly observed TL components and designated as Zα, Zβ and Zγ bands, respectively. The 90 K band was found to be a different expression of the well-know Z band which was reported as the 110 K band in literatures. These TL bands were evidenced not to be related with charge separation and subsequent electron transfer in reaction centers but originate from light-harvesting chlorophyll (Chl) a and b by the following observations: (1) red light, which causes charge separation in reaction centers, was ineffective in inducing these TL components
    (2) isolated LHCI and LHCII showed higher TL intensities than isolated PS I core and PS II core complexes
    and (3) purified Chl a and Chl b in solid state exhibited essentially the same TL bands. Chl-Chl and Chl-ligand interactions in proteins have been discussed as possible chemical identities of the energy trap responsible for the TL bands. © 1993.

    DOI CiNii

  • SMALL SUBUNITS OF PHOTOSYSTEM-I REACTION CENTER COMPLEXES FROM SYNECHOCOCCUS-ELONGATUS .2. THE PSAE GENE-PRODUCT HAS A ROLE TO PROMOTE INTERACTION BETWEEN THE TERMINAL ELECTRON-ACCEPTOR AND FERREDOXIN

    K SONOIKE, H HATANAKA, S KATOH

    BIOCHIMICA ET BIOPHYSICA ACTA   1141 ( 1 ) 52 - 57  1993年02月  [査読有り]

     概要を見る

    Function of a subunit polypeptide (the psaE gene product) of Photosystem I (PS I) reaction center complexes was investigated by comparing the reactivity of the reduced iron-sulfur centers (F(A)/F(B))- with ferredoxin among Synechococcus PS I complexes which had been variously depleted of this polypeptide. Ferredoxin at or below 1 muM can accept electrons from (F(A)/F(B))- effectively competing with the back reaction between P-700+ and (F(A)/F(B))- in the thylakoid membranes and PS I complexes that contained all the eight small subunits. The high reactivity of (F(A)/F(B))- with low concentrations of ferredoxin was observed in PS I complexes which contain only the products of psaC, psaD and psaE genes but not in complexes which carry the psaC, psaD, psaL and psaK gene products but no psaE gene product. Varied amounts of the psaE gene product were extracted by treatment with different concentrations of a cationic detergent, dodecyltrimethylammonium bromide, and 2.5 M NaCl. The solubilized polypeptide was then reconstituted to the depleted complexes. The magnitudes of the back reaction that could be suppressed by addition of ferredoxin at or below 1 muM were well correlated to the amounts of the psaE polypeptide remained bound or rebound to the complexes. It is concluded that the product of the psaE gene has a role to promote the interaction between the terminal bound electron acceptor and ferredoxin. A high autooxidizability of (F(A)/F(B))- and contrasting effects of lipophilic cations and anions on the rate of the back reaction from (F(A)/F(B))- to P-700+ were also reported.

  • SMALL SUBUNITS OF PHOTOSYSTEM-I REACTION CENTER COMPLEXES FROM SYNECHOCOCCUS-ELONGATUS .1. IS THE PSAF GENE-PRODUCT REQUIRED FOR OXIDATION OF CYTOCHROME-C-553

    H HATANAKA, K SONOIKE, M HIRANO, S KATOH

    BIOCHIMICA ET BIOPHYSICA ACTA   1141 ( 1 ) 45 - 51  1993年02月  [査読有り]

     概要を見る

    Photosystem I (PS I) reaction center complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus with nonionic detergents, digitonin or sucrose monolaurate, contained eight small subunit polypeptides. Two of the small polypeptides were identified by analysis of their N-terminal amino-acid sequences as the psaF and psaE gene products. Treatment with a cationic detergent, cetyltrimethylammonium bromide, resulted in depletion of five small subunits including the psaF gene product. Five PS I complexes isolated with an anionic detergent, sodium dodecylsulfate, contained zero to four small subunits but were all depleted of the psaF polypeptide. The function of the psaF gene product was examined by measuring reduction kinetics of flash-oxidized P-700 in the presence of different concentrations of cytochrome c-553. Oxidized P-700 was rapidly reduced by the reduced cytochrome in all the PS I complexes that contained, at least, the psaC and psaD polypeptides and the second-order rate constants of electron transfer from cytochrome c-553 to P-700 were essentially the same between PS I complexes that contained the psaF polypeptide and those that lost this polypeptide. Thus, the psaF polypeptide is not required for the bimolecular reaction between P-700 and cytochrome c-553. Mg2+ had a moderate stimulating effect on the rate of P-700 reduction whether PS I complexes were associated with the psaF gene product or not. The function of this subunit polypeptide is discussed.

  • THERMOLUMINESCENCE EMISSION AT LIQUID-HELIUM TEMPERATURES FROM PHOTOSYNTHETIC APPARATUS AND PURIFIED PIGMENTS

    T NOGUCHI, Y INOUE, K SONOIKE

    BIOCHIMICA ET BIOPHYSICA ACTA   1141 ( 1 ) 18 - 22  1993年02月  [査読有り]

     概要を見る

    Thermoluminescence (TL) emission below 77 K from photosynthetic pigment protein complexes and purified pigments was examined using liquid He. At least four TL components emitting at around 20, 50, 70 and 90 K were resolved on the glow curve from thylakoids. The 20, 50 and 70 K bands are newly observed TL components and designated as Z(alpha), Z(beta) and Z(gamma) bands, respectively. The 90 K band was found to be a different expression of the well-known Z band which was reported as the 110 K band in literatures. These TL bands were evidenced not to be related with charge separation and subsequent electron transfer in reaction centers but originate from light-harvesting chlorophyll (Chl) a and b by the following observations: (1) red light, which causes charge separation in reaction centers, was ineffective in inducing these TL components; (2) isolated LHCI and LHCII showed higher TL intensities than isolated PS I core and PS II core complexes; and (3) purified Chl a and Chl b in solid state exhibited essentially the same TL bands. Chl-Chl and Chl-ligand interactions in proteins have been discussed as possible chemical identities of the energy trap responsible for the TL bands.

  • A NOVEL 3.5 KDA PROTEIN-COMPONENT OF CYANOBACTERIAL PHOTOSYSTEM-I COMPLEXES

    M IKEUCHI, K SONOIKE, H KOIKE, HB PAKRASI, Y INOUE

    PLANT AND CELL PHYSIOLOGY   33 ( 8 ) 1057 - 1063  1992年12月  [査読有り]

     概要を見る

    A novel protein component of 3.5 kDa was detected in photosystem I complexes prepared from several cyanobacteria, viz. Synechococcus vulcanus, Synechococcus elongatus BP-1, Synechococcus sp. PCC 7002 and Synechocystis sp. PCC 6803. The complete amino acid sequence of this component was determined by direct protein sequencing. The sequences of the 3.5 kDa proteins from these four organisms were highly homologous to each other, featuring a hydrophobic domain in the middle. The cyanobacterial consensus sequence exhibits significant homology to the presumed product of ORF32 in the chloroplast DNA of liverwort (Marchantia polymorpha), but no homologous ORF is present in the chloroplast DNA of tobacco or rice. Since this protein appears to interact strongly with the PS I reaction center complex, it may play some role in the function and maintenance of the structure of PS I.

    DOI

  • PRESENCE OF AN N-TERMINAL PRESEQUENCE IN THE PSAI PROTEIN OF THE PHOTOSYSTEM-I COMPLEX IN THE FILAMENTOUS CYANOBACTERIUM ANABAENA-VARIABILIS ATCC-29413

    K SONOIKE, M IKEUCHI, HB PAKRASI

    PLANT MOLECULAR BIOLOGY   20 ( 5 ) 987 - 990  1992年12月  [査読有り]

     概要を見る

    The psaI gene encoding the 5.2 kDa protein component (PsaI) of the photosystem I complex was cloned from the cyanobacterium Anabaena 29413. The gene is present in single copy in this cyanobacterial genome. The nucleotide sequence of a 500 bp region of the cloned DNA revealed the presence of an open reading frame encoding a 46 amino acid long polypeptide. The N-terminal 11 residues are absent in the mature polypeptide and thus represents the first identified cleavable presequence on the PsaI protein. We suggest that this presequence directs the N-terminus of the protein to the thylakoid lumen.

    DOI

  • AN APPLICATION OF A 2-DIMENSIONAL PHOTONCOUNTER FOR THE DETERMINATION OF EMISSION-SPECTRA OF THERMOLUMINESCENCE FROM PHOTOSYNTHETIC SYSTEMS

    K SONOIKE, Y INOUE

    JOURNAL OF LUMINESCENCE   51 ( 1-3 ) 129 - 137  1992年02月  [査読有り]

     概要を見る

    Emission spectra of two components of thermoluminescence from photosynthetic systems were measured using a two-dimensional photoncounter. Application of this detector system enormously improved the signal to noise (S/N) ratio and enabled the determination of an emission spectrum of a specific thermoluminescence component. The B-band emitting at 30-degrees-C showed an emission spectrum having a single peak at 690 nm, whereas the Z-band emitting at -160-degrees-C showed a spectrum having two peaks, the major band at 740 nm and a satellite band at 690 nm. It was revealed that the longer wavelength component arises from photosystem I and the shorter wavelength component from photosystem II. Light harvesting chlorophyll a/b protein complexes (LHCI and LHCII) were also found to emit the Z-band at 740 and 690 nm, respectively. The advantage of a two-dimensional photoncounter for the determination of emission spectra of weak luminescence from large-surface biological samples is discussed.

    DOI CiNii

  • EXPOSURE OF LEAVES OF CUCUMIS-SATIVUS L TO LOW-TEMPERATURES IN THE LIGHT CAUSES UNCOUPLING OF THYLAKOIDS .2. NONDESTRUCTIVE MEASUREMENTS WITH INTACT LEAVES

    TERASHIMA, I, K SONOIKE, T KAWAZU, S KATOH

    PLANT AND CELL PHYSIOLOGY   32 ( 8 ) 1275 - 1283  1991年12月  [査読有り]

     概要を見る

    To examine the effects of chilling of leaves of cucumber (Cucumis sativus L.) in moderate light on the coupling state of thylakoids in situ, changes in fluorescence, changes in light scattering and flash-induced changes in absorbance at 518 nm were examined in intact leaves. After chilling of leaves at 5-degrees-C in the light for 5 h, the non-photochemical quenching of fluorescence, a measure of energisation of thylakoids, was largely suppressed. The treatment also caused a suppression of light-induced changes in the light scattering by leaves, which depends on the formation of a pH gradient across thylakoid membranes. When thylakoids were prepared by very gentle methods from the leaves chilled in the light, through a step of preparation of intact chloroplasts, the transport of electrons from H2O to ferricyanide was uncoupled, being insensitive to an uncoupler, methylamine.
    These data provide consistent evidence that the thylakoids are uncoupled in situ by the chilling of leaves in the light and, as a consequence of the uncoupling, the energisation of the membranes is suppressed. However, the decay of the flash-induced change in absorbance at 518 nm in leaves was not markedly accelerated by the treatment. The thylakoids isolated from leaves chilled in the light, which were in the uncoupled state, also did not show a rapid decay, unless an efficient uncoupler such as gramicidin was added. These results suggest that even a considerable uncoupling of thylakoids, brought about by chilling of leaves in the light, is not sufficient to cause a marked acceleration of the decay of the flash-induced change in absorbance at 518 nm. Therefore, analysis at 518 nm is not always a sensitive method for assessing the coupling state of thylakoids.

    DOI

  • TOTAL IMMOBILIZATION OF THE EXTRINSIC 33-KDA PROTEIN IN SPINACH PHOTOSYSTEM-II MEMBRANE PREPARATIONS - PROTEIN STOICHIOMETRY AND STABILIZATION OF OXYGEN EVOLUTION

    ENAMI, I, M KANEKO, N KITAMURA, H KOIKE, K SONOIKE, Y INOUE, S KATOH

    BIOCHIMICA ET BIOPHYSICA ACTA   1060 ( 2 ) 224 - 232  1991年10月  [査読有り]

     概要を見る

    (1) Treatment of oxygen-evolving Photosystem II membrane fragments (PS II membranes) with a zero-length crosslinker, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) led to immobilization of all the extrinsic 33 kDa protein molecules without any significant effects on the oxygen-evolving activity and oscillation patterns of flash-induced oxygen evolution and thermoluminescence B band. (2) With increasing concentration of EDC, the chlorophyll-binding 47 kDa protein decreased in parallel with the 33 kDa protein, yielding a crosslinked product consisting of one each of the two proteins. The results, which indicate that the two proteins are present in equimolar amounts in PS II membranes, are consistent with the stoichiometry of one copy of the 33 kDa protein per PS II unit. (3) The total immobilization of the 33 kDa protein stabilized 40 to 60% of the oxygen-evolving activity against urea/NaCl-, CaCl2- and MgCl2-wash, which otherwise solubilize the three extrinsic proteins and strongly inactivate oxygen evolution. The result implies that extraction of the extrinsic proteins may not be the sole cause of the inactivation of oxygen evolution by these treatments. (4) The crosslinking of the 33 kDa protein with EDC had no protecting effect against Tris-, NH2OH- and pH 9.0-treatments. However, the stability of oxygen evolution at alkaline pH levels was slightly but significantly increased by treatment of PS II membranes with dithiobis(succinimidylpropionate), which specifically modifies amino groups.

  • The emission spectra of thermoluminescence from the photosynthetic apparatus

    Kintake Sonoike, Hiroyuki Koike, Isao Enami, Yorinao Inoue

    BBA - Bioenergetics   1058 ( 2 ) 121 - 130  1991年06月  [査読有り]

     概要を見る

    Emission spectra of two thermoluminescence (TL) components emitted from the photosynthetic apparatus were determined by the use of an imaging photon detector system. The following results were obtained: (i) The TL B-band emitted from whole thylakoids and PS II core complex showed the same emission spectrum peaking at about 690 nm (uncorrected for wavelength dependence of the photocathode) with a broad tailing in far-red region. This spectrum agrees with the reported emission spectrum of delayed luminescence, consistent with the view that the TL B-band arises from thermally stimulated recombination of charge pairs in PS II. (ii) The emission spectrum of TL Z-band was variant, depending on the PS I/PS II ratio in the sample. This was because the Z-band consists of two spectral components emitting at about 740 nm and 690 nm (also uncorrected for the wavelength dependence of the photocathode)
    the former originates exclusively from PS I and the latter from PS II. (iii) Light-harvesting chlorophyll protein complexes (LHCI and LHCII) emit the respective spectral components of Z-band more strongly than do their respective core complexes, indicating that reaction center photochemistry is not involved in energy storage for the Z-band. (iv) A methanol extract of thylakoids emitted only a weak Z-band at 690 nm, but the formation of a chlorophyll aggregate markedly enhanced the Z-band intensity, concomitant with a shift in emission maximum to 740 nm. The mechanism of energy storage for Z-band is discussed in relation to the local chlorophyll concentration. © 1991 Elsevier Science Publishers B.V. All rights reserved.

    DOI CiNii

  • THE EMISSION-SPECTRA OF THERMOLUMINESCENCE FROM THE PHOTOSYNTHETIC APPARATUS

    K SONOIKE, H KOIKE, ENAMI, I, Y INOUE

    BIOCHIMICA ET BIOPHYSICA ACTA   1058 ( 2 ) 121 - 130  1991年06月  [査読有り]

     概要を見る

    Emission spectra of two thermoluminescence (TL) components emitted from the photosynthetic apparatus were determined by the use of an imaging photon detector system. The following results were obtained: (i) The TL B-band emitted from whole thylakoids and PS 11 core complex showed the same emission spectrum peaking at about 690 nm (uncorrected for wavelength dependence of the photocathode) with a broad tailing in far-red region. This spectrum agrees with the reported emission spectrum of delayed luminescence, consistent with the view that the TL B-band arises from thermally stimulated recombination of charge pairs in PS II. (ii) The emission spectrum of TL Z-band was variant, depending on the PS I/PS II ratio in the sample. This was because the Z-band consists of two spectral components emitting at about 740 nm and 690 nm (also uncorrected for the wavelength dependence of the photocathode); the former originates exclusively from PS I and the latter from PS II. (iii) Light-harvesting chlorophyll protein complexes (LHCI and LHCII) emit the respective spectral components of Z-band more strongly than do their respective core complexes, indicating that reaction center photochemistry is not involved in energy storage for the Z-band. (iv) A methanol extract of thylakoids emitted only a weak Z-band at 690 nm, but the formation of a chlorophyll aggregate markedly enhanced the Z-band intensity, concomitant with a shift in emission maximum to 740 nm. The mechanism of energy storage for Z-band is discussed in relation to the local chlorophyll concentration.

  • Total immobilization of the extrinsic 33 kDa protein in spinach Photosystem II membrane preparations. Protein stoichiometry and stabilization of oxygen evolution

    Isao Enami, Mari Kaneko, Nobuhito Kitamura, Hiroyuki Koike, Kintake Sonoike, Yorinao Inoue, Sakae Katoh

    Biochimica et Biophysica Acta - Bioenergetics   1060 ( 2 ) 224 - 232  1991年  [査読有り]

     概要を見る

    (1) Treatment of oxygen-evolving Photosystem II membrane fragments (PS II membranes) with a zero-length crosslinker, l-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) led to immobilization of all the extrinsic 33 kDa protein molecules without any significant effects on the oxygen-evolving activity and oscillation patterns of flash-induced oxygen evolution and thermoluminescence B band. (2) With increasing concentration of EDC, the chlorophyll-binding 47 kDa protein decreased in parallel with the 33 kDa protein, yielding a crosslinked product consisting of one each of the two proteins. The results, which indicate that the two proteins are present in equimolar amounts in PS II membranes, are consistent with the stoichiometry of one copy of the 33 kDa protein per PS II unit. (3) The total immobilization of the 33 kDa protein stabilized 40 to 60% of the oxygen-evolving activity against urea/NaCl−, CaCl2− and MgCl2-wash, which otherwise solubilize the three extrinsic proteins and strongly inactivate oxygen evolution. The result implies that extraction of the extrinsic proteins may not be the sole cause of the inactivation of oxygen evolution by these treatments. (4) The crosslinking of the 33 kDa protein with EDC had no protecting effect against Tris-, NH2OH- and pH 9.0-treatments. However, the stability of oxygen evolution at alkaline pH levels was slightly but significantly increased by treatment of PS II membranes with dithiobis(suc-cinimidylpropionate), which specifically modifies amino groups. © 1991, Elsevier Science Publishers B.V. All rights reserved. All rights reserved.

    DOI CiNii

  • VARIATIONS OF THE DIFFERENTIAL EXTINCTION COEFFICIENT OF P-700 AND REESTIMATION OF STOICHIOMETRY OF CONSTITUENTS IN PHOTOSYSTEM-I REACTION CENTER COMPLEXES FROM SYNECHOCOCCUS-ELONGATUS

    K SONOIKE, S KATOH

    CURRENT RESEARCH IN PHOTOSYNTHESIS, VOLS 1-4     B595 - B598  1990年  [査読有り]

  • Heat-Stability of Iron Sulfur Centers and P-700 in Photosystem I Reaction Center Complexes Isolafed from the Thermophilic Cyanobacterium Synechococcus elongatus

    Sonoike, K, Hatanaka, H, Katoh, S, Itoh, S

    Plant and Cell Physiology   31 ( 6 ) 865 - 870  1990年01月  [査読有り]

    DOI

  • Variation and Estimation of the Differential Absorption Coefficient of P-700 in Spinach Photosystem I Preparations

    Sonoike, K, Katoh, S

    Plant and Cell Physiology   31 ( 8 ) 1079 - 1082  1990年01月  [査読有り]

    DOI

  • SIMPLE ESTIMATION OF THE DIFFERENTIAL ABSORPTION-COEFFICIENT OF P-700 IN DETERGENT-TREATED PREPARATIONS

    K SONOIKE, S KATOH

    BIOCHIMICA ET BIOPHYSICA ACTA   976 ( 2-3 ) 210 - 213  1989年09月  [査読有り]

    DOI

  • Effects of sodium dodecyl sulfate and methyl viologen on the differential extinction coefficient of P-700 - a band shift of chlorophyll a associated with oxidation of P-700

    Kintake Sonoike, Sakae Katoh

    BBA - Bioenergetics   935 ( 1 ) 61 - 71  1988年08月  [査読有り]

     概要を見る

    The magnitude of flash-induced bleaching at 700 nm in the thylakoid membranes isolated from the thermophilic cyanobacterium Synechococcus sp. was affected neither by addition of 4 mM methyl viologen, nor by treatment of the membranes with 0.1% sodium dodecyl sulfate (SDS) for 1 h, but appreciably increased on addition of methyl viologen to the SDS-treated membranes. Detailed studies on the light-minus-dark difference spectrum of P-700 revealed that methyl viologen induces a shift-type change consisting of a bleaching at 695 nm and a positive band at 685 nm, and consequently increases the magnitude of the 700 nm bleaching without affecting P-700 photooxidation in the SDS-treated membranes. Other bipyridinium dyes, 1,1′-trimethylene-2,2′-bipyridinium dibromide and 1,1′-trimethylene-5,5′-dimethyl-2,2′-bipyridinium dibromide were equally effective as methyl viologen, and a prolonged treatment of the membranes with SDS caused a similar band shift in the difference spectrum of P-700. The band shift is closely associated with P-700 oxidation because, on redox titration, the magnitude of the band shift varied in parallel to the amount of P-700 oxidized by light. Methyl viologen also induced the band shift in the chemically oxidized-minus-reduced difference spectrum of P-700 in the SDS-treated membranes. Thus, the band shift is not related to reduction of a bound electron acceptor. As a consequence of the band shift, the oxidized-minus-reduced differential extinction coefficient of P-700 in the 700 nm region was increased by 40%. The extinction coefficient was 64 mM-1 · cm-1 at 701 nm in the thylakoid membranes, whereas the 1-h treated membranes with added methyl viologen, or a Photosystem I reaction center complex prepared by SDS-gel electrophoresis, showed the extinction value of 84-86 mM-1 · cm-1 at 698 nm. Two different models for the band shift are discussed. © 1988.

    DOI CiNii

  • EFFECTS OF SODIUM DODECYL-SULFATE AND METHYL VIOLOGEN ON THE DIFFERENTIAL EXTINCTION COEFFICIENT OF P-700 - A BAND SHIFT OF CHLOROPHYLL-A ASSOCIATED WITH OXIDATION OF P-700

    K SONOIKE, S KATOH

    BIOCHIMICA ET BIOPHYSICA ACTA   935 ( 1 ) 61 - 71  1988年08月  [査読有り]

  • ISOLATION OF AN INTRINSIC ANTENNA CHLOROPHYLL A-PROTEIN FROM THE PHOTOSYSTEM-I REACTION CENTER COMPLEX OF THE THERMOPHILIC CYANOBACTERIUM SYNECHOCOCCUS SP

    K SONOIKE, S KATOH

    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS   244 ( 1 ) 254 - 260  1986年01月  [査読有り]

    DOI

▼全件表示

書籍等出版物

  • 光合成

    高橋裕一郎, 園池公毅, 古本強( 担当: 編集,  担当範囲: 編集およびIV.2章の執筆)

    朝倉書店  2021年12月  [査読有り]

  • フォトサイエンス生物図録(新課程)

    島田正和, 酒井建雄, 園池公毅, 田村実, 中野賢太郎, 成川礼, 湯本貴和, 和田洋( 担当: 監修,  担当範囲: 第2編、第6編)

    数研出版  2021年11月 ISBN: 9784410281471

  • ミースフェルド生化学

    Miesfeld, Roger L, McEvoy, Megan M, 稲田, 利文, 水島, 昇, 田口, 英樹, 宮澤, 恵二, 三善, 英知, 村上, 誠, 吉澤, 史昭他( 担当: 共訳,  担当範囲: 第12章「光合成」の翻訳に協力)

    東京化学同人  2020年10月 ISBN: 9784807909865

  • モリス生物学 生命のしくみ

    八杉貞雄, 園池公毅, 和田洋( 担当: 共訳)

    東京化学同人  2020年04月 ISBN: 9784807909612

  • 植物の形には意味がある

    園池 公毅( 担当: 単著)

    ベレ出版  2016年04月 ISBN: 9784860644703

  • 光と生命の事典

    日本光生物学協会編( 担当: 共著,  担当範囲: 「生物による光エネルギーの利用」の項目を執筆)

    朝倉書店  2016年 ISBN: 9784254171617

  • 光合成のエネルギー変換と物質変換

    園池 公毅( 担当: 共著,  担当範囲: 第1章「光合成の全体像」を執筆)

    化学同人  2015年04月 ISBN: 9784759817201

  • 光合成と産業応用最前線

    園池 公毅( 担当: 共著,  担当範囲: 総論「光合成とは」の執筆)

    株式会社NTS  2014年12月 ISBN: 9784860434120

  • 光合成生物の進化と生命科学

    三村徹郎, 川井浩史編( 担当: 共著,  担当範囲: 全8章の内、第1章、第2章を執筆)

    培風館  2014年07月 ISBN: 9784563078133

  • 生物学辞典(第5版)

    園池 公毅( 担当: 分担執筆,  担当範囲: 光合成分野の執筆・編集協力)

    岩波書店  2013年02月 ISBN: 9784000803144

  • トコトンやさしい光合成の本

    園池 公毅( 担当: 単著)

    日刊工業新聞社  2012年12月 ISBN: 9784526069932

  • 生物学辞典

    ( 担当: 共著,  担当範囲: 光合成分野の執筆・編集協力)

    東京化学同人  2010年 ISBN: 9784807907359

  • 光合成とはなにか 生命システムを支える力

    園池公毅( 担当: 単著)

    講談社  2008年 ISBN: 9784062576123

  • Photosystem I : the light-driven plastocyanin : ferredoxin oxidoreductase

    Golbeck, John H., Sonoike, K.( 担当: 共著,  担当範囲: Photoinhibition and Protection of Photosystem I)

    Springer  2006年 ISBN: 1402042558

  • Regulation of photosynthesis

    Aro, Eva-Mari, Andersson, Bertil, Hihara, Yukako, Sonoike, Kintake( 担当: 共著,  担当範囲: Regulation, Inhibition and Protection of Photosystem I)

    Kluwer Academic Publishers  2001年 ISBN: 0792363329

  • Probing photosynthesis : mechanisms, regulation, and adaptation

    Yunus, Mohammad, Pathre, Uday, Mohanty, Prasanna, Ohad, Itzhak, Sonoike, Kintake, Andersson, Bertil( 担当: 共著,  担当範囲: Photoinactivation of the two photosystems in oxygenic photosynthesis-mechanisms and regulations)

    Taylor & Francis  2000年 ISBN: 0748408215

  • Photoinhibition of Photosystem I in Chilling Sensitive Plants Determined in viro and in vitro(共著)

    Photosynthesis : Mechanisms and Effects  1998年

  • Effects of Virus Infection on Photosynthesis of Eupatorium makinoi(共著)

    Photosynthesis : Mechanisms and Effects  1998年

  • The Loss of RuBPCase by 24-hour Treatment of "Rain" in Light(共著)

    Photosynthesis : From Light to Biosphere Kluwer Academic Publishers  1995年

  • Confribution of Lipids to PSII (共著)

    Photosynthesis : From Light to Biosphere Kluwer Academic Publishers  1995年

  • Chilling Sensitive Steps in Leaves of Phaseolus vulgaris L. Examination of the Effects of Growth Irradiances on PSI Photoinhibition(共著)

    Photosynthesis : From Light to Biosphere Kluwer Academic Publishers  1995年

  • Confribution of Lipids to PSII (共著)

    Photosynthesis : From Light to Biosphere Kluwer Academic Publishers  1995年

  • The Function of psaE Gene Product in Reduction of Ferredoxin by Cyanobacterial PSI Reaction Center(共著)

    Kluwer Academic Publishers  1992年

  • Electron Transfer from Cytochrome c-553 to P-700 in Cyanobacterial PSI Reaction Center Complexes with and without the Bound psaF Gene Product(共著)

    Kluwer Academic Publishers  1992年

  • Chemical Environment around the Two Chlorophyll a' Molecules at the Core of Photosystem I(共著)

    Kluwer Academic Publishers  1992年

  • Variations of the Differential Extinction Coefficient of P-700 and Re-estimation of Stoichiometry of Constituents in Photosystem I Reaction Center Complexes from Synechococcus elongatus (共著)

    Kluwer Academic Publishers  1990年

▼全件表示

Misc

  • 光合成の産物がグルコースであるという都市伝説

    園池公毅

    理科教室   789   64 - 69  2020年09月  [招待有り]

    担当区分:筆頭著者, 最終著者, 責任著者

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 光合成を学ぶことによって得られるもの

    園池公毅

    理科教室   786   40 - 45  2020年06月  [招待有り]   [ 国内誌 ]

    担当区分:筆頭著者, 最終著者, 責任著者

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 令和最初の歌会始に寄せて

    園池公毅

    皇室   86   40 - 45  2020年04月  [招待有り]   [ 国内誌 ]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 歌会始改革~より国民へ開かれた行事へ

    園池公毅

    皇室   85   104 - 109  2020年01月  [招待有り]   [ 国内誌 ]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 明治維新と歌会始

    園池公毅

    皇室   84   86 - 91  2019年10月  [招待有り]   [ 国内誌 ]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 和歌の披講とは~その歴史と流れ

    園池公毅

    皇室   83   86 - 91  2019年07月  [招待有り]   [ 国内誌 ]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 大学入学共通テストに向けて

    園池 公毅

    理科通信サイエンスネット   64   1 - 5  2019年05月  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 平成最後の歌会始に寄せて

    園池公毅

    皇室   82   74 - 77  2019年04月  [招待有り]   [ 国内誌 ]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 光合成の飽和曲線

    園池 公毅

    CanAppleニュース   66  2019年02月  [招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 座談会「両陛下のお歌を鑑賞する」

    芳賀徹, 園池公毅, 寺井龍哉, 今野寿美

    短歌研究   76 ( 1 ) 26 - 51  2019年01月

    その他  

  • 初期地球環境の変遷とシアノバクテリア

    園池 公毅

    生物工学   96 ( 11 ) 626 - 629  2018年11月  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 大学入学共通テスト(新テスト)で生物の問題はどう変わるか

    園池 公毅

    理大 科学フォーラム   407   8 - 9  2018年  [招待有り]

    記事・総説・解説・論説等(大学・研究所紀要)  

  • 歌御会始と披講

    園池 公毅

    悠久   154   32 - 45  2018年  [招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 光合成研究の新しい展開

    園池 公毅

    生物の科学 遺伝   72   256 - 259  2018年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 光合成研究へのいざない

    園池 公毅

    ミルシル   42   6 - 9  2014年  [招待有り]

    記事・総説・解説・論説等(大学・研究所紀要)  

  • 光合成における光エネルギーの利用と散逸

    園池 公毅

    光学   43   265 - 271  2014年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 葉緑素の蛍光と光合成

    園池 公毅

    理科教育ニュース   899  2013年  [招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 果実の光合成

    園池 公毅

    光合成研究   22   70 - 76  2012年  [査読有り]  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 光を用いた光合成の解析

    園池 公毅

    未来材料   12   44 - 48  2012年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 最近の光合成研究の進展

    園池 公毅

    太陽エネルギー   37   3 - 9  2011年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 暗記科目でない理科を目指して

    園池 公毅

    中学校教育フォーラム   27   19 - 19  2010年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • ライオンを食べると勇猛になれるか?

    園池 公毅

    早稲田ウィークリー   1226   6 - 6  2010年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 和歌の披講

    園池 公毅

    やすくに   662   6 - 7  2010年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • クロロフィル蛍光と吸収による光合成測定

    園池 公毅

    低温科学   67   507 - 524  2009年  [招待有り]

    記事・総説・解説・論説等(大学・研究所紀要)  

  • 読みやすい文章

    園池 公毅

    読書人の雑誌『本』   387   55 - 57  2008年10月  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 光合成の教科書を振り返る

    園池 公毅

    光合成研究   18   101 - 102  2008年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 熱水生物群集の成り立ち

    園池 公毅

    月刊海洋   40   393 - 398  2008年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 光合成の光環境応答

    園池 公毅

    蛋白質核酸酵素   53   1180 - 1186  2008年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 新しいクロロフィルの研究

    園池 公毅

    中学校教育フォーラム   18   18 - 19  2008年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 光合成の測定

    園池 公毅

    高校理科研究   11   2 - 7  2005年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • パルス変調クロロフィル蛍光測定におけるデータの解釈

    園池 公毅

    日本光合成研究会会報   42   7 - 12  2005年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 損得で決まる寿命

    園池 公毅

    別冊國文学   58   214 - 214  2005年  [招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 植物の寿命-つなぐ命と長寿の秘訣

    園池 公毅

    別冊國文学   58   160 - 163  2005年  [招待有り]

    記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)  

  • 柏キャンパス緑化計画

    園池 公毅

    SOUSEI   4   9 - 9  2004年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 植物の環境ストレス応答

    園池 公毅

    Ajico News   203   1 - 8  2001年  [招待有り]

    記事・総説・解説・論説等(その他)  

  • 科学技術倫理論:科学者のとるべき態度とは?

    園池 公毅

    細胞工学   19   1790 - 1792  2000年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

  • 環境ストレスと光合成

    牧野周, 園池公毅

    化学と生物   32   615 - 618  1994年  [招待有り]

    記事・総説・解説・論説等(学術雑誌)  

▼全件表示

Works(作品等)

  • Fluorome

    園池 公毅  データベース 

    2007年09月
    -
    継続中

     概要を見る

    The Cyanobacterial Chlorophyll Fluorescence Database (Fluorome) is a collection of induction kinetics of chlorophyll fluorescence from cyanobacterial mutant cells of Synechocystis sp. PCC 6803. Chlorophyll fluorescence kinetics, which reflects the modified metabolism in each mutant, was determined for over 750 gene-disruption mutants. One can easily search for mutants that show "similar" phenotype to the mutant of one's own interest.

受賞

  • 特別研究員等審査会専門委員(書面担当)表彰

    2019年07月   日本学術振興会  

    受賞者: 園池 公毅

  • Peer Review Award 2018

    2018年09月   Publons   Top 1% pre-publication peer reviewer in Plant & Animal Science  

    受賞者: 園池 公毅

  • Peer Review Award 2017

    2017年09月   Publons   Top 1% pre-publication peer reviewer in Biochemistry, Genetics and Molecular Biology  

    受賞者: 園池 公毅

  • Excellent Reviewer

    2013年12月   Journal of Photochemistry & Photobiology, B: Biology誌  

    受賞者: 園池 公毅

  • 特別賞

    2013年09月   日本植物学会   光合成に関する啓蒙活動  

    受賞者: 園池 公毅

  • 奨励賞

    1996年09月   日本植物学会   光化学系Ⅰとその低温光阻害のメカニズム  

    受賞者: 園池公毅

▼全件表示

共同研究・競争的資金等の研究課題

  • 新光合成:光エネルギー変換システムの再最適化

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)

    研究期間:

    2016年06月
    -
    2021年03月
     

    皆川 純, 清水 浩, 坂本 亘, 園池 公毅, 宗景 ゆり, 久堀 徹, 魚住 信之, 高橋 裕一郎, 鹿内 利治, 栗栖 源嗣, 矢守 航

     概要を見る

    光合成センターの運営 ― 光合成機能解析センター、光合成リソースセンターは、当初予定通り運営されている。
    領域研究の活性化 ― 毎月の月初めのSkypeによる総括班会議において、さまざまな領域運営の議論を行い、領域活動の支援と活性化を行った。また、全計画班、全公募班による年2回の領域会議を行い、領域活動の活発化を進め、多数の共同研究を推進した。領域の主催、共催による、シンポジウム、技術講習会等を多数開催した。
    広報活動 ― ニュースレターを2回発刊した。
    中間評価とりまとめ ― 本年度は中間評価を行う3年目にあたっていたため、過去2年間の領域活動のとりまとめ作業を、総括班が中心となって行った。

  • プロトン勾配による集光のフィードバック制御

    日本学術振興会  科学研究費助成事業 新学術領域研究(研究領域提案型)

    研究期間:

    2016年06月
    -
    2021年03月
     

    皆川 純, 秋本 誠志, 園池 公毅

     概要を見る

    1.NPQ作動原理の解明 II ― 強光を当てた時にLHCSR3タンパク質が合成されqEクエンチングを引き起こすことは知られていたが、よく似たLHCSR1については、その存在こそ知られていたものの、よくわかっていなかった。そこで、通常の人工照明ではなく紫外線を含む照明でクラミドモナスを培養し、LHCSR1を蓄積させた状態でピコ秒領域での蛍光減衰過程の詳細な解析を行い、qEクエンチングが光化学系IIから光化学系Iへのエネルギー移動、および光化学系Iにおける光化学反応にて起こることを明らかにした。
    2.強光シグナル伝達系の解明II ― 強光シグナル伝達系が概日リズムの影響を受けることは知られていたがその分子機構は明らかではない。そこで概日リズム変異株を解析し、強光シグナルと概日リズムの関連を明らかにした。これまでに単離された105のroc(rhythm of chloroplast)変異株の中から赤色光環境下で特に高いqEクエンチング能を持つroc75変異株に注目した。この赤色光環境下でROC75を欠失するとLHCSR3発現レベルが高かったことからROC75は赤色によって活性化されるLHCSR3の負の制御因子であることがわかった。また、青色光環境下ではクリプトクロムを欠損(pcry)するとROC75発現量が下がったことから、pCRYがROC75を正に制御することも明らかとなった。以上よりROC75は青色光受容体であるpCRYによって発現量が正に制御され、赤色光によって活性化される、LHCSR3の負の制御因子であることが判明した。

  • 果実の光合成装置の特殊性

    日本学術振興会  科学研究費補助金挑戦的萌芽研究

    研究期間:

    2016年04月
    -
    2019年03月
     

    園池 公毅

  • クロロフィル蛍光による細胞内代謝推定システムの構築

    日本学術振興会  科学研究費補助金基盤研究(B)

    研究期間:

    2016年04月
    -
    2019年03月
     

    園池 公毅

  • クロロフィル蛍光を用いた新規薬剤スクリーニング法の開発

    日本学術振興会  科学研究費補助金挑戦的萌芽研究

    研究期間:

    2011年04月
    -
    2014年03月
     

    園池 公毅

  • 時系列蛍光データを用いた表現型データベースの構築

    日本学術振興会  科学研究費補助金特定研究

    研究期間:

    2006年04月
    -
    2008年03月
     

    園池 公毅

  • 時系列蛍光データを用いた表現型データベースの構築

    日本学術振興会  科学研究費補助金特定研究

    研究期間:

    2005年04月
    -
    2006年03月
     

    園池 公毅

  • シアノバクテリア遺伝子のゲノムワイドな機能クラスタリング

    日本学術振興会  科学研究費補助金特定研究

    研究期間:

    2004年04月
    -
    2005年03月
     

    園池 公毅

  • 2次元蛍光スクリーニングによる光合成制御因子の解析

    日本学術振興会  科学研究費補助金基盤研究(B)

    研究期間:

    2002年04月
    -
    2005年03月
     

    園池 公毅

  • シアノバクテリア遺伝子のゲノムワイドな機能クラスタリング

    日本学術振興会  科学研究費補助金特定研究

    研究期間:

    2003年04月
    -
    2004年03月
     

    園池 公毅

  • シアノバクテリアがコードする遺伝子産物機能の網羅的解析

    日本学術振興会  科学研究費補助金特定領域研究(C)

    研究期間:

    2001年04月
    -
    2002年03月
     

    園池 公毅

  • 光阻害の観点から見た植物の低温障害の研究

    日本学術振興会  科学研究費補助金基盤研究(B)

    研究期間:

    1999年04月
    -
    2002年03月
     

    園池 公毅

  • シアノバクテリアゲノムがコードする遺伝子産物機能の網羅的解析

    日本学術振興会  科学研究費補助金特定領域研究(C)

    研究期間:

    2000年04月
    -
    2001年03月
     

    園池 公毅

  • 光合成の光阻害における光化学系I反応中心の分解メカニズム

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1997年04月
    -
    1999年03月
     

    園池 公毅

  • Molecular analysis of components that regulate photosynthetic energy conversion

    Human Frontier Science Program  Research Grant

    研究期間:

    1996年
    -
    1998年
     

    R. Herrmann

  • 光合成の光阻害反応における光化学系Iと光化学系IIの相互調節

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1996年04月
    -
    1997年03月
     

    園池 公毅

  • 植生の回復を目的とする耐ストレス植物の作出

    地球環境産業技術研究機構  優秀研究企画(受託研究)

    研究期間:

    1995年04月
    -
    1997年03月
     

    園池 公毅

  • 光化学系Iの光阻害における低温と酸素の役割

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1995年04月
    -
    1996年03月
     

    園池 公毅

  • 系I反応中心複合合体の小型サブユニットPSI-Lの機能と表在性サブユニットとの関係

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1994年04月
    -
    1995年03月
     

    園池 公毅

  • 光化学系I反応中心複合体の小型サブユニットPSI-Eの役割

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1993年04月
    -
    1994年03月
     

    園池 公毅

  • 光化学系I反応中心複合体の小型サブユニット(psaI遺伝子産物)の役割

    日本学術振興会  科学研究費補助金奨励研究(A)

    研究期間:

    1992年04月
    -
    1993年03月
     

    園池 公毅

  • Environmental Response of Photosynthesis

    Ordinary Research

▼全件表示

講演・口頭発表等

  • シアノバクテリアのアミノ酸添加による細胞内レドックスの変化

    伊藤佑真, 園池公毅

    日本植物生理学会第63回年会  

    発表年月: 2022年03月

    開催年月:
    2022年03月
     
     
  • Interaction of redox conditions between PQ and NADP in Synechocystis sp. PCC 6803

    Takako Ogawa, Kintake Sonoike  [招待有り]

    Japan-German Binational Seminar 2022  

    発表年月: 2022年03月

    開催年月:
    2022年03月
     
     
  • 植物の葉の形や色と光合成

    園池公毅  [招待有り]

    東京都立成瀬高等学校講演会  

    発表年月: 2021年09月

  • Metabolic interaction in cyanobacteria

    Ogawa,T, Suzuki,K, Sonoike,K  [招待有り]

    Japan-Finland Seminar 2021  

    発表年月: 2021年08月

    開催年月:
    2021年08月
     
     
  • シアノバクテリアSynechocystis sp. PCC6803におけるプラストキノンプールのレドックスと非光化学消光の関係性

    太刀掛稜平, 園池公毅

    日本植物生理学会第62回年会  

    発表年月: 2021年03月

    開催年月:
    2021年03月
     
     
  • シアノバクテリアにおいて時計制御因子RpaAが光合成電子伝達制御を介して増殖至適温度を制御する

    長谷川葉月, 鶴巻達大, 今村壮輔, 園池公毅, 田中寛

    日本植物生理学会第62回年会  

    発表年月: 2021年03月

    開催年月:
    2021年03月
     
     
  • 大学入学共通テストで理科・生物はどう変わるか?

    園池 公毅  [招待有り]

    東京理科大学理数教育研究センター研究会   (東京理科大学神楽坂キャンパス)  東京理科大学教育支援機構理数教育研究センター  

    発表年月: 2019年12月

  • 大学入学共通テストに向けて -理科・生物の場合-

    園池 公毅  [招待有り]

    数研セミナー   (数研出版東京本社(東京))  数研出版株式会社  

    発表年月: 2019年11月

     概要を見る

    主に関東の高校教員を対象に大学入試制度改革の現状と展望を解説する

  • 大学入学共通テストに向けて -理科・生物の場合-

    園池 公毅  [招待有り]

    数研セミナー   (数研出版関西本社(京都))  数研出版株式会社  

    発表年月: 2019年11月

     概要を見る

    主に関西の高校教員を対象に大学入試制度改革の現状と展望を解説する

  • 大学入試の変革について

    園池公毅  [招待有り]

    新学習指導要領実施に向けたシンポジウム2019   (新渡戸文化学園東高円寺キャンパス)  東京都生物教育研究会・日本生物教育会  

    発表年月: 2019年10月

  • 生物の大学入試はどう変わるか?

    園池公毅  [招待有り]

    生科連公開シンポジウム2019   (東京)  生物科学学会連合  

    発表年月: 2019年10月

  • Synechocystis sp. PCC 6803のslr0400破壊株は従属栄養性を獲得する

    石川優真, 宮城敦子, 長野稔, 石川寿樹, 山口雅利, 園池公毅, 金子康子, 日原由香子, 川合真紀

    日本植物学会第83回大会  

    発表年月: 2019年09月

  • Screening of mutants using chlorophyll fluorescence

    園池公毅  [招待有り]

    日本植物学会第83回大会   (宮城県仙台市)  日本植物学会  

    発表年月: 2019年09月

  • 光合成よもやま話

    園池公毅  [招待有り]

    東京都生物教育研究会記念講演会   (筑波大学付属駒場中・高等学校創立50周年記念会館) 

    発表年月: 2019年07月

  • 単細胞紅藻Galdieira sulphurariaにおける高濃度CO2が増殖および光合成に与える影響

    尾関大徳, 三角将洋, 園池公毅, 伊藤恵美, 蓑田歩

    日本光合成学会第10回年会およびシンポジウム   (京都産業大学むすびわざ館)  日本光合成学会  

    発表年月: 2019年05月

  • シアノバクテリアの窒素飢餓からの回復におけるATP合成機構

    新森友香, 園池公毅, 得平茂樹

    日本光合成学会第10回年会およびシンポジウム   (京都産業大学むすびわざ館)  日本光合成学会  

    発表年月: 2019年05月

  • Synechocystis sp. PCC 6803における細胞内イソクエン酸量の増加と光化学系量比調節の関係

    太刀掛稜平, 園池公毅

    日本光合成学会第10回年会およびシンポジウム   (京都産業大学むすびわざ館)  日本光合成学会  

    発表年月: 2019年05月

  • Euglena gracilisの青色光依存的なカロテノイド合成と光順化に及ぼす影響

    丹野夕麗, 加藤翔太, 高橋宜治, 園池公毅, 児玉豊, 高市真一, 篠村知子

    日本植物生理学会第60回年会   (名古屋大学東山キャンパス)  日本植物生理学会  

    発表年月: 2019年03月

  • シアノバクテリアSynechocystis sp. PCC6803が有するNADキナーゼの生理的役割

    石川優真, 宮城敦子, 石川寿樹, 長野稔, 山口雅利, 園池公毅, 日原由香子, 金子康子, 川合真紀

    日本植物生理学会第60回年会   (名古屋大学東山キャンパス)  植物生理学会  

    発表年月: 2019年03月

  • 切葉で見られる光化学系Ⅰの特異的阻害

    佐藤優紀, 園池公毅

    日本植物生理学会第60回年会   (名古屋大学東山キャンパス)  日本植物生理学会  

    発表年月: 2019年03月

  • 暗所でも見られるクラミドモナスの非光化学消光は有機炭素源の有無よりも培養光強度により影響を受ける

    三角将洋, 園池公毅

    日本植物生理学会第60回年会   (名古屋大学東山キャンパス)  日本植物生理学会  

    発表年月: 2019年03月

  • 莢の透過率はマメの種皮の光合成に影響を与えるのか

    田代周与, 園池公毅

    日本植物生理学会第60回年会   (名古屋大学東山キャンパス)  日本植物生理学会  

    発表年月: 2019年03月

  • 和歌を歌う~歌会始

    園池公毅  [招待有り]

    声明関連講座「日本の声をめぐって」   (愛知芸術文化センター)  愛知県芸術劇場  

    発表年月: 2019年02月

     概要を見る

    宮中の年中行事の一つである歌会始では、一般からの詠進歌から選ばれた歌や皇族方のお歌などが、天皇皇后両陛下の御前で披講されます。声に出して読み上げることにより和歌を鑑賞する披講は、千年以上の歴史を持ち、現代の歌会始に受け継がれています。本講演では、そのような和歌の披講の歴史と技法について実演を交えながらご紹介したいと思います。

  • 「歌会始」のこと「和歌披講」のこと

    園池公毅  [招待有り]

    源氏物語ひとくち講座   江戸川区立中央図書館  

    発表年月: 2018年11月

  • 青色光照射の及ぼすEuglena gracilisのカロテノイド組成や光合成活性への影響

    丹野夕麗, 加藤翔太, 高橋宣治, 高市真, 園池公毅, 児玉豊, 篠村知子

    第34回ユーグレナ研究会   (沖縄県石垣島) 

    発表年月: 2018年11月

  • クロロフィル蛍光による代謝解析

    園池 公毅  [招待有り]

    日本フィンランド二国間セミナー2018   (神戸) 

    発表年月: 2018年09月

  • 生物教育の未来-入試・教科書・検定の三すくみからの脱出を目指して-

    園池 公毅  [招待有り]

    日本植物学会内82回大会  

    発表年月: 2018年09月

  • 単細胞性紅藻Galdieria sulphurariaにおける高濃度CO2の光合成特性への影響

    尾関大徳, 三角将洋, 園池公毅, 蓑田歩

    日本植物学会第82回大会  

    発表年月: 2018年09月

  • 単細胞性紅藻Galdieria sulphurariaの高濃度CO2の増殖への影響

    尾関大徳, 園池公毅, 蓑田歩

    日本光合成学会第9回年会およびシンポジウム   (仙台) 

    発表年月: 2018年05月

  • 切葉で見られる光化学系Iの特異的阻害

    佐藤優紀, 園池公毅

    日本光合成学会第9回年会およびシンポジウム   (仙台)  日本光合成学会  

    発表年月: 2018年05月

  • 水に溶かした色素タンパク質や膜標品のHPLC直接導入法による色素分析方法の検討

    高市真一, 大越慧, 大友征宇, 三角将洋, 園池公毅

    日本植物生理学会題59回年会   (札幌) 

    発表年月: 2018年03月

  • 光合成から見たボルボックスの生活環と細胞分化

    吉鴻一, 関根恒平, 寺島一郎, 園池公毅

    日本植物生理学会題59回年会   (札幌) 

    発表年月: 2018年03月

  • マメ科植物の種子の光合成の特殊性

    杉本和弥, 園池公毅

    日本植物生理学会題59回年会   (札幌) 

    発表年月: 2018年03月

  • シアノバクテリアSynechocystis sp. PCC6803におけるNADキナーゼの異なる役割

    石川優真, 宮城敦子, 石川寿樹, 長野稔, 山口雅利, 園池公毅, 日原由香子, 金子康子, 河合真紀

    日本植物生理学会題59回年会   (札幌) 

    発表年月: 2018年03月

  • クワ腋芽伸長枝の葉序にみられる二列互生−螺旋モード転換のメカニズムの解析

    加藤壮一郎, 園池公毅

    日本植物生理学会題59回年会   (札幌) 

    発表年月: 2018年03月

  • シアノバクテリアSynechocystis sp. PCC 6803の緊縮応答関連遺伝子 (slr1325,sll1546)の変異は細胞内のレドックスを酸化的にする

    三瓶雅俊, 園池公毅

    藍藻の分子生物学 2017  

    発表年月: 2017年12月

  • シアノバクテリアは 宇宙環境を生き延びられるか

    園池公毅, 木村駿太, 加藤浩, 安部智子, 大森正之, 富田-横谷香織, たんぽぽWG  [招待有り]

    藍藻の分子生物学 2017  

    発表年月: 2017年12月

  • 色素タンパク複合体や膜試料の直接導入によるHPLC色素分析方法の検討

    高市真一, 大友征宇, 園池公毅

    第31回カロテノイド研究談話会   (京都) 

    発表年月: 2017年09月

  • シアノバクテリアSynechocystis sp. PCC 6803における代謝系間相互作用を利用したクロロフィル蛍光による遺伝子機能解析

    小川敬子, 鈴木健太, 園池公毅

    日本植物生理学会第58回年会   (鹿児島) 

    発表年月: 2017年03月

  • 陸棲藍藻 Nostoc sp. HK-01 乾燥藻体の宇宙環境耐性(P-113)

    富田‐横谷香織, 木村駿太, 井上琴美, 加藤浩, 安部智子, 園池公毅, 大森正之, たんぽぽWG

    第17回宇宙科学シンポジウム   (相模原) 

    発表年月: 2017年01月

  • 光阻害から系Iを保護する機構のプロトプラストを用いた局在解析

    竹内彩絵, 園池公毅

    日本植物学会第80回大会   (沖縄) 

    発表年月: 2016年09月

  • 灰色藻Cyanophora paradoxaと紅藻Cyanidioschyzon merolaeの葉緑体呼吸と光順応の比較

    三角将洋, 園池公毅

    日本植物学会第80回大会   (沖縄) 

    発表年月: 2016年09月

  • シアノバクテリアにおける翻訳関連遺伝子の破壊の影響は光化学系系?還元側を通してクロロフィル蛍光に反映される

    小川敬子, 鈴木健太, 園池公毅

    日本植物学会第80回大会   (沖縄) 

    発表年月: 2016年09月

  • Respiration affects photosynthesis through the reducing side of photosystem I in cyanobacterium Synechocystis sp. PCC 6803

    Ogawa, T, Suzuki, K, Sonoike, K  [招待有り]

    Finnish-Japanese symposium 2016   (Saariselka,Finland) 

    発表年月: 2016年09月

  • Probing cyanobacterial redox and energy distribution by chlorophyll fluorescence

    Misumi, M, Ogawa, T, Katoh, H, Tomo, T, Sonoike, K  [招待有り]

    Finnish-Japanese symposium 2016   (Saariselka,Finland) 

    発表年月: 2016年09月

  • The chlororespiration and photoprotection in Glaucophyta Cyanophora paradoxa

    Misumi, M, Sonoike, K

    17th International Congress on Photosynthesis Research: Photosynthesis in a Changing World   (Maastricht,The Netherlands) 

    発表年月: 2016年08月

  • Estimation of photosynthesis by chlorophyll fluorescence measurements of cyanobacteria and eukaryotic algae

    Ogawa, T, Misumi, M, Sonoike, K

    17th International Congress on Photosynthesis Research: Photosynthesis in a Changing World   (Maastricht,The Netherlands) 

    発表年月: 2016年08月

  • Variation of redox state of plastoquinone pool in cyanobacteria revealed by photochemical and non-photochemical quenching of chlorophyll fluorescence

    Misumi, M, Ogawa, T, Katoh, H, Tomo, T, Sonoike, K  [招待有り]

    International Conference Photosynthesis Research for Sustainability   (Pushchino,Russia) 

    発表年月: 2016年06月

  • シアノバクテリアと藻類における呼吸と光合成の相互作用

    園池公毅, 三角将洋, 鈴木健太, 小川敬子, 加藤浩, 鞆達也

    日本植物生理学会第57回年会   (岩手) 

    発表年月: 2016年03月

  • シアノバクテリアSynechocystis sp. PCC 6803における翻訳関連遺伝子の破壊がクロロフィル蛍光に与える影響

    小川敬子, 鈴木健太, 園池公毅

    日本植物生理学会第57回年会   (岩手) 

    発表年月: 2016年03月

  • Growth light conditions and mechanisms that make Alocasia odora resistant to PSI photoinhibition induced by fluctuating light

    松尾光敏, 河野優, 園池公毅, 寺島一郎

    日本植物生理学会第57回年会   (岩手) 

    発表年月: 2016年03月

  • 陸棲藍藻 Nostoc sp. HK-01 乾燥藻体の高宇宙環境耐性

    富田-横谷香織, 木村駿太, 木村靖子, 井上琴美, 味岡令子, 佐藤誠吾, 加藤浩, 安部智子, 園池公毅, 大森正之, たんぽぽWG

    第16回宇宙科学シンポジウム   (相模原) 

    発表年月: 2016年01月

  • クロロフィル蛍光測定が示すシアノバクテリアの多様性と普遍性

    三角将洋, 加藤浩, 鞆達也, 園池公毅

    藍藻の分子生物学 2015  

    発表年月: 2015年11月

  • フィコビリン量によらずシアノバクテリアの光化学系Ⅱ量子収率をクロロフィル蛍光により見積もる方法

    小川敬子, 園池公毅

    藍藻の分子生物学 2015  

    発表年月: 2015年11月

  • シアノバクテリアにおいて呼吸基質が光合成電子伝達に与える経路の解明

    鈴木健太, 青木彩夏, 小川敬子, 園池公毅

    日本植物学会第79回大会   (新潟) 

    発表年月: 2015年09月

  • Problems and its exploitation of chlorophyll fluorescence measurement in cyanobacterium Synechocystis sp. PCC 6803

    Ogawa, T, Sonoike K

    15th International Symposium on Phototrophic Prokaryotes   (Tubingen,Germany) 

    発表年月: 2015年08月

  • Cyanobacterial diversity and uniformity froma chlorophyll fluorescence point of view

    Misumi, M, Sonoike K

    15th International Symposium on Phototrophic Prokaryotes   (Tubingen,Germany) 

    発表年月: 2015年08月

  • 窒素欠乏条件下におけるシアノバクテリアの光合成量子収率の新しい評価法による解析

    小川敬子, 園池公毅

    第6回日本光合成学会年会および公開シンポジウム   (岡山) 

    発表年月: 2015年05月

  • シアノバクテリア,緑藻,紅藻で比較した呼吸が光合成に与える影響

    三角将洋, 加藤浩, 鞆達也, 園池公毅

    第6回日本光合成学会年会および公開シンポジウム   (岡山) 

    発表年月: 2015年05月

  • Effects of metabolic modification on the redox state of photosynthetic electron transfer in cyanobacteria

    Ogawa, T, Suzuki, K, Aoki, A, Misumi, M, Sonoike, K

    The German-Japanese Binational Seminar 2015 "Harvesting Light: From light to biotechnological products"   (Atami,Japan) 

    発表年月: 2015年03月

  • 呼吸および葉緑体呼吸がプラストキノンプールの酸化還元状態に与える影響から見た藻類の多様性

    三角将洋, 鞆達也, 園池公毅

    日本植物生理学会第56回年会   (東京) 

    発表年月: 2015年03月

  • クロロフィル蛍光測定から見えるシアノバクテリアの代謝系相互作用

    小川敬子, 鈴木健太, 園池公毅

    日本植物生理学会第56回年会   (東京) 

    発表年月: 2015年03月

  • 陸棲ラン藻 Nostoc sp. HK-01 乾燥藻体の高宇宙環境耐性

    富田-横谷香織, 木村駿太, 木村靖子, 味岡令子, 佐藤誠吾, 加藤浩, 安部智子, 園池公毅, 大森正之, たんぽぽWG

    第15回宇宙科学シンポジウム   (相模原) 

    発表年月: 2015年01月

  • 光合成解析の実例:シアノバクテリア・藻類の解析

    園池公毅

    PAMを用いた光合成解析技術セミナー   (東京) 

    発表年月: 2014年11月

  • Probing Atypical Photosynthesis

    Sonoike, K, Kitahara, S, Ueno, T, Tamai, J  [招待有り]

    Japanese-Finnish Seminar 2014 "Design of Superior Machinery of Light Energy Conversion in Photosynthetic Organisms"   (Jozankei,Hokkaido,Japan) 

    発表年月: 2014年10月

  • 葉とカルスの光合成の比較?シロイヌナズナのクロロフィル蛍光解析から?

    北原新也, 園池公毅

    日本植物学会第78回大会   (川崎) 

    発表年月: 2014年09月

  • ソラマメの種子の光合成の特殊性

    上野朋裕, 玉井絢子, 園池公毅

    日本植物学会第78回大会   (川崎) 

    発表年月: 2014年09月

  • シアノバクテリアの呼吸鎖におけるプラストキノンへの電子供給経路の解析

    鈴木健太, 小川敬子, 園池公毅

    日本植物学会第78回大会   (川崎) 

    発表年月: 2014年09月

  • Fe deficiency induces phosphorylation and translocation of the light-harvesting antenna Lhcb1 in thylakoid membranes of barley

    Saito, A, Shimizu, M, Nakamura, H, Maeno, S, Katase, R, Ogawa, T, Miwa, E, Higuchi, K, Sonoike, K

    XVII International Symposium on Iron Nutrition and Interactions in Plants   (Gatersleben,Germany) 

    発表年月: 2014年06月

  • シアノバクテリアのNPQの光強度依存性を決める要因の解析

    小川敬子, 園池公毅

    第5回日本光合成学会年会および公開シンポジウム   (奈良) 

    発表年月: 2014年05月

  • シアノバクテリアsll0381遺伝子の破壊は電子伝達下流の阻害を引き起こす

    立川有佳, 園池公毅

    第5回日本光合成学会年会および公開シンポジウム   (奈良) 

    発表年月: 2014年05月

  • シアノバクテリアの光阻害防御機構における熱放散の役割

    草間友里, 井上修平, 園池公毅, 高市真一, 西山佳孝

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • 能動過程としてのシアノバクテリアの大規模転写抑制機構

    高野壮太朗, 園池公毅, 岩崎秀雄

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • 光化学系?における脂質の役割−クラミドモナスSQDG欠損株1のSQDGの添加効果

    蓑田歩, 佐藤典裕, 園池公毅, 都築幹夫

    日本植物学会第62回大会   (東広島) 

    発表年月: 2014年03月

  • シアノバクテリアSynechocystis sp. PCC 6803の代謝に薬剤添加が及ぼす影響の解析

    青木彩夏, 園池公毅

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • クロロフィル蛍光測定によるシアノバクテリアの呼吸およびCO2取込み能の解析

    小川敬子, 園池公毅

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • アイスプラントのCAM化過程のクロロフィル蛍光測定による解析

    松岡達也, 是枝晋, 園池公毅

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • シアノバクテリアのクロロフィル蛍光測定

    園池公毅

    「生体機能物質の機能および制御の解析とバイオデバイスへの応用」第1回ワークショップ   (横浜) 

    発表年月: 2014年03月

  • 光化学系?を光阻害から防御する機構の局在解析

    矢野健治郎, 田中赳裕, 園池公毅

    日本植物生理学会第55回年会   (富山) 

    発表年月: 2014年03月

  • NDH複合体の変異が光合成に与える影響

    小川敬子, 園池公毅

    ラン藻の分子生物学2013   (木更津) 

    発表年月: 2013年11月

  • シアノバクテリア光化学系IIの光防御機構におけるカロテノイドの役割

    草間友里, 井上修平, 園池公毅, 高市真一, 西山佳孝

    第4回日本光合成学会年会およびシンポジウム   (名古屋) 

    発表年月: 2013年05月

  • シアノバクテリアのndhF1遺伝子の破壊は光合成速度を見かけ上高くする

    小川敬子, 園池公毅

    第4回日本光合成学会年会およびシンポジウム   (名古屋) 

    発表年月: 2013年05月

  • シアノバクテリアの暗期での大規模な転写抑制は光合成の停止によって引き起こされるのか(優秀発表賞受賞)

    高野荘太朗, 園池公毅, 岩崎秀雄

    第7回日本ゲノム微生物学会年会   (長浜) 

    発表年月: 2013年03月

  • 光合成反応で計る植物のストレス

    園池公毅

    津波被災農地におけるストレスの計測と軽減に関するセミナー   (岩手) 

    発表年月: 2012年09月

  • シアノバクテリアのNADH脱水素酵素複合体がプラストキノンプールの酸化還元状態に与える影響

    小川敬子, 小川晃男, 池内昌彦, 原田哲行, 園池公毅

    日本植物学会第76回大会   (姫路) 

    発表年月: 2012年09月

  • Effect of cyanobacterial NDH complex on the redox state of plastoquinone pool

    Ogawa, T, Ogawa, T, Ikeuchi, M, Sonoike, K  [招待有り]

    Japanese-Finnish Seminar 2012 "Photosynthetic Research for Sustainable Energy Production"   (Naantali,Finland) 

    発表年月: 2012年09月

  • 光合成の光エネルギー変換効率は改善可能か?

    園池公毅

    日本植物生理学会第53回年会   (京都) 

    発表年月: 2012年03月

  • Probing metabolic interactions in cyanobacterial cells by chlorophyll fluorescence measurements

    Sonoike, K, Ogawa, T, Harada, T, Ozaki, H  [招待有り]

    Binational Seminar Germany-Japan "Microalgal Products: From Metabolic Fundamentals to Promising Applications"   (Freiburg,Germany) 

    発表年月: 2011年10月

  • シアノバクテリアの呼吸によるプラストキノンプールの還元

    園池公毅

    ラン藻ゲノム研究交流会   (東京) 

    発表年月: 2011年07月

  • 最近の光合成研究の進展

    園池公毅

    日本太陽エネルギー学会太陽光化学部会第3回研究講演会「人工光合成」   (東京) 

    発表年月: 2011年06月

  • ソラマメの豆の光合成(優秀発表賞受賞)

    玉井絢子, 園池公毅

    第2回日本光合成学会年会およびシンポジウム   (京都) 

    発表年月: 2011年06月

  • 光合成とは何か

    園池公毅

    第58回応用物理関係連合講演会   (厚木) 

    発表年月: 2011年03月

  • シアノバクテリアNADH脱水素酵素複合体の活性はプラストキノンプールの還元を通じて光合成のステート遷移に影響を与える

    原田哲行, 尾崎洋史, 園池公毅

    日本植物生理学会第52回年会   (仙台) 

    発表年月: 2011年03月

  • Responses of photosynthetic machinery to iron deficiency in barley

    Saito, K, Higuchi, K, Sonoike, K  [招待有り]

    Japanese-Finnish Seminar 2010 "Future prospects of photosynthetic organisms: from genomes to environment"   (Okayama,Japan) 

    発表年月: 2011年03月

  • 鉄欠乏オオムギ葉のチラコイド膜における光化学系 II 集光性クロロフィル結合タンパク質の挙動解析

    齋藤彰宏, 樋口恭子, 園池公毅

    日本植物生理学会第51回年会   (熊本) 

    発表年月: 2010年03月

  • シアノバクテリアの強光応答

    園池公毅

    東京農業大学先端研究シンポジウム「植物の力を引き出す2」   (東京) 

    発表年月: 2010年03月

  • 鉄欠乏環境下のオオムギにおける光合成装置のリモデリングに関わる分子機構

    齋藤彰宏, 樋口恭子, 園池公毅

    日本植物学会第73回大会   (山形) 

    発表年月: 2009年09月

  • 光合成と地球環境問題

    園池公毅

    日本植物学会第73回大会   (山形) 

    発表年月: 2009年09月

  • 鉄欠乏オオムギ葉緑体におけるLhcb1蛋白質に依存した光保護機構の解析

    齋藤彰宏, 園池公毅, 三輪睿太郎, 樋口恭子

    日本植物生理学会第50回年会   (名古屋) 

    発表年月: 2009年03月

  • 葉緑体チラコイド膜の可逆的構造変化と葉の進展速度およびスターチ合成との関係

    野末はつみ, 馬屋原一平, 亀谷清和, 園池公毅, 林田信明, 野末雅之

    日本植物生理学会第50回年会   (名古屋) 

    発表年月: 2009年03月

  • 変異株のクロロフィル蛍光から機能を推定できる遺伝子群とその特徴

    尾崎洋史, 園池公毅

    日本植物生理学会第50回年会   (名古屋) 

    発表年月: 2009年03月

  • Gene Function and Chlorophyll Fluorescence: Knocking the Cell

    Ozaki, H, Sonoike, K  [招待有り]

    Japanese-Finnish Seminar 2008 "Genomics and Molecular Mechanisms of Regulation in Photosynthetic Organisms"   (Vuoranta,Helsinki,Finland) 

    発表年月: 2008年10月

  • クロロフィル蛍光イメージングを利用したゲノムワイドな遺伝子機能の可能性を探る

    尾崎洋史, 園池公毅

    日本植物学会第72回大会   (高知) 

    発表年月: 2008年09月

  • 弱光順化させると光化学系I/光化学系IIが低いシアノバクテリアslr0249変異株の解析

    尾崎洋史, 園池公毅

    日本植物学会第72回大会   (高知) 

    発表年月: 2008年09月

  • 遺伝子変異と薬剤がクロロフィル蛍光におよぼす変化の定量的解析

    園池公毅

    ラン藻ゲノム研究交流会   (東京) 

    発表年月: 2008年07月

  • 阻害剤と遺伝子破壊がシアノバクテリアのクロロフィル蛍光強度の経時変化にもたらす影響と遺伝子機能予測

    尾崎洋史, 園池公毅

    日本植物生理学会第49回年会   (札幌) 

    発表年月: 2008年03月

  • パルス変調蛍光測定法の基礎講座:原理と測定法

    園池公毅

    日本植物生理学会シンポジウム:クロロフィル蛍光でわかる光合成機能   (札幌) 

    発表年月: 2008年03月

  • シロイヌナズナ変異体cfa1は酸素依存的な光合成系路に欠損を示す

    花野井和弘, 黒澤真理, 園池公毅

    日本植物生理学会第49回年会   (札幌) 

    発表年月: 2008年03月

  • 光合成と環境

    園池公毅

    第34回バイオーム研究会   (柏) 

    発表年月: 2008年01月

  • 光合成におけるエネルギー伝達・電子伝達の調節

    園池公毅

    BMB2007   (横浜) 

    発表年月: 2007年12月

  • Knock on cells to reveal the function of genes: A case study of genes involving in the regulation of photosystem stoichiometry under high light

    Kintake Sonoike  [招待有り]

    US-Japan seminar on "Phenotypic plasticity in response to environmental change: Scaling from the molecular to ecosystem level"   (Nikko,Japan) 

    発表年月: 2007年10月

  • 光エネルギー供給のバランス維持は生態系で役に立つか

    園池公毅

    日本植物学会第71回大会   (野田) 

    発表年月: 2007年09月

  • 遺伝子破壊がシアノバクテリアのクロロフィル蛍光挙動におよぼす影響の定量的解析

    尾崎 洋史, 園池 公毅

    日本植物学会第71回大会   (野田) 

    発表年月: 2007年09月

  • 遺伝子破壊がSynechocystis sp.のクロロフィル蛍光挙動におよぼす影響の定量的解析

    尾崎洋史, 園池公毅

    ラン藻ゲノム研究交流会   (東京) 

    発表年月: 2007年07月

  • Genome-wide analysis of gene functions by chlorophyll fluorescence

    Ozaki, H, Sonoike, K

    14th International Congress of Photosynthesis   (Glasgow, Scotland) 

    発表年月: 2007年07月

  • Sll1961, a regulator of photosystem stoichiometry, is also involved in a novel phycobilisome degradation pathway during nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803

    Sato, H, Pakrasi, H, Sonoike, K

    9th Cyanobacterial Molecular Biology Workshop   (WI,USA) 

    発表年月: 2007年06月

  • 葉緑体局在型NADKによる炭素および窒素代謝の制御

    高橋秀行, 橋田慎之介, 田中歩, 園池公毅, 川合真紀, 内宮博文

    日本植物生理学会2007年度年会   (松山) 

    発表年月: 2007年03月

  • 成熟葉緑体チラコイド膜の構造変化とこれに伴う葉緑体の機能変化

    野末はつみ, 鈴木健二, 渋谷奈々恵, 金子康子, 園池公毅, 林田信明

    日本植物生理学会2007年度年会   (松山) 

    発表年月: 2007年03月

  • 植物葉緑体SIG1の光合成装置PSI遺伝子の転写調節における機能的役割

    戸澤譲, 寺石政義, 佐々木忠将, 園池公毅

    日本植物生理学会2007年度年会   (松山) 

    発表年月: 2007年03月

  • クロロフィル合成変異株におけるグラナ構造の解析

    中西弘充, 野末はつみ, 金子康子, 園池公毅, 田口悟朗, 林田信明

    日本植物生理学会2007年度年会   (松山) 

    発表年月: 2007年03月

  • Synechocystis sp. PCC 6803の光化学系量比調節に関わる因子Sll1961による転写制御

    田森美緒, 藤森玉輝, 尾崎洋史, 佐藤華代, 日原由香子, 園池公毅

    日本植物生理学会2007年度年会   (松山) 

    発表年月: 2007年03月

  • 複数のシアノバクテリアにおけるステート遷移の比較

    島田和美, 藤森玉輝, 園池公毅

    日本植物学会第70回大会   (熊本) 

    発表年月: 2006年09月

  • 強光順化させたシロイヌナズナFtsH6変異体の解析

    臼杵裕之, 園池公毅

    日本植物学会第70回大会   (熊本) 

    発表年月: 2006年09月

  • トマト(Micro-Tom)の低温処理による光合成障害の解析

    高橋万有, 園池公毅

    日本植物学会第70回大会   (熊本) 

    発表年月: 2006年09月

  • ゲノムから見た光合成のステート遷移

    園池公毅

    日本植物学会第70回大会   (熊本) 

    発表年月: 2006年09月

  • Dynamics of the thylakoid membrane in green leaves of Arabidopsis thaliana

    Nozue, H, Oono, K, Nakanishi, H, Suzuki, K, Kaneko, Y, Sonoike, K, Hayashida, N

    Plant Biology 2006   (Boston, Massachusetts, USA) 

    発表年月: 2006年08月

  • シアノバクテリアの遺伝子機能とクロロフィル蛍光挙動

    園池公毅

    ラン藻ゲノム研究交流会   (東京大学駒場キャンパス) 

    発表年月: 2006年07月

  • パルス変調クロロフィル蛍光測定の原理

    園池公毅

    第14回光合成の反応中心と色素系に関するセミナー   (京都大学) 

    発表年月: 2006年06月

  • Photosynthesis and Chlorophyll Fluorescence

    Sonoike, K

    13th COE seminar of Ryukyu University   (Okinawa,Japan) 

    発表年月: 2006年06月

  • 葉緑体タンパク質の起源となったシアノバクテリア遺伝子の機能解析

    佐藤直樹, 石川正行, 藤原誠, 園池公毅

    日本植物生理学会2006年度年会   (つくば) 

    発表年月: 2006年03月

  • 太平洋型と日本海型ブナにおける光合成電子伝達能の違い

    山崎淳也, 高橋彩子, 園池公毅, 丸田恵美子

    日本植物生理学会2006年度年会   (つくば) 

    発表年月: 2006年03月

  • 遺伝子の注釈の有無とシアノバクテリア遺伝子破壊株のクロロフィル蛍光の関係

    尾崎洋史, 園池公毅

    日本植物生理学会2006年度年会   (つくば) 

    発表年月: 2006年03月

  • ジビニルクロロフィルを蓄積するpcb2変異が光合成とグラナ形成に与える影響

    中西弘充, 野末はつみ, 金子康子, 園池公毅, 橋本昌征, 田口悟朗, 林田信明

    日本植物生理学会2006年度年会   (つくば) 

    発表年月: 2006年03月

  • シアノバクテリアSynechocystis sp. PCC 6803における強光下での光化学系?複合体量はクロロフィルa合成活性依存的に抑制されている

    村松昌幸, 園池公毅, 日原由香子

    日本植物生理学会2006年度年会   (つくば) 

    発表年月: 2006年03月

  • シアノバクテリアの強光順化におけるNblAの役割

    佐藤華代, 園池公毅

    日本植物学会第69回大会   (富山) 

    発表年月: 2005年09月

  • クロロフィル蛍光挙動による藍藻機能未知遺伝子へのアプローチ

    尾崎洋史, 園池公毅

    日本植物学会第69回大会   (富山) 

    発表年月: 2005年09月

  • 重金属輸送に関与するHMAファミリーのシロイヌナズナ新規変異体hma1は過剰な亜鉛に感受性を示す

    樋口美栄子, 園池公毅

    日本植物生理学会2005年度年会   (新潟) 

    発表年月: 2005年03月

  • 系統プロファイリングによる光合成関連遺伝子の推定

    佐藤直樹, 園池公毅, 石川正行, 藤原誠

    日本植物生理学会2005年度年会   (新潟) 

    発表年月: 2005年03月

  • 環境変動下の植物における光合成系の重要性ーイントロダクションー

    園池公毅

    日本植物生理学会2005年度年会   (新潟) 

    発表年月: 2005年03月

  • シアノバクテリアの強光順化のメカニズム

    園池公毅

    大阪大学蛋白質研究所セミナー 葉緑体:構築と分解のメカニズム   (大阪大学吹田キャンパス) 

    発表年月: 2004年11月

  • Regulation of Photosystem I under high-light condition

    Sonoike, K  [招待有り]

    Japanese-Finnish seminar on Molecular Mechanisms of Regulation of Photosynthesis by Environments   (Turku,Finland) 

    発表年月: 2004年11月

  • 光化学系?におけるSQDGの役割

    蓑田歩, 園池公毅, 岡田克彦, 佐藤典裕, 都筑幹夫

    日本植物生理学会2001年度年会   (福岡) 

    発表年月: 2004年09月

  • シアノバクテリアにおける強光応答に欠損のある変異体の解析

    藤森玉輝, 園池公毅

    日本植物学会第68回大会   (藤沢) 

    発表年月: 2004年09月

  • シアノバクテリアにおけるクロロフィル蛍光挙動を用いた遺伝子機能解析

    尾崎洋史, 池内昌彦, 小川晃男, 福澤秀哉, 園池公毅

    日本植物学会第68回大会   (藤沢) 

    発表年月: 2004年09月

  • Non-photochemical quenchingの初期誘導に欠損を示すシロイヌナズナ変異体の解析

    樋口美栄子, 園池公毅

    日本植物学会第68回大会   (藤沢) 

    発表年月: 2004年09月

  • Promoter analysis of genes encoding subunits of Photosystem I in Synechocystis sp. PCC 6803

    Muramatsu, M. Sonoike, K, Hihara, Y

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • Monitoring of fluorescence kinetics in cyanobacterium Synechocystis PCC 6803 for the genome-wide analysis of gene function

    Ozaki, H, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • Characterization of the cyanobacterial mutants that cannot grow under high-light condition

    Fujimori, T, Sato, H, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • Characterization of factors related to photosystem stoichiometry in response to environmental changes in Synechocystis sp. PCC 6803

    Sato, H, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • Characterization of Arabidopsis thaliana mutants with altered nonphotochemical quenching of chlorophyll fluorescence

    Higuchi, M, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • cfa1 is involved in the initial activation of photosynthetic electron transport under high light condition

    Kurosawa, M. Higuchi, M, Kudoh, H, Ozaki, H, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • A PSI subunit, PsaK2, is involved in the regulation of state transition in Synechocystis PCC 6803

    Fujimori, T. Hihara, Y, Sonoike, K

    13th International Congress of Photosynthesis   (Montreal,Canada) 

    発表年月: 2004年08月

  • The factor that regulates photosystem stoichiometry

    Fujimori, T, Higuchi, M, Sato, H. Aiba, H. Muramatsu, M. Hihara, Y, Sonoike, K

    14th International Congress on Photobiology   (Jeju,Korea) 

    発表年月: 2004年06月

  • The Arabidopsis chlorophyll fluorescence alteration (CFA1) mutant has defect in the response of photosynthetic electron transport to high light

    Kurosawa, M, Higuchi, M, Kudoh, H, Sonoike, K

    14th International Congress on Photobiology   (Jeju,Korea) 

    発表年月: 2004年06月

  • Analysis of factors related to photosystem stoichiometry

    Sato, H, Fujimori, T, Sonoike, K

    14th International Congress on Photobiology   (Jeju,Korea) 

    発表年月: 2004年06月

  • 光化学系量比の調節に関わる因子の解析

    佐藤華代, 尾崎洋史, 藤森玉輝, 園池公毅

    日本植物生理学会2004年度年会   (東京) 

    発表年月: 2004年03月

  • シアノバクテリアのゲノムワイドな遺伝子破壊株のクロロフィル蛍光挙動測定

    尾崎洋史, 池内昌彦, 小川晃男, 福澤秀哉, 園池公毅

    日本植物生理学会2004年度年会   (東京) 

    発表年月: 2004年03月

  • シアノバクテリアSynechocystis sp. PCC 6803の新規ストレス応答遺伝子pirABの発現調節機構

    日原由香子, 村松昌幸, 中村絹, 園池公毅

    日本植物生理学会2004年度年会   (東京) 

    発表年月: 2004年03月

  • Mechanisms of acclimation to high-light condition in Synechocystis sp. PCC 6803

    Sonoike, K

    Joint Japanese-Swiss Scientific Seminar: Biogenesis, Function and Acclimation of the Photosynthetic Apparatus   (Okayama,Japan) 

    発表年月: 2003年09月

  • 強光応答に欠損を持つシロイヌナズナ変異体のクロロフィル蛍光挙動による単離

    樋口美栄子, 園池公毅

    日本植物生理学会2003年度年会   (奈良) 

    発表年月: 2003年03月

  • シアノバクテリアにおける強光下で光化学量比の調節ができない変異株の解析

    藤森玉輝, 樋口美栄子, 相場洋志, 園池公毅, 村松昌幸, 日原由香子

    日本植物生理学会2003年度年会   (奈良) 

    発表年月: 2003年03月

  • Genome-wide categorization of photosynthetic and non-photosynthetic genes in Synechocystis PCC 6803 using chlorophyll fluorescence imaging system

    Sonoike, K, Aiba, H, Fujimori, T, Higuchi, M, Ikeuchi, M, Ogawa, A, Fukuzawa, H, Hihara, Y

    Japan-U.S. Cooperative Research Program: Microbial and Plant Metabolism - Function through Genomics   (Maui,Hawaii,USA) 

    発表年月: 2002年11月

  • 二次元蛍光画像解析によるシアノバクテリアのスクリーニング

    相場洋志, 藤森玉輝, 池内昌彦, 小川晃男, 福澤秀哉, 園池公毅

    日本植物学会第66回大会   (京都) 

    発表年月: 2002年09月

  • シロイヌナズナT-DNAタグラインを用いた二次元クロロフィル蛍光画像解析によるスクリーニング

    黒澤真理, 樋口美栄子, 工藤英樹, 風間晴子, 園池公毅

    日本植物学会第66回大会   (京都) 

    発表年月: 2002年09月

  • Photoinhibition and photoprotection of photosystem I

    Sonoike, K

    1st Asian Conference on Photobiology   (Yumebutai,Awaji,Japan) 

    発表年月: 2002年06月

  • Response of Photosystem I to Light Stress

    Sonoike, K

    Symposium on Response of Photosystems of Photosynthesis to Light Stress   (Okayama,Japan) 

    発表年月: 2002年03月

  • 低温・暗処理による酸素発生系の構築過程の解析

    樋口美栄子, 野口巧, 園池公毅

    日本植物生理学会2002年度年会   (岡山) 

    発表年月: 2002年03月

  • 出芽酵母を利用したカロース合成酵素制御因子の探索

    渡辺大輔, 阿部充宏, 関谷(川崎, 真理子, 園池公毅, 大矢禎一

    日本植物生理学会2002年度年会   (岡山) 

    発表年月: 2002年03月

  • Synechocystis sp. PCC 6803のPsaK2サブユニットは強光下でフィコビリゾームから光化学系Iへのエネルギー伝達に関与している

    藤森玉輝, 園池公毅, 日原由香子

    日本植物生理学会2002年度年会   (岡山) 

    発表年月: 2002年03月

  • DNAマイクロアレイによるSynechocystis sp. PCC 6803のレドックス制御遺伝子の探索

    日原由香子, 園池公毅, 金久實, 池内昌彦

    日本植物生理学会2002年度年会   (岡山) 

    発表年月: 2002年03月

  • 低温・光ストレス後にキュウリ葉はなぜ退色するのか?

    工藤英樹, 園池公毅

    日本植物学会第65回大会   (東京) 

    発表年月: 2001年09月

  • シアノバクテリア遺伝子破壊株の網羅的解析−トランスポゾン挿入破壊による全遺伝子産物の機能解析に向けて−

    相場洋志, 池内昌彦, 小川晃男, 福澤秀哉, 園池公毅

    日本植物学会第65回大会   (東京) 

    発表年月: 2001年09月

  • キュウリ生葉内で見られる酸素発生系の不活性化と光による回復過程の解析

    樋口美栄子, 野口巧, 園池公毅

    日本植物学会第65回大会   (東京) 

    発表年月: 2001年09月

  • Photoinhibition of Photosystem I

    Sonoike, K  [招待有り]

    12th international congress on photosynthesis   (Brisbane,Australia) 

    発表年月: 2001年08月

  • Recovery process of photosystem I from chilling-iduced photoinhibition

    Sonoike K, Kudoh H

    Light Stress and Photosynthesis: UV and visible light effects   (Heron Island,Australia) 

    発表年月: 2001年08月

  • DNA microarray analysis of Synechocystis sp. PCC 6803 under conditions of PSII and PSI light

    Hihara Y, Sonoike K, Hiyama T, Ikeuchi M

    Light Stress and Photosynthesis: UV and visible light effects   (Heron Island,Australia) 

    発表年月: 2001年08月

  • 低温・光ストレスによる光化学系Iの不可逆的な阻害

    工藤英樹, 園池公毅

    日本植物生理学会2001年度年会   (福岡) 

    発表年月: 2001年03月

  • シアノバクテリアSynechocystis PCC 6803遺伝子破壊株の蛍光挙動モニタリングによる表現型解析

    相場洋志, 池内昌彦, 小川晃男, 園池公毅

    日本植物生理学会2001年度年会   (福岡) 

    発表年月: 2001年03月

  • キュウリの低温・暗処理による酸素発生系の阻害−熱発光によるマンガンクラスターの解析−

    樋口美栄子, 野口巧, 園池公毅

    日本植物生理学会2001年度年会   (福岡) 

    発表年月: 2001年03月

  • 低温・光ストレスによって阻害された光化学系I反応中心複合体の修復と分解

    工藤英樹, 園池公毅

    日本植物生理学会2000年度年会   (名古屋) 

    発表年月: 2000年03月

  • Physiological significance of the regulation of photosystem stoichiometry upon high-light acclimation of Synechocystis

    Sonoike, K, Hihara, Y, Ikeuchi, M

    Information Exchange Seminar on Photoconversion and Photosynthesis: Past, Present and Future Prospects   (Okazaki,Japan) 

    発表年月: 1999年11月

  • 光化学系Iの光阻害とサイクリック電子伝達の関係

    園池公毅

    日本植物学会第63回大会   (秋田) 

    発表年月: 1999年10月

  • (A) Degradation of photoinhibited reaction center complex after light-chilling stress and (B) Physiological significance of the regulation of photosystem stoichiometry upon high light acclimation of Synechocystis

    Sonoike, K

    Gordon Research Conference: Biochemical Aspects of Photosynthesis   (Henniker,NH,USA) 

    発表年月: 1999年06月

  • シアノバクテリアの強光順化における光化学系量比調節の生理的意義

    園池公毅, 日原由香子, 池内昌彦

    日本植物生理学会1999年度年会   (仙台) 

    発表年月: 1999年03月

  • Cyclic electron transfer is enhanced after light-chilling treatment of cucumber leaves

    Sonoike, K

    Gordon Research Conference: Temperature Stress in Plants   (Harbortown,Ventura,CA,USA) 

    発表年月: 1999年01月

  • Degradation of photosystem I reaction center under light stress

    Sonoike, K

    French-Japan Seminar: Photoscience   (Kanagawa,Japan) 

    発表年月: 1998年11月

  • Photoinhibition of photosynthesis under various stress conditions

    Sonoike, K  [招待有り]

    U.S.-Japan Binational Seminar: The Effects of Environmental Conditions on CO2 Fixation and the Photosynthetic Apparatus   (Asilomer,CA,USA) 

    発表年月: 1998年11月

  • 光・低温ストレス下における光化学系I反応中心サブユニットの分解

    園池公毅

    ユーグレナ研究会シンポジウム「葉緑体研究の新しい流れ」   (堺) 

    発表年月: 1998年11月

  • 光化学系1の光調節

    園池公毅

    日本植物学会第68回大会   (藤沢) 

    発表年月: 1998年09月

  • Photoinhibition of Photosystem I in chilling sensitive plants determined in vivo and in vitro

    Sonoike, K

    XIth International Congress on Photosynthesis   (Budapest,Hungary) 

    発表年月: 1998年08月

  • Effects of virus infection on photosynthesis of Eupatorium makinoi

    Funayama-Noguchi, S, Terashima, I, Sonoike, K

    XIth International Congress on Photosynthesis   (Budapest,Hungary) 

    発表年月: 1998年08月

  • A novel gene, pmgA, specifically regulates photosystem stoichiometry in a cyanobacterium Synechocystis sp. PCC 6803 in response to high light

    Hihara, Y, Sonoike, K, Ikeuchi, M

    XIth International Congress on Photosynthesis   (Budapest,Hungary) 

    発表年月: 1998年08月

  • Different roles of chilling tempratures in the photoinhibition of Photosystem I and Photosystem II

    Sonoike, K

    ESF Workshop: Adverse Effects of Visible and UV Light in Plants and Algae   (Szeged,Hungary) 

    発表年月: 1998年08月

  • 植物の低温光傷害時の系Iおよび系II光阻害において低温の果たす異なった役割

    園池公毅

    日本植物生理学会1998年度年会   (札幌) 

    発表年月: 1998年05月

  • シアノバクテリアSynechocystis sp. PCC6803の強光順化に関わる新規遺伝子pmgAの生理的役割とその遺伝子発現

    日原由香子, 園池公毅, 池内昌彦

    日本植物生理学会1998年度年会   (札幌) 

    発表年月: 1998年05月

  • Studies on the mechanism of cold tolerance in wheat by chlorophyll fluorescence analysis

    Chen, L, Sonoike, K, Ishii, R

    The 3rd Asian crop science conference   (Taichung,Taiwan) 

    発表年月: 1998年04月

  • Photoinhibition of Photosystem I and its relation to chilling sensitivity in plants

    Sonoike, K  [招待有り]

    NIBB Seminar: Regulatory Mechanism of Photosynthesis   (Okazaki,Japan) 

    発表年月: 1998年04月

  • Response pattern of photosynthetic chlorophyll fluorescence to low temperature in Wheat and Maize

    Chen, L, Sonoike, K, Ishii, R

    The 3rd Asia-pacific conference on plant physiology   (Shanghai, China) 

    発表年月: 1997年11月

  • 葉緑素蛍光測定によるコムギとトウモロコシ光合成の低温反応機構の解析

    陳リィ,園池公毅, 石井龍一

    1997年度日本作物学会   (高知) 

    発表年月: 1997年10月

  • クラミドモナスの葉緑体脂質変異株の光合成特性

    佐藤典裕, 蓑田歩, 園池公毅, 川口昭彦, 都築幹夫

    日本植物学会第61回大会   (習志野) 

    発表年月: 1997年09月

  • The mechanism of degradation of PsaB protein, a reaction center subunit of photosystem I, upon photoinhibition

    Sonoike, K, Kamo, M, Hihara, Y, Hiyama, T, Enami, I

    Plant Biology '97   (Vancouver,Canada) 

    発表年月: 1997年08月

  • Different role of chilling temperatures in photoinhibition of PSI and PSII

    Sonoike, K

    Photosynthetic Membranes: Biogenesis and Adaptation   (Vancouver,Canada) 

    発表年月: 1997年07月

  • The Site of chilling-induced inactivation of photosynthesis in chilling-sensitive plants

    Sonoike, K

    International Conference: Molecular Biology and Genetics of Photosynthesis   (Moscow,Russia) 

    発表年月: 1997年06月

  • Photoinhibition of photosystem I

    Sonoike, K

    Photooxidative Damage and Its Relaxation   (Kyoto,Japan) 

    発表年月: 1997年03月

  • Photoinhibition of Photosynthesis at Chilling Temperatures

    Sonoike, K

    The Photosynthesis Seminar of Jerusalem University   (Jerusalem,Israel) 

    発表年月: 1996年10月

  • 光化学系Iとその光阻害のメカニズム

    園池公毅

    日本植物学会第60回大会   (福岡) 

    発表年月: 1996年09月

  • 光化学系I

    園池公毅

    光合成研究者の集いワークショップ「光阻害」   (福岡) 

    発表年月: 1996年09月

  • Irreversible nature of PSI photoinhibition: Photoinhibition of PSII protects PSI from photoinhibition?

    Sonoike, K

    Gordon Research Conference: Photosynthesis -Biochemical Aspects   (New Hampton,NH,USA) 

    発表年月: 1996年08月

  • 低温感受性植物の低温光阻害からの回復

    園池公毅, 石橋百枝, 渡辺昭

    日本植物生理学会1996年度年会   (鹿児島) 

    発表年月: 1996年03月

  • 光合成における不飽和脂肪酸の役割

    佐藤典裕, 園池公毅, 江原友子, 長船哲斎, 都築幹夫, 川口昭彦

    日本植物学会第59回大会   (金沢) 

    発表年月: 1995年09月

  • 光化学系Iの光阻害における活性酸素の関与

    園池公毅

    日本植物学会第59回大会   (金沢) 

    発表年月: 1995年09月

  • The loss of RuBPCase by 24-hour treatment of rain in light

    Ishibashi, M, Terashima, I, Usuda, H, Sonoike, K, Watanabe, A

    Xth International Congress on Photosynthesis   (Montpellier,France) 

    発表年月: 1995年08月

  • Photoinhibition of PSI at chilling temperatures

    Sonoike, K

    Xth International Congress on Photosynthesis   (Montpellier,France) 

    発表年月: 1995年08月

  • Contribution of lipids to PSII

    Sato, N, Sonoike, K, Tsuzuki, M, Kawaguchi, A

    Xth International Congress on Photosynthesis   (Montpellier,France) 

    発表年月: 1995年08月

  • Chilling sensitive steps in leaves of Phaseolus vulgaris L. Examination of the effects of growth irradiances on PSI photoinhibition

    Sonoike, K, Ishibashi, M, Watanabe, A

    Xth International Congress on Photosynthesis   (Montpellier,France) 

    発表年月: 1995年08月

  • Photosystem I as the site of damage in photoinhibition: Possible role of photosystem II down-regulation

    Sonoike, K  [招待有り]

    European Science Foundation Workshop: Visible and UV Light Stress   (Paris,France) 

    発表年月: 1995年08月

  • 被陰条件下に対する陽性植物と陰性植物の葉の呼吸代謝系の比較生理学的研究

    野口航, 園池公毅, 寺島一郎

    日本植物生理学会1995年度年会   (松江) 

    発表年月: 1995年03月

  • 生育光環境による光化学系I光阻害耐性の変化

    園池公毅, 石橋百枝, 渡辺昭

    日本植物生理学会1995年度年会   (松江) 

    発表年月: 1995年03月

  • 光合成におけるスルホキノボシルジアシルグリセロール(SQDG)の役割

    佐藤典裕, 園池公毅, 江原友子, 長船哲斎, 都築幹夫, 川口昭彦

    日本植物生理学会1995年度年会   (松江) 

    発表年月: 1995年03月

  • Mechanism protecting PSI is the chilling sensitive step in chilling sensitive plants

    Sonoike, K

    Gordon Research Conference: Temperature Stress in Plants   (Oxnard,CA,USA) 

    発表年月: 1995年01月

  • Selective photoinhibition of photosystem I in vivo and in vitro

    Sonoike, K  [招待有り]

    The 34th NIBB Conference: Responses of the Photosynthetic Apparatus to Environmental Light Conditions   (Okazaki,Japan) 

    発表年月: 1994年12月

  • Mechanism of photosystem I photoinhibition

    Sonoike, K  [招待有り]

    The Reaction Center of Iron-Sulfur Type in Photosynthesis   (Kanazawa,Japan) 

    発表年月: 1994年12月

  • 光化学系I光阻害の普遍性

    園池公毅

    日本植物学会第58回大会   (札幌) 

    発表年月: 1994年09月

  • キュウリ生葉での光化学系I低温光障害部位

    園池公毅, 寺島一郎, 岩城雅代, 伊藤繁

    日本植物学会第58回大会   (札幌) 

    発表年月: 1994年09月

  • Mechanisms of chilling-induced inactivation of photosynthesis in chilling-sensitive plants

    Sonoike, K, Terashima I  [招待有り]

    U.S.-Japan Binational Seminar: Environmental Stress and Photosynthesis - Physiological and Molecular Approaches   (Kona,Hawaii,USA) 

    発表年月: 1994年03月

  • 低温においてキュウリの葉に光を照射すると光化学系IIではなく光化学系Iが傷み,それが光合成失活の原因となる

    寺島一郎, 舟山幸子, 園池公毅

    日本植物生理学会1994年度年会   (筑波) 

    発表年月: 1994年03月

  • キュウリの葉における光化学系I光阻害のメカニズム

    園池公毅, 寺島一郎

    日本植物生理学会1994年度年会   (筑波) 

    発表年月: 1994年03月

  • 緑藻クラミドモナスの脂質代謝異常変異株の生理生化学的性質

    佐藤典裕, 都築幹夫, 園池公毅, 川口昭彦

    日本生化学会1993年度大会   (東京) 

    発表年月: 1993年10月

  • 液体ヘリウム温度における光合成器官からの熱発光

    野口巧, 井上頼直, 園池公毅

    日本植物生理学会1993年度年会   (金沢) 

    発表年月: 1993年03月

  • Anabaena variabilis ATCC 29413のPsaC欠損変異株からの光化学系I複合体の単離とそのサブユニット組成

    園池公毅, R. Mannar Mannan, 加藤栄, Himadri Pakrasi

    日本植物生理学会1993年度年会   (金沢) 

    発表年月: 1993年03月

  • シアノバクテリアの光化学系I複合体の新しい3.5 kDaタンパク質

    池内昌彦, 園池公毅, 小池裕幸, Himadri Pakrasi, 井上頼直

    日本植物学会第57回大会   (奈良) 

    発表年月: 1992年09月

  • Anabaena 29413の系IサブユニットpsaI遺伝子産物におけるプレ配列の存在

    園池公毅, 池内昌彦, Himadri Pakrasi

    日本植物学会第57回大会   (奈良) 

    発表年月: 1992年09月

  • The function of psaE gene product in reduction of ferredoxin by cyanobacterial PSI reaction center

    Sonoike, K, Hatanaka, H, Katoh, S

    IXth International Congress on Photosynthesis   (Nagoya, Japan) 

    発表年月: 1992年08月

  • Electron transfer from cytochrome c-553 to P-700 in cyanobacterial PS I reaction center complexes with and without the bound psaF gene product

    Hatanaka, H, Sonoike, K, Hirano, M, Katoh, S

    IXth International Congress on Photosynthesis   (Nagoya, Japan) 

    発表年月: 1992年08月

  • Chemical environment around the two chlorophyll a' molecules at the core of Photosystem I

    Maeda, H, Watanabe, T, Sonoike, K

    IXth International Congress on Photosynthesis   (Nagoya, Japan) 

    発表年月: 1992年08月

  • Directed mutagenesis of the cofactor binding proteins of Photosystem I in the cyanobacterium Anabaena variabilis ATCC 29421

    Pakrasi, H.B, Nyhus, K.J, Sonoike, K, Mannan, R.M

    AFRC Robert Hill Symposium on Photosynthesis   (London, UK) 

    発表年月: 1992年02月

  • 光低温処理はキュウリチラコイド膜をuncoupleする

    寺島一郎, 園池公毅, 河津維, 加藤栄

    日本植物生理学会1991年度年会   (岡山) 

    発表年月: 1991年03月

  • 系I反応中心複合体の小型サブユニットの再構成

    園池公毅, 加藤栄

    日本植物生理学会1991年度年会   (岡山) 

    発表年月: 1991年03月

  • シネココッカス系I標品とチトクロ?ムc-553との反応

    畠中秀樹, 園池公毅, 加藤栄

    日本植物学会第55回大会   (静岡) 

    発表年月: 1990年09月

  • 葉緑体熱発光の発光スペクトル

    園池公毅, 小池裕幸, 井上頼直

    日本植物生理学会1990年度年会   (東京) 

    発表年月: 1990年03月

  • ホウレンソウ系I標品のP700差分子吸光係数に対するTriton X-100 の影響

    園池公毅, 加藤栄

    日本植物生理学会1990年度年会   (東京) 

    発表年月: 1990年03月

  • シネココッカス系I標品とフェレドキシンとの反応

    畠中秀樹, 園池公毅, 加藤栄

    日本植物学会第54回大会   (仙台) 

    発表年月: 1989年09月

  • Variations of the differential extinction coefficient of P-700 and re-estimation of stoichiometry of constituents in Photosystem I reaction center complexes from Synechococcus elongatus

    Sonoike, K, Katoh, S

    VIIIth International Congress on Photosynthesis   (Stockholm, Sweden) 

    発表年月: 1989年08月

  • Effects of detergents on the oxidized-minus-reduced differential extinction coefficient of P700

    Sonoike, K, Katoh, S

    International Symposium on Regulation and Efficiency of Photosynthesis   (Shanghai, China) 

    発表年月: 1988年09月

  • P700の差分子吸光係数に対する界面活性剤の影響II

    園池公毅, 加藤栄

    日本植物生理学会1988年度年会   (大阪) 

    発表年月: 1988年04月

  • 好熱性ラン色細菌の系I電子受容体

    園池公毅, 畠中秀樹, 加藤栄

    日本植物学会第53回大会   (岡山) 

    発表年月: 1988年03月

  • 系I反応中心に対する界面活性剤の影響

    園池公毅, 加藤栄

    日本植物生理学会1987年度年会   (浦和) 

    発表年月: 1987年04月

  • P700の差分子吸光係数に対するアクセプターの影響

    園池公毅, 加藤栄

    日本植物学会第51回大会   (鹿児島) 

    発表年月: 1986年10月

  • Effect of detergents on the difference extinction coefficient of P700

    Sonoike, K, Katoh, S

    VIIth International Congress on Photosynthesis   (Rhode Island, USA) 

    発表年月: 1986年08月

  • P700の差分子吸光係数に対する界面活性剤の影響

    園池公毅, 加藤栄

    日本植物生理学会1986年度年会   (仙台) 

    発表年月: 1986年04月

  • シネココッカス系I反応中心標品の構成成分

    園池公毅, 加藤栄

    日本植物学会第50回大会   (新潟) 

    発表年月: 1985年10月

  • 系I反応中心のクロロフィル結合たんぱく

    園池公毅, 加藤栄

    日本植物学会第49回大会   (札幌) 

    発表年月: 1984年08月

  • 系I反応中心複合体の60kDaクロロフィルたんぱく質

    園池公毅, 加藤栄

    日本植物学会第48回大会   (京都) 

    発表年月: 1983年10月

▼全件表示

特定課題研究

  • CAM植物において概日リズムと代謝はどのように相互作用しているのか?

    2021年  

     概要を見る

     CAM植物は、夜と昼と間のエネルギー供給の変化に対応して代謝を切り替えて光合成を行なう。この代謝の切り替えには、当然ながら夜と昼とでの光量の大きな変動による光合成の電子伝達の変化が重要な役割を果たす一方、代謝系の酵素活性の一部は、概日リズムの支配下にあることがわかっている。そこで、実際に概日リズムが、昼夜の光量の変化がない恒暗条件においても、光合成の状態を変化させるのかどうかの検討を行った。光合成の電子伝達の状態を、パルス変調クロロフィル蛍光測定によりモニターしたところ、昼夜の変動により引き起こされる変化の一部は、恒暗条件にしても保たれ、概日リズムが光合成の電子伝達を部分的に制御していることが明らかとなった。

  • クロロフィル蛍光による細胞内代謝推定システムの構築

    2015年  

     概要を見る

    一般に、蛍光測定は光合成の状態をモニターするための非破壊的手段として広く利用されている。しかし、原核生物であるシアノバクテリアは、細胞内のさまざまな代謝が光合成に影響を与える。この代謝系の相互作用を明らかにする途上で、まさにその相互作用が原因となり、細胞内の代謝がクロロフィル蛍光を用いた光合成の測定を妨害する場合があることを発見した。この妨害のメカニズムを解析・検討した結果、青色光の照射による光化学系Ⅰの選択的な励起によってステート遷移の状態を一定に保ち、クロロフィル蛍光の「げた」の部分を補正する計算方法を組み合わせることにより、この妨害を除去して正確な光合成活性を求めることに成功した。

  • 果実の光合成装置の特殊性

    2015年  

     概要を見る

    果実の表面の光合成は葉の光合成とそれほど変わらないが、ソラマメの鞘の中の豆などのように、莢に覆われて直接光があたらない部分の光合成装置はかなり特殊である。今年度の本研究においては、このソラマメの種子の光合成の特殊性が、莢を持つ果実一般に普遍的なものであるのかどうかを検討した。スナップエンドウの莢の透過率は、ソラマメの莢の場合に比べて高く、内部の種子はより明るい環境にあることが推測される。実際にスナップエンドウの種子の光合成を解析した結果、ソラマメでもスナップエンドウでも、種子は葉とは異なる光合成が見られるが、その違いについては種間差があることが明らかとなった。

  • 果実の光合成装置の特殊性

    2014年  

     概要を見る

    It isgenerally assumed that photosynthesis takes place in green leaves. Thephotosynthetic characteristics of the surface of the fruits, however, was notso much different from that of the leaves. On the other hand, photosynthesis ofthe seed coats of the broad been surrounded by pods seems to be ratherpeculiar. In this study, such peculiarity was examined to understand thephysiological significance of photosynthesis in seed coats. The isolation ofthylakoid membranes from the seed coats was difficult, but still possible.Measurements of oxygen evolution and chlorophyll fluorescence emission spectrarevealed that the observed difference of photosynthesis in seed coats was due tothe difference of intrinsic characteristics of photosynthesis in thylakoidmembranes.

  • クロロフィル蛍光による細胞内代謝推定システムの構築

    2012年  

     概要を見る

     光合成は光のエネルギーを化学エネルギーに変換するエネルギー変換系であり、光エネルギーの捕集系としてクロロフィルをはじめとする光合成色素を持っている。光を吸収した色素がそのエネルギーの一部を再び光として放出する過程(蛍光)は、クロロフィルのようにエネルギーが光合成という別の過程によって使われる場合には、それらの過程と競合する。結果として、蛍光と光合成には一般的に負の相関がみられ、蛍光測定は光合成の状態をモニターするための手段として利用可能である。光合成生物の中でも、原核生物であるシアノバクテリアは、細胞内の代謝が複数の細胞小器官に分散していないため、細胞内のさまざまな代謝が原理的には光合成に影響を与えると期待される。このことは、クロロフィル蛍光測定は、光合成以外の代謝についても情報を得る手段としても使える可能性を示唆する。本研究では、クロロフィル蛍光と細胞内の代謝の間の関係を明らかにすることを目的として実験を行なった。 今年度は、光合成以外の代謝系の中で最も解析がしやすいと考えられた呼吸系に焦点を絞り、呼吸の電子伝達に関わるNDH複合体のサブユニットをコードする遺伝子の破壊株の表現型解析を行なった。サブユニットのうちndhF1遺伝子を破壊したシアノバクテリアでは、呼吸速度の低下がみられるとともに、暗順応した細胞に光を照射した際に極めて大きなクロロフィル蛍光強度の変動がみられた。このクロロフィル蛍光挙動の変化の原因を解析したところ、ndhF1遺伝子破壊株においては、暗所でもプラストキノンプールが酸化しており、結果として細胞がステート1に固定されて野生型とは異なる大きなクロロフィル蛍光強度の変化が引き起こされていることが明らかとなった。さらに、このステート状態の変化によって、クロロフィル蛍光から推定される光合成速度が、酸素発生速度によって見積もられる光合成速度と大きく異なってしまうことも明らかとなった。

  • クロロフィル蛍光を用いた新規薬剤スクリーニング法の開発

    2010年  

     概要を見る

    本年度は、シアノバクテリアの特定の蛍光挙動が細胞内のどのような代謝活動に由来しているのかについて研究を行なった。これまでに構築したクロロフィル蛍光挙動のデータベースであるFluoromeを検索することにより、野生型よりも極めて大きなクロロフィル蛍光の変動を示す変異株を見出した。この変異株の原因遺伝子は、呼吸系のNDH複合体のサブユニットの一つであるndhF1であった。シアノバクテリアは呼吸電子伝達鎖と光合成電子伝達鎖がプラストキノンプールを共有しており、光化学系ⅡとNADH脱水素酵素複合体が共にプラストキノンを還元する。呼吸電子伝達鎖が光合成電子伝達に与える影響を明らかにするために、Synechocystis sp. PCC 6803のNADH脱水素酵素複合体の第5サブユニットをコードする遺伝子であるndhF1 (slr0844)を破壊した株を解析した。PAMクロロフィル蛍光測定により求めた生育光強度(20 μmol/m2/s)での光合成電子伝達の実効量子収率ΦⅡはndhF1破壊株の方が野生株よりも高くなっていた。しかしながら、酸素電極を用いて測定した飽和光照射下での光合成電子伝達活性は、野生型とndhF1破壊株で大きな違いは見られなかった。低温クロロフィル蛍光スペクトルの測定によって、野生型は15分間の暗順応によりステート2になるが、ndhF1破壊株では暗順応をしてもステート2になりにくいことが明らかになった。呼吸の末端酸化酵素の阻害剤であるKCNを添加するどちらの株も暗順応でステート2になる。以上の結果は、ndhF1破壊株においては、NADH脱水素酵素複合体の活性低下によりプラストキノンプールがより酸化的になり、ステート2になりにくくなった結果、高い光合成の実効収率を示すと結論できる。

  • 光合成の短期的な光応答メカニズムとその適応的意義

    2010年  

     概要を見る

    本年度は、特定課題の研究期間の短さを考慮し、植物の光環境応答メカニズムのうち、植物がおかれた光環境と光合成の関係に集中して研究を進めた。具体的には、果実のように、内部の光環境が外部とはことなる植物の部位における光合成を中心に研究を行なった。本実験ではクロロフィル蛍光を測定する事によって、果実の部位ごとの光合成活性の差を調べた。ソラマメの豆とさやをパルス変調測定でクロロフィル蛍光測定を行なうと、豆は光化学系Ⅱの最大量子収率の指標であるFv/Fmの値が低くなっていた。この原因を探るために3つの実験を行った。まず1つめは、QAからQBの電子伝達が阻害で、測定パルスによるFOの上昇が起こり、見せかけのFv/Fmが小さくなると考え、LIGHT INTENSITYを変化させて豆とさやそれぞれを測定した。しかし、QAからQBの電子伝達に異常はみられず、豆のFv/Fmが小さい原因ではなかった。次に、豆とさやの電荷分離の反応速度の違いを観察するためにSTflashを用いて測定を行なった。しかし、豆とさやにQAからQBへの電子伝達速度の差異が見られず、これもFv/Fmが小さい原因ではなかった。しかし、系Ⅱのアンテナサイズが同じ事、STflash照射後のQAはさやの方が還元されたままである事、QB以降の電子の流れには違いがある事がわかった。3つめに、系Ⅰが何かしらの事情で増加し、Foが上昇してFv/Fmが小さくなったと考えられる。系Ⅰと系Ⅱの蛍光の違いを調べるために、液体窒素を使用して77Kでの蛍光を測定した。豆とさやで系Ⅰの蛍光の大きさに差は見られなかった。しかし、系Ⅰの波長のピークが豆は短波長側にずれており、豆は系Ⅰのアンテナに異常がある事が推測される。これら3つの実験からQAの還元状態に因るものと判明した。

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    東京大学  

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    名古屋大学  

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    北海道大学  

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    東北大学  

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    東京大学  

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    国際基督教大学  

    2002年
     
     
     

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    東邦大学  

    2000年
     
     
     

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    国際基督教大学  

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  • 現代自然科学とアウトリーチ

    早稲田大学  教員免許更新講習 

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    -
     

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    早稲田大学  教員免許更新講習 

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  • 現代自然科学の現状

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    2013年08月
    -
     

  • 現代自然科学の現状と新指導要領

    早稲田大学  教員免許更新講習 

    2012年08月
    -
     

▼全件表示

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