1. Sixteen polypeptides associated with histopathological differentiation were detected using two-dimensional polyacrylamide gel electrophoresis of primary lung carcinoma.
2. Based on the expression of the polypeptides associated with either histopathological differentiation or cell proliferation, we classified primar lung adenocarcinoma into typical case and atypical case. 65.4% of the typical cases were diagnosed as well differentiated adenocarcinoma histopathologically. Also, 65.4% of the typical cases showed negative in lymph node metastasis. On the other hand, 20.5% and 34.9% of the atypical cases were judged as well differentiated case and cases without lymph node metastasis, respectively. Furthermore, we recognized the statistically significant difference in three year-disease free survival(62.9% in typical cases, 27.1% in atypical cases ; p=0.0011).
3. We produced monoclonal antibodies against N-terminal of TA02 molecule. The histological distribution of TA02 was clarified immunohistochemically using this antibody and human tissues. Also, we obtained the evidence that TA02 molecule was homologos with NapsinA which was a novel aspartic proteinase published at the end of 1998, because the adenocarcinoma cases with high expression of TA02 showed the expression of napsin A in RNA level. Furthermore, GST fusion protein of Napsin A showed positive reaction with anti-TA02 antibody.
4. TS02 associated with neuroendocrine cells was homologous with protein gene product 9.5(PGP 9.5). In the TS02 positive cases two-years prognosis become worse compared to TS02 negative cases.
5. Compred to the 2DE patterns between CDDP-resistant cell line and the parents cell line, the positions of reticulocarbin and glutathione-S-transferase π were identified on 2DE gel. The expession level of reticulocalbin, which was Ca-binding protein at endoplasmic reticulum, became low in the CDDP-resistant cell line. On the other hand, the expression level of GST-πbecame high, remarkably.
6. The analysis based on lung cancer protein using 2DE is valuable for the evaluation of biological tumor malignancy.

1. Sixteen polypeptides associated with histopathological differentiation were detected using two-dimensional polyacrylamide gel electrophoresis of primary lung carcinoma.
2. Based on the expression of the polypeptides associated with either histopathological differentiation or cell proliferation, we classified primar lung adenocarcinoma into typical case and atypical case. 65.4% of the typical cases were diagnosed as well differentiated adenocarcinoma histopathologically. Also, 65.4% of the typical cases showed negative in lymph node metastasis. On the other hand, 20.5% and 34.9% of the atypical cases were judged as well differentiated case and cases without lymph node metastasis, respectively. Furthermore, we recognized the statistically significant difference in three year-disease free survival(62.9% in typical cases, 27.1% in atypical cases ; p=0.0011).
3. We produced monoclonal antibodies against N-terminal of TA02 molecule. The histological distribution of TA02 was clarified immunohistochemically using this antibody and human tissues. Also, we obtained the evidence that TA02 molecule was homologos with NapsinA which was a novel aspartic proteinase published at the end of 1998, because the adenocarcinoma cases with high expression of TA02 showed the expression of napsin A in RNA level. Furthermore, GST fusion protein of Napsin A showed positive reaction with anti-TA02 antibody.
4. TS02 associated with neuroendocrine cells was homologous with protein gene product 9.5(PGP 9.5). In the TS02 positive cases two-years prognosis become worse compared to TS02 negative cases.
5. Compred to the 2DE patterns between CDDP-resistant cell line and the parents cell line, the positions of reticulocarbin and glutathione-S-transferase π were identified on 2DE gel. The expession level of reticulocalbin, which was Ca-binding protein at endoplasmic reticulum, became low in the CDDP-resistant cell line. On the other hand, the expression level of GST-πbecame high, remarkably.
6. The analysis based on lung cancer protein using 2DE is valuable for the evaluation of biological tumor malignancy.

Photodynamic therapy (PDT) is based on incorporation of a photosensitizer that has high affinity for malignant tissue and stimulation of the photosensitizer with a low power laser beam, with tumorcidal effects. Using the same principle fluorescence can also be elicited from the tumor by a laser beam of a different wavelength in order to detect the lesion. Our team has investigated this therapeutic modality in basic and clinical studies for ten years. Our initial studies employed hematoporphyrin derivative (HpD) as the photosensitizer for PDT with an argon dye laser and we have reported the first cases of five-year survival in a case of cancer treated only by PDT.Mono-L-aspartyl chorin e (NPe ) is a photosensitizer newly developed in 1987. This material shows higher affinity to tumor and better light absorption than HpD. It has a strong absorption band at a wavelength longer than 600nm and consequently is not affected by hemoglobin. Further investigations are necessary for clinical application.An excimer dye laser which more powerful than the argon dye laser has been developed for clinical applications. This laser equipment makes it possible to diagnose and treat in the same procedure by a wavelength-change device. Especially for diagnoses, the fiberscopic image, fluorescent light, and fluorescent wave length are shown in the same image.The basic experiment of photodynamic therapy has been performed. HpD and NPe have been shown to have a high affinity for mitochondria. Damage to the mitochondria by photosensitizer was recognized by decrease in mitochondrial movement. This is in agreement with theories that the first step of photodynamic therapy is degeneration of mitochondria.In 1987 the basic investigation, the laser equipment and the newly developed photosensitizer for photodynamic therapy were established. Our proposed project is a clinical trial and comparison with PDT using HpD and argon dye laser, as well as with other modalities